Pub Date : 2026-01-17DOI: 10.1016/j.livsci.2026.105900
Sophie C. McKee , John J. Mayer , Stephanie A. Shwiff , Michael J. Bodenchuk
Background and objectives Wild pigs (Sus scrofa) are notorious to cattle producers in the United States as causing extensive rooting damage to rangeland, transmitting livestock diseases, and preying on domestic calves. Additionally, while wild pigs are known to consume pasture/rangeland forage, the direct impact of that behavior on commercial cattle productivity remains unquantified. This study aimed to calculate wild pig Animal Unit (AU) equivalents, enabling domestic cattle producers to evaluate competition risk of wild pigs for grazing forage resources on their lands.
Data collection Biomass data (in lbs.) for this calculation was drawn from a dataset of 12,257 wild pigs harvested during control efforts at the Savannah River Site (SRS) in South Carolina.
Research methods After reviewing seven studies on wild pig diets in the U.S., a dietary overlap was calculated to determine the percentage of food wild pigs and cattle both consume. We then calculated AU equivalents for wild pigs using two approaches: population size and density. The projected lost market value for beef steers on various sized properties was also calculated.
Results We estimated the average dietary overlap of forage resources between wild pigs and domestic cattle at 50.3%. We determined that 1,498 lbs. of wild pig biomass equal 1 AU, which amounts to 17.7 wild pigs per one AU.
Contributions Using our estimated wild pig AUs equivalents for specific rangeland, producers can make informed management decisions about controlling these invasive competitors. Such decisions will largely depend on property size and local wild pig population density.
{"title":"Invasive wild pigs represent a competitive threat to domestic cattle forage","authors":"Sophie C. McKee , John J. Mayer , Stephanie A. Shwiff , Michael J. Bodenchuk","doi":"10.1016/j.livsci.2026.105900","DOIUrl":"10.1016/j.livsci.2026.105900","url":null,"abstract":"<div><div><strong>Background and objectives</strong> Wild pigs (<em>Sus scrofa</em>) are notorious to cattle producers in the United States as causing extensive rooting damage to rangeland, transmitting livestock diseases, and preying on domestic calves. Additionally, while wild pigs are known to consume pasture/rangeland forage, the direct impact of that behavior on commercial cattle productivity remains unquantified. This study aimed to calculate wild pig Animal Unit (AU) equivalents, enabling domestic cattle producers to evaluate competition risk of wild pigs for grazing forage resources on their lands.</div><div><strong>Data collection</strong> Biomass data (in lbs.) for this calculation was drawn from a dataset of 12,257 wild pigs harvested during control efforts at the Savannah River Site (SRS) in South Carolina.</div><div><strong>Research methods</strong> After reviewing seven studies on wild pig diets in the U.S., a dietary overlap was calculated to determine the percentage of food wild pigs and cattle both consume. We then calculated AU equivalents for wild pigs using two approaches: population size and density. The projected lost market value for beef steers on various sized properties was also calculated.</div><div><strong>Results</strong> We estimated the average dietary overlap of forage resources between wild pigs and domestic cattle at 50.3%. We determined that 1,498 lbs. of wild pig biomass equal 1 AU, which amounts to 17.7 wild pigs per one AU.</div><div><strong>Contributions</strong> Using our estimated wild pig AUs equivalents for specific rangeland, producers can make informed management decisions about controlling these invasive competitors. Such decisions will largely depend on property size and local wild pig population density.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"305 ","pages":"Article 105900"},"PeriodicalIF":1.9,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-16DOI: 10.1016/j.livsci.2026.105899
Thivhilaheli Richard Netshirovha , Arnold Tapera Kanengoni , Michael Chimonyo
This study investigated selenium (Se) and zinc (Zn) supplementation effects on epididymal spermatozoa quality in indigenous Kolbroek and commercial Large White × Landrace (LW × LR) boars. Forty-eight boars, 8 months old at evaluation, were allocated to a 2 × 2 × 2 factorial design with two Se levels (0.26 vs 0.65 mg/kg), two Zn levels (35 vs 74 mg/kg), and two genotypes over 16 weeks. Epididymal spermatozoa were evaluated for concentration, morphology, motility, mineral composition, and oxidative stress markers. The LW × LR boars achieved greater progressive motility with high Se+Zn supplementation (61.8%) versus low supplementation (39.5%; Cohen's d = 1.39), while Kolbroek boars performed optimally under low Se+Zn conditions (43.9% vs 39.7%; Cohen's d = -0.24). Treatment responsiveness was 5.4-fold greater in LW × LR (22.3% range) than Kolbroek (4.1% range). Significant genotype × treatment interactions occurred for live spermatozoa (P < 0.05) and sperm concentration (Se effect: P < 0.05). Sperm concentration showed the largest effect size (Cohen's d = 1.28) with antagonistic Se × Zn interaction tendencies (P = 0.069). Kolbroek boars demonstrated superior mineral retention (23.9% higher selenium) and genotype-dependent oxidative responses. Selenium reduced malondialdehyde by 7.1% in Kolbroek but minimally affected LW × LR, while zinc increased oxidative stress by 14.7% in LW × LR with negligible Kolbroek impact. These findings challenge universal supplementation protocols, showing that indigenous and commercial breeds require different strategies to optimize reproductive performance. The results support implementing breed-specific precision nutrition in pig breeding programs to enhance reproductive efficiency and lower costs.
本试验研究了饲粮中硒（Se）和锌（Zn）对本地Kolbroek公猪和市售LW × LR公猪附睾精子质量的影响。试验选用48头8月龄公猪，采用2 × 2 × 2因子设计，在16周内采用2种硒水平（0.26 vs 0.65 mg/kg）、2种锌水平（35 vs 74 mg/kg）和2种基因型。对附睾精子的浓度、形态、活力、矿物成分和氧化应激标志物进行评价。LW × LR公猪在高硒锌添加量（61.8%）比低硒锌添加量（39.5%,Cohen’s d = 1.39）下具有更大的进进运动能力，而Kolbroek公猪在低硒锌添加量（43.9%比39.7%,Cohen’s d = -0.24）下表现最佳。LW × LR的治疗反应性（22.3%范围）比Kolbroek（4.1%范围）高5.4倍。活精子（P < 0.05）和精子浓度（Se效应：P <； 0.05）存在显著的基因型与处理交互作用。精子浓度与Se × Zn拮抗相互作用趋势的效应最大（Cohen’s d = 1.28） （P = 0.069）。Kolbroek公猪表现出优异的矿物质保留能力（硒含量高出23.9%）和基因型依赖的氧化反应。硒降低了Kolbroek的丙二醛7.1%，但对LW × LR的影响最小；锌增加了LW × LR的氧化应激14.7%，但对Kolbroek的影响可以忽略不计。这些发现挑战了普遍的补充方案，表明本地和商业品种需要不同的策略来优化繁殖性能。研究结果支持在猪育种计划中实施特定品种的精确营养，以提高繁殖效率和降低成本。
{"title":"Effects of selenium and zinc supplementation on epididymal spermatozoa quality in indigenous Kolbroek and Large White boars","authors":"Thivhilaheli Richard Netshirovha , Arnold Tapera Kanengoni , Michael Chimonyo","doi":"10.1016/j.livsci.2026.105899","DOIUrl":"10.1016/j.livsci.2026.105899","url":null,"abstract":"<div><div>This study investigated selenium (Se) and zinc (Zn) supplementation effects on epididymal spermatozoa quality in indigenous Kolbroek and commercial Large White × Landrace (LW × LR) boars. Forty-eight boars, 8 months old at evaluation, were allocated to a 2 × 2 × 2 factorial design with two Se levels (0.26 vs 0.65 mg/kg), two Zn levels (35 vs 74 mg/kg), and two genotypes over 16 weeks. Epididymal spermatozoa were evaluated for concentration, morphology, motility, mineral composition, and oxidative stress markers. The LW × LR boars achieved greater progressive motility with high Se+Zn supplementation (61.8%) versus low supplementation (39.5%; Cohen's d = 1.39), while Kolbroek boars performed optimally under low Se+Zn conditions (43.9% vs 39.7%; Cohen's d = -0.24). Treatment responsiveness was 5.4-fold greater in LW × LR (22.3% range) than Kolbroek (4.1% range). Significant genotype × treatment interactions occurred for live spermatozoa (P < 0.05) and sperm concentration (Se effect: P < 0.05). Sperm concentration showed the largest effect size (Cohen's d = 1.28) with antagonistic Se × Zn interaction tendencies (P = 0.069). Kolbroek boars demonstrated superior mineral retention (23.9% higher selenium) and genotype-dependent oxidative responses. Selenium reduced malondialdehyde by 7.1% in Kolbroek but minimally affected LW × LR, while zinc increased oxidative stress by 14.7% in LW × LR with negligible Kolbroek impact. These findings challenge universal supplementation protocols, showing that indigenous and commercial breeds require different strategies to optimize reproductive performance. The results support implementing breed-specific precision nutrition in pig breeding programs to enhance reproductive efficiency and lower costs.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"305 ","pages":"Article 105899"},"PeriodicalIF":1.9,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-13DOI: 10.1016/j.livsci.2026.105897
Ioannis Brouklogiannis , Aikaterini Koutsaviti , Vassilios Roussis , George Kefalas , Konstantinos C. Mountzouris
This study evaluated the effects of different dietary inclusion levels of a phytogenic blend (PB) containing carvacrol, thymol and olive polyphenols, on productive performance and inflammatory (Nuclear factor-kappa B; NF-κB) responses in the ovary and liver of laying hens. Depending on the PB inclusion level (0, 250, 750, 1000 and 1500 mg/kg feed), 385 Hy-Line Brown hens (21 weeks old) were assigned to 5 dietary treatments: CON, PB250, PB750, PB1000 and PB1500, with 7 replicates of 11 hens, for 12 weeks. Performance responses were recorded weekly, while ovarian and liver tissues were sampled at the 6th and 12th experimental weeks and stored properly for subsequent gene expression analysis. Results showed that dietary PB inclusion improved (P ≤ 0.05) laying rate, egg mass, and FCR compared to the CON treatment, with optimal results evidenced at PB750. The nutrigenomic analysis revealed that PB inclusion (P ≤ 0.05) downregulated most of the inflammatory response–related genes in the ovary, affecting 83% and 75% of the 12 critical genes analyzed at the 6th and 12th experimental weeks, respectively. Similarly, in the liver, PB supplementation consistently reduced (P ≤ 0.05) the expression of about 58% of the NF-κB pathway genes across both time points. In conclusion, PB supplementation beneficially modulated the ovarian and hepatic inflammatory response, providing mechanistic evidence explaining further the improvements observed in laying performance, with the 750 mg/kg inclusion level showing the most consistent benefits.
{"title":"Dietary phytogenic modulation of inflammatory biomarkers in the ovary and liver underlies improved productivity in laying hens","authors":"Ioannis Brouklogiannis , Aikaterini Koutsaviti , Vassilios Roussis , George Kefalas , Konstantinos C. Mountzouris","doi":"10.1016/j.livsci.2026.105897","DOIUrl":"10.1016/j.livsci.2026.105897","url":null,"abstract":"<div><div>This study evaluated the effects of different dietary inclusion levels of a phytogenic blend (PB) containing carvacrol, thymol and olive polyphenols, on productive performance and inflammatory (Nuclear factor-kappa B; NF-κB) responses in the ovary and liver of laying hens. Depending on the PB inclusion level (0, 250, 750, 1000 and 1500 mg/kg feed), 385 Hy-Line Brown hens (21 weeks old) were assigned to 5 dietary treatments: CON, PB250, PB750, PB1000 and PB1500, with 7 replicates of 11 hens, for 12 weeks. Performance responses were recorded weekly, while ovarian and liver tissues were sampled at the 6th and 12th experimental weeks and stored properly for subsequent gene expression analysis. Results showed that dietary PB inclusion improved (<em>P</em> ≤ 0.05) laying rate, egg mass, and FCR compared to the CON treatment, with optimal results evidenced at PB750. The nutrigenomic analysis revealed that PB inclusion (<em>P</em> ≤ 0.05) downregulated most of the inflammatory response–related genes in the ovary, affecting 83% and 75% of the 12 critical genes analyzed at the 6th and 12th experimental weeks, respectively. Similarly, in the liver, PB supplementation consistently reduced (<em>P</em> ≤ 0.05) the expression of about 58% of the NF-κB pathway genes across both time points. In conclusion, PB supplementation beneficially modulated the ovarian and hepatic inflammatory response, providing mechanistic evidence explaining further the improvements observed in laying performance, with the 750 mg/kg inclusion level showing the most consistent benefits.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"304 ","pages":"Article 105897"},"PeriodicalIF":1.9,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taishu horses are an endangered Japanese breed facing challenges due to limited genetic diversity and insufficient profiling. In this study, we aimed to elucidate population genetics for this breed with a particular view to measures of inbreeding and relatedness that will support enhancement of the breed’s genetic diversity. We applied SNP-array techniques to assess inbreeding (runs of homozygosity [ROH], and FIS and FROH indices) and relatedness (kinship, multi-dimensional scaling [MDS], and genetic relationship matrix [GRM]) in nearly all extant Taishu horses as of 2021 (n = 48; age range: 1–27 years). Short ROH accounted for the majority of detected segments (22.75 % of total ROH), while medium to very long ROH were present in all horses, indicating persistent, multi-generational inbreeding. Mean FIS and FROH were -0.053 ± 0.098 and 0.194 ± 0.076, respectively. FIS and FROH were broadly constant, irrespective of year of birth, which indicates a degree of success for the breeding program. Kinship, MDS, and GRM analyses identified close genetic relationships involving one horse of unknown pedigree and confirmed that all 48 individuals formed a single genetic cluster. This study establishes the inbreeding status of Taishu horses and demonstrates the utility of relatedness analyses, which could be used to evaluate suitable candidates for re-introduction into this population, and is of general interest to those involved in conservation efforts for other endangered equine breeds.
{"title":"SNP array-based genomic inbreeding and relatedness analyses in an endangered Japanese equine breed, the Taishu horse","authors":"Tomoko Yoshihara , Teruaki Tozaki , Koki Kawate , Risako Furukawa , Mio Kikuchi , Taichiro Ishige , Hironaga Kakoi , Masaki Takasu , Seiji Hobo","doi":"10.1016/j.livsci.2026.105898","DOIUrl":"10.1016/j.livsci.2026.105898","url":null,"abstract":"<div><div>Taishu horses are an endangered Japanese breed facing challenges due to limited genetic diversity and insufficient profiling. In this study, we aimed to elucidate population genetics for this breed with a particular view to measures of inbreeding and relatedness that will support enhancement of the breed’s genetic diversity. We applied SNP-array techniques to assess inbreeding (runs of homozygosity [ROH], and F<sub>IS</sub> and F<sub>ROH</sub> indices) and relatedness (kinship, multi-dimensional scaling [MDS], and genetic relationship matrix [GRM]) in nearly all extant Taishu horses as of 2021 (<em>n</em> = 48; age range: 1–27 years). Short ROH accounted for the majority of detected segments (22.75 % of total ROH), while medium to very long ROH were present in all horses, indicating persistent, multi-generational inbreeding. Mean F<sub>IS</sub> and F<sub>ROH</sub> were -0.053 ± 0.098 and 0.194 ± 0.076, respectively. F<sub>IS</sub> and F<sub>ROH</sub> were broadly constant, irrespective of year of birth, which indicates a degree of success for the breeding program. Kinship, MDS, and GRM analyses identified close genetic relationships involving one horse of unknown pedigree and confirmed that all 48 individuals formed a single genetic cluster. This study establishes the inbreeding status of Taishu horses and demonstrates the utility of relatedness analyses, which could be used to evaluate suitable candidates for re-introduction into this population, and is of general interest to those involved in conservation efforts for other endangered equine breeds.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"304 ","pages":"Article 105898"},"PeriodicalIF":1.9,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-08DOI: 10.1016/j.livsci.2026.105895
Cathy E. McGettigan , Mark McGee , Edward G. O’Riordan , Alan K. Kelly , Jennifer McMorrow , Bernadette Earley
The objective of this study was to investigate the preference and associated behaviours of finishing beef cattle for different floor types. Two experiments each comprising of forty steers (Experiment 1; 549 kg live weight, SD 10.8; Experiment 2, 564 kg, SD 27.7) were used. For each experiment, animals were weighed on two consecutive days and blocked by breed, age and live weight, and assigned to ten modified experimental pens, with the option to choose between two floor types, of either; i) concrete slatted floor (CS) or ii) concrete slats overlaid with rubber mat (RM) - Experiment 1; and i) RM or ii) CS overlaid with straw (Straw) - Experiment 2; for a duration of 21 days. Animals were offered a total mixed ration based on grass silage plus concentrates ad libitum, along the front of each pen. Animal behaviour in each pen was recorded continuously and the video footage from days 1, 2, 3, 9 and 20 was analysed at 10-minute intervals by a single observer. In Experiment 1, steers consistently preferred the RM floor type (P < 0.001), spending, on average, 77% of their time on RM compared to 23% on CS. When steers selected RM they exhibited a greater percentage of time lying down (P < 0.001), “true” eating, and less time standing idle (P < 0.05), eating drinking, rubbing, auto-grooming (P < 0.001) and allo-grooming (P < 0.01) compared to those on CS. There was no difference in the percentage of time that steers engaged in head-butting on either floor type. In Experiment 2, steers consistently preferred the straw floor type (P < 0.001), spending, on average, 75% of their time on straw compared to 25% on RM. Steers that selected straw spent a greater percentage of time lying down (P < 0.001), and less time standing idle, eating, drinking, rubbing and auto-grooming (P < 0.001) compared to those on RM. There was no difference in the percentage of time that steers engaged in “true” eating, allo-grooming and head-butting on either floor type. In conclusion, finishing beef cattle showed a clear preference for softer flooring surfaces, spending more time lying down.
{"title":"Floor type preference and associated behaviours of finishing beef cattle","authors":"Cathy E. McGettigan , Mark McGee , Edward G. O’Riordan , Alan K. Kelly , Jennifer McMorrow , Bernadette Earley","doi":"10.1016/j.livsci.2026.105895","DOIUrl":"10.1016/j.livsci.2026.105895","url":null,"abstract":"<div><div>The objective of this study was to investigate the preference and associated behaviours of finishing beef cattle for different floor types. Two experiments each comprising of forty steers (Experiment 1; 549 kg live weight, SD 10.8; Experiment 2, 564 kg, SD 27.7) were used. For each experiment, animals were weighed on two consecutive days and blocked by breed, age and live weight, and assigned to ten modified experimental pens, with the option to choose between two floor types, of either; i) concrete slatted floor (CS) or ii) concrete slats overlaid with rubber mat (RM) - Experiment 1; and i) RM or ii) CS overlaid with straw (Straw) - Experiment 2; for a duration of 21 days. Animals were offered a total mixed ration based on grass silage plus concentrates ad libitum<em>,</em> along the front of each pen. Animal behaviour in each pen was recorded continuously and the video footage from days 1, 2, 3, 9 and 20 was analysed at 10-minute intervals by a single observer. In Experiment 1, steers consistently preferred the RM floor type (<em>P</em> < 0.001), spending, on average, 77% of their time on RM compared to 23% on CS. When steers selected RM they exhibited a greater percentage of time lying down (<em>P</em> < 0.001), “true” eating, and less time standing idle (<em>P</em> < 0.05), eating drinking, rubbing, auto-grooming (<em>P</em> < 0.001) and allo-grooming (<em>P</em> < 0.01) compared to those on CS. There was no difference in the percentage of time that steers engaged in head-butting on either floor type. In Experiment 2, steers consistently preferred the straw floor type (<em>P</em> < 0.001), spending, on average, 75% of their time on straw compared to 25% on RM. Steers that selected straw spent a greater percentage of time lying down (<em>P</em> < 0.001), and less time standing idle, eating, drinking, rubbing and auto-grooming (<em>P</em> < 0.001) compared to those on RM. There was no difference in the percentage of time that steers engaged in “true” eating, allo-grooming and head-butting on either floor type. In conclusion, finishing beef cattle showed a clear preference for softer flooring surfaces, spending more time lying down.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"304 ","pages":"Article 105895"},"PeriodicalIF":1.9,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-05DOI: 10.1016/j.livsci.2026.105894
Auvo Sairanen , Elina Juutinen , Torben Larsen , Mogens Vestergaard , Marketta Rinne
Low concentrate input systems can potentially solve some of the environmental and societal challenges of the current high concentrate input dairy production. This study compared a typical high concentrate proportion (47% on dry matter basis) used in intensive Northern European dairy cow feeding with a low concentrate proportion (22%) on a grass silage-based diet. The study lasted over the whole lactation period and used Holstein (n = 32) and Nordic Red (n = 14) dairy cows. A high concentrate proportion significantly increased feed and nutrient intake, while the breed of the cows did not affect nutrient intake and no concentrate proportion × breed interactions were detected. Increased feed intake was reflected in significant increases in daily milk, energy corrected milk, fat, protein, and lactose production. A high concentrate proportion did not alleviate the negative energy balance in the beginning of lactation. No negative effects on cow health were observed related to the low concentrate proportion and both breeds seemed to adapt equally well to the low concentrate proportion. Based on current feed prices in Finland, a high concentrate proportion was economically more viable.
{"title":"Responses of Nordic Red and Holstein dairy cows to reduced concentrate feed input on a grass silage-based diet","authors":"Auvo Sairanen , Elina Juutinen , Torben Larsen , Mogens Vestergaard , Marketta Rinne","doi":"10.1016/j.livsci.2026.105894","DOIUrl":"10.1016/j.livsci.2026.105894","url":null,"abstract":"<div><div>Low concentrate input systems can potentially solve some of the environmental and societal challenges of the current high concentrate input dairy production. This study compared a typical high concentrate proportion (47% on dry matter basis) used in intensive Northern European dairy cow feeding with a low concentrate proportion (22%) on a grass silage-based diet. The study lasted over the whole lactation period and used Holstein (<em>n</em> = 32) and Nordic Red (<em>n</em> = 14) dairy cows. A high concentrate proportion significantly increased feed and nutrient intake, while the breed of the cows did not affect nutrient intake and no concentrate proportion × breed interactions were detected. Increased feed intake was reflected in significant increases in daily milk, energy corrected milk, fat, protein, and lactose production. A high concentrate proportion did not alleviate the negative energy balance in the beginning of lactation. No negative effects on cow health were observed related to the low concentrate proportion and both breeds seemed to adapt equally well to the low concentrate proportion. Based on current feed prices in Finland, a high concentrate proportion was economically more viable.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"304 ","pages":"Article 105894"},"PeriodicalIF":1.9,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-01DOI: 10.1016/j.livsci.2026.105884
Jianfei Zhao , Zhongqian Lu , Shan Du , Yufei Zhu , Shanchuan Cao , Jingbo Liu
We systematically investigated dietary Zanthoxylum bungeanum leaf (ZBL) effects on performance, carcass traits, meat quality, serum and muscle metabolomic profiles, and gut microbiota composition in Daheng broiler chicken. A total of 512 healthy 50-day-old male chickens with similar body weights (1697.35±11.08 g) were randomly assigned to one of four dietary treatments: a control group (0% ZBL) and three ZBL-supplemented groups (2%, 3%, and 4% ZBL). Each group contained eight replicates (16 chickens/replicate) and the trial lasted 37 days. ZBL supplementation (4%) significantly reduced final weight (FW) and average daily gain (ADG) (P< 0.05) while it increased feed-to-gain ratios (F/G) (P< 0.01), compared to the control group. ZBL also significantly reduced drip loss, cooking loss, and malondialdehyde (MDA) content in breast muscle (P< 0.01), while it significantly increased total antioxidant capacity (T-AOC) and catalase (CAT) activity (P < 0.01). Gut microbiota analysis revealed no change in α-diversity at 3% ZBL, but linear discriminant analysis effect size (LEfSe) identified enriched Archaea (P < 0.05). Serum metabolomics analysis detected 38 differentially abundant metabolites (nucleotides, lipids) between control and 3% ZBL groups. Muscle metabolomics indicated elevated amino acid, carbohydrate, and lipid pathway activity in 3% ZBL groups. Correlation analysis further showed that serum and muscle metabolite changes were closely associated with intestinal flora roles, and thus affected phenotypic indicators. In summary, dietary 3% ZBL is optimal for enhancing meat quality via antioxidant enhancement, gut microbiota modulation, and metabolite alterations without compromising growth performance. Although 4% ZBL further improved meat quality, its growth inhibition limits its practical application.
{"title":"Dietary Zanthoxylum bungeanum leaf supplementation improves meat quality of Daheng broiler chicken by altering gut microbiota-serum-muscle metabolome axis","authors":"Jianfei Zhao , Zhongqian Lu , Shan Du , Yufei Zhu , Shanchuan Cao , Jingbo Liu","doi":"10.1016/j.livsci.2026.105884","DOIUrl":"10.1016/j.livsci.2026.105884","url":null,"abstract":"<div><div>We systematically investigated dietary <em>Zanthoxylum bungeanum</em> leaf (ZBL) effects on performance, carcass traits, meat quality, serum and muscle metabolomic profiles, and gut microbiota composition in Daheng broiler chicken. A total of 512 healthy 50-day-old male chickens with similar body weights (1697.35±11.08 g) were randomly assigned to one of four dietary treatments: a control group (0% ZBL) and three ZBL-supplemented groups (2%, 3%, and 4% ZBL). Each group contained eight replicates (16 chickens/replicate) and the trial lasted 37 days. ZBL supplementation (4%) significantly reduced final weight (FW) and average daily gain (ADG) (<em>P</em> <em><</em> 0.05) while it increased feed-to-gain ratios (F/G) (<em>P</em> <em><</em> 0.01), compared to the control group. ZBL also significantly reduced drip loss, cooking loss, and malondialdehyde (MDA) content in breast muscle (<em>P</em> <em><</em> 0.01), while it significantly increased total antioxidant capacity (T-AOC) and catalase (CAT) activity (<em>P</em> < 0.01). Gut microbiota analysis revealed no change in α-diversity at 3% ZBL, but linear discriminant analysis effect size (LEfSe) identified enriched <em>Archaea</em> (<em>P</em> < 0.05). Serum metabolomics analysis detected 38 differentially abundant metabolites (nucleotides, lipids) between control and 3% ZBL groups. Muscle metabolomics indicated elevated amino acid, carbohydrate, and lipid pathway activity in 3% ZBL groups. Correlation analysis further showed that serum and muscle metabolite changes were closely associated with intestinal flora roles, and thus affected phenotypic indicators. In summary, dietary 3% ZBL is optimal for enhancing meat quality via antioxidant enhancement, gut microbiota modulation, and metabolite alterations without compromising growth performance. Although 4% ZBL further improved meat quality, its growth inhibition limits its practical application.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"304 ","pages":"Article 105884"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-01DOI: 10.1016/j.livsci.2025.105873
Breanna M. Roque , Amelia K. Almeida , Marina R.S. Fortes , Chiara Palmieri , Robert D. Kinley
Asparagopsis supplementation induces strong antimethanogenic effects in ruminant animals by consistently reducing enteric methane (CH4) emissions in controlled feeding environments. This study has advanced the utility of feeding dried Asparagopsis products (Asp-Meal) in beef feedlots. The experiment was conducted at an Australian-certified feedlot, using Greenfeed emissions monitoring, and 64 heifers of mixed breeds (aged 12 – 18 months, initial BW 384 ± 27.8 kg) were offered diets of Control (no Asp-Meal) or Asp-Meal [30 mg bromoform/kg DMI] for 74-days. A pilot study was used to determine the inclusion rate of 30 mg bromoform/kg dry matter intake (DMI). Asp-Meal inclusion induced CH4 mitigation of 67.9 % for production (g/day), 67.0 % for yield (g/kg DMI), and 61.7 % for intensity (g/kg average daily weight gain), however, it should be noted that Asp-Meal CH4 measurements were consistently lower than the limit of detection (40 g/d) of the Greenfeed sensors which could have confounded antimethanogenic accounting. Additionally, no negative impacts were induced on animal growth performance, carcass quality, or rumen epithelium confirmed with macroscopic gross and histological examinations. No bromoform was found in meat, kidney, or liver of either Control or Asp-Meal cattle. Iodide and bromide levels for Control and Asp-Meal groups were within acceptable daily intake ranges for human consumption, however, were elevated by Asp-Meal in meat, kidney, and liver. Commercial application of Asparagopsis products for feedlots would benefit from elucidation of breed effect on enteric CH4 reduction and productivity therefore variable breed studies are warranted. This study demonstrates that Asp-Meal is an efficient antimethanogenic feed additive for methane emissions management from feedlot beef production without negative impact on production or food quality.
{"title":"Asparagopsis meal reduces enteric methane emissions of feedlot beef cattle without impact on productivity or meat and carcass quality","authors":"Breanna M. Roque , Amelia K. Almeida , Marina R.S. Fortes , Chiara Palmieri , Robert D. Kinley","doi":"10.1016/j.livsci.2025.105873","DOIUrl":"10.1016/j.livsci.2025.105873","url":null,"abstract":"<div><div><em>Asparagopsis</em> supplementation induces strong antimethanogenic effects in ruminant animals by consistently reducing enteric methane (CH<sub>4</sub>) emissions in controlled feeding environments. This study has advanced the utility of feeding dried <em>Asparagopsis</em> products (Asp-Meal) in beef feedlots. The experiment was conducted at an Australian-certified feedlot, using Greenfeed emissions monitoring, and 64 heifers of mixed breeds (aged 12 – 18 months, initial BW 384 ± 27.8 kg) were offered diets of Control (no Asp-Meal) or Asp-Meal [30 mg bromoform/kg DMI] for 74-days. A pilot study was used to determine the inclusion rate of 30 mg bromoform/kg dry matter intake (DMI). Asp-Meal inclusion induced CH<sub>4</sub> mitigation of 67.9 % for production (g/day), 67.0 % for yield (g/kg DMI), and 61.7 % for intensity (g/kg average daily weight gain), however, it should be noted that Asp-Meal CH<sub>4</sub> measurements were consistently lower than the limit of detection (40 g/d) of the Greenfeed sensors which could have confounded antimethanogenic accounting. Additionally, no negative impacts were induced on animal growth performance, carcass quality, or rumen epithelium confirmed with macroscopic gross and histological examinations. No bromoform was found in meat, kidney, or liver of either Control or Asp-Meal cattle. Iodide and bromide levels for Control and Asp-Meal groups were within acceptable daily intake ranges for human consumption, however, were elevated by Asp-Meal in meat, kidney, and liver. Commercial application of <em>Asparagopsis</em> products for feedlots would benefit from elucidation of breed effect on enteric CH<sub>4</sub> reduction and productivity therefore variable breed studies are warranted. This study demonstrates that Asp-Meal is an efficient antimethanogenic feed additive for methane emissions management from feedlot beef production without negative impact on production or food quality.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"303 ","pages":"Article 105873"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145879951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-30DOI: 10.1016/j.livsci.2025.105883
Kaique Moreira Gomes , Jean Kaique Valentim , Carlos Henrique de Oliveira , Artur Macedo Ribeiro , Kelly Morais Maia Dias , Arele Arlindo Calderano , Jansller Luiz Genova , Tarciso Tizziani , Melissa Izabel Hannas
<div><div>An experiment was conducted to evaluate the effects of phytase (PHY; g/ton) with carbohydrases (CBH; g/ton) and protease (PRO; g/ton) on growth performance, body composition, carcass and cuts traits, jejunal morphology, and economic viability of broiler chickens have fed diets, which had a reduction in the metabolizable energy (ME), available phosphorous (AvP), crude protein (CP), and total calcium (Ca). A total of 1400 male chicks were randomly assigned to seven treatments, and 10 replicates of 20 broiler chickens per pen. Data-variance analysis, and orthogonal comparisons have been conducted. Treatments included the positive control (PC), NC1 (the ME, AvP, and Ca in the NC1 diets respectively were 100 kcal/kg, 0.18 %, and 0.20 % lower than those PC), NC2 (the percentages of CP, AvP, and Ca in the NC2 diets respectively were 1.5 %, 0.18 %, and 0.20 % smaller than those PC), NC3 (the ME, CP, AvP, and Ca in the NC3 diets respectively were 100 kcal/kg, 1.5 %, 0.18 %, and 0.20 % lower than those PC), NC1 + PHY + CBH (CPNC1), NC2 + PHY + PRO (PPNC2), and NC3 + PHY + CBH + PRO (CPPNC3). Feed intake (FI), and body weight gain (BWG) in the starter and entire phases, the soft-lean-tissue (SLT), body-protein (BP), body-water (BW), and body-ash (BA) weights, the preslaughter live-body weight (LBW), the relative weights of carcass, breast, and legs (CBL), the jejunal villi height to crypt depth ratio (VH:CD), and feed cost (FC; USA$/chick) of broiler chickens fed PC were higher than those of the average of three NC diets without enzymes (ANC) (<em>P</em> <em>≤</em> 0.05). However, either feed conversion ratio (FCR) in both phases, CD, and feed cost (FCB; USA$/kg of BWG) stem from feeding PC or FI in both phases, the starter-phase FCR, VH, FC, and FCB stem from feeding PC or the entire-phase FCR, and FCB stem from feeding NC2 respectively were lower than the same items stem from feeding ANC, the average of three NC diets with enzyme (ANCE), and PPNC2 (<em>P</em> <em>≤</em> 0.05). Also, FI, and BWG in both phases, BA, LBW, CBL, and FC stem from feeding NC1, NC2, NC3, and ANC respectively were smaller than those of CPNC1, PPNC2, CPPNC3, and ANCE (<em>P</em> <em>≤</em> 0.05). Similarly, FI, and FCR in both phases stem from feeding CPNC1, and PPNC2 were smaller than those of CPPNC3 (<em>P</em> <em>≤</em> 0.05). However, either FCR in both phases, CD, and FCB stem from feeding ANC or the starter-phase FCR, and CD stem from feeding NC3 or the entire-phase FI stem from feeding CPNC1 respectively were greater than the same items stem from feeding ANCE, CPPNC3, and PPNC2 (<em>P</em> <em>≤</em> 0.05). Also, SLT, BP, and BW stem from feeding NC2, NC3, and ANC respectively were lower than those of PPNC2, CPPNC3, and ANCE (<em>P</em> <em>≤</em> 0.05). Similarly, either VH, VH:CD, and the body-fat weight stem from feeding ANC or VH:CD, and the body-fat weight stem from feeding NC3 or VH, and VH:CD stem from feeding NC1 respectively were smaller than the same ite
{"title":"Supplementation of multi enzymes mitigates nutrient and energy restriction effects in broiler chickens","authors":"Kaique Moreira Gomes , Jean Kaique Valentim , Carlos Henrique de Oliveira , Artur Macedo Ribeiro , Kelly Morais Maia Dias , Arele Arlindo Calderano , Jansller Luiz Genova , Tarciso Tizziani , Melissa Izabel Hannas","doi":"10.1016/j.livsci.2025.105883","DOIUrl":"10.1016/j.livsci.2025.105883","url":null,"abstract":"<div><div>An experiment was conducted to evaluate the effects of phytase (PHY; g/ton) with carbohydrases (CBH; g/ton) and protease (PRO; g/ton) on growth performance, body composition, carcass and cuts traits, jejunal morphology, and economic viability of broiler chickens have fed diets, which had a reduction in the metabolizable energy (ME), available phosphorous (AvP), crude protein (CP), and total calcium (Ca). A total of 1400 male chicks were randomly assigned to seven treatments, and 10 replicates of 20 broiler chickens per pen. Data-variance analysis, and orthogonal comparisons have been conducted. Treatments included the positive control (PC), NC1 (the ME, AvP, and Ca in the NC1 diets respectively were 100 kcal/kg, 0.18 %, and 0.20 % lower than those PC), NC2 (the percentages of CP, AvP, and Ca in the NC2 diets respectively were 1.5 %, 0.18 %, and 0.20 % smaller than those PC), NC3 (the ME, CP, AvP, and Ca in the NC3 diets respectively were 100 kcal/kg, 1.5 %, 0.18 %, and 0.20 % lower than those PC), NC1 + PHY + CBH (CPNC1), NC2 + PHY + PRO (PPNC2), and NC3 + PHY + CBH + PRO (CPPNC3). Feed intake (FI), and body weight gain (BWG) in the starter and entire phases, the soft-lean-tissue (SLT), body-protein (BP), body-water (BW), and body-ash (BA) weights, the preslaughter live-body weight (LBW), the relative weights of carcass, breast, and legs (CBL), the jejunal villi height to crypt depth ratio (VH:CD), and feed cost (FC; USA$/chick) of broiler chickens fed PC were higher than those of the average of three NC diets without enzymes (ANC) (<em>P</em> <em>≤</em> 0.05). However, either feed conversion ratio (FCR) in both phases, CD, and feed cost (FCB; USA$/kg of BWG) stem from feeding PC or FI in both phases, the starter-phase FCR, VH, FC, and FCB stem from feeding PC or the entire-phase FCR, and FCB stem from feeding NC2 respectively were lower than the same items stem from feeding ANC, the average of three NC diets with enzyme (ANCE), and PPNC2 (<em>P</em> <em>≤</em> 0.05). Also, FI, and BWG in both phases, BA, LBW, CBL, and FC stem from feeding NC1, NC2, NC3, and ANC respectively were smaller than those of CPNC1, PPNC2, CPPNC3, and ANCE (<em>P</em> <em>≤</em> 0.05). Similarly, FI, and FCR in both phases stem from feeding CPNC1, and PPNC2 were smaller than those of CPPNC3 (<em>P</em> <em>≤</em> 0.05). However, either FCR in both phases, CD, and FCB stem from feeding ANC or the starter-phase FCR, and CD stem from feeding NC3 or the entire-phase FI stem from feeding CPNC1 respectively were greater than the same items stem from feeding ANCE, CPPNC3, and PPNC2 (<em>P</em> <em>≤</em> 0.05). Also, SLT, BP, and BW stem from feeding NC2, NC3, and ANC respectively were lower than those of PPNC2, CPPNC3, and ANCE (<em>P</em> <em>≤</em> 0.05). Similarly, either VH, VH:CD, and the body-fat weight stem from feeding ANC or VH:CD, and the body-fat weight stem from feeding NC3 or VH, and VH:CD stem from feeding NC1 respectively were smaller than the same ite","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"304 ","pages":"Article 105883"},"PeriodicalIF":1.9,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-22DOI: 10.1016/j.livsci.2025.105882
Elaine Magnani , Laura B. Toseti , Eduardo M. Paula , Marcos I. Marcondes , Pedro D.B. Benedeti , Thiago H. da Silva , Alexandre Berndt , Paulo R. Leme , Renata H. Branco
This study evaluated the effects of forage-derived neutral detergent fiber (NDFf) levels and essential oils as a natural alternative to monensin on feed intake, performance, carcass characteristics, enteric methane (CH4) emissions, and ruminal protozoa in Nellore bulls finished under tropical conditions. Sixty Nellore bulls (409 ± 6.36 kg body weight; 24 months old) were assigned to one of six treatments in a 2 × 3 factorial design. Treatments included two feed additives, monensin (30 mg/kg DM) or a blend of essential oils from castor oil and cashew nutshell liquid (500 mg/kg DM), combined with diets containing 6 %, 9 %, or 15 % NDFf. Monensin supplementation significantly increased final body weight, carcass weight, average daily gain (ADG), dry matter intake (DMI), feed efficiency, metabolic energy requirements, carcass length, renal fat deposition, and empty gastrointestinal tract weight (P < 0.05). In contrast, essential oil supplementation resulted in a larger ribeye area, higher concentrations of n-3 fatty acids in meat, and reduced daily CH4 emissions (P < 0.05), indicating potential benefits for meat quality and environmental sustainability. Although monensin reduced protozoal counts, it was less effective than essential oils in mitigating CH4 emissions. Increasing NDFf levels led to higher DMI, CH4 emissions, gross energy intake, and CH4/DMI ratio (P < 0.05). Bulls fed diets with 15 % NDFf exhibited the longest carcasses and heaviest gastrointestinal tracts (P < 0.01). Overall, these results underscore the trade-offs between maximizing performance, maintaining rumen health, and minimizing environmental impact. While monensin remains a potent tool for improving feedlot efficiency, essential oils offer promising benefits for sustainable beef production, particularly when integrated into well-balanced diets with optimized fiber levels.
{"title":"Influence of monensin, essential oils, and forage-derived neutral detergent fiber levels on performance, carcass traits, methane emissions, and rumen protozoa in Nellore bulls","authors":"Elaine Magnani , Laura B. Toseti , Eduardo M. Paula , Marcos I. Marcondes , Pedro D.B. Benedeti , Thiago H. da Silva , Alexandre Berndt , Paulo R. Leme , Renata H. Branco","doi":"10.1016/j.livsci.2025.105882","DOIUrl":"10.1016/j.livsci.2025.105882","url":null,"abstract":"<div><div>This study evaluated the effects of forage-derived neutral detergent fiber (NDF<sub>f</sub>) levels and essential oils as a natural alternative to monensin on feed intake, performance, carcass characteristics, enteric methane (CH<sub>4</sub>) emissions, and ruminal protozoa in Nellore bulls finished under tropical conditions. Sixty Nellore bulls (409 ± 6.36 kg body weight; 24 months old) were assigned to one of six treatments in a 2 × 3 factorial design. Treatments included two feed additives, monensin (30 mg/kg DM) or a blend of essential oils from castor oil and cashew nutshell liquid (500 mg/kg DM), combined with diets containing 6 %, 9 %, or 15 % NDF<sub>f</sub>. Monensin supplementation significantly increased final body weight, carcass weight, average daily gain (ADG), dry matter intake (DMI), feed efficiency, metabolic energy requirements, carcass length, renal fat deposition, and empty gastrointestinal tract weight (<em>P</em> < 0.05). In contrast, essential oil supplementation resulted in a larger ribeye area, higher concentrations of n-3 fatty acids in meat, and reduced daily CH<sub>4</sub> emissions (<em>P</em> < 0.05), indicating potential benefits for meat quality and environmental sustainability. Although monensin reduced protozoal counts, it was less effective than essential oils in mitigating CH<sub>4</sub> emissions. Increasing NDF<sub>f</sub> levels led to higher DMI, CH<sub>4</sub> emissions, gross energy intake, and CH<sub>4</sub>/DMI ratio (<em>P</em> < 0.05). Bulls fed diets with 15 % NDF<sub>f</sub> exhibited the longest carcasses and heaviest gastrointestinal tracts (<em>P</em> < 0.01). Overall, these results underscore the trade-offs between maximizing performance, maintaining rumen health, and minimizing environmental impact. While monensin remains a potent tool for improving feedlot efficiency, essential oils offer promising benefits for sustainable beef production, particularly when integrated into well-balanced diets with optimized fiber levels.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"304 ","pages":"Article 105882"},"PeriodicalIF":1.9,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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