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Genetic parameters for litter and carcass traits in Landrace and Yorkshire pigs 长白猪和约克郡猪产仔和胴体性状的遗传参数
IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-10-23 DOI: 10.1016/j.livsci.2025.105843
Iasmin Marques Rocha , Giovanna Maria dos Santos Câmara , Maria Victória Henrique Genuíno , Ayrton Fernandes de Oliveira Bessa , Roney Teixeira , Luis Artur Loyola Chardulo , Rogério Abdallah Curi , Welder Angelo Baldassini , Guilherme Luis Pereira , Daniela do Amaral Grossi , Marcos Eli Buzanskas
Swine production has focused on improving litter size and carcass traits to enhance productivity. Genetic progress in traits such as the number of piglets born alive (NBA), birth weight (BW), number of piglets weaned (NPW), total teat number (TN), number of days to reach 100 kg (AGE100), backfat thickness (BF), and loin depth (LD) is of great importance for breeding efficiency. The present study aimed to estimate genetic parameters, including heritability and genetic correlations, for the mentioned traits in Landrace and Yorkshire pigs. Genetic parameters were estimated using the restricted maximum likelihood method, considering an animal model. Heritability estimates ranged from 0.06 (NPW) to 0.41 (BW) in Landrace and 0.04 (NPW) to 0.40 (BW) in Yorkshire. Maternal heritability and permanent environmental effects were low, with values between 0.02 and 0.05. The maternal permanent environmental variance ranged between 0.03 and 0.09. Repeatability estimates for all traits ranged from 0.10 (NPW) to 0.49 (BW). All the traits studied showed sufficient additive genetic variability, enabling effective selection in Landrace and Yorkshire pigs. Greater relative responses are expected for BF, BW, NBA, LD, and TN. Selection for BW may yield favorable correlated responses with NPW, TN, and AGE100, while direct selection for TN may enhance sow reproductive performance. Genetic correlations between BF and LD indicated that both traits can be improved simultaneously without unfavorable effects. Although the correlation between NBA and BW was not high, caution is warranted regarding the increase in litter size due to potential negative consequences, such as reduced birth weight and higher piglet mortality. Despite previous investigations on these traits, this study provides updated and robust estimates from a large, consistent, and environmentally controlled population, representing a valuable case study for commercial swine breeding programs.
养猪生产的重点是提高产仔数和胴体性状,以提高生产力。产活仔数(NBA)、初生重(BW)、断奶仔数(NPW)、总泌乳数(TN)、达到100 kg的天数(AGE100)、背膘厚(BF)和腰深(LD)等性状的遗传进展对育种效率具有重要意义。本研究旨在估计长白猪和约克郡猪上述性状的遗传参数,包括遗传力和遗传相关性。考虑动物模型,采用限制最大似然法估计遗传参数。长白遗传力为0.06 ~ 0.41 (BW),约克郡遗传力为0.04 ~ 0.40 (BW)。母系遗传力和永久环境效应较低,值在0.02 ~ 0.05之间。母系永久环境方差在0.03 ~ 0.09之间。所有性状的重复性估计范围为0.10 (NPW) ~ 0.49 (BW)。所研究的性状均表现出充分的加性遗传变异,使长白猪和约克猪能够进行有效的选择。预期BF、BW、NBA、LD和TN的相对响应更大。选择BW可能与NPW、TN和AGE100产生良好的相关响应,而直接选择TN可能提高母猪的繁殖性能。BF和LD的遗传相关性表明,这两个性状可以同时改良而不会产生不利影响。虽然NBA和体重之间的相关性不高,但由于潜在的负面影响,如出生体重降低和仔猪死亡率升高,因此需要谨慎对待产仔数的增加。尽管之前对这些性状进行了调查,但本研究提供了来自大型、一致和环境控制的种群的最新和可靠的估计,代表了对商业猪养殖计划的有价值的案例研究。
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引用次数: 0
A rapid laboratory method for estimating the standardised precaecal digestible amino acids in pig feeds 猪饲料中标准化粪前可消化氨基酸的实验室快速测定方法
IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-10-16 DOI: 10.1016/j.livsci.2025.105841
Valérie Schumacher , Markus Rodehutscord , Karl-Heinz Südekum , Saskia Kehraus
Amino acids (AA) are essential nutrients for diverse processes in the pig’s body. The utilisation of AA depends on their digestibility and absorption. Therefore, methods to determine reliably the AA supply to pigs to sustain performance and animal health are critical for precise feed evaluation. The evaluation of AA supply has so far been based on in vivo determination of standardised precaecal digestible (pcd) AA (pcdAA) and in vitro estimates of pcdAA applying time-consuming and complex laboratory methods. The objective of this study was to develop and establish a rapid laboratory method for estimating pcdAA based on the determination of AA insoluble in neutral-detergent (ND) or acid-detergent (AD) (NDIAA, ADIAA). The laboratory method used the same procedure which was previously applied to estimate standardised pcd crude protein (pcdCP). The hypothesis was that the method was similarly suitable to estimate pcdAA. A sample pool of 74 feed ingredients (cereal grains, differently heat-treated legume grains) was available on which in vivo pcdAA were determined in cannulated pigs. Amino acids in feed ingredients and in ND or AD residues of feed ingredients were determined by an HPLC method. The concentrations (g/kg dry matter) of ND- and AD-soluble AA (NDSAA, ADSAA) were calculated by difference to total AA in feed. For the estimation of the concentrations of in vivo pcdAA for total AA and the entire dataset (n = 74), a linear relationship was established between the concentrations of NDSAA or ADSAA and the in vivo pcdAA: y = 0.823 (standard error [SE] 0.018) x + 10.52 (SE 4.420), where y represents the in vivo pcdAA (g/kg dry matter) and x represents the NDSAA (cereal grains) or ADSAA (protein feeds) value (g/kg dry matter). The coefficient of determination (R²) of this equation was 0.968 and ranged from 0.895 to 0.984 for the 17 individual AA. This study shows that in vivo pcdAA values can be estimated following the same standardised and rapid laboratory procedure previously established for pcdCP, based on chemical analyses, namely determination of NDIAA and ADIAA, from which NDSAA and ADSAA values are calculated.
氨基酸(AA)是猪体内各种过程的必需营养素。AA的利用取决于它们的消化率和吸收率。因此,确定可靠的AA供应以维持猪的生产性能和动物健康的方法对于精确的饲料评估至关重要。到目前为止,对AA供应的评估是基于标准化的粪前可消化(pcd) AA (pcdAA)的体内测定,以及采用耗时且复杂的实验室方法对pcdAA的体外估计。本研究的目的是在测定不溶于中性洗涤剂(ND)或酸性洗涤剂(AD)的AA (NDIAA, ADIAA)的基础上,建立一种快速测定pcdAA的实验室方法。实验室方法使用了先前用于估计标准化pcd粗蛋白(pcdCP)的相同程序。假设该方法同样适用于估计pcdAA。采用74种饲料原料(谷物、不同热处理的豆科谷物)作为样品池,对空心猪体内pcdAA进行了测定。采用高效液相色谱法测定饲料原料中的氨基酸和饲料原料中ND或AD残留量。通过与饲料中总AA的差值计算ND-和ad -可溶性AA (NDSAA、ADSAA)的浓度(g/kg干物质)。为了估计总AA和整个数据集(n = 74)的体内pcdAA浓度,NDSAA或ADSAA浓度与体内pcdAA之间建立了线性关系:y = 0.823(标准误差[SE] 0.018) x + 10.52 (SE 4.420),其中y表示体内pcdAA (g/kg干物质),x表示NDSAA(谷物)或ADSAA(蛋白质饲料)值(g/kg干物质)。该方程的决定系数(R²)为0.968,17个AA的决定系数范围为0.895 ~ 0.984。本研究表明,体内pcdAA值可以根据先前为pcdCP建立的相同的标准化和快速实验室程序进行估计,基于化学分析,即测定NDIAA和ADIAA,由此计算NDSAA和ADSAA值。
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引用次数: 0
Frequency of the beta-carotene oxygenase 2 (BCO2) allele associated with the yellow fat phenotype in rabbits: insights into the spread of a genetic alteration in a wide variety of breeds and populations 与兔黄脂肪表型相关的β -胡萝卜素加氧酶2 (BCO2)等位基因的频率:对各种品种和种群中基因改变传播的见解
IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-10-14 DOI: 10.1016/j.livsci.2025.105842
Valeria Taurisano , Anisa Ribani , Samuele Bovo , Giuseppina Schiavo , Francesca Bertolini , Michele Schiavitto , Luca Fontanesi
In various animal species, the accumulation of carotenoids that are not completely metabolized leads to yellow fat pigmentation. In rabbits, the yellow-fat phenotype was described by classic genetic studies at the beginning of the last century to be determined by a simple Mendelian recessive allele at the Yellow locus, in linkage with the Albino coat colour locus. More recent studies reported that a deletion of three nucleotides at codon 248 of the beta-carotene oxygenase 2 (BCO2) gene (delAAT) is the causative mutation for the yellow-fat defect when in a homozygous state. In this study, we genotyped the BCO2 polymorphic site in 1041 rabbits from 41 breeds and populations, including albino rabbit breeds. Considering the overall genotyped population, the frequency of the delAAT allele was 11.96%. Homozygous delAAT/delAAT rabbits had a frequency of 3.55%, while the heterozygous rabbits had a frequency of 16.81%. In 12 out of 41 breeds/populations, all rabbits analysed were homozygous for the wild-type allele, while in 29 the mutated allele segregates. Only one breed, Lynx (with a pale silver-blue coat colour and yellow-red shades), was fixed for the delAAT allele, suggesting its potential functional role in determining the yellow-red secondary tint that characterises this breed. Comparing the molecular genetic results with the genetic information reported in earlier literature, the frequency of the mutated allele decreased over the last 80 years, probably due to culling of the carriers for the negative effect on carcass quality, due to the preference of consumers for white fat in rabbit meat.
在各种动物物种中,未完全代谢的类胡萝卜素的积累导致黄色脂肪色素沉着。在兔子中,黄脂表型在上世纪初的经典遗传研究中被描述为由黄色基因座上的一个简单的孟德尔隐性等位基因决定,与白化病毛色基因座相连。最近的研究报道,在纯合子状态下,β -胡萝卜素加氧酶2 (BCO2)基因(delAAT)密码子248处的三个核苷酸缺失是导致黄脂缺陷的致病突变。在这项研究中,我们对来自41个品种和群体的1041只兔子(包括白化兔品种)的BCO2多态性位点进行了基因分型。考虑到整个基因型群体,delAAT等位基因的频率为11.96%。纯合子delAAT/delAAT兔的频率为3.55%,杂合子delAAT/delAAT兔的频率为16.81%。在所分析的41个品种/群体中,有12个品种/群体的野生型等位基因均为纯合子,而29个品种的突变等位基因分离。只有一个品种,猞猁(具有浅银蓝色和黄红色色调),被固定为delAAT等位基因,这表明它在决定该品种特征的黄红色次要色调方面的潜在功能作用。将分子遗传学结果与早期文献报道的遗传信息进行比较,发现突变等位基因的频率在过去80年中有所下降,这可能是由于消费者对兔肉中白色脂肪的偏好导致携带者被扑杀,从而对胴体质量产生负面影响。
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引用次数: 0
Review: Strategies to stimulate piglet creep feed intake before weaning 综述:断奶前刺激仔猪蠕变采食量的策略
IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-10-14 DOI: 10.1016/j.livsci.2025.105840
Malene Hald , Thomas S. Bruun , Trine F. Pedersen , Mette S. Hedemann
Stimulating creep feed intake in piglets is increasingly important due to larger litter sizes, which raise piglet requirements to levels above the sow milk production. Although intake is typically low during the initial weeks, piglets show exploratory behavior (nosing, rooting, and chewing) from the first week of life. Supporting this behavior in conventional farrowing environments can promote early feed engagement. This review highlights strategies to enhance creep feed intake, including housing and creep feeder design, feeding practices, feed characteristics, and management. Creep feeder design and sow confinement (crated vs. loose) affect piglet interaction with feed. Introducing solid feed between 5 and 10 days after farrowing is recommended. The physical form of creep feed (e.g., meal, pellets, crumbles, thermally processed, or liquid) plays a key role. Pellet diameter is especially important; larger pellets (≥ 8 mm) stimulate feeding behavior. Sensory properties (taste, smell, texture) are less critical in attracting piglets. While dietary complexity can influence intake, high-complexity diets do not always outperform low complexity diets, even though these may contain less ingredients and less refined ingredients. Flavor additives, either in the sow’s diet or the creep feed, may improve palatability and familiarity. Increasing the weaning age is the most efficient tool to increase creep feed intake and reduce the proportion of non-eaters at weaning. In conclusion, a multifactorial approach integrating these elements may be most effective in optimizing early feed consumption and adapting to solid feed before weaning.
刺激仔猪的蠕变采食量越来越重要,因为产仔量增加,仔猪的需要量高于母猪的产奶量。虽然最初几周的摄取量通常很低,但仔猪从出生第一周开始就表现出探索性行为(用鼻子嗅、刨根和咀嚼)。在传统的分娩环境中支持这种行为可以促进早期的喂养参与。这篇综述强调了提高蠕变采食量的策略,包括壳体和蠕变喂料器的设计、饲养方法、饲料特性和管理。蠕变喂食器设计和母猪禁闭(板条箱或松散)影响仔猪与饲料的相互作用。建议在分娩后5 ~ 10天引入固体饲料。蠕变饲料的物理形式(例如,粗粉、颗粒、碎屑、热处理或液体)起着关键作用。颗粒直径尤其重要;较大的颗粒(≥8mm)刺激摄食行为。感官特性(味道、气味、质地)在吸引仔猪方面不太重要。虽然饮食的复杂性会影响摄入量,但高复杂性饮食并不总是优于低复杂性饮食,即使这些饮食可能含有更少的成分和更少的精制成分。风味添加剂,无论是在母猪日粮或蠕变饲料,可以提高适口性和熟悉度。提高断奶日龄是提高蠕变采食量,降低断奶期不食猪比例的最有效手段。综上所述,综合这些因素的多因素方法可能是优化早期饲料消耗和断奶前适应固体饲料的最有效方法。
{"title":"Review: Strategies to stimulate piglet creep feed intake before weaning","authors":"Malene Hald ,&nbsp;Thomas S. Bruun ,&nbsp;Trine F. Pedersen ,&nbsp;Mette S. Hedemann","doi":"10.1016/j.livsci.2025.105840","DOIUrl":"10.1016/j.livsci.2025.105840","url":null,"abstract":"<div><div>Stimulating creep feed intake in piglets is increasingly important due to larger litter sizes, which raise piglet requirements to levels above the sow milk production. Although intake is typically low during the initial weeks, piglets show exploratory behavior (nosing, rooting, and chewing) from the first week of life. Supporting this behavior in conventional farrowing environments can promote early feed engagement. This review highlights strategies to enhance creep feed intake, including housing and creep feeder design, feeding practices, feed characteristics, and management. Creep feeder design and sow confinement (crated vs. loose) affect piglet interaction with feed. Introducing solid feed between 5 and 10 days after farrowing is recommended. The physical form of creep feed (e.g., meal, pellets, crumbles, thermally processed, or liquid) plays a key role. Pellet diameter is especially important; larger pellets (≥ 8 mm) stimulate feeding behavior. Sensory properties (taste, smell, texture) are less critical in attracting piglets. While dietary complexity can influence intake, high-complexity diets do not always outperform low complexity diets, even though these may contain less ingredients and less refined ingredients. Flavor additives, either in the sow’s diet or the creep feed, may improve palatability and familiarity. Increasing the weaning age is the most efficient tool to increase creep feed intake and reduce the proportion of non-eaters at weaning. In conclusion, a multifactorial approach integrating these elements may be most effective in optimizing early feed consumption and adapting to solid feed before weaning.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"302 ","pages":"Article 105840"},"PeriodicalIF":1.9,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145363932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic parameters and genomic predictions for economically important traits in Montana® composite cattle using different models 使用不同模型对蒙大拿复合牛经济上重要性状的遗传参数和基因组预测
IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-10-14 DOI: 10.1016/j.livsci.2025.105839
Flávia Cristina Bis , Eduarda da Silva Oliveira , Caroline Assis Almeida , Elisângela Chicaroni de Mattos Oliveira , Letícia Pereira , Rafael Espigolan , Joanir Pereira Eler , Luís Telo Da Gama , Rafael Nuñez Dominguez , José Bento Sterman Ferraz , Fernando Baldi
This study assessed the impact of breed effects, heterosis, and recombination on genomic predictions for growth, reproduction, and body composition traits in Montana® cattle. The database included 124,547 records for Birth Weight (BW), 111,103 for Weaning Weight (WW), 87,740 for Weight at 12 months (W12), 49,249 for Post-weaning Weight Gain (WG), 87,740 for Scrotal Circumference (SC), and 44,873 for Muscularity (MUSC), with 3911 genotyped animals from the Montana® composite program. Models M1 to M5 included fixed effects of contemporary group, embryo transfer, and cow age at calving (linear and quadratic). The effects of direct and maternal breed composition, heterozygosity, and recombination varied across models. From model M2 onward, covariates for biological type, heterosis (direct, maternal, specific), and recombination (direct, maternal, specific) were added. All genomic analyses used the ssGBLUP method, and the LR (Linear Regression) validation method was used to assess predictive ability and model effect influence. Heritability estimates ranged from 0.19 to 0.22 for WW, 0.15 to 0.20 for WG, 0.36 to 0.37 for BW, 0.23 to 0.29 for W12, 0.28 to 0.29 for SC, and 0.17 to 0.19 for MUSC. The most parameterized models showed the best fit by AIC, with M5 best for WW, W12, WG, and SC; M4 for BW; and M3 for MUSC. Model M1 showed the best prediction ability for WW and W12, with the highest accuracies (0.407 and 0.456), best dispersions (1.01 and 0.897), and lowest biases (0.098 and 0.068), respectively. For WG, M1 had the highest accuracy (0.452), M5 the best dispersion (0.940), and M4 the lowest bias (0.028). For BW, M5 showed the highest accuracy (0.452), M4 the best dispersion (1.001), and M3 the lowest bias (-0.006). For SC, M1 had the highest accuracy (0.501), M3 the best dispersion (1.004), and M4 the lowest bias (0.092). For MUSC, M4 had the highest accuracy (0.415) and lowest bias (0.057), while M2 showed the best dispersion (0.979). More parameterized models provided a better fit for variance component estimation. In general, genomic predictions with M1 displayed the highest accuracies for WW, W12, WG, and SC, and lower bias for most traits.
本研究评估了品种效应、杂种优势和重组对蒙大拿牛生长、繁殖和身体组成性状的基因组预测的影响。该数据库包括出生体重(BW) 124,547条记录,断奶体重(WW) 111,103条记录,12个月体重(W12) 87,740条记录,断奶后增重(WG) 49,249条记录,阴囊围(SC) 87,740条记录,肌肉量(MUSC) 44,873条记录,其中3911只基因型动物来自蒙大拿州复合项目。模型M1 ~ M5包括当代组、胚胎移植和产犊年龄的固定效应(线性和二次)。直系和母系品种组成、杂合度和重组的影响在不同的模型中有所不同。从模型M2开始,加入了生物类型、杂种优势(直接、母系、特异性)和重组(直接、母系、特异性)的协变量。所有基因组分析均采用ssGBLUP方法,并采用LR(线性回归)验证方法评估预测能力和模型效应影响。WW的遗传力估计范围为0.19 ~ 0.22,WG为0.15 ~ 0.20,BW为0.36 ~ 0.37,W12为0.23 ~ 0.29,SC为0.28 ~ 0.29,MUSC为0.17 ~ 0.19。AIC参数化模型拟合效果最佳,WW、W12、WG和SC的拟合效果为M5;M4为BW;M3为MUSC。M1模型对WW和W12的预测能力最好,准确率最高(0.407和0.456),离散度最佳(1.01和0.897),偏差最低(0.098和0.068)。对于WG, M1的准确率最高(0.452),M5的分散度最好(0.940),M4的偏差最低(0.028)。对于体重,M5的准确度最高(0.452),M4的离散度最好(1.001),M3的偏差最低(-0.006)。对于SC, M1的准确度最高(0.501),M3的离散度最好(1.004),M4的偏差最低(0.092)。对于MUSC, M4的准确度最高(0.415),偏差最低(0.057),而M2的分散度最佳(0.979)。更多的参数化模型提供了更好的拟合方差分量估计。一般来说,M1对WW、W12、WG和SC的基因组预测精度最高,对大多数性状的偏倚较低。
{"title":"Genetic parameters and genomic predictions for economically important traits in Montana® composite cattle using different models","authors":"Flávia Cristina Bis ,&nbsp;Eduarda da Silva Oliveira ,&nbsp;Caroline Assis Almeida ,&nbsp;Elisângela Chicaroni de Mattos Oliveira ,&nbsp;Letícia Pereira ,&nbsp;Rafael Espigolan ,&nbsp;Joanir Pereira Eler ,&nbsp;Luís Telo Da Gama ,&nbsp;Rafael Nuñez Dominguez ,&nbsp;José Bento Sterman Ferraz ,&nbsp;Fernando Baldi","doi":"10.1016/j.livsci.2025.105839","DOIUrl":"10.1016/j.livsci.2025.105839","url":null,"abstract":"<div><div>This study assessed the impact of breed effects, heterosis, and recombination on genomic predictions for growth, reproduction, and body composition traits in Montana® cattle. The database included 124,547 records for Birth Weight (BW), 111,103 for Weaning Weight (WW), 87,740 for Weight at 12 months (W12), 49,249 for Post-weaning Weight Gain (WG), 87,740 for Scrotal Circumference (SC), and 44,873 for Muscularity (MUSC), with 3911 genotyped animals from the Montana® composite program. Models M1 to M5 included fixed effects of contemporary group, embryo transfer, and cow age at calving (linear and quadratic). The effects of direct and maternal breed composition, heterozygosity, and recombination varied across models. From model M2 onward, covariates for biological type, heterosis (direct, maternal, specific), and recombination (direct, maternal, specific) were added. All genomic analyses used the ssGBLUP method, and the LR (Linear Regression) validation method was used to assess predictive ability and model effect influence. Heritability estimates ranged from 0.19 to 0.22 for WW, 0.15 to 0.20 for WG, 0.36 to 0.37 for BW, 0.23 to 0.29 for W12, 0.28 to 0.29 for SC, and 0.17 to 0.19 for MUSC. The most parameterized models showed the best fit by AIC, with M5 best for WW, W12, WG, and SC; M4 for BW; and M3 for MUSC. Model M1 showed the best prediction ability for WW and W12, with the highest accuracies (0.407 and 0.456), best dispersions (1.01 and 0.897), and lowest biases (0.098 and 0.068), respectively. For WG, M1 had the highest accuracy (0.452), M5 the best dispersion (0.940), and M4 the lowest bias (0.028). For BW, M5 showed the highest accuracy (0.452), M4 the best dispersion (1.001), and M3 the lowest bias (-0.006). For SC, M1 had the highest accuracy (0.501), M3 the best dispersion (1.004), and M4 the lowest bias (0.092). For MUSC, M4 had the highest accuracy (0.415) and lowest bias (0.057), while M2 showed the best dispersion (0.979). More parameterized models provided a better fit for variance component estimation. In general, genomic predictions with M1 displayed the highest accuracies for WW, W12, WG, and SC, and lower bias for most traits.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"302 ","pages":"Article 105839"},"PeriodicalIF":1.9,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145364637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of pre-weaning nutrition on the epigenetic and transcriptomic regulation of mammary gland development in Holstein heifers 断奶前营养对荷斯坦母牛乳腺发育表观遗传和转录组调控的影响
IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-10-14 DOI: 10.1016/j.livsci.2025.105838
Ewelina Semik-Gurgul , Klaudia Pawlina-Tyszko , Artur Gurgul , Tomasz Szmatoła , Justyna Rybińska , Iwona Furgał-Dzierżuk , Barbara Niwińska
Dairy calves require a balanced diet that ensures adequate nutrient intake to support optimal growth and physiological development, which may subsequently influence future lactational performance and reproductive capacity through favourable epigenetic modifications in body tissues. This study aimed to investigate the impact of pre-weaning nutritional strategies on the epigenome and transcriptome of the mammary gland tissues in Holstein heifers. Twelve Holstein-Friesian heifer calves were included in the experiment. During their first two weeks of life, all calves received a milk replacer (MR) that met their nutritional requirements, along with unlimited access to concentrate feed and water. At 14 ± 1 days of age, the calves were randomly assigned to one of two feeding treatments. The applied diets involved restricted milk replacer intake (R, n = 6) and ad libitum milk replacer intake (AL, n = 6). Feeding treatments lasted for 48 days, after which all calves were euthanised at 62 ± 1 days of age. Mammary parenchyma (PAR) and mammary fat pad (MFP) tissues were collected and analysed using RRBS, miRNA-seq, and RNA-seq approaches. When comparing two diets, 268 differentially methylated sites (DMSs) were identified in the PAR, and 288 DMSs were found in MFP tissue. The obtained data allowed identification of four differentially expressed miRNAs (DE miRNAs) in PAR and 35 DE miRNAs in MPF tissue. Furthermore, transcriptomic analysis revealed that mammary fat pad tissue reacted to varying diets with a higher number of differentially expressed genes, indicating that parenchyma tissue is less responsive to nutritional changes. Based on integrative omics analysis, specific genes were identified in MFP tissue, whose expression levels were correlated with the level of CpG methylation and miRNA expression, revealing enriched biological processes. However, several important limitations should be considered when interpreting these results, including the relatively small sample size and the absence of long-term follow-up. These findings suggest that pre-weaning feeding strategies have a significant influence on the epigenetic regulation of gene expression, potentially determining the future lactation potential of dairy heifers.
奶牛需要均衡的饮食,以确保足够的营养摄入,以支持最佳的生长和生理发育,这可能随后通过有利的身体组织表观遗传修饰影响未来的泌乳性能和繁殖能力。本研究旨在探讨断奶前营养策略对荷斯坦小母牛乳腺组织表观基因组和转录组的影响。试验选用12头荷斯坦-弗里西亚小母牛犊牛。在出生后的前两周,所有小牛都接受了符合其营养需求的代乳剂(MR),并无限制地获得浓缩饲料和水。在14±1日龄时,将犊牛随机分为两组饲喂。应用日粮包括限制代乳品摄入量(R, n = 6)和随意代乳品摄入量(AL, n = 6)。饲喂48 d,于62±1日龄安乐死。收集乳腺实质组织(PAR)和乳腺脂肪垫组织(MFP),采用RRBS、miRNA-seq和RNA-seq方法进行分析。对比两种饲料,在PAR中鉴定出268个差异甲基化位点(dms),在MFP组织中发现288个差异甲基化位点。获得的数据可以鉴定PAR组织中的4个差异表达mirna (DE mirna)和MPF组织中的35个差异表达mirna。此外,转录组学分析显示,乳腺脂肪垫组织对不同的饮食有更多的差异表达基因,这表明薄壁组织对营养变化的反应较小。基于整合组学分析,在MFP组织中鉴定出特异性基因,其表达水平与CpG甲基化水平和miRNA表达水平相关,揭示了丰富的生物学过程。然而,在解释这些结果时应考虑几个重要的局限性,包括相对较小的样本量和缺乏长期随访。这些结果表明,断奶前饲养策略对基因表达的表观遗传调控有显著影响,可能决定奶牛未来的泌乳潜力。
{"title":"Effect of pre-weaning nutrition on the epigenetic and transcriptomic regulation of mammary gland development in Holstein heifers","authors":"Ewelina Semik-Gurgul ,&nbsp;Klaudia Pawlina-Tyszko ,&nbsp;Artur Gurgul ,&nbsp;Tomasz Szmatoła ,&nbsp;Justyna Rybińska ,&nbsp;Iwona Furgał-Dzierżuk ,&nbsp;Barbara Niwińska","doi":"10.1016/j.livsci.2025.105838","DOIUrl":"10.1016/j.livsci.2025.105838","url":null,"abstract":"<div><div>Dairy calves require a balanced diet that ensures adequate nutrient intake to support optimal growth and physiological development, which may subsequently influence future lactational performance and reproductive capacity through favourable epigenetic modifications in body tissues. This study aimed to investigate the impact of pre-weaning nutritional strategies on the epigenome and transcriptome of the mammary gland tissues in Holstein heifers. Twelve Holstein-Friesian heifer calves were included in the experiment. During their first two weeks of life, all calves received a milk replacer (MR) that met their nutritional requirements, along with unlimited access to concentrate feed and water. At 14 ± 1 days of age, the calves were randomly assigned to one of two feeding treatments. The applied diets involved restricted milk replacer intake (R, <em>n</em> = 6) and ad libitum milk replacer intake (AL, <em>n</em> = 6). Feeding treatments lasted for 48 days, after which all calves were euthanised at 62 ± 1 days of age. Mammary parenchyma (PAR) and mammary fat pad (MFP) tissues were collected and analysed using RRBS, miRNA-seq, and RNA-seq approaches. When comparing two diets, 268 differentially methylated sites (DMSs) were identified in the PAR, and 288 DMSs were found in MFP tissue. The obtained data allowed identification of four differentially expressed miRNAs (DE miRNAs) in PAR and 35 DE miRNAs in MPF tissue. Furthermore, transcriptomic analysis revealed that mammary fat pad tissue reacted to varying diets with a higher number of differentially expressed genes, indicating that parenchyma tissue is less responsive to nutritional changes. Based on integrative omics analysis, specific genes were identified in MFP tissue, whose expression levels were correlated with the level of CpG methylation and miRNA expression, revealing enriched biological processes. However, several important limitations should be considered when interpreting these results, including the relatively small sample size and the absence of long-term follow-up. These findings suggest that pre-weaning feeding strategies have a significant influence on the epigenetic regulation of gene expression, potentially determining the future lactation potential of dairy heifers.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"302 ","pages":"Article 105838"},"PeriodicalIF":1.9,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145321710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A diet containing Cyberlindnera jadinii yeast as a protein source demonstrates comparable benefits to a formic acid and probiotic supplemented diet in post-weaning piglets 在断奶后仔猪中,以jadinii Cyberlindnera酵母为蛋白质来源的饲粮与添加甲酸和益生菌的饲粮具有相当的益处
IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-10-12 DOI: 10.1016/j.livsci.2025.105835
Özgün C Onarman Umu , Henning Sørum , Anne Stine Ekker , Peng Lei , Purushothaman Kathiresan , Charles McLean Press , Kari Ljøkjel , Liv Torunn Mydland , Nils Petter Kjos , Margareth Øverland
The increasing demand for sustainable protein sources in livestock production necessitates the evaluation of novel feed ingredients that can reduce environmental impact while maintaining animal health and performance. The effects of replacing soybean meal with Cyberlindnera jadinii, a locally produced microbial yeast protein, in the diet of post-weaning piglets under commercial production conditions were analyzed. A total of 840 piglets were enrolled in a field experiment conducted over 3 consecutive production periods. Piglets were allocated to 2 dietary treatments: a control diet containing soybean meal, formic acid products and a probiotic, and an experimental diet where C. jadinii yeast replaced 45% of the dietary protein. Growth performance, fecal consistency, and gut microbiota composition were evaluated over a 4 wk post-weaning period. Piglets fed the yeast-based diet exhibited an overall lower average daily gain (ADG; P < 0.01) and average daily feed intake (ADFI; P < 0.01) compared to those on the control diet, while feed conversion ratio (FCR) remained similar between groups. No differences in fecal consistency or clinical signs of diarrhea were observed, indicating maintained gut health despite reduced feed intake. Microbiota profiling revealed dietary effects on microbial diversity and composition in both the ileum and colon. Notably, the yeast-fed piglets showed greater relative abundances of Lactobacillus johnsonii, Streptococcus gallolyticus, and members of the Prevotellaceae family, while the control-fed piglets had greater abundances of Lactobacillus amylovorus and Blautia hansenii. These findings indicate that C. jadinii yeast can partially replace soybean meal and formic acid in weaner pig diets without compromising health status, although some reduction in growth performance was observed. The altered gut microbiota, including enrichment of beneficial taxa such as L. johnsonii, suggests functional modulation of gut health, supporting the potential of yeast-based proteins as a sustainable feed strategy in pig production systems.
畜牧业生产对可持续蛋白质来源的需求日益增加,因此必须评估能够在保持动物健康和生产性能的同时减少对环境影响的新型饲料成分。在商业化生产条件下,研究了用本地生产的微生物酵母蛋白Cyberlindnera jadinii代替豆粕对断奶仔猪日粮的影响。共有840头仔猪参加了连续3个生产期的田间试验。将仔猪分为2种饲粮处理:对照组饲粮中添加豆粕、甲酸产品和益生菌,试验组饲粮中添加贾氏酵母替代45%的饲粮蛋白质。在断奶后4周内评估生长性能、粪便稠度和肠道微生物群组成。总体而言,酵母基础饲粮的平均日增重(ADG; P < 0.01)和平均日采食量(ADFI; P < 0.01)低于对照组,但各组间饲料系数(FCR)基本一致。粪便稠度和腹泻临床症状均无差异,表明尽管采食量减少,但肠道健康仍保持正常。微生物群分析揭示了饮食对回肠和结肠微生物多样性和组成的影响。值得注意的是,酵母喂养的仔猪具有较高的约氏乳杆菌、溶食链球菌和普氏菌科成员的相对丰度,而对照喂养的仔猪具有较高的淀粉样乳杆菌和汉斯蓝杆菌的相对丰度。上述结果表明,jadinii酵母可以部分替代断奶猪饲粮中的豆粕和甲酸,而不会影响仔猪的健康状况,但会降低仔猪的生长性能。肠道微生物群的改变,包括有益类群如约氏乳杆菌的富集,表明肠道健康的功能调节,支持酵母蛋白作为猪生产系统中可持续饲料策略的潜力。
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引用次数: 0
Effect of heat stress and feed restriction on performance, carcass traits, and meat quality of growing rabbits 热应激和限饲对生长兔生产性能、胴体性状和肉品质的影响
IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-10-11 DOI: 10.1016/j.livsci.2025.105836
Emanuele Pontalti , Marco Cullere , Zsolt Szendrő , Zsolt Matics , Zsolt Gerencsér , Bianca Palumbo , Antonella Dalle Zotte
The effects of heat stress and feed restriction were evaluated on a total of 180 weaned rabbits divided into three experimental groups (60 animals/group): 2 groups were fed ad libitum and reared under different temperatures (20 °C – 20AD and 30 °C – 30AD), while a third group was housed under controlled temperature (20 °C) but pair-fed to 30AD rabbits, thus feed restricted (20FR). During the trial, both 30AD and 20FR groups exhibited reduced growth performance, including body weight and daily weight gain (both, P < 0.001), although feed conversion ratio improved (P = 0.016). The reference carcasses of 20FR and 30AD rabbits were lighter and leaner (both, P < 0.001) than that of 20AD rabbits, while the slaughter yield decreased only in 20FR rabbits (P = 0.001). Regarding meat physical traits, 20FR rabbits exhibited the highest pHu (P < 0.001) and the lowest total losses (P < 0.001), whereas the meat-to-bone ratio decreased in both 20FR and 30AD groups (P = 0.007). As for meat proximate composition, protein and lipid contents were lower (P = 0.008 and P = 0.0002, respectively) in 20FR and 30AD rabbits, while water content was greater (P < 0.001) compared to 20AD rabbits. At the lipid level, higher TBARS (P = 0.001) were found in both 20FR and 30AD groups. The 20FR and 30AD groups showed some differences in their carcass and meat quality traits, however the majority of changes induced by chronic heat stress were mostly attributed to the reduced feed intake.
研究热应激和限饲对180只断奶家兔的影响,将其分为3个实验组(每组60只):2组自由饲养,在不同温度(20℃- 20 ad和30℃- 30 ad)下饲养,第三组在控制温度(20℃)下饲养,对30 ad的家兔进行配对饲养,即限饲(20 fr)。试验期间,30AD和20FR组的生长性能(包括体重和日增重)均有所降低(P < 0.001),但饲料系数有所提高(P = 0.016)。20FR和30AD家兔的参考胴体比20AD家兔更轻、更瘦(均P <; 0.001),而只有20FR家兔的屠宰产量下降(P = 0.001)。在肉质性状方面,20FR组的pHu最高(P < 0.001),总损失最低(P < 0.001),而肉骨比在20FR组和30AD组均有所降低(P = 0.007)。肉质成分方面,20FR和30AD兔的蛋白质和脂肪含量低于20AD兔(P = 0.008和P = 0.0002),水分含量高于20AD兔(P < 0.001)。在脂质水平上,20FR组和30AD组的TBARS均较高(P = 0.001)。20FR和30AD组胴体和肉质性状有一定差异,但慢性热应激引起的变化主要是采食量的减少。
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引用次数: 0
Refining Irish breeding goals for sustainable suckler systems through the incorporation of an enteric methane emissions trait 通过纳入肠道甲烷排放特性,为可持续的哺乳系统改进爱尔兰育种目标
IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-10-11 DOI: 10.1016/j.livsci.2025.105837
Clodagh V. Ryan , Deirdre C. Purfield , David Kenny , Paul Crosson , Ross D. Evans
Genetic selection presents a long-term opportunity to reduce enteric methane emissions in beef cattle. This study evaluated the effect of incorporating an enteric methane production trait into the Irish Terminal Index, an index that is typical of global terminal-type indexes and quantified its impact on methane output and other economically important traits. At present, the Irish Terminal Index incorporates a Carbon sub-index based on life cycle assessment in an effort to reduce farm carbon emissions. Selection index scenarios were modelled to reflect an index with no environmental consideration, the current status quo, the inclusion of a direct enteric methane trait and finally the impact of changes in carbon pricing. Genetic parameters were derived from a national multi-breed dataset of 1508 beef animals with enteric methane phenotypes. Selection based on the current index which includes a lifecycle carbon weighting, increased daily enteric methane emissions by up to 2.14 g/day, while the inclusion of a direct enteric methane trait reversed the response, with predicted reductions of 0.22g/day and 4.20 g/day depending on carbon price. Associated changes included a 13.81 kg increase in carcass weight response and a 0.33 kg reduction in feed intake per animal under the highest environmental weighting (Carbon valued at €160/tonne and a direct enteric methane trait). Scaled to the national herd, this would equate to an annual reduction of approximately 27,400 tonnes of carbon dioxide equivalent and an opportunity cost of 1311 tonnes of carcass output. Selection direction remained favourable across all production traits, with minimal impact on calving, docility, and conformation traits. These results demonstrate that enteric methane can be incorporated into the national breeding goal with measurable reductions in emissions and limited trade-offs in economically relevant performance traits.
基因选择提供了减少肉牛肠道甲烷排放的长期机会。本研究评估了将肠道甲烷产量性状纳入爱尔兰终端指数(Irish Terminal Index)的效果,并量化了其对甲烷产量和其他经济上重要性状的影响。爱尔兰终端指数是全球终端类型指数的典型指标。目前,爱尔兰码头指数纳入了基于生命周期评估的碳子指数,以减少农场碳排放。对选择指数情景进行建模,以反映不考虑环境因素、现状、包含直接肠道甲烷特征以及碳定价变化影响的指数。遗传参数来源于1508只肠道甲烷表型牛肉动物的全国多品种数据集。基于当前指标(包括生命周期碳权重)的选择,每日肠道甲烷排放量增加了2.14 g/天,而直接肠道甲烷特征的纳入逆转了这一反应,根据碳价格的不同,预计减少0.22g/天和4.20 g/天。相关的变化包括在最高环境加权(碳价值为160欧元/吨和直接肠道甲烷特征)下,胴体体重反应增加13.81公斤,每头动物的采食量减少0.33公斤。按全国牛群的比例计算,这将相当于每年减少约27400吨二氧化碳当量,并减少1311吨胴体产量的机会成本。选择方向在所有生产性状中保持有利,对产犊、温顺和构象性状的影响最小。这些结果表明,肠道甲烷可以被纳入国家养殖目标,减少可测量的排放,并限制经济相关性能性状的权衡。
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引用次数: 0
Calf presence in the cow herd: Associations with growth, health, and herd horn status in a cow-calf contact system 牛群中小牛的存在:在牛-小牛接触系统中与生长、健康和牛角状态的关系
IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2025-10-09 DOI: 10.1016/j.livsci.2025.105834
Romane Gillet , Marie Schneider , Kerstin Barth
Prolonged cow-calf contact in dairy farming is receiving considerable attention. However, research on systems that allow calves to access the whole dairy herd has been limited. This study investigated the time that German Holstein calves spent daily within the dairy herd of their dams, its association with calf growth and health, and the effect of herd (horned vs. polled). The calves with whole-day contact (WDC, n= 23) could enter the herd at any time during the day, except during milking; the calves with daytime contact (DTC, n= 26) had only access between morning and evening milking. Calves with no herd contact (NOC, n= 42) served as a control. Calves were weighed weekly and their health status was assessed. Between the 2nd and 11th weeks of life, the time spent in the cow herd decreased in WDC calves (h d-1, horned WDC: - 10.9, polled WDC: - 3.7, P< 0.001), but did not differ in DTC calves (P> 0.1). More time in the herd was associated with higher growth rates (P= 0.003). However, no effect of the herd was observed on calf growth or health. WDC calves had the highest growth rate compared to DTC and NOC (g d-1, 991 ± 36, 718 ± 35, 869 ± 28, respectively, P< 0.05). Unrestricted access to the cow herd resulted in individual variation in the time calves spent within the herd, with no observed effects on growth or health.
在奶牛养殖中,奶牛与小牛的长期接触正受到相当大的关注。然而,关于允许小牛进入整个奶牛群的系统的研究一直很有限。本研究调查了德国荷斯坦小牛每天在其水坝的奶牛群中度过的时间,其与小牛生长和健康的关系,以及牛群的影响(有角vs有轮)。全天接触的小牛(WDC, n= 23)除挤奶期间外,全天任何时间均可进入牛群中;白天接触的小牛(DTC, n= 26)仅在早晚挤奶之间接触。与牛群没有接触的小牛(NOC, n= 42)作为对照。小牛每周称重并评估其健康状况。在第2周至第11周之间,WDC犊牛在牛群中度过的时间减少了(h -1,有角WDC: - 10.9,有轮WDC: - 3.7, P< 0.001),但DTC犊牛没有差异(P> 0.1)。在畜群中待的时间越长,生长速度越快(P= 0.003)。然而,没有观察到畜群对小牛生长或健康的影响。与DTC和NOC相比,WDC犊牛的生长率最高(g d-1、991±36、718±35、869±28,p < 0.05)。不受限制地进入牛群导致小牛在牛群中度过的时间存在个体差异,未观察到对生长或健康的影响。
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引用次数: 0
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Livestock Science
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