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Gas chromatography analysis of the microwave-aided extracted agarwood oil from physically induced Aquilaria malaccensis trees in Northern Thailand 泰北地区物理诱导沉香树微波萃取沉香油的气相色谱分析
Pub Date : 2022-12-31 DOI: 10.54279/mijeec.v4i3.248277
Chuyên Thuận Nguyễn, Chanthana Susawaengsup, Thomas Singer, Krittiya Tongkoom, Ralf Oberg, Rapeephun Dangtungee, P. Bhuyar
Agarwood (Aquilaria malaccensis) oil is one of the essential bioactive materials in the scent/ perfume industry for the middle east and gulf countries. The built-in functionality and availability of bioactive components of oil make agarwood more precious. In this study, the physically induced infected agarwood tree chops were extracted by microwave assisted Soxhlet extraction. Moreover, the extracted oil samples were analyzed against control not infected trees using Gas chromatography-mass spectroscopy (GC-MS) by GCMS (Agilent GC 6890zN MS 5973). The microwave assisted Soxhlet extraction revealed a higher amount of oil extraction. In the physically induced samples, 24 signal peaks were recorded employing GC-MS. The most prominent peak area recorded was -5-hydroxy-3-methyl-1-indanone detected at minute 41.89 with a peak area of 17.64%, followed by -methyl 4,4,7-trimethyl 4.7-dihydro indan -6- carboxylate at 23.17 min, occupying an area of 15.54%; followed by 3-carene recorded at 46.61 minutes with an area. According to this research, the microwave-assisted Soxhlet extraction method outperformed and can be utilized in the agarwood industry for enhanced oil production.
沉香油是中东和海湾国家香水工业中必不可少的生物活性物质之一。内置的功能和可用的生物活性成分的油使沉香更加珍贵。本研究采用微波辅助索氏提取法提取物理诱导的沉香木屑。采用气相色谱-质谱联用(GCMS) (Agilent GC 6890zN MS 5973)对提取的油样与未感染的对照进行分析。微波辅助索氏提取法可获得较高的油提取率。在物理诱导的样品中,用GC-MS记录了24个信号峰。记录到的峰面积最突出的是-5-羟基-3-甲基-1-吲哚酮,在41.89 min检测到,峰面积为17.64%,其次是-甲基4,4,7-三甲基4.7-二氢吲哚-6-羧酸酯,在23.17 min检测到,峰面积为15.54%;紧随其后的是3-carene,用时46.61分钟。研究结果表明,微波辅助索氏提取法在沉香工业中具有较好的优势,可用于提高沉香油的产量。
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引用次数: 1
Application of hurdle Poisson model to predict the abundance of toxic cyanobacteria Microcystis in reservoirs 应用障碍泊松模型预测储层中有毒微囊藻的丰度
Pub Date : 2022-12-17 DOI: 10.54279/mijeec.v4i3.247529
Truc-Ly Le-Huynh, T. Itayama, Kaito Mitsunaga, Misigo W. S. Angalika, Seiji Suzuki
The blooming of toxic cyanobacteria Microcystis in eutrophicated reservoirs causes serious difficulties for water supply worldwide. For the appropriate management of such reservoirs, a prediction model of toxic cyanobacteria Microcystis can be a useful tool. Therefore, this study aims to develop a Bayesian hurdle Poisson model for statistical prediction of toxic Microcystis from only two predictors, air temperature and trophic state index (TSI) calculated from chlorophyll-a. The gene copy number of the mcyB gene was used as a surrogate of toxic Microcystis cell density. The data on mcyB gene and chlorophyll-a were collected from 20 reservoirs in Nagasaki Prefecture (Japan). The daily average air temperature was downloaded from the local meteorological stations and a mean for 30 days before sampling date was calculated. The results showed that higher temperature and larger TSI accelerate the growth of toxic Microcystis. Furthermore, this model successfully predicted mcyB gene copy number as a surrogate of toxic Microcystis cell density for different conditions of air temperature and TSI with sufficient accuracy. Therefore, the proposed model has the potential to be a useful prediction tool for toxic cyanobacteria Microcystis in the integrated management of reservoirs.
富营养化水库中有毒微囊藻蓝藻的大量繁殖给世界范围内的供水造成了严重的困难。对于这样的水库的适当管理,有毒蓝藻微囊藻的预测模型可以是一个有用的工具。因此,本研究旨在建立一个贝叶斯障碍泊松模型,仅从气温和叶绿素-a计算的营养状态指数(TSI)两个预测因子进行毒性微囊藻的统计预测。用mcyB基因拷贝数作为毒性微囊藻细胞密度的替代指标。对日本长崎县20个水库的mcyB基因和叶绿素-a进行了分析。从当地气象站下载每日平均气温,计算采样日前30天的平均值。结果表明,较高的温度和较大的TSI有利于毒性微囊藻的生长。此外,该模型成功预测了mcyB基因拷贝数作为毒性微囊藻细胞密度在不同空气温度和TSI条件下的替代物,具有足够的准确性。因此,该模型有可能成为水库综合管理中有毒微囊蓝藻的有用预测工具。
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引用次数: 0
Removal of CO2 and H2S from biogas and enhanced compressed bio-methane gas production from swine manure and elephant grass 去除沼气中的CO2和H2S,提高猪粪和象草压缩生物甲烷气体的产量
Pub Date : 2022-12-01 DOI: 10.54279/mijeec.v4i3.248270
N. Dussadee, Kamoldara Reansuwan, Rameshprabu Ramaraj, Yuwalee Unpaprom
Anaerobic co-digestion of concentrated swine manure with elephant grass silage was used, and this study carried out in full-scale three continuously stirred tank reactors was operated at mesophilic temperature. The Biogas impurity removal procedure was enhanced by using a molecular sieve and activated carbon. Two molecular sieve tanks are used to absorb water from the biogas. An activated carbon tank (2 meters) was used to eliminate H2S to < 1 ppm before entering the gas tank. In the form of compressed bio-methane gas (CBG), production was 14,400 m3/day (CH4 60-70%), and the amount of CBG was 9,600 m3/day 6.8 tons/day. The raw biogas containing CH4, CO2, and O2 are 68.8%, 29.7% and 0% with H2S 768 ppm. After the enhancement process, CBG having CH4, CO2, O2 are 89.35%, 10.05 0% and 0.02% with < 0.01 ppm H2S. Therefore, after the purification process amount of CO2, H2S gas was considerably reduced and CH4 was improved by up to 90% by volume, and then the CBG was compressed to 250 bar tanks to the fuel for cars. Therefore, these results clearly demonstrate that the activated carbon method is feasible to process for the removal of CO2 and H2S from biogas in a large-scale plant.
采用浓缩猪粪与象草青贮的厌氧共消化工艺,在中温条件下,在3个连续搅拌槽式反应器中进行了研究。采用分子筛和活性炭对沼气除杂过程进行了强化。两个分子筛罐用于从沼气中吸收水分。在进入储气罐之前,使用一个2米的活性炭罐将H2S滤除至< 1ppm。压缩生物甲烷气(CBG)产量为1.44万m3/d (CH4 60-70%), CBG产量为9600 m3/d(6.8吨/d)。当H2S浓度为768 ppm时,沼气CH4、CO2和O2的含量分别为68.8%、29.7%和0%。在< 0.01 ppm H2S条件下,CBG的CH4、CO2、O2含量分别为89.35%、10.05%和0.02%。因此,经过净化处理后,CO2、H2S气体的体积量大大减少,CH4的体积量提高了90%,然后将CBG压缩到250 bar的罐中作为汽车燃料。因此,这些结果清楚地表明,活性炭法在大型工厂中去除沼气中的CO2和H2S是可行的。
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引用次数: 0
Statistical analysis of the effects of environmental factors and fish species on class-sorted phytoplankton composition in aquaculture ponds in northern Thailand 环境因子和鱼种对泰国北部水产养殖池塘浮游植物分类组成影响的统计分析
Pub Date : 2022-11-15 DOI: 10.54279/mijeec.v4i3.247635
Truc-Ly Le-Huynh, N. Iwami, N. Whangchai, Redel Gutierrez, K. Shimizu, T. Itayama
Understanding the phytoplankton in aquaculture ponds is critical for proper pond management. Despite the importance, the relationships between phytoplankton composition, cultured fish type, season, and nutrients were not well understood. This study statistically investigated these relationships in aquaculture ponds. Data collected at 21 tilapia and 13 catfish ponds in September 2009 (wet season), December 2009 (cold season), and March 2010 (hot season) in northern Thailand were used for the analysis. The statistical analysis showed that PO4-P and NH4-N concentrations in catfish ponds were significantly higher than in tilapia ponds (p < 0.05, Wilcoxon test). The cyanobacterial abundance in catfish ponds was significantly greater than in tilapia ponds (p < 0.05, Wilcoxon test). In the hot season (March), green algae were abundant (p < 0.05), while cyanobacteria were depleted (p < 0.05). Multiple linear regression model was applied to determine important factors for statistically explaining cyanobacterial abundance. The result indicated that the best model selected by AICc included season and pond type as factors influencing cyanobacterial abundance but not nutrients. However, since the effect of nutrients was included in the difference in nutrient concentration due to the difference in fish species in the ponds, it was speculated that nutrients were insignificant as explanatory variables. Furthermore, it was hypothesized that cyanobacterial abundance was reduced in March (hot season) because the predation of cyanobacteria by tilapia may be encouraged at high temperature.
了解水产养殖池塘中的浮游植物对正确的池塘管理至关重要。尽管具有重要意义,但浮游植物组成、养殖鱼类类型、季节和营养物质之间的关系尚未得到很好的理解。本研究对水产养殖池塘的这些关系进行了统计调查。分析使用了2009年9月(雨季)、2009年12月(寒冷季节)和2010年3月(炎热季节)在泰国北部21个罗非鱼和13个鲶鱼池塘收集的数据。统计分析表明,鲶鱼池中PO4-P和NH4-N浓度显著高于罗非鱼池(p < 0.05, Wilcoxon检验)。鲶鱼池蓝藻丰度显著高于罗非鱼池(p < 0.05, Wilcoxon检验)。在炎热季节(3月),绿藻丰富(p < 0.05),蓝藻减少(p < 0.05)。采用多元线性回归模型确定蓝藻丰度统计解释的重要因素。结果表明,AICc选择的最佳模型包括季节和池塘类型作为蓝藻丰度的影响因素,而不包括营养成分。但由于鱼种不同导致的营养物浓度差异中包含了营养物的影响,推测营养物作为解释变量不显著。此外,假设蓝藻丰度在3月(炎热季节)减少,因为罗非鱼在高温下可能会促进蓝藻的捕食。
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引用次数: 0
Bioethanol production from low-grade konjac powder via combination of alkaline and thermal pretreatments 低品位魔芋粉碱预处理与热预处理相结合生产生物乙醇的研究
Pub Date : 2022-11-05 DOI: 10.54279/mijeec.v4i3.248262
Warichsa Kongchan, Yuwalee Unpaprom, Natthawud Dussadee, R. Ramaraj
Combining alkaline and thermal pretreatments for the conversion of low-grade konjac powder to fermentable sugars was studied. After pretreatment, an enzyme hydrolysis procedure was applied with cellulase. The pretreatment and hydrolyzed liquids were used with Saccharomyces cerevisiae for the fermentation process in the batch system.  Analysis with a spectrometer revealed that the fundamental matter of low-quality konjac powder was severely degraded in order to enhance the fermentable sugars. Our investigation found that the alkaline combination thermal pretreatment produced the most reducing sugars. The impacts of several operational factors were examined. The highest alcohol content (3.551g/L), heating value (3570.27Cal/g) and energy values (17.15 MJ/kg) were achieved, this might be a very promising method of low-grade konjac powder use. Pretreatment of low-grade konjac powder for bioethanol production is highly advised due to its extremely low cost and high yield of reducing sugars and bioethanol.
研究了碱预处理和热预处理相结合的低品位魔芋粉转化为可发酵糖的工艺。预处理后,用纤维素酶进行酶解。将预处理液和水解液与酿酒酵母菌一起在间歇系统中进行发酵。光谱仪分析表明,劣质魔芋粉的基本物质被严重降解,以提高可发酵糖的含量。我们的研究发现,碱性复合热预处理产生的还原糖最多。考察了几个操作因素的影响。乙醇含量最高(3.551g/L),热值最高(3570.27Cal/g),能量值最高(17.15 MJ/kg),是一种很有前途的低度魔芋粉利用方法。低品位魔芋粉预处理生产生物乙醇具有成本极低、还原糖和生物乙醇产量高的优点。
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引用次数: 0
Production of biofuel from microalgae grown in wastewater- A review 废水微藻生产生物燃料的研究进展
Pub Date : 2022-10-27 DOI: 10.54279/mijeec.v4i3.248256
K. M. Palanisamy, O. Bhat, M. O. Oteikwu, N. Govindan, G. P. Maniam, Rameshprabhu Ramaraj, Y. Unpaprom
There is an urgent demand for an evolution of renewable energy resources due to the rising global energy demand, resource depletion, growing emphasis on reducing climate change, and resource scarcity. Among many resources, Microalgae are thought to be the cleanest alternative feedstock for biofuels and biorefineries because of their advantages of quick growth, effective carbon dioxide fixation, lack of competition for agricultural production and potable water, and the potential to accumulate massive volumes of lipids and carbohydrates. Therefore, this review has discussed the different wastewater which can support and enhance the growth of microalgae, biosynthesis of lipids, production of biofuels from microalgae, and biochemical properties of biofuel. In order to minimize the accumulation of wastewater to protect the environment from pollution, apply wastewater to grow microalgae to achieve the goal of bio circular economy. This review provides clear details and a summary of the production of biofuel from microalgae grown in wastewater.
由于全球能源需求的增长、资源的枯竭、减少气候变化的日益重视和资源的稀缺,对可再生能源的发展有着迫切的需求。在许多资源中,微藻被认为是生物燃料和生物炼制的最清洁的替代原料,因为它们具有快速生长、有效的二氧化碳固定、在农业生产和饮用水方面缺乏竞争、以及积累大量脂质和碳水化合物的潜力等优点。为此,本文就支持和促进微藻生长的不同废水、生物脂合成、微藻生产生物燃料以及生物燃料的生化特性进行了综述。为了尽量减少废水的积累,保护环境免受污染,利用废水培养微藻,实现生物循环经济的目标。本文综述了利用废水中生长的微藻生产生物燃料的研究进展。
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引用次数: 0
Biomass-derived nano-catalyst for biodiesel production from waste cooking oil 废弃食用油生产生物柴油的生物质衍生纳米催化剂
Pub Date : 2022-10-09 DOI: 10.54279/mijeec.v4i3.248241
Anodar Ratchawet, P. Chaiworn
This study aimed to produce a nanocatalysts from inexpensive barley straw using nickel (Ni) and cobalt (Co) to support waste cooking oil-based biodiesel production. At 400 °C without oxygen and 1-5 bars of pressure, the gasification procedure of barley straw biomass (100g dry basis) was utilized in a muffle furnace with Ni and Co nano-catalysts. The biomass:Ni:Co catalyst mixing ratio is 1:1:1. The catalyst content and reaction time were applied for 2 hours. Then, at molar ratios of methanol:oil (6:1, 9:1, and 12:1) with the amount of catalyst (1, 2, and 3% weight percent basis), at 2 hours reaction time. Accordingly, the factors impacting the transesterification of biodiesel synthesis were evaluated. The process employing methanol:oil molar ratio of 6:1 and a catalyst quantity of 2% wt was the best for producing biodiesel. Based on the results of this study, nanocatalysts formed from biomass, which can be obtained from agricultural waste, hold commercial promise as a catalyst source for biodiesel.
这项研究旨在利用镍(Ni)和钴(Co)从廉价的大麦秸秆中生产纳米催化剂,以支持废食用油为基础的生物柴油生产。在400℃无氧条件下,在1-5 bar压力下,采用Ni和Co纳米催化剂在马弗炉中气化大麦秸秆生物质(100g干基)。生物质:Ni:Co催化剂的混合比例为1:1:1。催化剂用量和反应时间均为2小时。然后,在甲醇:油的摩尔比(6:1,9:1和12:1)和催化剂的量(1,2和3%重量百分比基础)下,反应2小时。在此基础上,对影响生物柴油酯交换反应的因素进行了评价。以甲醇:油摩尔比为6:1,催化剂用量为2% wt制备生物柴油的最佳工艺条件。基于这项研究的结果,可以从农业废弃物中获得的生物质形成的纳米催化剂作为生物柴油的催化剂来源具有商业前景。
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引用次数: 0
Assessing nutrient budget of ungauged catchment using intermittent water quality markers 利用间歇水质标记评估未计量集水区的营养收支
Pub Date : 2022-09-28 DOI: 10.54279/mijeec.v4i3.247534
Misigo W. S. Angalika, Seiji Suzuki, Truc-Ly Le-Huynh, T. Itayama, Wataru Tanaka
This study is a preliminary spatial-temporal assessment method of the ungauged catchment to determine the variation in water quality (WQ) and the land use influence on river basins’ health. The intermittent WQ data, the principal component analysis, and the redundancy analysis were used to evaluate the (dis)similarity among the 10 ungauged streams and their significance in the entire catchment. These revealed some similarities/differences in nutrient pollution and latent land-use influence on the streams’ health. There were similarities between R6-R7, R9-R10, among R1 to R4 basins, while R5 and R8 had distinct variances in their WQ dynamics. The intensive vegetable and rice production in R5, R7, R8, R9, and R10 basins were the major sources of high nutrient concentrations. The unique variations, especially in R5 and R8 basins could be attributed to other different pollution sources. Hence, it’s of great significance to carry out comprehensive research in the above 5 river basins. That is the efficiency of management practices, identification of pollution sources, and the extent to which the elevated nutrients in the streams interact with biota within the river regime. This research offers a method to evaluate WQ dynamics in relation to human interferences in river basins of a catchment with limited data under similar climatic conditions.
本研究为确定流域水质变化和土地利用对流域健康影响的初步时空评价方法。采用断续WQ数据、主成分分析和冗余分析,评价了10条未测量河流之间的(非)相似性及其在整个流域的意义。这些结果揭示了营养物污染和土地利用对河流健康的潜在影响的相似性/差异性。r6 ~ r7、r9 ~ r10、R1 ~ R4盆地间存在相似性,而R5、R8盆地间差异较大。R5、R7、R8、R9和R10流域蔬菜和水稻的集约化生产是高养分浓度的主要来源。这种独特的变化,特别是在R5和R8流域,可能归因于其他不同的污染源。因此,对上述5个流域进行综合研究具有重要意义。这就是管理措施的效率,污染源的识别,以及河流中营养物质的增加与河流内生物群相互作用的程度。本研究提供了一种在相似气候条件下,利用有限的数据来评估流域中与人为干扰有关的WQ动态的方法。
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引用次数: 0
Factors affecting household’s willingness to pay for the fish conservation zones a case study from Khong District, Champasak Province, Laos 影响家庭为鱼类保护区付费意愿的因素——老挝占巴塞省孔县案例研究
Pub Date : 2022-09-05 DOI: 10.54279/mijeec.v4i1.247331
Salongxay Fongsamouth, Sakhone Keosoubinh, Philavanh Homsombath, Vilaphone Sounakeovongsa, Vannasinh Souvannasouk, Nitphone Senganatham, Vanphakonekham Koulavongsa
This research aims to estimate whether the WTP for fish conservation zones in terms of fish species protection areas. The authors used cross-sectional data that was collected from 369 respondents in 3 target villages in the Khong district of Champasak province Laos in 2021 and utilised WTP and maximum likelihood regression, marginal effect result for analysis. The estimate of the empirical model used for econometric analysis is based on the previously described model. This study finds that the average WTP households in target villages for fish conservation zones is 12.12 US $/household/year. The bid price, the recognition of the necessity of the fish conservation zones' knowledge, and average monthly income all positively impact WTP for fish conservation zones with a statistically significant impact. The authors conclude that the fish conservation zones might increase fish species in the study area. This valuation can be calculated as the cost to improve the quality of establishing fish conservation zones by multiplying the number of people in three villages of 768 households, which is the value expectation of the economy at 9,310 US $/year. However, improving public goods conservation now is the duty of everybody, so if we would like to strengthen fish conservation zones more efficiently than ever, we may find more suitable and sustainable alternatives.
本研究旨在从鱼类物种保护区的角度来评估WTP是否适合鱼类保护区。作者使用了2021年从老挝占巴塞省孔县3个目标村的369名受访者中收集的横断面数据,并利用WTP和最大似然回归、边际效应结果进行分析。用于计量经济分析的经验模型的估计是基于前面描述的模型。本研究发现,鱼类保护区目标村的平均家庭WTP为12.12美元/户/年。投标价格、对鱼类保护区知识必要性的认识、平均月收入对鱼类保护区WTP均有正向影响,且影响具有统计学意义。作者认为,鱼类保护区可能会增加研究区域的鱼类种类。这个估值可以计算为提高建立鱼类保护区质量的成本,乘以三个村庄768户的人口,即经济价值预期为9310美元/年。然而,现在改善公共物品保护是每个人的责任,所以如果我们想更有效地加强鱼类保护区,我们可能会找到更合适和可持续的替代方案。
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引用次数: 1
Beneficiation of organic black onion and lemon peel by-products through extraction and investigation of bioactive compounds 有机黑洋葱和柠檬皮副产物生物活性成分的提取和研究
Pub Date : 2022-08-28 DOI: 10.54279/mijeec.v4i2.248042
P. Bhuyar, Sarocha Jaichanta, Winitra Leelapattana, Suthira Sitthikun, N. Dussadee, R. Ramaraj, Y. Unpaprom
Much research on the shallot phytochemical is currently employed in various disciplines, such as medical reasons. Shallot (Allium ascalonicum) is a plant species belonging to the family Amaryllidaceae. It is a native plant grown in the North and Northeast region of Thailand. The present study aimed to determine the extracts' yield and antioxidant properties of black shallots made from fresh shallots. The black shallots were extracted with digestion by water, 47.5% and 95% ethanol at 50 ℃ for 1, 3 and 5 hours. The result discovered that black shallot extraction with digestion by water for 5 hours showed the highest weight of extracts (3.620 g). The determination of antioxidant activity by DPPH was observed, and extraction by water for 5 hours gave the highest percentage of inhibition. It was equal to 84.61%, which is not different from the digestion of 95% ethanol for 1 hour, with 83.48%. Similarly, the most outstanding value of IC50 was obtained from extraction with 95% ethanol for 1 and 5 hours with 21.30 mg/ml and 20.52 mg/ml. In terms of monitoring, the digestion method evaluated the productivity of pectin extracts from citrus peel. Hydrochloric acid (0.100, 0.050 and 0.025 M) was used as a solvent at 95 ℃ for 30, 60 and 90 minutes. The pectin extract by 0.1 M hydrochloric acid for 90 minutes gave the highest extract (5.237 g). The preliminary testing of cosmetic production was done by mixing black shallot extracts and pectin from citrus peels, and the two substances can be combined. Still, without adding preservatives, they could cause contamination in cosmetic samples.
目前对葱的植物化学成分进行了大量的研究,如医学上的原因。葱(Allium ascalonicum)是菊科植物。它是一种生长在泰国北部和东北部地区的本土植物。以新鲜青葱为原料,对黑青葱提取物的提取率和抗氧化性能进行了研究。用水、47.5%乙醇和95%乙醇在50℃条件下分别消化1、3、5小时。结果发现,水消化5小时的黑葱头提取液质量最高(3.620 g), DPPH法测定其抗氧化活性,水消化5小时的黑葱头提取液抑制率最高。为84.61%,与95%乙醇发酵1 h的83.48%无明显差异。同样,95%乙醇分别以21.30 mg/ml和20.52 mg/ml提取1小时和5小时的IC50值最为显著。在监测方面,消化法评价了柑橘果皮中果胶提取物的产率。以0.100、0.050、0.025 M盐酸为溶剂,在95℃条件下反应30、60、90分钟。果胶提取液经0.1 M盐酸处理90分钟,提取量最高(5.237 g)。将黑葱头提取液与柑橘果胶混合进行化妆品生产的初步试验,两者可以混合使用。不过,如果不添加防腐剂,它们可能会对化妆品样品造成污染。
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引用次数: 0
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Maejo International Journal of Energy and Environmental Communication
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