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Pretreatment of barley straw with acid and alkaline solutions to boost the efficiency of fermentable yield enzymatic degradation in the separated fermentation technique for ethanol production 在分离发酵技术中对大麦秸秆进行酸碱性预处理,提高可发酵产物酶降解效率
Pub Date : 2022-08-13 DOI: 10.54279/mijeec.v4i2.248224
Anodar Ratchawet, P. Chaiworn
Barley straw is a lignocellulose agricultural waste material that can be utilized as a raw material for ethanol production since it is easy to find, cheap, and has the potential to produce ethanol yield. This research aims to select the optimum pretreatment conditions to increase fermentable sugar production during enzymatic hydrolysis for bioethanol production. Initially, sulfuric acid at a concentration of 0.5, 1.0, 1.5, and 2.0% (v/v) pre-treated barley straw biomass was applied by autoclaving at 121℃ for 15 lb/in2 pressure for 15 min. Furthermore, acid-treated barley straw under the alkaline condition with calcium hydroxide concentrations of 1, 2, 3, and 4% (w/v) was autoclaved at 121℃ for 15 lb/in2 pressure for 15 min. Subsequently, the pretreatment of barley straw in the acetic and alkaline processes was compared. It was found that calcium hydroxide at a concentration of 2% (w/v) gave more sugar concentrations. Finally, combined acid and alkaline pretreatment with 2% of cellulase enzymatic hydrolysis had the highest total sugar concentration of 205.43 g/L and reducing sugar of 134.42 g/L, producing the highest ethanol yield (16.17 g/L) by 24 hours of fermentation.
大麦秸秆是一种木质纤维素农业废弃物,由于它容易找到,价格便宜,并且具有生产乙醇产量的潜力,因此可以用作乙醇生产的原料。本研究旨在选择最佳预处理条件,以提高酶解生产生物乙醇过程中可发酵糖的产量。首先,采用浓度分别为0.5、1.0、1.5和2.0% (v/v)的硫酸预处理大麦秸秆生物质,在121℃、15 lb/in2压力下蒸压15 min。然后,采用氢氧化钙浓度分别为1、2、3和4% (w/v)的碱性条件下,在121℃、15 lb/in2压力下蒸压15 min。随后,比较了醋酸和碱性工艺对大麦秸秆的预处理效果。结果表明,当氢氧化钙浓度为2% (w/v)时,糖浓度较高。最后,以2%纤维素酶水解的酸碱联合预处理,总糖浓度最高,为205.43 g/L,还原糖浓度最高,为134.42 g/L,发酵24小时乙醇产量最高,为16.17 g/L。
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引用次数: 0
The possibility of aquatic weeds serving as a source of feedstock for bioethanol production: a review 水生杂草作为生物乙醇生产原料的可能性综述
Pub Date : 2022-07-15 DOI: 10.54279/mijeec.v4i2.248180
Phuong Thi Vu, Rameshprabu Ramaraj, Prakash Bhuyar, Y. Unpaprom
Anaerobic digestion is recognized as an attractive option for the effective management and treatment of lignocellulosic biomass as well as waste recovery of resources for bioethanol production. Long enough testing has been done on bioethanol production using lignocellulosic biomass. This helps to reduce stress and global energy problems. Global wide has a variety of environmental impacts due to its use of fossil fuels. Bioethanol might be produced in Asian locations from many types of biomasses, including agricultural waste, forest waste, and wood biomass. This would be an environmentally friendly process. Unfortunately, there is very little research into the production of ethanol from rice field weeds. This makes it difficult to develop bioethanol production. This review is aimed at developing bioethanol production and the trend towards organic products that began nowadays. Unwanted weed growth is a major problem in rice cultivation. This review demonstrated the waste-to-energy aspect of the bioethanol production process using two weeds, gooseweed (Sphenoclea Zeylanica), and small-flowered nutsedge (Cyperus difformis).  
厌氧消化被认为是有效管理和处理木质纤维素生物质以及回收生物乙醇资源的一个有吸引力的选择。利用木质纤维素生物质生产生物乙醇的试验已经进行了很长时间。这有助于减少压力和全球能源问题。由于化石燃料的使用,全球范围内有各种各样的环境影响。生物乙醇可以在亚洲地区从多种类型的生物质中生产,包括农业废弃物、森林废弃物和木材生物质。这将是一个环境友好的过程。不幸的是,关于从稻田杂草中生产乙醇的研究很少。这使得发展生物乙醇生产变得困难。本文综述了生物乙醇生产的发展和有机产品的发展趋势。杂草生长是水稻种植的一个主要问题。这篇综述展示了利用两种杂草,鹅毛草(Sphenoclea Zeylanica)和小花核桃草(Cyperus diformis)生产生物乙醇的废物转化为能源方面。
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引用次数: 0
In-vitro antioxidant, the antimicrobial and anticarcinogenic activity of environment friendly nano formulation using Mangifera indica 芒果环保纳米制剂的体外抗氧化、抑菌和抗癌活性
Pub Date : 2022-07-10 DOI: 10.54279/mijeec.v4i2.248123
Velmurugan Kalamani, V. S, Shreya Ramesh, K. M. Moghan Prasad, Raja Selvaraju, M. Samynathan, Vandhana Gangadharan
The green synthesis of nanoparticles (NPs) from plant extracts is an innovative and considered as an effective method, which has many biological and therapeutic benefits. In this regard, silver nanoparticles were synthesized from leaves of Mangifera indica (Mango) and their properties were characterized. Morphological characterizations such as UV-Visible Spectroscopy (UV-Vis), Fourier-Transform Infra-red Spectroscopy (FTIR), and X-ray diffraction (XRD) confirm the physical characteristics of silver nano-particles. In addition, Mangifera indica phytochemicals possesses DPPH radical scavenging activity, antioxidant, and antibacterial properties. The nano formulation (phytochemicals + NPs) is also tested against the Hep G2 human cell line for anticarcinogenic properties. Nano formulation concentrations at 300μg/ml shown anticarcinogenic effect to Hep G2 human cell lines up to 53.29768%. The Bio pesticidal activity of the paddy pest Cicadella viridis (green leaf hopper) was checked through an antifeedant assay and the activity of the pest was decreased with the introduction of nano formulation. These results show better antioxidant, antibacterial and anticarcinogenic properties. Thus, they can be used to replace chemical therapeutic drugs.
以植物提取物为原料绿色合成纳米颗粒是一种创新的、被认为是有效的方法,具有许多生物学和治疗效益。为此,以芒果叶为原料合成了银纳米粒子,并对其性质进行了表征。紫外可见光谱(UV-Vis)、傅里叶变换红外光谱(FTIR)和x射线衍射(XRD)等形态学表征证实了银纳米颗粒的物理特性。此外,芒果植物化学物质还具有清除DPPH自由基、抗氧化和抗菌活性。纳米制剂(植物化学物质+ NPs)也对Hep G2人类细胞系进行了抗癌特性测试。纳米制剂浓度为300μg/ml时,对人Hep G2细胞株的抗癌作用高达53.29768%。通过对水稻害虫绿翅蝉(Cicadella viridis, green leaf hopper)的生物杀虫活性测定,纳米配方的引入降低了该害虫的生物杀虫活性。这些结果显示出较好的抗氧化、抗菌和抗癌性能。因此,它们可以用来取代化学治疗药物。
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引用次数: 0
Providing electricity access for unelectrified people in remote areas: demonstrated to a case study in Libya 为偏远地区未通电的人们提供电力:以利比亚的案例研究为例
Pub Date : 2022-06-24 DOI: 10.54279/mijeec.v4i2.247735
Noralden Mohamed Bauid, Md. Mizanur Rahman
Power generation in rural areas of the world, whether through renewable energy sources or grid expansion, is critical to increasing the monetary value of life. The cost of extending the national grid or installing off-grid systems is determined by a variety of factors, including the area's location, geography, population, distance from a grid point, and land size. Due to their cost-effectiveness and ease, off-grid rural electrification systems that incorporate a variety of renewable energy sources (RESs) have become unavoidable in areas where grid connectivity is neither available nor feasible. A hybrid combination of renewable energy technologies (RETs) has demonstrated to be a viable alternative to costly grid extension in remote areas throughout the world. The purpose of this study was to plan and assess the techno-economic feasibility of providing electricity to rural Enttelat in Libya using renewable energy sources, considering 70 houses with a combined load of 875 kWh/d. Three significant outcomes were obtained because of the techno-economic design using the HOMMER tool. These three primary outcomes were chosen for their resource availability and cost-effectiveness. When several input parameters such as annual average load, scaled annual average solar resource, wind speed, annual real interest rate, and solar PV and wind component prices were varied, the sensitivity analysis revealed that hybrid system solar PV-wind renewable resource has a high potential, especially if the location is remote from a grid source.
世界农村地区的发电,无论是通过可再生能源还是通过电网扩张,对于增加生命的货币价值至关重要。扩展国家电网或安装离网系统的成本由多种因素决定,包括该地区的位置、地理、人口、与电网点的距离和土地大小。由于其成本效益和易用性,包含各种可再生能源(RESs)的离网农村电气化系统在电网连接既不可用也不可行的地区已成为不可避免的。可再生能源技术的混合组合已被证明是世界各地偏远地区昂贵的电网扩展的可行替代方案。本研究的目的是规划和评估利用可再生能源向利比亚农村Enttelat供电的技术经济可行性,考虑到70户房屋的总负荷为875千瓦时/天。由于使用HOMMER工具进行技术经济设计,获得了三个显著的结果。选择这三个主要结局是根据它们的资源可用性和成本效益。当年平均负荷、按比例计算的年平均太阳能资源、风速、年实际利率以及太阳能光伏和风能组件价格等输入参数发生变化时,敏感性分析表明,混合系统的太阳能光伏-风能可再生资源具有很高的潜力,特别是在远离电网的情况下。
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引用次数: 0
Influence of nanoparticles inclusion on the production of bioethanol from corn stalks and leaves 纳米颗粒包合物对玉米秸秆和叶片生产生物乙醇的影响
Pub Date : 2022-06-09 DOI: 10.54279/mijeec.v4i2.248138
Nuttapong Saetang, Sawitree Tipnee
Bioethanol is a viable alternative to petroleum-derived fossil fuels. It is renewable, low-cost, and the preferred fuel for most developing countries worldwide. Although it is possible to make bioethanol from corn stalks and leaves wastes, second-generation biofuels made from agricultural waste feedstocks represent a significant step forward. In the present research, nickel oxide (NiO) nanoparticles (NPs) were used as a biocatalyst to achieve maximum ethanol output. The pretreatment of 2% NaOH with NiO NPs, 15-min autoclave condition, showed the highest total and reducing sugar yield was 162.69 g/L and 43.75 g/L. After hydrolysis, the suitable total and reducing sugar yield of 185.43 g/L and 125.42 g/L was chosen for further fermentation with the expansion of Saccharomyces cerevisiae cells. Separate hydrolysis and fermentation (SHF) using corn stalk and leaf waste was significantly assisted by incorporating a nanocatalyst; ethanol concentration was increased to 15.8 g/L at 24 hours incubation period. The study revealed critical information regarding ways NiO NPs could be employed to improve the efficiency of the ethanol production bioprocess.
生物乙醇是石油衍生化石燃料的可行替代品。它是可再生的、低成本的,是世界上大多数发展中国家的首选燃料。虽然从玉米秸秆和玉米叶废料中制造生物乙醇是可能的,但从农业废料原料中制造的第二代生物燃料是向前迈出的重要一步。在本研究中,使用氧化镍纳米颗粒(NiO)作为生物催化剂,以达到最大的乙醇产量。用NiO NPs预处理2% NaOH,蒸压条件为15 min,总糖和还原糖产量最高,分别为162.69 g/L和43.75 g/L。水解后,选择适宜的总糖和还原糖产量分别为185.43 g/L和125.42 g/L,扩大酿酒酵母细胞进行进一步发酵。纳米催化剂对玉米秸秆和叶片废弃物的分离水解和发酵(SHF)有显著的促进作用;孵育24小时后,乙醇浓度提高到15.8 g/L。该研究揭示了NiO NPs可用于提高乙醇生产生物过程效率的关键信息。
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引用次数: 0
Gentiana kurro’s physiology and pharmacological properties: Recent update and future perspective 龙胆的生理和药理特性:最新进展和未来展望
Pub Date : 2022-06-04 DOI: 10.54279/mijeec.v4i2.247692
Aditi Sharma, M. D. Sharma, P. Bhuyar, P. Mishra, S. Kulshreshtha
Gentiana kurroo royle is an endangered bitter medicinal plant of the Indian subcontinent. This medicinal plant mainly grows in Kashmir, Himachal Pradesh, and North-west Himalayas. The medicinal plant's root and rhizome are frequently used by native people for various local remedies. This species has become critically endangered due to its endemic nature and high rate of extraction from its natural environment. In India rhizome and root of this medicinal plant are used as ant periodic, bitter tonics, anti-inflammatory, blood purifier, expectorant, antipsychotic, carminative, and stomachic. Genitiana kurroo can be propagated through micro-filtration of seeds, rhizome cutting, shoot-made segments, and somatic embryogenesis. The roots of Genitiana kurroo are rich in various ingredients such as flavonoids, alkaloids, and terpenoids which are responsible for analgesic, anticancer, and immune-modular effects. Considering these perspectives the present review has intended to provide updated prospects of Gentiana kurroo and provides insights on the nutritional and various pharmacological properties based on updated research literature. This review explores the use of  Gentiana kurroo across disciplines for its wide range of prominent-pharmacological properties.
龙胆是印度次大陆的一种濒临灭绝的苦药用植物。这种药用植物主要生长在克什米尔、喜马偕尔邦和西北喜马拉雅地区。这种药用植物的根和根茎经常被当地人用于各种地方疗法。由于其特有的性质和从自然环境中提取的高速率,该物种已成为极度濒危物种。在印度,这种药用植物的根茎和根被用作蚂蚁周期,苦味滋补,消炎,血液净化,祛痰,抗精神病,驱风,和胃。龙胆可通过种子微滤、根茎切割、芽成节和体胚发生进行繁殖。龙胆根富含黄酮类、生物碱、萜类等多种成分,具有镇痛、抗癌、免疫等作用。考虑到这些方面,本综述旨在提供龙胆的最新前景,并在最新研究文献的基础上对龙胆的营养和各种药理特性提供见解。这篇综述探讨了龙胆草跨学科的使用,因为它具有广泛的突出的药理特性。
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引用次数: 0
Optimization and sensitivity analysis of high frequency pulse tube cryocooler for aerospace application 航天用高频脉冲管制冷机的优化与灵敏度分析
Pub Date : 2022-05-17 DOI: 10.54279/mijeec.v4i2.247992
Damu Chakala, Sumukh Moudghalya, Mrunal Nerale, Rajendra Prasad, U. Behera, Sathyanarayana Reddy
The demand for cryogenic cooling systems has been on the rise ever since the development of long-range infrared imaging systems, especially high-frequency stirling-type cryocoolers which have a vast array of applications in the aerospace sector. While there have been many studies reporting analysis of the pulse tube cryocoolers, these lack an optimization approach with a further sensitivity analysis from computational fluid dynamic (CFD) data to obtain better cold end temperatures. Presently, a response surface method (RSM), has been utilized to characterize the influence of the affecting parameters and a global sensitivity analysis has been conducted to put forward the critical parameters that influence the performance of the device. It is observed that an increase in frequency for lower pressure ratios with higher mean pressure results in better cooling performance in smaller pulse tube diameter models. The sensitivity analysis indicates the operating parameters such as frequency and pressure ratios to be more influential than the geometric parameters with an impact of over 80%.
自远程红外成像系统发展以来,对低温冷却系统的需求一直在上升,特别是在航空航天领域有着广泛应用的高频斯特林型制冷机。虽然有许多研究报告了对脉管制冷机的分析,但这些研究都缺乏一种优化方法,可以通过计算流体动力学(CFD)数据进行进一步的灵敏度分析,以获得更好的冷端温度。目前,利用响应面法(RSM)表征影响参数的影响,并进行全局灵敏度分析,提出影响器件性能的关键参数。在较小的脉冲管径模型中,在较低的压力比和较高的平均压力下,增加频率可以获得较好的冷却性能。灵敏度分析表明,频率比、压力比等操作参数比几何参数的影响更大,影响幅度在80%以上。
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引用次数: 0
Anaerobic digestion of food waste from fruits and vegetables to improve stability and effectiveness 厌氧消化来自水果和蔬菜的食物垃圾,以提高稳定性和有效性
Pub Date : 2022-04-30 DOI: 10.54279/mijeec.v4i1.248063
Nuttapong Saetang, Sawitree Tipnee
Anaerobic digestion is a process that may be used to handle food scraps, and it is a method that is both effective and efficient. The biogas that is generated as a byproduct of this process has the potential to be utilized as a renewable source of bioenergy. Anaerobic digestion is a promising option to manage and treat food wastes and recover resources. To determine the efficiency of methane production, batch biochemical methane pot testing was performed. This paper reviews the system stability and efficiency of additives in anaerobic digestion. It is possible to produce biogas at different temperatures (27±2°C, 35±2°C, and 45±2°C). Biogas enhancement is dependent on temperature. The experiments were conducted for a 45-day digestion period.  The 35±2°C fermenter produced the highest total biogas yield of 13093.55 ml and the highest methane content of 63.71%. The study was designed to determine the optimal temperature for increasing household levels in future applications.
厌氧消化是一种可以用来处理食物残渣的过程,它是一种既有效又高效的方法。作为这一过程的副产品产生的沼气有潜力被用作生物能源的可再生来源。厌氧消化是管理和处理食物垃圾和回收资源的一个很有前途的选择。为确定产甲烷效率,进行了间歇式生化甲烷罐试验。本文综述了添加剂在厌氧消化中的系统稳定性和效率。可以在不同温度(27±2°C, 35±2°C和45±2°C)下生产沼气。沼气强化取决于温度。试验进行45 d消化期。35±2℃发酵罐总沼气产量最高,为13093.55 ml,甲烷含量最高,为63.71%。该研究旨在确定未来应用中增加家庭水平的最佳温度。
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引用次数: 1
Image analysis reveals cellular fragmentation of Spirulina platensis upon treatment with heavy metal ions 图像分析显示重金属离子处理后的螺旋藻细胞碎裂
Pub Date : 2022-04-24 DOI: 10.54279/mijeec.v4i1.247678
Lekha Kaushik, Dinakari Sarangan, Deenadayalan Karaiyagowder Govindarajan, Sowmiya Muthumanickam, M. Sivaramakrishnan, Ram Kothandan, Suganthy Masanamani, Saraswathy Nachimuthu, H. Mamane, Kumaravel Kandaswamy
Bioremediation is a promising technique that can be used to decrease the environmental discharge of Heavy Metal (HM) such as copper, and zinc. Although studies have addressed the cytotoxic effects of HMs, however, the effect of HMs on the geometry of bacterial species such as Spirulina platensis (S. platensis) remains unknown. Quantitative analysis of parameters such as cell perimeter, cell count, and cell distribution shall greatly improve the efficiency of environmental monitoring. Therefore, this study demonstrates the use of an open-source image analysis tool (ImageJ/Fiji) to quantify the aforementioned parameters to analyze the extent of damage caused by lethal concentration of > 2 mg/L of Cu2+ and > 6 mg/L of Zn2+ which disintegrates S. platensis cells into smaller fragments and subsequently affects their structural parameters in perimeter and cell distribution. In summary, this article demonstrates the use of an image analysis platform to quantify the geometric parameters of microbes for environmental monitoring.
生物修复是一种很有前途的技术,可用于减少铜、锌等重金属的环境排放。虽然研究已经解决了HMs的细胞毒性作用,然而,HMs对螺旋藻(S. platensis)等细菌物种的几何结构的影响仍然未知。对细胞周长、细胞数量和细胞分布等参数的定量分析将大大提高环境监测的效率。因此,本研究将利用开源图像分析工具(ImageJ/Fiji)对上述参数进行量化,分析> 2 mg/L Cu2+和> 6 mg/L Zn2+的致死浓度对S. platensis细胞的损伤程度,这些Cu2+和> 6 mg/L Zn2+将S. platensis细胞分解成更小的碎片,从而影响其周长和细胞分布的结构参数。综上所述,本文演示了使用图像分析平台来量化微生物的几何参数以进行环境监测。
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引用次数: 2
Effect of agitation on extraction of phenolic compounds from ripe pericarp of Wodyetia bifurcata 搅拌对双叶木参成熟果皮中酚类化合物提取的影响
Pub Date : 2022-04-10 DOI: 10.54279/mijeec.v4i1.246121
Thanapat Sakdasukon, S. Thanachasai
In this study, the ripe pericarp extracts of Wodyetia bifurcata were prepared by solvent extraction techniques with and without agitation. The influence of agitation was examined on the extraction yield, total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of the ripe pericarp extracts of Wodyetia bifurcata. Antioxidant capacity of extracts was evaluated by measuring the scavenging effect on 2, 2’-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) radicals. Compared to solvent extraction without agitation, solvent extraction with agitation significantly (p < 0.05) increased the extraction yield, TPC, TFC and antioxidant capacities (ABTS and DPPH) of the ripe pericarp extracts of Wodyetia bifurcata. In addition, higher agitation speed resulted in higher extraction yield, TPC, TFC and antioxidant activities (ABTS and DPPH) of the ripe pericarp extracts of Wodyetia bifurcata.
本研究采用溶剂萃取法和无搅拌萃取法分别制备了双曲木耳成熟果皮提取物。研究了搅拌条件对双叶树成熟果皮提取物提取率、总酚含量(TPC)、总黄酮含量(TFC)和抗氧化活性的影响。通过测定提取物对2,2′-氮唑(3-乙基苯并噻唑-6-磺酸)(ABTS)和2,2′-二苯基-1-吡啶肼(DPPH)自由基的清除作用,评价其抗氧化能力。与不搅拌的溶剂提取相比,有搅拌的溶剂提取显著(p < 0.05)提高了双歧木叶成熟果皮提取物的提取率、TPC、TFC和抗氧化能力(ABTS和DPPH)。此外,搅拌速度越快,双叶树成熟果皮提取物的提取率、TPC、TFC和抗氧化活性(ABTS和DPPH)越高。
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引用次数: 0
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Maejo International Journal of Energy and Environmental Communication
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