M. Stella, Radheshyam Sharma, S. Nema, R. Ramakrishnan, Ashish Kumar
Background: Plant growth promoting rhizobia play an important in agricultural ecosystem through symbiotic association with a wide range of leguminous plants. Legume-rhizobia association is a host specific symbiosis hence the need to identify the strains and the diversity of rhizobia associated with specific type of legume for better bio-prospecting of the associated benefits. A wide range of variation is present among the distribution of rhizobia and greatly affected by geographical locality, edaphic factors and environmental variation with time and space. Molecular techniques have been developed to aid the traditional phenotypic and morpho-cultural techniques in distinguishing the different microbial genera, species and strains. The present study aimed at isolation and morpho-molecular characterization of rhizobia from root nodule of green gram cultivated in central part of India.Methods: In the field-laboratory investigation during 2018-2019, various locations of central India with contrasting agro climatic conditions were surveyed and rhizobia trapping done. A total of 40 rhizobia were retrieved from nodules of green gram and characterized in laboratory based on morphological, biochemical and molecular techniques and results validated for taxonomic identification.Result: In our investigation all forty isolated rhizobia were found phosphate solubilizers, 38 IAA producers and 37 ammonia excretors and grew well at 28oC and 37oC. In carbohydrate fermentation test 34 isolates changed the broth colour from red to yellow with gas formation in durum tubes. Twenty diverse rhizobia isolates were selected with respect to their multifunctional properties and studied for molecular characterization. Based on 16S rRNA gene sequencing, these bacterial strains were identified under two genera: Rhizobium and Brady Rhizobium. Phylogenetic analysis divided 20 rhizobia isolates into two clusters. Major group included 12 strains and minor group included 8 strains.
{"title":"Genetic Characterization and Diversity of Rhizobia Isolated from Root Nodules of Green Gram (Vigna radiata L.) found in Central Plateau of India","authors":"M. Stella, Radheshyam Sharma, S. Nema, R. Ramakrishnan, Ashish Kumar","doi":"10.18805/LR-4258","DOIUrl":"https://doi.org/10.18805/LR-4258","url":null,"abstract":"Background: Plant growth promoting rhizobia play an important in agricultural ecosystem through symbiotic association with a wide range of leguminous plants. Legume-rhizobia association is a host specific symbiosis hence the need to identify the strains and the diversity of rhizobia associated with specific type of legume for better bio-prospecting of the associated benefits. A wide range of variation is present among the distribution of rhizobia and greatly affected by geographical locality, edaphic factors and environmental variation with time and space. Molecular techniques have been developed to aid the traditional phenotypic and morpho-cultural techniques in distinguishing the different microbial genera, species and strains. The present study aimed at isolation and morpho-molecular characterization of rhizobia from root nodule of green gram cultivated in central part of India.Methods: In the field-laboratory investigation during 2018-2019, various locations of central India with contrasting agro climatic conditions were surveyed and rhizobia trapping done. A total of 40 rhizobia were retrieved from nodules of green gram and characterized in laboratory based on morphological, biochemical and molecular techniques and results validated for taxonomic identification.Result: In our investigation all forty isolated rhizobia were found phosphate solubilizers, 38 IAA producers and 37 ammonia excretors and grew well at 28oC and 37oC. In carbohydrate fermentation test 34 isolates changed the broth colour from red to yellow with gas formation in durum tubes. Twenty diverse rhizobia isolates were selected with respect to their multifunctional properties and studied for molecular characterization. Based on 16S rRNA gene sequencing, these bacterial strains were identified under two genera: Rhizobium and Brady Rhizobium. Phylogenetic analysis divided 20 rhizobia isolates into two clusters. Major group included 12 strains and minor group included 8 strains.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"36 1","pages":"353-361"},"PeriodicalIF":0.8,"publicationDate":"2020-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82299894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamed Milad Draweel, A. Soegianto, L. Soetopo, K. Kuswanto
Background: Bambara groundnut has the ability to drought tolerance and has high nutritional value and is one than Legume crops untapped, which need attention. Therefore in this research, it will look at the relationship between osmotic stress caused by a concentration of PEG and drought tolerance to get some genotypes has ability drought tolerance.Methods: The experiment was conducted in the Tissue culture Laboratory, Department of Agronomy, Faculty of Agriculture, the University of Brawijaya, Malang, Indonesia in January-March 2019. The objective of the experiment was an evaluation of some morphological criteria to drought tolerance on the seedling for thirty genotypes of Bambara groundnut using polyethylene glycol (PEG) 6000 by three concentrations (0%, 5% and 10%). A complete randomized design was used for this experiment with three replicates.Result: The 10% PEG was the most suitable concentration to select Bambara groundnut lines for drought tolerance. Genotypes give different responses to concentrations of PEG. In susceptible lines, there are germination constraints, such as Prevent and germination delayed, the formation of hypocotyl length, root length, fresh weight and dry matter, while drought-tolerant lines can germinate normally. Obtained six lines that were tolerant to drought, five local, namely BBL 1.1, PWBG 5.2.1, PWBG 6, SS 2.4.2, SS 3.4.2 and one from Thailand which is Tvsu 86.
{"title":"Evaluation of Some Morphological Criteria to Drought Tolerance on Seedling of Bambara Groundnut [Vigna subterranea (L.) Verdc.] using Polyethylene Glycol (PEG6000)","authors":"Mohamed Milad Draweel, A. Soegianto, L. Soetopo, K. Kuswanto","doi":"10.18805/LR-554","DOIUrl":"https://doi.org/10.18805/LR-554","url":null,"abstract":"Background: Bambara groundnut has the ability to drought tolerance and has high nutritional value and is one than Legume crops untapped, which need attention. Therefore in this research, it will look at the relationship between osmotic stress caused by a concentration of PEG and drought tolerance to get some genotypes has ability drought tolerance.Methods: The experiment was conducted in the Tissue culture Laboratory, Department of Agronomy, Faculty of Agriculture, the University of Brawijaya, Malang, Indonesia in January-March 2019. The objective of the experiment was an evaluation of some morphological criteria to drought tolerance on the seedling for thirty genotypes of Bambara groundnut using polyethylene glycol (PEG) 6000 by three concentrations (0%, 5% and 10%). A complete randomized design was used for this experiment with three replicates.Result: The 10% PEG was the most suitable concentration to select Bambara groundnut lines for drought tolerance. Genotypes give different responses to concentrations of PEG. In susceptible lines, there are germination constraints, such as Prevent and germination delayed, the formation of hypocotyl length, root length, fresh weight and dry matter, while drought-tolerant lines can germinate normally. Obtained six lines that were tolerant to drought, five local, namely BBL 1.1, PWBG 5.2.1, PWBG 6, SS 2.4.2, SS 3.4.2 and one from Thailand which is Tvsu 86.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"488 1","pages":"175-184"},"PeriodicalIF":0.8,"publicationDate":"2020-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72457578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Lentil is an oldest domesticated grain legume in the world. It is cultivated during winter season in India which is a major source of protein (28%) for human consumption. The present study was undertaken to study association analysis of grain yield with other quantitative traits and to estimate direct and indirect effects of various traits on grain yield.Methods: The experiment was carried out using 53 advance breeding lines of lentil in a Randomized Complete Block Design and replicated thrice.Result: The investigation revealed that days to 50% flowering, days to 75% maturity, plant height, pods/plant, biological yield/plant and grain yield/plant have significant differences and wide range of variations during both the year.Pods/plant exhibited high genotypic coefficient of variation, heritability and genetic advance signifying the effects of additive gene effect. Pods/plant and biological yield/plant showed positive and significant correlations with grain yield /plant and also have positive direct effects on grain yield. Therefore, this investigation suggested that pods/plant and biological yield/plant may be focused during selection for genetic gain in lentil.
{"title":"Genetic Variability and Association Analysis for Various Agro Morphological Traits in Lentil (Lens culinaris M.)","authors":"Ashok Kumar, R. K. Gill, Mandeep Singh","doi":"10.18805/LR-4326","DOIUrl":"https://doi.org/10.18805/LR-4326","url":null,"abstract":"Background: Lentil is an oldest domesticated grain legume in the world. It is cultivated during winter season in India which is a major source of protein (28%) for human consumption. The present study was undertaken to study association analysis of grain yield with other quantitative traits and to estimate direct and indirect effects of various traits on grain yield.Methods: The experiment was carried out using 53 advance breeding lines of lentil in a Randomized Complete Block Design and replicated thrice.Result: The investigation revealed that days to 50% flowering, days to 75% maturity, plant height, pods/plant, biological yield/plant and grain yield/plant have significant differences and wide range of variations during both the year.Pods/plant exhibited high genotypic coefficient of variation, heritability and genetic advance signifying the effects of additive gene effect. Pods/plant and biological yield/plant showed positive and significant correlations with grain yield /plant and also have positive direct effects on grain yield. Therefore, this investigation suggested that pods/plant and biological yield/plant may be focused during selection for genetic gain in lentil.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"206 1","pages":"776-779"},"PeriodicalIF":0.8,"publicationDate":"2020-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80424880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amit Kumar, A. Dhaka, Satish Kumar, Samunder Singh, Ishmadhu
Background: Among pulse crops, pigeon pea [Cajanus cajan (L.) Millsp] is one of the most important pulse crops of India after chick pea. Among various biotic factors limiting pigeon pea yield, weeds are of prime importance which can causes seed yield losses up to 80 per cent. Therefore, weed management is an important key factor for enhancing the productivity of pigeon pea. Imazethapyr, an herbicide of imidazolinones group has been registered for soybean, groundnut and other legumes. Various trials in different regions were conducted to study the effects of imazethapyr alone or in combination for controlling the weeds in different crops but in pigeon pea limited research were conducted. Therefore, current study aimed to study the effect of imidazolinones alone and in combination on weed dry matter accumulation in pigeon pea (Cajanus cajan L.).Methods: Pre and post emergence application of imidazolinones alone and in combination was done and dry matter accumulation by different types of weeds i.e. grassy, broad leaf and sedges in pigeon pea crop were worked out at different interval of crop span.Result: Among all weed controls treatments, two hoeing at 40 and 70 DAS found most effective to control all kind of weeds. Alone application of pendimethalin was not effective to control sedges. PRE application of imidazolinones was comparatively more effective than POE application. Application of imidazolinones in combination with pendimethalin improves its efficacy to control total weeds than alone application of it. Therefore, PRE application of pendimethalin + imazethapyr @ 1000 + 75 g ha-1 proved to be an effective and a profitable alternative to the existing recommendation (two hoeing at 25 and 45 DAS) of weed control in pigeon pea in Haryana locality.
{"title":"Effect of Imidazolinones Alone and in Combination on Weed Dry Matter Accumulation in Pigeon Pea (Cajanus cajan L.)","authors":"Amit Kumar, A. Dhaka, Satish Kumar, Samunder Singh, Ishmadhu","doi":"10.18805/LR-4250","DOIUrl":"https://doi.org/10.18805/LR-4250","url":null,"abstract":"Background: Among pulse crops, pigeon pea [Cajanus cajan (L.) Millsp] is one of the most important pulse crops of India after chick pea. Among various biotic factors limiting pigeon pea yield, weeds are of prime importance which can causes seed yield losses up to 80 per cent. Therefore, weed management is an important key factor for enhancing the productivity of pigeon pea. Imazethapyr, an herbicide of imidazolinones group has been registered for soybean, groundnut and other legumes. Various trials in different regions were conducted to study the effects of imazethapyr alone or in combination for controlling the weeds in different crops but in pigeon pea limited research were conducted. Therefore, current study aimed to study the effect of imidazolinones alone and in combination on weed dry matter accumulation in pigeon pea (Cajanus cajan L.).Methods: Pre and post emergence application of imidazolinones alone and in combination was done and dry matter accumulation by different types of weeds i.e. grassy, broad leaf and sedges in pigeon pea crop were worked out at different interval of crop span.Result: Among all weed controls treatments, two hoeing at 40 and 70 DAS found most effective to control all kind of weeds. Alone application of pendimethalin was not effective to control sedges. PRE application of imidazolinones was comparatively more effective than POE application. Application of imidazolinones in combination with pendimethalin improves its efficacy to control total weeds than alone application of it. Therefore, PRE application of pendimethalin + imazethapyr @ 1000 + 75 g ha-1 proved to be an effective and a profitable alternative to the existing recommendation (two hoeing at 25 and 45 DAS) of weed control in pigeon pea in Haryana locality.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"52 1","pages":"826-831"},"PeriodicalIF":0.8,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77217009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: In this decade, increasing temperatures and adverse environmental factors are expected to influence the crop yield, seed quality and germination ability. Prolonged heat stress affects the pod setting, seed morphology and alters the starch granules.Methods: In this study, Scanning Electron Microscopy (SEM) and Light microscope was used to investigate the variations of morphological and ultra-structural characteristics in blackgram genotypes grown under high temperature (38+2°C) stress (HTS). Nineteen blackgram genotypes were evaluated under open top temperature controlled chamber (OTC) and the genotypes were classified into three categories such as heat tolerant, moderately heat tolerant and heat susceptible genotypes based on the Temperature Induction Response (TIR) and yield reduction percentage under HTS. Two genotypes were selected and examined the seed size, surface pattern, starch granule size and hilum from the each category.Result: Seed coat morphology was captured using SEM and light microscope showed that the tolerant genotype VBG-07-001 had a clear shiny surface, thin and reduced cotyledon fissures, compact surface devoid of surface deposits and pits whereas rest of the genotypes (VBG-06-001, VBN-6, CoBg-11-02, CoBg-11-03 and VBG-08-003) had a rough surface. A bold and well-structured starch granule was observed in heat tolerant lines whereas, unstructured and pleated granules were observed in moderately tolerant and heat stress lines. These morphological and ultra-structural studies revealed remarked diversity among different genotypes that can be regarded as useful to screen the genotypic tolerant characters for HTS.
{"title":"Morphological and Ultra-Structural Characterization of Blackgram [Vigna mungo (L.) Hepper] Genotypes Grown under High Temperature Stress","authors":"C. Partheeban, H. Vijayaraghavan","doi":"10.18805/LR-4273","DOIUrl":"https://doi.org/10.18805/LR-4273","url":null,"abstract":"Background: In this decade, increasing temperatures and adverse environmental factors are expected to influence the crop yield, seed quality and germination ability. Prolonged heat stress affects the pod setting, seed morphology and alters the starch granules.Methods: In this study, Scanning Electron Microscopy (SEM) and Light microscope was used to investigate the variations of morphological and ultra-structural characteristics in blackgram genotypes grown under high temperature (38+2°C) stress (HTS). Nineteen blackgram genotypes were evaluated under open top temperature controlled chamber (OTC) and the genotypes were classified into three categories such as heat tolerant, moderately heat tolerant and heat susceptible genotypes based on the Temperature Induction Response (TIR) and yield reduction percentage under HTS. Two genotypes were selected and examined the seed size, surface pattern, starch granule size and hilum from the each category.Result: Seed coat morphology was captured using SEM and light microscope showed that the tolerant genotype VBG-07-001 had a clear shiny surface, thin and reduced cotyledon fissures, compact surface devoid of surface deposits and pits whereas rest of the genotypes (VBG-06-001, VBN-6, CoBg-11-02, CoBg-11-03 and VBG-08-003) had a rough surface. A bold and well-structured starch granule was observed in heat tolerant lines whereas, unstructured and pleated granules were observed in moderately tolerant and heat stress lines. These morphological and ultra-structural studies revealed remarked diversity among different genotypes that can be regarded as useful to screen the genotypic tolerant characters for HTS.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"21 1","pages":"757-763"},"PeriodicalIF":0.8,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87483425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yongli Zhang, Lang Qian, Zhennan Wang, Qingping Zhang, Hui Song
Background: Plants in the genus Arachis produce flowers aerially that develop into gynophores that grow into the ground, where they develop into fruits. Although some aerial gynophores develop into pods aboveground, embryo or seed abortion occurs in these individuals. During pod development, the shell wall initially comprises the majority of the fruit volume, but then the seeds expand up to the total pod volume. Expansins are plant cell wall-loosening proteins involved in cell enlargement and a variety of other developmental processes in which cell-wall modification occurs.Methods: In this study, we analyzed the expansin genes during seed development using RNA-seq data by bioinformatic approaches.Results: We identified five expansin genes exhibited up-regulated expression, while four genes were down-regulated expression using Arachis duranensis RAN-seq data. Multiple transcription factors regulate AdEXPs throughout seed development. Genes co-expressed with AdEXPs are involved in metabolic processes throughout pod development into seed development, reframing the current understanding of the novel subterranean peanut fruits.
{"title":"The Role of the Expansin Gene in the Process of Novel Subterranean Seed Development in Arachis duranensis","authors":"Yongli Zhang, Lang Qian, Zhennan Wang, Qingping Zhang, Hui Song","doi":"10.18805/LR-525","DOIUrl":"https://doi.org/10.18805/LR-525","url":null,"abstract":"Background: Plants in the genus Arachis produce flowers aerially that develop into gynophores that grow into the ground, where they develop into fruits. Although some aerial gynophores develop into pods aboveground, embryo or seed abortion occurs in these individuals. During pod development, the shell wall initially comprises the majority of the fruit volume, but then the seeds expand up to the total pod volume. Expansins are plant cell wall-loosening proteins involved in cell enlargement and a variety of other developmental processes in which cell-wall modification occurs.Methods: In this study, we analyzed the expansin genes during seed development using RNA-seq data by bioinformatic approaches.Results: We identified five expansin genes exhibited up-regulated expression, while four genes were down-regulated expression using Arachis duranensis RAN-seq data. Multiple transcription factors regulate AdEXPs throughout seed development. Genes co-expressed with AdEXPs are involved in metabolic processes throughout pod development into seed development, reframing the current understanding of the novel subterranean peanut fruits.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"277 1","pages":"805-811"},"PeriodicalIF":0.8,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72528061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
X. Ke, L. Yin, J. Xu, W. Sun, X. Xu, Y. Guo, Y. Zuo
The objective of the study was to identify suitable reference genes that can be used for quantitative real-time PCR (qPCR) analysis in mung bean (Vigna radiata). Therefore, 10 potential reference genes were selected and the results showed that ubiquitin-conjugating enzyme was suitable as reference under drought and pathogen infection stress; elongation factor 1-a was the most stable gene under waterlogging; and actin performed the best under saline stress. These selected reference genes were further confirmed by analysis of the expression profiles of catalase and peroxidase under waterlogging. Our results will contribute to the improvement of the accuracy of gene expression evaluation in mung bean.
{"title":"Reference Genes for Quantitative Real-Time PCR Analysis of Gene Expression in Mung Bean under Abiotic Stress and Cercospora canescens Infection","authors":"X. Ke, L. Yin, J. Xu, W. Sun, X. Xu, Y. Guo, Y. Zuo","doi":"10.18805/lr-507","DOIUrl":"https://doi.org/10.18805/lr-507","url":null,"abstract":"The objective of the study was to identify suitable reference genes that can be used for quantitative real-time PCR (qPCR) analysis in mung bean (Vigna radiata). Therefore, 10 potential reference genes were selected and the results showed that ubiquitin-conjugating enzyme was suitable as reference under drought and pathogen infection stress; elongation factor 1-a was the most stable gene under waterlogging; and actin performed the best under saline stress. These selected reference genes were further confirmed by analysis of the expression profiles of catalase and peroxidase under waterlogging. Our results will contribute to the improvement of the accuracy of gene expression evaluation in mung bean.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"32 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2020-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74513192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Revathi, M. Arun, K. Sukumar, S. Dhrua, G. S. M. Bindu
A field experiment was conducted during kharif 2014 2015 in the Agricultural Research Station, Adilabad, Telangana to assess the effect of straw mulch and anti – transpirants on growth and yield of soybean. The treatment consisted of A) Antitranspirants (5) (spray at 15 days after flower initiation): T1 MgCO3 @ 5% T2 Glycerol @ 5% T3 KNO3 @ 1% T4 NaCO3 @ 5% T5 Control (water spray) B). Straw mulch @ 5 t/ ha after sowing C) Control (no mulch). Among the different anti transpirants, Na2CO3 @ 5% recorded higher mean seed yield (2862 kg/ ha) which was at par with Glycerol @ 5% (2762 kg/ ha) and MgCO3 @ 5% (2731 kg/ ha). Mulch (straw mulch @ 5 t/ ha) was significantly superior over control (without mulch) with highest mean seed yield (2723 kg/ ha). The combination of straw mulch and anti – transpirant can be recommended as an effective with respect to yield and cost for the soybean under rainfed condition.
{"title":"Enhancing the Water Stress Tolerance in Soybean (Glycine max L.) through Anti-Transpirants and Mulch","authors":"P. Revathi, M. Arun, K. Sukumar, S. Dhrua, G. S. M. Bindu","doi":"10.18805/lr-4216","DOIUrl":"https://doi.org/10.18805/lr-4216","url":null,"abstract":"A field experiment was conducted during kharif 2014 2015 in the Agricultural Research Station, Adilabad, Telangana to assess the effect of straw mulch and anti – transpirants on growth and yield of soybean. The treatment consisted of A) Antitranspirants (5) (spray at 15 days after flower initiation): T1 MgCO3 @ 5% T2 Glycerol @ 5% T3 KNO3 @ 1% T4 NaCO3 @ 5% T5 Control (water spray) B). Straw mulch @ 5 t/ ha after sowing C) Control (no mulch). Among the different anti transpirants, Na2CO3 @ 5% recorded higher mean seed yield (2862 kg/ ha) which was at par with Glycerol @ 5% (2762 kg/ ha) and MgCO3 @ 5% (2731 kg/ ha). Mulch (straw mulch @ 5 t/ ha) was significantly superior over control (without mulch) with highest mean seed yield (2723 kg/ ha). The combination of straw mulch and anti – transpirant can be recommended as an effective with respect to yield and cost for the soybean under rainfed condition.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"144 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2020-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77535907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cowpea (Vigna unguiculata (L.) Walp is used as a vegetable and feedcrop worldwide, but knowledge is limited regarding the variability and correlations for protein and seed traits within a cowpea core collection. We analyzed protein content and seed trait data from the cowpea core collection representing 111 accessions. The accession PI 354729 produced the significantly highest protein content of 25.527%. Eight Indian cowpea accessions produced significantly high protein content averaging 24.423%. Protein content was significantly correlated with seed pattern (r2 = 0.274***), seed texture (r2 = 0.346***), seed coat color (r2 = 0.136*) and seed pattern significantly correlated with seed texture (r2 = 0.197**). However, protein content showed a significant negative correlation (r2 =-0.333***) with 100 seed weight. One hundred seed weight (gm) showed significantly negative correlations with seed pattern (r2 = -0.191**) and seed texture (r2 = -0.265***). Seed coat color showed a significant negative correlation with seed pattern (r2 = -0.14). Based on these results, potential cowpea cultivars could be developed with improved protein content and morphological seed traits.
{"title":"Protein Content and Seed Trait Analysis in a Subset of the USDA, ARS, PGRCU Cowpea [Vigna unguiculata (L.) Walp.] Core Collection","authors":"J. Morris, B. Tonnis, M. Wang","doi":"10.18805/LR-529","DOIUrl":"https://doi.org/10.18805/LR-529","url":null,"abstract":"Cowpea (Vigna unguiculata (L.) Walp is used as a vegetable and feedcrop worldwide, but knowledge is limited regarding the variability and correlations for protein and seed traits within a cowpea core collection. We analyzed protein content and seed trait data from the cowpea core collection representing 111 accessions. The accession PI 354729 produced the significantly highest protein content of 25.527%. Eight Indian cowpea accessions produced significantly high protein content averaging 24.423%. Protein content was significantly correlated with seed pattern (r2 = 0.274***), seed texture (r2 = 0.346***), seed coat color (r2 = 0.136*) and seed pattern significantly correlated with seed texture (r2 = 0.197**). However, protein content showed a significant negative correlation (r2 =-0.333***) with 100 seed weight. One hundred seed weight (gm) showed significantly negative correlations with seed pattern (r2 = -0.191**) and seed texture (r2 = -0.265***). Seed coat color showed a significant negative correlation with seed pattern (r2 = -0.14). Based on these results, potential cowpea cultivars could be developed with improved protein content and morphological seed traits.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"249 1","pages":"495-500"},"PeriodicalIF":0.8,"publicationDate":"2020-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83725810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The flowering process at single plant level in soybean is a continuous dynamic system. Whether a flower can survive to mature depends on where it is located and when it is initiated. A field experiment was conducted to analyse the effect of flowering asynchrony on single flower survival and response of flowers or pods distribution to light enrichment. Our data suggest that early flowers (number 1-5) definitely survive and develop into mature pod because they don’t encounter fierce assimilates competition from other flowers or pods. Later flowers are usually prone to abortion, especially when five or more flowers at the same node in two soybean cultivars (Glycine max L. Merr. namely, Heinong35 and Kennong18). The flowers on sub-raceme frequently fail to develop into pods. Compared with the flowers on main-raceme, that on sub-raceme maybe have weak competitive ability to assimilation. Most flowers on bottom branches successfully develop into pods due to the strong supply of assimilation from the leaves of branches. Light enrichment significantly increase the number of flowers or pods across the main axis. However, the rate of flower abortion is still relatively high by observing the distribution curves of flowers or pods under light enrichment. Soybean plant has the characteristic of excessive flower production and flower abscission is more likely to be expression of biological adaptability.
大豆单株水平的开花过程是一个连续的动态系统。一朵花能否存活到成熟取决于它的生长位置和起始时间。通过田间试验，分析了开花不同步对单花存活的影响以及花或荚分布对光富集的响应。我们的数据表明，早期花(1-5)肯定能存活并发育成成熟的荚果，因为它们没有遇到来自其他花或荚果的激烈的同化物竞争。在两个大豆品种(Glycine max L. Merr.)中，当5朵或更多的花在同一节时，后期花通常容易流产。即河农35号和垦农18号)。亚总状花序上的花经常不能发育成豆荚。与主总状花序上的花相比，亚总状花序上的花可能具有较弱的同化竞争能力。底部树枝上的花大多能成功地发育成豆荚，这是由于树枝上的叶子提供了强大的同化作用。光富集显著增加了主轴上的花或荚的数量。但通过观察光富集条件下花或荚果的分布曲线，花的败育率仍然较高。大豆植物具有多花的特点，花脱落更可能是生物适应性的表达。
{"title":"Light Enrichment, Flowering Asynchrony and Reproduction Success in Two Field-Grown Soybeans in Northern China","authors":"Bing Liu, De-Ning Qu, Jianliang Liu","doi":"10.18805/LR-510","DOIUrl":"https://doi.org/10.18805/LR-510","url":null,"abstract":"The flowering process at single plant level in soybean is a continuous dynamic system. Whether a flower can survive to mature depends on where it is located and when it is initiated. A field experiment was conducted to analyse the effect of flowering asynchrony on single flower survival and response of flowers or pods distribution to light enrichment. Our data suggest that early flowers (number 1-5) definitely survive and develop into mature pod because they don’t encounter fierce assimilates competition from other flowers or pods. Later flowers are usually prone to abortion, especially when five or more flowers at the same node in two soybean cultivars (Glycine max L. Merr. namely, Heinong35 and Kennong18). The flowers on sub-raceme frequently fail to develop into pods. Compared with the flowers on main-raceme, that on sub-raceme maybe have weak competitive ability to assimilation. Most flowers on bottom branches successfully develop into pods due to the strong supply of assimilation from the leaves of branches. Light enrichment significantly increase the number of flowers or pods across the main axis. However, the rate of flower abortion is still relatively high by observing the distribution curves of flowers or pods under light enrichment. Soybean plant has the characteristic of excessive flower production and flower abscission is more likely to be expression of biological adaptability.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"26 1","pages":"241-246"},"PeriodicalIF":0.8,"publicationDate":"2020-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86099538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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