F. Nabi, A. Chaker-Haddadj, M. Chebaani, A. Ghalem, Samira Mebdoua, S. Ounane
The study was carried out to assess the effect of seed priming to enhance salt tolerance in Algerian Maghreb Cowpea. Seeds of two cowpea landraces (A18 and TZ2) were soaked for 4 h at 25oC in the dark in distilled water (hydropriming) or 80 mM of NaCl, 80 mM of CaSO4 or 80 mM of CaCl2 (halopriming) separately. Untreated seeds were taken as control (unprimed seeds). Seeds were germinated under three salinity levels (0 (distilled water), 85 mM and 170 mM of NaCl). In general, germination traits of cowpea landraces decreased with increased salinity levels, however, seed priming significantly increased germination percentage, speed of germination, final germination percentage, radical length, hypocotyl length, epicotyl lengh and induced better vigour index under saline and non-saline conditions as compared to the unprimed seed. Hydropriming, or halopriming with NaCl 80 mM or CaSO4 80 mM proved to be effective methods.
本研究旨在评价种子灌浆提高阿尔及利亚马格里布豇豆耐盐性的效果。将两个地方豇豆品种(A18和TZ2)的种子分别用蒸馏水(加氢)或80 mM NaCl、80 mM CaSO4或80 mM CaCl2(卤化)在25℃的黑暗条件下浸泡4 h。以未处理种子为对照(未处理种子)。种子在0(蒸馏水)、85 mM和170 mM NaCl 3种盐度水平下发芽。总体而言，随着盐度的增加，豇豆地方品种的萌发性状降低，但在盐水和非盐水条件下，种子萌发率、萌发速度、最终萌发率、胚根长度、下胚轴长度、上胚轴长度均显著高于无盐水条件下的种子，并诱导出更好的活力指数。经证明，加氢或盐浸(NaCl 80 mM或CaSO4 80 mM)是有效的方法。
{"title":"Influence of Seed Priming on Early Stages Growth of Cowpea [Vigna unguiculata (L.)Walp.] Grown under Salt Stress Conditions","authors":"F. Nabi, A. Chaker-Haddadj, M. Chebaani, A. Ghalem, Samira Mebdoua, S. Ounane","doi":"10.18805/LR-504","DOIUrl":"https://doi.org/10.18805/LR-504","url":null,"abstract":"The study was carried out to assess the effect of seed priming to enhance salt tolerance in Algerian Maghreb Cowpea. Seeds of two cowpea landraces (A18 and TZ2) were soaked for 4 h at 25oC in the dark in distilled water (hydropriming) or 80 mM of NaCl, 80 mM of CaSO4 or 80 mM of CaCl2 (halopriming) separately. Untreated seeds were taken as control (unprimed seeds). Seeds were germinated under three salinity levels (0 (distilled water), 85 mM and 170 mM of NaCl). In general, germination traits of cowpea landraces decreased with increased salinity levels, however, seed priming significantly increased germination percentage, speed of germination, final germination percentage, radical length, hypocotyl length, epicotyl lengh and induced better vigour index under saline and non-saline conditions as compared to the unprimed seed. Hydropriming, or halopriming with NaCl 80 mM or CaSO4 80 mM proved to be effective methods.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2020-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75936788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dugganna Girija, P. S. Panchami, P. E. Jose, T. Saeed, S. Nair
In the present study, eight rhizobial strains (RH-1 to RH-8) were isolated from root nodules of cowpea which grows in acidic soils of Wayanad. They were characterized based on morphological, physiological, biochemical and molecular characteristics. The results on plant growth promoting traits showed all isolates positive to IAA production, four positive to ammonia production and five positive for in vitro siderophore production. Three isolates exhibited antagonistic activity and none of them showed volatile cyanogen production. The 16S rRNA gene sequence analysis revealed all the isolates to show similarity to Rhizobium sp. While rhizobia generally have a pH range of 6.5–7.5 for optimum growth, the isolates RH-1. RH-2, RH-4 and RH-8 grew in a liquid yeast extract-mannitol agar medium at pH-4, suggesting acid tolerance in the strain of Rhizobium. The isolates were authenticated for their nodulation and growth promotion of cowpea in a paper cup experiment and four isolates (RH-2, RH-3, RH-4 and RH-5) were evaluated for their growth promotion in cowpea under field condition. It was observed that inoculation of Rhizobium increased nodulation, growth parameters and yield of cowpea compared to uninoculated control. This study showed that these rhizobial isolates with plant growth promoting traits can be used to promote the growth and yield of cow peain acidic soils of Kerala.
{"title":"Isolation and characterization of native cowpea rhizobia from Wayanad India","authors":"Dugganna Girija, P. S. Panchami, P. E. Jose, T. Saeed, S. Nair","doi":"10.18805/LR-3951","DOIUrl":"https://doi.org/10.18805/LR-3951","url":null,"abstract":"In the present study, eight rhizobial strains (RH-1 to RH-8) were isolated from root nodules of cowpea which grows in acidic soils of Wayanad. They were characterized based on morphological, physiological, biochemical and molecular characteristics. The results on plant growth promoting traits showed all isolates positive to IAA production, four positive to ammonia production and five positive for in vitro siderophore production. Three isolates exhibited antagonistic activity and none of them showed volatile cyanogen production. The 16S rRNA gene sequence analysis revealed all the isolates to show similarity to Rhizobium sp. While rhizobia generally have a pH range of 6.5–7.5 for optimum growth, the isolates RH-1. RH-2, RH-4 and RH-8 grew in a liquid yeast extract-mannitol agar medium at pH-4, suggesting acid tolerance in the strain of Rhizobium. The isolates were authenticated for their nodulation and growth promotion of cowpea in a paper cup experiment and four isolates (RH-2, RH-3, RH-4 and RH-5) were evaluated for their growth promotion in cowpea under field condition. It was observed that inoculation of Rhizobium increased nodulation, growth parameters and yield of cowpea compared to uninoculated control. This study showed that these rhizobial isolates with plant growth promoting traits can be used to promote the growth and yield of cow peain acidic soils of Kerala.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89191043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chloroplasts are involved in many cellular processes and play an important role in plant stress response. In this study, the effect of drought on different physiological characteristics and the isolation of chloroplasts from leaves of drought-stressed and control plants of common bean (Phaseolus vulgaris L.) are reported. Two cultivars differing in the response to drought were analysed-Tiber and more sensitive Starozagorski eern. The results of relative water content showed that plants were under mild stress conditions after six days without watering. Negative effects of drought stress on the photosynthetic rate and chlorophyll fluorescence parameters of both cultivars were observed. The yield of isolated chloroplasts in control samples of both cultivars was at least three times higher compared to drought-stressed samples. The intactness of the isolated chloroplasts was checked and used for protein isolation. The results of the study suggested that even mild drought stress strongly affected the isolation of intact chloroplasts. Still, intact chloroplasts were enriched and were suitable for downstream proteomic analysis.
{"title":"Impact of drought stress on physiological characteristics and isolation of chloroplasts in common bean (Phaseolus vulgaris L.)","authors":"T. Zadražnik, J. Šuštar-Vozlič","doi":"10.18805/LR-455","DOIUrl":"https://doi.org/10.18805/LR-455","url":null,"abstract":"Chloroplasts are involved in many cellular processes and play an important role in plant stress response. In this study, the effect of drought on different physiological characteristics and the isolation of chloroplasts from leaves of drought-stressed and control plants of common bean (Phaseolus vulgaris L.) are reported. Two cultivars differing in the response to drought were analysed-Tiber and more sensitive Starozagorski eern. The results of relative water content showed that plants were under mild stress conditions after six days without watering. Negative effects of drought stress on the photosynthetic rate and chlorophyll fluorescence parameters of both cultivars were observed. The yield of isolated chloroplasts in control samples of both cultivars was at least three times higher compared to drought-stressed samples. The intactness of the isolated chloroplasts was checked and used for protein isolation. The results of the study suggested that even mild drought stress strongly affected the isolation of intact chloroplasts. Still, intact chloroplasts were enriched and were suitable for downstream proteomic analysis.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82385832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A pot culture experiment was conducted at School of Natural Resource Management, College of Post Graduate Studies in Agricultural Sciences, CAU, Umiam, Meghalaya to assess the temporal availability of phosphorus and sulphur in acid Inceptisol of Meghalaya as influenced by graded application of P and S under black gram (cv. SBC-47) production. The treatment consisted of four levels of P (20, 40, 60 and 80 mg P kg-1 soil) and four levels of S (10, 20, 30 and 40 mg S kg-1 soil). The experiment was laid out in factorial completely randomized design with three replications during kharif season of 2016. The experimental soil was deficient in available phosphorus (13.85 kg ha-1) and available sulphur (15.91 kg ha-1). The results revealed that combined application of P and S had significant effects on seed and stover yield of black gram. The optimum seed and stover yield were recorded with combined application of 60 mg kg-1 soil and 30 mg kg-1 soil, respectively which was statistically at par with combined application of 80 mg P kg-1 soil and 40 mg S kg-1 soil. The assessment of temporal availability of phosphorus and sulphur at 20, 40 DAS and after harvesting of black gram in acid Inceptisol of Meghalaya indicated that available phosphorus in soil increased with each successive increasing level of P application, however it showed decreasing trend with time intervals of 40 DAS and after harvesting of black gram at each successive dose of 40, 60 and 80 mg P kg-1 soil over 20 mg P kg-1 soil in the presence of S fertilization. Similarly, available sulphur in soil also increased with each successive increasing level of S application, whereas it also showed decreasing trend with time intervals of 40 DAS and after harvesting of black gram at each successive dose of 20, 30 and 40 mg S kg-1 soil over control as 10 mg S kg-1 soil in the presence of P fertilization.
在梅加拉亚邦农业大学农业科学研究生院自然资源管理学院进行盆栽试验，评估黑克(cv)下磷和S分级施用对梅加拉亚邦酸性土壤中磷和硫的时间有效性的影响。SBC-47)生产。处理包括4个磷水平(20、40、60和80 mg P kg-1土壤)和4个S水平(10、20、30和40 mg S kg-1土壤)。试验采用因子完全随机设计，3个重复，于2016年秋收季节进行。试验土壤缺乏有效磷(13.85 kg ha-1)和有效硫(15.91 kg ha-1)。结果表明，磷、硫配施对黑克兰种子和秸秆产量均有显著影响。60 mg kg-1土壤和30 mg kg-1土壤配施的种子和秸秆产量最佳，与80 mg P kg-1土壤和40 mg S kg-1土壤配施的产量在统计学上相当。时间可用性的评估磷和硫在20、40 DAS和收获后的黑克酸始成土的土壤有效磷梅加拉亚邦表示,增加每个连续的P的含量增加应用程序,但是它显示下降趋势的时间间隔40 DAS和收获后在每个连续黑克剂量的40岁,60和80毫克P公斤土壤P超过20毫克公斤土壤的施肥。土壤有效硫也随施磷水平的逐次增加而增加，而在20、30和40 mg S kg-1土壤中，土壤有效硫也随时间间隔为40 DAS和黑克收获后呈下降趋势，在施磷条件下，土壤有效硫与10 mg S kg-1土壤相比呈下降趋势。
{"title":"Temporal availability of phosphorus and sulphur in acid Inceptisol as influenced by graded application of P and S under black gram (Vigna mungo L. Hepper) production","authors":"B. Tamang, S. Swami","doi":"10.18805/LR-4127","DOIUrl":"https://doi.org/10.18805/LR-4127","url":null,"abstract":"A pot culture experiment was conducted at School of Natural Resource Management, College of Post Graduate Studies in Agricultural Sciences, CAU, Umiam, Meghalaya to assess the temporal availability of phosphorus and sulphur in acid Inceptisol of Meghalaya as influenced by graded application of P and S under black gram (cv. SBC-47) production. The treatment consisted of four levels of P (20, 40, 60 and 80 mg P kg-1 soil) and four levels of S (10, 20, 30 and 40 mg S kg-1 soil). The experiment was laid out in factorial completely randomized design with three replications during kharif season of 2016. The experimental soil was deficient in available phosphorus (13.85 kg ha-1) and available sulphur (15.91 kg ha-1). The results revealed that combined application of P and S had significant effects on seed and stover yield of black gram. The optimum seed and stover yield were recorded with combined application of 60 mg kg-1 soil and 30 mg kg-1 soil, respectively which was statistically at par with combined application of 80 mg P kg-1 soil and 40 mg S kg-1 soil. The assessment of temporal availability of phosphorus and sulphur at 20, 40 DAS and after harvesting of black gram in acid Inceptisol of Meghalaya indicated that available phosphorus in soil increased with each successive increasing level of P application, however it showed decreasing trend with time intervals of 40 DAS and after harvesting of black gram at each successive dose of 40, 60 and 80 mg P kg-1 soil over 20 mg P kg-1 soil in the presence of S fertilization. Similarly, available sulphur in soil also increased with each successive increasing level of S application, whereas it also showed decreasing trend with time intervals of 40 DAS and after harvesting of black gram at each successive dose of 20, 30 and 40 mg S kg-1 soil over control as 10 mg S kg-1 soil in the presence of P fertilization.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85852454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The knowledge of the extent of underlying diversity in the germplasm can be effectively employed to breed desirable cultivars and to challenge the consequences of the unprecedented biological, physical and chemical stresses of the future growing conditions. In the present study, total 26 geographically diverse French bean genotypes collected from different agro-climatic zones of Himachal Pradesh and two check varieties viz., Baspa and Jawala were grown under randomized complete block design with three replications for deciphering the extent of genetic diversity. The extent of variability was assessed using DUS morphological traits and PCR based approach. Analysis of variance for quantitative traits showed significant differences among all the genotypes for all the characters studied. The genotypes RL-19 and RL-20 gave higher seed yield and performed better for many of the horticultural traits over all the checks. Further, 15 RAPD primers were used for scoring polymorphism at the DNA level. The French bean landraces produced 79 PCR fragments, of which 59 were polymorphic with an average of 3.93 polymorphic fragments per primer. The overall percentage of polymorphism was recorded 74.62 per cent. The average Jaccard similarity index among genotypes ranged from 0.48 to 0.98, thereby showing sufficient genetic variability. Geographical affiliations and morphological associations were observed while studying the genotypic patterns within each group i.e. A, B, C and D. Such variability is desirable in breeding programs. Which also reveal the inherent capacity of the community as a selector, conservator and improver of the potential genetic resource for sustainable future.
{"title":"Studies on genetic diversity of French bean (Phaseolus vulgaris L.) landraces of Himachal Pradesh based on morphological traits and molecular markers","authors":"R. Kanwar, D. Mehta, Rajnish Sharma, R. Dogra","doi":"10.18805/LR-4089","DOIUrl":"https://doi.org/10.18805/LR-4089","url":null,"abstract":"The knowledge of the extent of underlying diversity in the germplasm can be effectively employed to breed desirable cultivars and to challenge the consequences of the unprecedented biological, physical and chemical stresses of the future growing conditions. In the present study, total 26 geographically diverse French bean genotypes collected from different agro-climatic zones of Himachal Pradesh and two check varieties viz., Baspa and Jawala were grown under randomized complete block design with three replications for deciphering the extent of genetic diversity. The extent of variability was assessed using DUS morphological traits and PCR based approach. Analysis of variance for quantitative traits showed significant differences among all the genotypes for all the characters studied. The genotypes RL-19 and RL-20 gave higher seed yield and performed better for many of the horticultural traits over all the checks. Further, 15 RAPD primers were used for scoring polymorphism at the DNA level. The French bean landraces produced 79 PCR fragments, of which 59 were polymorphic with an average of 3.93 polymorphic fragments per primer. The overall percentage of polymorphism was recorded 74.62 per cent. The average Jaccard similarity index among genotypes ranged from 0.48 to 0.98, thereby showing sufficient genetic variability. Geographical affiliations and morphological associations were observed while studying the genotypic patterns within each group i.e. A, B, C and D. Such variability is desirable in breeding programs. Which also reveal the inherent capacity of the community as a selector, conservator and improver of the potential genetic resource for sustainable future.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2019-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86316402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was designed to explore the ameliorative effect of exogenously applied 24-epibrassinolide (24-EBL) against salt stress in Vigna mungo (L.) Hepper. The plants were treated with four different levels of salinity (0, 8, 12 and 16 dSm-1) followed by the foliar spray of 24-EBL (0, 10-5, 10-7 and 10-9 M). The results showed that 24-EBL could alleviate the adverse effects of salinity by improving the growth parameters of 35 days old plants. The same also stimulated the leaf enzymatic antioxidant activity in salt-treated plants. The findings of the present study revealed that application of 24-EBL ameliorated the salt stress in black gram plants.
{"title":"Improvement in salt tolerance of Vigna mungo(L.) Hepper by exogenously applied 24-epibrassinolide","authors":"Sombir Singh, S. Jakhar, Suhana Rao","doi":"10.18805/LR-4019","DOIUrl":"https://doi.org/10.18805/LR-4019","url":null,"abstract":"This study was designed to explore the ameliorative effect of exogenously applied 24-epibrassinolide (24-EBL) against salt stress in Vigna mungo (L.) Hepper. The plants were treated with four different levels of salinity (0, 8, 12 and 16 dSm-1) followed by the foliar spray of 24-EBL (0, 10-5, 10-7 and 10-9 M). The results showed that 24-EBL could alleviate the adverse effects of salinity by improving the growth parameters of 35 days old plants. The same also stimulated the leaf enzymatic antioxidant activity in salt-treated plants. The findings of the present study revealed that application of 24-EBL ameliorated the salt stress in black gram plants.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2019-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89630226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The storage experiment was carried out at Seed Quality and Research Laboratory, Seed Unit (National Seed Project), University of Agricultural Sciences, Dharwad from March 2017 to March 2018 to study the influence of seed treatment with botanicals and chemicals on storability of Kabuli chickpea varieties. Seed quality parameters were significantly influenced among varieties and seed treatment with botanicals and chemicals. Among the treatments, seed treatment with Sprint (Mancozeb 50 % + Carbendazim 25 % WS) @ 2 g per kg of seed or Vitavax power @ 3 g/kg of seed or Sweet flag rhizome @ 10 g/kg of seed maintained seed quality for more than ten months during storage. However, Sweet flag rhizome @ 10 g/kg of seed can be used as alternative to chemicals which is safe, eco-friendly and economically feasible for maintaining seed quality for organic seed growers/producers and seed companies.
{"title":"Influence of seed treatment with botanicals and chemicals on storability of Kabuli chickpea (Cicer arietinum L.) varieties","authors":"P. Shinde, R. Hunje","doi":"10.18805/LR-4101","DOIUrl":"https://doi.org/10.18805/LR-4101","url":null,"abstract":"The storage experiment was carried out at Seed Quality and Research Laboratory, Seed Unit (National Seed Project), University of Agricultural Sciences, Dharwad from March 2017 to March 2018 to study the influence of seed treatment with botanicals and chemicals on storability of Kabuli chickpea varieties. Seed quality parameters were significantly influenced among varieties and seed treatment with botanicals and chemicals. Among the treatments, seed treatment with Sprint (Mancozeb 50 % + Carbendazim 25 % WS) @ 2 g per kg of seed or Vitavax power @ 3 g/kg of seed or Sweet flag rhizome @ 10 g/kg of seed maintained seed quality for more than ten months during storage. However, Sweet flag rhizome @ 10 g/kg of seed can be used as alternative to chemicals which is safe, eco-friendly and economically feasible for maintaining seed quality for organic seed growers/producers and seed companies.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73456879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cowpea is a drought tolerant food legume but yields are generally low in South Africa due to non-availability of improved varieties. The current study was undertaken to evaluate agronomic performance of cowpea germplasm and select the candidate accessions for breeding population and cultivation by small-scale farmers in South Africa. A randomized complete block design with three replications was used for the experiment. Data were collected on the agronomic traits including days to 50% maturity, days to 95% maturity, hundred seed weight, leaf area index, number of branches, number of internodes, number of seeds per plant, plant height, number of pods per plant, pod length, dry biomass weight per plant, grain yield per plant and grain yield per hectare. Highly significant differences were observed among the accessions for all traits. The grain yield per hectare was correlated positively and significantly with number of pods per plant and grain yield per plant showing a scope for simultaneous improvement of yield and yield related traits. Principal Component biplot revealed that accessions Acc2024, Acc5352, Acc2355, Acc4565, Glenda and L-cwp3 were the most genetically distinct accessions and can serve as potential candidate parental lines for hybridization to achieving transgressive segregation population in breeding programmes.
{"title":"Agronomic performance and genetic variability of cowpea (Vigna unguiculata) Accessions","authors":"D. K. Nkoana, A. Gerrano, E. Gwata","doi":"10.18805/LR-450","DOIUrl":"https://doi.org/10.18805/LR-450","url":null,"abstract":"Cowpea is a drought tolerant food legume but yields are generally low in South Africa due to non-availability of improved varieties. The current study was undertaken to evaluate agronomic performance of cowpea germplasm and select the candidate accessions for breeding population and cultivation by small-scale farmers in South Africa. A randomized complete block design with three replications was used for the experiment. Data were collected on the agronomic traits including days to 50% maturity, days to 95% maturity, hundred seed weight, leaf area index, number of branches, number of internodes, number of seeds per plant, plant height, number of pods per plant, pod length, dry biomass weight per plant, grain yield per plant and grain yield per hectare. Highly significant differences were observed among the accessions for all traits. The grain yield per hectare was correlated positively and significantly with number of pods per plant and grain yield per plant showing a scope for simultaneous improvement of yield and yield related traits. Principal Component biplot revealed that accessions Acc2024, Acc5352, Acc2355, Acc4565, Glenda and L-cwp3 were the most genetically distinct accessions and can serve as potential candidate parental lines for hybridization to achieving transgressive segregation population in breeding programmes.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78478623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Pandey, A. Singh, P. Singh, R. P. Vyas, Ravish Kumar Singh, H. Bhandari
Tris- and water-soluble seed protein profiling was used to characterize, identify and differentiate 13 genotypes of pigeonpea via SDS–PAGE based electrophoresis of seed protein. Electropherograms, RM-value and UPGMA based cluster (dendrograms) analysis was used to analyze tris- and water-soluble protein banding patterns of these genotypes. It revealed that RM-value of protein bands have differed from all the genotypes for both soluble proteins. Tris-soluble protein banding pattern exhibited 17 bands including 12-15 variate of RM-value (0.083 to 0.98) and two common bands with RM-value 0.083 and 0.78. However, water-soluble protein banding pattern revealed the presence of 16 bands including 8-12 variate of RM-value (0.16 to 0.95) with four common bands of RM-value 0.43, 048, 0.23 and 0.65, respectively. UPGMA cluster analysis was used to group the 13 genotypes into six distinct clusters. The banding pattern of tris-soluble protein was found to be more distinct than water-soluble protein. The result demonstrated that the electrophoretic profile of tris-soluble protein through SDS-PAGE was more effective than the water-soluble protein.
{"title":"Characterization and identification of pigeonpea [Cajanus cajan (L.) Millsp.] genotypes based on quality of seed protein","authors":"A. Pandey, A. Singh, P. Singh, R. P. Vyas, Ravish Kumar Singh, H. Bhandari","doi":"10.18805/LR-3696","DOIUrl":"https://doi.org/10.18805/LR-3696","url":null,"abstract":"Tris- and water-soluble seed protein profiling was used to characterize, identify and differentiate 13 genotypes of pigeonpea via SDS–PAGE based electrophoresis of seed protein. Electropherograms, RM-value and UPGMA based cluster (dendrograms) analysis was used to analyze tris- and water-soluble protein banding patterns of these genotypes. It revealed that RM-value of protein bands have differed from all the genotypes for both soluble proteins. Tris-soluble protein banding pattern exhibited 17 bands including 12-15 variate of RM-value (0.083 to 0.98) and two common bands with RM-value 0.083 and 0.78. However, water-soluble protein banding pattern revealed the presence of 16 bands including 8-12 variate of RM-value (0.16 to 0.95) with four common bands of RM-value 0.43, 048, 0.23 and 0.65, respectively. UPGMA cluster analysis was used to group the 13 genotypes into six distinct clusters. The banding pattern of tris-soluble protein was found to be more distinct than water-soluble protein. The result demonstrated that the electrophoretic profile of tris-soluble protein through SDS-PAGE was more effective than the water-soluble protein.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81128618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Öten, C. Erdurmuş, S. Ki̇remi̇tci̇, Celal Yücel
Two experiments each following randomized complete block design with three replications were conducted simultaneously in Antalya and Adana locations in 2015-2016 growing season with 9 different common vetch lines and 3 cultivars to compare their yield and quality performance with respect to 17 different characters and also to find the relationships between them. The results revealed that interlocational difference of mean values for the characters were more pronounced than those within location. From yield and quality parameters investigated in this study significant differences could be noticed for the morphological characters at large and a for a few of the quality characters. Although no line could be identified to be superior for a good number of characters, line 2 has been recognized to be superior.
{"title":"Influence of locations under same climatic zone on some common vetch lines (Vicia sativa L.)","authors":"M. Öten, C. Erdurmuş, S. Ki̇remi̇tci̇, Celal Yücel","doi":"10.18805/LR-476","DOIUrl":"https://doi.org/10.18805/LR-476","url":null,"abstract":"Two experiments each following randomized complete block design with three replications were conducted simultaneously in Antalya and Adana locations in 2015-2016 growing season with 9 different common vetch lines and 3 cultivars to compare their yield and quality performance with respect to 17 different characters and also to find the relationships between them. The results revealed that interlocational difference of mean values for the characters were more pronounced than those within location. From yield and quality parameters investigated in this study significant differences could be noticed for the morphological characters at large and a for a few of the quality characters. Although no line could be identified to be superior for a good number of characters, line 2 has been recognized to be superior.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2019-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79641284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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