J. F. Zamora-Natera, R. Rodríguez-Macías, Eduardo Salcedo-Pérez, P. García-López, L. Barrientos-Ramírez, J. J. Vargas-Radillo, C. Soto-Velasco, M. Ruiz-López
The study of new legumes species in terms of yield and quality of forage under cultivation conditions is essential to improving the productivity of animal production. We evaluated the yield and nutritional quality of forage under irrigation conditions for three wild lupin species from Mexico (L. exaltatus, L. mexicanus, and L. rotundiflorus) at different stages of maturity. The experiment was conducted from November 2016 to March 2017 in Jalisco, Mexico. In general, the dry matter (DM) in all species increased with advancing maturity, but L. exaltatus presented the best production of forage 15, 143 kg/ha-1 DM, followed by L. mexicanus (9, 140 kg/ha-1 DM) on the third sampling date. The low forage yield in L. rotundiflorus was compensated by a higher average protein content (171.2 g/kg). The highest P and K content was recorded in L. rotundiflorus, whereas Ca and Mg were higher in the forage of L. mexicanus.
{"title":"Forage potential of three wild species of genus Lupinus (Leguminosae) from Mexico","authors":"J. F. Zamora-Natera, R. Rodríguez-Macías, Eduardo Salcedo-Pérez, P. García-López, L. Barrientos-Ramírez, J. J. Vargas-Radillo, C. Soto-Velasco, M. Ruiz-López","doi":"10.18805/LR-453","DOIUrl":"https://doi.org/10.18805/LR-453","url":null,"abstract":"The study of new legumes species in terms of yield and quality of forage under cultivation conditions is essential to improving the productivity of animal production. We evaluated the yield and nutritional quality of forage under irrigation conditions for three wild lupin species from Mexico (L. exaltatus, L. mexicanus, and L. rotundiflorus) at different stages of maturity. The experiment was conducted from November 2016 to March 2017 in Jalisco, Mexico. In general, the dry matter (DM) in all species increased with advancing maturity, but L. exaltatus presented the best production of forage 15, 143 kg/ha-1 DM, followed by L. mexicanus (9, 140 kg/ha-1 DM) on the third sampling date. The low forage yield in L. rotundiflorus was compensated by a higher average protein content (171.2 g/kg). The highest P and K content was recorded in L. rotundiflorus, whereas Ca and Mg were higher in the forage of L. mexicanus.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"54 1","pages":"93-98"},"PeriodicalIF":0.8,"publicationDate":"2019-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75006187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Divya Choudhary, Gaurav Agarwal, Hui Wang, M. Pandey, A. Culbreath, R. Varshney, B. Guo
Recent polyploidation of peanut genome and geographical isolation has rendered peanut to be a highly monomorphic species. Due to its narrow genetic base, cultivated peanut has been susceptible to various diseases, causing economic loss to farmers. Availability of only a few disease resistance sources in cultivated peanut has resulted in limited success using the conventional breeding practices. Also, scarcity of markers has been the major limiting factor to precisely identify the disease resistance genomic regions. Recentidentification of largenumber ofmolecular markers using advancedgenomic resources and high throughput sequencing technologies has and will continue to assist in improvement of peanut diversity and breeding. This review gives an update on recent discovery of molecular markers associated with major diseases and the available genomic resources in peanut.
{"title":"Molecular markers and genomic resources for disease resistance in peanut-A review","authors":"Divya Choudhary, Gaurav Agarwal, Hui Wang, M. Pandey, A. Culbreath, R. Varshney, B. Guo","doi":"10.18805/LR-409","DOIUrl":"https://doi.org/10.18805/LR-409","url":null,"abstract":"Recent polyploidation of peanut genome and geographical isolation has rendered peanut to be a highly monomorphic species. Due to its narrow genetic base, cultivated peanut has been susceptible to various diseases, causing economic loss to farmers. Availability of only a few disease resistance sources in cultivated peanut has resulted in limited success using the conventional breeding practices. Also, scarcity of markers has been the major limiting factor to precisely identify the disease resistance genomic regions. Recentidentification of largenumber ofmolecular markers using advancedgenomic resources and high throughput sequencing technologies has and will continue to assist in improvement of peanut diversity and breeding. This review gives an update on recent discovery of molecular markers associated with major diseases and the available genomic resources in peanut.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"434 1","pages":"137-144"},"PeriodicalIF":0.8,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85532629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The evaluation and characterization of germplasm of mungbean are considered to be essential prerequisites for a rational use of its genetic resources. Accordingly in the present study, genetic diversity in 18 mungbean genotypes was assessed using a panel of 40 microsatellite based primer pairs. Out of 40 primers initially tested, only 24 showed distinct polymorphism and consequently only these primers were utilized for the purpose of genome profiling of the entries. Altogether, 183 allelic variants were detected with an average of 4.95 polymorphic fragment per primer. The number of amplified loci varied from 4 (CEDG 15) to a maximum of 12 (CEDG 92 and CEDG 172) with an average of 4.95 polymorphic fragment per primer. The polymorphic information content (PIC) ranged from 0.440 to 0.928 with an average of 0.822 per SSR primer pair. The lowest polymorphism percent was observed for primer CEDGAG001 (16.67) while the primer CEDG 154 gave the maximum polymorphism percent (63.64). The primer pairs CEDG 008, CEDG 068 and CEDG 154 among the total primer pairs were found to be highly informative. Dice's similarity coefficient ranged from 0.03 to 0.58. Dendrogram based on SSR data grouped the mungbean genotypes into seven clusters. The pattern of clustering was also reiterated by the results of principal component analysis (PCA). The SSR primer based analysis allowed unique and unambiguous genotyping of the entries. The genetic diversity observed in the present study could be useful to know the phylogenetic links among the cultivars and for the selection of suitable parents to be further used in breeding programmes and genetic mapping studies.
{"title":"SSR marker based profiling and diversity analysis of mungbean [Vigna radiata (L.) Wilczek] genotypes","authors":"S. Suman, B. Rani, V. Sharma, H. Kumar, V. Shahi","doi":"10.18805/LR-3918","DOIUrl":"https://doi.org/10.18805/LR-3918","url":null,"abstract":"The evaluation and characterization of germplasm of mungbean are considered to be essential prerequisites for a rational use of its genetic resources. Accordingly in the present study, genetic diversity in 18 mungbean genotypes was assessed using a panel of 40 microsatellite based primer pairs. Out of 40 primers initially tested, only 24 showed distinct polymorphism and consequently only these primers were utilized for the purpose of genome profiling of the entries. Altogether, 183 allelic variants were detected with an average of 4.95 polymorphic fragment per primer. The number of amplified loci varied from 4 (CEDG 15) to a maximum of 12 (CEDG 92 and CEDG 172) with an average of 4.95 polymorphic fragment per primer. The polymorphic information content (PIC) ranged from 0.440 to 0.928 with an average of 0.822 per SSR primer pair. The lowest polymorphism percent was observed for primer CEDGAG001 (16.67) while the primer CEDG 154 gave the maximum polymorphism percent (63.64). The primer pairs CEDG 008, CEDG 068 and CEDG 154 among the total primer pairs were found to be highly informative. Dice's similarity coefficient ranged from 0.03 to 0.58. Dendrogram based on SSR data grouped the mungbean genotypes into seven clusters. The pattern of clustering was also reiterated by the results of principal component analysis (PCA). The SSR primer based analysis allowed unique and unambiguous genotyping of the entries. The genetic diversity observed in the present study could be useful to know the phylogenetic links among the cultivars and for the selection of suitable parents to be further used in breeding programmes and genetic mapping studies.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"61 1","pages":"585-594"},"PeriodicalIF":0.8,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83899244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sunflower is an important oilseed crop in India. One of the major diseases is collar rot caused by Sclerotium rolfsii (Sacc). This study was conducted during 2014–15 and 2015–16 under in-vitro condition. To see the effect of soil temperature and soil moisture, the S. rolfsii (Sacc). was grow at different temperature and at different moisture level. Results showed that among the different soil pH tested, pH5.7 was the best for colonization on wheat seeds 61.67% followed by 6.1(58.33%). In case of soil temperature most preferable temperature for mycelial grow of the pathogen was 30°C. The pathogen grown best in 30% soil moisture content followed by 25%. Variations were observed in colony morphology, mycelial growth, sclerotial size, colour and number of sclerotia. The number of sclerotia in different isolates varied from 352 (WBSR4) to 411 (WBSR9). Twenty five combinations showed compatible reaction out of 36 pairings in mycelial compatibility studies.
{"title":"Effect of variability and edaphological characteristics on growth of Sclerotium rolfsii (Sacc) causing collar rot disease of sunflower in coastal region of West Bengal, India","authors":"A. Maji, R. Nath, Deepak Singh, P. K. Garain","doi":"10.18805/LR-3922","DOIUrl":"https://doi.org/10.18805/LR-3922","url":null,"abstract":"Sunflower is an important oilseed crop in India. One of the major diseases is collar rot caused by Sclerotium rolfsii (Sacc). This study was conducted during 2014–15 and 2015–16 under in-vitro condition. To see the effect of soil temperature and soil moisture, the S. rolfsii (Sacc). was grow at different temperature and at different moisture level. Results showed that among the different soil pH tested, pH5.7 was the best for colonization on wheat seeds 61.67% followed by 6.1(58.33%). In case of soil temperature most preferable temperature for mycelial grow of the pathogen was 30°C. The pathogen grown best in 30% soil moisture content followed by 25%. Variations were observed in colony morphology, mycelial growth, sclerotial size, colour and number of sclerotia. The number of sclerotia in different isolates varied from 352 (WBSR4) to 411 (WBSR9). Twenty five combinations showed compatible reaction out of 36 pairings in mycelial compatibility studies.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"179 1","pages":"705-709"},"PeriodicalIF":0.8,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73009961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chickpea varieties (BG-256 and CSG-8962) were subjected to salt stress (50mM, 100mM and 150mM of NaCl) in a pot experiment. Untreated plants served as control. Plants were analyzed from 20 DAS up to 60 DAS at ten-day interval. Salt stress significantly reduced growth parameters like biomass, net assimilation rate, relative water content and biochemical parameters viz., total nitrogen and protein content of both the varieties. However, decrement was more pronounced in sensitive (BG-256) than in tolerant (CSG-8962) variety. Proline content increased with increase in salt exposure in both the varieties. SDS-PAGE of the protein reveal large amount of protein degradation in plants treated with high concentration of salt.
{"title":"Biochemical performance and protein profile of sensitive and tolerant varieties of chickpea under salinity","authors":"Rajnish Kumar, S. Shahi, Malvika Srivastava","doi":"10.18805/LR-4045","DOIUrl":"https://doi.org/10.18805/LR-4045","url":null,"abstract":"Chickpea varieties (BG-256 and CSG-8962) were subjected to salt stress (50mM, 100mM and 150mM of NaCl) in a pot experiment. Untreated plants served as control. Plants were analyzed from 20 DAS up to 60 DAS at ten-day interval. Salt stress significantly reduced growth parameters like biomass, net assimilation rate, relative water content and biochemical parameters viz., total nitrogen and protein content of both the varieties. However, decrement was more pronounced in sensitive (BG-256) than in tolerant (CSG-8962) variety. Proline content increased with increase in salt exposure in both the varieties. SDS-PAGE of the protein reveal large amount of protein degradation in plants treated with high concentration of salt.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"19 1","pages":"634-640"},"PeriodicalIF":0.8,"publicationDate":"2018-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81618717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Pradhan, Shilpee Dhali, R. Sahoo, C. Pradhan, S. Mohanty
The present study described the influence of Bacillus amyloliquefaciens CTC12 (KT633845) and Burkholderia cepacia KHD08 (KT717633) in combination with inorganic P fertilizer (SSP) on the inorganic P fractions (Ca-P and non occluded Al-P and Fe-P) in an acid agricultural soil. Though not significant but the highest available phosphorus was found with the combined application of 75 per cent P as SSP and inoculum KHD08 at 75 days after sowing (DAS) and harvest. Non-occluded Al-P and Fe-P accounted for maximum inorganic P fraction. Plots receiving 100 per cent single super phosphate (SSP) either sole or in combination with PSB recorded maximum non occluded Al-P and Fe-P and Ca-P. Sole application of Bacillus amyloliquefaciens CTC12 or Burkholderia cepacia KHD08 reduced the concentration of non occluded Al-P and Fe-P as well as Ca-P. The application of P solubilizing bacteria least influenced soil pH and organic carbon. However, combined application of P solubilizing bacteria and single super phosphate positively influenced pod yield and kernel P uptake. The beneficial effect of these rhizo-bacteria can be effectively used as bioinoculants combined with lower doses of inorganic P fertilizer in problematic acid agricultural soils in order to enhance crop productivity and soil available P.
{"title":"Effect of P solubilizing bacteria and P fertilizer on inorganic P fractions of acid soil and its influence on P uptake in groundnut (Arachis hypogaea L)","authors":"M. Pradhan, Shilpee Dhali, R. Sahoo, C. Pradhan, S. Mohanty","doi":"10.18805/LR-3991","DOIUrl":"https://doi.org/10.18805/LR-3991","url":null,"abstract":"The present study described the influence of Bacillus amyloliquefaciens CTC12 (KT633845) and Burkholderia cepacia KHD08 (KT717633) in combination with inorganic P fertilizer (SSP) on the inorganic P fractions (Ca-P and non occluded Al-P and Fe-P) in an acid agricultural soil. Though not significant but the highest available phosphorus was found with the combined application of 75 per cent P as SSP and inoculum KHD08 at 75 days after sowing (DAS) and harvest. Non-occluded Al-P and Fe-P accounted for maximum inorganic P fraction. Plots receiving 100 per cent single super phosphate (SSP) either sole or in combination with PSB recorded maximum non occluded Al-P and Fe-P and Ca-P. Sole application of Bacillus amyloliquefaciens CTC12 or Burkholderia cepacia KHD08 reduced the concentration of non occluded Al-P and Fe-P as well as Ca-P. The application of P solubilizing bacteria least influenced soil pH and organic carbon. However, combined application of P solubilizing bacteria and single super phosphate positively influenced pod yield and kernel P uptake. The beneficial effect of these rhizo-bacteria can be effectively used as bioinoculants combined with lower doses of inorganic P fertilizer in problematic acid agricultural soils in order to enhance crop productivity and soil available P.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"118 1","pages":"694-698"},"PeriodicalIF":0.8,"publicationDate":"2018-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74847109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
India is the largest producer and consumer of pulses in the world, accounting for about 25% of global production, 27% of consumption and 34% of food use. More than two-third area and production has been obtained from the six states of India viz., Madhya Pradesh, Rajasthan, Maharashtra, Karnataka, Andhra Pradesh and Uttar Pradesh. India is leading importer and about 20% of the total pulses demands are met by imports only due to the stagnant production over the years. To reduce the demand-supply gap, government of India launched various programmes in pulses. However, prime attention is required to meet the food security challenges, especially in case of pulse production. These crops are only the option to cure the sick land which is ill by chemical based modern cultivation. In order to enhance and sustain the pulse productivity at high levels, the development and promotion of pulse production technology need greater attention so that technology is widely adopted by the majority of farmers. The paper addresses the present scenario, bottleneck and focus on the way forward for sustaining/improving pulses production in India.
{"title":"Present scenario, bottlenecks and expansion of pulse production in India: A review","authors":"U. Shukla, M. Mishra","doi":"10.18805/LR-3998","DOIUrl":"https://doi.org/10.18805/LR-3998","url":null,"abstract":"India is the largest producer and consumer of pulses in the world, accounting for about 25% of global production, 27% of consumption and 34% of food use. More than two-third area and production has been obtained from the six states of India viz., Madhya Pradesh, Rajasthan, Maharashtra, Karnataka, Andhra Pradesh and Uttar Pradesh. India is leading importer and about 20% of the total pulses demands are met by imports only due to the stagnant production over the years. To reduce the demand-supply gap, government of India launched various programmes in pulses. However, prime attention is required to meet the food security challenges, especially in case of pulse production. These crops are only the option to cure the sick land which is ill by chemical based modern cultivation. In order to enhance and sustain the pulse productivity at high levels, the development and promotion of pulse production technology need greater attention so that technology is widely adopted by the majority of farmers. The paper addresses the present scenario, bottleneck and focus on the way forward for sustaining/improving pulses production in India.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2018-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89794517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Using a quartz sand culture, comparisons were made between the T5 homozygous transgenic lines (HTLs) that overexpress StP5CS (GenBank accession number: JN606861) and their wild-type (WT) host cultivar to examine the differences in the growth and development traits, the concentrations of proline in vegetable soybean [Glycine max (L.) Merrill] under salt stress conditions. Moreover, the relative expression levels of two glutamine synthetase-related genes (GmGS1â1, GmGS1â2), two nodulation-related genes (GmENOD40-1, GmENOD40-2), and one leghemoglobin gene (GmLba) were also measured. The purpose of this research was to provide a theoretical basis for elucidating the mechanisms of nodulation and nitrogen fixation in the roots of transgenic plants under salt stress conditions. Compared with WT plants, the plant height and seed weight per plant of T5 HTLs significantly increased, and the contents of proline in various tissues of T5 HTLs were also significantly elevated. Quantitative real-time PCR (qRT-PCR) analysis indicated that the expression levels of GmGS1â1, GmGS1â2, GmENOD40-1, GmENOD40-2, and GmLba were significantly increased in T5 HTLs under salt stress conditions. These results indicate that the overexpression of StP5CS in T5 HTLs enhanced growth, nodulation and nitrogen fixation in transgenic vegetable soybean under salt stress conditions.
{"title":"Effects of StP5CS gene overexpression on nodulation and nitrogen fixation of vegetable soybean under salt stress conditions","authors":"X. Ren, D. Yu, Shouping Yang, J. Gai, Yuelin Zhu","doi":"10.18805/LR-386","DOIUrl":"https://doi.org/10.18805/LR-386","url":null,"abstract":"Using a quartz sand culture, comparisons were made between the T5 homozygous transgenic lines (HTLs) that overexpress StP5CS (GenBank accession number: JN606861) and their wild-type (WT) host cultivar to examine the differences in the growth and development traits, the concentrations of proline in vegetable soybean [Glycine max (L.) Merrill] under salt stress conditions. Moreover, the relative expression levels of two glutamine synthetase-related genes (GmGS1â1, GmGS1â2), two nodulation-related genes (GmENOD40-1, GmENOD40-2), and one leghemoglobin gene (GmLba) were also measured. The purpose of this research was to provide a theoretical basis for elucidating the mechanisms of nodulation and nitrogen fixation in the roots of transgenic plants under salt stress conditions. Compared with WT plants, the plant height and seed weight per plant of T5 HTLs significantly increased, and the contents of proline in various tissues of T5 HTLs were also significantly elevated. Quantitative real-time PCR (qRT-PCR) analysis indicated that the expression levels of GmGS1â1, GmGS1â2, GmENOD40-1, GmENOD40-2, and GmLba were significantly increased in T5 HTLs under salt stress conditions. These results indicate that the overexpression of StP5CS in T5 HTLs enhanced growth, nodulation and nitrogen fixation in transgenic vegetable soybean under salt stress conditions.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"75 1","pages":"675-680"},"PeriodicalIF":0.8,"publicationDate":"2018-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83299746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A total of 120 root-nodule bacteria from chickpea plants (Cicer arietinum L.) from six regions in Turkey were characterized using 71 phenotypes. Utilization of carbon and nitrogen sources, tolerance to salt stress, temperature and pH, resistance to antibiotics and heavy metals and ability to produce some enzymes, were assessed. 90% of the isolates produced mucously, circular, smooth-margined and watery to creamy colonies with 2–4 mm diameter after 1-3 days of growth. The isolates utilized different compounds as sole carbon and nitrogen sources, endured 2% salt, grew optimally at 25-35°C and pHs between 6-8, exhibited insensitivity to the heavy metals Zn, Hg, and Cu and the antibiotics kanamycin, streptomycin and tetracycline. Numerical analysis separated the isolates into three clusters at 25% similarity. Results revealed diversity among isolates and were consistent with previous findings on chickpea Mesorhizobia.
{"title":"Phenotypic characterization of indigenous rhizobia nodulating chickpea in Turkey reveals high diversity","authors":"M. Mahmoud, D. Mart, C. Can","doi":"10.18805/LR-430","DOIUrl":"https://doi.org/10.18805/LR-430","url":null,"abstract":"A total of 120 root-nodule bacteria from chickpea plants (Cicer arietinum L.) from six regions in Turkey were characterized using 71 phenotypes. Utilization of carbon and nitrogen sources, tolerance to salt stress, temperature and pH, resistance to antibiotics and heavy metals and ability to produce some enzymes, were assessed. 90% of the isolates produced mucously, circular, smooth-margined and watery to creamy colonies with 2–4 mm diameter after 1-3 days of growth. The isolates utilized different compounds as sole carbon and nitrogen sources, endured 2% salt, grew optimally at 25-35°C and pHs between 6-8, exhibited insensitivity to the heavy metals Zn, Hg, and Cu and the antibiotics kanamycin, streptomycin and tetracycline. Numerical analysis separated the isolates into three clusters at 25% similarity. Results revealed diversity among isolates and were consistent with previous findings on chickpea Mesorhizobia.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"15 1","pages":"379-384"},"PeriodicalIF":0.8,"publicationDate":"2018-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74908570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Jasrotia, K. S. Jadon, Surender Singh, M. Nataraja, G. Harish, R. Dutta, R. D. Padvi, S. Savaliya
Five different IPM modules were designed and tested for the management of insect-pests in groundnut including farmer practices during 2013 to 2016 at Junagadh, Gujarat. Data were recorded for the incidence of major sucking pests, i.e. thrips (Caliothrips indicus) and leaf hoppers (Balclutha hortensis). It was observed that synthesized IPM Modules significantly reduced insect-pest incidence on groundnut and enhanced the yield over farmers’ practices (FP). The population of thrips and leaf hoppers were recorded to be lowest in module T5 and varied from 0.46 to 1.09 thrips/plant and 0.47 to 4.0 leafhoppers/plant, respectively. The module T5 was significantly superior to Farmer Practice during both Kharif and Rabi-summer seasons.
{"title":"Development and validation of IPM modules against major sucking insect-pests of groundnut","authors":"P. Jasrotia, K. S. Jadon, Surender Singh, M. Nataraja, G. Harish, R. Dutta, R. D. Padvi, S. Savaliya","doi":"10.18805/LR-4013","DOIUrl":"https://doi.org/10.18805/LR-4013","url":null,"abstract":"Five different IPM modules were designed and tested for the management of insect-pests in groundnut including farmer practices during 2013 to 2016 at Junagadh, Gujarat. Data were recorded for the incidence of major sucking pests, i.e. thrips (Caliothrips indicus) and leaf hoppers (Balclutha hortensis). It was observed that synthesized IPM Modules significantly reduced insect-pest incidence on groundnut and enhanced the yield over farmers’ practices (FP). The population of thrips and leaf hoppers were recorded to be lowest in module T5 and varied from 0.46 to 1.09 thrips/plant and 0.47 to 4.0 leafhoppers/plant, respectively. The module T5 was significantly superior to Farmer Practice during both Kharif and Rabi-summer seasons.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"24 1","pages":"592-597"},"PeriodicalIF":0.8,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82553774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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