Pub Date : 2020-03-21DOI: 10.22037/AMLS.V4I4.28130
S. Shahbazi
Background: Menorrhagia is the most common type of abnormal uterine bleeding, caused by disruption of hormonal regulation, uterine function or blood clotting. Developing an effective diagnostic strategy will improve patient's quality of life and management. Here we investigated the links between hematological characteristics and prolonged menstruation to estimate the importance of the first line coagulation screening tests in young women . Materials and Methods: In a case-control design and under supervision of a specialist, 43 cases and 104 age matched controls were selected. Menstrual characteristics were evaluated by a standardized questionnaire distributed to high school and university young students. Hematological indices including first line coagulation screening tests were performed for both groups and statistically assessed. Result: Statistical analysis showed that prolonged menstrual bleeding is significantly correlated to prolonged bleeding time ( p value , 0.01) as well as red blood cell count ( p value , 0.04). The O blood group showed the greatest contribution to the bleeding periods longer than 7 days (53.4 %(. Additional coagulation tests revealed one coagulation factor VII deficiency patient. Conclusion: The results of the present study revealed the importance of menorrhagia management in young women and showed a significant correlation between prolonged menstrual bleeding and blood types . Our study findings also suggest a significant association between prolonged menstruation and bleeding time test, emphasizing on the role of blood coagulation traits in susceptibility to heavy menstrual bleeding.
{"title":"Hematological indices in prolonged menstruation: New roles for blood groups and coagulation factors","authors":"S. Shahbazi","doi":"10.22037/AMLS.V4I4.28130","DOIUrl":"https://doi.org/10.22037/AMLS.V4I4.28130","url":null,"abstract":"Background: Menorrhagia is the most common type of abnormal uterine bleeding, caused by disruption of hormonal regulation, uterine function or blood clotting. Developing an effective diagnostic strategy will improve patient's quality of life and management. Here we investigated the links between hematological characteristics and prolonged menstruation to estimate the importance of the first line coagulation screening tests in young women . Materials and Methods: In a case-control design and under supervision of a specialist, 43 cases and 104 age matched controls were selected. Menstrual characteristics were evaluated by a standardized questionnaire distributed to high school and university young students. Hematological indices including first line coagulation screening tests were performed for both groups and statistically assessed. Result: Statistical analysis showed that prolonged menstrual bleeding is significantly correlated to prolonged bleeding time ( p value , 0.01) as well as red blood cell count ( p value , 0.04). The O blood group showed the greatest contribution to the bleeding periods longer than 7 days (53.4 %(. Additional coagulation tests revealed one coagulation factor VII deficiency patient. Conclusion: The results of the present study revealed the importance of menorrhagia management in young women and showed a significant correlation between prolonged menstrual bleeding and blood types . Our study findings also suggest a significant association between prolonged menstruation and bleeding time test, emphasizing on the role of blood coagulation traits in susceptibility to heavy menstrual bleeding.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87879574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-21DOI: 10.22037/AMLS.V4I4.24922
F. H. Shandiz, S. Aledavood, Rozita Delghandi, M. Fani, Aida Gholoobi, Seyed Muhammad Yahyazadeh Mashhadi, Mohsen Abdoli, Hamed Gouklani, Sina Geraily, Z. Meshkat
Background: Hepatocellular carcinoma (HCC), the most common type of primary liver cancer, is a life-threatening disease worldwide. The aim of this study was to investigate the epidemiology and clinical features of HCC patients who referred to Omid hospital in Mashhad, northeast of Iran. Materials and Methods: In this cross sectional retrospective study, we reviewed the medical records of patients who referred to Omid hospital – a cancer research center– in Mashhad during 1991 to 2012. Medical records of 29 patients with primary liver cancer proven with biopsy, CT scan or MRI were analyzed in this study. Results: Of 25 eligible cases, 68% were men and the rest were women. The majority of HCC patients were in the 60-69 age group. Also, 44% of patients were found to be hepatitis B virus surface antigen (HBsAg) positive. Conclusion: The age distribution and male preponderance of HCC patients observed in the present study in line with other conducted studies in Iran and other countries. Since this is a retrospective study, a comprehensive study with a larger sample size in a case-control study is needed to establish other HCC-related factors in our province.
{"title":"Epidemiology and Clinical Characteristics of Patients with Hepatocellular Carcinoma in North-East of Iran","authors":"F. H. Shandiz, S. Aledavood, Rozita Delghandi, M. Fani, Aida Gholoobi, Seyed Muhammad Yahyazadeh Mashhadi, Mohsen Abdoli, Hamed Gouklani, Sina Geraily, Z. Meshkat","doi":"10.22037/AMLS.V4I4.24922","DOIUrl":"https://doi.org/10.22037/AMLS.V4I4.24922","url":null,"abstract":"Background: Hepatocellular carcinoma (HCC), the most common type of primary liver cancer, is a life-threatening disease worldwide. The aim of this study was to investigate the epidemiology and clinical features of HCC patients who referred to Omid hospital in Mashhad, northeast of Iran. Materials and Methods: In this cross sectional retrospective study, we reviewed the medical records of patients who referred to Omid hospital – a cancer research center– in Mashhad during 1991 to 2012. Medical records of 29 patients with primary liver cancer proven with biopsy, CT scan or MRI were analyzed in this study. Results: Of 25 eligible cases, 68% were men and the rest were women. The majority of HCC patients were in the 60-69 age group. Also, 44% of patients were found to be hepatitis B virus surface antigen (HBsAg) positive. Conclusion: The age distribution and male preponderance of HCC patients observed in the present study in line with other conducted studies in Iran and other countries. Since this is a retrospective study, a comprehensive study with a larger sample size in a case-control study is needed to establish other HCC-related factors in our province.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89672664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-20DOI: 10.22037/AMLS.V4I3.25579
Maryam Farahmand Sadr, Nima Fadakar, Susan Mansuri Mehrabadi, Yasaman Zolghadrasli, M. Poursadeghfard
Background: Cerebral sinus venous thrombosis (CSVT) is a fatal condition and should be considered in all patients with acute new onset headache. D-dimer has been shown to be a sensitive diagnostic tool in deep vein thrombosis and pulmonary thromboembolism. The purpose of this study was to investigate whether this test could be useful in the diagnosis of CSVT. Methods : In this prospective study, we reviewed patients referring to Nemazee hospital with presentations suggestive of CSVT. Diagnosis was established, using MRI and MRV. Serum D-dimer level was checked among 24 hours after hospital admission. Results: From 117 enrolled patients, 37 (31.6%) patients had CSVT. The reported D-dimer levels showed negative (< 500 ng/ml) in 21 (56.76%) and positive level in 16 (43.24 %) patients who had CSVT. Also, D-dimer was negative in 66 (82.5%) and positive in 14 (17.5%) patients who did not have CSVT. These results demonstrated a sensitivity of 43.24%, specificity of 82.5%, positive predictive value of 53.3%, and negative predictive value of 75.86%. Conclusion: Normal D-dimer levels make the presence of CSVT very unlikely; our study suggests that D-dimer test may guide us to approach patients who are suspected with CSVT in emergency situations with limited access to MRI/MRV. However, every patient with D-dimer level more than 500 ng/mL and high clinical suspicion of CSVT should undergo MRI/MRV. In addition, our results showed a negative D-dimer test was not a reliable assay to completely rule out CSVT. However, we recommend further studies to confirm our results.
{"title":"The role of D-dimer in diagnosis of cerebral sinus venous thrombosis","authors":"Maryam Farahmand Sadr, Nima Fadakar, Susan Mansuri Mehrabadi, Yasaman Zolghadrasli, M. Poursadeghfard","doi":"10.22037/AMLS.V4I3.25579","DOIUrl":"https://doi.org/10.22037/AMLS.V4I3.25579","url":null,"abstract":"Background: Cerebral sinus venous thrombosis (CSVT) is a fatal condition and should be considered in all patients with acute new onset headache. D-dimer has been shown to be a sensitive diagnostic tool in deep vein thrombosis and pulmonary thromboembolism. The purpose of this study was to investigate whether this test could be useful in the diagnosis of CSVT. Methods : In this prospective study, we reviewed patients referring to Nemazee hospital with presentations suggestive of CSVT. Diagnosis was established, using MRI and MRV. Serum D-dimer level was checked among 24 hours after hospital admission. Results: From 117 enrolled patients, 37 (31.6%) patients had CSVT. The reported D-dimer levels showed negative (< 500 ng/ml) in 21 (56.76%) and positive level in 16 (43.24 %) patients who had CSVT. Also, D-dimer was negative in 66 (82.5%) and positive in 14 (17.5%) patients who did not have CSVT. These results demonstrated a sensitivity of 43.24%, specificity of 82.5%, positive predictive value of 53.3%, and negative predictive value of 75.86%. Conclusion: Normal D-dimer levels make the presence of CSVT very unlikely; our study suggests that D-dimer test may guide us to approach patients who are suspected with CSVT in emergency situations with limited access to MRI/MRV. However, every patient with D-dimer level more than 500 ng/mL and high clinical suspicion of CSVT should undergo MRI/MRV. In addition, our results showed a negative D-dimer test was not a reliable assay to completely rule out CSVT. However, we recommend further studies to confirm our results.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74837256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-18DOI: 10.22037/AMLS.V4I3.25392
M. Rezvani
Background: Acute lymphoblastic leukemia (ALL) is one of the major lymphoid malignancies and the most common hematologic malignancy in children. ALL is characterized by the presence of malignant lymphoblasts in the blood so that immature lymphocytes cannot become mature and thus do not have an adult cell function. Although it is not unusual in adults, it usually affects children. Most children with this disease are recovered from therapeutic protocols. But the relapse is common after recovery or during the treatment. Various factors are supposed to contribute to the relapse of the disease. One of these factors that is likely to be effective in the recurrence of ALL is the FAIM3 protein (an FCuR), or the Fas inhibitory molecule-3 (FAIM3). The aim of this study was to investigate FAIM3 (TOSO) as a new prognostic factor in ALL. Materials and methods: In this study, 19 patients with newly diagnosed and 17 patients with relapsed ALL were included. FAIM3 gene expression was measured with the qRT-PCR method. Results: The expression level of FAIM3 in relapsed patients was 5.44 folds higher than newly diagnosed ALL patients. Conclusion: Prognosis of ALL is usually well-proven in children and can be cured. However, recurrence of the disease is common. At the molecular level, there are several factors that are referred to as the "factor involved in the relapse" of the disease. These factors increase the survival of the leukemic cells. According to the results of the present study, gene expression level of FAIM3 as an anti-apoptotic factor has increased in relapsed ALL lymphoblasts, compared with new diagnosed patients. Therefore, FAIM3 can be considered as a contributing factor in the relapse of the disease.
{"title":"Comparison of FAIM3 gene expression between new cases of ALL and relapsed ALL","authors":"M. Rezvani","doi":"10.22037/AMLS.V4I3.25392","DOIUrl":"https://doi.org/10.22037/AMLS.V4I3.25392","url":null,"abstract":"Background: Acute lymphoblastic leukemia (ALL) is one of the major lymphoid malignancies and the most common hematologic malignancy in children. ALL is characterized by the presence of malignant lymphoblasts in the blood so that immature lymphocytes cannot become mature and thus do not have an adult cell function. Although it is not unusual in adults, it usually affects children. Most children with this disease are recovered from therapeutic protocols. But the relapse is common after recovery or during the treatment. Various factors are supposed to contribute to the relapse of the disease. One of these factors that is likely to be effective in the recurrence of ALL is the FAIM3 protein (an FCuR), or the Fas inhibitory molecule-3 (FAIM3). The aim of this study was to investigate FAIM3 (TOSO) as a new prognostic factor in ALL. Materials and methods: In this study, 19 patients with newly diagnosed and 17 patients with relapsed ALL were included. FAIM3 gene expression was measured with the qRT-PCR method. Results: The expression level of FAIM3 in relapsed patients was 5.44 folds higher than newly diagnosed ALL patients. Conclusion: Prognosis of ALL is usually well-proven in children and can be cured. However, recurrence of the disease is common. At the molecular level, there are several factors that are referred to as the \"factor involved in the relapse\" of the disease. These factors increase the survival of the leukemic cells. According to the results of the present study, gene expression level of FAIM3 as an anti-apoptotic factor has increased in relapsed ALL lymphoblasts, compared with new diagnosed patients. Therefore, FAIM3 can be considered as a contributing factor in the relapse of the disease.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"109 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91462445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-17DOI: 10.22037/AMLS.V4I4.25635
Reza Aramideh Khouy, M. H. Tehrani, H. Nosrati, Maryam Esghaei
Various factors are involved in the incidence of some diseases like autoimmune, psychiatric and cancerous ones. One of these probable factors is considered as the endogenous retroviruses, for example, proviruses that have been introduced in previous generations in some organisms' genome, and make up over 8% of the human genome. Recent studies have indicated that these factors and their related products (including RNA, cytosolic DNA, and proteins) may affect and also change the host cell function and immune system. This review summarizes the detailed information about the structure, classification, and pathogenesis mechanism of human endogenous retroviruses and their relationship with the autoimmune diseases and some kinds of cancers.
{"title":"A Review on Relationship Between Human Endogenous Retrovirus Groups and Human Diseases","authors":"Reza Aramideh Khouy, M. H. Tehrani, H. Nosrati, Maryam Esghaei","doi":"10.22037/AMLS.V4I4.25635","DOIUrl":"https://doi.org/10.22037/AMLS.V4I4.25635","url":null,"abstract":"Various factors are involved in the incidence of some diseases like autoimmune, psychiatric and cancerous ones. One of these probable factors is considered as the endogenous retroviruses, for example, proviruses that have been introduced in previous generations in some organisms' genome, and make up over 8% of the human genome. Recent studies have indicated that these factors and their related products (including RNA, cytosolic DNA, and proteins) may affect and also change the host cell function and immune system. This review summarizes the detailed information about the structure, classification, and pathogenesis mechanism of human endogenous retroviruses and their relationship with the autoimmune diseases and some kinds of cancers.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"162 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90145589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-08DOI: 10.22037/AMLS.V4I3.26269
R. Ebrahimi, Alireza Bahiraee, Naghmeh Jannat Alipour, K. Toolabi, S. Emamgholipour
Background: Several studies suggested that beta-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and 18S rRNA are expressed constitutively and contribute to the fundamental reference actions essential for cell viability and maintenance. However, there are inconsistency in this regard. Hence, we aimed to evaluate the accuracy of these three potential reference genes for Real‐Time quantitative reverse transcriptase‐polymerase chain reaction (qRT‐PCR) application for normalization in two types of human adipose tissues. Materials and Methods: Subcutaneous and visceral fat tissues were derived from 19 healthy and 20 obese subjects and RT-qPCR was applied to determine the expression levels of beta-actin, GAPDH, and18S rRNA. Results: The gene expression level of beta-actin, GAPDH, and 18S rRNA was essentially the same in the subcutaneous and visceral fat tissues of all participants (P>0.05). Hence, all considered housekeeping genes displayed high expression stability and the analysis revealed that normalization to all of these three housekeeping genes gave a result that satisfactorily reflected the acceptable mRNA expression levels in adipose tissues. Conclusion: Collectively, our findings suggest of beta-actin, GAPDH, and18S rRNA as reference genes applicable in human adipose tissue in the context of obesity.
{"title":"Evaluation of the Housekeeping Genes; β‐Actin, Glyceraldehyde‐3‐Phosphate‐Dehydrogenase, and 18S rRNA for Normalization in Real‐Time Polymerase Chain Reaction Analysis of Gene Expression in Human Adipose Tissue","authors":"R. Ebrahimi, Alireza Bahiraee, Naghmeh Jannat Alipour, K. Toolabi, S. Emamgholipour","doi":"10.22037/AMLS.V4I3.26269","DOIUrl":"https://doi.org/10.22037/AMLS.V4I3.26269","url":null,"abstract":"Background: Several studies suggested that beta-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and 18S rRNA are expressed constitutively and contribute to the fundamental reference actions essential for cell viability and maintenance. However, there are inconsistency in this regard. Hence, we aimed to evaluate the accuracy of these three potential reference genes for Real‐Time quantitative reverse transcriptase‐polymerase chain reaction (qRT‐PCR) application for normalization in two types of human adipose tissues. Materials and Methods: Subcutaneous and visceral fat tissues were derived from 19 healthy and 20 obese subjects and RT-qPCR was applied to determine the expression levels of beta-actin, GAPDH, and18S rRNA. Results: The gene expression level of beta-actin, GAPDH, and 18S rRNA was essentially the same in the subcutaneous and visceral fat tissues of all participants (P>0.05). Hence, all considered housekeeping genes displayed high expression stability and the analysis revealed that normalization to all of these three housekeeping genes gave a result that satisfactorily reflected the acceptable mRNA expression levels in adipose tissues. Conclusion: Collectively, our findings suggest of beta-actin, GAPDH, and18S rRNA as reference genes applicable in human adipose tissue in the context of obesity.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79693376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Esmailidehaj, S. Mirhosseini, Zakieh Sadat Sheikhalishahi
Background and Aim: Atrial fibrillation is the most common arrhythmia after coronary artery bypass grafting (CABG) and occurs in up to 30% of patients with heart failure. Mesenchymal stem cells (MSCs) can regenerate and improve cardiac function after tissue damage and are used in clinical trials. Due to the possible ability of MSCs to improve cardiac functions, in this work, we aimed to examine the probable association of the MSCs proliferation rate with the requirement for antiarrhythmic drugs in patients with severe left ventricular dysfunction after off-pump CABG. Methods: Bone marrow samples were obtained from the sternum of 30 patients who underwent off-pump CABG at Afshar Hospital and Seyed Al-Shohada Hospital. For calculating MSCs doubling time, the cells were counted after 4, 7, and 14 days using trypan-blue color and a hemocytometer. Results: There were no significant differences between MSCs' doubling time and the patient's age and gender. The percentage of women patients who require antiarrhythmic medicine was significantly higher than men after surgery. Also, we demonstrated that the BMSCs doubling time in female patients who received antiarrhythmic drugs was less than that of male patients who received antiarrhythmic drugs, but these differences were not significant. Conclusion: Based on this research, we concluded that women patients who received antiarrhythmic drugs were significantly higher than men, but there was no apparent relevancy between MSCs doubling time and antiarrhythmic drugs requirement in patients with severe left ventricular dysfunction. *Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: z.sheikhalishahi@gmail.com Please cite this article as: Esmailidehaj M, Mirhosseini SJ, Sheikhalishahi ZS. Association between Bone Marrow Mesenchymal Stem Cell Characterizations and the Administration of Antiarrhythmic Drugs in Patients with Severe Left Ventricular Dysfunction after Off-Pump Bypass Surgery. Arch Med Lab Sci. 2020;6:e2. https://doi.org/10.22037/amls.v6.31297
{"title":"Association between Bone Marrow Mesenchymal Stem Cell Characterizations and the Administration of Antiarrhythmic Drugs in Patients with Severe Left Ventricular Dysfunction after Off-Pump Bypass Surgery","authors":"M. Esmailidehaj, S. Mirhosseini, Zakieh Sadat Sheikhalishahi","doi":"10.22037/AMLS.V6.31297","DOIUrl":"https://doi.org/10.22037/AMLS.V6.31297","url":null,"abstract":"Background and Aim: Atrial fibrillation is the most common arrhythmia after coronary artery bypass grafting (CABG) and occurs in up to 30% of patients with heart failure. Mesenchymal stem cells (MSCs) can regenerate and improve cardiac function after tissue damage and are used in clinical trials. Due to the possible ability of MSCs to improve cardiac functions, in this work, we aimed to examine the probable association of the MSCs proliferation rate with the requirement for antiarrhythmic drugs in patients with severe left ventricular dysfunction after off-pump CABG. \u0000Methods: Bone marrow samples were obtained from the sternum of 30 patients who underwent off-pump CABG at Afshar Hospital and Seyed Al-Shohada Hospital. For calculating MSCs doubling time, the cells were counted after 4, 7, and 14 days using trypan-blue color and a hemocytometer. \u0000Results: There were no significant differences between MSCs' doubling time and the patient's age and gender. The percentage of women patients who require antiarrhythmic medicine was significantly higher than men after surgery. Also, we demonstrated that the BMSCs doubling time in female patients who received antiarrhythmic drugs was less than that of male patients who received antiarrhythmic drugs, but these differences were not significant. \u0000Conclusion: Based on this research, we concluded that women patients who received antiarrhythmic drugs were significantly higher than men, but there was no apparent relevancy between MSCs doubling time and antiarrhythmic drugs requirement in patients with severe left ventricular dysfunction. \u0000*Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: z.sheikhalishahi@gmail.com \u0000Please cite this article as: Esmailidehaj M, Mirhosseini SJ, Sheikhalishahi ZS. Association between Bone Marrow Mesenchymal Stem Cell Characterizations and the Administration of Antiarrhythmic Drugs in Patients with Severe Left Ventricular Dysfunction after Off-Pump Bypass Surgery. Arch Med Lab Sci. 2020;6:e2. https://doi.org/10.22037/amls.v6.31297","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78859886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Aim: Regular exercise training is the principal non-pharmacological method for the prevention and treatment of metabolic syndrome or other obesity-related diseases. We investigated the effects of aerobic training on leptin and cardiovascular risk factors in middle-aged men with metabolic syndrome. Methods: Twenty-six sedentary middle-aged males with metabolic syndrome aged 40 ± 5 years were randomly assigned to two groups as exercise (aerobic training, n =13) and control (no training, n =13). The exercise programs were performed 3 days a week for 10 weeks at 55-75% of HRmax. Fasting blood samples were taken before and after the training period for measuring serum leptin and triglyceride (TG), total cholesterol (TC), LDL, and HDL as cardiovascular risk factors. Results: No significant differences were observed between groups concerning anthropometric and clinical markers at baseline (p>0.05). Aerobic intervention resulted in significant decrease in anthropometric markers (abdominal circumference, body mass index, body fat percentage; p < 0.05), serum leptin (p = 0.026), TG (p = 0.001) and HDL (p = 0.032) in exercise group, but significant changes were not found in TC (p = 0.522) and LDL (p = 0.546). There were no changes in all measured variables in the control group. Conclusion: Based on our finding, it seems that regular aerobic exercise is associated with improved serum leptin and cardiovascular function in patients with metabolic syndrome. *Corresponding Author: Mojtaba Eizadi; Email: izadimojtaba2006@yahoo.com Please cite this article as: Naseri Rad R, Eizadi M. Regular Exercise Training as a Principal Non-Pharmacological Method Affects Serum Leptin and Cardiovascular Risk Factors in Men with Metabolic Syndrome. Arch Med Lab Sci. 2020;6:1-8 (e1). https://doi.org/10.22037/amls.v6.31643
{"title":"Regular Exercise Training as a Principal Non-Pharmacological Method Affects Serum Leptin and Cardiovascular Risk Factors in Men with Metabolic Syndrome","authors":"Reza Naseri Rad, M. Eizadi","doi":"10.22037/AMLS.V6.31643","DOIUrl":"https://doi.org/10.22037/AMLS.V6.31643","url":null,"abstract":"Background and Aim: Regular exercise training is the principal non-pharmacological method for the prevention and treatment of metabolic syndrome or other obesity-related diseases. We investigated the effects of aerobic training on leptin and cardiovascular risk factors in middle-aged men with metabolic syndrome. \u0000Methods: Twenty-six sedentary middle-aged males with metabolic syndrome aged 40 ± 5 years were randomly assigned to two groups as exercise (aerobic training, n =13) and control (no training, n =13). The exercise programs were performed 3 days a week for 10 weeks at 55-75% of HRmax. Fasting blood samples were taken before and after the training period for measuring serum leptin and triglyceride (TG), total cholesterol (TC), LDL, and HDL as cardiovascular risk factors. \u0000Results: No significant differences were observed between groups concerning anthropometric and clinical markers at baseline (p>0.05). Aerobic intervention resulted in significant decrease in anthropometric markers (abdominal circumference, body mass index, body fat percentage; p < 0.05), serum leptin (p = 0.026), TG (p = 0.001) and HDL (p = 0.032) in exercise group, but significant changes were not found in TC (p = 0.522) and LDL (p = 0.546). There were no changes in all measured variables in the control group. \u0000Conclusion: Based on our finding, it seems that regular aerobic exercise is associated with improved serum leptin and cardiovascular function in patients with metabolic syndrome. \u0000*Corresponding Author: Mojtaba Eizadi; Email: izadimojtaba2006@yahoo.com \u0000Please cite this article as: Naseri Rad R, Eizadi M. Regular Exercise Training as a Principal Non-Pharmacological Method Affects Serum Leptin and Cardiovascular Risk Factors in Men with Metabolic Syndrome. Arch Med Lab Sci. 2020;6:1-8 (e1). https://doi.org/10.22037/amls.v6.31643","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75032038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-14DOI: 10.22037/AMLS.V4I1.20631
S. Athari
The respiratory system is exposed to the potentially harmful environment agents. More importantly, respiratory system infection is an important risk factor for inflammation and some pathogens can be main responsible of asthma. Phagocytosis is a main mechanism to eliminate of microbial infection. Phagocytic clearance may control asthma pathogenesis. In asthma, cytokines balance may be changed, therefore we investigated possible change in phagocytes in the present study.14 male Balb/c mice were divided into two control and asthmatic group. Asthma model in mice was produced by ovalbumin. Peripheral blood mononuclear cells were separated and reduction nitro blue tetrazolium and latex bead florescence phagocytosis tests were done. There was no significant difference in phagocytosis and NBT reduction test between asthmatic and control groups (P≤0.05).Airway inflammation and unbalancing of cytokines in asthma might modulate phagocytosis function. Therefore, asthmatic patient might be more susceptible to airway infection but there was not any notable changes in phagocytosis.
{"title":"Investigation of Peripheral Blood Mononuclear Cells Phagocytosis in Allergic Asthma Mice Model","authors":"S. Athari","doi":"10.22037/AMLS.V4I1.20631","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.20631","url":null,"abstract":"The respiratory system is exposed to the potentially harmful environment agents. More importantly, respiratory system infection is an important risk factor for inflammation and some pathogens can be main responsible of asthma. Phagocytosis is a main mechanism to eliminate of microbial infection. Phagocytic clearance may control asthma pathogenesis. In asthma, cytokines balance may be changed, therefore we investigated possible change in phagocytes in the present study.14 male Balb/c mice were divided into two control and asthmatic group. Asthma model in mice was produced by ovalbumin. Peripheral blood mononuclear cells were separated and reduction nitro blue tetrazolium and latex bead florescence phagocytosis tests were done. There was no significant difference in phagocytosis and NBT reduction test between asthmatic and control groups (P≤0.05).Airway inflammation and unbalancing of cytokines in asthma might modulate phagocytosis function. Therefore, asthmatic patient might be more susceptible to airway infection but there was not any notable changes in phagocytosis.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76928445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-11DOI: 10.22037/AMLS.V4I2.25568
S. Athari
Background: The present study is on the development of a data mining algorithm for finding the influential factors on the hospitalization of patients subject to chronic obstructive pulmonary disease. Materials and Methods: This is a descriptive analytical study conducted cross sectionally in 2017 on a research community of 150 people with disease symptoms referred to clinics and hospitals across Tehran (Iran). The people were surveyed by a self-designed questionnaire, including queries on life style and family information. The sampling was simple intuitive from previously published studies. The modeling of the data was based on the CRISP method. The C5 decision tree algorithm was used and the data was analyzed by RapidMiner software. Results: The common symptoms of the patients were found to be shortness of breath, cough, chest pain, sputum, continuous cold, and cyanogens. Besides, the family history, smoking, and exposure to allergic agents were other influential factors on the disease. After accomplishment of this study, the results were consulted with the experts of the field. Conclution: It is concluded that data mining can be applied for excavation of knowledge from the gathered data and for determination of the effective factors on patient conditions. Accordingly, this model can successfully predict the disease status of any patient from its symptoms.
{"title":"A data mining algorithm for determination of influential factors on the hospitalization of patients subject to chronic obstructive pulmonary disease","authors":"S. Athari","doi":"10.22037/AMLS.V4I2.25568","DOIUrl":"https://doi.org/10.22037/AMLS.V4I2.25568","url":null,"abstract":"Background: The present study is on the development of a data mining algorithm for finding the influential factors on the hospitalization of patients subject to chronic obstructive pulmonary disease. Materials and Methods: This is a descriptive analytical study conducted cross sectionally in 2017 on a research community of 150 people with disease symptoms referred to clinics and hospitals across Tehran (Iran). The people were surveyed by a self-designed questionnaire, including queries on life style and family information. The sampling was simple intuitive from previously published studies. The modeling of the data was based on the CRISP method. The C5 decision tree algorithm was used and the data was analyzed by RapidMiner software. Results: The common symptoms of the patients were found to be shortness of breath, cough, chest pain, sputum, continuous cold, and cyanogens. Besides, the family history, smoking, and exposure to allergic agents were other influential factors on the disease. After accomplishment of this study, the results were consulted with the experts of the field. Conclution: It is concluded that data mining can be applied for excavation of knowledge from the gathered data and for determination of the effective factors on patient conditions. Accordingly, this model can successfully predict the disease status of any patient from its symptoms.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90464313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：