Medical laboratory sciences最新文献

{"title":"Effects of Biochanin A on Resistin, Adiponectin and some stress oxidative markers in normal and STZ- Induced Diabetic Rats","authors":"Z. Salemi, H. Ghasemi, Ali Morovati, H. Sadri","doi":"10.22037/AMLS.V4I2.24908","DOIUrl":"https://doi.org/10.22037/AMLS.V4I2.24908","url":null,"abstract":"Background : Elevated serum level of adiponectin and insulin and decreased serum resistin can improve glucose metabolism. Biochanin A (BCA) is a flavonoid of Soybean that shows antioxidant properties. This study was aimed to examine the effect of BCA on FBS, oxidative stress and serum levels of adiponectin, resistin and insulin in rats with type 1 diabetes. Material and method: The rats were randomly divided into five groups (n=6). BCA was administered orally in doses of 10 and 15 mg/kg of body weight. Insulin, resistin and adiponectin were measured using ELISA kits. The activity of Gamma-Glutamyltransferase (GGT), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and the levels of Glutathione (GSH) were examined. Results : The results showed that BCA treatment significantly reduced the FBS level in diabetic rats (p<0.05). Serum insulin was significantly increased in the BCA treated diabetic rats (p<0.05). Moreover, GGT activity and GSH was significantly increased in treated rats (p<0.05). Our findings revealed that the administration of BCA significantly increased the serum adiponectin (p<0.05). Additionally, serum resistin levels were remarkably decreased in treated rats (p<0.05). Conclusion: Taken together, BCA represents a natural phytoestrogen that has an important role in improvement of glucose metabolism by regulating of adipokines secretion; also our findings revealed the beneficial effects of BCA against oxidative stress in diabetes","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85541715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What is the best laboratory method for diagnosis of Herpes Simplex Virus in genital infections","authors":"Hamed Sadegh Mazji, Z. Meshkat, M. Rezayi, S. Rezaee, E. Aryan, Hamed Gouklani, M. Fani, A. Jalili","doi":"10.22037/AMLS.V4I3.24924","DOIUrl":"https://doi.org/10.22037/AMLS.V4I3.24924","url":null,"abstract":"Genital infection caused by Herpes simplex virus (HSV) is one of the most common health problems, worldwide. Several methods such as cell culture, serological and molecular methods have been used to detect this virus. Currently, Real-Time Polymerase Chain Reaction (Real time-PCR) technique is widely used due to its high sensitivity and specificity. Besides, Real time-PCR can be employed in the follow-up of therapeutic effects in HSV-infected person who is being treated with antiretroviral drugs. We conducted a review on traditional and current diagnostic methods with a focus on their limitations in the diagnosis of HSV infection.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87061236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of different dietary levels of Pennyroyal (Mentha Pulegium L.), probiotic and antibiotic on performance, carcass characteristics and, selected nutrients digestibility in broiler chickens","authors":"A. Hassanabadi, A. Abedini, N. Afzali, H. Kermanshahi","doi":"10.22037/AMLS.V3I2.17572","DOIUrl":"https://doi.org/10.22037/AMLS.V3I2.17572","url":null,"abstract":"Background: The aim of this study was to investigate the effects of different dietary levels of Pennyroyal ( Mentha Pulegium L.) essential oil (PEO), probiotic (Bioplus 2B) and antibiotic (Flavophospholipol) on performance, carcass characteristics and nutrients digestibility in broiler chickens in a completely randomize design (CRD). Material and methods : The treatments included: A corn-wheat-soybean meal basal diet without any additives as control group and adding three levels of Flavophospholipol (0.015, 0.03 and 0.05 % of diet), three levels of BioPlus-B2 (0.1, 0.2 and 0.3 % of diet) and three levels of Mentha pulegium essential oil (0.03, 0.05 and 0.07 % of diet) to the basal diet. 5 replicates of 12 chicks were allocated to each experimental treatment. Results : The results showed that the treatments significantly affected body weight gain (WG) and feed conversion ratio (FCR) in the all experimental periods (P 0.05). PEO at the level of 0.07% significantly decreased WG during 11-25 d (P 0.05). Breast and abdominal fat percentage were not significantly affected by treatments (P>0.05). Lowest crude protein (CP) digestibility was observed in 0.015% antibiotic treatment that was significantly lower than 0.1% probiotic treatment (P<0.05). Conclusion : More significantly, dietary supplements represented desirable performance in compared to antibiotics and control group. Hence, the possible usage of these components as antibiotics alternatives in poultry feeds should be outlined in future","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90922213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural killer cell biology and its effect on graft versus host disease","authors":"Mahshid AkhavanRahnama, M. Soleimani, Mina Soufi, N. Moradi","doi":"10.22037/AMLS.V3I4.20365","DOIUrl":"https://doi.org/10.22037/AMLS.V3I4.20365","url":null,"abstract":"Natural killer (NK) cells were originally described in terms of their function. NK cells are of lymphoid origin and are found in the peripheral blood, spleen, and bone marrow, as well as other tissues. These cells are large, radio-resistant and granular lymphocytes that represent an important arm of innate immunity and are thought to play a critical role in the immune surveillance against tumors and virally infected cells. Allogeneic bone marrow transplantation (BMT) has proven to be an effective treatment for hematologic malignancies and some solid tumors. One of the major challenges of allo-stem cell transplantation (SCT) is to reduce the incidence and severity of GVHD while boosting the graft-versus-leukemia (GVL) effect. In the setting of allo-SCT, the reconstitution of NK cells is of notable interest due to their known capability to induce GVL without GVHD. Clinical applications of NK cells have been inspired by recognition of their potent anticancer activity. These studies discussed a solid basis for development of future NK cell trials for cancer therapy by minimizing risks and toxicities.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90689008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An investigation into the prevalence of diabetes in the Northwest of Iran: The case study of Maragheh City","authors":"Shiva Asadianfam, N. Abdollahi","doi":"10.22037/AMLS.V3I1.16165","DOIUrl":"https://doi.org/10.22037/AMLS.V3I1.16165","url":null,"abstract":"Background : Diabetes is a type of metabolic diseases whose common characteristic is the increase in blood sugar. The prevalence of diabetes worldwide has progressively reached epidemic proportions. Its complications are microvascular and macrovascular complications, gastrointestinal and genitourinary disorders, infection and glaucoma. This study was designed to examine the incidence of diabetes in case of gender and age differences, and to investigate its complications .to better understand related etiology. Materials and Methods : This research was a cross-sectional study undertaken on 380 diabetic patients referred to Amir-al-Momenin Hospital in Maragheh. The data were collected using patient records and analysed using SPSS software. The frequency and frequency percent of the qualitative data and the mean of the quantitative data were reported. Results : The analysis suggested that among 380 diabetic patients, 248 were female and 132 were male, and 95.3% of those surveyed were married. The mean age of the patients under investigation was 57.66. 303 patients (79.7%) of the participants had a history of drug use, and the drugs are mainly cardiovascular drugs (35.8%), metformin (33.9%), and glibenclamide (28.2%). Over 50% of the participants had a history of illness, particularly cardiovascular disease (48.2%), while the least number of the participant had seizures. The highest and lowest number of patients had the blood sugar range of 424-325 (84 patients, 22.1%) and the blood sugar range of 622-523 (17 patients, 4.5%) , respectively. Moreover, the largest number of subjects (65 patients, 17.1%) had the blood cholesterol in the range of 325-271. About 75 participants had too much LDL. Besides, no statistically significant relationship was found between diabetes and white blood cells . 172 subjects developed complications. 70 patients, 68 patients, 55 patients, 51 patients, 14 patients, 13 patients, and 5 patients had renal, gastrointestinal, cutaneous, respiratory, cardiovascular, ocular complications, and amputations, respectively. About 110 subjects had various habits such as special diet (n = 44), smoking (n = 39), addiction (n=26) and drinking (n=3). Among 11 subjects of gestational diabetes mellitus (GDM), most had a high body mass index (BMI), the protein in the urine of about 18 percent of the subjects was positive, and regarding glucose disposal  in the urine, 53 subjects were +, 64 subjects were +, 42 subjects were +++, 1 subject was ++++. Seventy participants had ketone disposal, which indicates kidney disorder. Conclusion : The results revealed a higher prevalence of disease among middle-aged and older people in the society. The mean of B.M.I is 27.28 showing overweight in our population. The results also indicated a higher prevalence of hyperglycemia compared to hypoglycemia that may be due to very close relationship between diabetes and cardiovascular disorders, high levels of blood sugar in most of the diabetic patients. In additio","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73994314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the ratio of cross-matched to transfused blood amounts in pregnant patients in Alzahra Hospital in Tabriz before and after the implementation of the health reform program in 2013-2015","authors":"Behzad Bahramzadeh, Monireh Maleki, N. Asbaghi, M. Javani, Samaneh Tajfar, M. Shams","doi":"10.22037/AMLS.V2I4.17182","DOIUrl":"https://doi.org/10.22037/AMLS.V2I4.17182","url":null,"abstract":"Background: Postpartum hemorrhage is the most common cause of mortality in women with vaginal or cesarean delivery. WHO statistics shows that about 500 thousands women have died of complications related to pregnancy or during childbirth in 2013. haemorrhage probability is the main reason to order blood requests in delivery and cesarean units. The purpose of this study is to evaluate the ratio of cross-matched to transfused blood in pregnant women during one year before and after the implementation of health reform program. Materials and Methods: In this retrospective descriptive study, the requests of blood reserves for pregnant patients with the gravid of 34±7 weeks and the age of 30±16 years old in two periods, before and after the implementation of health reform program were being collected and compared. Blood group antiserums and anti-human globulins with bovine albumins were purchased from LORN company and Baharafshan Company respectively. Results: The total number of requests for reserved cross-matched blood during the year before the health reform program were 2837 units which 277 of them had been transfused and a year later the total number of requests was for 3029 units, which 270 of them had been transfused. This study showed 6.76 percent increase in requests and 2.53 percent decrease in transfusion in comparison with its past year. Conclusion: The implementation of health reform program have accompanied by relative reduction of cesarean but considerable increase in vaginal delivery, and to prevent blood loss complications, the requests for reserved cross-matched blood have been increased without noticeable change in the proportion of necessarily transfused amount of them.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75793396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association of serum level of CTRP1 with Common Bile Duct Diameter and Other Manifestations in Patients with Non-alcoholic Fatty Liver","authors":"R. Ebrahimi, Shahabedin Zand, Mehrnoosh Shanaki","doi":"10.22037/AMLS.V4I1.26273","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.26273","url":null,"abstract":"Background : Nonalcoholic fatty liver diseases (NAFLD) is one of the main chronic liver diseases and raises the risk of morbidity and mortality due to its inevitable outcomes. Understanding the clinical manifestations of the liver is critical to identify NAFLD patients with the greatest risk of developing nonalcoholic steatohepatitis and cirrhosis. In the liver, C1q/TNF-related protein 1 (CTRP1) modulates both glucose and lipid metabolism and improves insulin sensitivity which may affect the pathologies of the liver. Materials and Methods : This study was conducted on 22 patients with NAFLD confirmed by ultrasonography and 21 healthy subjects. Clinical and histological variables were analyzed. The ultrasonography procedure was used to quantity Common bile duct (CBD). Liver stiffness (LS) was measured by transient elastography. Res ults : There was a significant difference in CTRP1 levels between NAFLD patients and controls (p=0.032). Moreover, there was a significant positive correlation between CTRP1 level and liver enzymes including AST (r=0.667; p=0.001), ALT (r=0.433; p=0.044), and γ-GT (r=0.428; p=0.047) in NAFLD patients. There was also a significant positive correlation between CTRP1 level and CBD (r= 0.469; p=0.028) in NAFLD patients. Moreover, the largest CBD was measured as 5.99 mm. Conclusion : It seems that CTRP1 is a novel adipokine related to the pathogenesis of NAFLD and is associated with the clinical manifestations of the liver such as liver enzymes, and CBD.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89495536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenosine deaminase (ADA) activity and isozymes in the serum of patients with hepatitis B compared with healthy people: a useful method in diagnosis clinical status","authors":"S. Athari","doi":"10.22037/AMLS.V3I1.17482","DOIUrl":"https://doi.org/10.22037/AMLS.V3I1.17482","url":null,"abstract":"Background: Adenosine deaminase (ADA) specifications (EC3.5.4.4) is an enzyme is involved in purine metabolism that breaks adenosine and deoxy-adenosine and produce inosine and deoxy-inosine and ammonia. The highest levels are of adenosine deaminase activity in monocytes and lymphocytes. High serum ADA activity with increased serum levels of AST, ALT and immunoglobulins were reported in variety of diseases including hepatitis. We aimed to investigate the activity of total ADA in serum of patients with hepatitis B and were determined molecular weight ADA1 and ADA2 isozymes in serum and RBC of hepatitis patients. Materials and Methods: We were defining experiments by electrophoresis on SDS-PAGE, for isozymes of ADA; ADA1 and ADA2 in serum and red blood cells. ADA1 molecular weight was estimated at about 35 KDa and ADA2 about 110 KDa. The total ADA activity was measured by the modified Ellis method in 37 patients with hepatitis B and 40 healthy controls in the age range (20-60 years). Results: The normal values for serum ADA in humans have been studied by various workers and found in serum samples to be in the range of 0-15 U/L, whiles in our analysis total ADA (tADA) enzyme activity is in controls and patients with hepatitis B, respectively, 13.35 ± 1.62 and 27.05 ± 8.49. Our results indicated that tADA level was higher in patients with hepatitis B than those of corresponding controls (P < 0.05).Total ADA enzyme activity shows a significant increase compared to the control group in all age groups tested. Conclusion: Therefore, the serum ADA level could be used as an index along with other parameters in follow up of patients with hepatitis B.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79393002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of pLLO vector encoding truncated form of Listeriolysin O as molecular adjuvant for DNA vaccine studies","authors":"M. Pouriayevali, T. Bamdad, S. M. Sadat, F. Sabahi, M. Aghasadeghi","doi":"10.22037/AMLS.V2I3.15464","DOIUrl":"https://doi.org/10.22037/AMLS.V2I3.15464","url":null,"abstract":"Background: The major problem of DNA vaccine is less immunogenicity of them verses other killed or live whole organism vaccines therefore adjuvants for use in this kind vaccines is very necessary. Genetic adjuvants with bacterial sources are an appropriate approach to modulate immune responses to DNA vaccines. Listeria Monocytogenes proteins such as Listeriolysin O (LLO) with CD4 and CD8 epitopes can be as an adjuvant to initiate both innate and adaptive immune responses if the protein cytotoxicity can be eliminated. Herein we constructed a truncated LLO plasmid as genetic adjuvant and tested it in combination with a DNA construct as a model vaccine. Materials and Methods: About 1340bp of the 5' end of whole LLO gene was amplified by PCR on DNA purified from Listeria Monocytogenes. Sequential sub cloning of truncated LLO into the Xho I/EcoRV sites of pcDNA3.1 plasmid, downstream of CMV promoter was done. pLLO plasmid was transfected to HEK293T cell line by lipofection method. LLO protein expression from transiently transfected 293T cell lysates was confirmed by western blotting. Then the adjuvant activity of LLO in BALB/c mice model was analyzed using proliferation test. Results: Double digestion of pLLO plasmid with the enzymes that were applied for cloning led to the isolation of two fragments with expected sizes. The final plasmid was also confirmed following sequencing reactions. Moreover, expression of LLO was evidenced in transfected 293T cells, compared to non-transfected controls. In vivo study was shown, high significant proliferative responses in LLO co-immunization pattern. Conclusion: In the DNA vaccine study, LLO co-administration plasmid could be a suitable genetic adjuvant to enhance cellular immune response of vaccine.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84666275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in-house indirect immunofluorescence test for ensuring that the cell bank is contamination free","authors":"M. Keshavarz, A. Shafiee, G. Tariqi, M. Izad, A. Yousefi, M. Hosseinzadeh","doi":"10.22037/AMLS.V1I3.11512","DOIUrl":"https://doi.org/10.22037/AMLS.V1I3.11512","url":null,"abstract":"Background: Cross-contamination between cells is a usual mistake at cell culture laboratories and cell banks worldwide. MRC5 diploid cell and Rk-13, Vero and Hela continuous cell lines are used in different stages of human viral vaccines propagation at Razi Vaccine and Serum Research Institute of Iran. However diploid and continuous cells are propagated at separated cell culture laboratory and continuous cells can contaminate MRC5 diploid cells. Therefore, a sensitive test is needed. World Health Organization recommends few molecular and cellular techniques to cell characterization. Materials and Methods: The present study was therefore designed to set up an indirect immunofluorescence (IIF) test as follows: Polyclonal anti-MRC5 cell and anti-rabbit antibodies were prepared in rabbit and goat, respectability. Anti-rabbit IgG was purified using protein G affinity chromatography, conjugated to fluorescein isothiocyanate (FITC), and then further purified to remove unbound FITC using Sephadex G 25 chromatography. Using double immunodiffusion assay, purification of homemade anti-rabbit IgG was asssayed by observation of a single arch. Results: The titer of homemade FITC conjugated goat anti rabbit IgG was measured 1/16 vs 1/8 of commercial type. Fluorescein/protein molar ratio of local made fluorescein goat anti-rabbit IgG was measured 3.44 and its protein concentration and FITC concentration were determined 1.198 mg/ml and 0.01 mg/ml, respectively. Conclusion: Moreover, homemade IIF test showed 100% intra-laboratory reproducibility. Purity of three batches of MRC5 working seed cell was verified using in- house IIF test and no contamination to continuous cell lines was found .","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"164 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80363721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}