Background and Aim: This study aimed to evaluate the protective effect of chicoric acid (CA) on mancozeb-induced male reproductive damage in mice. Methods: 65 NMRI male mice were randomly divided into 7 groups (n=8); 1: control group, 2: MZB–induced toxicity 3: MZB+ atropine (0.25 mg/kg daily), groups 4, 5, and 6: MZB+25, 50 and 100 mg/kg of CA respectively. 7: CA (100 mg). The mice were sacrificed thirty-five days later and blood and testis samples were obtained. Testosterone levels, sperm parameters, protamine deficiency, and sperm chromatin dispersion (SCD) were used to evaluate the reproductive system function. Results: The sperm count and sperm viability decreased in the MZB-intoxicated group; the sperm DNA fragmentation and protamine deficiency increased in this group. Head and neck deformity decreased in MZB+ CA groups (p<0.05). In MZB+ CA groups, the sperm motility type A, and B increased than the MZB group, abnormal sperm morphology within 100 mg/kg CA groups was less than 50 and 25 mg/kg. The groups pretreated with CA showed a significant increase in Leydig cells. Conclusion: The results revealed that chicoric acid has a protective effect on testis tissue damage induced by MZB. So, this is a promising therapeutic choice for the treatment of male infertility. *Corresponding Author: Hakimeh Akbari; Email: akbarih@gerums.ac.ir Please cite this article as: Forouzandeh H, Ahmadi I, Akbari H. The Protective Effect of Chicoric Acid on the Mancozeb-induced Male Reproductive Damage in Mice. Arch Med Lab Sci. 2021;7:1-10 (e6). https://doi.org/10.22037/amls.v7.33149
背景与目的:本研究旨在探讨菊苣酸(CA)对代铁锌致小鼠雄性生殖损伤的保护作用。方法:65只雄性NMRI小鼠随机分为7组(n=8);1:对照组，2:MZB诱导毒性3:MZB+阿托品(0.25 mg/kg每日)，4、5、6组:MZB+ CA 25、50、100 mg/kg。7: CA (100mg)。35天后处死小鼠，取血液和睾丸样本。睾酮水平、精子参数、鱼精蛋白缺乏和精子染色质离散度(SCD)被用来评估生殖系统功能。结果:mzb中毒组小鼠精子数量和精子活力下降;精子DNA断裂和鱼精蛋白缺乏症增加。MZB+ CA组头颈畸形明显减少(p<0.05)。MZB+ CA组A、B型精子活力较MZB组增加，100 mg/kg CA组内精子形态异常小于50、25 mg/kg。CA预处理组间质细胞数量显著增加。结论:菊苣酸对MZB致大鼠睾丸组织损伤具有保护作用。因此，这是治疗男性不育症的一个很有前途的治疗选择。*通讯作者:Hakimeh Akbari;Forouzandeh, Ahmadi I, Akbari H.菊酸对代谢物诱导的小鼠雄性生殖损伤的保护作用。中华医学杂志，2021;7:1-10(6)。https://doi.org/10.22037/amls.v7.33149
{"title":"The Protective Effect of Chicoric Acid on the Mancozeb-induced Male Reproductive Damage in Mice:","authors":"Hosein Forouzandeh, I. Ahmadi, H. Akbari","doi":"10.22037/AMLS.V7.33149","DOIUrl":"https://doi.org/10.22037/AMLS.V7.33149","url":null,"abstract":"Background and Aim: This study aimed to evaluate the protective effect of chicoric acid (CA) on mancozeb-induced male reproductive damage in mice. \u0000Methods: 65 NMRI male mice were randomly divided into 7 groups (n=8); 1: control group, 2: MZB–induced toxicity 3: MZB+ atropine (0.25 mg/kg daily), groups 4, 5, and 6: MZB+25, 50 and 100 mg/kg of CA respectively. 7: CA (100 mg). The mice were sacrificed thirty-five days later and blood and testis samples were obtained. Testosterone levels, sperm parameters, protamine deficiency, and sperm chromatin dispersion (SCD) were used to evaluate the reproductive system function. \u0000Results: The sperm count and sperm viability decreased in the MZB-intoxicated group; the sperm DNA fragmentation and protamine deficiency increased in this group. Head and neck deformity decreased in MZB+ CA groups (p<0.05). In MZB+ CA groups, the sperm motility type A, and B increased than the MZB group, abnormal sperm morphology within 100 mg/kg CA groups was less than 50 and 25 mg/kg. The groups pretreated with CA showed a significant increase in Leydig cells. \u0000Conclusion: The results revealed that chicoric acid has a protective effect on testis tissue damage induced by MZB. So, this is a promising therapeutic choice for the treatment of male infertility. \u0000*Corresponding Author: Hakimeh Akbari; Email: akbarih@gerums.ac.ir \u0000Please cite this article as: Forouzandeh H, Ahmadi I, Akbari H. The Protective Effect of Chicoric Acid on the Mancozeb-induced Male Reproductive Damage in Mice. Arch Med Lab Sci. 2021;7:1-10 (e6). https://doi.org/10.22037/amls.v7.33149","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84154434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sheida Behzadi Sheikhrobat, G. Kaydani, M. Makvandi, E. Rajaee, K. Angali
Background and Aim: Systemic lupus erythematosus (SLE) is an autoimmune disease and human polyomavirus BK (BKV) can be reactivated in patients with SLE due to the changes in the immune system and use of immunosuppressive drugs. In this study, we evaluated the prevalence of BKV infection among patients with SLE referred to Golestan hospital in Ahvaz, Iran between April 2013 to June 2016. Methods: In this cross-sectional study we studied 75 individuals including 40 patients with SLE and 35 normal individuals. Urine and blood samples were taken and DNA was extracted from urine and plasma. Polymerase Chain Reaction (PCR) test was used to detect the BKV genome and positive samples were sequenced to confirm BKV. BioEdit software and MEGA 6.0 software were used for phylogenetic analysis to assemble the viral genome. A phylogenetic tree was constructed by neighbor-joining analysis with 1,000 replicates of the bootstrap resampling test using Mega 6.0. Statistical analysis was done by SPSS version 22. Results: Among the 40 patients, 2 (5%) were men and 38 (95%) were women. The mean age of the patients was 39±10 years. 2.5% of plasma from patients with SLE were positive for BKV but none of the controls were positive in this regard.0% of control groups (p=0.346). Whereas in urine samples, 17.5% and 11.4% (p=0.458) of patients and the control group, were positive for BKV, respectively. However, there was no statistically significant difference between the patients and controls. Conclusion: BKV reactivation occurs in 17.5% of patients with SLE during immunosuppression therapy. Therefore, more studies on BKV DNA by highly sensitive molecular assays in Patients with SLE seem to be necessary. *Corresponding Author: Gholam Abbas Kaydani; Email: kaydani-ga@ajums.ac.ir Please cite this article as: Behzadi Sheikhrobat S, Kaydani GA, Makvandi M, Rajaee E, Ahmadi Angali K. The Frequency of Human Polyomavirus BK in Patients with Systemic Lupus Erythematosus: A Cross-Sectional Case-Control Study. Arch Med Lab Sci. 2021;7:1-6 (e5). https://doi.org/10.22037/amls.v7.33998
背景与目的:系统性红斑狼疮(SLE)是一种自身免疫性疾病，由于免疫系统的改变和免疫抑制药物的使用，人多瘤病毒BK (BKV)可在SLE患者中重新激活。在这项研究中，我们评估了2013年4月至2016年6月期间在伊朗阿瓦士Golestan医院转诊的SLE患者中BKV感染的流行情况。方法:在这个横断面研究中，我们研究了75个人，包括40例SLE患者和35例正常人。采集了尿液和血液样本，并从尿液和血浆中提取了DNA。采用聚合酶链反应(PCR)检测BKV基因组，对阳性样本进行测序，确认BKV。利用BioEdit软件和MEGA 6.0软件进行系统发育分析，组装病毒基因组。采用Mega 6.0软件对1000个重复的bootstrap重采样试验，通过邻联分析构建了系统发育树。采用SPSS 22进行统计分析。结果:40例患者中，男性2例(5%)，女性38例(95%)。患者平均年龄39±10岁。SLE患者的血浆中有2.5% BKV阳性，但对照组中没有一例BKV阳性。(p=0.346)。而在尿液样本中，患者和对照组分别有17.5%和11.4% (p=0.458)呈BKV阳性。然而，患者与对照组之间无统计学差异。结论:在免疫抑制治疗期间，17.5%的SLE患者发生BKV再激活。因此，有必要在SLE患者中开展更多的BKV DNA高灵敏度分子检测研究。*通讯作者:Gholam Abbas Kaydani;Behzadi Sheikhrobat S, Kaydani GA, Makvandi M, Rajaee E, Ahmadi Angali K.系统性红斑狼疮患者多瘤病毒BK的频率:一项横断病例对照研究。中华医学杂志，2021;7:1-6(5)。https://doi.org/10.22037/amls.v7.33998
{"title":"The Frequency of Human Polyomavirus BK in Patients with Systemic Lupus Erythematosus: A Cross-Sectional Case-Control Study","authors":"Sheida Behzadi Sheikhrobat, G. Kaydani, M. Makvandi, E. Rajaee, K. Angali","doi":"10.22037/AMLS.V7.33998","DOIUrl":"https://doi.org/10.22037/AMLS.V7.33998","url":null,"abstract":"Background and Aim: Systemic lupus erythematosus (SLE) is an autoimmune disease and human polyomavirus BK (BKV) can be reactivated in patients with SLE due to the changes in the immune system and use of immunosuppressive drugs. In this study, we evaluated the prevalence of BKV infection among patients with SLE referred to Golestan hospital in Ahvaz, Iran between April 2013 to June 2016. \u0000Methods: In this cross-sectional study we studied 75 individuals including 40 patients with SLE and 35 normal individuals. Urine and blood samples were taken and DNA was extracted from urine and plasma. Polymerase Chain Reaction (PCR) test was used to detect the BKV genome and positive samples were sequenced to confirm BKV. BioEdit software and MEGA 6.0 software were used for phylogenetic analysis to assemble the viral genome. A phylogenetic tree was constructed by neighbor-joining analysis with 1,000 replicates of the bootstrap resampling test using Mega 6.0. Statistical analysis was done by SPSS version 22. \u0000Results: Among the 40 patients, 2 (5%) were men and 38 (95%) were women. The mean age of the patients was 39±10 years. 2.5% of plasma from patients with SLE were positive for BKV but none of the controls were positive in this regard.0% of control groups (p=0.346). Whereas in urine samples, 17.5% and 11.4% (p=0.458) of patients and the control group, were positive for BKV, respectively. However, there was no statistically significant difference between the patients and controls. \u0000Conclusion: BKV reactivation occurs in 17.5% of patients with SLE during immunosuppression therapy. Therefore, more studies on BKV DNA by highly sensitive molecular assays in Patients with SLE seem to be necessary. \u0000*Corresponding Author: Gholam Abbas Kaydani; Email: kaydani-ga@ajums.ac.ir \u0000Please cite this article as: Behzadi Sheikhrobat S, Kaydani GA, Makvandi M, Rajaee E, Ahmadi Angali K. The Frequency of Human Polyomavirus BK in Patients with Systemic Lupus Erythematosus: A Cross-Sectional Case-Control Study. Arch Med Lab Sci. 2021;7:1-6 (e5). https://doi.org/10.22037/amls.v7.33998","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82202861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Esmael Amirazodi, G. Shamsaei, Shahram Rafie, Davood Kashipazha, Saeed Hesam
Background and Aim: Alteplase is a thrombolytic drug that is produced by recombinant DNA technology. Tissue plasminogen activator enzyme which converts plasminogen to the active form of plasmin is also produced by the same technology; it causes fibrinolysis and clot dissolution. This study aimed to compare the efficacy and complications of Alteplase injection in patients with acute ischemic stroke (AIS( during the first 3 hours and 3-4.5 hours after the onset of symptoms. Methods: In this study, patients with AIS who were referred to Golestan Hospital of Ahvaz city during 2018-2019 were selected. Information was collected by a checklist. Results: The results showed that the mean Modified Rankin Scale (mRS) for 3 months and 6 months (p-value: 0.91 for 3 months and p-value: 0.80 for 6 months) and National Institutes of Health Stroke Scale (NIHSS) (p-value: 0.21) were not significantly different between both groups; statistically, no significant relationship was observed between them. The incidence of complications after treatment was almost similar, in both groups. Conclusion: Finally, it was concluded that complications and efficacy of rt-PA (Alteplase) injection were not statistically different, between the two groups under study. *Corresponding Author: Gholamreza Shamsaei; Email: grshamsaei@gmail.com; shamsaei-gh@ajums.ac.ir Please cite this article as: Amirazodi E, Shamsaei G, Rafie S, Kashipazha D, Hesam S. Comparison of Efficacy and Complication of Alteplase Injection in Acute Ischemic Stroke. Arch Med Lab Sci. 2021;7:1-6 (e4). https://doi.org/10.22037/amls.v7.33501
{"title":"Comparison of Efficacy and Complication of Alteplase Injection in Acute Ischemic Stroke","authors":"Esmael Amirazodi, G. Shamsaei, Shahram Rafie, Davood Kashipazha, Saeed Hesam","doi":"10.22037/AMLS.V7.33501","DOIUrl":"https://doi.org/10.22037/AMLS.V7.33501","url":null,"abstract":"Background and Aim: Alteplase is a thrombolytic drug that is produced by recombinant DNA technology. Tissue plasminogen activator enzyme which converts plasminogen to the active form of plasmin is also produced by the same technology; it causes fibrinolysis and clot dissolution. This study aimed to compare the efficacy and complications of Alteplase injection in patients with acute ischemic stroke (AIS( during the first 3 hours and 3-4.5 hours after the onset of symptoms. \u0000Methods: In this study, patients with AIS who were referred to Golestan Hospital of Ahvaz city during 2018-2019 were selected. Information was collected by a checklist. \u0000Results: The results showed that the mean Modified Rankin Scale (mRS) for 3 months and 6 months (p-value: 0.91 for 3 months and p-value: 0.80 for 6 months) and National Institutes of Health Stroke Scale (NIHSS) (p-value: 0.21) were not significantly different between both groups; statistically, no significant relationship was observed between them. The incidence of complications after treatment was almost similar, in both groups. \u0000Conclusion: Finally, it was concluded that complications and efficacy of rt-PA (Alteplase) injection were not statistically different, between the two groups under study. \u0000*Corresponding Author: Gholamreza Shamsaei; Email: grshamsaei@gmail.com; shamsaei-gh@ajums.ac.ir \u0000Please cite this article as: Amirazodi E, Shamsaei G, Rafie S, Kashipazha D, Hesam S. Comparison of Efficacy and Complication of Alteplase Injection in Acute Ischemic Stroke. Arch Med Lab Sci. 2021;7:1-6 (e4). https://doi.org/10.22037/amls.v7.33501","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81781551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Aim: Phage display technology provides a new approach for making human antibody fragments that could be applicable in passive immune therapy. We applied the use of this technology to make human single-chain variable fragments (scFvs) specific for tetanus toxin. Tetanus toxin is a neurotoxin constituted by the association of two subunits, mediates its lethal action by blocking neuromuscular vesicle docking. Methods: We previously found that six Human scFv clones inhibit toxin binding to ganglioside GT1b. This is the final report of human tetanus scFvs (scFv 8 and scFv 13) isolated from an immunized library of more than 106 scFv clones with in vivo neutralizing activity. Results: Only scFv 13 can reduce the in vivo toxicity induced by tetanus toxin. Also, scFv 8 has a weak capability of reducing the in vivo toxicity of the toxin. Conclusion: These selected ScFvs can be considered as a possible option to substitute the human tetanus immunoglobulin (HTIG) which is extensively current immunotherapy for tetanus patients. Taken together, our results suggest that the use of human tetanus scFvs may lead to a less aggressive passive immune therapy against tetanus. *Corresponding Author: Mahdi Aminian; Email: amminian@tums.ac.ir Please cite this article as: Khalili E, Abbasi E, Aminian M. Neutralization of Lethal Potency of Tetanus Toxin using Phage Display Produced ScFv Antibody.Arch Med Lab Sci. 2021;7:(e3). https://doi.org/10.22037/amls.v7.33786
背景与目的:噬菌体展示技术为制备可用于被动免疫治疗的人抗体片段提供了新的途径。我们应用该技术制备了破伤风毒素特异性的人单链可变片段(scFvs)。破伤风毒素是一种由两个亚基结合而成的神经毒素，通过阻断神经肌肉囊泡对接来介导其致死性作用。方法:我们先前发现6个人类scFv克隆抑制毒素与神经节苷脂GT1b的结合。这是从超过106个具有体内中和活性的人破伤风单链病毒(scFv 8和scFv 13)免疫文库中分离得到的最终报告。结果:仅scfv13能降低破伤风毒素的体内毒性。同时，scfv8降低毒素体内毒性的能力较弱。结论:经筛选的ScFvs可作为替代目前广泛用于破伤风患者免疫治疗的人破伤风免疫球蛋白(HTIG)的可能选择。综上所述，我们的研究结果表明，使用人破伤风scFvs可能导致对破伤风的被动免疫治疗不那么积极。*通讯作者:Mahdi Aminian;电子邮件:amminian@tums.ac.ir请引用这篇文章:Khalili E, Abbasi E, Aminian M.利用噬菌体展示产生的ScFv抗体中和破伤风毒素致死效力。中华医学杂志，2021;7:(3)。https://doi.org/10.22037/amls.v7.33786
{"title":"Neutralization of Lethal Potency of Tetanus Toxin using Phage Display Produced scFv Antibody","authors":"E. Khalili, E. Abbasi, M. Aminian","doi":"10.22037/AMLS.V7.33786","DOIUrl":"https://doi.org/10.22037/AMLS.V7.33786","url":null,"abstract":"Background and Aim: Phage display technology provides a new approach for making human antibody fragments that could be applicable in passive immune therapy. We applied the use of this technology to make human single-chain variable fragments (scFvs) specific for tetanus toxin. Tetanus toxin is a neurotoxin constituted by the association of two subunits, mediates its lethal action by blocking neuromuscular vesicle docking. \u0000Methods: We previously found that six Human scFv clones inhibit toxin binding to ganglioside GT1b. This is the final report of human tetanus scFvs (scFv 8 and scFv 13) isolated from an immunized library of more than 106 scFv clones with in vivo neutralizing activity. \u0000Results: Only scFv 13 can reduce the in vivo toxicity induced by tetanus toxin. Also, scFv 8 has a weak capability of reducing the in vivo toxicity of the toxin. \u0000Conclusion: These selected ScFvs can be considered as a possible option to substitute the human tetanus immunoglobulin (HTIG) which is extensively current immunotherapy for tetanus patients. Taken together, our results suggest that the use of human tetanus scFvs may lead to a less aggressive passive immune therapy against tetanus. \u0000*Corresponding Author: Mahdi Aminian; Email: amminian@tums.ac.ir \u0000Please cite this article as: Khalili E, Abbasi E, Aminian M. Neutralization of Lethal Potency of Tetanus Toxin using Phage Display Produced ScFv Antibody.Arch Med Lab Sci. 2021;7:(e3). https://doi.org/10.22037/amls.v7.33786","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"189 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79763940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The coronavirus epidemic has become one of the major health concerns all over the world recently. Like other strains of coronavirus, this strain also spreads through a droplet-based transmission that is the main cause of its worldwide spread. Several trials of antiviral medicines related to the control of the virus have already begun globally but still one of the main problems is the lack of a viable treatment option. An extensive amount of research is still taking place to organize the data associated with genomics and proteomics of its original strain SARS-CoV-2 alongside other mutant strains. This review summarizes the related up-to-date research that is going on the structural organization of the genome and proteome of the virus. *Corresponding Author: Ehsan Ul Haq; Email: ehsanulhaqsani5@gmail.com; BBTF17E116@uos.edu.pk Please cite this article as: Ul Haq E. Deep Characterization of SARS-CoV-2: An Overview. Arch Med Lab Sci. 2021;7:1-6 (e2). https://doi.org/10.22037/amls.v7.33616
最近，冠状病毒疫情已成为全球主要的健康问题之一。与其他冠状病毒一样，这种病毒也通过基于飞沫的传播传播，这是其全球传播的主要原因。与控制病毒有关的几项抗病毒药物试验已经在全球开始，但主要问题之一仍然是缺乏可行的治疗方案。目前仍在进行大量的研究，以组织与其原始菌株SARS-CoV-2以及其他突变菌株的基因组学和蛋白质组学相关的数据。本文综述了近年来有关病毒基因组和蛋白质组结构组织的研究进展。*通讯作者:Ehsan Ul Haq;电子邮件:ehsanulhaqsani5@gmail.com;ulhaq E. SARS-CoV-2的深度表征:综述。中华医学杂志，2021;7:1-6 (e2)。https://doi.org/10.22037/amls.v7.33616
{"title":"Deep Characterization of SARS-CoV-2: An Overview","authors":"E. Haq","doi":"10.22037/AMLS.V7.33616","DOIUrl":"https://doi.org/10.22037/AMLS.V7.33616","url":null,"abstract":"The coronavirus epidemic has become one of the major health concerns all over the world recently. Like other strains of coronavirus, this strain also spreads through a droplet-based transmission that is the main cause of its worldwide spread. Several trials of antiviral medicines related to the control of the virus have already begun globally but still one of the main problems is the lack of a viable treatment option. An extensive amount of research is still taking place to organize the data associated with genomics and proteomics of its original strain SARS-CoV-2 alongside other mutant strains. This review summarizes the related up-to-date research that is going on the structural organization of the genome and proteome of the virus. \u0000*Corresponding Author: Ehsan Ul Haq; Email: ehsanulhaqsani5@gmail.com; BBTF17E116@uos.edu.pk \u0000Please cite this article as: Ul Haq E. Deep Characterization of SARS-CoV-2: An Overview. Arch Med Lab Sci. 2021;7:1-6 (e2). https://doi.org/10.22037/amls.v7.33616","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90846297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Azad, Mohsen Zargar, M. Zolfaghari, A. Mohammadbeigi
Background and Aim: Hepatitis B virus (HBV) is a human carcinogenesis agent. Interleukin 10 (IL-10) is a key anti-inflammatory cytokine, and single nucleotide polymorphisms in IL-10 gene promoter are correlated with infections caused by HBV. This research intended to assess the prevalence and genotype of HBV as well as the association between the polymorphisms of -819 and -1082 in the IL-10 gene with HBV in individuals with HBV infection in Qom Province, Iran Methods: In this cross-sectional research, 360 individuals with chronic HBV infection and control group were involved between July 2018 and March 2019. HBV diagnosis was evaluated using ELISA and nested PCR assays. To determine polymorphisms in the IL-10 gene promoter in HBV positive and control samples, an allele-specific polymerase chain reaction technique was employed. Results: The constructed phylogenetic trees for the HBsAg gene revealed that all sequences under study belong to genotype D and also, the majority of HBV samples presented similar sequences to the Iranian samples. Genotype frequencies of TT, TC and CC (polymorphism -819) were 82.2%, 11.6% and 6.1% for patients and 85%, 10.5% and 4.4% in control groups, respectively. Also, frequency of genotypes of AA, AG and GG (polymorphism -1082) were 45%, 43.8% and 11.1% for patients and 42.2%, 46.1% and 11.6% in control groups, respectively. Conclusion: Here, we found no association among IL-10 gene polymorphisms in control and HBV-infected groups. However, more studies about the frequency of chronic HBV infection are necessary to be conducted. *Corresponding Author: Mohsen Zargar; Email: zargar@qom-iau.ac.ir Interleukins-10 Gene Polymorphism and Hepatitis B Virus: Evidence in Iran. Arch Med Lab Sci. 2021;7:1-7 (e7). https://doi.org/10.22037/amls.v7.33557
{"title":"The Association between Interleukin-10 Gene Polymorphisms and Hepatitis B Virus: Evidence in Iran","authors":"A. Azad, Mohsen Zargar, M. Zolfaghari, A. Mohammadbeigi","doi":"10.22037/AMLS.V7.33557","DOIUrl":"https://doi.org/10.22037/AMLS.V7.33557","url":null,"abstract":"Background and Aim: Hepatitis B virus (HBV) is a human carcinogenesis agent. Interleukin 10 (IL-10) is a key anti-inflammatory cytokine, and single nucleotide polymorphisms in IL-10 gene promoter are correlated with infections caused by HBV. This research intended to assess the prevalence and genotype of HBV as well as the association between the polymorphisms of -819 and -1082 in the IL-10 gene with HBV in individuals with HBV infection in Qom Province, Iran \u0000Methods: In this cross-sectional research, 360 individuals with chronic HBV infection and control group were involved between July 2018 and March 2019. HBV diagnosis was evaluated using ELISA and nested PCR assays. To determine polymorphisms in the IL-10 gene promoter in HBV positive and control samples, an allele-specific polymerase chain reaction technique was employed. \u0000Results: The constructed phylogenetic trees for the HBsAg gene revealed that all sequences under study belong to genotype D and also, the majority of HBV samples presented similar sequences to the Iranian samples. Genotype frequencies of TT, TC and CC (polymorphism -819) were 82.2%, 11.6% and 6.1% for patients and 85%, 10.5% and 4.4% in control groups, respectively. Also, frequency of genotypes of AA, AG and GG (polymorphism -1082) were 45%, 43.8% and 11.1% for patients and 42.2%, 46.1% and 11.6% in control groups, respectively. \u0000Conclusion: Here, we found no association among IL-10 gene polymorphisms in control and HBV-infected groups. However, more studies about the frequency of chronic HBV infection are necessary to be conducted. \u0000*Corresponding Author: Mohsen Zargar; Email: zargar@qom-iau.ac.ir \u0000Interleukins-10 Gene Polymorphism and Hepatitis B Virus: Evidence in Iran. Arch Med Lab Sci. 2021;7:1-7 (e7). https://doi.org/10.22037/amls.v7.33557","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80289386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Aim: Scleroderma (SC) is a connective tissue disease, characterized by diffuse microangiopathy and excessive production of collagen. The current study aimed to investigate the effectiveness of Grapex extract in improving the wound of patients with scleroderma. Methods: This randomized controlled double-blind clinical trial was performed from 2018 to 2019 on patients with scleroderma referred to Golestan Hospital in Ahvaz, Iran. Forty patients with active SC were selected and randomly divided into two groups. Patients applied the ointment twice a day for 4 weeks on the surface of their wounds. After four weeks of using the cream, the rate of wound healing was determined by clinical examination of the wounds. Results: 6 people were excluded from the study due to the lack of referral and final analyzes were performed on 34 patients (16 patients in the control group and 18 patients in the case group). The results of this study showed that there was a significant difference between the two groups in terms of response to treatment (p <0.0001). At the end of the fourth week, 88.89% of the patients in the case group (16 of the 18 patients) achieved complete healing of the wounds in comparison with 18.75% of the control group (3 of the 16 patients). Neither the control group nor the case group had a significant association between response to treatment with age and gender of patients, type of scleroderma, duration of illness, and symptoms. Conclusion: This study showed the effectiveness of Grapex cream ointment in healing scleroderma wounds. Therefore, Grapex cream is an effective, inexpensive, safe, and available medicine that can be used to accelerate wound healing in patients with scleroderma. *Corresponding Authors: Elham Rajaei, Email: elhamrj@gmail.com Please cite this article as: Hemmati AA, Deris Zayeri Z, Rajaei E, Ghanavati M. The Effect of Grapex on Wounds Healing in Patients with Scleroderma: A Randomized Controlled Clinical Trial. Arch Med Lab Sci. 2021;7:1-10 (e1). https://doi.org/10.22037/amls.v7.31059
{"title":"The Effect of Grapex on Wounds Healing in Patients with Scleroderma: A Randomized Controlled Clinical Trial","authors":"A. Hemmati, Z. Zayeri, Elham Rajaei, M. Ghanavati","doi":"10.22037/AMLS.V7.31059","DOIUrl":"https://doi.org/10.22037/AMLS.V7.31059","url":null,"abstract":"Background and Aim: Scleroderma (SC) is a connective tissue disease, characterized by diffuse microangiopathy and excessive production of collagen. The current study aimed to investigate the effectiveness of Grapex extract in improving the wound of patients with scleroderma. \u0000Methods: This randomized controlled double-blind clinical trial was performed from 2018 to 2019 on patients with scleroderma referred to Golestan Hospital in Ahvaz, Iran. Forty patients with active SC were selected and randomly divided into two groups. Patients applied the ointment twice a day for 4 weeks on the surface of their wounds. After four weeks of using the cream, the rate of wound healing was determined by clinical examination of the wounds. \u0000Results: 6 people were excluded from the study due to the lack of referral and final analyzes were performed on 34 patients (16 patients in the control group and 18 patients in the case group). The results of this study showed that there was a significant difference between the two groups in terms of response to treatment (p <0.0001). At the end of the fourth week, 88.89% of the patients in the case group (16 of the 18 patients) achieved complete healing of the wounds in comparison with 18.75% of the control group (3 of the 16 patients). Neither the control group nor the case group had a significant association between response to treatment with age and gender of patients, type of scleroderma, duration of illness, and symptoms. \u0000Conclusion: This study showed the effectiveness of Grapex cream ointment in healing scleroderma wounds. Therefore, Grapex cream is an effective, inexpensive, safe, and available medicine that can be used to accelerate wound healing in patients with scleroderma. \u0000*Corresponding Authors: Elham Rajaei, Email: elhamrj@gmail.com \u0000Please cite this article as: Hemmati AA, Deris Zayeri Z, Rajaei E, Ghanavati M. The Effect of Grapex on Wounds Healing in Patients with Scleroderma: A Randomized Controlled Clinical Trial. Arch Med Lab Sci. 2021;7:1-10 (e1). https://doi.org/10.22037/amls.v7.31059","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"167 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76179315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pouyan Malekinezhad, N. Afzali, S. H. Farhangfar, A. Omidi, A. Mohammadi
Background and Aim: Mycotoxin-contaminated feed causes significant concern in poultry production and public health because of serious economic losses and health problems caused by them. Berberine hydrochloride (Berberine), a natural plant alkaloid derived from Chinese medicine, is characterized by diverse pharmacological effects. This study is designed to evaluate the effects of different levels of Berberine (BBR) on carcass traits and meat quality of broilers fed diets contaminated with Aflatoxin B1 (AFB) and Ochratoxin A (OCT). Methods: A 42-day floor pen trial was performed with 288 Ross 308 broilers. A randomized design, with 4 replicates of 8 birds each, was conducted with the following 9 treatments: (1) negative control diet with no additives (NC); (2) NC + 2 ppm AFB (positive control AFB; PCAFB); (3) NC + 2 ppm OCT (positive control OCT; PCOCT); (4) PCAFB + 200 mg/kg BBR; (5) PCAFB + 400 mg/kg BBR; (6) PCAFB + 600 mg/kg BBR; (7) PCOCT + 200 mg/kg BBR; (8) PCOCT + 400 mg/kg BBR; and (9) PCOCT + 600 mg/kg BBR. At the end of the experiment, from every group, eight birds were selected, slaughtered, and subjected to analyses. The analyzed parameters carcass and cut yields and meat quality according to water-holding capacity (WHC) and breast level MDA. Results: Both PCAFB and PCOTA diets decreased carcass relative weight, breast level MDA, and WHC values compared with the NC diet (p 0.05). Carcass efficiency in PCAFB and PCOTA treatments was lower than in the NC treatment (p˂0.05), and supplementation with BBR at multiple levels partially or fully reversed these effects. The addition of 600 mg/kg BBR to PCAFB diet increased carcass relative weight compared to PCAFB alone (p>0.05). Conclusion: These data provided supplementation of BBR (600 mg/kg) improves meat quality in broiler fed diet contaminated with mycotoxins. *Corresponding Author: Nazar Afzali; Email: nafzali@birjand.ac.ir Please cite this article as: Malekinezhad P, Afzali N, Farhangfar SH, Omidi A, Mohammadi A. Berberine Improves Meat Quality and Carcass Traits in Broilers Challenged with Mycotoxins. Arch Med Lab Sci. 2020;6:1-9 (e23). https://doi.org/10.22037/amls.v6.33330
{"title":"Berberine Improves Meat Quality and Carcass Traits in Broilers Challenged with Mycotoxins","authors":"Pouyan Malekinezhad, N. Afzali, S. H. Farhangfar, A. Omidi, A. Mohammadi","doi":"10.22037/AMLS.V6.33330","DOIUrl":"https://doi.org/10.22037/AMLS.V6.33330","url":null,"abstract":"Background and Aim: Mycotoxin-contaminated feed causes significant concern in poultry production and public health because of serious economic losses and health problems caused by them. Berberine hydrochloride (Berberine), a natural plant alkaloid derived from Chinese medicine, is characterized by diverse pharmacological effects. This study is designed to evaluate the effects of different levels of Berberine (BBR) on carcass traits and meat quality of broilers fed diets contaminated with Aflatoxin B1 (AFB) and Ochratoxin A (OCT). \u0000Methods: A 42-day floor pen trial was performed with 288 Ross 308 broilers. A randomized design, with 4 replicates of 8 birds each, was conducted with the following 9 treatments: (1) negative control diet with no additives (NC); (2) NC + 2 ppm AFB (positive control AFB; PCAFB); (3) NC + 2 ppm OCT (positive control OCT; PCOCT); (4) PCAFB + 200 mg/kg BBR; (5) PCAFB + 400 mg/kg BBR; (6) PCAFB + 600 mg/kg BBR; (7) PCOCT + 200 mg/kg BBR; (8) PCOCT + 400 mg/kg BBR; and (9) PCOCT + 600 mg/kg BBR. At the end of the experiment, from every group, eight birds were selected, slaughtered, and subjected to analyses. The analyzed parameters carcass and cut yields and meat quality according to water-holding capacity (WHC) and breast level MDA. \u0000Results: Both PCAFB and PCOTA diets decreased carcass relative weight, breast level MDA, and WHC values compared with the NC diet (p 0.05). Carcass efficiency in PCAFB and PCOTA treatments was lower than in the NC treatment (p˂0.05), and supplementation with BBR at multiple levels partially or fully reversed these effects. The addition of 600 mg/kg BBR to PCAFB diet increased carcass relative weight compared to PCAFB alone (p>0.05). \u0000Conclusion: These data provided supplementation of BBR (600 mg/kg) improves meat quality in broiler fed diet contaminated with mycotoxins. \u0000*Corresponding Author: Nazar Afzali; Email: nafzali@birjand.ac.ir \u0000Please cite this article as: Malekinezhad P, Afzali N, Farhangfar SH, Omidi A, Mohammadi A. Berberine Improves Meat Quality and Carcass Traits in Broilers Challenged with Mycotoxins. Arch Med Lab Sci. 2020;6:1-9 (e23). https://doi.org/10.22037/amls.v6.33330","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"6 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81863081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thalassemia is a multisystemic disease in the field of hemolysis and chronic anemia caused by the erythropoietic disorder. The severe effects of iron overload from continuous blood transfusion iron chelators side effects, and involvement of multiple organs in thalassemias such as heart failure, liver, and endocrine dysfunction can all affect kidney function. Although there has been much debate about changes in renal function in thalassemia for many years, the presence of hyperfiltration and ultimately, decreased renal function in almost all studies. It seems for the researchers to look beyond kidney function in a thalassemia perspective, because of secretory biomarkers of proximal tubular renal cells that are sensitive to pathologic agents, which may be a good indicator of the courses of treatment and prognosis of patients. Future studies will be sooner or later. *Corresponding Author: Malihe Najafpour; Email: malihe_najafpour@modares.ac.ir Please cite this article as: Malaki M, Najafpour M, Talebi M, Azimi A. Change in Attitude in Renal Function in Major Beta Thalassemia. Arch Med Lab Sci. 2020;6:1-5 (e24). https://doi.org/10.22037/amls.v6.33057
地中海贫血是一种由红细胞生成障碍引起的溶血和慢性贫血的多系统疾病。持续输血引起的铁超载的严重影响，铁螯合剂的副作用，以及地中海贫血患者累及多器官，如心力衰竭、肝脏和内分泌功能紊乱，都可能影响肾功能。尽管多年来关于地中海贫血患者的肾功能变化一直存在很多争论，但几乎所有的研究都表明存在超滤过并最终导致肾功能下降。由于近端肾小管细胞的分泌性生物标志物对病理药物敏感，这可能是治疗过程和患者预后的一个很好的指标，因此研究人员似乎从地中海贫血的角度看待肾脏功能之外的问题。未来的研究是迟早的事。*通讯作者:Malihe Najafpour;Malaki M, Najafpour M, Talebi M, Azimi A.重度β地中海贫血患者的肾功能改变。中华医学杂志，2020;6:1-5 (e24)。https://doi.org/10.22037/amls.v6.33057
{"title":"Change in Attitude in Renal Function in Major Beta Thalassemia","authors":"M. Malaki, Malihe Najafpour, M. Talebi, Ako Azimi","doi":"10.22037/AMLS.V6.33057","DOIUrl":"https://doi.org/10.22037/AMLS.V6.33057","url":null,"abstract":"Thalassemia is a multisystemic disease in the field of hemolysis and chronic anemia caused by the erythropoietic disorder. The severe effects of iron overload from continuous blood transfusion iron chelators side effects, and involvement of multiple organs in thalassemias such as heart failure, liver, and endocrine dysfunction can all affect kidney function. Although there has been much debate about changes in renal function in thalassemia for many years, the presence of hyperfiltration and ultimately, decreased renal function in almost all studies. It seems for the researchers to look beyond kidney function in a thalassemia perspective, because of secretory biomarkers of proximal tubular renal cells that are sensitive to pathologic agents, which may be a good indicator of the courses of treatment and prognosis of patients. Future studies will be sooner or later. \u0000*Corresponding Author: Malihe Najafpour; Email: malihe_najafpour@modares.ac.ir \u0000Please cite this article as: Malaki M, Najafpour M, Talebi M, Azimi A. Change in Attitude in Renal Function in Major Beta Thalassemia. Arch Med Lab Sci. 2020;6:1-5 (e24). https://doi.org/10.22037/amls.v6.33057","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"873 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82536066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Rostami, Vajiheh Najafi, Vahideh Behmard, Mohammad Panji, Fatemeh Sadat Moravej, Sina Dalvand, A. Namdari, Negar Yavari, Zakieh Sadat Sheikhalishahi
Background and Aim: The prevalence of type 2 diabetes mellitus (T2DM) is increasing globally. There is increasing evidence in the correlation between altered vitamin D levels, thyroid dysfunction, and T2DM. The goal of this study was to evaluate the association between serum 25-hydroxy vitamin D (25(OH) D), lipid profile, glucose and thyroid-related parameters among patients with T2DM and non-diabetic individuals. Methods: This case-control study was conducted on 228 individuals (110 type two diabetics and 118 healthy controls). The serum concentration of 25(OH) D was determined by chemiluminescence assay. Photometric methods measured serum levels of fasting blood glucose (FBG), calcium, phosphorous, total Cholesterol (TC), triacylglyceride (TG), high-density lipoprotein (HDL). Low-density lipoprotein (LDL) and VLDL levels were estimated from the Friedewald equation (LDL-C=TC–HDL-C–(TG/5)). The Elisa kit measured serum T4 and TSH. Results: 80% of Patients with T2DM and 71% of healthy individuals were suffering from vitamin D insufficiency. A non-significant relationship between TG (P=0.36), HDL (P=0.33), VLDL (P=0.36), T4 (P=0.56) calcium (P=0.39) and phosphorus (P=0.41) levels were showed in control and diabetic groups. The levels of FBG (P=0.000), TC (P=0.001), LDL (P=0.004), TSH (P=0.000) were significantly higher, and the levels of 25 (OH) D (P=0.001) was significantly lower in the T2DM group compared to the non-diabetic group. Conclusion: We revealed that the serum level of 25 (OH) D is lower in patients with T2DM, and the TSH level is significantly higher compared to the non-diabetic group. Thyroid dysfunction and a low level of 25 (OH) D are associated with a high risk of T2DM. *Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: z.sheikhalishahi@gmail.com Please cite this article as: Rostami E, Najafi V, Behmard V, Panji M, Moravej FS, Dalvand S, Namdari A, Yavari N, Sheikhalishahi ZS. Evaluation of 25-hydroxy Vitamin D Serum Levels and Thyroid-related Parameters in Patients with Type 2 Diabetes Mellitus and Healthy People in Shiraz, Iran. Arch Med Lab Sci. 2020;6:1-6 (e22). https://doi.org/10.22037/amls.v6.31301
背景与目的:2型糖尿病(T2DM)的患病率在全球范围内呈上升趋势。越来越多的证据表明维生素D水平改变、甲状腺功能障碍和2型糖尿病之间存在相关性。本研究的目的是评估T2DM和非糖尿病患者血清25-羟基维生素D (25(OH) D)、血脂、血糖和甲状腺相关参数之间的关系。方法:对228例患者进行病例对照研究，其中2型糖尿病患者110例，健康对照118例。化学发光法测定血清25(OH) D浓度。光度法测定空腹血糖(FBG)、钙、磷、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)的血清水平。根据Friedewald方程(LDL- c = TC-HDL-C - (TG/5))估算低密度脂蛋白(LDL)和VLDL水平。Elisa试剂盒检测血清T4和TSH。结果:80%的T2DM患者和71%的健康人存在维生素D不足。对照组与糖尿病组TG (P=0.36)、HDL (P=0.33)、VLDL (P=0.36)、T4 (P=0.56)、钙(P=0.39)、磷(P=0.41)水平无显著相关。T2DM组FBG (P=0.000)、TC (P=0.001)、LDL (P=0.004)、TSH (P=0.000)水平显著高于非糖尿病组，25 (OH) D水平显著低于非糖尿病组(P=0.001)。结论:我们发现T2DM患者血清25 (OH) D水平较低，TSH水平明显高于非糖尿病组。甲状腺功能障碍和低水平的25 (OH) D与T2DM的高风险相关。*通讯作者:Zakieh Sadat Sheikhalishahi;Rostami E, Najafi V, Behmard V, Panji M, Moravej FS, Dalvand S, Namdari A, Yavari N, Sheikhalishahi ZS。伊朗设拉子地区2型糖尿病患者和健康人血清25-羟基维生素D水平及甲状腺相关参数的评价中华医学杂志，2020;6:1-6 (e22)。https://doi.org/10.22037/amls.v6.31301
{"title":"Evaluation of 25-hydroxy Vitamin D Serum Levels and Thyroid-related Parameters in Patients with Type 2 Diabetes Mellitus and Healthy People in Shiraz, Iran","authors":"E. Rostami, Vajiheh Najafi, Vahideh Behmard, Mohammad Panji, Fatemeh Sadat Moravej, Sina Dalvand, A. Namdari, Negar Yavari, Zakieh Sadat Sheikhalishahi","doi":"10.22037/AMLS.V6.31301","DOIUrl":"https://doi.org/10.22037/AMLS.V6.31301","url":null,"abstract":"Background and Aim: The prevalence of type 2 diabetes mellitus (T2DM) is increasing globally. There is increasing evidence in the correlation between altered vitamin D levels, thyroid dysfunction, and T2DM. The goal of this study was to evaluate the association between serum 25-hydroxy vitamin D (25(OH) D), lipid profile, glucose and thyroid-related parameters among patients with T2DM and non-diabetic individuals. \u0000Methods: This case-control study was conducted on 228 individuals (110 type two diabetics and 118 healthy controls). The serum concentration of 25(OH) D was determined by chemiluminescence assay. Photometric methods measured serum levels of fasting blood glucose (FBG), calcium, phosphorous, total Cholesterol (TC), triacylglyceride (TG), high-density lipoprotein (HDL). Low-density lipoprotein (LDL) and VLDL levels were estimated from the Friedewald equation (LDL-C=TC–HDL-C–(TG/5)). The Elisa kit measured serum T4 and TSH. \u0000Results: 80% of Patients with T2DM and 71% of healthy individuals were suffering from vitamin D insufficiency. A non-significant relationship between TG (P=0.36), HDL (P=0.33), VLDL (P=0.36), T4 (P=0.56) calcium (P=0.39) and phosphorus (P=0.41) levels were showed in control and diabetic groups. The levels of FBG (P=0.000), TC (P=0.001), LDL (P=0.004), TSH (P=0.000) were significantly higher, and the levels of 25 (OH) D (P=0.001) was significantly lower in the T2DM group compared to the non-diabetic group. \u0000Conclusion: We revealed that the serum level of 25 (OH) D is lower in patients with T2DM, and the TSH level is significantly higher compared to the non-diabetic group. Thyroid dysfunction and a low level of 25 (OH) D are associated with a high risk of T2DM. \u0000*Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: z.sheikhalishahi@gmail.com \u0000Please cite this article as: Rostami E, Najafi V, Behmard V, Panji M, Moravej FS, Dalvand S, Namdari A, Yavari N, Sheikhalishahi ZS. Evaluation of 25-hydroxy Vitamin D Serum Levels and Thyroid-related Parameters in Patients with Type 2 Diabetes Mellitus and Healthy People in Shiraz, Iran. Arch Med Lab Sci. 2020;6:1-6 (e22). https://doi.org/10.22037/amls.v6.31301","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85208281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：