Roya Bazzaz, P. Yaghmaei, S. Dastmalchi, N. Rashtchizadeh
Background and Aim: Combination therapy is one of the new strategies that minimize resistance to chemotherapy and reduces drug toxicity. Here, we investigated the effect of combination therapy with 5-Fluorouracil and Gamma Tocopherol on cell survival and BAX/BCL2 gene expression ratio in HT29 colon cancer cells. Methods: The proliferation of cancer cells was determined via colony formation assay. BAX/BCL2 ratio was evaluated after incubation with concentrations of 5-Fluorouracil and Gamma Tocopherol via real-time-PCR. Results: The average number of colonies in the cells treated with 5-Fluorouracil, Gamma Tocopherol and their combination of them was 63±4, 78±3, and 28±2, respectively which significantly decreased in the combination group. In contrast with the control group, the BAX/BCL2 ratio remarkably increased when the cell underwent combinational treatment (p<0.05). Conclusion: 5-Fluorouracil and Gamma Tocopherol reduced HT 29 cell proliferation. Our results suggest that combination therapy with 5- Fluorouracil and Gamma Tocopherol can be considered as a strategy for induction of apoptosis via increasing the BAX/BCL2 ratio. *Corresponding Author: Nadereh Rashtchizadeh; Email: rashtchizadeh@rocketmail.com Please cite this article as: Bazzaz R, Yaghmaei P, Dastmalchi S, Rashtchizadeh N. Apoptosis Induction with Enhancement of BAX/BCL2 Gene Expression Ratio via Combination Therapy in HT29 Colon Cancer Cells. Arch Med Lab Sci. 2020;6:1-7 (e21). https://doi.org/10.22037/amls.v6.33489
{"title":"Apoptosis Induction with Enhancement of BAX/BCL2 Gene Expression Ratio via Combination Therapy in HT29 Colon Cancer Cells","authors":"Roya Bazzaz, P. Yaghmaei, S. Dastmalchi, N. Rashtchizadeh","doi":"10.22037/AMLS.V6.33489","DOIUrl":"https://doi.org/10.22037/AMLS.V6.33489","url":null,"abstract":"Background and Aim: Combination therapy is one of the new strategies that minimize resistance to chemotherapy and reduces drug toxicity. Here, we investigated the effect of combination therapy with 5-Fluorouracil and Gamma Tocopherol on cell survival and BAX/BCL2 gene expression ratio in HT29 colon cancer cells. \u0000Methods: The proliferation of cancer cells was determined via colony formation assay. \u0000BAX/BCL2 ratio was evaluated after incubation with concentrations of 5-Fluorouracil and Gamma Tocopherol via real-time-PCR. \u0000Results: The average number of colonies in the cells treated with 5-Fluorouracil, Gamma Tocopherol and their combination of them was 63±4, 78±3, and 28±2, respectively which significantly decreased in the combination group. In contrast with the control group, the BAX/BCL2 ratio remarkably increased when the cell underwent combinational treatment (p<0.05). \u0000Conclusion: 5-Fluorouracil and Gamma Tocopherol reduced HT 29 cell proliferation. Our results suggest that combination therapy with 5- Fluorouracil and Gamma Tocopherol can be considered as a strategy for induction of apoptosis via increasing the BAX/BCL2 ratio. \u0000*Corresponding Author: Nadereh Rashtchizadeh; Email: rashtchizadeh@rocketmail.com \u0000Please cite this article as: Bazzaz R, Yaghmaei P, Dastmalchi S, Rashtchizadeh N. Apoptosis Induction with Enhancement of BAX/BCL2 Gene Expression Ratio via Combination Therapy in HT29 Colon Cancer Cells. Arch Med Lab Sci. 2020;6:1-7 (e21). https://doi.org/10.22037/amls.v6.33489","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89062432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Aim: Inhalation of corticosteroids in patients with asthma is associated with an increased risk of osteoporosis. This study aimed to assess the effect of aerobic training on alkaline phosphatase (ALP), parathyroid hormone (PTH) as bone biomarkers in men with mild to moderate asthma. Methods: Twenty four sedentary middle-aged men with asthma aged 40±5 years were randomly assigned to two groups as exercise (aerobic training, n=12) and control (no training, n =12). Aerobic training was performed 3 days a week for 12 weeks in the form of running on a flat surface at 60-75% of HRmax. Fasting blood samples were taken before and after the training program for measuring serum ALP and PTH. Results: There were no statistically significant differences between groups with regard to anthropometrical and bone markers at baseline (p>0.05). Aerobic training resulted in significant increase in ALP (p = 0.023) and PTH (p = 0.016) in exercise individuals. All variables remained unchanged in the control group (p>0.05). Conclusion: With an emphasis on the increase in ALP and PTH, it is concluded that relatively long-term aerobic exercise is associated with improved bone formation markers in patients with asthma treated with inhaled corticosteroids. *Corresponding Author: Mojtaba Eizadi; Email: izadim@iau-saveh.ac.ir Please cite this article as: Eizadi M, Behboudi L, Afsharmand Z. The Effect of Aerobic Training on Parathyroid Hormone and Alkaline Phosphatase as Bone Markers in Men with Mild to Moderate Asthma. Arch Med Lab Sci. 2020;6:1-9 (e20). https://doi.org/10.22037/amls.v6. 33535
{"title":"The Effect of Aerobic Training on Parathyroid Hormone and Alkaline Phosphatase as Bone Markers in Men with Mild to Moderate Asthma","authors":"M. Eizadi, Laleh Behboudi, Z. Afsharmand","doi":"10.22037/AMLS.V6.33535","DOIUrl":"https://doi.org/10.22037/AMLS.V6.33535","url":null,"abstract":"Background and Aim: Inhalation of corticosteroids in patients with asthma is associated with an increased risk of osteoporosis. This study aimed to assess the effect of aerobic training on alkaline phosphatase (ALP), parathyroid hormone (PTH) as bone biomarkers in men with mild to moderate asthma. \u0000Methods: Twenty four sedentary middle-aged men with asthma aged 40±5 years were randomly assigned to two groups as exercise (aerobic training, n=12) and control (no training, n =12). Aerobic training was performed 3 days a week for 12 weeks in the form of running on a flat surface at 60-75% of HRmax. Fasting blood samples were taken before and after the training program for measuring serum ALP and PTH. \u0000Results: There were no statistically significant differences between groups with regard to anthropometrical and bone markers at baseline (p>0.05). Aerobic training resulted in significant increase in ALP (p = 0.023) and PTH (p = 0.016) in exercise individuals. All variables remained unchanged in the control group (p>0.05). \u0000Conclusion: With an emphasis on the increase in ALP and PTH, it is concluded that relatively long-term aerobic exercise is associated with improved bone formation markers in patients with asthma treated with inhaled corticosteroids. \u0000*Corresponding Author: Mojtaba Eizadi; Email: izadim@iau-saveh.ac.ir \u0000Please cite this article as: Eizadi M, Behboudi L, Afsharmand Z. The Effect of Aerobic Training on Parathyroid Hormone and Alkaline Phosphatase as Bone Markers in Men with Mild to Moderate Asthma. Arch Med Lab Sci. 2020;6:1-9 (e20). https://doi.org/10.22037/amls.v6. 33535","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81404763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ebrahimi, S. A. Aghapour, Susan Zamanpour Ziolaei
Background and Aim: Environmental factors play an important role in the development and progression of respiratory diseases. In this regard, the species Aspergillus and Candida fungi are very important environmental factors, which play a prominent role in the development, persistence, and severity of respiratory diseases, especially asthma. The study aimed to investigate the relationship between susceptibility to fungi and lung function in children referred to Deziani Allergy Clinic patients in 2010-2011. Methods: Children with age less than 12 years (n=40) were volunteered to participate in this case-control research. Two groups of patients susceptible to fungal (20/40) and not susceptible to fungal allergens (20/40) were selected. The groups were age and sex-matched. Demographic data and Forced Vital Capacity (FVC), Forced Expiratory Volume in the first second (FEV1) and Tiffeneau-Pinelli index was obtained from patient records. Statistical analysis performed by SPSS 16.0 program. Results: Examination of the lung function variables showed that the mean of FVC in the control group (76.075 liters/s) was higher than the patient group (71.5 liters/s). FEV1 was also higher in the control group than in the patient group (p<0.05). The Tiffeneau-Pinelli index was also higher in the control group than in the patient group (p<0.05). Conclusion: Allergy to fungi, such as Candida and Aspergillus, decreases lung function in children with asthma, which is directly related to reduce vital capacity in these patients. *Corresponding Author: Mohsen Ebrahimi; Email: m1355ebrahimi@gmail.com Please cite this article as: Ebrahimi M, Aghapour SA, Zamanpour Ziolaei S. The Relationship Between Decreased Lung Vital Capacity in Children with Respiratory Asthma Associated with Aspergillus and Candida in North of Iran. Arch Med Lab Sci. 2020;6:1-6 (e19). https://doi.org/10.22037/amls.v6.33170
背景与目的:环境因素在呼吸系统疾病的发生发展中起着重要作用。在这方面，曲霉和念珠菌真菌是非常重要的环境因素，它们在呼吸系统疾病，特别是哮喘的发生、持续和严重程度中起着突出的作用。本研究旨在探讨2010-2011年在Deziani过敏诊所就诊的儿童真菌易感性与肺功能的关系。方法:年龄小于12岁的儿童(n=40)自愿参加本病例对照研究。选择对真菌敏感(20/40)和对真菌过敏原不敏感(20/40)两组。这些小组的年龄和性别都是匹配的。统计资料及用力肺活量(FVC)、第一秒用力呼气量(FEV1)和Tiffeneau-Pinelli指数。采用SPSS 16.0软件进行统计分析。结果:肺功能变量检查显示，对照组FVC平均值(76.075 l /s)高于患者组(71.5 l /s)。对照组FEV1明显高于患者组(p<0.05)。对照组的Tiffeneau-Pinelli指数高于患者组(p<0.05)。结论:对念珠菌、曲霉菌等真菌过敏可导致哮喘患儿肺功能下降，这与肺活量降低有直接关系。*通讯作者:Mohsen Ebrahimi;Ebrahimi M, Aghapour SA, Zamanpour Ziolaei S.伊朗北部地区与曲霉和念珠菌相关的呼吸性哮喘患儿肺肺活量下降的关系中华医学杂志，2020;6:1-6 (e19)。https://doi.org/10.22037/amls.v6.33170
{"title":"The The Relationship between Decreased Lung Vital Capacity in Children with Respiratory Asthma Associated with Aspergillus and Candida in North of Iran","authors":"M. Ebrahimi, S. A. Aghapour, Susan Zamanpour Ziolaei","doi":"10.22037/AMLS.V6.33170","DOIUrl":"https://doi.org/10.22037/AMLS.V6.33170","url":null,"abstract":"Background and Aim: Environmental factors play an important role in the development and progression of respiratory diseases. In this regard, the species Aspergillus and Candida fungi are very important environmental factors, which play a prominent role in the development, persistence, and severity of respiratory diseases, especially asthma. The study aimed to investigate the relationship between susceptibility to fungi and lung function in children referred to Deziani Allergy Clinic patients in 2010-2011. \u0000Methods: Children with age less than 12 years (n=40) were volunteered to participate in this case-control research. Two groups of patients susceptible to fungal (20/40) and not susceptible to fungal allergens (20/40) were selected. The groups were age and sex-matched. Demographic data and Forced Vital Capacity (FVC), Forced Expiratory Volume in the first second (FEV1) and Tiffeneau-Pinelli index was obtained from patient records. Statistical analysis performed by SPSS 16.0 program. \u0000Results: Examination of the lung function variables showed that the mean of FVC in the control group (76.075 liters/s) was higher than the patient group (71.5 liters/s). FEV1 was also higher in the control group than in the patient group (p<0.05). The Tiffeneau-Pinelli index was also higher in the control group than in the patient group (p<0.05). \u0000Conclusion: Allergy to fungi, such as Candida and Aspergillus, decreases lung function in children with asthma, which is directly related to reduce vital capacity in these patients. \u0000*Corresponding Author: Mohsen Ebrahimi; Email: m1355ebrahimi@gmail.com \u0000Please cite this article as: Ebrahimi M, Aghapour SA, Zamanpour Ziolaei S. The Relationship Between Decreased Lung Vital Capacity in Children with Respiratory Asthma Associated with Aspergillus and Candida in North of Iran. Arch Med Lab Sci. 2020;6:1-6 (e19). https://doi.org/10.22037/amls.v6.33170","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78627931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Roya Vaziri Javid, Fahimeh Safizadeh, Mozhdeh Rastegary, Mohsen Nabi Afjadi, Abbas Khonakdar-Tarsi, Zahra Zare, S. Zarpour, Fariba Mohammadi Tahroodi
Background and Aim: Type 2 diabetes is a common metabolic disease, which is associated with obesity and inactivity. Plant-based dietary patterns are recommended for the treatment of type 2 diabetes. On the other hand, the effect of aerobic training on the improvement of type 2 diabetes is also highly emphasized. The purpose of this study was to investigate the effects of 2-months of pomegranate juice consumption with and without aerobic training on the levels of HbA1c, fasting blood glucose (FBG), lipid profile in women with type 2 diabetes. Methods: In this study, 58 women with type 2 diabetes who were referred to Tehran Lorzadeh clinic were randomly divided into four groups: control, pomegranate juice, aerobic training, combined pomegranate juice with aerobic training. The aerobic training plan consisted of 2-months and three times per week (training time from 20 minutes to 45 minutes). Three days before and after this interventional study, serum levels of FBG, total cholesterol (TC), triacylglyceride (TG), and low-density lipoprotein (LDL) were measured by colorimetric methods. Results: Results showed that combined pomegranate extract consumption with aerobic exercise training significantly decreased the levels of FBG, HbA1c, and TG in women with type 2 diabetes compared to the control group after the intervention (P<0.01), while TC, LDL-c, and BMI remained unchanged. There was no statistically significant difference in the levels of TC, TG, HbA1c, and FBG in pomegranate extract or aerobic training groups compared to the control group after intervention. Conclusion: The results of this study indicate that combined pomegranate juice with aerobic training has beneficial effects on type 2 diabetes and could be recommended in their therapeutic program. *Corresponding Author: Fariba Mohammadi Tahroodi; Email: faribamt93@yahoo.com Please cite this article as: Vaziri Javid R, Safizadeh F, Rastegary M, Nabi Afjadi M, Khonakdar-Tarsi A, Zare Z, Zarpou S, Mohammadi Tahroodi F. Effects of Pomegranate Juice with and without Aerobic Training on Glycemic Control and Lipid Profile in Women with Type 2 Diabetes. Arch Med Lab Sci. 2020;6.1-6 (e17). https://doi.org/10.22037/amls.v6.30381
背景与目的:2型糖尿病是一种常见的代谢性疾病，与肥胖和缺乏运动有关。植物性饮食模式被推荐用于治疗2型糖尿病。另一方面，有氧训练对改善2型糖尿病的作用也被高度重视。本研究的目的是研究2个月石榴汁饮用加有氧训练和不加有氧训练对2型糖尿病女性HbA1c、空腹血糖(FBG)、血脂水平的影响。方法:本研究将58例转诊至德黑兰Lorzadeh诊所的2型糖尿病女性患者随机分为4组:对照组、石榴汁组、有氧训练组、石榴汁加有氧训练组。有氧训练计划为2个月，每周3次(训练时间从20分钟到45分钟)。在介入研究前后3天，采用比色法测定血清FBG、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)水平。结果:结果表明，与对照组相比，干预后石榴提取物联合有氧运动训练显著降低了2型糖尿病女性的FBG、HbA1c、TG水平(P<0.01)，而TC、LDL-c、BMI保持不变。干预后，石榴提取物组和有氧训练组的TC、TG、HbA1c、FBG水平与对照组比较，差异无统计学意义。结论:石榴汁联合有氧训练对2型糖尿病患者有良好的治疗效果，可作为2型糖尿病患者的治疗方案。*通讯作者:Fariba Mohammadi Tahroodi;Vaziri Javid R, Safizadeh F, Rastegary M, Nabi Afjadi M, Khonakdar-Tarsi A, Zare Z, Zarpou S, Mohammadi Tahroodi F.有氧训练后石榴汁对2型糖尿病女性血糖控制和血脂的影响。中华医学杂志，2020;6.1-6 (e17)。https://doi.org/10.22037/amls.v6.30381
{"title":"Effects of Pomegranate Juice with and without Aerobic Training on Glycemic Control and Lipid Profile in Women with Type 2 Diabetes","authors":"Roya Vaziri Javid, Fahimeh Safizadeh, Mozhdeh Rastegary, Mohsen Nabi Afjadi, Abbas Khonakdar-Tarsi, Zahra Zare, S. Zarpour, Fariba Mohammadi Tahroodi","doi":"10.22037/AMLS.V6.30381","DOIUrl":"https://doi.org/10.22037/AMLS.V6.30381","url":null,"abstract":"Background and Aim: Type 2 diabetes is a common metabolic disease, which is associated with obesity and inactivity. Plant-based dietary patterns are recommended for the treatment of type 2 diabetes. On the other hand, the effect of aerobic training on the improvement of type 2 diabetes is also highly emphasized. The purpose of this study was to investigate the effects of 2-months of pomegranate juice consumption with and without aerobic training on the levels of HbA1c, fasting blood glucose (FBG), lipid profile in women with type 2 diabetes. \u0000Methods: In this study, 58 women with type 2 diabetes who were referred to Tehran Lorzadeh clinic were randomly divided into four groups: control, pomegranate juice, aerobic training, combined pomegranate juice with aerobic training. The aerobic training plan consisted of 2-months and three times per week (training time from 20 minutes to 45 minutes). Three days before and after this interventional study, serum levels of FBG, total cholesterol (TC), triacylglyceride (TG), and low-density lipoprotein (LDL) were measured by colorimetric methods. \u0000Results: Results showed that combined pomegranate extract consumption with aerobic exercise training significantly decreased the levels of FBG, HbA1c, and TG in women with type 2 diabetes compared to the control group after the intervention (P<0.01), while TC, LDL-c, and BMI remained unchanged. There was no statistically significant difference in the levels of TC, TG, HbA1c, and FBG in pomegranate extract or aerobic training groups compared to the control group after intervention. \u0000Conclusion: The results of this study indicate that combined pomegranate juice with aerobic training has beneficial effects on type 2 diabetes and could be recommended in their therapeutic program. \u0000*Corresponding Author: Fariba Mohammadi Tahroodi; Email: faribamt93@yahoo.com \u0000Please cite this article as: Vaziri Javid R, Safizadeh F, Rastegary M, Nabi Afjadi M, Khonakdar-Tarsi A, Zare Z, Zarpou S, Mohammadi Tahroodi F. Effects of Pomegranate Juice with and without Aerobic Training on Glycemic Control and Lipid Profile in Women with Type 2 Diabetes. Arch Med Lab Sci. 2020;6.1-6 (e17). https://doi.org/10.22037/amls.v6.30381","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83256297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Babak Sattartabar, Mahyar Nourian, Esmaeil Samizadeh, N. Mehrvar, H. Jalaeikhoo, A. Mehrvar, S. Iravani
Background and Aim: There are a limited number of studies regarding the clinicopathological features of gastric cancer. Here, the clinicopathological features of gastric cancer including tumor type and size, degree of differentiation, tumor operability, and immunohistochemically results of E-cadherin and P53 expression was investigated in Iranian patients. Methods: This retrospective study was performed on patients who were admitted at Imam Reza Hospital in Tehran-Iran from 2008 to 2017 with a diagnosis of gastric cancer. Required information including age, sex, type of cancer, pathology, and immunohistochemistry (IHC) results and treatment were extracted from the hospital archive. Results: Out of 264 enrolled patients (with the mean age of 70.03±14.01 years), 180 cases were men. The frequency of intestinal-type, Diffuse-type, lymphoma and GIST tumor were 54.5%, 27.7%, 12.25% and 5.92% respectively. In addition, 67.58% and 13.83% of the tumors were undifferentiated and poorly differentiated respectively. Surgery was less probable in less differentiated tumors (r=0.582 P=0.001). The surgery rate in GIST, intestinal-type, and Diffuse-type tumors were 100%, 57%, and 14.4%, respectively. IHC results showed that E-cadherin expression was present in 78.6, of which 50.9% were weakly positive (+1). Also, the high expression of P53 was observed in 60.7% of patients. Conclusion: According to the data, we can conclude that poorly differentiated tumors, decreased expression of E-cadherin, and increased expression of P53 is linked to poor prognosis in Iranian patients with gastric cancer. In this regard, further clinical trials and multicenter studies should be done to evaluate the possible factors for improving the prognosis and survival rates of Iranian patients with gastric cancer. *Corresponding Author: Shahrokh Iravani; Email: iravanishahrokh@ajaums.ac.ir Please cite this article as: Sattartabar B, Nourian M, Samizadeh E, Mehrvar N, Jalaeikhoo H, Mehrvar A, Iravani S. Clinicopathological Characteristics of Gastric Cancer in Iranian Patients Referred to Imam Reza Hospital During 2008 to 2017. Arch Med Lab Sci. 2020;6:1-6 (e18). https://doi.org/10.22037/amls.v6.33309
背景与目的:关于胃癌临床病理特征的研究数量有限。本文研究伊朗患者胃癌的临床病理特征，包括肿瘤的类型和大小、分化程度、肿瘤的可操作性以及E-cadherin和P53表达的免疫组织化学结果。方法:对2008年至2017年在伊朗德黑兰伊玛目礼萨医院(Imam Reza Hospital)确诊为胃癌的患者进行回顾性研究。所需信息包括年龄、性别、癌症类型、病理、免疫组化(IHC)结果和治疗，均从医院档案中提取。结果:264例入组患者(平均年龄70.03±14.01岁)，男性180例。肠型、弥漫性、淋巴瘤和GIST肿瘤的发生率分别为54.5%、27.7%、12.25%和5.92%。未分化率为67.58%，低分化率为13.83%。低分化肿瘤的手术可能性较小(r=0.582 P=0.001)。GIST型、肠型和弥漫性肿瘤的手术率分别为100%、57%和14.4%。免疫组化结果显示，78.6例患者E-cadherin表达，弱阳性占50.9%(+1)。60.7%的患者中P53高表达。结论:根据资料，我们可以得出伊朗胃癌患者肿瘤低分化、E-cadherin表达降低、P53表达升高与预后不良有关。因此，需要进一步的临床试验和多中心研究来评估改善伊朗胃癌患者预后和生存率的可能因素。*通讯作者:Shahrokh Iravani;Sattartabar B, Nourian M, Samizadeh E, Mehrvar N, Jalaeikhoo H, Mehrvar A, Iravani S.伊朗伊玛目雷扎医院2008 - 2017年转诊患者胃癌的临床病理特征中华医学杂志，2020;6:1-6 (e18)。https://doi.org/10.22037/amls.v6.33309
{"title":"Clinicopathological Characteristics of Gastric Cancer in Iranian Patients Referred to Imam Reza Hospital During 2008 to 2017","authors":"Babak Sattartabar, Mahyar Nourian, Esmaeil Samizadeh, N. Mehrvar, H. Jalaeikhoo, A. Mehrvar, S. Iravani","doi":"10.22037/AMLS.V6.33309","DOIUrl":"https://doi.org/10.22037/AMLS.V6.33309","url":null,"abstract":"Background and Aim: There are a limited number of studies regarding the clinicopathological features of gastric cancer. Here, the clinicopathological features of gastric cancer including tumor type and size, degree of differentiation, tumor operability, and immunohistochemically results of E-cadherin and P53 expression was investigated in Iranian patients. \u0000Methods: This retrospective study was performed on patients who were admitted at Imam Reza Hospital in Tehran-Iran from 2008 to 2017 with a diagnosis of gastric cancer. Required information including age, sex, type of cancer, pathology, and immunohistochemistry (IHC) results and treatment were extracted from the hospital archive. \u0000Results: Out of 264 enrolled patients (with the mean age of 70.03±14.01 years), 180 cases were men. The frequency of intestinal-type, Diffuse-type, lymphoma and GIST tumor were 54.5%, 27.7%, 12.25% and 5.92% respectively. In addition, 67.58% and 13.83% of the tumors were undifferentiated and poorly differentiated respectively. \u0000Surgery was less probable in less differentiated tumors (r=0.582 P=0.001). The surgery rate in GIST, intestinal-type, and Diffuse-type tumors were 100%, 57%, and 14.4%, respectively. IHC results showed that E-cadherin expression was present in 78.6, of which 50.9% were weakly positive (+1). Also, the high expression of P53 was observed in 60.7% of patients. \u0000Conclusion: According to the data, we can conclude that poorly differentiated tumors, decreased expression of E-cadherin, and increased expression of P53 is linked to poor prognosis in Iranian patients with gastric cancer. In this regard, further clinical trials and multicenter studies should be done to evaluate the possible factors for improving the prognosis and survival rates of Iranian patients with gastric cancer. \u0000*Corresponding Author: Shahrokh Iravani; Email: iravanishahrokh@ajaums.ac.ir \u0000Please cite this article as: Sattartabar B, Nourian M, Samizadeh E, Mehrvar N, Jalaeikhoo H, Mehrvar A, Iravani S. Clinicopathological Characteristics of Gastric Cancer in Iranian Patients Referred to Imam Reza Hospital During 2008 to 2017. Arch Med Lab Sci. 2020;6:1-6 (e18). https://doi.org/10.22037/amls.v6.33309","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80872336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Habib Hybar, A. Samimi, M. Maniati, Javad Jodat, Zahra Gatavizadeh
The prevalence of COVID-19 in the world is rapidly increasing. Although some patients show mild symptoms of the virus, some others need special care due to the exacerbation of the disease. Therefore, invasive treatments are needed to treat these patients. Data were collected from PubMed and Google scholars at various time points up to the 2020 academic year. The related keywords are listed as follows: "COVID-19", "Treatment", "Pathogenesis", and "Lung disorder". Studies have shown that although the use of ECMO and ventilation can provide oxygen to patients and improve their clinical status; these procedures can lead to the activation of inflammatory responses and the activation of the renin-angiotensin system. Inflammation and activation of the renin-angiotensin system are among the weak prognoses for COVID-19-infected patients. ECMO and ventilation treatment procedures are like double-edged swords, and monitoring patients during treatment is essential to prevent renin-angiotensin activation. *Corresponding Author: Zahra Gatavizadeh; Email: z-gatavizadeh@ajums.ac.ir Please cite this article as: Hybar H, Samimi A, Maniati M, Jodat J, Gatavizadeh Z. Evaluating Extracorporeal Membrane Oxygenation and Ventilation Treatment of Patients with COVID-19: A Review. Arch Med Lab Sci. 2020;6:1-6 (e16). https://doi.org/10.22037/amls.v6.33550
COVID-19在世界范围内的流行率正在迅速上升。虽然一些患者表现出轻微的病毒症状，但由于疾病的恶化，其他一些患者需要特别护理。因此，需要对这些患者进行侵入性治疗。数据是在2020学年之前的不同时间点从PubMed和谷歌学者那里收集的。相关关键词如下:“COVID-19”、“治疗”、“发病机制”、“肺部疾病”。研究表明，虽然使用ECMO和通气可以为患者提供氧气，改善其临床状况;这些过程可导致炎症反应的激活和肾素-血管紧张素系统的激活。感染covid -19的患者预后不佳的因素包括炎症和肾素-血管紧张素系统的激活。ECMO和通气治疗过程就像一把双刃剑，在治疗过程中对患者进行监测对于防止肾素-血管紧张素激活至关重要。*通讯作者:Zahra Gatavizadeh;Hybar H, Samimi A, Maniati M, Jodat J, Gatavizadeh Z.体外膜氧合和通气治疗新冠肺炎的研究进展中华医学杂志，2020;6:1-6 (e16)。https://doi.org/10.22037/amls.v6.33550
{"title":"Evaluating Extracorporeal Membrane Oxygenation and Ventilation Treatment of Patients with COVID-19: A Review","authors":"Habib Hybar, A. Samimi, M. Maniati, Javad Jodat, Zahra Gatavizadeh","doi":"10.22037/AMLS.V6.33550","DOIUrl":"https://doi.org/10.22037/AMLS.V6.33550","url":null,"abstract":"The prevalence of COVID-19 in the world is rapidly increasing. Although some patients show mild symptoms of the virus, some others need special care due to the exacerbation of the disease. Therefore, invasive treatments are needed to treat these patients. Data were collected from PubMed and Google scholars at various time points up to the 2020 academic year. The related keywords are listed as follows: \"COVID-19\", \"Treatment\", \"Pathogenesis\", and \"Lung disorder\". Studies have shown that although the use of ECMO and ventilation can provide oxygen to patients and improve their clinical status; these procedures can lead to the activation of inflammatory responses and the activation of the renin-angiotensin system. Inflammation and activation of the renin-angiotensin system are among the weak prognoses for COVID-19-infected patients. ECMO and ventilation treatment procedures are like double-edged swords, and monitoring patients during treatment is essential to prevent renin-angiotensin activation. \u0000*Corresponding Author: Zahra Gatavizadeh; Email: z-gatavizadeh@ajums.ac.ir \u0000Please cite this article as: Hybar H, Samimi A, Maniati M, Jodat J, Gatavizadeh Z. Evaluating Extracorporeal Membrane Oxygenation and Ventilation Treatment of Patients with COVID-19: A Review. Arch Med Lab Sci. 2020;6:1-6 (e16). https://doi.org/10.22037/amls.v6.33550","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73705569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Babaei, F. A. Abi, Susan Rostampur, Iman Pouladi, Faranak Rezaei
Background and Aim: Brucellosis (also known as undulant fever, Mediterranean fever, or Malta fever) is a zoonotic infection transmitted to humans from infected animals (sheep, cattle, goats, pigs, camels, or other animals) by ingestion of food products (such as unpasteurized dairy products) or by contact with tissue or fluids. This disease occurs on average in 11 to 56 percent of children in endemic regions. Despite being long recognized and controllable, the disease still causes substantial morbidity, affecting especially the young population. The objective of this research was to examine the clinical, laboratory results of childhood brucellosis and brucellosis epidemiological features in patients up to 15 years in the province in Pol-e Dokhtar city, Iran. Methods The diagnosis of brucellosis was established on the basis of clinical features, and positive results from Serologic evaluation included Wright test and the 2-mercaptoethanol (2ME) test. This information includes medical records of brucellosis patients in the years 2016 to 2019. SPSS software (version 22.0) was used for the data analysis. Results: Among the patients infected with Brucella, 19 males (70.37%) and 8 females (29.63%) were diagnosed and 11 patients (40.74%) were 5-9 years of age, 10 patients (37%) were 10-15 years of age, and 6 patients (22.26%) were < 5 year of age. Also 27 (100%) patients were from rural regions (the total of affected children) that confirmed direct contact with infected animals in all of them was already established. The most frequent seasons of disease occurrence were summer (33.38%) and winter (29.6%). Conclusion: Considering that 100% of the infection of children has been seen in those who lived in the village, it requires regular vaccination of livestock and proper training in the field of disease prevention. *Corresponding Authors: Faranak Rezaei, Email: Rezaei.f@lums.ac.ir; Iman Pouladi, Email: imanpouladi96a@gmail.com Please cite this article as: Babaei Z, Azizi abi F, Pouladi I, Rezaei F. Investigation of Pediatric Brucellosis in Pol-e Dokhtar city, Iran. Arch Med Lab Sci. 2020;6:1-4 (e14). https://doi.org/10.22037/amls.v6.30546
{"title":"Investigation of Pediatric Brucellosis","authors":"Z. Babaei, F. A. Abi, Susan Rostampur, Iman Pouladi, Faranak Rezaei","doi":"10.22037/AMLS.V6.30546","DOIUrl":"https://doi.org/10.22037/AMLS.V6.30546","url":null,"abstract":"Background and Aim: Brucellosis (also known as undulant fever, Mediterranean fever, or Malta fever) is a zoonotic infection transmitted to humans from infected animals (sheep, cattle, goats, pigs, camels, or other animals) by ingestion of food products (such as unpasteurized dairy products) or by contact with tissue or fluids. This disease occurs on average in 11 to 56 percent of children in endemic regions. Despite being long recognized and controllable, the disease still causes substantial morbidity, affecting especially the young population. The objective of this research was to examine the clinical, laboratory results of childhood brucellosis and brucellosis epidemiological features in patients up to 15 years in the province in Pol-e Dokhtar city, Iran. \u0000Methods The diagnosis of brucellosis was established on the basis of clinical features, and positive results from Serologic evaluation included Wright test and the 2-mercaptoethanol (2ME) test. This information includes medical records of brucellosis patients in the years 2016 to 2019. SPSS software (version 22.0) was used for the data analysis. \u0000Results: Among the patients infected with Brucella, 19 males (70.37%) and 8 females (29.63%) were diagnosed and 11 patients (40.74%) were 5-9 years of age, 10 patients (37%) were 10-15 years of age, and 6 patients (22.26%) were < 5 year of age. Also 27 (100%) patients were from rural regions (the total of affected children) that confirmed direct contact with infected animals in all of them was already established. The most frequent seasons of disease occurrence were summer (33.38%) and winter (29.6%). \u0000Conclusion: Considering that 100% of the infection of children has been seen in those who lived in the village, it requires regular vaccination of livestock and proper training in the field of disease prevention. \u0000*Corresponding Authors: Faranak Rezaei, Email: Rezaei.f@lums.ac.ir; Iman Pouladi, Email: imanpouladi96a@gmail.com \u0000Please cite this article as: Babaei Z, Azizi abi F, Pouladi I, Rezaei F. Investigation of Pediatric Brucellosis in Pol-e Dokhtar city, Iran. Arch Med Lab Sci. 2020;6:1-4 (e14). https://doi.org/10.22037/amls.v6.30546","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85745714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Aim: Urinary tract infections (UTIs) are one of the most common infectious diseases. Although UTI is mostly associated with several members of the family of Enterobacteriaceae, Escherichia coli (E. coli) is the most common pathogen among them. This study aims to determine the prevalence and resistance pattern of ESBL producing E. coli isolated from patients with urinary tract infection in Sari, Iran. Methods: From December-2016 to June-2017, a hospital-based cross-sectional work was accompanied, and a total of 200 urine samples were cultured on blood agar and MacConkey agar for the identification of etiologic agents. After detection and confirmation of E. coli isolates, susceptibility testing was assessed using the following antibiotics: cefotaxime, ceftazidime, imipenem, nalidixic acid, cefixime, amikacin, ofloxacin, ceftriaxone, cefepime, gentamicin, tobramycin, meropenem, piracetam, and ciprofloxacin with Kirby–Bauer disk-diffusion technique according to the CLSI guidelines. Double-disk synergy (DDS) methods were used for the detection of ESBL-producing strains. Results: In the current study, 120 urinary isolates of E. coli were detected, which ESBL-producing phenotypes were detected in 55% (n = 66) of the isolates. ESBL producing strains of E. coli showed the highest susceptibility to meropenem (100%) and ofloxacin (96%); and showed the highest rates of resistance to ceftazidime (91%), cefepime (87%), cefotaxime, and ceftriaxone (84%). Conclusion: Markedly high resistance to third-generation cephalosporins among E. coli strains was found in the current study. Considering the high prevalence of resistance to third-generation cephalosporins in infections caused by organisms producing ESBL, performing comprehensive tests before prescribing antibiotics is essential for the management of infections caused by these strains in community/hospital-acquired UTIs. Furthermore accompanying molecular-based works on ESBL variants will assistance to achieve better results. *Corresponding Author: Mehrdad Gholami; Email: me.gholami@mazums.ac.ir Please cite this article as: Ahanjan M, Salehian M, Gholami M. Prevalence and Resistance Pattern of Extended-Spectrum β-Lactamase Producing Escherichia coli Isolated from Patients with Urinary Tract Infection. Arch Med Lab Sci. 2020;6:1-7 (e13). https://doi.org/10.22037/amls.v6.33081
背景与目的:尿路感染是最常见的感染性疾病之一。虽然UTI主要与肠杆菌科的几个成员有关，但大肠杆菌(E. coli)是其中最常见的病原体。本研究旨在确定从伊朗萨里市尿路感染患者中分离出的产生ESBL的大肠杆菌的流行情况和耐药模式。方法:2016年12月至2017年6月，以医院为单位开展横断面工作，在血琼脂和麦康基琼脂上培养200份尿样，进行病原鉴定。检测并确认大肠杆菌分离株后，采用kir比- bauer盘片扩散技术，采用头孢噻肟、头孢他啶、亚胺培南、萘啶酸、头孢克肟、阿米卡星、氧氟沙星、头孢曲松、头孢吡肟、庆大霉素、妥布霉素、美罗培南、吡拉西坦、环丙沙星等抗生素进行药敏试验。采用双盘协同法(DDS)检测产esbl菌株。结果:本研究共检出120株尿路大肠杆菌，其中55% (n = 66)的分离株检出产esbl表型。产ESBL的大肠杆菌对美罗培南(100%)和氧氟沙星(96%)的敏感性最高;对头孢他啶(91%)、头孢吡肟(87%)、头孢噻肟和头孢曲松(84%)的耐药率最高。结论:本研究发现大肠杆菌对第三代头孢菌素具有明显的高耐药性。考虑到在产生ESBL的生物体引起的感染中对第三代头孢菌素的耐药性很高，在开抗生素处方之前进行全面测试对于管理社区/医院获得性尿路感染中这些菌株引起的感染至关重要。此外，伴随的基于分子的ESBL变体工作将有助于取得更好的结果。*通讯作者:Mehrdad Gholami;Ahanjan M, Salehian M, Gholami M.尿路感染患者产β-内酰胺酶大肠埃希菌的流行及耐药模式中华医学杂志，2020;6:1-7 (e13)。https://doi.org/10.22037/amls.v6.33081
{"title":"Prevalence and Resistance Pattern of Extended-Spectrum β-Lactamase Producing Escherichia coli Isolated from Patients with Urinary Tract Infection","authors":"M. Ahanjan, M. Salehian, M. Gholami","doi":"10.22037/AMLS.V6.33081","DOIUrl":"https://doi.org/10.22037/AMLS.V6.33081","url":null,"abstract":"Background and Aim: Urinary tract infections (UTIs) are one of the most common infectious diseases. Although UTI is mostly associated with several members of the family of Enterobacteriaceae, Escherichia coli (E. coli) is the most common pathogen among them. This study aims to determine the prevalence and resistance pattern of ESBL producing E. coli isolated from patients with urinary tract infection in Sari, Iran. \u0000Methods: From December-2016 to June-2017, a hospital-based cross-sectional work was accompanied, and a total of 200 urine samples were cultured on blood agar and MacConkey agar for the identification of etiologic agents. After detection and confirmation of E. coli isolates, susceptibility testing was assessed using the following antibiotics: cefotaxime, ceftazidime, imipenem, nalidixic acid, cefixime, amikacin, ofloxacin, ceftriaxone, cefepime, gentamicin, tobramycin, meropenem, piracetam, and ciprofloxacin with Kirby–Bauer disk-diffusion technique according to the CLSI guidelines. Double-disk synergy (DDS) methods were used for the detection of ESBL-producing strains. \u0000Results: In the current study, 120 urinary isolates of E. coli were detected, which ESBL-producing phenotypes were detected in 55% (n = 66) of the isolates. ESBL producing strains of E. coli showed the highest susceptibility to meropenem (100%) and ofloxacin (96%); and showed the highest rates of resistance to ceftazidime (91%), cefepime (87%), cefotaxime, and ceftriaxone (84%). \u0000Conclusion: Markedly high resistance to third-generation cephalosporins among E. coli strains was found in the current study. Considering the high prevalence of resistance to third-generation cephalosporins in infections caused by organisms producing ESBL, performing comprehensive tests before prescribing antibiotics is essential for the management of infections caused by these strains in community/hospital-acquired UTIs. Furthermore accompanying molecular-based works on ESBL variants will assistance to achieve better results. \u0000*Corresponding Author: Mehrdad Gholami; Email: me.gholami@mazums.ac.ir \u0000Please cite this article as: Ahanjan M, Salehian M, Gholami M. Prevalence and Resistance Pattern of Extended-Spectrum β-Lactamase Producing Escherichia coli Isolated from Patients with Urinary Tract Infection. Arch Med Lab Sci. 2020;6:1-7 (e13). https://doi.org/10.22037/amls.v6.33081","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90153814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Esmailidehaj, S. Mirhosseini, Zakieh Sadat Sheikhalishahi
Background and Aim: Left ventricular dysfunction is a frequent complication after coronary artery bypass graft (CABG) and is often treated with inotropic drugs to achieve suitable hemodynamic status. Bone marrow-derived mesenchymal stem cells (BMSCs) have potential effects on cardiac function. In this study, we aimed to identify the predictor role of MSCs in taking inotropic drugs in patients with severe left ventricular dysfunction undergoing CABG. Methods: The study included 30 patients who underwent off-pump CABG at Afshar Hospital and Seyed Al-Shohada Hospital. For investigating the possible association of BMSCs proliferation rate with taking inotropic drugs, the bone marrow samples aspired from patients' sternum during surgery. MSCs were isolated and counted after 4, 7, and 14 days using trypan-blue color, and then doubling times were calculated. Results: After cardiac surgery, the number of female patients who take inotropic drugs was significantly higher than men. Our data showed that the BMSCs doubling time in female patients who received inotropic drugs was less than that of male patients who received inotropic drugs (p < 0.05). Conclusion: Based on this investigation, we concluded that there was a clear relevance between the MSCs' doubling time and the inotropic drug requirements in patients who received inotropic drugs. *Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: z.sheikhalishahi@gmail.com Please cite this article as Esmailidehaj M, Mirhosseini S.J, Sheikhalishahi Z.S. Association Between Bone Marrow Mesenchymal Stem Cell Characterization and Taking Inotropic Drugs in Patients with Severe Left Ventricular Dysfunction After Off-pump Bypass Surgery. Arch Med Lab Sci. 2020;6:1-6 (e14). https://doi.org/10.22037/amls.v6.31298
{"title":"Association between Bone Marrow Mesenchymal Stem Cell Characterization and Taking Inotropic Drugs in Patients with Severe Left Ventricular Dysfunction After Off-pump Bypass Surgery","authors":"M. Esmailidehaj, S. Mirhosseini, Zakieh Sadat Sheikhalishahi","doi":"10.22037/AMLS.V6.31298","DOIUrl":"https://doi.org/10.22037/AMLS.V6.31298","url":null,"abstract":"Background and Aim: Left ventricular dysfunction is a frequent complication after coronary artery bypass graft (CABG) and is often treated with inotropic drugs to achieve suitable hemodynamic status. Bone marrow-derived mesenchymal stem cells (BMSCs) have potential effects on cardiac function. In this study, we aimed to identify the predictor role of MSCs in taking inotropic drugs in patients with severe left ventricular dysfunction undergoing CABG. \u0000Methods: The study included 30 patients who underwent off-pump CABG at Afshar Hospital and Seyed Al-Shohada Hospital. For investigating the possible association of BMSCs proliferation rate with taking inotropic drugs, the bone marrow samples aspired from patients' sternum during surgery. MSCs were isolated and counted after 4, 7, and 14 days using trypan-blue color, and then doubling times were calculated. \u0000Results: After cardiac surgery, the number of female patients who take inotropic drugs was significantly higher than men. Our data showed that the BMSCs doubling time in female patients who received inotropic drugs was less than that of male patients who received inotropic drugs (p < 0.05). \u0000Conclusion: Based on this investigation, we concluded that there was a clear relevance between the MSCs' doubling time and the inotropic drug requirements in patients who received inotropic drugs. \u0000*Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: z.sheikhalishahi@gmail.com \u0000Please cite this article as Esmailidehaj M, Mirhosseini S.J, Sheikhalishahi Z.S. Association Between Bone Marrow Mesenchymal Stem Cell Characterization and Taking Inotropic Drugs in Patients with Severe Left Ventricular Dysfunction After Off-pump Bypass Surgery. Arch Med Lab Sci. 2020;6:1-6 (e14). https://doi.org/10.22037/amls.v6.31298","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86353573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Shojaee, P. Mohammadi, H. Jafarpour, Somayeh Pouriamehr, Haleh Barmaki, Abbas Khonakdar-Tarsi
Background and Aim: Prostate cancer (PC) is one of the most prevalent cancers with high mortality and morbidity in men, which can be treated in different ways before the progression and metastasis to distant organs. Destruction of extracellular matrix by matrix metalloproteinase (MMP), particularly by the 2 and 9 subtypes, has an important role in the metastasis of PC. We aimed to assess the activity of MMP 2 and 9 and some related metalloproteinases in PC and with benign prostate hyperplasia (BPH) patients in comparison to healthy individuals. Methods: In this case-control study, 72 individuals referred to Imam Khomeini hospital (Tehran, Iran), have been divided into 3 groups, including PC, BPH, and healthy control. Age and body mass index (BMI) for all groups have been matched. Venous blood samples were used to assess the enzyme activity by the zymography technique. Results: The activity of MMP-2 and 9 was significantly higher in PC than BPH and control groups. But there was no difference in the activity of enzymes in patients with PC according to the Gleason score. Conclusion: The results suggested that MMPs activity can be considered a diagnostic marker for PC. However, further studies are required to establish this concept. *Corresponding Author: Abbas Khonakdar-Tarsi; Email: a.khonakdar@mazums.ac.ir Please cite this article as: Shojaee M, Mohammadi P, Jafarpour H, Pouriamehr S, Barmaki H, Khonakdar-Tarsi A. Evaluation of Matrix Metalloproteinase 2 and 9 Activity in Patients with Prostate Cancer and Benign Prostate Hyperplasia Compared with Healthy Individuals. Arch Med Lab Sci. 2020;6:1-6(e12). https://doi.org/10.22037/amls.v6.32378
{"title":"Evaluation of Matrix Metalloproteinase 2 and 9 Activity in Patients with Prostate Cancer and Benign Prostate Hyperplasia Compared with Healthy Individuals","authors":"M. Shojaee, P. Mohammadi, H. Jafarpour, Somayeh Pouriamehr, Haleh Barmaki, Abbas Khonakdar-Tarsi","doi":"10.22037/AMLS.V6.32378","DOIUrl":"https://doi.org/10.22037/AMLS.V6.32378","url":null,"abstract":"Background and Aim: Prostate cancer (PC) is one of the most prevalent cancers with high mortality and morbidity in men, which can be treated in different ways before the progression and metastasis to distant organs. Destruction of extracellular matrix by matrix metalloproteinase (MMP), particularly by the 2 and 9 subtypes, has an important role in the metastasis of PC. We aimed to assess the activity of MMP 2 and 9 and some related metalloproteinases in PC and with benign prostate hyperplasia (BPH) patients in comparison to healthy individuals. \u0000Methods: In this case-control study, 72 individuals referred to Imam Khomeini hospital (Tehran, Iran), have been divided into 3 groups, including PC, BPH, and healthy control. Age and body mass index (BMI) for all groups have been matched. Venous blood samples were used to assess the enzyme activity by the zymography technique. \u0000Results: The activity of MMP-2 and 9 was significantly higher in PC than BPH and control groups. But there was no difference in the activity of enzymes in patients with PC according to the Gleason score. \u0000Conclusion: The results suggested that MMPs activity can be considered a diagnostic marker for PC. However, further studies are required to establish this concept. \u0000*Corresponding Author: Abbas Khonakdar-Tarsi; Email: a.khonakdar@mazums.ac.ir \u0000Please cite this article as: Shojaee M, Mohammadi P, Jafarpour H, Pouriamehr S, Barmaki H, Khonakdar-Tarsi A. Evaluation of Matrix Metalloproteinase 2 and 9 Activity in Patients with Prostate Cancer and Benign Prostate Hyperplasia Compared with Healthy Individuals. Arch Med Lab Sci. 2020;6:1-6(e12). https://doi.org/10.22037/amls.v6.32378","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84370881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：