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Various microbial taxa couple arsenic transformation to nitrogen and carbon cycling in paddy soils. 各种微生物类群将砷转化与稻田土壤中的氮和碳循环联系起来。
IF 13.8 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-14 DOI: 10.1186/s40168-024-01952-4
Xin-Di Zhao, Zi-Yu Gao, Jingjing Peng, Konstantinos T Konstantinidis, Si-Yu Zhang

Background: Arsenic (As) metabolism pathways and their coupling to nitrogen (N) and carbon (C) cycling contribute to elemental biogeochemical cycling. However, how whole-microbial communities respond to As stress and which taxa are the predominant As-transforming bacteria or archaea in situ remains unclear. Hence, by constructing and applying ROCker profiles to precisely detect and quantify As oxidation (aioA, arxA) and reduction (arrA, arsC1, arsC2) genes in short-read metagenomic and metatranscriptomic datasets, we investigated the dominant microbial communities involved in arsenite (As(III)) oxidation and arsenate (As(V)) reduction and revealed their potential pathways for coupling As with N and C in situ in rice paddies.

Results: Five ROCker models were constructed to quantify the abundance and transcriptional activity of short-read sequences encoding As oxidation (aioA and arxA) and reduction (arrA, arsC1, arsC2) genes in paddy soils. Our results revealed that the sub-communities carrying the aioA and arsC2 genes were predominantly responsible for As(III) oxidation and As(V) reduction, respectively. Moreover, a newly identified As(III) oxidation gene, arxA, was detected in genomes assigned to various phyla and showed significantly increased transcriptional activity with increasing soil pH, indicating its important role in As(III) oxidation in alkaline soils. The significant correlation of the transcriptional activities of aioA with the narG and nirK denitrification genes, of arxA with the napA and nirS denitrification genes and of arrA/arsC2 with the pmoA and mcrA genes implied the coupling of As(III) oxidation with denitrification and As(V) reduction with methane oxidation. Various microbial taxa including Burkholderiales, Desulfatiglandales, and Hyphomicrobiales (formerly Rhizobiales) are involved in the coupling of As with N and C metabolism processes. Moreover, these correlated As and N/C genes often co-occur in the same genome and exhibit greater transcriptional activity in paddy soils with As contamination than in those without contamination.

Conclusions: Our results revealed the comprehensive detection and typing of short-read sequences associated with As oxidation and reduction genes via custom-built ROCker models, and shed light on the various microbial taxa involved in the coupling of As and N and C metabolism in situ in paddy soils. The contribution of the arxA sub-communities to the coupling of As(III) oxidation with nitrate reduction and the arsC sub-communities to the coupling of As(V) reduction with methane oxidation expands our knowledge of the interrelationships among As, N, and C cycling in paddy soils. Video Abstract.

背景:砷(As)代谢途径及其与氮(N)和碳(C)循环的耦合有助于元素生物地球化学循环。然而,整个微生物群落如何应对砷胁迫以及哪些类群是原位砷转化的主要细菌或古细菌仍不清楚。因此,通过构建和应用 ROCker 图谱来精确检测和量化短读数元基因组和元转录组数据集中的砷氧化(aioA、arxA)和砷还原(arrA、arsC1、arsC2)基因,我们研究了参与亚砷酸盐(As(III))氧化和砷酸盐(As(V))还原的主要微生物群落,并揭示了它们在稻田中将砷与氮和碳原位耦合的潜在途径:结果:我们构建了五个 ROCker 模型,以量化水稻田土壤中编码砷氧化(aioA 和 arxA)和砷还原(arrA、arsC1 和 arsC2)基因的短读序列的丰度和转录活性。我们的研究结果表明,携带 aioA 和 arsC2 基因的亚群落分别主要负责 As(III) 氧化和 As(V) 还原。此外,在不同门类的基因组中发现了一个新鉴定的 As(III)氧化基因 arxA,其转录活性随着土壤 pH 值的增加而显著增加,表明它在碱性土壤中的 As(III)氧化过程中发挥着重要作用。aioA 的转录活性与 narG 和 nirK 反硝化基因、arxA 的转录活性与 napA 和 nirS 反硝化基因、arrA/arsC2 的转录活性与 pmoA 和 mcrA 基因之间存在明显的相关性,这意味着 As(III)氧化与反硝化以及 As(V)还原与甲烷氧化之间存在耦合关系。包括 Burkholderiales、Desulfatiglandales 和 Hyphomicrobiales(前根瘤菌)在内的各种微生物类群都参与了 As 与氮和碳代谢过程的耦合。此外,这些与砷和氮/碳相关的基因往往同时出现在同一基因组中,并且在受砷污染的稻田土壤中比在未受砷污染的土壤中表现出更强的转录活性:我们的研究结果揭示了通过定制的 ROCker 模型对与砷氧化和还原基因相关的短读序列的全面检测和分型,并揭示了参与水稻田土壤中砷与氮和碳原位代谢耦合的各种微生物类群。arxA 亚群落对 As(III) 氧化与硝酸盐还原耦合的贡献以及 arsC 亚群落对 As(V) 还原与甲烷氧化耦合的贡献扩展了我们对水稻田土壤中 As、N 和 C 循环之间相互关系的认识。视频摘要。
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引用次数: 0
An integrated microbiome- and metabolome-genome-wide association study reveals the role of heritable ruminal microbial carbohydrate metabolism in lactation performance in Holstein dairy cows. 一项综合微生物组和代谢组全基因组关联研究揭示了荷斯坦奶牛瘤胃微生物碳水化合物代谢在泌乳性能中的遗传作用。
IF 13.8 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-12 DOI: 10.1186/s40168-024-01937-3
Chenguang Zhang, Huifeng Liu, Xingwei Jiang, Zhihong Zhang, Xinfeng Hou, Yue Wang, Dangdang Wang, Zongjun Li, Yangchun Cao, Shengru Wu, Sharon A Huws, Junhu Yao
<p><strong>Background: </strong>Despite the growing number of studies investigating the connection between host genetics and the rumen microbiota, there remains a dearth of systematic research exploring the composition, function, and metabolic traits of highly heritable rumen microbiota influenced by host genetics. Furthermore, the impact of these highly heritable subsets on lactation performance in cows remains unknown. To address this gap, we collected and analyzed whole-genome resequencing data, rumen metagenomes, rumen metabolomes and short-chain fatty acids (SCFAs) content, and lactation performance phenotypes from a cohort of 304 dairy cows.</p><p><strong>Results: </strong>The results indicated that the proportions of highly heritable subsets (h<sup>2</sup> ≥ 0.2) of the rumen microbial composition (55%), function (39% KEGG and 28% CAZy), and metabolites (18%) decreased sequentially. Moreover, the highly heritable microbes can increase energy-corrected milk (ECM) production by reducing the rumen acetate/propionate ratio, according to the structural equation model (SEM) analysis (CFI = 0.898). Furthermore, the highly heritable enzymes involved in the SCFA synthesis metabolic pathway can promote the synthesis of propionate and inhibit the acetate synthesis. Next, the same significant SNP variants were used to integrate information from genome-wide association studies (GWASs), microbiome-GWASs, metabolome-GWASs, and microbiome-wide association studies (mWASs). The identified single nucleotide polymorphisms (SNPs) of rs43470227 and rs43472732 on SLC30A9 (Zn<sup>2+</sup> transport) (P < 0.05/nSNPs) can affect the abundance of rumen microbes such as Prevotella_sp., Prevotella_sp._E15-22, Prevotella_sp._E13-27, which have the oligosaccharide-degradation enzymes genes, including the GH10, GH13, GH43, GH95, and GH115 families. The identified SNPs of chr25:11,177 on 5s_rRNA (small ribosomal RNA) (P < 0.05/nSNPs) were linked to ECM, the abundance alteration of Pseudobutyrivibrio_sp. (a genus that was also showed to be linked to the ECM production via the mWASs analysis), GH24 (lysozyme), and 9,10,13-TriHOME (linoleic acid metabolism). Moreover, ECM, and the abundances of Pseudobutyrivibrio sp., GH24, and 9,10,13-TRIHOME were significantly greater in the GG genotype than in the AG genotype at chr25:11,177 (P < 0.05). By further the SEM analysis, GH24 was positively correlated with Pseudobutyrivibrio sp., which was positively correlated with 9,10,13-triHOME and subsequently positively correlated with ECM (CFI = 0.942).</p><p><strong>Conclusion: </strong>Our comprehensive study revealed the distinct heritability patterns of rumen microbial composition, function, and metabolism. Additionally, we shed light on the influence of host SNP variants on the rumen microbes with carbohydrate metabolism and their subsequent effects on lactation performance. Collectively, these findings offer compelling evidence for the host-microbe interactions, wherein cows active
背景:尽管越来越多的研究调查了宿主遗传与瘤胃微生物群之间的联系,但仍缺乏系统的研究来探讨受宿主遗传影响的高遗传性瘤胃微生物群的组成、功能和代谢特征。此外,这些高遗传亚群对奶牛泌乳性能的影响仍然未知。为了填补这一空白,我们收集并分析了304头奶牛的全基因组重测序数据、瘤胃元基因组、瘤胃代谢组和短链脂肪酸(SCFAs)含量以及泌乳性能表型:结果表明,瘤胃微生物组成(55%)、功能(39% KEGG 和 28% CAZy)和代谢物(18%)的高遗传亚群比例(h2 ≥ 0.2)依次下降。此外,根据结构方程模型(SEM)分析(CFI = 0.898),高遗传性微生物可通过降低瘤胃乙酸盐/丙酸盐比率来提高能量校正奶(ECM)产量。此外,参与 SCFA 合成代谢途径的高遗传性酶可促进丙酸盐的合成,抑制乙酸盐的合成。接下来,我们利用相同的重要 SNP 变异整合了全基因组关联研究(GWAS)、微生物组-GWAS、代谢组-GWAS 和全微生物组关联研究(mWAS)的信息。在 SLC30A9(Zn2+转运)上发现了 rs43470227 和 rs43472732 的单核苷酸多态性(SNPs)(P 结论):我们的综合研究揭示了瘤胃微生物组成、功能和代谢的独特遗传性模式。此外,我们还揭示了宿主 SNP 变异对瘤胃微生物与碳水化合物代谢的影响及其对泌乳性能的后续影响。总之,这些发现为宿主与微生物之间的相互作用提供了令人信服的证据,即奶牛通过SNP变异主动调节其瘤胃微生物群,从而调节自身的泌乳性能。视频摘要。
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引用次数: 0
Melatonin alleviates heat stress-induced spermatogenesis dysfunction in male dairy goats by regulating arachidonic acid metabolism mediated by remodeling the gut microbiota. 褪黑素通过重塑肠道微生物群来调节花生四烯酸代谢,从而缓解热应激诱发的雄性奶山羊精子发生功能障碍。
IF 13.8 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-12 DOI: 10.1186/s40168-024-01942-6
Xinrui Guo, Jing Xu, Yongkang Zhao, Jie Wang, Tingshu Fu, Mathias L Richard, Harry Sokol, Miao Wang, Yu Li, Yang Liu, Hui Wang, Chenlei Wang, Xueqin Wang, Haiyang He, Yazhou Wang, Baohua Ma, Sha Peng

Background: Heat stress (HS) commonly occurring in summer has gradually become a factor threatening the reproductive performance of male dairy goats by reducing their fecundity. Despite the melatonin is applied to relieve HS, it is still unclear whether melatonin protects against reproductive damage induced by HS in dairy goats and how it works. The purpose of the present study is to evaluate the role of melatonin in alleviating HS-induced spermatogenesis dysfunction in male dairy goats and further explore its mechanism.

Results: HS impaired spermatogenesis, sperm formation in the testes, and sperm maturation in the epididymis of dairy goats, resulting in decreased sperm quality. Melatonin rescued the decrease of sperm quality induced by HS via decreasing inflammatory and oxidative stress levels in testicular tissue and enhancing intercellular barrier function within the testes. Amplicon-based microbiota analysis revealed that despite gut microbiota differences between melatonin-treated dairy goats and NC dairy goats to some extent, melatonin administration tends to return the gut microbiota of male dairy goats under HS to the levels of natural control dairy goats. To explore whether the protective role of melatonin in sperm quality is mediated by regulating gut microbiota, fecal microbiota of HS dairy goats with or without melatonin treatment were transferred to HS mice, respectively. We found HS mice that had received fecal bacteria of HS dairy goats experienced serious testicular injury and dyszoospermia, while this phenomenon was ameliorated in HS mice that had received fecal bacteria of dairy goats treated with melatonin, indicating melatonin alleviates HS-induced spermatogenic damage in a microbiota dependent manner. We further found that the testicular tissue of both HS dairy goats and mice transplanted with HS dairy goat feces produced large amounts of arachidonic acid (AA)-related metabolites, which were closely associated with semen quality. Consistently, supplementation with AA has been shown to elevate the levels of inflammation and oxidative stress in the testicular tissue of mice, disrupting intercellular connections and ultimately leading to spermatogenic disorders.

Conclusion: This study has revealed that melatonin can effectively alleviate spermatogenic disorders in dairy goats caused by HS. This beneficial effect was primarily achieved through the modulation of gut microbiota, which subsequently inhibited the excessive synthesis of AA in testicular tissue. These discoveries are of great significance for preventing or improving the decline in male livestock reproductive performance caused by HS, enhancing the reproductive efficiency of elite male breeds, and ultimately improving the production efficiency of animal husbandry. Video Abstract.

背景:夏季常见的热应激(HS)已逐渐成为威胁雄性奶山羊繁殖性能的一个因素,会降低它们的受胎率。尽管褪黑素可用于缓解热应激,但褪黑素是否能保护奶山羊免受热应激引起的生殖损伤以及褪黑素是如何发挥作用的仍不清楚。本研究旨在评估褪黑素在缓解 HS 引起的雄性奶山羊精子发生功能障碍方面的作用,并进一步探讨其作用机制:结果:HS损害了奶山羊的精子发生、睾丸中精子的形成和附睾中精子的成熟,导致精子质量下降。褪黑素通过降低睾丸组织中的炎症和氧化应激水平以及增强睾丸内的细胞间屏障功能,缓解了HS导致的精子质量下降。基于扩增子的微生物群分析表明,尽管褪黑素处理过的奶山羊与NC奶山羊的肠道微生物群存在一定程度的差异,但褪黑素的施用可使HS作用下的雄性奶山羊的肠道微生物群恢复到自然对照奶山羊的水平。为了探索褪黑素对精子质量的保护作用是否是通过调节肠道微生物群介导的,我们将接受或未接受褪黑素治疗的 HS 奶山羊的粪便微生物群分别转移到 HS 小鼠体内。我们发现,接受了 HS 奶山羊粪便细菌的 HS 小鼠出现了严重的睾丸损伤和生精障碍,而接受了经褪黑素处理的 HS 奶山羊粪便细菌的 HS 小鼠的这一现象得到了改善,这表明褪黑素以微生物群依赖的方式减轻了 HS 诱导的生精损伤。我们进一步发现,HS奶山羊和移植了HS奶山羊粪便的小鼠的睾丸组织均产生大量花生四烯酸(AA)相关代谢物,这些代谢物与精液质量密切相关。同样,补充 AA 会提高小鼠睾丸组织的炎症和氧化应激水平,破坏细胞间的联系,最终导致生精障碍:本研究揭示了褪黑素可有效缓解 HS 引起的奶山羊生精障碍,这种有益作用主要是通过调节肠道微生物群来实现的,随后抑制了睾丸组织中 AA 的过度合成。这些发现对于预防或改善HS导致的雄性家畜繁殖性能下降,提高优良雄性品种的繁殖效率,最终提高畜牧业的生产效率具有重要意义。视频摘要
{"title":"Melatonin alleviates heat stress-induced spermatogenesis dysfunction in male dairy goats by regulating arachidonic acid metabolism mediated by remodeling the gut microbiota.","authors":"Xinrui Guo, Jing Xu, Yongkang Zhao, Jie Wang, Tingshu Fu, Mathias L Richard, Harry Sokol, Miao Wang, Yu Li, Yang Liu, Hui Wang, Chenlei Wang, Xueqin Wang, Haiyang He, Yazhou Wang, Baohua Ma, Sha Peng","doi":"10.1186/s40168-024-01942-6","DOIUrl":"10.1186/s40168-024-01942-6","url":null,"abstract":"<p><strong>Background: </strong>Heat stress (HS) commonly occurring in summer has gradually become a factor threatening the reproductive performance of male dairy goats by reducing their fecundity. Despite the melatonin is applied to relieve HS, it is still unclear whether melatonin protects against reproductive damage induced by HS in dairy goats and how it works. The purpose of the present study is to evaluate the role of melatonin in alleviating HS-induced spermatogenesis dysfunction in male dairy goats and further explore its mechanism.</p><p><strong>Results: </strong>HS impaired spermatogenesis, sperm formation in the testes, and sperm maturation in the epididymis of dairy goats, resulting in decreased sperm quality. Melatonin rescued the decrease of sperm quality induced by HS via decreasing inflammatory and oxidative stress levels in testicular tissue and enhancing intercellular barrier function within the testes. Amplicon-based microbiota analysis revealed that despite gut microbiota differences between melatonin-treated dairy goats and NC dairy goats to some extent, melatonin administration tends to return the gut microbiota of male dairy goats under HS to the levels of natural control dairy goats. To explore whether the protective role of melatonin in sperm quality is mediated by regulating gut microbiota, fecal microbiota of HS dairy goats with or without melatonin treatment were transferred to HS mice, respectively. We found HS mice that had received fecal bacteria of HS dairy goats experienced serious testicular injury and dyszoospermia, while this phenomenon was ameliorated in HS mice that had received fecal bacteria of dairy goats treated with melatonin, indicating melatonin alleviates HS-induced spermatogenic damage in a microbiota dependent manner. We further found that the testicular tissue of both HS dairy goats and mice transplanted with HS dairy goat feces produced large amounts of arachidonic acid (AA)-related metabolites, which were closely associated with semen quality. Consistently, supplementation with AA has been shown to elevate the levels of inflammation and oxidative stress in the testicular tissue of mice, disrupting intercellular connections and ultimately leading to spermatogenic disorders.</p><p><strong>Conclusion: </strong>This study has revealed that melatonin can effectively alleviate spermatogenic disorders in dairy goats caused by HS. This beneficial effect was primarily achieved through the modulation of gut microbiota, which subsequently inhibited the excessive synthesis of AA in testicular tissue. These discoveries are of great significance for preventing or improving the decline in male livestock reproductive performance caused by HS, enhancing the reproductive efficiency of elite male breeds, and ultimately improving the production efficiency of animal husbandry. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"12 1","pages":"233"},"PeriodicalIF":13.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Moving beyond species: fungal function in house dust provides novel targets for potential indicators of mold growth in homes. 超越物种:房屋灰尘中的真菌功能为家庭中霉菌生长的潜在指标提供了新的目标。
IF 13.8 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-09 DOI: 10.1186/s40168-024-01915-9
Neeraja Balasubrahmaniam, Jon C King, Bridget Hegarty, Karen C Dannemiller

Background: Increased risk of asthma and other respiratory diseases is associated with exposures to microbial communities growing in damp and moldy indoor environments. The exact causal mechanisms remain unknown, and occupant health effects have not been consistently associated with any species-based mold measurement methods. We need new quantitative methods to identify homes with potentially harmful fungal growth that are not dependent upon species. The goal of this study was to identify genes consistently associated with fungal growth and associated function under damp conditions for use as potential indicators of mold in homes regardless of fungal species present. A de novo metatranscriptomic analysis was performed using house dust from across the US, incubated at 50%, 85%, or 100% equilibrium relative humidity (ERH) for 1 week.

Results: Gene expression was a function of moisture (adonis2 p < 0.001), with fungal metabolic activity increasing with an increase in moisture condition (Kruskal-Wallis p = 0.003). Genes associated with fungal growth such as sporulation (n = 264), hyphal growth (n = 62), and secondary metabolism (n = 124) were significantly upregulated at elevated ERH conditions when compared to the low 50% ERH (FDR-adjusted p ≤ 0.001, log2FC ≥ 2), indicating that fungal function is influenced by damp conditions. A total of 67 genes were identified as consistently associated with the elevated 85% or 100% ERH conditions and included fungal developmental regulators and secondary metabolite genes such as brlA (log2FC = 7.39, upregulated at 100% compared to 85%) and stcC (log2FC = 8.78, upregulated at 85% compared to 50%).

Conclusions: Our results demonstrate that moisture conditions more strongly influence gene expression of indoor fungal communities compared to species presence. Identifying genes indicative of microbial growth under damp conditions will help develop robust monitoring techniques for indoor microbial exposures and improve understanding of how dampness and mold are linked to disease. Video Abstract.

背景:哮喘和其他呼吸道疾病风险的增加与暴露于潮湿和发霉的室内环境中生长的微生物群落有关。确切的成因机制仍不清楚,对居住者健康的影响也没有与任何基于物种的霉菌测量方法联系起来。我们需要新的定量方法来识别有潜在有害真菌生长的住宅,而不依赖于物种。本研究的目的是确定在潮湿条件下与真菌生长和相关功能一致的基因,以作为家庭霉菌的潜在指标,而不论存在的真菌种类。研究人员利用来自美国各地的房屋灰尘,在 50%、85% 或 100% 的平衡相对湿度(ERH)条件下培养 1 周,进行了全新的元转录组分析:基因表达是湿度的函数(adonis2 p 结论):我们的研究结果表明,与物种的存在相比,湿度条件对室内真菌群落基因表达的影响更大。确定潮湿条件下微生物生长的指示基因将有助于开发出可靠的室内微生物暴露监测技术,并加深人们对潮湿和霉菌如何与疾病相关的理解。视频摘要。
{"title":"Moving beyond species: fungal function in house dust provides novel targets for potential indicators of mold growth in homes.","authors":"Neeraja Balasubrahmaniam, Jon C King, Bridget Hegarty, Karen C Dannemiller","doi":"10.1186/s40168-024-01915-9","DOIUrl":"10.1186/s40168-024-01915-9","url":null,"abstract":"<p><strong>Background: </strong>Increased risk of asthma and other respiratory diseases is associated with exposures to microbial communities growing in damp and moldy indoor environments. The exact causal mechanisms remain unknown, and occupant health effects have not been consistently associated with any species-based mold measurement methods. We need new quantitative methods to identify homes with potentially harmful fungal growth that are not dependent upon species. The goal of this study was to identify genes consistently associated with fungal growth and associated function under damp conditions for use as potential indicators of mold in homes regardless of fungal species present. A de novo metatranscriptomic analysis was performed using house dust from across the US, incubated at 50%, 85%, or 100% equilibrium relative humidity (ERH) for 1 week.</p><p><strong>Results: </strong>Gene expression was a function of moisture (adonis2 p < 0.001), with fungal metabolic activity increasing with an increase in moisture condition (Kruskal-Wallis p = 0.003). Genes associated with fungal growth such as sporulation (n = 264), hyphal growth (n = 62), and secondary metabolism (n = 124) were significantly upregulated at elevated ERH conditions when compared to the low 50% ERH (FDR-adjusted p ≤ 0.001, log2FC ≥ 2), indicating that fungal function is influenced by damp conditions. A total of 67 genes were identified as consistently associated with the elevated 85% or 100% ERH conditions and included fungal developmental regulators and secondary metabolite genes such as brlA (log2FC = 7.39, upregulated at 100% compared to 85%) and stcC (log2FC = 8.78, upregulated at 85% compared to 50%).</p><p><strong>Conclusions: </strong>Our results demonstrate that moisture conditions more strongly influence gene expression of indoor fungal communities compared to species presence. Identifying genes indicative of microbial growth under damp conditions will help develop robust monitoring techniques for indoor microbial exposures and improve understanding of how dampness and mold are linked to disease. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"12 1","pages":"231"},"PeriodicalIF":13.8,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11549777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multimodal analysis identifies microbiome changes linked to stem cell transplantation-associated diseases. 多模式分析确定了与干细胞移植相关疾病有关的微生物组变化。
IF 4.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-07 DOI: 10.1186/s40168-024-01948-0
Alejandro Artacho, Cintya González-Torres, Nuria Gómez-Cebrián, Paula Moles-Poveda, Javier Pons, Nuria Jiménez, María Jinglei Casanova, Juan Montoro, Aitana Balaguer, Marta Villalba, Pedro Chorão, Leonor Puchades-Carrasco, Jaime Sanz, Carles Ubeda

Background: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is one of the most efficient therapeutic options available to cure many hematological malignancies. However, severe complications derived from this procedure, including graft-versus-host disease (GVHD) and infections, can limit its success and negatively impact survival. Previous studies have shown that alterations in the microbiome are associated with the development of allo-HSCT-derived complications. However, most studies relied on single techniques that can only analyze a unique aspect of the microbiome, which hinders our ability to understand how microbiome alterations drive allo-HSCT-associated diseases.

Results: Here, we have applied multiple "omic" techniques (16S rRNA and shotgun sequencing, targeted and un-targeted metabolomics) in combination with machine learning approaches to define the most significant microbiome changes following allo-HSCT at multiple modalities (bacterial taxa, encoded functions, and derived metabolites). In addition, multivariate approaches were applied to study interactions among the various microbiome modalities (the interactome). Our results show that the microbiome of transplanted patients exhibits substantial changes in all studied modalities. These include depletion of beneficial microbes, mainly from the Clostridiales order, loss of their bacterial encoded functions required for the synthesis of key metabolites, and a reduction in metabolic end products such as short chain fatty acids (SCFAs). These changes were followed by an expansion of bacteria that frequently cause infections after allo-HSCT, including several Staphylococcus species, which benefit from the reduction of bacteriostatic SCFAs. Additionally, we found specific alterations in all microbiome modalities that distinguished those patients who subsequently developed GVHD, including depletion of anti-inflammatory commensals, protective reactive oxygen detoxifying enzymes, and immunoregulatory metabolites such as acetate or malonate. Moreover, extensive shifts in the homeostatic relationship between bacteria and their metabolic products (e.g., Faecalibacterium and butyrate) were detected mainly in patients who later developed GVHD.

Conclusions: We have identified specific microbiome changes at different modalities (microbial taxa, their encoded genes, and synthetized metabolites) and at the interface between them (the interactome) that precede the development of complications associated with allo-HSCT. These identified microbial features provide novel targets for the design of microbiome-based strategies to prevent diseases associated with stem cell transplantation. Video Abstract.

背景:异基因造血干细胞移植(allo-HSCT异基因造血干细胞移植(allo-HSCT)是治疗多种血液恶性肿瘤最有效的方法之一。然而,移植抗宿主疾病(GVHD)和感染等严重并发症会限制移植的成功率,并对存活率产生负面影响。以往的研究表明,微生物组的改变与allo-HSCT衍生并发症的发生有关。然而,大多数研究依赖于只能分析微生物组某一独特方面的单一技术,这阻碍了我们了解微生物组的改变如何驱动allo-HSCT相关疾病的能力:在这里,我们应用了多种 "omic "技术(16S rRNA和霰弹枪测序、靶向和非靶向代谢组学),结合机器学习方法,通过多种模式(细菌类群、编码功能和衍生代谢物)确定了allo-HSCT后微生物组最显著的变化。此外,我们还采用多元方法研究了各种微生物组模式之间的相互作用(交互组)。我们的研究结果表明,移植患者的微生物组在所有研究模式中都发生了重大变化。这些变化包括有益微生物(主要是梭状芽孢杆菌)的减少、合成关键代谢物所需的细菌编码功能的丧失以及代谢终产物(如短链脂肪酸)的减少。在发生这些变化后,异体 HSCT 后经常引起感染的细菌也随之增多,其中包括几种葡萄球菌,它们从抑菌性 SCFAs 的减少中获益。此外,我们还发现所有微生物组模式都发生了特异性改变,这些改变将随后发生 GVHD 的患者区分开来,包括抗炎共生菌、保护性活性氧解毒酶和免疫调节代谢物(如醋酸盐或丙二酸盐)的耗竭。此外,细菌及其代谢产物(如粪杆菌和丁酸盐)之间的平衡关系也发生了广泛变化,这种变化主要出现在后来发生 GVHD 的患者身上:我们发现了不同模式(微生物类群、其编码基因和合成代谢产物)和它们之间的界面(相互作用组)上的特定微生物组变化,这些变化发生在发生与异体造血干细胞移植相关的并发症之前。这些已确定的微生物特征为设计基于微生物的策略预防干细胞移植相关疾病提供了新的目标。视频摘要。
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引用次数: 0
Medium-chain fatty acid triglycerides improve feed intake and oxidative stress of finishing bulls by regulating ghrelin concentration and gastrointestinal tract microorganisms and rumen metabolites. 中链脂肪酸甘油三酯通过调节胃泌素浓度、胃肠道微生物和瘤胃代谢物,提高育成公牛的采食量和氧化应激。
IF 4.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-07 DOI: 10.1186/s40168-024-01946-2
Jiaming Luan, Xin Feng, Yunlong Du, Dongxu Yang, Chunyin Geng
<p><strong>Background: </strong>As a feed additive, medium-chain fatty acids (MCFAs)/medium-chain fatty acid triglycerides (MCTs) have been used in ruminant production, but mostly added in the form of mixed esters. Studies have shown that MCTs may have a positive effect on feed intake or oxidative stress in animals, but it is unclear which MCT could play a role, and the mechanism has not been elucidated. In this study, the effects of individual MCT on growth performance, serum intake-related hormones, and oxidative stress indices in finishing bulls were investigated and further studied the effects of MCT supplementation on gastrointestinal tract bacteria and rumen fluid metabolomics.</p><p><strong>Results: </strong>Four ruminally fistulated Yanbian cattle (bulls) were selected in 4 × 4 Latin square designs and allocated to four treatment groups: a control group (CON) fed a basal diet (total mixed ration, TMR), three groups fed a basal diet supplemented with 60 g/bull/day glycerol monocaprylin (GMC, C8), glycerol monodecanoate (GMD, C10), and glycerol monolaurate (GML, C12), respectively. Compared with the CON group, GMD tended to increase the dry matter intake (DMI) of finishing bulls (P = 0.069). Compared with the CON group, GMD significantly increased the concentration of ghrelin O-acyl transferase (GOAT), total ghrelin (TG), acylated ghrelin (AG), and orexins (P < 0.05) and significantly decreased the concentrations of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde, reactive oxygen species (ROS), and lipopolysaccharides (LPS) in the serum of finishing bulls (P < 0.05). Compared with the CON group, GMD and GML significantly increased the concentrations of total antioxidant capacity (T-AOC), catalase, glutathione peroxidase (GSH-PX), glutathione reductase (GR), and nitric oxide (NO) in the serum of finishing bulls (P < 0.05). Compared with the CON group, there were 5, 14, and 6 significantly different bacteria in the rumen digesta in the C8, C10, and C12 groups, respectively; there were 3, 10, and 5 significantly different bacteria in the rumen fluid in the C8, C10, and C12 groups, respectively; and only one differential bacteria (genus level) in the feces among the four treatment groups. Compared with the CON group, there were 3, 14, and 15 significantly differential metabolites identified under positive ionization mode in the C8, C10, and C12 groups, respectively, while under negative ionization mode were 3, 11 and 14, respectively. Correlation analysis showed that there was a significant correlation between DMI, GOAT, AG, GSH-PX, LPS, gastrointestinal tract bacteria, and rumen fluid metabolites.</p><p><strong>Conclusions: </strong>Our findings revealed that different types of MCTs have different application effects in ruminants. Among them, GMD may improve the feed intake of finishing bulls by stimulating the secretion of AG. GMD and GML may change gastrointestinal tract microorganisms and produce specific rumen metabolites t
背景:作为一种饲料添加剂,中链脂肪酸(MCFAs)/中链脂肪酸甘油三酯(MCTs)已被用于反刍动物生产,但大多以混合酯的形式添加。研究表明,MCTs 可能对动物的饲料摄入量或氧化应激有积极影响,但目前尚不清楚哪种 MCT 可能起作用,其机制也未阐明。本研究调查了单个 MCT 对育成公牛生长性能、血清采食量相关激素和氧化应激指标的影响,并进一步研究了补充 MCT 对胃肠道细菌和瘤胃液代谢组学的影响:按4×4拉丁方阵设计选取4头反刍的延边牛(公牛),将其分配到4个处理组:1个对照组(CON)饲喂基础日粮(全混合日粮,TMR),3个组分别饲喂添加60克/头/天甘油单癸酸酯(GMC,C8)、甘油单癸酸酯(GMD,C10)和甘油单月桂酸酯(GML,C12)的基础日粮。与 CON 组相比,GMD 有增加育成公牛干物质摄入量(DMI)的趋势(P = 0.069)。与 CON 组相比,GMD 能显著增加育成公牛血清中胃泌素 O-酰基转移酶(GOAT)、总胃泌素(TG)、酰化胃泌素(AG)、奥曲肽(P 2O2)、丙二醛、活性氧(ROS)和脂多糖(LPS)的浓度(P 结论):我们的研究结果表明,不同类型的 MCT 对反刍动物有不同的应用效果。其中,GMD 可通过刺激 AG 的分泌来提高育成公牛的采食量。GMD 和 GML 可改变胃肠道微生物并产生特定的瘤胃代谢物,从而改善育成公牛的氧化应激,胃泌素也可能参与其中。本研究揭示了 MCT 改善育成公牛采食量和氧化应激的潜在机制。视频摘要。
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引用次数: 0
MAGqual: a stand-alone pipeline to assess the quality of metagenome-assembled genomes. MAGqual:评估元基因组组装基因组质量的独立管道。
IF 13.8 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-04 DOI: 10.1186/s40168-024-01949-z
Annabel Cansdale, James P J Chong

Background: Metagenomics, the whole genome sequencing of microbial communities, has provided insight into complex ecosystems. It has facilitated the discovery of novel microorganisms, explained community interactions and found applications in various fields. Advances in high-throughput and third-generation sequencing technologies have further fuelled its popularity. Nevertheless, managing the vast data produced and addressing variable dataset quality remain ongoing challenges. Another challenge arises from the number of assembly and binning strategies used across studies. Comparing datasets and analysis tools is complex as it requires the quantitative assessment of metagenome quality. The inherent limitations of metagenomic sequencing, which often involves sequencing complex communities, mean community members are challenging to interrogate with traditional culturing methods leading to many lacking reference sequences. MIMAG standards aim to provide a method to assess metagenome quality for comparison but have not been widely adopted.

Results: To address the need for simple and quick metagenome quality assignation, here we introduce the pipeline MAGqual (Metagenome-Assembled Genome qualifier) and demonstrate its effectiveness at determining metagenomic dataset quality in the context of the MIMAG standards.

Conclusions: The MAGqual pipeline offers an accessible way to evaluate metagenome quality and generate metadata on a large scale. MAGqual is built in Snakemake to ensure readability and scalability, and its open-source nature promotes accessibility, community development, and ease of updates. MAGqual is built in Snakemake, R, and Python and is available under the MIT license on GitHub at https://github.com/ac1513/MAGqual . Video Abstract.

背景:元基因组学是对微生物群落进行全基因组测序的方法,它有助于人们深入了解复杂的生态系统。它促进了新型微生物的发现,解释了群落间的相互作用,并在各个领域得到了应用。高通量和第三代测序技术的进步进一步推动了它的普及。然而,管理所产生的大量数据和解决数据集质量参差不齐的问题仍然是持续存在的挑战。另一个挑战来自于不同研究中使用的组装和分选策略的数量。比较数据集和分析工具非常复杂,因为这需要对元基因组质量进行定量评估。元基因组测序通常涉及复杂群落的测序,其固有的局限性意味着用传统的培养方法询问群落成员具有挑战性,导致许多群落缺乏参考序列。MIMAG 标准旨在提供一种评估元基因组质量的方法,以进行比较,但尚未被广泛采用:为了满足对简单、快速的元基因组质量评估的需求,我们在此介绍了MAGqual(元基因组组装基因组限定器)管道,并展示了它在MIMAG标准背景下确定元基因组数据集质量的有效性:MAGqual 管道为大规模评估元基因组质量和生成元数据提供了一种简便易行的方法。MAGqual 是在 Snakemake 中构建的,以确保可读性和可扩展性,其开源性质促进了可访问性、社区开发和易于更新。MAGqual 使用 Snakemake、R 和 Python 构建,在 MIT 许可下可在 GitHub 上获取:https://github.com/ac1513/MAGqual 。视频摘要。
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引用次数: 0
Intestinal commensal bacteria promote Bactrocera dorsalis larval development through the vitamin B6 synthesis pathway. 肠道共生细菌通过维生素 B6 合成途径促进背鳍乳杆菌幼虫的发育。
IF 13.8 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-04 DOI: 10.1186/s40168-024-01931-9
Jian Gu, Zhichao Yao, Bruno Lemaitre, Zhaohui Cai, Hongyu Zhang, Xiaoxue Li

Background: The gut microbiota can facilitate host growth under nutrient-constrained conditions. However, whether this effect is limited to certain bacterial species remains largely unclear, and the relevant underlying mechanisms remain to be thoroughly investigated.

Results: We found that the microbiota was required for Bactrocera dorsalis larval growth under poor dietary conditions. Monoassociation experiments revealed that Enterobacteriaceae and some Lactobacilli promoted larval growth. Among the 27 bacterial strains tested, 14 significantly promoted larval development, and the Enterobacteriaceae cloacae isolate exhibited the most obvious promoting effect. A bacterial genome-wide association study (GWAS) revealed that the vitamin B6 synthesis pathway was critical for the promotion of E. cloacae growth. Deletion of pdxA, which is responsible for vitamin B6 biosynthesis, deprived the mutant strains of larval growth-promoting function, indicating that the 4-hydroxythreonine-4-phosphate dehydrogenase(pdxA) gene was crucial for promoting larval growth in E. cloacae. Importantly, supplementation of a poor diet with vitamin B6 successfully rescued the axenic larval growth phenotype of B. dorsalis.

Conclusion: Our results suggest that gut microbes promote insect larval growth by providing vitamin B6 under nutrient scarcity conditions in B. dorsalis. Video Abstract.

背景:肠道微生物群可在营养受限的条件下促进宿主的生长。然而,这种作用是否仅限于某些细菌种类在很大程度上仍不清楚,相关的内在机制仍有待深入研究:结果:我们发现,在恶劣的饮食条件下,背鳍乳杆菌幼虫的生长需要微生物群。单菌落实验表明,肠杆菌科细菌和一些乳酸杆菌能促进幼虫的生长。在测试的 27 株细菌中,有 14 株能显著促进幼虫发育,其中泄殖腔肠杆菌属分离株的促进作用最为明显。细菌全基因组关联研究(GWAS)发现,维生素 B6 合成途径对促进泄殖腔肠杆菌的生长至关重要。缺失了负责维生素 B6 生物合成的 pdxA 基因,突变株就失去了促进幼虫生长的功能,这表明 4-羟基苏氨酸-4-磷酸脱氢酶(pdxA)基因对促进泄殖酵母幼虫的生长至关重要。重要的是,在贫乏的饮食中补充维生素 B6 成功地挽救了 B. dorsalis 的轴突幼虫生长表型:我们的研究结果表明,在营养匮乏的条件下,肠道微生物通过提供维生素 B6 促进了背甲线虫幼虫的生长。视频摘要
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引用次数: 0
Nepali oral microbiomes reflect a gradient of lifestyles from traditional to industrialized. 尼泊尔人的口腔微生物组反映了从传统到工业化的梯度生活方式。
IF 13.8 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-04 DOI: 10.1186/s40168-024-01941-7
Erica P Ryu, Yoshina Gautam, Diana M Proctor, Dinesh Bhandari, Sarmila Tandukar, Meera Gupta, Guru Prasad Gautam, David A Relman, Ahmed A Shibl, Jeevan Bahadur Sherchand, Aashish R Jha, Emily R Davenport

Background: Lifestyle plays an important role in shaping the gut microbiome. However, its contributions to the oral microbiome remain less clear, due to the confounding effects of geography and methodology in investigations of populations studied to date. Furthermore, while the oral microbiome seems to differ between foraging and industrialized populations, we lack insight into whether transitions to and away from agrarian lifestyles shape the oral microbiota. Given the growing interest in so-called "vanishing microbiomes" potentially being a risk factor for increased disease prevalence in industrialized populations, it is important that we distinguish lifestyle from geography in the study of microbiomes across populations.

Results: Here, we investigate salivary microbiomes of 63 Nepali individuals representing a spectrum of lifestyles: foraging, subsistence farming (individuals that transitioned from foraging to farming within the last 50 years), agriculturalists (individuals that have transitioned to farming for at least 300 years), and industrialists (expatriates that immigrated to the USA within the last 20 years). We characterize the role of lifestyle in microbial diversity, identify microbes that differ between lifestyles, and pinpoint specific lifestyle factors that may be contributing to differences in the microbiomes across populations. Contrary to prevailing views, when geography is controlled for, oral microbiome alpha diversity does not differ significantly across lifestyles. Microbiome composition, however, follows the gradient of lifestyles from foraging through agrarianism to industrialism, supporting the notion that lifestyle indeed plays a role in the oral microbiome. Relative abundances of several individual taxa, including Streptobacillus and an unclassified Porphyromonadaceae genus, also mirror lifestyle. Finally, we identify specific lifestyle factors associated with microbiome composition across the gradient of lifestyles, including smoking and grain sources.

Conclusion: Our findings demonstrate that by studying populations within Nepal, we can isolate an important role of lifestyle in determining oral microbiome composition. In doing so, we highlight the potential contributions of several lifestyle factors, underlining the importance of carefully examining the oral microbiome across lifestyles to improve our understanding of global microbiomes. Video Abstract.

背景:生活方式在塑造肠道微生物组方面发挥着重要作用。然而,由于迄今为止对人群的调查中存在地理和方法的混淆效应,其对口腔微生物组的贡献仍然不太清楚。此外,虽然觅食人群和工业化人群的口腔微生物组似乎有所不同,但我们对农业生活方式的转变是否会影响口腔微生物组还缺乏深入了解。鉴于所谓的 "消失的微生物群 "可能是工业化人群疾病发病率增加的一个风险因素这一观点越来越受到关注,我们在研究不同人群的微生物群时有必要将生活方式与地理环境区分开来:在这里,我们研究了 63 名尼泊尔人的唾液微生物组,他们代表了不同的生活方式:觅食者、自给农耕者(在过去 50 年内从觅食过渡到农耕的个体)、农耕者(过渡到农耕至少 300 年的个体)和工业者(在过去 20 年内移民到美国的外籍人士)。我们描述了生活方式在微生物多样性中的作用,确定了不同生活方式中的微生物差异,并指出了可能导致不同人群微生物组差异的特定生活方式因素。与普遍观点相反,当地理因素受到控制时,口腔微生物组阿尔法多样性在不同生活方式之间并无显著差异。然而,微生物组的组成遵循了从觅食到农耕再到工业化的生活方式梯度,支持了生活方式确实在口腔微生物组中发挥作用的观点。包括链球菌和一个未分类的卟啉单胞菌属在内的几个类群的相对丰度也反映了生活方式。最后,我们确定了与整个生活方式梯度中微生物组组成相关的特定生活方式因素,包括吸烟和谷物来源:我们的研究结果表明,通过研究尼泊尔国内的人群,我们可以分离出生活方式在决定口腔微生物组组成中的重要作用。在此过程中,我们强调了几种生活方式因素的潜在作用,强调了仔细研究不同生活方式下的口腔微生物组以提高我们对全球微生物组的了解的重要性。视频摘要。
{"title":"Nepali oral microbiomes reflect a gradient of lifestyles from traditional to industrialized.","authors":"Erica P Ryu, Yoshina Gautam, Diana M Proctor, Dinesh Bhandari, Sarmila Tandukar, Meera Gupta, Guru Prasad Gautam, David A Relman, Ahmed A Shibl, Jeevan Bahadur Sherchand, Aashish R Jha, Emily R Davenport","doi":"10.1186/s40168-024-01941-7","DOIUrl":"10.1186/s40168-024-01941-7","url":null,"abstract":"<p><strong>Background: </strong>Lifestyle plays an important role in shaping the gut microbiome. However, its contributions to the oral microbiome remain less clear, due to the confounding effects of geography and methodology in investigations of populations studied to date. Furthermore, while the oral microbiome seems to differ between foraging and industrialized populations, we lack insight into whether transitions to and away from agrarian lifestyles shape the oral microbiota. Given the growing interest in so-called \"vanishing microbiomes\" potentially being a risk factor for increased disease prevalence in industrialized populations, it is important that we distinguish lifestyle from geography in the study of microbiomes across populations.</p><p><strong>Results: </strong>Here, we investigate salivary microbiomes of 63 Nepali individuals representing a spectrum of lifestyles: foraging, subsistence farming (individuals that transitioned from foraging to farming within the last 50 years), agriculturalists (individuals that have transitioned to farming for at least 300 years), and industrialists (expatriates that immigrated to the USA within the last 20 years). We characterize the role of lifestyle in microbial diversity, identify microbes that differ between lifestyles, and pinpoint specific lifestyle factors that may be contributing to differences in the microbiomes across populations. Contrary to prevailing views, when geography is controlled for, oral microbiome alpha diversity does not differ significantly across lifestyles. Microbiome composition, however, follows the gradient of lifestyles from foraging through agrarianism to industrialism, supporting the notion that lifestyle indeed plays a role in the oral microbiome. Relative abundances of several individual taxa, including Streptobacillus and an unclassified Porphyromonadaceae genus, also mirror lifestyle. Finally, we identify specific lifestyle factors associated with microbiome composition across the gradient of lifestyles, including smoking and grain sources.</p><p><strong>Conclusion: </strong>Our findings demonstrate that by studying populations within Nepal, we can isolate an important role of lifestyle in determining oral microbiome composition. In doing so, we highlight the potential contributions of several lifestyle factors, underlining the importance of carefully examining the oral microbiome across lifestyles to improve our understanding of global microbiomes. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"12 1","pages":"228"},"PeriodicalIF":13.8,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The gut microbiome in patients with Cushing's disease affects depression- and anxiety-like behavior in mice. 库欣病患者的肠道微生物群会影响小鼠的抑郁和焦虑行为。
IF 13.8 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-11-01 DOI: 10.1186/s40168-024-01939-1
Ding Nie, Dawei Wang, Zhenhua Wang, Qiuyue Fang, Hongyun Wang, Weiyan Xie, Chuzhong Li, Yazhuo Zhang

Background: Depression and anxiety significantly impact the quality of life in individuals with Cushing's disease (CD), which originates from pituitary neuroendocrine tumors (PitNETs), yet our understanding of the underlying mechanisms is limited. There is substantial evidence linking gut microbes to depression, anxiety, and endocrinology.

Results: The gut bacterial phenotype of patients with Cushing's disease was significantly different from that of the control group, and when the mice were treated with fecal bacteria from these patients, both anxiety- and depression-like behavior were significantly increased. However, this effect can be alleviated by supplementing with 2-(14, 15-epoxyeicosatrienoyl) glycerol (2-14,15-EG) which was found at reduced levels in the peripheral blood of mice treated with coprofecal bacteria from Cushing's disease. In this process, the effects of hormone levels and immune factors were not significant. In addition, in an animal model, corticosterone has been observed to affect behavioral changes in mice through gut microbiota composition, clarifying the cause-and-effect relationship between hormones, microbiota, and behavior. Finally, there was no significant difference in gut microbiome composition and its effects on mouse behavior in patients with Cushing's disease with different levels of depression and anxiety.

Conclusions: In summary, this research enhances our current understanding of how gut microbes in patients with Cushing's disease contribute to depression and anxiety, offering novel insights for clinical treatment approaches. Video Abstract.

背景:抑郁和焦虑严重影响着库欣病(CD)患者的生活质量,而库欣病是由垂体神经内分泌肿瘤(PitNETs)引起的,但我们对其潜在机制的了解却很有限。有大量证据表明肠道微生物与抑郁、焦虑和内分泌有关:库欣病患者的肠道细菌表型与对照组有显著差异,用这些患者的粪便细菌处理小鼠时,焦虑和抑郁样行为都会显著增加。然而,通过补充 2-(14,15-环氧洋三烯酰基)甘油(2-14,15-EG)可减轻这种影响,在使用库欣病共排泄物细菌治疗的小鼠外周血中,2-(14,15-环氧洋三烯酰基)甘油的含量有所降低。在这一过程中,激素水平和免疫因素的影响并不显著。此外,在一个动物模型中,观察到皮质酮通过肠道微生物群的组成影响小鼠的行为变化,明确了激素、微生物群和行为之间的因果关系。最后,不同抑郁和焦虑程度的库欣病患者的肠道微生物群组成及其对小鼠行为的影响没有明显差异:总之,这项研究加深了我们目前对库欣病患者肠道微生物如何导致抑郁和焦虑的理解,为临床治疗方法提供了新的见解。视频摘要
{"title":"The gut microbiome in patients with Cushing's disease affects depression- and anxiety-like behavior in mice.","authors":"Ding Nie, Dawei Wang, Zhenhua Wang, Qiuyue Fang, Hongyun Wang, Weiyan Xie, Chuzhong Li, Yazhuo Zhang","doi":"10.1186/s40168-024-01939-1","DOIUrl":"10.1186/s40168-024-01939-1","url":null,"abstract":"<p><strong>Background: </strong>Depression and anxiety significantly impact the quality of life in individuals with Cushing's disease (CD), which originates from pituitary neuroendocrine tumors (PitNETs), yet our understanding of the underlying mechanisms is limited. There is substantial evidence linking gut microbes to depression, anxiety, and endocrinology.</p><p><strong>Results: </strong>The gut bacterial phenotype of patients with Cushing's disease was significantly different from that of the control group, and when the mice were treated with fecal bacteria from these patients, both anxiety- and depression-like behavior were significantly increased. However, this effect can be alleviated by supplementing with 2-(14, 15-epoxyeicosatrienoyl) glycerol (2-14,15-EG) which was found at reduced levels in the peripheral blood of mice treated with coprofecal bacteria from Cushing's disease. In this process, the effects of hormone levels and immune factors were not significant. In addition, in an animal model, corticosterone has been observed to affect behavioral changes in mice through gut microbiota composition, clarifying the cause-and-effect relationship between hormones, microbiota, and behavior. Finally, there was no significant difference in gut microbiome composition and its effects on mouse behavior in patients with Cushing's disease with different levels of depression and anxiety.</p><p><strong>Conclusions: </strong>In summary, this research enhances our current understanding of how gut microbes in patients with Cushing's disease contribute to depression and anxiety, offering novel insights for clinical treatment approaches. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"12 1","pages":"225"},"PeriodicalIF":13.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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