Pub Date : 2024-04-02DOI: 10.1134/s0026261723604323
S. Yu. Shchyogolev, G. L. Burygin, Yu. V. Krasova, L. Yu. Matora
Abstract
Conserved motifs (peptide patterns) determining the elicitor properties of plant-pathogenic bacteria were identified in the amino acid sequences of the flagellins of phytopathogenic, associative, and root nodule microflora. In plant-growth-promoting rhizobacteria (PGPR), analogs of one (flg22) out of two (flg22 and flgII-28) specific peptides characteristic of pathogens were found. Instead of glycine G18, characteristic of an elicitor, tyrosine Y18 was identified in flg22 analogs of most PGPR, which prevents actuation of the phytoimmunity mechanism against PGPR. Molecular docking with the AlphaFold software package demonstrated significance of the interaction between the plant receptor FLS2 and the canonical peptide flg22 and its analogs from a plant pathogen and an Azospirillum bacterium. However, in the case of the FLS3 plant receptor, only its interactions with the canonical peptide flgII-28 and its analog from the plant pathogen were statistically significant.
{"title":"The MAMP Peptide Patterns of Bacterial Flagellins and Their Interaction with Plant Receptors: Bioinformatic and Coevolutionary Aspects","authors":"S. Yu. Shchyogolev, G. L. Burygin, Yu. V. Krasova, L. Yu. Matora","doi":"10.1134/s0026261723604323","DOIUrl":"https://doi.org/10.1134/s0026261723604323","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>\u0000</h3><p>Conserved motifs (peptide patterns) determining the elicitor properties of plant-pathogenic bacteria were identified in the amino acid sequences of the flagellins of phytopathogenic, associative, and root nodule microflora. In plant-growth-promoting rhizobacteria (PGPR), analogs of one (flg22) out of two (flg22 and flgII-28) specific peptides characteristic of pathogens were found. Instead of glycine G18, characteristic of an elicitor, tyrosine Y18 was identified in flg22 analogs of most PGPR, which prevents actuation of the phytoimmunity mechanism against PGPR. Molecular docking with the AlphaFold software package demonstrated significance of the interaction between the plant receptor FLS2 and the canonical peptide flg22 and its analogs from a plant pathogen and an <i>Azospirillum</i> bacterium. However, in the case of the FLS3 plant receptor, only its interactions with the canonical peptide flgII-28 and its analog from the plant pathogen were statistically significant.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1134/s0026261723603561
O. S. Samylina, O. I. Gabysheva, V. A. Gabyshev, V. V. Kadnikov, A. V. Beletsky, A. I. Kosyakova, A. Yu. Kallistova, N. V. Pimenov
Abstract
Although thermokarst alas lakes of Central Yakutia are of great climatic and economic importance, there is currently virtually no information on microbial communities and microbial processes in these lakes. This paper characterizes the hydrochemical features and presents a primary analysis of the diversity of planktonic microbial communities in three alas lakes of Central Yakutia—Tyungulyu, Taby, and Kharyyalakh. It was shown that in terms of the water physicochemical composition, the studied lakes were quite typical for this region; they had increased alkalinity and trophicity, but differed from each other in microbiological indicators. Chemoheterotrophic prokaryotes predominated in the studied planktonic communities, but a significant proportion of the 16S rRNA gene sequences were most similar to uncultured microorganisms whose functional potential is still unknown.
{"title":"Planktonic Microbial Communities of Thermokarst Lakes of Central Yakutia Demonstrate a High Diversity of Uncultivated Prokaryotes with Uncharacterized Functions","authors":"O. S. Samylina, O. I. Gabysheva, V. A. Gabyshev, V. V. Kadnikov, A. V. Beletsky, A. I. Kosyakova, A. Yu. Kallistova, N. V. Pimenov","doi":"10.1134/s0026261723603561","DOIUrl":"https://doi.org/10.1134/s0026261723603561","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>\u0000</h3><p>Although thermokarst alas lakes of Central Yakutia are of great climatic and economic importance, there is currently virtually no information on microbial communities and microbial processes in these lakes. This paper characterizes the hydrochemical features and presents a primary analysis of the diversity of planktonic microbial communities in three alas lakes of Central Yakutia—Tyungulyu, Taby, and Kharyyalakh. It was shown that in terms of the water physicochemical composition, the studied lakes were quite typical for this region; they had increased alkalinity and trophicity, but differed from each other in microbiological indicators. Chemoheterotrophic prokaryotes predominated in the studied planktonic communities, but a significant proportion of the 16S rRNA gene sequences were most similar to uncultured microorganisms whose functional potential is still unknown.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140884150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1134/s0026261723604062
T. N. Kuvichkina, E. N. Kaparullina, N. V. Doronina, A. N. Reshetilov
Abstract
The possible application of Rhоdococcus qingshengii strain GlMm1, isolated from a Dead Sea clay sample, as the basis of a biosensor for determining the benzimidazole fungicide carbendazim was investigated. High sensitivity of the biosensor under neutral pH and up to 500 mM NaCl at 2 to 160 μM carbendazim was maintained for up to 30 days.
{"title":"Rhodococcus qingshengii GlMm1 as the Basis for a Biosensor for Determination of the Fungicide Carbendazim","authors":"T. N. Kuvichkina, E. N. Kaparullina, N. V. Doronina, A. N. Reshetilov","doi":"10.1134/s0026261723604062","DOIUrl":"https://doi.org/10.1134/s0026261723604062","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>\u0000</h3><p>The possible application of <i>Rhоdococcus qingshengii</i> strain GlMm1, isolated from a Dead Sea clay sample, as the basis of a biosensor for determining the benzimidazole fungicide carbendazim was investigated. High sensitivity of the biosensor under neutral pH and up to 500 mM NaCl at 2 to 160 μM carbendazim was maintained for up to 30 days.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1134/s0026261723603913
D. N. Tekucheva, M. V. Karpov, V. V. Fokina, T. A. Timakova, A. A. Shutov, M. V. Donova
Abstract
A plasmid containing the genes encoding a fungal 17β-hydroxysteroid dehydrogenase, which ca-talyzes the reduction of 17-oxo group, and a mycobacterial glucose-6-phosphate dehydrogenase, which promotes the recycling of the essential coenzyme NAD(P)H, was constructed. Its constitutive expression in well-studied Mycolicibacterium neoaurum strains made it possible to increase the yield of C-17 hydroxysteroids significantly. In particular, recombinant strains created on the basis of M. neoaurum VKM Ac-1815D and M. neoaurum NRRL B-3805 ΔkstD exhibited predominant accumulation of testosterone, while the strain based on M. neoaurum VKM Ac-1816D accumulated 1(2)-dehydrotestosterone and testosterone simultaneously.
{"title":"Single-Stage Bioconversion of Phytosterol into Testosterone by Recombinant Strains of Mycolicibacterium neoaurum","authors":"D. N. Tekucheva, M. V. Karpov, V. V. Fokina, T. A. Timakova, A. A. Shutov, M. V. Donova","doi":"10.1134/s0026261723603913","DOIUrl":"https://doi.org/10.1134/s0026261723603913","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>\u0000</h3><p>A plasmid containing the genes encoding a fungal 17β-hydroxysteroid dehydrogenase, which ca-talyzes the reduction of 17-oxo group, and a mycobacterial glucose-6-phosphate dehydrogenase, which promotes the recycling of the essential coenzyme NAD(P)H, was constructed. Its constitutive expression in well-studied <i>Mycolicibacterium neoaurum</i> strains made it possible to increase the yield of C-17 hydroxysteroids significantly. In particular, recombinant strains created on the basis of <i>M. neoaurum</i> VKM Ac-1815D and <i>M. neoaurum</i> NRRL B-3805 Δ<i>kstD</i> exhibited predominant accumulation of testosterone, while the strain based on <i>M. neoaurum</i> VKM Ac-1816D accumulated 1(2)-dehydrotestosterone and testosterone simultaneously.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1134/s0026261723604335
N. A. Manucharova, A. P. Vlasova, M. A. Kovalenko, E. A. Ovchinnikova, A. D. Babenko, G. A. Teregulova, G. V. Uvarov, A. L. Stepanov
Abstract
Molecular biological techniques and bioinformatic analysis were used to investigate the phylogenetic and functional diversity of the prokaryotic complex of soil microcosms. The dominant organisms of the hydrolytic community were different in the samples from different climatic zones. In the soils subject to anthropogenic or abiogenic load, apart from decreased diversity and abundance of prokaryotes, the number of the genes marking the ability to degrade xenobiotics, as well as those encoding nitrogen conversion and metabolism of vitamins and cofactors, was found to increase. Under heavy oil contamination, the bacterial community was capable of nitrification; its role increased in the lower horizons of the soil profile. The patterns revealed in the work indicate high metabolic potential of the prokaryotic component of the studied soils.
{"title":"Biotechnological Potential of the Soil Microbiome","authors":"N. A. Manucharova, A. P. Vlasova, M. A. Kovalenko, E. A. Ovchinnikova, A. D. Babenko, G. A. Teregulova, G. V. Uvarov, A. L. Stepanov","doi":"10.1134/s0026261723604335","DOIUrl":"https://doi.org/10.1134/s0026261723604335","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>\u0000</h3><p>Molecular biological techniques and bioinformatic analysis were used to investigate the phylogenetic and functional diversity of the prokaryotic complex of soil microcosms. The dominant organisms of the hydrolytic community were different in the samples from different climatic zones. In the soils subject to anthropogenic or abiogenic load, apart from decreased diversity and abundance of prokaryotes, the number of the genes marking the ability to degrade xenobiotics, as well as those encoding nitrogen conversion and metabolism of vitamins and cofactors, was found to increase. Under heavy oil contamination, the bacterial community was capable of nitrification; its role increased in the lower horizons of the soil profile. The patterns revealed in the work indicate high metabolic potential of the prokaryotic component of the studied soils.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1134/s0026261723603974
M. Yu. Suslova, G. V. Podlesnaya, I. V. Tomberg, M. V. Sakirko, O. I. Belykh
Abstract
Phosphorus is one of the major biogenic elements. Its inflow facilitates eutrophication of lake water. In aquatic ecosystems, phosphorus is present mostly in organic compounds. Ability of aquatic microorganisms to assimilate phosphorus from organophosphorous compounds results from activity of alkaline phosphatases; activity of these enzymes may be an indicator of the state of the ecosystem, phosphate load, and water quality. In the present work, alkaline phosphatase activity (APA) and abundance of phosphatase-active bacteria (PAB) in Lake Baikal pelagic zone and in the mouths of its major tributaries was studied. In the pelagic zone, APA and PAB abundance decreased with depth, indicating that the main processes of phosphate generation occurred in the trophic layer of the lake. In the main tributaries, both APA and PAB abundance were considerably higher than in the pelagic zone. These results indicate active biochemical processes of transformation of organophosphorous compounds occur in the estuarine zones of the rivers. The degradation processes result in regeneration of phosphates, which are completely incorporated in the biological turnover, providing for phytoplankton development.
{"title":"Alkaline Phosphatase Activity and Phosphatase-Active Bacteria in Lake Baikal Water Column and Major Tributaries","authors":"M. Yu. Suslova, G. V. Podlesnaya, I. V. Tomberg, M. V. Sakirko, O. I. Belykh","doi":"10.1134/s0026261723603974","DOIUrl":"https://doi.org/10.1134/s0026261723603974","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>\u0000</h3><p>Phosphorus is one of the major biogenic elements. Its inflow facilitates eutrophication of lake water. In aquatic ecosystems, phosphorus is present mostly in organic compounds. Ability of aquatic microorganisms to assimilate phosphorus from organophosphorous compounds results from activity of alkaline phosphatases; activity of these enzymes may be an indicator of the state of the ecosystem, phosphate load, and water quality. In the present work, alkaline phosphatase activity (APA) and abundance of phosphatase-active bacteria (PAB) in Lake Baikal pelagic zone and in the mouths of its major tributaries was studied. In the pelagic zone, APA and PAB abundance decreased with depth, indicating that the main processes of phosphate generation occurred in the trophic layer of the lake. In the main tributaries, both APA and PAB abundance were considerably higher than in the pelagic zone. These results indicate active biochemical processes of transformation of organophosphorous compounds occur in the estuarine zones of the rivers. The degradation processes result in regeneration of phosphates, which are completely incorporated in the biological turnover, providing for phytoplankton development.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1134/s0026261723604207
G. L. Burygin, A. A. Khanina, M. V. Filippova
Abstract
O-polysaccharides of gram-negative bacteria are a highly variable component of the lipopolysaccharide molecules located at the cell wall surface and involved in microbial interaction with plant and animal cells. Activity of prophage genes often results in various non-stoichiometric modifications (methylation, acetylation, etc.) of glycans on the bacterial cell surface. The share of modified O-polysaccharides increases during the stationary growth phase and results in increased hydrophobicity of microbial surface. Bacterial cells with different hydrophobicity showed difference in attachment to plant roots. Increased cell hydrophobicity index was found to result in a significant increase in the number of adsorbed microorganisms per unit root length. Thus, acetyl transferase and methyl transferase genes of viral origin may be indirectly involved in successful colonization of plant roots by rhizosphere bacteria.
摘要革兰氏阴性细菌的O-多糖是位于细胞壁表面的脂多糖分子的一个高度可变成分，参与微生物与动植物细胞的相互作用。噬菌体基因的活性通常会导致细菌细胞表面的聚糖发生各种非均一修饰（甲基化、乙酰化等）。在静止生长阶段，修饰 O 型多糖的比例会增加，从而导致微生物表面的疏水性增加。不同疏水性的细菌细胞对植物根系的附着力也不同。研究发现，细胞疏水性指数增加会导致单位根长吸附的微生物数量显著增加。因此，病毒源乙酰转移酶和甲基转移酶基因可能间接参与了根瘤菌在植物根部的成功定殖。
{"title":"Effect of O-Polysaccharide Modifications on Successful Plant Colonization by Bacteria","authors":"G. L. Burygin, A. A. Khanina, M. V. Filippova","doi":"10.1134/s0026261723604207","DOIUrl":"https://doi.org/10.1134/s0026261723604207","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>\u0000</h3><p>O-polysaccharides of gram-negative bacteria are a highly variable component of the lipopolysaccharide molecules located at the cell wall surface and involved in microbial interaction with plant and animal cells. Activity of prophage genes often results in various non-stoichiometric modifications (methylation, acetylation, etc.) of glycans on the bacterial cell surface. The share of modified O-polysaccharides increases during the stationary growth phase and results in increased hydrophobicity of microbial surface. Bacterial cells with different hydrophobicity showed difference in attachment to plant roots. Increased cell hydrophobicity index was found to result in a significant increase in the number of adsorbed microorganisms per unit root length. Thus, acetyl transferase and methyl transferase genes of viral origin may be indirectly involved in successful colonization of plant roots by rhizosphere bacteria.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1134/s0026261723604098
W. Kurdy, G. Yu. Yakovleva, O. N. Ilyinskaya
Abstract
The studies of bacterial communities from extreme econiches are presently aimed mainly at analyzing the biodiversity of microorganisms using molecular biology methods. Cultivated bacteria from karst caves represent a unique group of microorganisms, the biochemical potential of which has been poorly studied. In the present work, bacteria from biofilms on the walls of the Kapova Cave (Shulgan-Tash Nature Reserve, Bashkortostan) were isolated and characterized in order to assess the ability of identified isolates to produce extracellular hydrolytic enzymes. Most of the isolates (89%) were members of the phylum Proteobacteria, with the remaining ones belonging to the phyla Actinobacteria, Firmicutes, and Bacteroidetes, which accounted for 5, 4, and 2% of the isolates, respectively. Strains with high levels of secreted protease, RNase, and amylase activity were identified as Stenotropomonas rhizophila, Lysinibacillus fusiformis, and Pseudomonas stutzeri, respectively.
{"title":"Biofilms from the Kapova Cave Walls as a Source of Hydrolase Producers","authors":"W. Kurdy, G. Yu. Yakovleva, O. N. Ilyinskaya","doi":"10.1134/s0026261723604098","DOIUrl":"https://doi.org/10.1134/s0026261723604098","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>\u0000</h3><p>The studies of bacterial communities from extreme econiches are presently aimed mainly at analyzing the biodiversity of microorganisms using molecular biology methods. Cultivated bacteria from karst caves represent a unique group of microorganisms, the biochemical potential of which has been poorly studied. In the present work, bacteria from biofilms on the walls of the Kapova Cave (Shulgan-Tash Nature Reserve, Bashkortostan) were isolated and characterized in order to assess the ability of identified isolates to produce extracellular hydrolytic enzymes. Most of the isolates (89%) were members of the phylum <i>Proteobacteria</i>, with the remaining ones belonging to the phyla <i>Actinobacteria</i>, <i>Firmicutes</i>, and <i>Bacteroidetes</i>, which accounted for 5, 4, and 2% of the isolates, respectively. Strains with high levels of secreted protease, RNase, and amylase activity were identified as <i>Stenotropomonas rhizophila</i>, <i>Lysinibacillus fusiformis</i>, and <i>Pseudomonas stutzeri</i>, respectively.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1134/s0026261723604232
T. Yu. Izmalkova, O. I. Sazonova, I. A. Kosheleva
Abstract
The effect of wastewater treatment plants in Pushchino, Russia, on abundance of antibiotic-resistant microorganisms and determinants of multiple drug resistance in the Oka River water and sediments was investigated. Treated wastewater was found to contribute to contamination of the Oka surface water layer with aminoglycoside-resistant strains. The role of Pushchino waste treatment plants in the dissemination of multidrug-resistant strains and plasmids of the A/C incompatibility groups (IncA/C) was demonstrated. Waste treatment plants were shown to affect both the total abundance of eubacteria in sediments and their composition, increasing the relative abundance of pseudomonads.
{"title":"Effect of Treated Wastewater on the Distribution of Antibiotic-Resistant Microorganisms in the Aquatic Environment","authors":"T. Yu. Izmalkova, O. I. Sazonova, I. A. Kosheleva","doi":"10.1134/s0026261723604232","DOIUrl":"https://doi.org/10.1134/s0026261723604232","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>\u0000</h3><p>The effect of wastewater treatment plants in Pushchino, Russia, on abundance of antibiotic-resistant microorganisms and determinants of multiple drug resistance in the Oka River water and sediments was investigated. Treated wastewater was found to contribute to contamination of the Oka surface water layer with aminoglycoside-resistant strains. The role of Pushchino waste treatment plants in the dissemination of multidrug-resistant strains and plasmids of the A/C incompatibility groups (IncA/C) was demonstrated. Waste treatment plants were shown to affect both the total abundance of eubacteria in sediments and their composition, increasing the relative abundance of pseudomonads.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1134/s0026261723603883
A. S. Aksenov, O. Ya. Kisil, A. S. Chervochkina, I. S. Khrebtova, K. S. Mantsurova, Yu. V. Bespalaya, O. V. Aksenova
Abstract
This is the first report on microbial communities associated with the freshwater snail Kamtschaticana kamtschatica inhabiting diverse types of water bodies in Eastern Siberia and the Russian Far East. Using metabarcoding data based on 16S rRNA gene fragments, taxonomic profiling of bacterial communities associated with snails from three water bodies of the Magadan Oblast was carried out. Predominant phyla in the studied bacterial communities were Pseudomonadota, Bacillota, Cyanobacteriota, Actinomycetota, Verrucomicrobiota, Planctomycetota, and Bacteroidota. The highest alpha-diversity, according to the Chao1 index, was revealed in the mollusks from the Orotukan Reservoir. The relative abundance of bacteria of the genera Snowella, Leptolyngbya, Nodosilinea, Arenimonas, and Polaromonas significantly distinguished the mollusks of this habitat from mollusks of the other two water bodies. The greatest similarities in the composition of the microbiota in K. kamtschatica were found for the genus Pseudomonas, which was present in the majority of samples in the amount of more than 1% and was not found in the sediment samples. Obtained data on the diversity and composition of bacterial communities associated with lymnaeid snails are of fundamental importance for the ecology of freshwater mollusks.
{"title":"Bacterial Communities within the Freshwater Lymnaeid Snail Kamtschaticana kamtschatica (Middendorff, 1850) in Northeastern Siberia","authors":"A. S. Aksenov, O. Ya. Kisil, A. S. Chervochkina, I. S. Khrebtova, K. S. Mantsurova, Yu. V. Bespalaya, O. V. Aksenova","doi":"10.1134/s0026261723603883","DOIUrl":"https://doi.org/10.1134/s0026261723603883","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>This is the first report on microbial communities associated with the freshwater snail <i>Kamtschaticana kamtschatica</i> inhabiting diverse types of water bodies in Eastern Siberia and the Russian Far East. Using metabarcoding data based on 16S rRNA gene fragments, taxonomic profiling of bacterial communities associated with snails from three water bodies of the Magadan Oblast was carried out. Predominant phyla in the studied bacterial communities were Pseudomonadota, Bacillota, Cyanobacteriota, Actinomycetota, Verrucomicrobiota, Planctomycetota, and Bacteroidota. The highest alpha-diversity, according to the Chao1 index, was revealed in the mollusks from the Orotukan Reservoir. The relative abundance of bacteria of the genera <i>Snowella</i>, <i>Leptolyngbya</i>, <i>Nodosilinea</i>, <i>Arenimonas</i>, and <i>Polaromonas</i> significantly distinguished the mollusks of this habitat from mollusks of the other two water bodies. The greatest similarities in the composition of the microbiota in <i>K. kamtschatica</i> were found for the genus <i>Pseudomonas</i>, which was present in the majority of samples in the amount of more than 1% and was not found in the sediment samples. Obtained data on the diversity and composition of bacterial communities associated with lymnaeid snails are of fundamental importance for the ecology of freshwater mollusks.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140563973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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