Pub Date : 2024-02-07DOI: 10.1134/s0026261723603469
A. V. Pelevina, Yu. Yu. Berestovskaya, A. G. Dorofeev, Yu. A. Nikolaev, E. V. Grouzdev, N. V. Pimenov, A. V. Mardanov
Abstract—
During long-term cultivation in a laboratory sequencing batch reactor in the phosphorus removal mode, granule-like aggregates of two morphotypes, differing in their physical structure and microbial composition, with predominance of different microbial physiological groups, were formed out of the activated sludge floccules. Phosphate-accumulating bacteria Candidatus “Accumulibacter” prevailed in one morphotype, and glycogen-accumulating bacteria Candidatus “Competibacter,” in the other. These are aerobic microorganisms, known to compete for the substrate during the anaerobic period of the anaerobic/aerobic cultivation cycle. Aggregate formation paralleled development of phosphate-accumulating microorganisms, increase in their relative abundance, and higher phosphorus removal efficiency.
{"title":"Aggregate Formation by a Microbial Community Developing in a Phosphorus-Removing Laboratory Reactor","authors":"A. V. Pelevina, Yu. Yu. Berestovskaya, A. G. Dorofeev, Yu. A. Nikolaev, E. V. Grouzdev, N. V. Pimenov, A. V. Mardanov","doi":"10.1134/s0026261723603469","DOIUrl":"https://doi.org/10.1134/s0026261723603469","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>During long-term cultivation in a laboratory sequencing batch reactor in the phosphorus removal mode, granule-like aggregates of two morphotypes, differing in their physical structure and microbial composition, with predominance of different microbial physiological groups, were formed out of the activated sludge floccules. Phosphate-accumulating bacteria <i>Candidatus</i> “Accumulibacter” prevailed in one morphotype, and glycogen-accumulating bacteria <i>Candidatus</i> “Competibacter,” in the other. These are aerobic microorganisms, known to compete for the substrate during the anaerobic period of the anaerobic/aerobic cultivation cycle. Aggregate formation paralleled development of phosphate-accumulating microorganisms, increase in their relative abundance, and higher phosphorus removal efficiency.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139761291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.1134/s0026261723603792
A. V. Hakobjanyan, R. E. Shcherbatov, T. A. Pankratov
Abstract
For the first time, phytopathogenic fungi of the genus Coleophoma were detected in the lichen genus Stereocaulon paschale (L.) Hoffm. On agarised nutrient medium, strain NM10F28209 formed compact, dense colonies with black ingrowing mycelium and ashy-grey aerial mycelium. Morphological characteristics and analysis of the ITS region of the gene cluster encoding ribosomal RNAs allowed us to assign the new strain to the genus Coleophoma; the closest relative was Coleophoma cylindrospora (Desm.) Höhn. (1919) with 98.28% of similarity. On prolonged incubation for 20 days, the culture formed a brown pigment that diffused into the agar. Extraction using a chloroform:acetone (1 : 1) mixture resulted in a multi-component preparation exhibiting fungicidal activity against yeasts from the Yarrowia and Candida genera. A compound belonging to the group of echinocandins, for which fungicidal activity has previously been demonstrated, was detected in the complex preparation by thin layer chromatography.
{"title":"A New Producer of Echinocandins, Ascomycete Coleophoma sp. Isolated from the Lichen Stereocaulon paschale","authors":"A. V. Hakobjanyan, R. E. Shcherbatov, T. A. Pankratov","doi":"10.1134/s0026261723603792","DOIUrl":"https://doi.org/10.1134/s0026261723603792","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>For the first time, phytopathogenic fungi of the genus <i>Coleophoma</i> were detected in the lichen genus <i>Stereocaulon paschale</i> (L.) Hoffm. On agarised nutrient medium, strain NM10F28209 formed compact, dense colonies with black ingrowing mycelium and ashy-grey aerial mycelium. Morphological characteristics and analysis of the ITS region of the gene cluster encoding ribosomal RNAs allowed us to assign the new strain to the genus <i>Coleophoma</i>; the closest relative was <i>Coleophoma cylindrospora</i> (Desm.) Höhn. (1919) with 98.28% of similarity. On prolonged incubation for 20 days, the culture formed a brown pigment that diffused into the agar. Extraction using a chloroform:acetone (1 : 1) mixture resulted in a multi-component preparation exhibiting fungicidal activity against yeasts from the <i>Yarrowia</i> and <i>Candida</i> genera. A compound belonging to the group of echinocandins, for which fungicidal activity has previously been demonstrated, was detected in the complex preparation by thin layer chromatography.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139761326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.1134/s0026261723603767
O. A. Galuza, N. E. Kovina, N. A. Korotkov, G. I. El-Registan, Yu. A. Nikolaev
Abstract—
It was shown that immobilization of lactic acid bacteria into organic gels (gelatin and gum) increased the titers of viable cells after 2 months of storage by 2.5–4 times in comparison to the control sample, and immobilization in gels of mixed composition (silanol-humate) led to their increase by 1–2 orders of magnitude. Storage of bacterial preparations in gels is promising for application in food industry and agrobiotechnologies.
{"title":"Long-Term Survival of Bacteria in Gels","authors":"O. A. Galuza, N. E. Kovina, N. A. Korotkov, G. I. El-Registan, Yu. A. Nikolaev","doi":"10.1134/s0026261723603767","DOIUrl":"https://doi.org/10.1134/s0026261723603767","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>It was shown that immobilization of lactic acid bacteria into organic gels (gelatin and gum) increased the titers of viable cells after 2 months of storage by 2.5–4 times in comparison to the control sample, and immobilization in gels of mixed composition (silanol-humate) led to their increase by 1–2 orders of magnitude. Storage of bacterial preparations in gels is promising for application in food industry and agrobiotechnologies.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139761348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.1134/s0026261723603573
A. A. Ivanova, S. N. Dedysh
Abstract
Bacteria of the phylum Verrucomicrobiota colonize a wide spectrum of terrestrial and aquatic habitats. One of the ecosystems where verrucomicrobia represent a significant part of the indigenous microbial community are wetlands. All earlier published information regarding phylogenetic diversity of verrucomicrobia in wetlands, however, refers to raised bogs, while verrucomicrobial diversity in fens remains poorly explored. In this study, we performed a comparative diversity analysis of Verrucomicrobiota in six fens of Vologda region. Nucleotide sequences of verrucomicrobia comprised 4 to 7% of all 16S rRNA gene reads retrieved from the examined peat samples. The most abundant groups were represented by members of the families Pedosphaeraceae, Opitutaceae and the order Chtoniobacterales, as well as the as-yet-uncultivated order-candidate S-BQ2-57. Numerical correlation between the relative abundance of well-represented taxa and peat characteristics was determined.
{"title":"Phylogenetic Diversity of Verrucomicrobiota in Fens of Northern Russia","authors":"A. A. Ivanova, S. N. Dedysh","doi":"10.1134/s0026261723603573","DOIUrl":"https://doi.org/10.1134/s0026261723603573","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Bacteria of the phylum <i>Verrucomicrobiota</i> colonize a wide spectrum of terrestrial and aquatic habitats. One of the ecosystems where verrucomicrobia represent a significant part of the indigenous microbial community are wetlands. All earlier published information regarding phylogenetic diversity of verrucomicrobia in wetlands, however, refers to raised bogs, while verrucomicrobial diversity in fens remains poorly explored. In this study, we performed a comparative diversity analysis of <i>Verrucomicrobiota</i> in six fens of Vologda region. Nucleotide sequences of verrucomicrobia comprised 4 to 7% of all 16S rRNA gene reads retrieved from the examined peat samples. The most abundant groups were represented by members of the families <i>Pedosphaeraceae</i>, <i>Opitutaceae</i> and the order <i>Chtoniobacterales</i>, as well as the as-yet-uncultivated order-candidate S-BQ2-57<i>.</i> Numerical correlation between the relative abundance of well-represented taxa and peat characteristics was determined.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139761369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.1134/s002626172360386x
M. Kh. Khasimov, E. P. Petushkova, O. P. Serdyuk, A. A. Tsygankov
Abstract—
The specific enzymatic reaction of sulfur reduction to hydrogen sulfide catalyzed by the HydSL hydrogenase of T. bogorovii BBS has so far been observed only in native cells. In this work, we were able to detect the hydrogen-dependent reaction H2 + S0 → H2S + HS– + H+ in a cell homogenate of these bacteria. This was achieved by destroying the cells in an optimized buffer of high ionic strength. Further purification of the preparation led to a 28-fold increase in its specific activity.
{"title":"Sulfur Metabolism in Thiocapsa bogorovii BBS and the Role of HydSL Hydrogenase","authors":"M. Kh. Khasimov, E. P. Petushkova, O. P. Serdyuk, A. A. Tsygankov","doi":"10.1134/s002626172360386x","DOIUrl":"https://doi.org/10.1134/s002626172360386x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>The specific enzymatic reaction of sulfur reduction to hydrogen sulfide catalyzed by the HydSL hydrogenase of <i>T. bogorovii</i> BBS has so far been observed only in native cells. In this work, we were able to detect the hydrogen-dependent reaction H<sub>2</sub> + S<sup>0</sup> → H<sub>2</sub>S + HS<sup>–</sup> + H<sup>+</sup> in a cell homogenate of these bacteria. This was achieved by destroying the cells in an optimized buffer of high ionic strength. Further purification of the preparation led to a 28-fold increase in its specific activity.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139761272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.1134/s0026261723603457
I. V. Mokrousov, S. N. Zhdanova, V. Valcheva, G. I. Alekseeva, M. K. Vinokurova, N. E. Evdokimova, O. B. Ogarkov
Abstract—
The S genotype (first described in Sicily, hence the name) is a part of the Euro-American lineage of Mycobacterium tuberculosis. Here, we report a method for its detection based on PCR-RFLP of the Rv0557 gene. The method was optimized on strains with known whole genome sequences and further applied to screening the DNA collections from Russia, Bulgaria, China, Japan, and Vietnam. PCR-RFLP analysis confirmed the S genotype in strains with the spoligotypes SIT34 (ancestral to S) and SIT1253 (Yakutia strains). Strains from Bulgaria with abridged spoligoprofiles SIT4 and SIT125 were also assigned to the S genotype. The developed method provides for rapid detection of the M. tuberculosis S genotype and reliably resolves phylogenetic ambiguity of the disputed spoligotypes.
摘要--S 基因型（首次描述于西西里岛，因此得名）是欧美结核分枝杆菌系的一部分。在此，我们报告了一种基于 Rv0557 基因 PCR-RFLP 的检测方法。该方法在已知全基因组序列的菌株上进行了优化，并进一步应用于筛选来自俄罗斯、保加利亚、中国、日本和越南的 DNA 收集物。PCR-RFLP 分析证实了具有 Spoligotypes SIT34（S 的祖先）和 SIT1253（雅库特菌株）的菌株的 S 基因型。来自保加利亚的菌株具有 SIT4 和 SIT125 的节略 spoligoprofiles，也被归入 S 基因型。所开发的方法可快速检测结核杆菌的 S 基因型，并可靠地解决有争议的 spoligotypes 系统发育模糊问题。
{"title":"Detection of the Mycobacterium tuberculosis S-Genotype and Clarification of the Position of Controversial Spoligotypes of the Euro-American Lineage","authors":"I. V. Mokrousov, S. N. Zhdanova, V. Valcheva, G. I. Alekseeva, M. K. Vinokurova, N. E. Evdokimova, O. B. Ogarkov","doi":"10.1134/s0026261723603457","DOIUrl":"https://doi.org/10.1134/s0026261723603457","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>The S genotype (first described in Sicily, hence the name) is a part of the Euro-American lineage of <i>Mycobacterium tuberculosis</i>. Here, we report a method for its detection based on PCR-RFLP of the <i>Rv0557</i> gene. The method was optimized on strains with known whole genome sequences and further applied to screening the DNA collections from Russia, Bulgaria, China, Japan, and Vietnam. PCR-RFLP analysis confirmed the S genotype in strains with the spoligotypes SIT34 (ancestral to S) and SIT1253 (Yakutia strains). Strains from Bulgaria with abridged spoligoprofiles SIT4 and SIT125 were also assigned to the S genotype. The developed method provides for rapid detection of the <i>M. tuberculosis</i> S genotype and reliably resolves phylogenetic ambiguity of the disputed spoligotypes.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139768492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.1134/s0026261723603639
A. R. Stroeva, A. D. Melnik, A. A. Klyukina, A. S. Pirogova, O. N. Vidishcheva, E. N. Poludetkina, G. G. Akhmanov, E. A. Bonch-Osmolovskaya, A. Yu. Merkel
Abstract—
High-performance sequencing of the 16S rRNA gene (V4 region) was used to reveal the most widespread groups of microorganisms in the bottom sediments of the Barents and Kara seas. In the Barents Sea, these were uncultured bacteria of the families Hyphomicrobiaceae, Anaerolineaceae, Desulfobulbaceae, and Desulfosarcinaceae (Sva0081 sediment group), actinobacteria of the genus Rhodococcus, and proteobacteria of the genus Woeseia. In the Kara Sea the most represented groups were uncultured bacteria of the order Sedimentisphaerales (the SG8-4 cluster, class Phycisphaerae, phylum Planсtomycetota), bacteria of the family Anaerolineaceae, the family Desulfosarcinaceae (the SEEP-SRB1 cluster), the deep phylogenetic cluster NB1-j, organotrophic proteobacteria of the genera Woeseia and Pseudomonas, and sulfate reducers of the genus Desulfatiglans. The patterns of microbial distribution were analyzed depending on the depth from the bottom surface and the presence of fluid discharge zones. It was found that according to analysis of the core microbiome, uncultured bacteria of the SG8-4 cluster and uncultured bacteria of the family Anaerolineaceae predominated in the methane discharge zones in the Barents and Kara seas.
{"title":"Microbial Community Structure in the Bottom Sediments of the Barents and Kara Seas and Their Relation to Methane Discharge","authors":"A. R. Stroeva, A. D. Melnik, A. A. Klyukina, A. S. Pirogova, O. N. Vidishcheva, E. N. Poludetkina, G. G. Akhmanov, E. A. Bonch-Osmolovskaya, A. Yu. Merkel","doi":"10.1134/s0026261723603639","DOIUrl":"https://doi.org/10.1134/s0026261723603639","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>High-performance sequencing of the 16S rRNA gene (V4 region) was used to reveal the most widespread groups of microorganisms in the bottom sediments of the Barents and Kara seas. In the Barents Sea, these were uncultured bacteria of the families <i>Hyphomicrobiaceae</i>, <i>Anaerolineaceae</i>, <i>Desulfobulbaceae</i>, and <i>Desulfosarcinaceae</i> (Sva0081 sediment group), actinobacteria of the genus <i>Rhodococcus</i>, and proteobacteria of the genus <i>Woeseia</i>. In the Kara Sea the most represented groups were uncultured bacteria of the order <i>Sedimentisphaerales</i> (the SG8-4 cluster, class <i>Phycisphaerae</i>, phylum <i>Planсtomycetota</i>), bacteria of the family <i>Anaerolineaceae</i>, the family <i>Desulfosarcinaceae</i> (the SEEP-SRB1 cluster), the deep phylogenetic cluster NB1-j, organotrophic proteobacteria of the genera <i>Woeseia</i> and <i>Pseudomonas</i>, and sulfate reducers of the genus <i>Desulfatiglans</i>. The patterns of microbial distribution were analyzed depending on the depth from the bottom surface and the presence of fluid discharge zones. It was found that according to analysis of the core microbiome, uncultured bacteria of the SG8-4 cluster and uncultured bacteria of the family <i>Anaerolineaceae</i> predominated in the methane discharge zones in the Barents and Kara seas.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140883816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.1134/s0026261723603925
A. G. Bulaev, A. V. Artykova, A. V. Kolosov, A. V. Nechaeva, A. V. Beletsky, V. V. Kadnikov, V. S. Melamud, A. V. Mardanov
Abstract—
The goal of the present work was to determine the effect of different concentrations of an organic carbon source (molasses) on the biooxidation of gold-bearing pyrite−arsenopyrite flotation concentrate, as well as on the composition of microbial populations that formed during the biooxidation process. Biooxidation activity increased with increasing molasses concentration in the medium. Concentration of molasses had a greater effect on the total number of microorganisms, while the relative numbers of microorganisms of different groups did not differ significantly. Thus, it was shown that the use of cheap organic substrates is promising to increase the efficiency of biohydrometallurgical processes.
{"title":"Influence of Organic Carbon Source on Biooxidation of Sulfide Concentrate","authors":"A. G. Bulaev, A. V. Artykova, A. V. Kolosov, A. V. Nechaeva, A. V. Beletsky, V. V. Kadnikov, V. S. Melamud, A. V. Mardanov","doi":"10.1134/s0026261723603925","DOIUrl":"https://doi.org/10.1134/s0026261723603925","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>The goal of the present work was to determine the effect of different concentrations of an organic carbon source (molasses) on the biooxidation of gold-bearing pyrite−arsenopyrite flotation concentrate, as well as on the composition of microbial populations that formed during the biooxidation process. Biooxidation activity increased with increasing molasses concentration in the medium. Concentration of molasses had a greater effect on the total number of microorganisms, while the relative numbers of microorganisms of different groups did not differ significantly. Thus, it was shown that the use of cheap organic substrates is promising to increase the efficiency of biohydrometallurgical processes.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140883997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.1134/s002626172360369x
D. V. Badmadashiev, A. R. Stroeva, A. A. Klyukina, E. N. Poludetkina, E. A. Bonch-Osmolovskaya
Abstract—
Investigation of prokaryotic communities from different horizons of the bottom sediments in the Kandalaksha Gulf, White Sea revealed two characteristic groups of sampling points. The first group demonstrated stratification of prokaryotic communities depending on the horizon depth, while the second one, with uniform prokaryotic communities, was typical of the sections with active organic matter decomposition. Microorganisms involved in decomposition of labile organic compounds (Woeseia and Sandaracinaceae), as well as sulfate reducers (SEEP-SRB1 and Sva0081) predominated in the upper horizons of the stratified sediments (the first group of samples). In the lower layers (30 and 50 cm), the share of microorganisms potentially involved in the degradation of complex compounds (e.g., Desulfatiglans, Hyphomicrobiaceae, and Mycobacterium) increased. The share of prokaryotes with unknown metabolism, such as JS1, SG8-4, WCHB1-81, Aerophobales, and S085, increased as well. Thus, the structure of prokaryotic communities was affected by the presence and abundance of organic matter, which decreased with depth.
{"title":"Stratification of Prokaryotic Communities in the White Sea Bottom Sediments","authors":"D. V. Badmadashiev, A. R. Stroeva, A. A. Klyukina, E. N. Poludetkina, E. A. Bonch-Osmolovskaya","doi":"10.1134/s002626172360369x","DOIUrl":"https://doi.org/10.1134/s002626172360369x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>Investigation of prokaryotic communities from different horizons of the bottom sediments in the Kandalaksha Gulf, White Sea revealed two characteristic groups of sampling points. The first group demonstrated stratification of prokaryotic communities depending on the horizon depth, while the second one, with uniform prokaryotic communities, was typical of the sections with active organic matter decomposition. Microorganisms involved in decomposition of labile organic compounds (<i>Woeseia</i> and <i>Sandaracinaceae</i>), as well as sulfate reducers (SEEP-SRB1 and Sva0081) predominated in the upper horizons of the stratified sediments (the first group of samples). In the lower layers (30 and 50 cm), the share of microorganisms potentially involved in the degradation of complex compounds (e.g., <i>Desulfatiglans</i>, <i>Hyphomicrobiaceae</i>, and <i>Mycobacterium</i>) increased. The share of prokaryotes with unknown metabolism, such as JS1, SG8-4, WCHB1-81, <i>Aerophobales</i>, and S085, increased as well. Thus, the structure of prokaryotic communities was affected by the presence and abundance of organic matter, which decreased with depth.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139761266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.1134/s002626172360338x
M. A. Kupryashina, E. P. Vetchinkina, A. S. Abdrakhmanova, E. G. Ponomareva, T. E. Pylaev, V. A. Khanadeev, A. M. Burov
Abstract—
Herein we present the results of optimization of silver nanoparticles biosynthesis using a nonpathogenic soil bacterium Azospirillum brasilense. The conditions and parameters for synthesis of biogenic nanoparticles using A. brasilense vegetative cells, culture liquid, and homogeneous polyphenol oxidase preparations were analyzed. We have revealed the ranging effect of the incubation time, the concentration of silver ions, pH of the reaction mixture, and the concentration of metabolites, towards the production of colloidal silver nanoparticles with specified geometric parameters, high colloidal stability and relatively good monodispersity. The size, shape, and relative yield of electron-dense nanostructures were determined by transmission electron microscopy and dynamic light scattering; extinction spectra were recorded by UV-vis spectrophotometry.
{"title":"Optimization of Bioformation of Silver Nanoparticles Using Azospirillum brasilense Cultures","authors":"M. A. Kupryashina, E. P. Vetchinkina, A. S. Abdrakhmanova, E. G. Ponomareva, T. E. Pylaev, V. A. Khanadeev, A. M. Burov","doi":"10.1134/s002626172360338x","DOIUrl":"https://doi.org/10.1134/s002626172360338x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000<b>Abstract</b>—</h3><p>Herein we present the results of optimization of silver nanoparticles biosynthesis using a nonpathogenic soil bacterium <i>Azospirillum brasilense</i>. The conditions and parameters for synthesis of biogenic nanoparticles using <i>A. brasilense</i> vegetative cells, culture liquid, and homogeneous polyphenol oxidase preparations were analyzed. We have revealed the ranging effect of the incubation time, the concentration of silver ions, pH of the reaction mixture, and the concentration of metabolites, towards the production of colloidal silver nanoparticles with specified geometric parameters, high colloidal stability and relatively good monodispersity. The size, shape, and relative yield of electron-dense nanostructures were determined by transmission electron microscopy and dynamic light scattering; extinction spectra were recorded by UV-vis spectrophotometry.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139761604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号