R. Rosariastuti, S. Sumani, S. Supriyadi, Muhammad Ardian Nursetyawan, Pramusita Yoga Daniswara
Fertilizer demand in Indonesia increase for increasing agricultural production.Biofertilizer is organic fertilizer with the addition of specific microorganisms which are expected to reduce the amount of inorganic fertilizer. Carrier material plays an important role in maintaining the viability and storage period. Peat is used as a biofertilizer carrier for a long time. Solid waste of Modified Cassava Flour (Mocaf) which is the remainder of mocaf industry has great potential as a carrier material of good biofertilizers, because of its nutrient content. The aim of this study was determining the potential of mocaf solid waste and its combinations with peat as the carrier in supporting the growth of Nitrogen-fixing Bacteria (NFB) and Phosphate Solubilizing Bacteria (PSB) during the incubation of microorganisms. The experiment was conducted at the Laboratory of Soil Biology and Biotechnology, Faculty of Agriculture of Sebelas Maret University (UNS) using completely randomized design (CRD) with two factors of a carrier and incubation time as the experimental design. There were three types of carrier which have different combination. The base material were solid waste of Mocaf industry and peat. All materials of carrier were mixed and sterilized, than inoculated by Nitrogen- Fixing Bacteria (NFB) and Phosphate Solubilizing Bacteria (PSB) and incubated for 60 days. The growth of bacterias were analyzed every 15 days and the chemical composition of carrier were analyzed at the begin and the end of research (incubation). The results indicated that the incubation time significantlyaffected viability of NFB and PSB. Until 60th day incubation time, still showed the increasing growth of NFB and PSB.
{"title":"The Utilization of Modified Cassava Flour (Mocaf) Industry Waste and Peat as Carrier of Nitrogen-Fixing Bacteria and Phosphate Solubilizing Bacteria Inoculant","authors":"R. Rosariastuti, S. Sumani, S. Supriyadi, Muhammad Ardian Nursetyawan, Pramusita Yoga Daniswara","doi":"10.5454/MI.11.4.%P","DOIUrl":"https://doi.org/10.5454/MI.11.4.%P","url":null,"abstract":"Fertilizer demand in Indonesia increase for increasing agricultural production.Biofertilizer is organic fertilizer with the addition of specific microorganisms which are expected to reduce the amount of inorganic fertilizer. Carrier material plays an important role in maintaining the viability and storage period. Peat is used as a biofertilizer carrier for a long time. Solid waste of Modified Cassava Flour (Mocaf) which is the remainder of mocaf industry has great potential as a carrier material of good biofertilizers, because of its nutrient content. The aim of this study was determining the potential of mocaf solid waste and its combinations with peat as the carrier in supporting the growth of Nitrogen-fixing Bacteria (NFB) and Phosphate Solubilizing Bacteria (PSB) during the incubation of microorganisms. The experiment was conducted at the Laboratory of Soil Biology and Biotechnology, Faculty of Agriculture of Sebelas Maret University (UNS) using completely randomized design (CRD) with two factors of a carrier and incubation time as the experimental design. There were three types of carrier which have different combination. The base material were solid waste of Mocaf industry and peat. All materials of carrier were mixed and sterilized, than inoculated by Nitrogen- Fixing Bacteria (NFB) and Phosphate Solubilizing Bacteria (PSB) and incubated for 60 days. The growth of bacterias were analyzed every 15 days and the chemical composition of carrier were analyzed at the begin and the end of research (incubation). The results indicated that the incubation time significantlyaffected viability of NFB and PSB. Until 60th day incubation time, still showed the increasing growth of NFB and PSB.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"29 1","pages":"111-116"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73916820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Indole-3-acetic acid (IAA) is the main member of the auxin family that controls many important physiological processes in plant. Such beneficial IAA that produced by plant growth promoting rhizobacteria (PGPR), enhances plant growth and was believed to increase the access to more nutrients in the soil. The precursor for syntetizing IAA is tryptophan, and it was also found in the sprout or other sources of protein. The aim of this study was to investigate the best bacteria and growth medium supplemented with extract of bean sprout or fish meal as the sources of precursor for the IAA production. Several bacterial isolates were screened for highest IAA production. IAA production was measured with High Performance Liquid Chromatography. All of isolates were able to produce IAA and isolates PS1 was selected for the further assay by cultivating under fermentor system. Sequencing of 16S rDNA of PS1 isolate indicated as Acinetobacter sp. The result showed that the highest IAA production during fermetation was 62,428 ppm found in under medium supplemented with mung bean sprout extracts grown in fermentor, after 24 hours incubation.
{"title":"The use of Sprout as Precursor for the Production of Indole Acetic Acid by Selected Plant Growth Promoting Rhizobacteria Grown in the Fermentor","authors":"S. Antonius, Rachel Budisatria, T. K. Dewi","doi":"10.5454/MI.10.4.3","DOIUrl":"https://doi.org/10.5454/MI.10.4.3","url":null,"abstract":"Indole-3-acetic acid (IAA) is the main member of the auxin family that controls many important physiological processes in plant. Such beneficial IAA that produced by plant growth promoting rhizobacteria (PGPR), enhances plant growth and was believed to increase the access to more nutrients in the soil. The precursor for syntetizing IAA is tryptophan, and it was also found in the sprout or other sources of protein. The aim of this study was to investigate the best bacteria and growth medium supplemented with extract of bean sprout or fish meal as the sources of precursor for the IAA production. Several bacterial isolates were screened for highest IAA production. IAA production was measured with High Performance Liquid Chromatography. All of isolates were able to produce IAA and isolates PS1 was selected for the further assay by cultivating under fermentor system. Sequencing of 16S rDNA of PS1 isolate indicated as Acinetobacter sp. The result showed that the highest IAA production during fermetation was 62,428 ppm found in under medium supplemented with mung bean sprout extracts grown in fermentor, after 24 hours incubation.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"0 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73449542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christian Heryakusuma, Fernita Puspasari, Ihsanawati Ihsanawati, E. Giri-Rachman, M. Tan, Eka Ramadhani, N. Nurainy, D. Natalia
Recombinant small hepatitis B surface antigen (sHBsAg) is used as a vaccine component to prevent hepatitis B virus infection. As an attempt to produce local recombinant sHBsAg, a PCR-amplified DNA fragment encoding Indonesia sHBsAg which belongs to B genotype and adw2 subtype was cloned into Hansenula polymorpha expression vector pHIPX4 by using recombination method. The resulted pHIPX4-sHBsAg was integrated into the alcohol oxidase (AOX) locus of H. polymorpha NCYC495 genome and the sHBsAg expression was regulated under the control of H. polymorpha AOX promoter. H. polymorpha NCYC495 carrying the sHBsAg coding sequence was grown in mineral medium and methanol 0.5% (v/v) was added to induce the expression of recombinant sHBsAg. The expression of sHBsAg was detected by HBsAg diagnostic kit test, ELISA, and Western blot analysis.
{"title":"Cloning and Expression of Small Hepatitis B Surface Antigen (sHBsAg) In Hansenula polymorpha","authors":"Christian Heryakusuma, Fernita Puspasari, Ihsanawati Ihsanawati, E. Giri-Rachman, M. Tan, Eka Ramadhani, N. Nurainy, D. Natalia","doi":"10.5454/MI.10.4.1","DOIUrl":"https://doi.org/10.5454/MI.10.4.1","url":null,"abstract":"Recombinant small hepatitis B surface antigen (sHBsAg) is used as a vaccine component to prevent hepatitis B virus infection. As an attempt to produce local recombinant sHBsAg, a PCR-amplified DNA fragment encoding Indonesia sHBsAg which belongs to B genotype and adw2 subtype was cloned into Hansenula polymorpha expression vector pHIPX4 by using recombination method. The resulted pHIPX4-sHBsAg was integrated into the alcohol oxidase (AOX) locus of H. polymorpha NCYC495 genome and the sHBsAg expression was regulated under the control of H. polymorpha AOX promoter. H. polymorpha NCYC495 carrying the sHBsAg coding sequence was grown in mineral medium and methanol 0.5% (v/v) was added to induce the expression of recombinant sHBsAg. The expression of sHBsAg was detected by HBsAg diagnostic kit test, ELISA, and Western blot analysis.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"33 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91399215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Z. Mustopa, Hidayah Murtiyaningsih, F. Fatimah, S. Suharsono
Plantaricin F (pln F) is bacteriocins produced by Lactobacillus plantarum are mostly applied in food to prevent microbial contamination. Biosynthesis of pln F is controlled by plantaricin A (pln A) which is primarily a peptide pheromone that controls the production of antimicrobial peptides in L. plantarum. Pre-mature pln A contains signal peptide and utilizes the general secretory pathway for export this peptide. The aim of this study was to construct a fusion of pln A signal peptide with mature pln F and to investigate the antimicrobial activity of pln F. Extracellular pln A- encoding the plnA gene were cloned into pGEM-Teasy vector to be used as a source for signal peptide SP pln A. A polymerase chain reaction (PCR) overlaps technique has been used in the construction of the fused gene with size of 171 bp while the individual gene obtained by this technique was 66 bp for pln A signal peptide and 105 bp for pln F. A gene encoding the pln A signal peptide (SP pln A) fused to mature plantaricin F, fused gene were then cloned into pNZ8148 as expression vector under the control of the nisin promoter (Pnis A) to generate a pNZ8148 SP pln A-plnF. Molecular expression study showed that recombinant Lactococcus lactis NZ3900 was able to express the mature pln F at transcription and translation level with size of 171 bp (by RT-PCR) and 3.8 kDa (by SDS-PAGE), respectively after 0.5-5 ng/ml nisin induction (OD 600 0,5). Furthermore, the supernatants of the recombinant L. lactis NZ3900 showed antimicrobial activity against Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6539 and Listeria monocytogenes BTCC B693. Collectively, the successfulness of expression of functional pln F gene under the control of nisin induction in L. lactis NZ3900, for the first time.
{"title":"Cloning and Heterologous Expression of Extracellular Plantaricin F Produced by Lactobacillus plantarum S34 Isolated from “Bekasam” in Lactococcus lactis","authors":"A. Z. Mustopa, Hidayah Murtiyaningsih, F. Fatimah, S. Suharsono","doi":"10.5454/MI.10.3.3","DOIUrl":"https://doi.org/10.5454/MI.10.3.3","url":null,"abstract":"Plantaricin F (pln F) is bacteriocins produced by Lactobacillus plantarum are mostly applied in food to prevent microbial contamination. Biosynthesis of pln F is controlled by plantaricin A (pln A) which is primarily a peptide pheromone that controls the production of antimicrobial peptides in L. plantarum. Pre-mature pln A contains signal peptide and utilizes the general secretory pathway for export this peptide. The aim of this study was to construct a fusion of pln A signal peptide with mature pln F and to investigate the antimicrobial activity of pln F. Extracellular pln A- encoding the plnA gene were cloned into pGEM-Teasy vector to be used as a source for signal peptide SP pln A. A polymerase chain reaction (PCR) overlaps technique has been used in the construction of the fused gene with size of 171 bp while the individual gene obtained by this technique was 66 bp for pln A signal peptide and 105 bp for pln F. A gene encoding the pln A signal peptide (SP pln A) fused to mature plantaricin F, fused gene were then cloned into pNZ8148 as expression vector under the control of the nisin promoter (Pnis A) to generate a pNZ8148 SP pln A-plnF. Molecular expression study showed that recombinant Lactococcus lactis NZ3900 was able to express the mature pln F at transcription and translation level with size of 171 bp (by RT-PCR) and 3.8 kDa (by SDS-PAGE), respectively after 0.5-5 ng/ml nisin induction (OD 600 0,5). Furthermore, the supernatants of the recombinant L. lactis NZ3900 showed antimicrobial activity against Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6539 and Listeria monocytogenes BTCC B693. Collectively, the successfulness of expression of functional pln F gene under the control of nisin induction in L. lactis NZ3900, for the first time.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"78 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2016-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86233514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bacterial biofilms produced by pathogenic bacteria have become a serious issue in several chronic diseases such as atherosclerosis, cystic fibrosis, endocarditis, inner ear infections, and kidney stones. Thus, inhibition and destruction of bacterial biofilm from pathogenic bacteria is needed. The purpose of this study is to analyze biofilm inhibition and destruction activities of marine bacteria associated with hard and soft corals isolated from several oceanic regions in Indonesia. Fifteen marine isolates collected from several regions in Indonesia such as Bali Province, South East Sulawesi Province, East Java Province, Lampung Province, and Banten Province were tested using static biofilm assay against several pathogenic bacteria. Biofilm of the pathogenic bacteria tested were stained using 0.4% crystal violet. Several isolates were sequenced using 16S rRNA PCR method. Most of marine isolates presented higher inhibition and or destruction activity at 10% crude concentration. Few isolates were further identified using 16S rRNA and proven to have antibiofilm activity against several pathogenic bacteria. Marine bacteria have broad applications in medical and pharmaceutical industries and the oceanic regions of Indonesia are promising sources for the discovery of novel bacteria with antibiofilm activity.
{"title":"Screening of Antibiofilm Activity from Marine Bacteria against Pathogenic Bacteria","authors":"A. Camesi, A. Lukito, D. Waturangi, H. Kwan","doi":"10.5454/MI.10.3.2","DOIUrl":"https://doi.org/10.5454/MI.10.3.2","url":null,"abstract":"Bacterial biofilms produced by pathogenic bacteria have become a serious issue in several chronic diseases such as atherosclerosis, cystic fibrosis, endocarditis, inner ear infections, and kidney stones. Thus, inhibition and destruction of bacterial biofilm from pathogenic bacteria is needed. The purpose of this study is to analyze biofilm inhibition and destruction activities of marine bacteria associated with hard and soft corals isolated from several oceanic regions in Indonesia. Fifteen marine isolates collected from several regions in Indonesia such as Bali Province, South East Sulawesi Province, East Java Province, Lampung Province, and Banten Province were tested using static biofilm assay against several pathogenic bacteria. Biofilm of the pathogenic bacteria tested were stained using 0.4% crystal violet. Several isolates were sequenced using 16S rRNA PCR method. Most of marine isolates presented higher inhibition and or destruction activity at 10% crude concentration. Few isolates were further identified using 16S rRNA and proven to have antibiofilm activity against several pathogenic bacteria. Marine bacteria have broad applications in medical and pharmaceutical industries and the oceanic regions of Indonesia are promising sources for the discovery of novel bacteria with antibiofilm activity.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"428 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2016-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73519758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This research was carried out by developing the carbon capture and storage (CCS) technology to determine the synergism between microalgae consortium and the optimum nutrient ratio as an effort to obtain higher CO 2 removal efficiency and CO 2 utilization efficiency. The microalgae consortium consisting of Chlorella sp., Scenedesmus sp. and Ankistrodesmus sp. have been selected previously as potential candidates for Microbial Carbon Capture and Storage (MCCS) agent and already cultured continuously in PHM (Provasoli Haematococcus Media) artificial medium, in vertical column photobioreactor. Pure CO 2 gas at a high concentration of 10% (v/v) flowed from the bottom of vertical column photobioreactor continuously with optimum flow rate of 5 L.min -1 . A growth medium (PHM) containing artificial nutrients was flowed continuously at flow rate 7L.day -1 and detention time 3.8 days. Four fluorescent lamps were positioned outside the photo-bioreactor to obtain light intensity of 4000 lux, set for 16 hours light exposure and 8 hours dark, with operating temperature 30°C maintained during the study. Three compositional variations of microalgae consortium were used. They are as follows; Ch : Sc : An = 1: 1: 1; Ch : Sc = 1: 1; and Ch : An = 1: 1, where Ch, Sc, and An were Chlorella sp., Scenedesmus obliquus and Ankistrodesmus sp., respectively. The following variations of nutrient composition were used; C: N: P = 100: 10: 1, C: N: P = 100: 50: 1 and C: N: P = 100: 25: 1. The C, N, and P sources were CO 2 (inorganic), KNO 3 , and KH 2 PO 4 , respectively. This study proves that synergism between the types making up the consortium also determined the ability to utilize inorganic carbon source. Without the presence of Ankistrodesmus sp., synergism between Scenedesmus obliquus and Chlorella sp. showed twice higher CO 2 utilization efficiency in comparison to the synergism between Ankistrodesmus sp and Chlorella sp. Increased nitrogen concentration in medium increased the growth of Chlorella sp and Scenedesmus obliquus as a consortium, the CO 2 removal efficiency, the CO 2 utilization efficiency and the Carbon Uptake Rate. The nutrient ratios C:N:P of 100:50:1 could increase CO 2 utilization efficiency upto 50% higher than the C:N:P of 100:10:1.
本研究通过开发碳捕集与封存(CCS)技术，确定微藻联合体与最佳营养配比之间的协同作用，以获得更高的CO 2去除效率和CO 2利用效率。由小球藻(Chlorella sp.)、Scenedesmus sp.和Ankistrodesmus sp.组成的微藻联盟已被选为微生物碳捕获和储存(MCCS)剂的潜在候选物，并已在PHM (Provasoli Haematococcus Media)人工培养基中在垂直柱光生物反应器中进行了连续培养。高浓度10% (v/v)的纯co2气体从垂直塔式光生物反应器底部连续流出，最佳流量为5 L.min -1。将含人工营养物的生长培养基(PHM)以7L的流速连续流动。第1天，留校时间3.8天。在光生物反应器外放置4支荧光灯，光照强度为4000 lux，光照16小时，黑暗8小时，研究期间工作温度为30°C。采用三种微藻群落组成变化。它们是:Ch: Sc: An = 1:1:1;Ch: Sc = 1:1;和Ch: An = 1:1，其中Ch、Sc和An分别代表小球藻、斜角小球藻和按目小球藻。采用了以下几种营养成分:C: N: P = 100:10:1, C: N: P = 100:50:1, C: N: P = 100:25:1。碳、氮和磷的来源分别是co2(无机)、kno3和kpo4。本研究证明，组成联合体的类型之间的协同作用也决定了利用无机碳源的能力。在无安氏线虫存在的情况下，斜小球藻与小球藻的协同作用对CO 2的利用效率比安氏线虫与小球藻的协同作用高2倍。培养基中氮浓度的增加提高了小球藻与斜小球藻作为一个群落的生长、CO 2的去除效率、CO 2的利用效率和碳吸收率。养分比C:N:P为100:50:1比C:N:P为100:10:1可提高co2利用效率达50%。
{"title":"Enhancing the Removal of Highly Concentrated CO2 Through Synergism between Microalgae Consortium and Nutrient Ratio in Photobioreactor","authors":"A. Rinanti, K. Dewi","doi":"10.5454/MI.10.3.1","DOIUrl":"https://doi.org/10.5454/MI.10.3.1","url":null,"abstract":"This research was carried out by developing the carbon capture and storage (CCS) technology to determine the synergism between microalgae consortium and the optimum nutrient ratio as an effort to obtain higher CO 2 removal efficiency and CO 2 utilization efficiency. The microalgae consortium consisting of Chlorella sp., Scenedesmus sp. and Ankistrodesmus sp. have been selected previously as potential candidates for Microbial Carbon Capture and Storage (MCCS) agent and already cultured continuously in PHM (Provasoli Haematococcus Media) artificial medium, in vertical column photobioreactor. Pure CO 2 gas at a high concentration of 10% (v/v) flowed from the bottom of vertical column photobioreactor continuously with optimum flow rate of 5 L.min -1 . A growth medium (PHM) containing artificial nutrients was flowed continuously at flow rate 7L.day -1 and detention time 3.8 days. Four fluorescent lamps were positioned outside the photo-bioreactor to obtain light intensity of 4000 lux, set for 16 hours light exposure and 8 hours dark, with operating temperature 30°C maintained during the study. Three compositional variations of microalgae consortium were used. They are as follows; Ch : Sc : An = 1: 1: 1; Ch : Sc = 1: 1; and Ch : An = 1: 1, where Ch, Sc, and An were Chlorella sp., Scenedesmus obliquus and Ankistrodesmus sp., respectively. The following variations of nutrient composition were used; C: N: P = 100: 10: 1, C: N: P = 100: 50: 1 and C: N: P = 100: 25: 1. The C, N, and P sources were CO 2 (inorganic), KNO 3 , and KH 2 PO 4 , respectively. This study proves that synergism between the types making up the consortium also determined the ability to utilize inorganic carbon source. Without the presence of Ankistrodesmus sp., synergism between Scenedesmus obliquus and Chlorella sp. showed twice higher CO 2 utilization efficiency in comparison to the synergism between Ankistrodesmus sp and Chlorella sp. Increased nitrogen concentration in medium increased the growth of Chlorella sp and Scenedesmus obliquus as a consortium, the CO 2 removal efficiency, the CO 2 utilization efficiency and the Carbon Uptake Rate. The nutrient ratios C:N:P of 100:50:1 could increase CO 2 utilization efficiency upto 50% higher than the C:N:P of 100:10:1.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"10 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2016-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90664549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. A. Rifqi, Clara M. Koesharto, I. Surono, S. Marliyati
The aim of the research was to study the effect of functional feed of catfish’ flour, oil and probiotic E. faecium IS-27526 based on bodyweight and CRP (C-reactive protein) of aged female Cynomolgus monkey ( Macaca fascicularis ). Nine aged female Cynolmolgus randomly divided into three groups. The age is determined by dentition, with bodyweight in a range of 2 – 4 kg. Animals were placed in individual cages in the position where they can interact audiovisually. Feed composition consists of sugar, egg, soy flour, wheat flour, sweet potato flour, butter, egg yolk flour, catfish’ flour and oil, and microencapsulated probiotic Enterococcus faecium IS-27526 and administered for 90 days. Evaluation of bodyweight (BW) and CRP were conducted. There is no significant effect (p>0.05) of experimental diets on bodyweight in each group. However, probiotic tends to delay the bodyweight gain. The bodyweight of cynomolgus in probiotic diet is shown more stable than others. There is no the effect of experimental diets on CRP which is marked by negative result of CRP test. Probiotic E. faecium IS-27526 is potential for bodyweight homeostasis regulation to reduce the risk of overweight and obesity.
{"title":"Effect of catfish’ (Clarias gariepinus) flour and oil with probiotic Enterococcus faecium IS-27526 based functional feed provision on bodyweight and C-reactive protein (CRP) of aged atherogenic female Cynomolgus monkey (Macaca fascicularis)","authors":"M. A. Rifqi, Clara M. Koesharto, I. Surono, S. Marliyati","doi":"10.5454/MI.10.4.5","DOIUrl":"https://doi.org/10.5454/MI.10.4.5","url":null,"abstract":"The aim of the research was to study the effect of functional feed of catfish’ flour, oil and probiotic E. faecium IS-27526 based on bodyweight and CRP (C-reactive protein) of aged female Cynomolgus monkey ( Macaca fascicularis ). Nine aged female Cynolmolgus randomly divided into three groups. The age is determined by dentition, with bodyweight in a range of 2 – 4 kg. Animals were placed in individual cages in the position where they can interact audiovisually. Feed composition consists of sugar, egg, soy flour, wheat flour, sweet potato flour, butter, egg yolk flour, catfish’ flour and oil, and microencapsulated probiotic Enterococcus faecium IS-27526 and administered for 90 days. Evaluation of bodyweight (BW) and CRP were conducted. There is no significant effect (p>0.05) of experimental diets on bodyweight in each group. However, probiotic tends to delay the bodyweight gain. The bodyweight of cynomolgus in probiotic diet is shown more stable than others. There is no the effect of experimental diets on CRP which is marked by negative result of CRP test. Probiotic E. faecium IS-27526 is potential for bodyweight homeostasis regulation to reduce the risk of overweight and obesity.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"20 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83277595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Hindersah, P. Priyanka, Wilhelmina Rumahlewang, A. Kalay
Leafy vegetables contributes to the inflation rate in Ambon City due to low productivity in rainy season. Some vegetables are imported from other islands while importantvegetables such as local petsai (Brassica chinensis L.) and chili (Capsicum annum L.) are cultivated in low nitrogen soil, Entisols. Lack of nitrogen could be overcome by using inorganic fertilizeras well as biofertilzer. The soil can be inoculated with rhizobacteria, such as Azotobacter, to increase the nitrogen uptake and improve the quality of vegetables. This research was conducted to isolate and select Azotobacter from rhizosphere of vegetables and to examine the effect of Azotobacter inoculation on chili-seedling growth and nitrogen uptake by using bioassay method. Azotobacter sp. was isolated in nitrogen-free Ashby’s Media. The bioassay was held in the green house with randomized block design experiment, which examined the combination of isolates and population of Azotobacter sp. on chili. Two best isolates which was selected based on pH, nitrogen content and cell viability were s2a10 (from petsai's rhizosphere) and c2a9 (from chili’s rhizosphere). Bioassay showed that Azotobacter inoculation followed by reduced NPK fertilizer doses had no effect on transplant dry weight and nitrogen uptake. All Azotobacter 8 -1inoculation except 10 CFU mL s2a10 maintain soil nitrogen although Azotobacter population in soil was slightly reduced. This showed that Azotobacter sp. potentially reduce the use of inorganic biofertilizer.
由于雨季的低生产力，叶菜类蔬菜对安汶市的通货膨胀率有贡献。一些蔬菜是从其他岛屿进口的，而一些重要的蔬菜，如当地的芸苔(Brassica chinensis L.)和辣椒(Capsicum annum L.)则在低氮土壤(Entisols)上种植。缺氮可以通过使用无机肥料和生物肥料来克服。可在土壤中接种固氮菌等根瘤菌，增加氮素吸收量，改善蔬菜品质。本研究从蔬菜根际分离和筛选了固氮菌，并采用生物测定法研究了接种固氮菌对辣椒幼苗生长和氮素吸收的影响。在无氮Ashby培养基中分离到固氮细菌。采用随机区组设计试验，对辣椒上固氮菌的分离和种群组合进行了生物测定。根据pH值、含氮量和细胞活力筛选出的最佳分离菌株分别为petsai根际菌株s2a10和辣椒根际菌株c2a9。生物试验表明，接种固氮菌后减少氮磷钾用量对移栽干重和氮素吸收没有影响。除10 CFU mL s2a10外，接种8 -1固氮菌均能维持土壤氮素，但土壤中固氮菌数量略有减少。这表明，固氮菌可能会减少无机生物肥料的使用。
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Lipase is a lipids hydrolyze enzyme which are widely used in various industries such as chemical, pharmaceutical, food industries, and detergents. Bacillus licheniformis F11.4 is one of the bacteria with potential source of lipase. This study aimed to obtain optimum production of lipase from B. licheniformis F11.4 by optimizing the composition of media and pH values with fish flour as a replacement for peptone and yeast extract based medium. Selection of the significant factors used a 2-level factorial design. The upper limit and lower limit of the selected factors was optimized using Central Composite Design (CCD) and the data analysis was performed using the Response Surface Methodology (RSM). Fermentation was carried out in erlenmeyer at initial pH 8 and a temperature of 37 °C, using a shaker incubator at 150 rpm. A fermentation system for lipases production is considered optimal when its desirability value closes to 1. By using numerical optimization, an optimal medium could be obtained, i.e. consisting of OO:CPO 0.14 % (w/v) and fish flour 2% (w/v), at pH 8 and 150 rpm, which produced lipase with enzyme activity of 1.563 U mL-1 and protein level of 0.08 mg mL-1.Furthermore, the results are verified in the Erlenmeyer, working volume of 50 mL, pH = 8, T = 37 °C, agitation 150 rpm, t=18 hours, the activity of lipase and protein levels are 1.568 ± 0.014 U mL-1 and 0.072 ± 0.006 mg mL-1 respectively.The results showed that the optimum condition lipase activity was 1.568 U mL-1 so that the increase in the activity of only 75% compared to before optimization.
脂肪酶是一种广泛应用于化工、制药、食品、洗涤剂等行业的脂类水解酶。地衣芽孢杆菌F11.4是脂肪酶的潜在来源之一。本研究以鱼粉代替蛋白胨和酵母膏为基础，优化培养基组成和pH值，以获得地衣芽孢杆菌F11.4脂肪酶的最佳产量。选择显著因子采用2水平析因设计。采用中心复合设计(CCD)优化因子的上下限，采用响应面法(RSM)进行数据分析。发酵在erlenmeyer中进行，初始pH为8，温度为37°C，使用摇床培养箱，转速为150 rpm。当理想值接近1时，认为生产脂肪酶的发酵系统是最佳的。通过数值优化得到最佳培养基:OO:CPO含量为0.14% (w/v)，鱼粉含量为2% (w/v)， pH为8，转速为150 rpm，产酶活性为1.563 U mL-1，蛋白水平为0.08 mg mL-1的脂肪酶。在Erlenmeyer中验证，工作体积为50 mL, pH = 8，温度= 37℃，搅拌150 rpm，时间=18 h，脂肪酶活性和蛋白水平分别为1.568±0.014 U mL-1和0.072±0.006 mg mL-1。结果表明，最优条件下脂肪酶活性为1.568 U mL-1，较优化前仅提高75%。
{"title":"Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology","authors":"Trismilah Trismilah, P. Sarnianto, Edi Wahjono","doi":"10.5454/MI.10.4.4","DOIUrl":"https://doi.org/10.5454/MI.10.4.4","url":null,"abstract":"Lipase is a lipids hydrolyze enzyme which are widely used in various industries such as chemical, pharmaceutical, food industries, and detergents. Bacillus licheniformis F11.4 is one of the bacteria with potential source of lipase. This study aimed to obtain optimum production of lipase from B. licheniformis F11.4 by optimizing the composition of media and pH values with fish flour as a replacement for peptone and yeast extract based medium. Selection of the significant factors used a 2-level factorial design. The upper limit and lower limit of the selected factors was optimized using Central Composite Design (CCD) and the data analysis was performed using the Response Surface Methodology (RSM). Fermentation was carried out in erlenmeyer at initial pH 8 and a temperature of 37 °C, using a shaker incubator at 150 rpm. A fermentation system for lipases production is considered optimal when its desirability value closes to 1. By using numerical optimization, an optimal medium could be obtained, i.e. consisting of OO:CPO 0.14 % (w/v) and fish flour 2% (w/v), at pH 8 and 150 rpm, which produced lipase with enzyme activity of 1.563 U mL-1 and protein level of 0.08 mg mL-1.Furthermore, the results are verified in the Erlenmeyer, working volume of 50 mL, pH = 8, T = 37 °C, agitation 150 rpm, t=18 hours, the activity of lipase and protein levels are 1.568 ± 0.014 U mL-1 and 0.072 ± 0.006 mg mL-1 respectively.The results showed that the optimum condition lipase activity was 1.568 U mL-1 so that the increase in the activity of only 75% compared to before optimization.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"20 1","pages":"139-148"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83765740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cover/Table of Content and Editorial Board","authors":"I. Helianti","doi":"10.5454/MI.10.3.%P","DOIUrl":"https://doi.org/10.5454/MI.10.3.%P","url":null,"abstract":"","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90959034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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