Microbiological research最新文献_第3页

Endophytic fungus-induced phenazine-1-carboxylic acid production in Pseudomonas aeruginosa enhances biocontrol of rice spikelet rot disease 内生真菌诱导铜绿假单胞菌产非那嗪-1-羧酸增强水稻穗腐病的生物防治作用

IF 6.9 1区生物学 Q1 MICROBIOLOGY

Microbiological research

Pub Date : 2026-01-10 DOI: 10.1016/j.micres.2026.128439

Shi-Yi Huang , Zi-Han Zhao , Jia-Yan Xu , Yaseen Ullah , Yan-Jun Fei , Xiang-Yu Zhang , Xiao-Han Wu , Hui-Jun Jiang , Kai Sun , Xin-Yue Xu , Yong Zhang , Wei Zhang , Chuan-Chao Dai

Rice spikelet rot disease (RSRD) is a late-stage disease that affects rice spikes, leading to grain discoloration, deformation, and a reduced seed-setting rate. Recent studies suggest that the endophytic fungus Phomopsis liquidambaris plays a crucial role in reshaping the microbial community within rice spikes, promoting the enrichment of functionally active microorganisms with potent antagonistic properties, such as Pseudomonas aeruginosa. These beneficial microbes can effectively suppress pathogen infection, thus mitigating the impact of RSRD. Despite these findings, the interaction between Ph. liquidambaris and the key antagonistic microorganism P. aeruginosa remains unclear. In this study, we found that Ph. liquidambaris and P. aeruginosa acted synergistically to reduce the RSRD incidence, disease severity, and grain fumonisin content by 56.15, 44.44, and 23.65 %, respectively. To further investigate the underlying mechanism, we conducted coculture experiments, which revealed that Ph. liquidambaris stimulated P. aeruginosa to increase the production of phenazine-1-carboxylic acid (PCA), a key antimicrobial compound. PCA significantly inhibited the growth of Fusarium proliferatum and suppressed fumonisin biosynthesis. Pot experiments further confirmed its efficacy in preventing and controlling RSRD. Overall, this study demonstrated that fungalbacterial interactions stimulate the production of antimicrobial compounds, offering new insights into their potential for pathogen suppression and plant disease control.

水稻穗腐病（RSRD）是一种影响水稻穗的晚期疾病，导致籽粒变色、变形和结实率降低。最近的研究表明，内生真菌Phomopsis liquidambaris在重塑水稻穗内微生物群落，促进具有强拮抗特性的功能活性微生物（如铜绿假单胞菌）的富集方面起着至关重要的作用。这些有益微生物能够有效抑制病原菌感染，从而减轻RSRD的影响。尽管有这些发现，Ph. liquidambaris与关键拮抗微生物P. aeruginosa之间的相互作用仍不清楚。在本研究中，我们发现Ph. liquidambaris和P. aeruginosa协同作用，分别降低了56.15%、44.44%和23.65% %的RSRD发病率、疾病严重程度和谷物伏马菌素含量。为了进一步研究其潜在机制，我们进行了共培养实验，发现Ph. liquidambaris刺激P. aeruginosa增加了关键抗菌化合物phenazine-1-羧酸（PCA）的产量。PCA显著抑制了增生镰刀菌的生长，抑制了伏马菌素的合成。盆栽试验进一步证实了其防治RSRD的有效性。总的来说，这项研究表明，真菌与细菌的相互作用刺激了抗菌化合物的产生，为它们在抑制病原体和植物疾病控制方面的潜力提供了新的见解。

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引用次数: 0

Gut microbiota communities and their multifaceted roles in immune defense and social behavior of the red imported fire ant (Solenopsis invicta) 红火蚁肠道微生物群落及其在免疫防御和社会行为中的多重作用

IF 6.9 1区生物学 Q1 MICROBIOLOGY

Microbiological research

Pub Date : 2026-01-08 DOI: 10.1016/j.micres.2026.128438

Ezzeldin Ibrahim , Raghda Nasser , Jiayi Zhang , Solabomi Olaitan Ayoade , Lei Chen , Hui Yu , Liangliang Sang , Rahila Hafeez , Jinyan Luo , Jianfei Lu , Bin Li

The red imported fire ant (Solenopsis invicta) is an invasive species recognized for its aggressive behavior, posing significant risks to human health and local ecosystems. Pathogenic microbes, including fungi, bacteria, and viruses, play a critical role in the population of this pest and limiting its damage. Recent studies highlight the essential functions of gut microbiota, particularly bacteria, in enhancing S. invicta's immune responses to these pathogens. This review examines the diversity of gut microbiota in S. invicta, elucidating their contributions to immunity against pathogenic infections and their impact on the ant's overall activity and behavior. Previous studies indicate that the gut microbiota of S. invicta comprises various bacterial genera, including Pseudomonas aeruginosa, Actinobacteria, Staphylococcus, Lactococcus, Ralstonia, Achromobacter, and Lansdales, which play vital roles, particularly in digestion, nutrient synthesis, and immune function against pathogens. Furthermore, the composition of gut microbiota significantly influences foraging efficiency and social interactions within S. invicta colonies. Therefore, understanding the role of gut microbiota in the behavior and activity of S. invicta provides valuable insights for developing effective management strategies to control their populations. This review summarizes previous research on gut microbiota in S. invicta, focusing on its ecological significance and implications for future pest management studies.

红火蚁（Solenopsis invicta）是一种入侵物种，因其攻击行为而被公认，对人类健康和当地生态系统构成重大风险。病原微生物，包括真菌、细菌和病毒，在这种害虫的数量和限制其损害方面起着关键作用。最近的研究强调了肠道微生物群的基本功能，特别是细菌，在增强不可侵犯链球菌对这些病原体的免疫反应方面。本文综述了invicta蚂蚁肠道微生物群的多样性，阐明了它们对致病性感染免疫的贡献及其对蚂蚁整体活动和行为的影响。以往的研究表明，invicta的肠道微生物群包括铜绿假单胞菌、放线菌、葡萄球菌、乳球菌、Ralstonia、Achromobacter和Lansdales等多种细菌属，它们在消化、营养合成和对病原体的免疫功能方面起着至关重要的作用。此外，肠道菌群的组成显著影响不可侵犯葡萄菌落的觅食效率和社会互动。因此，了解肠道微生物群在不可侵犯链球菌的行为和活动中的作用，为制定有效的管理策略来控制其种群提供了有价值的见解。本文综述了invicta肠道菌群的研究进展，重点介绍了其生态学意义和对未来害虫防治研究的启示。

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引用次数: 0

Exploring the genomic features and plant growth-promoting properties of Enterobacter vonholyi Y16 isolated from maize rhizosphere 玉米根际冯霍利肠杆菌Y16的基因组特征及促植物生长特性研究

IF 6.9 1区生物学 Q1 MICROBIOLOGY

Microbiological research

Pub Date : 2026-01-04 DOI: 10.1016/j.micres.2026.128437

Jiacan Xu , Junnan Fang , Ruigang Wu , Yichen Wang , Chun Zhang , Xuming Wang , Tianlei Qiu

Maize yields rely heavily on chemical fertilizers, yet over half of the applied nitrogen remains unutilized, and excessive use harms soil, highlighting the need for sustainable alternatives. Plant growth-promoting rhizobacteria (PGPR) enhance plant growth and stress resistance, providing sustainable agricultural solutions. Here, we isolated Enterobacter vonholyi Y16 from the maize rhizosphere, and demonstrated multiple plant growth-promoting traits, including phosphate solubilization, potassium solubilization, siderophore secretion, and indole-3-acetic acid biosynthesis. An integrated approach combining rhizosphere microbiome profiling with plant transcriptomics was employed to elucidate the mechanisms of gene expression changes underlying PGPR-induced maize growth promotion. Inoculation with Y16 significantly increased primary root length in Arabidopsis thaliana by 16.7 %, and enhanced maize plant height, stem diameter, fresh shoot weight, and fresh root weight by 18.41 %, 22.32 %, 48.41 %, and 62.31 %, respectively. Strain Y16 successfully colonized the rhizosphere and influenced bacterial community composition under sterile soil conditions. Transcriptomic analysis revealed Y16-mediated regulation of key pathways, including plant hormone signaling, mitogen-activated protein kinase signaling, phenylpropanoid biosynthesis, and starch and sucrose metabolism. Notably, auxin-responsive genes were upregulated, correlating with Y16 abundance. These findings provide theoretical evidence for the molecular mechanisms of plant growth promotion by PGPR and offer insights for advancing sustainable agricultural development.

玉米产量严重依赖化肥，但施用的氮肥有一半以上未得到利用，过度使用对土壤有害，因此需要可持续的替代方案。促进植物生长的根瘤菌（PGPR）可以提高植物的生长和抗逆性，为可持续农业提供解决方案。在这里，我们从玉米根际分离出冯霍利肠杆菌Y16，并显示出多种促进植物生长的特性，包括磷酸盐增溶、钾增溶、铁载体分泌和吲哚-3-乙酸生物合成。采用根际微生物组分析和植物转录组学相结合的综合方法，阐明了pgpr诱导玉米生长促进的基因表达变化机制。接种Y16后，拟南芥初生根长增加了16.7 %，玉米株高、茎粗、鲜梢重和鲜根重分别增加了18.41 %、22.32 %、48.41 %和62.31 %。菌株Y16在无菌土壤条件下成功定植根际并影响细菌群落组成。转录组学分析揭示了y16介导的关键通路调控，包括植物激素信号、丝裂原激活的蛋白激酶信号、苯丙素生物合成以及淀粉和蔗糖代谢。值得注意的是，生长素响应基因上调，与Y16丰度相关。这些发现为探究PGPR促进植物生长的分子机制提供了理论依据，为促进农业可持续发展提供了参考。

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引用次数: 0

Akkermansia muciniphila: A double-edged sword in life-stage-specific nutritional modulation of Parkinson’s disease via the gut-brain axis 嗜muciniphila：通过肠-脑轴对帕金森病进行生命阶段特异性营养调节的双刃剑。

IF 6.9 1区生物学 Q1 MICROBIOLOGY

Microbiological research

Pub Date : 2025-12-29 DOI: 10.1016/j.micres.2025.128436

Nan Chen , Dejiang Pang , Huifang Shang

The gut mucin specialist Akkermansia muciniphila (A. muciniphila) exhibits a paradoxical duality in PD, showing both positive and negative correlations with motor and non-motor symptoms across distinct PD subtypes. This enigmatic role is further complicated by its dynamic lifespan trajectory: colonizing early life, peaking in adulthood, declining with aging, yet resurging in longevity cohorts. This review synthesizes evidence on A. muciniphila's structural components, its divergent associations with PD phenotypes, and the dietary and host factors shaping its abundance from gestation to senescence. We propose a lifespan-targeted intervention model that strategically modulates A. muciniphila, which could concurrently mitigate PD progression and promote healthy aging. We suggest suppressing its neurotoxic pathways in susceptible individuals while enhancing its beneficial functions in the aging process. Reconciling this microbial Janus face may pave the way for novel microbiome-based precision therapeutics against neurodegeneration.

肠道粘蛋白专家嗜粘杆菌（a.m uiniphila）在PD中表现出矛盾的二元性，在不同的PD亚型中显示出与运动和非运动症状的正相关和负相关。这种神秘的作用因其动态的生命轨迹而变得更加复杂：在生命早期占据主导地位，在成年期达到顶峰，随着年龄的增长而下降，但在长寿人群中又重新出现。本文综述了嗜粘液芽孢杆菌的结构成分，其与PD表型的不同关联，以及饮食和宿主因素对其从妊娠到衰老丰度的影响。我们提出了一种以寿命为目标的干预模型，该模型可以战略性地调节嗜粘液芽胞杆菌，从而同时缓解PD的进展并促进健康衰老。我们建议抑制易感个体的神经毒性通路，同时增强其在衰老过程中的有益功能。调和这种微生物双面面可能为新的基于微生物组的精确治疗神经变性铺平道路。

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引用次数: 0

Rhizosphere metagenomics and metabolomes provide new insights into the relationship between rhizosphere microecology and early bolting of Angelica dahurica 根际宏基因组学和代谢组学为白芷早期抽苔与根际微生态的关系提供了新的认识。

IF 6.9 1区生物学 Q1 MICROBIOLOGY

Microbiological research

Pub Date : 2025-12-24 DOI: 10.1016/j.micres.2025.128435

Weimeng Feng , Rui Ma , Yichen Guo , Bao Zhang , Jinxu Lan , Jun Liu , Suiqing Chen

Angelica dahurica is a medicinal and edible plant with a wide range of pharmaceutical and food applications. However, the early bolting, which leads to reduced yield and loss of bioactive constituents, has become a major obstacle to the industrial development of A. dahurica. Rhizosphere microecology affects plant growth and secondary metabolite accumulation, but the association of rhizosphere microecology with the early bolting of A. dahurica is not fully understood. This study integrated metagenomic and metabolomic analyses to systematically characterize the differences in rhizosphere microecology of non-bolting and early bolting A. dahurica plants. Results revealed significant disparities in soil physicochemical properties, root exudate profiles, and microbial community composition between two groups, all of which exhibited correlations with the coumarin compounds content, the primary pharmacologically active constituents of A. dahurica. Integrated analysis suggested that root-derived acyl-homoserine lactone (AHL) quorum-sensing signals, as the primary chemical signals of the prevalent Gram-negative bacteria, may participate in regulating the microbial community structure and soil properties, thereby influencing the bolting and flowering process. This study proposes a potential complex regulatory network of "rhizosphere microbiome – quorum-sensing signals – soil nitrogen cycle – bolting and flowering" linking the rhizosphere microecology to early bolting in A. dahurica, thereby addressing a key knowledge gap in this area. The findings offer a scientific foundation and innovative strategy for the simultaneous prevention of early bolting and quality improvement in A. dahurica through soil microecological management, which is of significant importance for promoting the sustainable commercial development of the A. dahurica industry.

白芷是一种药用和食用植物，具有广泛的制药和食品应用。然而，早抽苔导致的产量降低和活性成分的流失，已成为制约白芷产业化发展的主要障碍。根际微生态影响植物生长和次生代谢物积累，但根际微生态与白桦早期抽苔的关系尚不完全清楚。本研究采用宏基因组学和代谢组学相结合的方法，系统表征了未抽苔和早抽苔白芷根际微生态的差异。结果表明，两组土壤理化性质、根系分泌物特征和微生物群落组成存在显著差异，这些差异与白芷主要药理活性成分香豆素含量有关。综合分析认为，根源性酰基同丝氨酸内酯（AHL）群体感应信号可能作为流行的革兰氏阴性菌的主要化学信号，参与调控微生物群落结构和土壤性质，从而影响抽苔和开花过程。本研究提出了一个潜在的“根际微生物群-群体感应信号-土壤氮循环-抽苔和开花”的复杂调控网络，将根际微生态与白芷早期抽苔联系起来，从而解决了这一领域的关键知识空白。研究结果为通过土壤微生态管理防治白芷早抽和提高白芷品质提供了科学依据和创新策略，对促进白芷产业的可持续商业发展具有重要意义。

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引用次数: 0

RNA granule-associated regulation of antiviral RNA interference by the RNA-binding E3 ubiquitin ligase in Fusarium graminearum 小麦镰刀菌RNA结合E3泛素连接酶对抗病毒RNA干扰的RNA颗粒相关调控。

IF 6.9 1区生物学 Q1 MICROBIOLOGY

Microbiological research

Pub Date : 2025-12-22 DOI: 10.1016/j.micres.2025.128432

Gudam Kwon , Jisuk Yu , Kook-Hyung Kim

RNA interference (RNAi) is a major antiviral defense in fungi, yet the regulatory mechanisms governing this pathway remain incompletely characterized. In this study, we identified GzC2H056 as a host factor required for the induction of FgDICER-2 and FgAGO-1 in Fusarium graminearum during infection by the Fusarium graminearum virus 2 (FgV2). GzC2H056 expression is transcriptionally induced upon FgV2 infection and exhibits both RNA-binding and E3 ubiquitin ligase activity in vitro. Fluorescence tagging showed that GzC2H056 co-localizes with poly(A)-binding protein 1 and 5′-to-3′ exoribonuclease 1, which are markers of stress granules and P-bodies, respectively. RNA-sequencing analysis further revealed that GzC2H056 regulates the expression of genes involved in RNA metabolism and transcription factors linked to RNAi induction. Together, these findings identify GzC2H056 as a regulator of antiviral RNAi in fungi, suggesting that RNA granule-associated mechanisms contribute to fungal defense against mycoviruses.

RNA干扰（RNAi）是真菌的一种主要抗病毒防御手段，但控制这一途径的调控机制尚未完全确定。在这项研究中，我们确定了GzC2H056是在感染谷物镰刀菌病毒2 （FgV2）时诱导FgDICER-2和FgAGO-1所需的宿主因子。GzC2H056在FgV2感染后转录诱导表达，并在体外表现出rna结合和E3泛素连接酶活性。荧光标记显示GzC2H056与多聚(A)结合蛋白1和5′-3′外核糖核酸酶1共定位，这两个蛋白分别是胁迫颗粒和p体的标记物。RNA测序分析进一步揭示GzC2H056调控RNA代谢相关基因和RNAi诱导相关转录因子的表达。总之，这些发现确定GzC2H056是真菌抗病毒RNAi的调节因子，表明RNA颗粒相关机制有助于真菌防御分枝病毒。

引用次数: 0

Flufenamic acid fosters bacterial clearance by inhibiting Staphylococcus aureus AgrA and NLRP3 inflammasome of phagocytes 氟芬那酸通过抑制金黄色葡萄球菌AgrA和NLRP3吞噬细胞炎性体促进细菌清除。

IF 6.9 1区生物学 Q1 MICROBIOLOGY

Microbiological research

Pub Date : 2025-12-22 DOI: 10.1016/j.micres.2025.128434

Peng Gao , Suying Hou , Peirong Wang , Yuanxin Wei , Pradeep Halebeedu Prakash , Ivan Pok Man Lai , Bingpeng Yan , Sherlock Shing Chiu Tai , Victor Yat Man Tang , Sen Ye , Bill Hin Cheung Yam , Baozhong Zhang , King Chun Fung , Kong Hung Sze , Dan Yang , Jiandong Huang , Richard Yi Tsun Kao

The emergence of multi-resistant bacterial infections, particularly methicillin-resistant Staphylococcus aureus (MRSA), poses a significant global health crisis. Antibiotic treatments are increasingly ineffective, particularly against intracellular bacteria. This study unveils the therapeutic potential of flufenamic acid (FFA) against MRSA infections and its underlying mechanism of bacterial clearance in phagocytes. Our findings demonstrate that FFA suppresses toxin production by targeting the Agr pathway in an AgrA-dependent manner and triggers the production of reactive oxygen species (ROS) in immune cells through a mitochondrial cAMP-mediated mechanism. Additionally, FFA concurrently inhibits NLRP3, thereby enhancing bacterial clearance by immune cells. In vivo efficacy was evaluated using various staphylococcal infection models, demonstrating FFA's ability to potentiate bacterial clearance. Moreover, FFA synergizes with gentamicin both in vitro and in vivo. These findings strongly support FFA as a promising candidate for novel therapeutic agents against MRSA infections.

多重耐药细菌感染，特别是耐甲氧西林金黄色葡萄球菌（MRSA）的出现，构成了重大的全球卫生危机。抗生素治疗越来越无效，尤其是对细胞内细菌。这项研究揭示了氟芬那酸（FFA）对MRSA感染的治疗潜力及其在吞噬细胞中细菌清除的潜在机制。我们的研究结果表明，FFA以Agr依赖的方式靶向Agr途径抑制毒素的产生，并通过线粒体camp介导的机制触发免疫细胞中活性氧（ROS）的产生。此外，FFA同时抑制NLRP3，从而增强免疫细胞对细菌的清除。使用各种葡萄球菌感染模型评估了体内疗效，证明了FFA增强细菌清除的能力。此外，FFA与庆大霉素在体内外均有协同作用。这些发现有力地支持了FFA作为抗MRSA感染的新型治疗药物的前景。

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引用次数: 0

Streptococcus vaginalis affects cellular dynamics of cervical cancer cells via oxidative stress-induced activation of endoplasmic reticulum unfolded protein response 阴道链球菌通过氧化应激诱导的内质网未折叠蛋白反应激活影响宫颈癌细胞动力学

IF 6.9 1区生物学 Q1 MICROBIOLOGY

Microbiological research

Pub Date : 2025-12-22 DOI: 10.1016/j.micres.2025.128433

Jake Adolf V. Montecillo , Heon Jong Yoo , Yoo-Young Lee , Chul Min Park , Angela Cho , Hyunsu Lee , Jong Mi Kim , Nan Young Lee , Sun-Hyun Park , Nora Jee-Young Park , Hyung Soo Han , Gun Oh Chong , Incheol Seo

The vaginal microbiome plays an important role in the development of cervical cancer, highlighting the potential influence of specific members on disease susceptibility, progression, and suppression. In this study, we characterized a recently identified species of vaginal viridans group streptococci, Streptococcus vaginalis. By examining its prevalence, genomic features, and interactions with model cervical cancer cells, we aim to deepen the understanding of its biological significance and broader implications for vaginal health. Microbiome profiling detected S. vaginalis in 27 % of a cohort of Korean women, and the second most abundant species of Streptococcus. Pan-genome analysis and comparative genomics of S. vaginalis strains revealed their reduced pathogenic potentials. In vitro bioassays using cervical cancer cell models (HeLa, SiHa, and CaSki) demonstrated significant effects of S. vaginalis, influencing morphology, proliferation, migration, colony formation, and the induction of apoptosis. Mechanistic investigation identified the involvement of the endoplasmic reticulum (ER) stress and the activation of the unfolded protein response (UPR). Hydrogen peroxide produced by S. vaginalis was found to induce oxidative stress, triggering the ER stress-mediated cellular stress responses in cervical cancer cells. Our study revealed the influence of S. vaginalis on the dynamics of cervical cancer cells via oxidative stress-induced activation of the ER UPR pathway. These mechanistic insights emphasize a potential avenue for therapeutic interventions aimed at modulating oxidative and ER stress responses in cervical cancer treatment strategies. Overall, our findings provide new perspectives into the biological significance of S. vaginalis, expanding our understanding of its potential role beyond simple commensalism.

阴道微生物组在宫颈癌的发展中起着重要作用，突出了特定成员对疾病易感性、进展和抑制的潜在影响。在这项研究中，我们描述了一种最近发现的阴道翠绿菌群链球菌，阴道链球菌。通过研究其患病率、基因组特征和与模型宫颈癌细胞的相互作用，我们旨在加深对其生物学意义和对阴道健康的更广泛影响的理解。微生物组分析在27% %的韩国女性队列中检测到阴道链球菌，以及第二丰富的链球菌。阴道链球菌的泛基因组分析和比较基因组学分析显示其致病潜力降低。使用宫颈癌细胞模型（HeLa、SiHa和CaSki）进行的体外生物测定显示，阴道链球菌对宫颈癌细胞的形态、增殖、迁移、集落形成和诱导凋亡有显著影响。机制研究确定了内质网（ER）应激和未折叠蛋白反应（UPR）的激活。阴道链球菌产生的过氧化氢可诱导氧化应激，触发内质网应激介导的宫颈癌细胞应激反应。我们的研究揭示了阴道球菌通过氧化应激诱导的ER UPR通路激活对宫颈癌细胞动力学的影响。这些机制的见解强调了治疗干预的潜在途径，旨在调节宫颈癌治疗策略中的氧化和内质网应激反应。总的来说，我们的发现为阴道球菌的生物学意义提供了新的视角，扩展了我们对其潜在作用的理解，而不仅仅是简单的共生作用。

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引用次数: 0

Microbial biofilms as drought shields: Bacillus velezensis D103 enhances maize tolerance via aquaporin regulation 作为干旱屏障的微生物生物膜：velezensis芽孢杆菌D103通过水通道蛋白调节增强玉米耐受性

IF 6.9 1区生物学 Q1 MICROBIOLOGY

Microbiological research

Pub Date : 2025-12-20 DOI: 10.1016/j.micres.2025.128430

Yating Zhang , Ning Zhang , Zhiyong Zhang , Xinyue Bi , Zhenqiang Feng , Yanling Guo , Fangfang Yu , Bo Zhang , Tong Bi , Faryal Babar Baloch , Uswa Shafiq , Jianjia Miao , Yunjiao Wang , Bingxue Li , Yingfeng An

Drought stress severely constrains crop productivity, and while plant growth-promoting rhizobacteria (PGPR) are known to enhance drought tolerance by modulating host aquaporins (AQPs), the specific role of bacterial biofilm formation in this regulatory process remains poorly understood. Here, we demonstrate that biofilm formation is a pivotal mechanism through which Bacillus velezensis D103 confers drought resilience to maize. Under drought stress, maize root exudates synergistically enhanced D103 biofilm formation, which was essential for robust root colonization and mediated a drought-adaptive restructuring of the rhizosphere microbiome. Crucially, we found that an intact bacterial biofilm systemically upregulated key plant AQPs (ZmPIP2;6 and ZmTIP1;1), thereby enhancing root water transport capacity. Using virus-induced gene silencing, we further clarified the molecular mechanism underlying this biofilm-aquaporin link, revealing that ZmPIP2;6 is indispensable for D103-conferred drought tolerance. Our findings refine the current understanding of PGPR-mediated drought tolerance, highlighting that biofilms coordinate host AQP expression, rhizosphere microbiome assembly, and soil water retention to enhance drought resilience. This work provides a mechanistic basis for developing effective microbial inoculants.

干旱胁迫严重限制了作物产量，虽然已知植物促生长根茎细菌（PGPR）通过调节宿主水通道蛋白（AQPs）来增强耐旱性，但细菌生物膜形成在这一调节过程中的具体作用尚不清楚。在这里，我们证明了生物膜的形成是玉米芽孢杆菌D103赋予玉米抗旱性的关键机制。干旱胁迫下，玉米根系分泌物协同促进了D103生物膜的形成，这对根系定植和介导根际微生物群的干旱适应性重组至关重要。至关重要的是，我们发现完整的细菌生物膜系统性地上调了植物的关键AQPs (ZmPIP2；6和ZmTIP1；1)，从而增强了根的水运输能力。利用病毒诱导的基因沉默，我们进一步阐明了这种生物膜-水通道蛋白联系的分子机制，揭示了ZmPIP2；6对于d103授予的耐旱性是不可缺少的。我们的发现完善了目前对pgpr介导的耐旱性的理解，强调了生物膜协调宿主AQP表达、根际微生物群组装和土壤保水以增强抗旱能力。这项工作为开发有效的微生物接种剂提供了机理基础。

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引用次数: 0

Bacterial auxin catabolism as a driver of plant growth promotion and rhizosphere colonization fitness 细菌生长素分解代谢作为植物生长促进和根际定殖适应性的驱动因素。

IF 6.9 1区生物学 Q1 MICROBIOLOGY

Microbiological research

Pub Date : 2025-12-20 DOI: 10.1016/j.micres.2025.128431

Amalia Roca , Juan Gorts , Miguel A. Matilla

Inter-kingdom communication between plants and their associated microbiota is central to plant development and environmental adaptation. Indole-3-acetic acid (IAA) is the primary auxin in plants and regulates plant growth and development, while also modulating bacterial physiology and behavior. The concentration at which IAA exerts its biological effects in plants is critical and maintaining auxin homeostasis is essential. Although IAA production by plant growth-promoting bacteria typically stimulates plant growth, excessive IAA levels can be detrimental to plant physiology. Here, we investigate the in planta functional role of bacterial IAA catabolism using Pseudomonas putida 1290, a model plant-associated bacterium that degrades IAA through the Iac aerobic pathway. By constructing a mutant strain defective in the iac gene cluster, we show that IAA catabolism is essential for reversing auxin-mediated growth inhibition in tomato and maize, both in vitro and in microcosms. In addition, bacterial IAA degradation also prevented the formation of IAA-induced tumor-like structures in maize roots. Moreover, competitive colonization assays revealed that IAA catabolism enhances bacterial fitness in the rhizosphere, particularly under high-auxin conditions. Our findings establish bacterial IAA catabolism as a mechanism of metabolic signal interference that maintains auxin homeostasis in planta and promotes successful rhizosphere colonization. This work highlights the significance of microbial auxin metabolism in shaping plant–microbe interactions and its potential for application in sustainable agriculture strategies.

植物及其相关微生物群之间的王国间交流对植物发育和环境适应至关重要。吲哚-3-乙酸（IAA）是植物的主要生长素，在调节植物生长发育的同时，也调节细菌的生理和行为。IAA在植物体内发挥生物学作用的浓度是至关重要的，维持生长素的稳态是必不可少的。虽然植物生长促进细菌产生的IAA通常会刺激植物生长，但过量的IAA水平可能对植物生理有害。在这里，我们利用恶臭假单胞菌1290（一种通过Iac有氧途径降解IAA的模式植物相关细菌）研究了细菌IAA分解代谢在植物中的功能作用。通过构建iac基因簇缺陷突变株，我们发现IAA分解代谢对于逆转生长素介导的番茄和玉米生长抑制至关重要，无论是在体外还是在微观环境中。此外，细菌IAA降解也阻止了玉米根中IAA诱导的肿瘤样结构的形成。此外，竞争性定植试验表明，IAA分解代谢提高了根际细菌的适应性，特别是在高生长素条件下。我们的研究结果表明，细菌IAA分解代谢是一种代谢信号干扰机制，可以维持植物体内生长素的稳态，促进植物根际定植的成功。这项工作强调了微生物生长素代谢在形成植物-微生物相互作用中的重要性及其在可持续农业战略中的应用潜力。

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