首页 > 最新文献

Microbiological research最新文献

英文 中文
Brassinosteroids mediate arbuscular mycorrhizal symbiosis through multiple potential pathways and partial identification in tomato 芸苔素类固醇通过多种潜在途径介导丛枝菌根共生,并在番茄中进行了部分鉴定
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-10-05 DOI: 10.1016/j.micres.2024.127924
Ying Ren , Brian Tobin , Shuyi Yang , Tingying Xu , Hui Chen , Ming Tang
Currently, little is known regarding the specific processes through which brassinosteroids (BR) affect arbuscular mycorrhizal (AM) symbiosis. Understanding this relationship is vital for advancing plant physiology and agricultural applications. In this study, we aimed to elucidate the regulatory mechanisms of BR in AM symbiosis. According to the log2 fold change-value and adjP-value, we integrated the common differentially expressed genes (DEGs) in maize (Zea mays L.) treated with BR and AM, Arabidopsis (Arabidopsis thaliana) mutants deficient in BR receptors, and tomato (Solanum lycopersicum) plants inoculated with AM fungi. In addition, we characterized the symbiotic performance of tomato plants with BR receptor defects and overexpression. The results indicated that the common differential genes induced by BR and AM were involved in metabolic processes, such as cell wall modification, cytoskeleton remodeling, auxin and ethylene signaling, photosynthesis, mineral nutrient transport, and stress defense. Specifically, these include the BR1 gene, which modifies the cell wall. However, the fungal colonization rate of BR receptor-deficient tomato plants was significantly reduced, and the total phosphorus concentration was increased. Conversely, the performance of the overexpressing tomato transformation plants demonstrated a significant contrast. Additionally, the mild rescue of mycorrhizal attenuation in mutants treated with exogenous BR suggests the possibility of direct feedback from BR synthesis to AM. Notably, the cell wall modification gene (SlBR1) and calcium spike gene (SlIPD3) were induced by both BR and AM, suggesting that BR may influence cell penetration during the early stages of AM colonization. Synthesis: Our results demonstrated that BR positively regulates AM symbiosis through multiple pathways. These findings pave the way for future research, including isolation of the individual contributions of each pathway to this complex process and exploration of possible agricultural applications.
目前,人们对铜绿素类固醇(BR)影响丛枝菌根(AM)共生的具体过程知之甚少。了解这种关系对于促进植物生理学和农业应用至关重要。在本研究中,我们旨在阐明 BR 在 AM 共生中的调控机制。根据对折变化值(log2 fold change-value)和P值(adjP-value),我们整合了经BR和AM处理的玉米(Zea mays L.)、缺乏BR受体的拟南芥(Arabidopsis thaliana)突变体以及接种了AM真菌的番茄(Solanum lycopersicum)植株中常见的差异表达基因(DEGs)。此外,我们还研究了BR受体缺陷和过表达的番茄植株的共生表现。结果表明,BR和AM诱导的共同差异基因参与了代谢过程,如细胞壁修饰、细胞骨架重塑、辅助素和乙烯信号转导、光合作用、矿质养分运输和胁迫防御。具体来说,其中包括改造细胞壁的 BR1 基因。然而,BR 受体缺陷番茄植株的真菌定植率显著降低,总磷浓度增加。相反,过表达番茄转化植株的表现却呈现出明显的反差。此外,用外源 BR 处理的突变体对菌根衰减的轻度挽救表明,可能存在从 BR 合成到 AM 的直接反馈。值得注意的是,细胞壁修饰基因(SlBR1)和钙穗基因(SlIPD3)同时被BR和AM诱导,这表明BR可能会在AM定殖的早期阶段影响细胞渗透。综述:我们的研究结果表明,BR 通过多种途径积极调控 AM 的共生。这些发现为今后的研究铺平了道路,包括分离出每种途径对这一复杂过程的贡献,以及探索可能的农业应用。
{"title":"Brassinosteroids mediate arbuscular mycorrhizal symbiosis through multiple potential pathways and partial identification in tomato","authors":"Ying Ren ,&nbsp;Brian Tobin ,&nbsp;Shuyi Yang ,&nbsp;Tingying Xu ,&nbsp;Hui Chen ,&nbsp;Ming Tang","doi":"10.1016/j.micres.2024.127924","DOIUrl":"10.1016/j.micres.2024.127924","url":null,"abstract":"<div><div>Currently, little is known regarding the specific processes through which brassinosteroids (BR) affect arbuscular mycorrhizal (AM) symbiosis. Understanding this relationship is vital for advancing plant physiology and agricultural applications. In this study, we aimed to elucidate the regulatory mechanisms of BR in AM symbiosis. According to the log2 fold change-value and adjP-value, we integrated the common differentially expressed genes (DEGs) in maize (<em>Zea mays</em> L.) treated with BR and AM, Arabidopsis (<em>Arabidopsis thaliana</em>) mutants deficient in BR receptors, and tomato (<em>Solanum lycopersicum</em>) plants inoculated with AM fungi. In addition, we characterized the symbiotic performance of tomato plants with BR receptor defects and overexpression. The results indicated that the common differential genes induced by BR and AM were involved in metabolic processes, such as cell wall modification, cytoskeleton remodeling, auxin and ethylene signaling, photosynthesis, mineral nutrient transport, and stress defense. Specifically, these include the <em>BR1</em> gene, which modifies the cell wall. However, the fungal colonization rate of BR receptor-deficient tomato plants was significantly reduced, and the total phosphorus concentration was increased. Conversely, the performance of the overexpressing tomato transformation plants demonstrated a significant contrast. Additionally, the mild rescue of mycorrhizal attenuation in mutants treated with exogenous BR suggests the possibility of direct feedback from BR synthesis to AM. Notably, the cell wall modification gene (<em>SlBR1</em>) and calcium spike gene (<em>SlIPD3</em>) were induced by both BR and AM, suggesting that BR may influence cell penetration during the early stages of AM colonization. Synthesis: Our results demonstrated that BR positively regulates AM symbiosis through multiple pathways. These findings pave the way for future research, including isolation of the individual contributions of each pathway to this complex process and exploration of possible agricultural applications.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127924"},"PeriodicalIF":6.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chemoreceptor MCP4580 of Vibrio splendidus mediates chemotaxis toward L-glutamic acid contributing to bacterial virulence 绚丽弧菌的化学感受器 MCP4580 介导了对 L-谷氨酸的趋化作用,从而增强了细菌的毒力。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-10-01 DOI: 10.1016/j.micres.2024.127917
Ya Li , Weibo Shi , Zihao Sun , Weiwei Zhang
Chemotaxis has an essential function in flagellar bacteria that allows them to sense and respond to specific environmental signals, enabling their survival and colonization. Vibrio splendidus is an important opportunistic pathogen that infects a wide range of hosts including fish, bivalve, and sea cucumber. Our study demonstrated that V. splendidus AJ01 exhibited chemotaxis toward L-glutamic acid (L-Glu), an abundant amino acid in the intestinal and respiratory tree tissues of the sea cucumber. Bacterial samples collected from two locations in soft agar swimming plates were subjected to RNA-sequencing (RNA-Seq) analysis to identify the methyl-accepting chemotaxis protein (MCP) respond to L-Glu. Among the 40 annotated chemoreceptors, MCP4580 was identified as the MCP that mediates L-Glu-response. Molecular docking and site-directed mutagenesis revealed that L-arginine at residue 81 (R81) and L-glutamine at residue 88 (Q88) in the ligand-binding domain (LBD) are crucial for L-Glu recognition. Bacterial two-hybrid assay (BTH) showed that MCP4580 forms dimers and interacts with the histidine kinase CheA via the coupling protein CheW1 and CheW2. Phosphorylation analysis showed that the binding of L-Glu to MCP4580 results in the inhibition of CheA phosphorylation mainly via CheW1. Notably, sea cucumbers stimulated with each mutant strain of chemotaxis protein exhibited reduced mortality, highlighting the importance of chemotaxis in V. splendidus virulence. The present study provides valuable insights into the molecular components and signal transduction involved in the chemotaxis of V. splendidus toward L-Glu, and highlights the importance of chemotaxis in its virulence.
趋化作用是鞭毛细菌的一项基本功能,它能使细菌感知特定的环境信号并做出反应,从而使细菌得以生存和定殖。白芨弧菌是一种重要的机会性病原体,可感染鱼类、双壳贝类和海参等多种宿主。我们的研究表明,白芨弧菌 AJ01 对 L-谷氨酸(L-Glu)具有趋化性,而 L-谷氨酸是海参肠道和呼吸树组织中含量丰富的氨基酸。对从软琼脂游泳板两个位置采集的细菌样本进行了RNA测序(RNA-Seq)分析,以确定对L-谷氨酸有反应的甲基接受趋化蛋白(MCP)。在 40 个已注释的趋化感受器中,MCP4580 被确定为介导 L-Glu 反应的 MCP。分子对接和定点突变显示,配体结合结构域(LBD)中残基81(R81)处的L-精氨酸和残基88(Q88)处的L-谷氨酰胺对L-Glu的识别至关重要。细菌双杂交试验(BTH)显示,MCP4580 形成二聚体,并通过偶联蛋白 CheW1 和 CheW2 与组氨酸激酶 CheA 相互作用。磷酸化分析表明,L-Glu 与 MCP4580 结合后,主要通过 CheW1 抑制 CheA 的磷酸化。值得注意的是,用每种趋化蛋白突变株刺激的海参死亡率都有所降低,这突出表明了趋化作用在白芨弧菌毒力中的重要性。本研究对白芨趋化L-Glu的分子成分和信号转导提供了有价值的见解,并强调了趋化在其毒力中的重要性。
{"title":"Chemoreceptor MCP4580 of Vibrio splendidus mediates chemotaxis toward L-glutamic acid contributing to bacterial virulence","authors":"Ya Li ,&nbsp;Weibo Shi ,&nbsp;Zihao Sun ,&nbsp;Weiwei Zhang","doi":"10.1016/j.micres.2024.127917","DOIUrl":"10.1016/j.micres.2024.127917","url":null,"abstract":"<div><div>Chemotaxis has an essential function in flagellar bacteria that allows them to sense and respond to specific environmental signals, enabling their survival and colonization. <em>Vibrio splendidus</em> is an important opportunistic pathogen that infects a wide range of hosts including fish, bivalve, and sea cucumber<em>.</em> Our study demonstrated that <em>V. splendidus</em> AJ01 exhibited chemotaxis toward L-glutamic acid (L-Glu), an abundant amino acid in the intestinal and respiratory tree tissues of the sea cucumber. Bacterial samples collected from two locations in soft agar swimming plates were subjected to RNA-sequencing (RNA-Seq) analysis to identify the methyl-accepting chemotaxis protein (MCP) respond to L-Glu. Among the 40 annotated chemoreceptors, MCP4580 was identified as the MCP that mediates L-Glu-response. Molecular docking and site-directed mutagenesis revealed that L-arginine at residue 81 (R81) and L-glutamine at residue 88 (Q88) in the ligand-binding domain (LBD) are crucial for L-Glu recognition. Bacterial two-hybrid assay (BTH) showed that MCP4580 forms dimers and interacts with the histidine kinase CheA via the coupling protein CheW1 and CheW2. Phosphorylation analysis showed that the binding of L-Glu to MCP4580 results in the inhibition of CheA phosphorylation mainly via CheW1. Notably, sea cucumbers stimulated with each mutant strain of chemotaxis protein exhibited reduced mortality, highlighting the importance of chemotaxis in <em>V. splendidus</em> virulence. The present study provides valuable insights into the molecular components and signal transduction involved in the chemotaxis of <em>V. splendidus</em> toward L-Glu, and highlights the importance of chemotaxis in its virulence.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127917"},"PeriodicalIF":6.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The global transcription factor Clp exerts positive regulatory effects in the walnut bacterial black spot pathogen, Xanthomonas arboricola pv. juglandis 全局转录因子 Clp 在核桃细菌性黑斑病病原体 Xanthomonas arboricola pv. juglandis 中发挥积极的调控作用。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-10-01 DOI: 10.1016/j.micres.2024.127921
Feng Li , Wenzhong Yang , Benzhong Fu , Yonghong Yu , Yahui Mao
Walnut blight caused by the bacterium Xanthomonas arboricola pv. juglandis (Xaj) is one of the most common diseases of walnut (Juglans spp.), resulting in serious yield decline and significant economic losses. Crp-like protein (Clp) is an important global regulatory transcription factor in bacteria. In this study, we sought to elucidate the role of Clp in the pathogenicity of Xaj strain DW3F3 and the associated regulatory mechanism. The results indicated that clp gene deficiency significantly reduced the pathogenicity of Xaj DW3F3 in walnut without affecting the growth of the bacterium. We found that Clp positively regulates biofilm formation, extracellular polysaccharide production, exoenzyme secretion, and motility of Xaj, which was consistent with the transcript levels of virulence factor-encoding genes. However, overexpression of clp does not enhance the expression of all virulence genes, it may inhibit the expression of a part of virulence factor-related genes. EMSA assay further showed that Clp specifically binds to the promoters of these genes and regulates their expression, and CD spectra test certified that the ligand of Clp was c-di-GMP. Our findings contribute to the in-depth understanding of the pathogenic mechanism of Xaj and highlight the potential of Clp as a drug target for the development of agents to prevent and control walnut diseases.
由核桃黄单胞菌(Xanthomonas arboricola pv. juglandis,Xaj)引起的核桃枯萎病是核桃(Juglans spp.)最常见的病害之一,会导致严重减产和重大经济损失。Crp 样蛋白(Clp)是细菌中一种重要的全球调控转录因子。本研究试图阐明 Clp 在 Xaj 菌株 DW3F3 致病性中的作用及相关调控机制。结果表明,Clp 基因缺失会显著降低 Xaj DW3F3 在核桃中的致病性,而不会影响细菌的生长。我们发现 Clp 对 Xaj 的生物膜形成、胞外多糖产生、外酵素分泌和运动有正向调控作用,这与毒力因子编码基因的转录水平一致。然而,过表达 clp 并不会增强所有毒力基因的表达,它可能会抑制部分毒力因子相关基因的表达。EMSA测定进一步表明,Clp能特异性地与这些基因的启动子结合并调控它们的表达,CD光谱检测证明Clp的配体是c-di-GMP。我们的研究结果有助于深入了解 Xaj 的致病机理,并凸显了 Clp 作为药物靶点开发预防和控制核桃疾病的潜力。
{"title":"The global transcription factor Clp exerts positive regulatory effects in the walnut bacterial black spot pathogen, Xanthomonas arboricola pv. juglandis","authors":"Feng Li ,&nbsp;Wenzhong Yang ,&nbsp;Benzhong Fu ,&nbsp;Yonghong Yu ,&nbsp;Yahui Mao","doi":"10.1016/j.micres.2024.127921","DOIUrl":"10.1016/j.micres.2024.127921","url":null,"abstract":"<div><div>Walnut blight caused by the bacterium <em>Xanthomonas arboricola</em> pv. <em>juglandis</em> (<em>Xaj</em>) is one of the most common diseases of walnut (<em>Juglans</em> spp.), resulting in serious yield decline and significant economic losses. Crp-like protein (Clp) is an important global regulatory transcription factor in bacteria. In this study, we sought to elucidate the role of Clp in the pathogenicity of <em>Xaj</em> strain DW3F3 and the associated regulatory mechanism. The results indicated that <em>clp</em> gene deficiency significantly reduced the pathogenicity of <em>Xaj</em> DW3F3 in walnut without affecting the growth of the bacterium. We found that Clp positively regulates biofilm formation, extracellular polysaccharide production, exoenzyme secretion, and motility of <em>Xaj</em>, which was consistent with the transcript levels of virulence factor-encoding genes. However, overexpression of <em>clp</em> does not enhance the expression of all virulence genes, it may inhibit the expression of a part of virulence factor-related genes. EMSA assay further showed that Clp specifically binds to the promoters of these genes and regulates their expression, and CD spectra test certified that the ligand of Clp was c-di-GMP. Our findings contribute to the in-depth understanding of the pathogenic mechanism of <em>Xaj</em> and highlight the potential of Clp as a drug target for the development of agents to prevent and control walnut diseases.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127921"},"PeriodicalIF":6.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sterol regulatory element-binding proteins mediate intrinsic fungicide tolerance and antagonism in the fungal biocontrol agent Clonostachys rosea IK726 甾醇调节元件结合蛋白介导真菌生物控制剂 Clonostachys rosea IK726 对杀真菌剂的内在耐受性和拮抗性。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-09-28 DOI: 10.1016/j.micres.2024.127922
Edoardo Piombo , Georgios Tzelepis , Alma Gustavsson Ruus, Vahideh Rafiei, Dan Funck Jensen, Magnus Karlsson, Mukesh Dubey
Sterol regulatory element-binding proteins (SREBPs) are transcription factors governing various biological processes in fungi, including virulence and fungicide tolerance, by regulating ergosterol biosynthesis and homeostasis. While studied in model fungal species, their role in fungal species used for biocontrol remains elusive. This study delves into the biological and regulatory function of SREBPs in the fungal biocontrol agent (BCA) Clonostachys rosea IK726, with a specific focus on fungicide tolerance and antagonism. Clonostachys rosea genome contains two SREBP coding genes (sre1 and sre2) with distinct characteristics. Deletion of sre1 resulted in mutant strains with pleiotropic phenotypes, including reduced C. rosea growth on medium supplemented with prothioconazole and boscalid fungicides, hypoxia mimicking agent CoCl2 and cell wall stressor SDS, and altered antagonistic abilities against Botrytis cinerea and Rhizoctonia solani. However, Δsre2 strains showed no significant effect. Consistent with the gene deletion results, overexpression of sre1 in Saccharomyces cerevisiae enhanced tolerance to prothioconazole. The functional differentiation between SRE1 and SRE2 was elucidated by the yeast-two-hybridization assay, which showed an interaction between SREBP cleavage-activating protein (SCAP) and SRE1 but not between SRE2 and SCAP. Transcriptome analysis of the Δsre1 strain unveiled SRE1-mediated expression regulation of genes involved in lipid metabolism, respiration, and xenobiotic tolerance. Notably, genes coding for antimicrobial compounds chitinases and polyketide synthases were downregulated, aligning with the altered antagonism phenotype. This study uncovers the role of SREBPs in fungal BCAs, providing insights for C. rosea IK726 application into integrated pest management strategies.
甾醇调节元件结合蛋白(SREBPs)是一种转录因子,通过调节麦角甾醇的生物合成和平衡,控制真菌的各种生物过程,包括毒力和杀真菌剂耐受性。虽然对模型真菌物种进行了研究,但它们在用于生物防治的真菌物种中的作用仍然难以捉摸。本研究深入研究了 SREBPs 在真菌生物控制剂(BCA)Clonostachys rosea IK726 中的生物学和调控功能,特别关注杀真菌剂的耐受性和拮抗性。Clonostachys rosea 基因组包含两个 SREBP 编码基因(sre1 和 sre2),它们具有不同的特征。缺失 sre1 会导致突变株具有多生物表型,包括在添加了丙硫菌唑和溴螨脲杀菌剂、缺氧模拟剂 CoCl2 和细胞壁应激剂 SDS 的培养基上生长能力降低,以及对灰霉病菌和根瘤菌的拮抗能力改变。然而,Δsre2 菌株没有明显的影响。与基因缺失结果一致的是,sre1 在酿酒酵母中的过表达增强了对丙硫菌唑的耐受性。酵母双杂交试验阐明了 SRE1 和 SRE2 之间的功能差异,结果表明 SREBP 裂解激活蛋白(SCAP)与 SRE1 之间存在相互作用,而 SRE2 与 SCAP 之间没有相互作用。对 Δsre1 菌株的转录组分析揭示了 SRE1 介导的脂质代谢、呼吸和异生物耐受基因的表达调控。值得注意的是,编码抗微生物化合物几丁质酶和多酮合成酶的基因被下调,这与拮抗表型的改变相一致。这项研究揭示了 SREBPs 在真菌 BCA 中的作用,为 C. rosea IK726 应用于害虫综合治理战略提供了启示。
{"title":"Sterol regulatory element-binding proteins mediate intrinsic fungicide tolerance and antagonism in the fungal biocontrol agent Clonostachys rosea IK726","authors":"Edoardo Piombo ,&nbsp;Georgios Tzelepis ,&nbsp;Alma Gustavsson Ruus,&nbsp;Vahideh Rafiei,&nbsp;Dan Funck Jensen,&nbsp;Magnus Karlsson,&nbsp;Mukesh Dubey","doi":"10.1016/j.micres.2024.127922","DOIUrl":"10.1016/j.micres.2024.127922","url":null,"abstract":"<div><div>Sterol regulatory element-binding proteins (SREBPs) are transcription factors governing various biological processes in fungi, including virulence and fungicide tolerance, by regulating ergosterol biosynthesis and homeostasis. While studied in model fungal species, their role in fungal species used for biocontrol remains elusive. This study delves into the biological and regulatory function of SREBPs in the fungal biocontrol agent (BCA) <em>Clonostachys rosea</em> IK726, with a specific focus on fungicide tolerance and antagonism<em>. Clonostachys rosea</em> genome contains two SREBP coding genes (<em>sre1</em> and <em>sre2</em>) with distinct characteristics. Deletion of <em>sre1</em> resulted in mutant strains with pleiotropic phenotypes, including reduced <em>C. rosea</em> growth on medium supplemented with prothioconazole and boscalid fungicides, hypoxia mimicking agent CoCl<sub>2</sub> and cell wall stressor SDS, and altered antagonistic abilities against <em>Botrytis cinerea</em> and <em>Rhizoctonia solani</em>. However, Δ<em>sre2</em> strains showed no significant effect. Consistent with the gene deletion results, overexpression of <em>sre1</em> in <em>Saccharomyces cerevisiae</em> enhanced tolerance to prothioconazole. The functional differentiation between SRE1 and SRE2 was elucidated by the yeast-two-hybridization assay, which showed an interaction between SREBP cleavage-activating protein (SCAP) and SRE1 but not between SRE2 and SCAP. Transcriptome analysis of the Δ<em>sre1</em> strain unveiled SRE1-mediated expression regulation of genes involved in lipid metabolism, respiration, and xenobiotic tolerance. Notably, genes coding for antimicrobial compounds chitinases and polyketide synthases were downregulated, aligning with the altered antagonism phenotype. This study uncovers the role of SREBPs in fungal BCAs, providing insights for <em>C. rosea</em> IK726 application into integrated pest management strategies.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127922"},"PeriodicalIF":6.1,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deletion of PMP3 increases ketoconazole resistance by affecting plasma membrane potential in Candida albicans PMP3 的缺失会影响白色念珠菌的质膜电位,从而增加酮康唑的抗药性。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-09-27 DOI: 10.1016/j.micres.2024.127918
Mengsen Zhu, Yanting Wang, Jiacheng Zhao, Zhishang Shi, Congcong Ma, Qilin Yu, Mingchun Li
Ketoconazole is a classical antifungal drug commonly used in the clinic. With the increased use of ketoconazole in recent years, an increasing number of drug-resistant strains have emerged during clinical treatment. It is well known that fungi acquire drug resistance in multiple ways, while the molecular mechanisms underlying ketoconazole resistance remain for comprehensive exploration. In this study, we found that the expression of the small plasma membrane protein-encoding gene PMP3 was significantly down-regulated in several clinically isolated ketoconazole-resistant strains, indicating the relationship between PMP3 expression and ketoconazole resistance. By knocking out the PMP3, we found that the absence of the Pmp3 resulted in a significant increase in resistance of Candida albicans to ketoconazole, which was also confirmed in a systemic infection model in mice. We further demonstrated that various physiological properties, such as cell membrane fluidity, plasma membrane potential, permeability and ergosterol distribution were altered in the pmp3Δ/Δ mutant, which is associated with the enhanced cellular resistance to ketoconazole. In addition, overexpression rather than deletion of PMP3 alters the hyphal development and biofilm formation capacity in C. albicans. This study reveals the contribution of Pmp3 to alteration of drug resistance in fungal pathogens, which may guide the development of novel antifungal strategies.
酮康唑是临床上常用的经典抗真菌药物。近年来,随着酮康唑使用量的增加,临床治疗中出现了越来越多的耐药菌株。众所周知,真菌通过多种途径获得耐药性,而酮康唑耐药性的分子机制仍有待全面探索。本研究发现,在几株临床分离的酮康唑耐药菌株中,小质膜蛋白编码基因PMP3的表达明显下调,表明PMP3的表达与酮康唑耐药之间存在一定的关系。通过敲除 PMP3,我们发现 Pmp3 的缺失会导致白色念珠菌对酮康唑的耐药性明显增加,这在小鼠全身感染模型中也得到了证实。我们进一步证实,在 pmp3Δ/Δ 突变体中,细胞膜流动性、质膜电位、通透性和麦角固醇分布等各种生理特性都发生了改变,这与细胞对酮康唑的耐药性增强有关。此外,过表达而非缺失 PMP3 会改变白僵菌的头状花序发育和生物膜形成能力。这项研究揭示了 Pmp3 对真菌病原体耐药性的改变所起的作用,可为新型抗真菌策略的开发提供指导。
{"title":"Deletion of PMP3 increases ketoconazole resistance by affecting plasma membrane potential in Candida albicans","authors":"Mengsen Zhu,&nbsp;Yanting Wang,&nbsp;Jiacheng Zhao,&nbsp;Zhishang Shi,&nbsp;Congcong Ma,&nbsp;Qilin Yu,&nbsp;Mingchun Li","doi":"10.1016/j.micres.2024.127918","DOIUrl":"10.1016/j.micres.2024.127918","url":null,"abstract":"<div><div>Ketoconazole is a classical antifungal drug commonly used in the clinic. With the increased use of ketoconazole in recent years, an increasing number of drug-resistant strains have emerged during clinical treatment. It is well known that fungi acquire drug resistance in multiple ways, while the molecular mechanisms underlying ketoconazole resistance remain for comprehensive exploration. In this study, we found that the expression of the small plasma membrane protein-encoding gene <em>PMP3</em> was significantly down-regulated in several clinically isolated ketoconazole-resistant strains, indicating the relationship between <em>PMP3</em> expression and ketoconazole resistance. By knocking out the <em>PMP3</em>, we found that the absence of the Pmp3 resulted in a significant increase in resistance of <em>Candida albicans</em> to ketoconazole, which was also confirmed in a systemic infection model in mice. We further demonstrated that various physiological properties, such as cell membrane fluidity, plasma membrane potential, permeability and ergosterol distribution were altered in the <em>pmp3</em>Δ/Δ mutant, which is associated with the enhanced cellular resistance to ketoconazole. In addition, overexpression rather than deletion of <em>PMP3</em> alters the hyphal development and biofilm formation capacity in <em>C. albicans</em>. This study reveals the contribution of Pmp3 to alteration of drug resistance in fungal pathogens, which may guide the development of novel antifungal strategies.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127918"},"PeriodicalIF":6.1,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition of calpain reduces oxidative stress and attenuates pyroptosis and ferroptosis in Clostridium perfringens Beta-1 toxin-induced macrophages 抑制钙蛋白酶可降低氧化应激,减轻产气荚膜梭菌 Beta-1 毒素诱导的巨噬细胞的热蛋白沉积和铁蛋白沉积。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-09-27 DOI: 10.1016/j.micres.2024.127916
Siyu Zhang, Dong Wang, Yawen Ding, Yong Li, Yujiong Wang, Jin Zeng
Clostridium perfringens Beta-1 toxin (CPB1) is a lethal toxin, which can lead to necrotic enteritis, but the pathological mechanism has not been elucidated. We investigated whether reactive oxygen species (ROS) participated in CPB1-induced pyroptosis and ferroptosis, and investigated the effects of calpain on CPB1-induced oxidative stress and inflammation. Scavenging ROS by N-Acetyl-L cysteine (NAC) led to the reduction of ROS, inhibited the death of macrophages, cytoplasmic swelling and membrane rupture, the expression of pyroptosis-related proteins and proinflammatory factor, while increased the expression of anti-inflammatory factors in cells treated with rCPB1. Adenosine triphosphate (ATP) synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1 (ATP5A1) was identified specifically interact with rCPB1. Silencing ATP5A1 inhibited accumulation of ATP and ROS, leaded to less cytoplasmic swelling and membrane rupture, attenuated pyroptosis and inflammation in rCPB1-treated cells. We also found that rCPB1 induces ferroptosis in macrophages, and the level of ferroptosis was similar with H2O2. Of note, H2O2 is a major ROS source, indicated that ROS production may play a major role in the regulation of ferroptosis in macrophages treated with rCPB1. This finding was further corroborated in rCPB1- induced human acute monocytic leukemia cells, which were treated with NAC. In addition, the inhibition of ferroptosis using liproxstatin-1 inhibited the shriveled mitochondrial morphology, increased the expression of glutathione peroxidase 4, nicotinamide adenine dinucleotide (phosphate) hydrogen: quinone oxidoreductase 1 and cysteine/glutamic acid reverse transport solute carrier family 7 members 11, decreased the expression of heme oxygenase 1, nuclear receptor coactivator 4 and transferrin receptor proteins, reduced malondialdehyde and lipid peroxidation levels, and increased intracellular L-glutathione levels in cells treated with rCPB1. Furthermore, calpain inhibitor PD151746 was used to investigate how pyroptosis and ferroptosis were involved simultaneously in rCPB1-treated macrophages. We showed that PD151746 inhibited ATP and ROS production, reversed the representative pyroptosis/ferroptosis indicators and subsequently reduced inflammation. The above findings indicate that rCPB1 might lead to macrophage pyroptosis and ferroptosis through the large and sustained increase in intracellular calpain and oxidative stress, further lead to inflammation.
产气荚膜梭菌β-1毒素(CPB1)是一种致命毒素,可导致坏死性肠炎,但其病理机制尚未阐明。我们研究了活性氧(ROS)是否参与了 CPB1 诱导的热变态反应和铁变态反应,并探讨了钙蛋白酶对 CPB1 诱导的氧化应激和炎症的影响。用N-乙酰-L-半胱氨酸(NAC)清除ROS可减少ROS,抑制巨噬细胞的死亡、细胞质肿胀和膜破裂,抑制热凋亡相关蛋白和促炎因子的表达,同时增加经rCPB1处理的细胞中抗炎因子的表达。三磷酸腺苷(ATP)合成酶、H+转运、线粒体 F1 复合体α亚基 1(ATP5A1)与 rCPB1 有特异性相互作用。沉默 ATP5A1 可抑制 ATP 和 ROS 的积累,减少细胞质肿胀和细胞膜破裂,减轻 rCPB1 处理细胞的脓毒症和炎症。我们还发现,rCPB1 能诱导巨噬细胞中的嗜铁细胞,其嗜铁水平与 H2O2 相似。值得注意的是,H2O2 是一种主要的 ROS 来源,这表明 ROS 的产生可能在调节经 rCPB1 处理的巨噬细胞的铁卟啉沉积过程中发挥了重要作用。这一发现在用 NAC 处理的 rCPB1 诱导的人急性单核细胞白血病细胞中得到了进一步证实。此外,使用脂氧司他丁-1 抑制铁变态反应可抑制线粒体形态的萎缩,增加谷胱甘肽过氧化物酶 4、烟酰胺腺嘌呤二核苷酸(磷酸)氢、醌氧化还原酶的表达:醌氧化还原酶 1 和半胱氨酸/谷氨酸反向转运溶质运载家族 7 成员 11 的表达,降低血红素加氧酶 1、核受体辅激活因子 4 和转铁蛋白受体蛋白的表达,降低丙二醛和脂质过氧化水平,并增加细胞内 L-谷胱甘肽的水平。此外,我们还使用钙蛋白酶抑制剂 PD151746 来研究 rCPB1 处理的巨噬细胞如何同时参与了热蛋白沉积和铁蛋白沉积。我们发现,PD151746 抑制了 ATP 和 ROS 的产生,逆转了具有代表性的热蛋白沉积/铁蛋白沉积指标,从而减轻了炎症。上述研究结果表明,rCPB1 可通过细胞内钙蛋白酶和氧化应激的大量持续增加导致巨噬细胞热噬和铁噬,并进一步引发炎症。
{"title":"Inhibition of calpain reduces oxidative stress and attenuates pyroptosis and ferroptosis in Clostridium perfringens Beta-1 toxin-induced macrophages","authors":"Siyu Zhang,&nbsp;Dong Wang,&nbsp;Yawen Ding,&nbsp;Yong Li,&nbsp;Yujiong Wang,&nbsp;Jin Zeng","doi":"10.1016/j.micres.2024.127916","DOIUrl":"10.1016/j.micres.2024.127916","url":null,"abstract":"<div><div><em>Clostridium perfringens</em> Beta-1 toxin (CPB1) is a lethal toxin, which can lead to necrotic enteritis, but the pathological mechanism has not been elucidated. We investigated whether reactive oxygen species (ROS) participated in CPB1-induced pyroptosis and ferroptosis, and investigated the effects of calpain on CPB1-induced oxidative stress and inflammation. Scavenging ROS by N-Acetyl-L cysteine (NAC) led to the reduction of ROS, inhibited the death of macrophages, cytoplasmic swelling and membrane rupture, the expression of pyroptosis-related proteins and proinflammatory factor, while increased the expression of anti-inflammatory factors in cells treated with rCPB1. Adenosine triphosphate (ATP) synthase, H<sup>+</sup> transporting, mitochondrial F1 complex, alpha subunit 1 (ATP5A1) was identified specifically interact with rCPB1. Silencing ATP5A1 inhibited accumulation of ATP and ROS, leaded to less cytoplasmic swelling and membrane rupture, attenuated pyroptosis and inflammation in rCPB1-treated cells. We also found that rCPB1 induces ferroptosis in macrophages, and the level of ferroptosis was similar with H<sub>2</sub>O<sub>2</sub>. Of note, H<sub>2</sub>O<sub>2</sub> is a major ROS source, indicated that ROS production may play a major role in the regulation of ferroptosis in macrophages treated with rCPB1. This finding was further corroborated in rCPB1- induced human acute monocytic leukemia cells, which were treated with NAC. In addition, the inhibition of ferroptosis using liproxstatin-1 inhibited the shriveled mitochondrial morphology, increased the expression of glutathione peroxidase 4, nicotinamide adenine dinucleotide (phosphate) hydrogen: quinone oxidoreductase 1 and cysteine/glutamic acid reverse transport solute carrier family 7 members 11, decreased the expression of heme oxygenase 1, nuclear receptor coactivator 4 and transferrin receptor proteins, reduced malondialdehyde and lipid peroxidation levels, and increased intracellular L-glutathione levels in cells treated with rCPB1. Furthermore, calpain inhibitor PD151746 was used to investigate how pyroptosis and ferroptosis were involved simultaneously in rCPB1-treated macrophages. We showed that PD151746 inhibited ATP and ROS production, reversed the representative pyroptosis/ferroptosis indicators and subsequently reduced inflammation. The above findings indicate that rCPB1 might lead to macrophage pyroptosis and ferroptosis through the large and sustained increase in intracellular calpain and oxidative stress, further lead to inflammation.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127916"},"PeriodicalIF":6.1,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The expanding antimicrobial diversity of the genus Pantoea 盘尾丝菌属抗菌多样性的不断扩大。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-09-26 DOI: 10.1016/j.micres.2024.127923
Ashlyn Kirk, Emma Davidson, John Stavrinides
With the rise of antimicrobial resistance, there is high demand for novel antimicrobials to combat multi-drug resistant pathogens. The bacterial genus Pantoea produces a diversity of antimicrobial natural products effective against a wide range of bacterial and fungal targets. These antimicrobials are synthesized by specialized biosynthetic gene clusters that have unique distributions across Pantoea as well as several other genera outside of the Erwiniaceae. Phylogenetic and genomic evidence shows that these clusters can mobilize within and between species and potentially between genera. Pantoea antimicrobials belong to unique structural classes with diverse mechanisms of action, but despite their potential in antagonizing a wide variety of plant, human, and animal pathogens, little is known about many of these metabolites and how they function. This review will explore the known antimicrobials produced by Pantoea: agglomerins, andrimid, D-alanylgriseoluteic acid, dapdiamide, herbicolins, pantocins, and the various Pantoea Natural Products (PNPs). It will include information on the structure of each compound, their genetic basis, biosynthesis, mechanism of action, spectrum of activity, and distribution, highlighting the significance of Pantoea antimicrobials as potential therapeutics and for applications in biocontrol.
随着抗菌素耐药性的增加,人们对新型抗菌素的需求量很大,以对抗具有多重耐药性的病原体。盘尾丝菌属可产生多种抗菌天然产物,对多种细菌和真菌靶标有效。这些抗菌素是由专门的生物合成基因簇合成的,这些基因簇在盘尾丝菌属以及埃文菌科以外的其他几个属中都有独特的分布。系统发育和基因组学证据表明,这些基因簇可以在种内和种间调动,也可能在属间调动。盘菌抗菌素属于独特的结构类别,具有不同的作用机制,但尽管它们具有拮抗多种植物、人类和动物病原体的潜力,人们对其中许多代谢物及其功能却知之甚少。本综述将探讨泛影菌产生的已知抗菌素:agglomerins、andrimid、D-丙氨酰草酸、dapdiamide、hermicolins、pantocins 和各种泛影菌天然产物(PNPs)。它将包括每种化合物的结构、遗传基础、生物合成、作用机制、活性范围和分布等信息,突出泛影菌抗菌剂作为潜在疗法和生物防治应用的重要性。
{"title":"The expanding antimicrobial diversity of the genus Pantoea","authors":"Ashlyn Kirk,&nbsp;Emma Davidson,&nbsp;John Stavrinides","doi":"10.1016/j.micres.2024.127923","DOIUrl":"10.1016/j.micres.2024.127923","url":null,"abstract":"<div><div>With the rise of antimicrobial resistance, there is high demand for novel antimicrobials to combat multi-drug resistant pathogens. The bacterial genus <em>Pantoea</em> produces a diversity of antimicrobial natural products effective against a wide range of bacterial and fungal targets. These antimicrobials are synthesized by specialized biosynthetic gene clusters that have unique distributions across <em>Pantoea</em> as well as several other genera outside of the <em>Erwiniaceae</em>. Phylogenetic and genomic evidence shows that these clusters can mobilize within and between species and potentially between genera. <em>Pantoea</em> antimicrobials belong to unique structural classes with diverse mechanisms of action, but despite their potential in antagonizing a wide variety of plant, human, and animal pathogens, little is known about many of these metabolites and how they function. This review will explore the known antimicrobials produced by <em>Pantoea</em>: agglomerins, andrimid, D-alanylgriseoluteic acid, dapdiamide, herbicolins, pantocins, and the various <em>Pantoea</em> Natural Products (PNPs). It will include information on the structure of each compound, their genetic basis, biosynthesis, mechanism of action, spectrum of activity, and distribution, highlighting the significance of <em>Pantoea</em> antimicrobials as potential therapeutics and for applications in biocontrol.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127923"},"PeriodicalIF":6.1,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of Rhodococcus erythropolis stress genes in planctonic culture supplemented with various hydrocabons 补充了各种水囊的平面培养液中红球菌应激基因的表达。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-09-26 DOI: 10.1016/j.micres.2024.127920
Ivan Sazykin, Alla Litsevich, Ludmila Khmelevtsova, Tatiana Azhogina, Maria Klimova, Shorena Karchava, Margarita Khammami, Elena Chernyshenko, Ekaterina Naumova, Marina Sazykina
Studying Rhodococcus erythropolis stress response is of significant scientific interest, since this microorganism is widely used for bioremediation of oil-contaminated sites and is essential for environmental biotechnology. In addition, much less data was published on molecular mechanisms of stress resistance and adaptation to effects of pollutants for Gram-positive oil degraders compared to Gram-negative ones. This study provided an assessment of changes in the transcription level of the soxR, sodA, sodC, oxyR, katE, katG, recA, dinB, sigF, sigH genes in the presence of decane, hexadecane, cyclohexane, benzene, naphthalene, anthracene and diesel fuel. Judging by the changes in the expression of target genes, hydrocarbons as the main carbon source caused oxidative stress in R. erythropolis cells, which resulted in DNA damage. It was documented by enhanced transcription of genes encoding antioxidant enzymes (superoxide dismutase and catalase), SOS response, DNA polymerase IV, and sigma factors of RNA polymerase SigH and SigF. At this, it was likely that in the presence of hydrocarbons, transcription of catalase genes (katE and katG) was coordinated primarily by the sigF regulator.
研究红球菌(Rhodococcus erythropolis)的应激反应具有重要的科学意义,因为这种微生物被广泛用于石油污染场地的生物修复,对环境生物技术至关重要。此外,与革兰氏阴性微生物相比,有关革兰氏阳性石油降解微生物抗应激和适应污染物影响的分子机制的数据要少得多。本研究评估了在癸烷、十六烷、环己烷、苯、萘、蒽和柴油的作用下,soxR、sodA、sodC、oxyR、katE、katG、recA、dinB、sigF、sigH 基因转录水平的变化。从目标基因的表达变化来看,碳氢化合物作为主要碳源会对红腹灰杆菌细胞造成氧化应激,导致 DNA 损伤。这表现在编码抗氧化酶(超氧化物歧化酶和过氧化氢酶)、SOS 反应、DNA 聚合酶 IV 和 RNA 聚合酶 SigH 和 SigF 的基因转录增强。因此,在碳氢化合物存在的情况下,过氧化氢酶基因(katE 和 katG)的转录可能主要由 sigF 调节因子协调。
{"title":"Expression of Rhodococcus erythropolis stress genes in planctonic culture supplemented with various hydrocabons","authors":"Ivan Sazykin,&nbsp;Alla Litsevich,&nbsp;Ludmila Khmelevtsova,&nbsp;Tatiana Azhogina,&nbsp;Maria Klimova,&nbsp;Shorena Karchava,&nbsp;Margarita Khammami,&nbsp;Elena Chernyshenko,&nbsp;Ekaterina Naumova,&nbsp;Marina Sazykina","doi":"10.1016/j.micres.2024.127920","DOIUrl":"10.1016/j.micres.2024.127920","url":null,"abstract":"<div><div>Studying <em>Rhodococcus erythropolis</em> stress response is of significant scientific interest, since this microorganism is widely used for bioremediation of oil-contaminated sites and is essential for environmental biotechnology. In addition, much less data was published on molecular mechanisms of stress resistance and adaptation to effects of pollutants for Gram-positive oil degraders compared to Gram-negative ones. This study provided an assessment of changes in the transcription level of the <em>soxR</em>, <em>sodA</em>, <em>sodC</em>, <em>oxyR</em>, <em>katE</em>, <em>katG</em>, <em>recA</em>, <em>dinB</em>, <em>sigF, sigH</em> genes in the presence of decane, hexadecane, cyclohexane, benzene, naphthalene, anthracene and diesel fuel. Judging by the changes in the expression of target genes, hydrocarbons as the main carbon source caused oxidative stress in <em>R. erythropolis</em> cells, which resulted in DNA damage. It was documented by enhanced transcription of genes encoding antioxidant enzymes (superoxide dismutase and catalase), SOS response, DNA polymerase IV, and sigma factors of RNA polymerase SigH and SigF. At this, it was likely that in the presence of hydrocarbons, transcription of catalase genes (<em>katE</em> and <em>katG</em>) was coordinated primarily by the <em>sigF</em> regulator.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127920"},"PeriodicalIF":6.1,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Revealing quorum-sensing networks in Pseudomonas aeruginosa infections through internal and external signals to prevent new resistance trends 通过内部和外部信号揭示铜绿假单胞菌感染中的法定人数感应网络,防止出现新的抗药性趋势。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-09-24 DOI: 10.1016/j.micres.2024.127915
Li Guo, Qiao Ruan, Dandan Ma, Jun Wen
In the context of growing antibiotic resistance in bacteria, the quorum-sensing (QS) system of Pseudomonas aeruginosa (P. aeruginosa) has become a target for new therapeutic strategies. QS is a crucial communication process and an essential pathogenic mechanism. This comprehensive review explores the critical role of QS in the pathogenesis of P. aeruginosa infections, including lung, burn, bloodstream, gastrointestinal, corneal, and urinary tract infections. In addition, this review delves into the complexity of the bacterial QS communication network and highlights the intricate mechanisms underlying these pathological processes. Notably, in addition to the four main QS systems, bacterial QS can interact with various external and internal signaling networks, such as host environments and nutrients in the external microbiome, as well as internal virulence regulation systems within bacteria. These elements can significantly influence the behavior and virulence of microbial communities. Therefore, this review reveals that inhibitors targeting singular QS pathways may inadvertently promote virulence in other pathways, leading to new trends in drug resistance. In response to evolving resistance challenges, this study proposes more cautious treatment strategies, including multitarget interventions and combination therapies, aimed at combating the escalating issue of resistance.
在细菌的抗生素耐药性不断增加的背景下,铜绿假单胞菌(P. aeruginosa)的法定量感应(QS)系统已成为新治疗策略的目标。QS 是一个关键的交流过程,也是一种重要的致病机制。本综述全面探讨了 QS 在铜绿假单胞菌感染(包括肺部、烧伤、血流、胃肠道、角膜和泌尿道感染)发病机制中的关键作用。此外,这篇综述还深入探讨了细菌 QS 通信网络的复杂性,并强调了这些病理过程背后错综复杂的机制。值得注意的是,除了四大 QS 系统外,细菌 QS 还能与各种外部和内部信号网络相互作用,如宿主环境和外部微生物群中的营养物质,以及细菌内部的毒力调节系统。这些因素会极大地影响微生物群落的行为和毒力。因此,本综述揭示了针对单一 QS 途径的抑制剂可能会无意中促进其他途径的毒力,从而导致新的耐药性趋势。为应对不断演变的耐药性挑战,本研究提出了更为谨慎的治疗策略,包括多靶点干预和联合疗法,旨在应对不断升级的耐药性问题。
{"title":"Revealing quorum-sensing networks in Pseudomonas aeruginosa infections through internal and external signals to prevent new resistance trends","authors":"Li Guo,&nbsp;Qiao Ruan,&nbsp;Dandan Ma,&nbsp;Jun Wen","doi":"10.1016/j.micres.2024.127915","DOIUrl":"10.1016/j.micres.2024.127915","url":null,"abstract":"<div><div>In the context of growing antibiotic resistance in bacteria, the quorum-sensing (QS) system of <em>Pseudomonas aeruginosa (P. aeruginosa)</em> has become a target for new therapeutic strategies. QS is a crucial communication process and an essential pathogenic mechanism. This comprehensive review explores the critical role of QS in the pathogenesis of <em>P. aeruginosa</em> infections, including lung, burn, bloodstream, gastrointestinal, corneal, and urinary tract infections. In addition, this review delves into the complexity of the bacterial QS communication network and highlights the intricate mechanisms underlying these pathological processes. Notably, in addition to the four main QS systems, bacterial QS can interact with various external and internal signaling networks, such as host environments and nutrients in the external microbiome, as well as internal virulence regulation systems within bacteria. These elements can significantly influence the behavior and virulence of microbial communities. Therefore, this review reveals that inhibitors targeting singular QS pathways may inadvertently promote virulence in other pathways, leading to new trends in drug resistance. In response to evolving resistance challenges, this study proposes more cautious treatment strategies, including multitarget interventions and combination therapies, aimed at combating the escalating issue of resistance.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127915"},"PeriodicalIF":6.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Are1-mediated nitrogen metabolism is associated with iron regulation in the mycoparasite Trichoderma atroviride Are1介导的氮代谢与霉菌Trichoderma atroviride的铁调节有关。
IF 6.1 1区 生物学 Q1 MICROBIOLOGY Pub Date : 2024-09-24 DOI: 10.1016/j.micres.2024.127907
Clara Baldin , Rossana Segreto , Hoda Bazafkan , Martina Schenk , Julia Millinger , Ulrike Schreiner , Daniel Flatschacher , Verena Speckbacher , Siebe Pierson , Mostafa Alilou , Lea Atanasova , Susanne Zeilinger
Trichoderma atroviride is a mycoparasitic fungus with antagonistic activity against fungal pathogens and is used as a pathogen control agent alternative to synthetic fungicides. Sensing nutrient availability in the environment and adjusting metabolism for optimal growth, development and reproduction is essential for adaptability and is relevant to its mycoparasitic activity. During mycoparasitism, secondary metabolites are produced to weaken the fungal prey and support the attack. Are1-like proteins act as major GATA-type transcription factors in the activation of genes subject to nitrogen catabolite repression. Since the quality and quantity of nitrogen has been proven particularly relevant in remodeling the biosynthesis of secondary metabolites in fungi, we decided to functionally characterize Are1, the ortholog of Aspergillus nidulans AreA, in T. atroviride. We show that the growth of the T. atrovirideare1 mutant is impaired in comparison to the wild type on several nitrogen sources. Deletion of are1 enhanced sensitivity to oxidative and cell-wall stressors and altered the mycoparasitic activity. We were able to identify for the first time a link between Are1 and iron homeostasis via a regulatory mechanism that does not appear to be strictly linked to the nitrogen source, but rather to an independent role of the transcription factor.
毛霉菌是一种霉菌寄生真菌,对真菌病原体具有拮抗活性,可作为病原体控制剂替代合成杀真菌剂。感知环境中的养分供应情况并调整新陈代谢以获得最佳生长、发育和繁殖对其适应性至关重要,这也与它的霉菌寄生活动有关。在真菌寄生过程中,会产生次生代谢物来削弱真菌猎物并支持攻击。类 Are1 蛋白作为主要的 GATA 型转录因子激活受氮代谢抑制的基因。由于氮的质量和数量已被证明与重塑真菌次生代谢产物的生物合成特别相关,我们决定对阿特罗维里德曲霉 AreA 的直向同源物 Are1 进行功能鉴定。我们发现,与野生型相比,T. atroviride ∆are1 突变体在几种氮源上的生长都受到了影响。are1的缺失增强了对氧化和细胞壁胁迫的敏感性,并改变了寄生菌的活性。我们首次发现了 Are1 与铁稳态之间的联系,这种联系是通过一种调控机制实现的,这种机制似乎与氮源没有严格的联系,而是与转录因子的独立作用有关。
{"title":"Are1-mediated nitrogen metabolism is associated with iron regulation in the mycoparasite Trichoderma atroviride","authors":"Clara Baldin ,&nbsp;Rossana Segreto ,&nbsp;Hoda Bazafkan ,&nbsp;Martina Schenk ,&nbsp;Julia Millinger ,&nbsp;Ulrike Schreiner ,&nbsp;Daniel Flatschacher ,&nbsp;Verena Speckbacher ,&nbsp;Siebe Pierson ,&nbsp;Mostafa Alilou ,&nbsp;Lea Atanasova ,&nbsp;Susanne Zeilinger","doi":"10.1016/j.micres.2024.127907","DOIUrl":"10.1016/j.micres.2024.127907","url":null,"abstract":"<div><div><em>Trichoderma atroviride</em> is a mycoparasitic fungus with antagonistic activity against fungal pathogens and is used as a pathogen control agent alternative to synthetic fungicides. Sensing nutrient availability in the environment and adjusting metabolism for optimal growth, development and reproduction is essential for adaptability and is relevant to its mycoparasitic activity. During mycoparasitism, secondary metabolites are produced to weaken the fungal prey and support the attack. Are1-like proteins act as major GATA-type transcription factors in the activation of genes subject to nitrogen catabolite repression. Since the quality and quantity of nitrogen has been proven particularly relevant in remodeling the biosynthesis of secondary metabolites in fungi, we decided to functionally characterize Are1, the ortholog of <em>Aspergillus nidulans</em> AreA, in <em>T. atroviride.</em> We show that the growth of the <em>T. atroviride</em> ∆<em>are1</em> mutant is impaired in comparison to the wild type on several nitrogen sources. Deletion of <em>are1</em> enhanced sensitivity to oxidative and cell-wall stressors and altered the mycoparasitic activity. We were able to identify for the first time a link between Are1 and iron homeostasis via a regulatory mechanism that does not appear to be strictly linked to the nitrogen source, but rather to an independent role of the transcription factor.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127907"},"PeriodicalIF":6.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Microbiological research
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1