Microbial pathogenesis最新文献

{"title":"In vitro and in vivo assessment of the competence of a novel lytic phage vB_EcoS_UTEC10 targeting multidrug resistant Escherichia coli with a robust biofilm eradication activity","authors":"Azza A.H. Rajab ,&nbsp;Eslam K. Fahmy ,&nbsp;Safya E. Esmaeel ,&nbsp;Nehal Yousef ,&nbsp;Momen Askoura","doi":"10.1016/j.micpath.2024.107058","DOIUrl":"10.1016/j.micpath.2024.107058","url":null,"abstract":"<div><div><em>Escherichia coli</em> (<em>E. coli</em>) is a leading cause of human infections worldwide and is considered a major cause of nosocomial infections, sepsis, meningitis and diarrhea. Lately, there has been an alarming increase in the incidence of antimicrobial resistance among clinical <em>E. coli</em> isolates. In the current study, a novel bacteriophage (phage) vB_EcoS_UTEC10 was isolated and characterized. The isolated phage showed high stability over wide temperature and pH ranges beside its promising bacteriolytic activity against multidrug resistant (MDR) <em>E. coli</em> isolates. In addition, vB_EcoS_UTEC10 showed a marked antibiofilm capability against mature <em>E. coli</em> biofilms. Genomic investigation revealed that vB_EcoS_UTEC10 has a double stranded DNA genome that consists of 44,772 bp comprising a total of 73 open reading frames (ORFs), out of which 35 ORFs were annotated as structural or functional proteins, and none were related to antimicrobial resistance or lysogeny. <em>In vivo</em> investigations revealed a promising bacteriolytic activity of vB_EcoS_UTEC10 against MDR <em>E. coli</em> which was further supported by a significant reduction in bacterial load in specimens collected from the phage-treated mice. Histopathology examination demonstrated minimal signs of inflammation and necrosis in the tissues of phage-treated mice compared to the degenerative tissue damage observed in untreated mice. In summary, the present findings suggest that vB_EcoS_UTEC10 has a remarkable ability to eradicate MDR <em>E. coli</em> infections and biofilms. These findings could be further invested for the development of targeted phage therapies that offer a viable alternative to traditional antibiotics against resistant <em>E. coli</em>.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107058"},"PeriodicalIF":3.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and serological diagnosis of the circulating Trypanosoma evansi in Egyptian livestock with risk factors assessment","authors":"Emad B. Ata ,&nbsp;Tamer H. Abdel-Aziz ,&nbsp;Hoda S.M. Abdel-Ghany ,&nbsp;Bassma S.M. Elsawy ,&nbsp;Hend H.A.M. Abdullah ,&nbsp;Dina Abouelsoued ,&nbsp;Heba M. Ashry ,&nbsp;Mohamed R. Hassan ,&nbsp;Rafaat M. Shapaan ,&nbsp;Soad M. Nasr ,&nbsp;Mona S. Mahmoud ,&nbsp;Kadria N. Abdel Megeed ,&nbsp;Sobhy Abdel-Shafy","doi":"10.1016/j.micpath.2024.107073","DOIUrl":"10.1016/j.micpath.2024.107073","url":null,"abstract":"<div><div>This study aimed to characterize and determine the prevalence and the associated risk factors of trypanosomiasis in different Egyptian livestock from various governorates using molecular and serological techniques, adding to an assessment of some biochemical alterations in serum samples. A total 1019 blood samples were collected from nine governorates in the period from January 2020 to September 2021 from cattle (<em>n = 752</em>), buffaloes (<em>n = 42</em>), sheep (<em>n = 65</em>), goats (<em>n = 60</em>), donkeys (<em>n = 54</em>), and horses (<em>n = 46</em>). Molecular investigation followed by sequencing confirmed the presence of only <em>Trypanosoma evansi</em> (<em>T. evansi)</em> infection with a prevalence rate of 3.73 % (38/1019). Serologically, <em>T. evansi</em> was detected by indirect ELISA in 11 samples out of 519 negative PCR ones, with an overall prevalence 2.1 %. Furthermore, statistical difference was found between investigated governorates in both molecular and seroprevalence. Surprisingly, the risk of <em>T. evansi</em> infection was significantly (<em>P</em> &lt; 0.05) higher in winter (9.83 %) than in autumn (1.4 %), while no infection was recorded in the spring and summer seasons. No significant difference regarding animal hosts, age, and sex as risk factors was recorded. Serum biochemical analysis showed a non-significant decrease of total proteins, albumin, A/G ratio, and HDL-cholesterol in cattle, equine, and goats with a non-significant increase in total globulins level. In sheep, a significant increase was determined in all tested parameters except a significant decrease in the level of albumin and A/G ratio. To the best of our knowledge, this is the first large-scale epidemiological study determining the endemicity of the neglected <em>T. evansi</em> infection in different animal species in multiple governorates of Egypt. It is recommended to use both molecular and serological techniques for efficient diagnosis of trypanosomiasis as a preliminary approach to control the disease.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107073"},"PeriodicalIF":3.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequencing (WGS) analysis of antimicrobial resistance (AMR) milk and dairy-derived pathogens from Anand, Gujarat, India","authors":"Subrota Hati ,&nbsp;Shirin Vahora ,&nbsp;Janki Panchal ,&nbsp;Sandip Patel ,&nbsp;Arun Patel ,&nbsp;Harshad Chauhan ,&nbsp;Kishan Sharma ,&nbsp;Pritesh Sabara ,&nbsp;Mehul Shrimali","doi":"10.1016/j.micpath.2024.107076","DOIUrl":"10.1016/j.micpath.2024.107076","url":null,"abstract":"<div><div>This study aimed to evaluate the antimicrobial resistance (AMR) patterns and genomic characteristics of Enterococcus faecalis, Enterococcus faecium, and Staphylococcus aureus strains isolated from dairy products, including buttermilk, curd, ice cream, and sweets, in the Anand region of Gujarat, India. A total of 205 isolates were analysed, with the highest contamination levels found in buttermilk and curd. The bacterial isolates were identified using biochemical tests and advanced Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disk diffusion method, following CLSI guidelines, focusing on common antibiotics used for treating dairy-related bacterial infections. Resistance profiles were analysed using WHONET software.s The findings revealed significant multidrug resistance (MDR), particularly among E. faecium and E. faecalis, with over 95 % resistance to key antibiotics, including linezolid, ciprofloxacin, cefpodoxime, and carbapenems. Many strains were classified as MDR, XDR, and PDR. Staphylococcus aureus also exhibited substantial resistance to penicillin and enrofloxacin. whole-genome sequencing (WGS) and phylogenetic analysis to identify AMR determinants and conduct nucleotide sequence alignment. The study identified several antibiotic resistance genes, including LiaF, which regulates the expression of LiaR and LiaS genes. WGS revealed that genes such as GdpD, MprF, and PgsA encode intrinsic resistance determinants, contributing to antibiotic resistance. Additional AMR mechanisms were identified, including ABC transporter efflux pumps and the regulation of resistance genes by LiaR and LiaS. Phylogenetic analysis indicates a close relationship between Enterococcus faecium 640 1352.18624 and Enterococcus durans FB129-CNAB-4 883162.3.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107076"},"PeriodicalIF":3.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prebiotics: Comprehensive analysis of sources, structural characteristics and mechanistic roles in disease regulation","authors":"Magdalin Sylvia Singarayar ,&nbsp;Ajithan Chandrasekaran ,&nbsp;Dhivyadharshini Balasundaram ,&nbsp;Veeramurugan Veerasamy ,&nbsp;Vivek Neethirajan ,&nbsp;Sivasudha Thilagar","doi":"10.1016/j.micpath.2024.107071","DOIUrl":"10.1016/j.micpath.2024.107071","url":null,"abstract":"<div><div>Prebiotics are nondigestible components that comprise short-chain carbohydrates, primarily oligosaccharides, which are converted into beneficial compounds by probiotics. Various plant substances with prebiotic properties provide substantial health benefits and are used to prevent different diseases and for medical and clinical applications. Consuming prebiotics gives impeccable benefits since it aids in gut microbial balance. Prebiotic research is primarily concerned with the influence of intestinal disorders. The proposed review will describe recent data on the sources, structures, implementation of prebiotics and potential mechanisms in preventing and treating various disorders, with an emphasis on the gut microbiome. Prebiotics have a distinctive impact on the gastro intestine by explicitly encouraging the growth of probiotic organisms like <em>Bifidobacteria</em> and <em>Lactobacilli</em>. This in turn augments the body's inherent ability to fend off harmful pathogens. Prebiotic carbohydrates may also provide other non-specific advantages due to their fermentation in the large intestine. Additional <em>in vivo</em> research is needed to fully comprehend the interactions between prebiotics and probiotics ingested by hosts to improve their nutritional and therapeutic benefits.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107071"},"PeriodicalIF":3.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence and spread of resistant and biofilm-forming Acinetobacter baumannii in critically ill COVID-19 patients","authors":"Rafael Thiago Pereira da Silva ,&nbsp;Igor Vasconcelos Rocha ,&nbsp;Tainara Fernandes Dantas ,&nbsp;Jaqueline dos Santos Silva ,&nbsp;Sérgio Dias da Costa Júnior ,&nbsp;Ana Carolina de Oliveira Luz ,&nbsp;Marcelo Moreno ,&nbsp;Tereza Cristina Leal-Balbino ,&nbsp;Ana Vitória Araújo Lima ,&nbsp;Evandro G.da Silva Jr. ,&nbsp;Henrique Douglas Melo Coutinho ,&nbsp;Maria Betânia Melo de Oliveira","doi":"10.1016/j.micpath.2024.107078","DOIUrl":"10.1016/j.micpath.2024.107078","url":null,"abstract":"<div><div>The COVID-19 pandemic has raised concerns beyond the viral infection itself. Bacterial co-infections, particularly those involving <em>Acinetobacter baumannii</em>, have become a significant worry in critically ill COVID-19 patients. <em>A. baumannii</em> is an opportunistic pathogen that can cause nosocomial infections, especially in patients with compromised immune systems. This study investigated 36 <em>A. baumannii</em> isolates obtained from COVID-19 patients during a concurrent outbreak. The isolates were collected over two years through routine medical requests sent to the Clinical Microbiology laboratory. Identification of the strains was confirmed through biochemical tests, the Phoenix BD® Automated Microbiology System, and MALDI-TOF Mass Spectrometry. The study assessed the antimicrobial sensitivity of the isolates, with a specific focus on resistance to the beta-lactam group as well as aminoglycosides. The presence of specific antibiotic resistance genes (<em>bla</em>_OXA-23, _-24, _-51 and _-58, <em>bla</em>_KPC, <em>bla</em>_SHV, <em>bla</em>_IMP, <em>bla</em>_VIM, <em>aac</em>(6′)-<em>Ib, ant</em>(3″)-<em>Ia</em>, and <em>aph</em>(3′)-<em>Ia</em>) was investigated using PCR and Sanger DNA sequencing. Biofilm-forming capabilities of the isolates were also evaluated. The findings revealed diverse resistance profiles, with a high prevalence of resistant strains, including resistance to carbapenems. Genetic analysis suggested potential clonal spread of certain strains within the hospital setting. Moreover, a significant proportion of the isolates demonstrated strong biofilm-forming abilities, which can enhance persistence and antibiotic resistance. In conclusion, this study highlights the need for vigilant monitoring and targeted interventions to address bacterial co-infections in COVID-19 patients. The diversity in resistance patterns, potential clonal spread, and robust biofilm-forming abilities among <em>A. baumannii</em> isolates underscore the importance of addressing this issue to better manage and treat critically ill COVID-19 patients.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107078"},"PeriodicalIF":3.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of the novel recombinant LSDV in east and Southeast Asia: Inducing skin and testicular damage in golden hamsters","authors":"Jingyu Wang ,&nbsp;Shaobing Wan ,&nbsp;Shizhe Liu ,&nbsp;Zhen Wang ,&nbsp;Xiaoqing Ding ,&nbsp;Qianwen Wu ,&nbsp;Xin Liu ,&nbsp;Zihan Chen ,&nbsp;Ling Chen ,&nbsp;Heng Wang ,&nbsp;Kun Jia ,&nbsp;Shoujun Li","doi":"10.1016/j.micpath.2024.107057","DOIUrl":"10.1016/j.micpath.2024.107057","url":null,"abstract":"<div><div>In June 2020, Lumpy Skin Disease Virus (LSDV) was first discovered in Guangdong, China. Whole-genome sequencing and evolutionary analysis indicate that this strain represents a novel recombinant vaccine strain. Our investigation has identified regions affected by LSD outbreaks prior to 2024, revealing widespread prevalence of LSDV across East Asia and Southeast Asia. Subsequent analysis of the physical and chemical characteristics of the strain revealed LSDV's resilience to high temperatures, varying pH levels, humidity, UV radiation, and light exposure. Among the tested disinfectants, potassium persulfate, glutaraldehyde, and benzalkonium bromide have demonstrated the most potent virucidal efficacy against LSDV while maintaining high safety profiles. Utilizing the Syrian hamster model, infection with this strain has resulted in the formation of skin nodules and testicular damage, providing valuable insights into the pathogenesis of LSDV. In conclusion, the comprehensive experimental data presented herein serve as a cornerstone for advancing biosafety protocols, facilitating effective prevention and control strategies, and fostering the development of small animal model for LSDV.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107057"},"PeriodicalIF":3.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perturbed saliva microbiome is gender-specific in patients with oral lichen planus","authors":"Shiping Lv ,&nbsp;Li Yan ,&nbsp;Yunmei Dong ,&nbsp;Fangzhi Lou ,&nbsp;Shihong Luo ,&nbsp;Jingyi Xu ,&nbsp;Ning Kang ,&nbsp;Zhuoyan Luo ,&nbsp;Yiyun Liu ,&nbsp;Juncai Pu ,&nbsp;Xin Jin","doi":"10.1016/j.micpath.2024.107066","DOIUrl":"10.1016/j.micpath.2024.107066","url":null,"abstract":"<div><h3>Objective</h3><div>To understand the gender characteristics of oral lichen planus (OLP) by identifying the gender-specific salivary microbiome and its potential biomarkers.</div></div><div><h3>Methods</h3><div>A gender-based study was undertaken, commencing with the collection of saliva samples, followed by 16S rRNA gene sequencing, to explore the differences in the composition of saliva microbiome in OLP patients (40 males and 56 females) and healthy controls (40 males and 56 females), respectively.</div></div><div><h3>Results</h3><div>Both male and female OLP patients had significant differences in saliva microbiome composition from healthy controls, especially in female patients. Notably, <em>Pseudomonas</em> was only enriched in female patients. <em>Rhodococcus</em> (AUC: 0.91) and <em>Pseudomonas</em> (AUC: 0.97) had great potential as diagnostic biomarkers in male and female patients, respectively. The KEGG results showed metabolic dysfunction was more pronounced in female patients and a high level of microbial metabolism in diverse environments, ABC transporters, Quorum sensing and Two-component system. <em>Capnocytophaga</em> was negatively correlated with the erosion area in male patients. <em>Neisseria</em> was negatively correlated with the erosion area and <em>Rothia</em> was positively correlated with the pain level in female patients.</div></div><div><h3>Conclusions</h3><div>Our study revealed gender-specific perturbation in salivary microbiome within OLP patients, suggesting that the male and female patients with OLP may have different pathogenesis.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107066"},"PeriodicalIF":3.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using reverse vaccinology techniques combined with B-cell epitope prediction to screen potential antigenic proteins of the bovine pathogen Clostridium perfringens type A","authors":"He Qin ,&nbsp;Jingjing Ren ,&nbsp;Dongdong Zeng ,&nbsp;Ruijie Jin ,&nbsp;Qiuyan Deng ,&nbsp;Lihe Su ,&nbsp;Zengyang Luo ,&nbsp;Jianjun Jiang ,&nbsp;Pengyan Wang","doi":"10.1016/j.micpath.2024.107049","DOIUrl":"10.1016/j.micpath.2024.107049","url":null,"abstract":"<div><div><em>Clostridium perfringens</em> type A frequently causes necrohaemorrhagic enteritis in cattle, a rapidly progressing disease with a high mortality rate, thus inflicting substantial economic losses in the cattle industry. Effective prevention and control of this disease rely on rapid detection and vaccination strategies, making the screening of antigenic proteins with diagnostic and vaccine potential particularly crucial. In this study, we conducted a pangenomic analysis of 15 bacterial strains, grounded in traditional reverse vaccinology and supplemented with B-cell linear and conformational epitope analysis tools. This approach led to the identification of 2304 core genes and 3606 accessory genes, among which 58 surface-exposed proteins, encoded by core genes, were identified Proteins lacking tertiary structure information were predicted via AlphaFold2, ultimately identifying four target proteins and 14 candidate proteins enriched with linear and conformational epitopes, including virulence proteins such as alpha-toxin, theta-toxin, and alpha-clostripain, and extracellular solute-binding proteins, rhodanese-like proteins, and the accessory gene-encoded lysozyme inhibitor LprI family protein. Our findings demonstrate that the combined use of multiple B-cell epitope analysis tools can help overcome the limitations of any single tool. The proteins selected in this study offer valuable references for rapid diagnostics and the development of genetically engineered vaccines.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107049"},"PeriodicalIF":3.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycoplasma pneumoniae regulates the expression of GP130 in lung epithelial cells through apoptosis and TLR4/ NF-κB pathway during infection","authors":"Zhikun Zhang ,&nbsp;Dawei Shi ,&nbsp;Haiwei Dou ,&nbsp;Ruijie Wan ,&nbsp;Qing Yuan ,&nbsp;Peng Tu ,&nbsp;Deli Xin","doi":"10.1016/j.micpath.2024.107072","DOIUrl":"10.1016/j.micpath.2024.107072","url":null,"abstract":"<div><div>In previous study, lower levels of serum GP130 were reported in children with MPP. GP130 is an important signal transducer, the down regulation of which may influence host immune responses. In this study, we aimed to analyze the regulatory mechanism of GP130 during MP infection. Firstly, the mRNA and protein levels of GP130 both decrease and then increase with increasing multiplicity of infection (MOI: 1 to 40) of MP. The lowest levels of GP130 were detected at MOI of 5. Then, heat treated MP but not trypsin treated MP or MP extracted proteins show regulatory effect to the expression of GP130. These indicate that the down regulation of GP130 is related to protein mediate adhesion process of MP. Gene expression analysis revealed that MP affected apoptosis and the TLR4 pathway in infected cells, and the mRNA level of IL-6 was correlated with that of GP130. Further, Z-VAD-FMK (pan-caspase inhibitor) can suppress the apoptosis induced by MP infection and restore GP130 at protein level. Further studies revealed that MP infection promoted TLR4 internalization but did not activate the NF-κB pathway. The levels of surface TLR4 showed correlation with the transcription of IL-6 and GP130. TAK242 (TLR4 inhibitor) and PS341 (proteasome inhibitor) can restore the decreased transcription of GP130, both of which were able to promote NF-κB pathway activation in MP-infected cells. These suggested that the regulation of TLR4/NF-κB pathway and induced apoptosis post MP infection are involved in the down-regulation of GP130 at transcription and protein levels, respectively.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107072"},"PeriodicalIF":3.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing of clinical isolates of Klebsiella michiganensi in China carrying blaIPM-4 and blaNDM-1","authors":"Haigang Li ,&nbsp;Weiwei Dong ,&nbsp;Yang Liu ,&nbsp;Jie Ma ,&nbsp;Xudong Liu","doi":"10.1016/j.micpath.2024.107070","DOIUrl":"10.1016/j.micpath.2024.107070","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Aim&lt;/h3&gt;&lt;div&gt;This study aimed to analyze the prevalence, phenotypes, and carriage of resistance genes of carbapenem-resistant &lt;em&gt;Klebsiella michiganensis&lt;/em&gt; strains isolated in our hospital.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Method&lt;/h3&gt;&lt;div&gt;ology: Four &lt;em&gt;K. michiganensis&lt;/em&gt; strains were collected from January 2015 to December 2023. Antimicrobial susceptibility was tested using 21 antibiotics with the BD Phoenix™ M50 System. Whole-genome sequencing of the four strains was performed on an Illumina NovaSeq 6000 platform. Species identification was performed using Kleborate software, sequence type (ST) was performed using MLST, and prediction of antibiotic resistance genes and virulence genes were performed using ABRicate. PlasmidFinder was used to search for plasmids. Whole genome data from 211 strains of carbapenem-resistant &lt;em&gt;K. michiganensis&lt;/em&gt; were downloaded from the NCBI database; together with the strains in this study, these data were used to construct an phylogenetic tree using genome single nucleotide polymorphisms (SNP).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Antimicrobial susceptibility showed that &lt;em&gt;K. michiganensis&lt;/em&gt; was highly resistant to β-lactams. For the three carbapenems, strain WF0046 was only resistant to ertapenem, while WF0047, WF0052, and WF0053 were resistant to all three carbapenems tested. The four strains of &lt;em&gt;K. michiganensis&lt;/em&gt; only showed complete sensitivity to polymyxin E and tigecycline. The four &lt;em&gt;K. michiganensis&lt;/em&gt; strains were found to have 43 resistance genes, and all carried carbapenem resistance genes; WF0046 carried the carbapenem resistance gene &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;IMP-4&lt;/sub&gt;, while the other three strains carried &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;NDM-1&lt;/sub&gt;. Among the other resistance genes, β-lactam resistance genes were predicted most (11 types), followed by eight types of aminoglycoside resistance genes. Of the strains characterized in this study, WF0046 belonged to ST158, WF0047 belonged to ST40, WF0052 belonged to ST533, and WF0053 belonged to ST13. These four strains, along with 211 carbapenem-resistant &lt;em&gt;K. michiganensis&lt;/em&gt; strains downloaded from NCBI, were divided into five clades; WF0052 belonged to clade A, WF0046 belonged to clade C, and WF0047 and WF0053 both belonged to clade D. The four strains had relatively distant genetic relationships; WF0046, WF0047, and WF0053 were closely related to strains of human origin from other regions of China, while WF0052 was genetically close with a strain of &lt;em&gt;K. michiganensis&lt;/em&gt; isolated in the United States.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;The strains of &lt;em&gt;K. michiganensis&lt;/em&gt; from our hospital were resistant to multiple antibiotics, and all strains carried carbapenem resistance genes along with multiple other antibiotic resistance genes. The phylogenetic results showed that these four strains had distant genetic relationships and different ST types, indicating that they came from different sources and the possibility of polyclonal tran","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107070"},"PeriodicalIF":3.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}