Pub Date : 2022-03-13DOI: 10.20884/1.jm.2022.17.1.5601
H. Alfarisi, S. Sa’diah, B. Juliandi, T. Wresdiyati
Acalypha hispida Burm.f. is commonly used as an ornamental plant known for pharmacological effects. The nanoscale extract increases bioavailability and bioactivity. This research aimed to produce and characterize nanopowder extract of A. hispida leaves. Powdered leaves were macerated in 96% ethanol, then was evaporated in the spry dryer. Nanopowder extract was produced using planetary ball milling at 5000 rpm in different milling times, namely 5 minutes (nanopowder A), 10 minutes (nanopowder B), and 40 minutes (nanopowder C). The nanopowder extracts were evaluated using a particle size analyzer, scanning electron microscope, and high-performance liquid chromatography. The average particle size of A. hispida crude extract was 1271 nm, and nanopowder A, B, and C respectively were 837.1 nm, 803.8, and 512.2 nm. The polydispersity index of A. hispida crude extract, nanopowder A, B, and C were 0.754, 0.696, 0.717, dan 0.612. The milling process for 40 minutes reduced the content of 5% gallic acid and 10.3% catechin. The SEM image of nanopowder C was smaller than crude extract. The best average particle size of nanopowder C (512.2 nm) and polydispersity index (0.612) were produced using PBM for 40 minutes at 5000 rpm.
{"title":"Preparation and characterization of nanopowder of Acalypha hispida Leaves Extract Using Planetary Ball Milling","authors":"H. Alfarisi, S. Sa’diah, B. Juliandi, T. Wresdiyati","doi":"10.20884/1.jm.2022.17.1.5601","DOIUrl":"https://doi.org/10.20884/1.jm.2022.17.1.5601","url":null,"abstract":"Acalypha hispida Burm.f. is commonly used as an ornamental plant known for pharmacological effects. The nanoscale extract increases bioavailability and bioactivity. This research aimed to produce and characterize nanopowder extract of A. hispida leaves. Powdered leaves were macerated in 96% ethanol, then was evaporated in the spry dryer. Nanopowder extract was produced using planetary ball milling at 5000 rpm in different milling times, namely 5 minutes (nanopowder A), 10 minutes (nanopowder B), and 40 minutes (nanopowder C). The nanopowder extracts were evaluated using a particle size analyzer, scanning electron microscope, and high-performance liquid chromatography. The average particle size of A. hispida crude extract was 1271 nm, and nanopowder A, B, and C respectively were 837.1 nm, 803.8, and 512.2 nm. The polydispersity index of A. hispida crude extract, nanopowder A, B, and C were 0.754, 0.696, 0.717, dan 0.612. The milling process for 40 minutes reduced the content of 5% gallic acid and 10.3% catechin. The SEM image of nanopowder C was smaller than crude extract. The best average particle size of nanopowder C (512.2 nm) and polydispersity index (0.612) were produced using PBM for 40 minutes at 5000 rpm.","PeriodicalId":18773,"journal":{"name":"Molekul","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44945837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.20884/1.jm.2022.17.1.5574
Y. Male, I. W. Sutapa, M. Maahury, Muhamad Jamal, Dominggus Male
Computational studies have been carried out on eugenol and safrole derivatives as active sunscreen materials. The calculations were using Density Functional Theory (DFT) with the B3LYP functional and basis set 6-31G (d). The calculated eugenol derivative i.e. 3,4-dimethoxy isobutyl cinnamate; 3,4-dimethoxy isoamyl cinnamate; 3,4-dimethoxy isohexyl cinnamate; 3,4 dimethoxy isoheptyl cinnamate; 3,4-dimethoxy isoctyl cinnamate. The calculated safrole i.e. 3,4-methylenedioxy isobutyl cinnamate; 3,4-methylenedioxy isoamyl cinnamate; 3,4-methylenedioxy isohexyl cinnamate; 3,4-methylenedioxy isoheptyl cinnamate; and 3,4-methylenedioxy isooctyl cinnamate. The main parameter in determining the potential of sunscreen compounds is energy gap. The results of computational calculations show that the 3,4-methylenedioxy isohexyl cinnamate has the smallest energy gap (HOMO-LUMO) of 0.15021 eV. This shows that 3,4-methylenedioxy isohexyl cinnamate has a better potential as an active sunscreen material. In addition, the elongation of the alkyl chain does not significantly affect the HOMO-LUMO energy difference
{"title":"Computational Study Potency of Eugenol and Safrole Derivatives as Active Sunscreen Material","authors":"Y. Male, I. W. Sutapa, M. Maahury, Muhamad Jamal, Dominggus Male","doi":"10.20884/1.jm.2022.17.1.5574","DOIUrl":"https://doi.org/10.20884/1.jm.2022.17.1.5574","url":null,"abstract":"Computational studies have been carried out on eugenol and safrole derivatives as active sunscreen materials. The calculations were using Density Functional Theory (DFT) with the B3LYP functional and basis set 6-31G (d). The calculated eugenol derivative i.e. 3,4-dimethoxy isobutyl cinnamate; 3,4-dimethoxy isoamyl cinnamate; 3,4-dimethoxy isohexyl cinnamate; 3,4 dimethoxy isoheptyl cinnamate; 3,4-dimethoxy isoctyl cinnamate. The calculated safrole i.e. 3,4-methylenedioxy isobutyl cinnamate; 3,4-methylenedioxy isoamyl cinnamate; 3,4-methylenedioxy isohexyl cinnamate; 3,4-methylenedioxy isoheptyl cinnamate; and 3,4-methylenedioxy isooctyl cinnamate. The main parameter in determining the potential of sunscreen compounds is energy gap. The results of computational calculations show that the 3,4-methylenedioxy isohexyl cinnamate has the smallest energy gap (HOMO-LUMO) of 0.15021 eV. This shows that 3,4-methylenedioxy isohexyl cinnamate has a better potential as an active sunscreen material. In addition, the elongation of the alkyl chain does not significantly affect the HOMO-LUMO energy difference","PeriodicalId":18773,"journal":{"name":"Molekul","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47084965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.20884/1.jm.2022.17.1.5604
J. Chanathaworn, C. Yatongchai, S. Samanman
Giant leucaena wood was utilized to prepare heterogeneous catalysts through a fast pyrolysis method and chemical activation for transesterification. The obtained catalysts were investigated using SEM, CHNS/O analyzer, XRF and XRD. The influence of the concentration of KOH (3-9 M), catalyst amount (0.25-2.0 g), methanol to oil ratio (4:1-10:1), and reaction time (30-75 min) on FAME yield was also studied on transesterification reaction carried out at 60ºC under a 750 rpm stirring speed. The experiment results demonstrate that chemical activation was required to improve the porosity of the catalyst. The result showed that a well-developed porous structure was observed, as the concentration of KOH increased activated biochar become more porous. 7M-KOH for chemical activation was the best condition to obtain a porous catalyst. It was found that the main factors affecting the FAME yield were dependent on various parameters including methanol: oil ratio, catalyst loading, reaction time and stirring speed via transesterification process. The highest yield of 94.06% was achieved on 0.5g of the catalyst activated by 7M-KOH, a methanol:oil ratio of 6:1 and a 1-hour reaction. The obtained biodiesel mainly composed of different fatty acid in follow order C18:1 > C16:0 > C18:2 > C18:0. Properties reached the ASTM D6751-12 and EN 14214:2012 standard, indicating that leucaena-derived biochar is potentially utilized in biodiesel production.
{"title":"Leucaena–Derived Biochar for Biodiesel Production","authors":"J. Chanathaworn, C. Yatongchai, S. Samanman","doi":"10.20884/1.jm.2022.17.1.5604","DOIUrl":"https://doi.org/10.20884/1.jm.2022.17.1.5604","url":null,"abstract":"Giant leucaena wood was utilized to prepare heterogeneous catalysts through a fast pyrolysis method and chemical activation for transesterification. The obtained catalysts were investigated using SEM, CHNS/O analyzer, XRF and XRD. The influence of the concentration of KOH (3-9 M), catalyst amount (0.25-2.0 g), methanol to oil ratio (4:1-10:1), and reaction time (30-75 min) on FAME yield was also studied on transesterification reaction carried out at 60ºC under a 750 rpm stirring speed. The experiment results demonstrate that chemical activation was required to improve the porosity of the catalyst. The result showed that a well-developed porous structure was observed, as the concentration of KOH increased activated biochar become more porous. 7M-KOH for chemical activation was the best condition to obtain a porous catalyst. It was found that the main factors affecting the FAME yield were dependent on various parameters including methanol: oil ratio, catalyst loading, reaction time and stirring speed via transesterification process. The highest yield of 94.06% was achieved on 0.5g of the catalyst activated by 7M-KOH, a methanol:oil ratio of 6:1 and a 1-hour reaction. The obtained biodiesel mainly composed of different fatty acid in follow order C18:1 > C16:0 > C18:2 > C18:0. Properties reached the ASTM D6751-12 and EN 14214:2012 standard, indicating that leucaena-derived biochar is potentially utilized in biodiesel production.","PeriodicalId":18773,"journal":{"name":"Molekul","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47434185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.20884/1.jm.2022.17.1.5605
S. Samanman, Nuttaporn Tepprom, Inun Yipong, Yasmee Saleah, Kholeeyah Navani, Nifanusi Samae, Amri Hj Mohammed, A. S. Abd Aziz
In Thailand, the rubber tree is widely used as a source of natural rubber and their seeds have been found to be rich in oil production. The seed shell is the residue and not edible but abundantly available in the country. Therefore, the objective of this work was to prepare activated carbon (AC) from rubber seed shells. ACs were produced by chemical activation and their characteristics were investigated. The effects of activation temperature and chemical impregnation time in the agent were examined. Proximate analysis was investigated and the results were within the standard of AC. The adsorption capacity was demonstrated with BET, iodine, and methylene blue number. The best produced AC (700 °C of activation temperature and 24 h of impregnation time) provided the highest BET surface area, iodine and methylene blue number were 923 m2/g, 1,234±24 mg/g and 1,204±10 mg/g, respectively. FT-IR spectrum indicates the presence of oxygen and nitrogen containing surface functional groups such as -OH, -CH3, -CH2, C=C, C-O, and N-H groups. The microstructure of the produced ACs was examined by SEM and the results found that they are porosity. The antibacterial effect results were discovered to be also effective against E. coli and S. aureus after 3 h and 24 h of contact times, respectively. In addition, the prepared AC also provided many properties that are better than those obtained by the commercial AC. Finally, it can be concluded that, AC from rubber seed shell can be applied as a low cost and possible alternate adsorbent.
{"title":"Rubber (Hevea brasiliensis) Seed shell Activated Carbon Preparation, Characterization and Antibacterial Activity Studies","authors":"S. Samanman, Nuttaporn Tepprom, Inun Yipong, Yasmee Saleah, Kholeeyah Navani, Nifanusi Samae, Amri Hj Mohammed, A. S. Abd Aziz","doi":"10.20884/1.jm.2022.17.1.5605","DOIUrl":"https://doi.org/10.20884/1.jm.2022.17.1.5605","url":null,"abstract":"In Thailand, the rubber tree is widely used as a source of natural rubber and their seeds have been found to be rich in oil production. The seed shell is the residue and not edible but abundantly available in the country. Therefore, the objective of this work was to prepare activated carbon (AC) from rubber seed shells. ACs were produced by chemical activation and their characteristics were investigated. The effects of activation temperature and chemical impregnation time in the agent were examined. Proximate analysis was investigated and the results were within the standard of AC. The adsorption capacity was demonstrated with BET, iodine, and methylene blue number. The best produced AC (700 °C of activation temperature and 24 h of impregnation time) provided the highest BET surface area, iodine and methylene blue number were 923 m2/g, 1,234±24 mg/g and 1,204±10 mg/g, respectively. FT-IR spectrum indicates the presence of oxygen and nitrogen containing surface functional groups such as -OH, -CH3, -CH2, C=C, C-O, and N-H groups. The microstructure of the produced ACs was examined by SEM and the results found that they are porosity. The antibacterial effect results were discovered to be also effective against E. coli and S. aureus after 3 h and 24 h of contact times, respectively. In addition, the prepared AC also provided many properties that are better than those obtained by the commercial AC. Finally, it can be concluded that, AC from rubber seed shell can be applied as a low cost and possible alternate adsorbent.","PeriodicalId":18773,"journal":{"name":"Molekul","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49203544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.20884/1.jm.2022.17.1.5597
Monica Avissa, M. Alauhdin
Contamination of the environment by hazardous metal ions has been a major environmental issue for the past several decades. Among several hazardous metals, mercury ion (Hg(II)) is of particular concern as its compounds are extremely toxic. Hence, developing detection methods for traces of Hg(II) ions in aquatic systems is critical for mercury pollution mitigation. One method that can be used to monitor Hg(II) in aquatic systems is colorimetry-based method which is simple, rapid, and low-cost, yet selective and sensitive. The method can be conducted by applying the Localized Surface Plasmon Resonance phenomenon of metal nanoparticles, such as silver nanoparticles. There are non-Hg(II) ions in the aquatic environment that can interfere the measurements. Thus, a selective method is needed to obtain a valid measurement result. Here, we introduced silver nanoparticles-chitosan (AgNPs-Ch) synthesized by chemical reduction as a selective probe of Hg(II) in an aqueous solution. The AgNPs-Ch was synthesized from silver nitrate at 80°C using trisodium citrate and chitosan as reducing agent and stabilizer, respectively. The synthesized nanoparticles were spherical with an average size below 15.0 nm. Moreover, the AgNPs-Ch was selective for Hg(II) with a linearity of 0.9556 in the concentration range of 1 - 5 ppm and was able to detect the ion down to 1.33 ppm.
{"title":"Selective Colorimetric Detection of Mercury(II) using Silver Nanoparticles-Chitosan","authors":"Monica Avissa, M. Alauhdin","doi":"10.20884/1.jm.2022.17.1.5597","DOIUrl":"https://doi.org/10.20884/1.jm.2022.17.1.5597","url":null,"abstract":"Contamination of the environment by hazardous metal ions has been a major environmental issue for the past several decades. Among several hazardous metals, mercury ion (Hg(II)) is of particular concern as its compounds are extremely toxic. Hence, developing detection methods for traces of Hg(II) ions in aquatic systems is critical for mercury pollution mitigation. One method that can be used to monitor Hg(II) in aquatic systems is colorimetry-based method which is simple, rapid, and low-cost, yet selective and sensitive. The method can be conducted by applying the Localized Surface Plasmon Resonance phenomenon of metal nanoparticles, such as silver nanoparticles. There are non-Hg(II) ions in the aquatic environment that can interfere the measurements. Thus, a selective method is needed to obtain a valid measurement result. Here, we introduced silver nanoparticles-chitosan (AgNPs-Ch) synthesized by chemical reduction as a selective probe of Hg(II) in an aqueous solution. The AgNPs-Ch was synthesized from silver nitrate at 80°C using trisodium citrate and chitosan as reducing agent and stabilizer, respectively. The synthesized nanoparticles were spherical with an average size below 15.0 nm. Moreover, the AgNPs-Ch was selective for Hg(II) with a linearity of 0.9556 in the concentration range of 1 - 5 ppm and was able to detect the ion down to 1.33 ppm.","PeriodicalId":18773,"journal":{"name":"Molekul","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46604948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.20884/1.jm.2022.17.1.5603
N. Happyana, Y. M. Syah, E. Hakim
Gayo (Aceh) coffee is one of the best coffees from Indonesia. In this work, metabolites in the Gayo roasted arabica and robusta coffees were identified with 1H NMR spectroscopy analysis. Accumulatively 28 compounds were successfully detected, including the major and minor metabolites of the roasted coffee. Multivariate data analysis was applied to evaluate the dataset extracted from 1H NMR spectra of the coffee samples, result in the disclosure of the differences in the chemical profiles between the Gayo roasted coffees of arabica and robusta. Score plots obtained from the models of principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLSDA), classified the roasted coffee samples based on their species. Loading plot and S-plot revealed the discriminant compounds for each coffee. Gayo roasted arabica coffee was characterized with acetic acid and trigonelline, while the robusta coffee was discriminated with fatty acids. This report revealed the chemical differences of both coffees and confirmed the diversity of Gayo coffees.
{"title":"Discrimination of Metabolite Profiles of Gayo Roasted Arabica and Robusta Coffees","authors":"N. Happyana, Y. M. Syah, E. Hakim","doi":"10.20884/1.jm.2022.17.1.5603","DOIUrl":"https://doi.org/10.20884/1.jm.2022.17.1.5603","url":null,"abstract":"Gayo (Aceh) coffee is one of the best coffees from Indonesia. In this work, metabolites in the Gayo roasted arabica and robusta coffees were identified with 1H NMR spectroscopy analysis. Accumulatively 28 compounds were successfully detected, including the major and minor metabolites of the roasted coffee. Multivariate data analysis was applied to evaluate the dataset extracted from 1H NMR spectra of the coffee samples, result in the disclosure of the differences in the chemical profiles between the Gayo roasted coffees of arabica and robusta. Score plots obtained from the models of principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLSDA), classified the roasted coffee samples based on their species. Loading plot and S-plot revealed the discriminant compounds for each coffee. Gayo roasted arabica coffee was characterized with acetic acid and trigonelline, while the robusta coffee was discriminated with fatty acids. This report revealed the chemical differences of both coffees and confirmed the diversity of Gayo coffees.","PeriodicalId":18773,"journal":{"name":"Molekul","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43460457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.20884/1.jm.2022.17.1.5595
I. W. T. Aryasa, I. Noviandri
Paracetamol is a commom analgesic and antipyretic drug which used for reliefing fever and head ache. The determination of paracetamol dose in pharmaceuticals is very important, becauce an overdose can cause fulminating hepatic necrosis and other toxic effects. Therefore, it is necessary to measure the dose of paracetamol for the patient with precision to avoid harm. Many analytical methodologies have been proposed for determination of paracetamol dose. One of the methods was developed in the past two decades. Generally, electroanalytical approach especially voltammetry method is particularly design for determination of paracetamol dose especially in modifying electrode. This study aims to modified carbon paste electrode with molecularly imprinted polymer (MIP). Significant advantages of using MIP are the superior stability, low cost and ease of preparation. The poly (3-aminiophenol) film was prepared by cyclic voltammetry method and 3-aminophenol monomer in supporting electrolyte (HClO4) with and whitout presence of paracetamol molecule. The effect of paracetamol was seen at cyclic voltammogram was founded, where oxidation peak potential of poly (3-aminophenol) shifted to more cathodic potentials from 0.948 to 0.780 V, in presence of paracetamol. The Ipa showed a good linear relationship with concentration in the range 0.01–0.1 mM, and the detection limit was 4,63 μM.
{"title":"Voltammetric Determination of Paracetamol with Carbon Paste Electrode Modified with Molecularly Imprinted Electropolymer","authors":"I. W. T. Aryasa, I. Noviandri","doi":"10.20884/1.jm.2022.17.1.5595","DOIUrl":"https://doi.org/10.20884/1.jm.2022.17.1.5595","url":null,"abstract":"Paracetamol is a commom analgesic and antipyretic drug which used for reliefing fever and head ache. The determination of paracetamol dose in pharmaceuticals is very important, becauce an overdose can cause fulminating hepatic necrosis and other toxic effects. Therefore, it is necessary to measure the dose of paracetamol for the patient with precision to avoid harm. Many analytical methodologies have been proposed for determination of paracetamol dose. One of the methods was developed in the past two decades. Generally, electroanalytical approach especially voltammetry method is particularly design for determination of paracetamol dose especially in modifying electrode. This study aims to modified carbon paste electrode with molecularly imprinted polymer (MIP). Significant advantages of using MIP are the superior stability, low cost and ease of preparation. The poly (3-aminiophenol) film was prepared by cyclic voltammetry method and 3-aminophenol monomer in supporting electrolyte (HClO4) with and whitout presence of paracetamol molecule. The effect of paracetamol was seen at cyclic voltammogram was founded, where oxidation peak potential of poly (3-aminophenol) shifted to more cathodic potentials from 0.948 to 0.780 V, in presence of paracetamol. The Ipa showed a good linear relationship with concentration in the range 0.01–0.1 mM, and the detection limit was 4,63 μM.","PeriodicalId":18773,"journal":{"name":"Molekul","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44297076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.20884/1.jm.2022.17.1.5571
H. Kristianto, Joshua Alexander, S. Prasetyo, A. K. Sugih
Iron oxide nanoparticles, such as Fe3O4, are commonly used in various applications, such as drug delivery, magnetic fluid, water purification, and enzyme immobilization. These applications require protein to be adsorbed on the surface of iron oxide nanoparticles. However direct utilization of iron oxide nanoparticles has several drawbacks, thus modification of iron oxide nanoparticles is usually done. In this study, we reported surface modification of Fe3O4 using tannic acid to adsorb bovine serum albumin (BSA). The effect of modification and the BSA adsorption on the Fe3O4 was characterized using Fourier Transform Infrared Spectroscopy, X-ray Diffraction, Scanning Electron Microscopy, and Transmission Electron Microscopy. Furthermore the adsorption performance of modified and unmodified Fe3O4 was investigated at various initial pH, BSA concentration, and adsorption temperature. Several kinetic and isotherm adsorption models were used to describe the adsorption in this study. It was found that highest BSA adsorption was obtained at pH 4.8, near BSA’s isoelectric point. The adsorption kinetics followed the pseudo 2nd order model and reached equilibrium after 210 min adsorption. Based on Langmuir monolayer adsorption capacity, it was found that tannic acid modified Fe3O4 had 5 times higher adsorption capacity (222 mg/g), compared to unmodified Fe3O4 (46 mg/g). Furthermore, from the thermodynamic study, it was suggested that the BSA adsorption was endothermic, spontaneous, and random in nature.
{"title":"Tannic acid modified iron oxide nanoparticles and its application in protein adsorption: isotherm, kinetic, and thermodynamic study","authors":"H. Kristianto, Joshua Alexander, S. Prasetyo, A. K. Sugih","doi":"10.20884/1.jm.2022.17.1.5571","DOIUrl":"https://doi.org/10.20884/1.jm.2022.17.1.5571","url":null,"abstract":"Iron oxide nanoparticles, such as Fe3O4, are commonly used in various applications, such as drug delivery, magnetic fluid, water purification, and enzyme immobilization. These applications require protein to be adsorbed on the surface of iron oxide nanoparticles. However direct utilization of iron oxide nanoparticles has several drawbacks, thus modification of iron oxide nanoparticles is usually done. In this study, we reported surface modification of Fe3O4 using tannic acid to adsorb bovine serum albumin (BSA). The effect of modification and the BSA adsorption on the Fe3O4 was characterized using Fourier Transform Infrared Spectroscopy, X-ray Diffraction, Scanning Electron Microscopy, and Transmission Electron Microscopy. Furthermore the adsorption performance of modified and unmodified Fe3O4 was investigated at various initial pH, BSA concentration, and adsorption temperature. Several kinetic and isotherm adsorption models were used to describe the adsorption in this study. It was found that highest BSA adsorption was obtained at pH 4.8, near BSA’s isoelectric point. The adsorption kinetics followed the pseudo 2nd order model and reached equilibrium after 210 min adsorption. Based on Langmuir monolayer adsorption capacity, it was found that tannic acid modified Fe3O4 had 5 times higher adsorption capacity (222 mg/g), compared to unmodified Fe3O4 (46 mg/g). Furthermore, from the thermodynamic study, it was suggested that the BSA adsorption was endothermic, spontaneous, and random in nature.","PeriodicalId":18773,"journal":{"name":"Molekul","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44992028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.20884/1.jm.2022.17.1.5594
Dini Oktaviani, Winda Sukmawati, K. Farabi, D. Harneti, N. Nurlelasari, Darwati Darwati, R. Maharani, T. Mayanti, A. Safari, U. Supratman
Aglaia is the largest genus of the Meliaceae family which contains terpenoid compounds. This type of compounds showed a diverse structures and biological activities that can be found in natural resources. Aglaia elaeagnoidea is a species from Aglaia genus that only has a few previous research. This study was aimed to isolate and determine the chemical structure of terpenoid compounds from the ethyl acetate extract of A. elaeagnoidea stem bark. Ethyl acetate extracts were separated and purified by various chromatographic techniques to obtain compounds 1-5. Compounds 1-5 were identified their chemical structures by spectroscopic methods (IR, MS, and NMR) and comparison with previous reported spectral data. Compounds 1 and 2 were identified as eudesmane-type sesquiterpenoids, 5-epi-eudesm-4(15)-ene-1β,6β-diol (1) and 6α-Hydroxy-eudesm-4(15)-en-1-one (2). Compounds 3-5 were identified as dammarane-type triterpenoids, 20S,24S-epoxy-25-hydroxydammarane-3-one (3), 20S,24S-epoxydammarane-3α,25-diol (4), and 3α-epi-cabraleahydroxy lactone (5). These compounds are first time reported from this plant. Compounds 1-5 were tested for cytotoxicity against HeLa cervical cancer cell and DU145 prostate cancer cell and as a result, compound 4 (20S,24S-epoxydammarane-3α,25-diol) showed the stronger activity compared to other compounds.
{"title":"Terpenoids from the Stem bark of Aglaia elaeagnoidea and their cytotoxic activity against HeLa and DU145 Cancer Cell lines","authors":"Dini Oktaviani, Winda Sukmawati, K. Farabi, D. Harneti, N. Nurlelasari, Darwati Darwati, R. Maharani, T. Mayanti, A. Safari, U. Supratman","doi":"10.20884/1.jm.2022.17.1.5594","DOIUrl":"https://doi.org/10.20884/1.jm.2022.17.1.5594","url":null,"abstract":"Aglaia is the largest genus of the Meliaceae family which contains terpenoid compounds. This type of compounds showed a diverse structures and biological activities that can be found in natural resources. Aglaia elaeagnoidea is a species from Aglaia genus that only has a few previous research. This study was aimed to isolate and determine the chemical structure of terpenoid compounds from the ethyl acetate extract of A. elaeagnoidea stem bark. Ethyl acetate extracts were separated and purified by various chromatographic techniques to obtain compounds 1-5. Compounds 1-5 were identified their chemical structures by spectroscopic methods (IR, MS, and NMR) and comparison with previous reported spectral data. Compounds 1 and 2 were identified as eudesmane-type sesquiterpenoids, 5-epi-eudesm-4(15)-ene-1β,6β-diol (1) and 6α-Hydroxy-eudesm-4(15)-en-1-one (2). Compounds 3-5 were identified as dammarane-type triterpenoids, 20S,24S-epoxy-25-hydroxydammarane-3-one (3), 20S,24S-epoxydammarane-3α,25-diol (4), and 3α-epi-cabraleahydroxy lactone (5). These compounds are first time reported from this plant. Compounds 1-5 were tested for cytotoxicity against HeLa cervical cancer cell and DU145 prostate cancer cell and as a result, compound 4 (20S,24S-epoxydammarane-3α,25-diol) showed the stronger activity compared to other compounds.","PeriodicalId":18773,"journal":{"name":"Molekul","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67592346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.20884/1.jm.2022.17.1.5593
Erina Hilmayanti, N. Nurlelasari, D. G. Katja, A. Hidayat, S. Fajriah, A. Darmawan, U. Supratman, M. N. Azmi, Y. Shiono
Limonoid is derivatives of triterpenoid compound that have a wide variety of structures due to various ring-opening, rearrangements, and high-degree of oxidation. Limonoid is known as compounds that have wide-range of biological activities, including anticancer activity. This research was aimed to determine the chemical structure and cytotoxic activity of limonoid in the n-hexane extract of Chisocheton lasiocarpus fruit. Dried powder of C. lasiocarpus fruit was extracted using methanol followed by fractionated using n-hexane, ethyl acetate, and n-butanol. Five azadirone-type limonoids, 6α-(acetoxy)-14β,15β-epoxyazadirone (1), dysobinin (2), 7α-acetylneotricilenone (3), 6α-O-acetyl-7-deacetylnimocinol (4), and 7α-hyroxyneotricilenone (5), were isolated from the n-hexane extract of Chisocheton lasiocarpus fruit. The chemical structure of all compounds was identified by spectroscopic analysis, including 1D, 2D-NMR, IR, UV and HR-TOF MS as well as by comparison with previous reported spectra data. Compounds 1-5 were investigated from this plant for first time. The cytotoxic activity of the isolated compounds against MCF-7 breast cancer line were examined and the results showed that 7α-hyroxyneotricilenone (5) showed the moderate activity with IC50 values of 53 μM
类柠檬酮是三萜化合物的衍生物，由于开环、重排和高度氧化，具有多种结构。类柠檬素是一种具有广泛生物活性的化合物，包括抗癌活性。本研究的目的是测定荔枝果正己烷提取物中类柠檬素的化学结构和细胞毒活性。用甲醇提取香果干粉，再用正己烷、乙酸乙酯和正丁醇进行分馏。从Chisocheton lasiocarpus果实正己烷提取物中分离得到6α-(乙酰氧基)-14β、15β-环氧氮杂二酮(1)、dysobinin(2)、7α-乙酰新三烯酮(3)、6α- o -乙酰-7-去乙酰硝基苯二酚(4)和7α-羟基新三烯酮(5)5种氮杂二酮型柠檬酮。所有化合物的化学结构通过光谱分析，包括1D, 2D-NMR, IR, UV和HR-TOF MS，并与先前报道的光谱数据进行比较。化合物1 ~ 5为首次从该植物中分离得到。结果表明，7α-羟基新三烯酮(5)对MCF-7乳腺癌具有中等的细胞毒活性，IC50值为53 μM
{"title":"Azadirone-Type Limonoids from the Fruit of Chisocheton lasiocarpus and Their Cytotoxic Activity Against MCF-7 Breast Cancer Lines","authors":"Erina Hilmayanti, N. Nurlelasari, D. G. Katja, A. Hidayat, S. Fajriah, A. Darmawan, U. Supratman, M. N. Azmi, Y. Shiono","doi":"10.20884/1.jm.2022.17.1.5593","DOIUrl":"https://doi.org/10.20884/1.jm.2022.17.1.5593","url":null,"abstract":"Limonoid is derivatives of triterpenoid compound that have a wide variety of structures due to various ring-opening, rearrangements, and high-degree of oxidation. Limonoid is known as compounds that have wide-range of biological activities, including anticancer activity. This research was aimed to determine the chemical structure and cytotoxic activity of limonoid in the n-hexane extract of Chisocheton lasiocarpus fruit. Dried powder of C. lasiocarpus fruit was extracted using methanol followed by fractionated using n-hexane, ethyl acetate, and n-butanol. Five azadirone-type limonoids, 6α-(acetoxy)-14β,15β-epoxyazadirone (1), dysobinin (2), 7α-acetylneotricilenone (3), 6α-O-acetyl-7-deacetylnimocinol (4), and 7α-hyroxyneotricilenone (5), were isolated from the n-hexane extract of Chisocheton lasiocarpus fruit. The chemical structure of all compounds was identified by spectroscopic analysis, including 1D, 2D-NMR, IR, UV and HR-TOF MS as well as by comparison with previous reported spectra data. Compounds 1-5 were investigated from this plant for first time. The cytotoxic activity of the isolated compounds against MCF-7 breast cancer line were examined and the results showed that 7α-hyroxyneotricilenone (5) showed the moderate activity with IC50 values of 53 μM","PeriodicalId":18773,"journal":{"name":"Molekul","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47175837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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