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Dextran: Sources, Structures, and Properties 右旋糖酐:来源、结构和属性
Pub Date : 2021-07-01 DOI: 10.3390/POLYSACCHARIDES2030033
E. Díaz-Montes
Dextran is an exopolysaccharide (EPS) synthesized by lactic acid bacteria (LAB) or their enzymes in the presence of sucrose. Dextran is composed of a linear chain of d-glucoses linked by α-(1→6) bonds, with possible branches of d-glucoses linked by α-(1→4), α-(1→3), or α-(1→2) bonds, which can be low (<40 kDa) or high molecular weight (>40 kDa). The characteristics of dextran in terms of molecular weight and branches depend on the producing strain, so there is a great variety in its properties. Dextran has commercial interest because its solubility, viscosity, and thermal and rheological properties allow it to be used in food, pharmaceutical, and research areas. The aim of this review article is to compile the latest research (in the past decade) using LAB to synthesize high or low molecular weight dextran. In addition, studies using modified enzymes to produce dextran with specific structural characteristics (molecular weights and branches) are addressed. On the other hand, special attention is paid to LAB extracted from unconventional sources to expose their capacities as dextran producers and their possible application to compete with the only commercial strain (Leuconostoc mesenteroides NRRL B512).
右旋糖酐是乳酸菌(LAB)或其酶在蔗糖存在下合成的一种外多糖(EPS)。右旋糖酐是由一个由α-(1→6)键连接的线性d-葡萄糖链组成的,可能的d-葡萄糖分支由α-(1→4),α-(1→3)或α-(1→2)键连接,可以低(40 kDa)。右旋糖酐在分子量和支链方面的特性取决于生产菌株,因此其性质有很大的变化。右旋糖酐具有商业价值,因为它的溶解度、粘度、热学和流变性能使它可以用于食品、制药和研究领域。本文综述了近十年来利用LAB合成高分子量或低分子量葡聚糖的最新研究进展。此外,还讨论了利用修饰酶生产具有特定结构特征(分子量和分支)的葡聚糖的研究。另一方面,特别关注从非常规来源提取的乳酸菌,以揭示其作为葡聚糖生产者的能力,以及它们与唯一的商业菌株(Leuconostoc mesenteroides NRRL B512)竞争的可能应用。
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引用次数: 71
Rheological and Microstructural Features of Plant Culture Media Doped with Biopolymers: Influence on the Growth and Physiological Responses of In Vitro-Grown Shoots of Thymus lotocephalus 掺有生物聚合物的植物培养基流变学和微观结构特性:对胸腺离体芽生长和生理反应的影响
Pub Date : 2021-06-17 DOI: 10.3390/POLYSACCHARIDES2020032
N. Coelho, Alexandra Filipe, B. Medronho, Solange Magalhães, C. Vitorino, L. F. Alves, S. Gonçalves, A. Romano
In vitro culture is an important biotechnological tool in plant research and an appropriate culture media is a key for a successful plant development under in vitro conditions. The use of natural compounds to improve culture media has been growing and biopolymers are interesting alternatives to synthetic compounds due to their low toxicity, biodegradability, renewability, and availability. In the present study, different culture media containing one biopolymer (chitosan, gum arabic) or a biopolymer derivative [hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC)], at 100 or 1000 mg L−1, were tested regarding their influence on the growth and physiological responses of Thymus lotocephalus in vitro culture. Cellulose-based biopolymers (HEC and CMC) and gum arabic were used for the first time in plant culture media. The results showed that CMC at 100 mg L−1 significantly improved shoot elongation while chitosan, at the highest concentration, was detrimental to T. lotocephalus. Concerning only the evaluated physiological parameters, all tested biopolymers and biopolymer derivatives are safe to plants as there was no evidence of stress-induced changes on T. lotocephalus. The rheological and microstructural features of the culture media were assessed to understand how the biopolymers and biopolymer derivatives added to the culture medium could influence shoot growth. As expected, all media presented a gel-like behaviour with minor differences in the complex viscosity at the beginning of the culture period. Most media showed increased viscosity overtime. The surface area increased with the addition of biopolymers and biopolymer derivatives to the culture media and the average pore size was considerably lower for CMC at 100 mg L−1. The smaller pores of this medium might be related to a more efficient nutrients and water uptake by T. lotocephalus shoots, leading to a significant improvement in shoot elongation. In short, this study demonstrated that the different types of biopolymers and biopolymer derivatives added to culture medium can modify their microstructure and at the right concentrations, are harmless to T. lotocephalus shoots growing in vitro, and that CMC improves shoot length.
离体培养是植物研究中重要的生物技术手段,合适的培养基是植物在离体条件下发育成功的关键。使用天然化合物来改善培养基已经越来越多,生物聚合物由于其低毒、可生物降解、可再生和可用性而成为合成化合物的有趣替代品。在本研究中,研究了含有一种生物聚合物(壳聚糖、阿拉伯胶)或一种生物聚合物衍生物[羟乙基纤维素(HEC)、羧甲基纤维素(CMC)]的不同培养基,在100或1000 mg L−1浓度下,对离体培养胸腺生长和生理反应的影响。纤维素基生物聚合物(HEC和CMC)和阿拉伯胶首次用于植物培养基。结果表明,CMC在100 mg L−1浓度下显著提高了芽伸长,而壳聚糖在最高浓度下对小头天竺葵的芽伸长不利。仅就评估的生理参数而言,所有测试的生物聚合物和生物聚合物衍生物对植物都是安全的,因为没有证据表明生物聚合物和生物聚合物衍生物对小头莲有应激引起的变化。对培养基的流变学和微观结构特征进行了评估,以了解添加到培养基中的生物聚合物和生物聚合物衍生物如何影响芽的生长。正如预期的那样,在培养开始时,所有培养基都表现出凝胶样行为,在复合粘度方面存在微小差异。大多数介质随着时间的推移粘度增加。随着生物聚合物和生物聚合物衍生物的加入,培养液的表面积增加,CMC在100 mg L−1时的平均孔径明显减小。该培养基的气孔越小,可能与小头莲芽对养分和水分的吸收效率越高有关,从而显著提高了小头莲芽的伸长率。总之,本研究表明,在培养基中添加不同类型的生物聚合物及其衍生物可以改变其微观结构,并且在适当的浓度下,对离体生长的小头莲芽无害,CMC可以提高小头莲芽的长度。
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引用次数: 6
Removal of Lead, Cadmium, and Aluminum Sulfate from Simulated and Real Water with Native and Oxidized Starches 用天然和氧化淀粉从模拟和真实水中去除铅、镉和硫酸铝
Pub Date : 2021-06-04 DOI: 10.3390/POLYSACCHARIDES2020027
Konstantin B. L. Borchert, Rahma Boughanmi, Berthold Reis, Philipp Zimmermann, Christine Steinbach, Peter Graichen, Anastasiya Svirepa, J. Schwarz, R. Boldt, S. Schwarz, M. Mertig, Dana Schwarz
The separation of toxic pollutants such as Pb2+, Cd2+, and Al3+ from water is a constant challenge as contamination of natural water bodies is increasing. Al3+ and especially Pb2+ and Cd2+ are ecotoxic and highly toxic for humans, even in ppb concentrations, and therefore removal below a dangerous level is demanding. Herein, the potential adsorber material starch, being ecofriendly, cheap, and abundantly available, was investigated. Thus, four different native starch samples (potato, corn, waxy corn, and wheat starch) and two oxidized starches (oxidized potato and corn starch) were comprehensively analyzed with streaming potential and charge density measurements, SEM-EDX, ATR-FTIR, 1H-NMR, and TGA. Subsequently, the starch samples were tested for the adsorption of Pb2+, Cd2+, and Al3+ from the respective sulfate salt solution. The adsorption process was analyzed by ICP-OES and SEM-EDX, and the adsorption isotherms were fitted comparing Langmuir, Sips, and Dubinin-Radushkevich models. Oxidized starch, for which chemical modification is one of the simplest, and also native potato starch were excellent natural adsorber materials for Al3+, Cd2+, and especially Pb2+ in the low concentration range, exhibiting maximum adsorption capacities of 84, 71, and 104 µmol/g for oxidized potato starch, respectively.
随着天然水体污染的不断增加,从水体中分离Pb2+、Cd2+、Al3+等有毒污染物是一个持续的挑战。Al3+,特别是Pb2+和Cd2+对人类具有生态毒性和高度毒性,即使在ppb浓度下也是如此,因此需要将其去除到危险水平以下。在此,研究了一种生态友好、价格低廉、资源丰富的有潜力的吸附材料淀粉。因此,对四种不同的天然淀粉样品(马铃薯、玉米、糯玉米和小麦淀粉)和两种氧化淀粉(氧化马铃薯和玉米淀粉)进行了综合分析,采用了流电位和电荷密度测量、SEM-EDX、ATR-FTIR、1H-NMR和TGA。随后,测试了淀粉样品对不同硫酸盐溶液中Pb2+、Cd2+和Al3+的吸附。采用ICP-OES和SEM-EDX分析了吸附过程,并拟合了Langmuir、Sips和Dubinin-Radushkevich模型的吸附等温线。氧化淀粉是化学改性最简单的方法之一,马铃薯淀粉是Al3+、Cd2+,尤其是Pb2+的天然吸附材料,在低浓度范围内,氧化马铃薯淀粉的最大吸附量分别为84、71和104µmol/g。
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引用次数: 6
Polysaccharide-Based Packaging Functionalized with Inorganic Nanoparticles for Food Preservation 无机纳米颗粒功能化的多糖基食品保鲜包装
Pub Date : 2021-05-30 DOI: 10.3390/POLYSACCHARIDES2020026
L. M. Anaya-Esparza, Zuamí Villagrán-de la Mora, Noé Rodríguez-Barajas, J. M. Ruvalcaba-Gómez, Laura Elena Iñiguez-Muñoz, C. I. Maytorena-Verdugo, E. Montalvo-González, A. Pérez-Larios
Functionalization of polysaccharide-based packaging incorporating inorganic nanoparticles for food preservation is an active research area. This review summarizes the use of polysaccharide-based materials functionalized with inorganic nanoparticles (TiO2, ZnO, Ag, SiO2, Al2O3, Fe2O3, Zr, MgO, halloysite, and montmorillonite) to develop hybrid packaging for fruit, vegetables, meat (lamb, minced, pork, and poultry), mushrooms, cheese, eggs, and Ginkgo biloba seeds preservation. Their effects on quality parameters and shelf life are also discussed. In general, treated fruit, vegetables, mushrooms, and G. biloba seeds markedly increased their shelf life without significant changes in their sensory attributes, associated with a slowdown effect in the ripening process (respiration rate) due to the excellent gas exchange and barrier properties that effectively prevented dehydration, weight loss, enzymatic browning, microbial infections by spoilage and foodborne pathogenic bacteria, and mildew apparition in comparison with uncoated or polysaccharide-coated samples. Similarly, hybrid packaging showed protective effects to preserve meat products, cheese, and eggs by preventing microbial infections and lipid peroxidation, extending the food product’s shelf life without changes in their sensory attributes. According to the evidence, polysaccharide-hybrid packaging can preserve the quality parameters of different food products. However, further studies are needed to guarantee the safe implementation of these organic–inorganic packaging materials in the food industry.
含无机纳米颗粒的多糖基包装在食品保鲜中的功能化是一个活跃的研究领域。本文综述了利用无机纳米粒子(TiO2、ZnO、Ag、SiO2、Al2O3、Fe2O3、Zr、MgO、高岭土和蒙脱土)功能化的多糖基材料开发水果、蔬菜、肉类(羊肉、肉末、猪肉和家禽)、蘑菇、奶酪、鸡蛋和银杏种子保鲜复合包装的研究进展。讨论了它们对质量参数和保质期的影响。总的来说,经过处理的水果、蔬菜、蘑菇和双叶菌种子在没有显著改变其感官属性的情况下显著延长了它们的保质期,这与成熟过程中的减缓效应(呼吸速率)有关,因为它们具有出色的气体交换和屏障特性,有效地防止了脱水、体重减轻、酶促褐变、腐败引起的微生物感染和食源性致病菌。与未包被或包被多糖的样品相比,霉菌的出现。同样,混合包装通过防止微生物感染和脂质过氧化,延长食品的保质期而不改变其感官属性,显示出保存肉制品、奶酪和鸡蛋的保护作用。实验结果表明,多糖复合包装能有效地保存不同食品的质量参数。然而,需要进一步的研究来保证这些有机-无机包装材料在食品工业中的安全实施。
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引用次数: 6
Control of Surface Properties of Hyaluronan/Chitosan Multilayered Coatings for Tumor Cell Capture 透明质酸/壳聚糖多层膜对肿瘤细胞捕获的表面特性控制
Pub Date : 2021-05-30 DOI: 10.3390/POLYSACCHARIDES2020025
Giulia Grassa Lima, J. R. Rocha Neto, H. F. Carvalho, M. Beppu
Prostate cancer (PCa) is a slow-growing neoplasm that has, when diagnosed in its early stages, great chances of cure. During initial tumor development, current diagnostic methods fail to have the desired accuracy, thus, it is necessary to develop or improve current detection methods and prognostic markers for PCa. In this scenario, films composed of hyaluronic acid (HA) and chitosan (CHI) have demonstrated significant capture potential of prostate tumor cells (PC3 line), exploring HA as a CD44 receptor ligand and direct mediator in cell-film adhesion. Here, we present a strategy to control structural and cell adhesion properties of HA/CHI films based on film assembly conditions. Films were built via Layer-by-layer (LbL) deposition, where the pH conditions (3.0 and 5.0) and number of bilayers (3.5, 10.5, and 20.5) were controlled. The characterization of these films was carried out using profilometry, ultraviolet-visible (UV-VIS), atomic force microscopy (AFM) and contact angle measurements. Multilayer HA/CHI films produced at pH 3.0 gave optimum surface wettability and availability of free carboxyl groups. In turn, at pH 5.0, the coverings were thinner and presented a smoother surface. Films prepared with 3.5 bilayers showed greater tumor cell capture regardless of the pH condition, while films containing 10.5 and 20.5 bilayers presented a significant swelling process, which compromised their cell adhesion potential. This study shows that surface chemistry and morphology are critical factors for the development of biomaterials designed for several cell adhesion applications, such as rapid diagnostic, cell signaling, and biosensing mechanisms.
前列腺癌(PCa)是一种生长缓慢的肿瘤,如果在早期阶段被诊断出来,治愈的机会很大。在肿瘤发展初期,现有的诊断方法无法达到预期的准确性,因此有必要开发或改进现有的前列腺癌检测方法和预后标志物。在这种情况下,透明质酸(HA)和壳聚糖(CHI)组成的膜显示了前列腺肿瘤细胞(PC3系)的显著捕获潜力,探索了HA作为CD44受体配体和细胞膜粘附的直接介质。在这里,我们提出了一种基于膜组装条件来控制HA/CHI膜的结构和细胞粘附性能的策略。在控制pH值(3.0和5.0)和双层膜数量(3.5、10.5和20.5)的条件下,通过逐层沉积(LbL)法制备薄膜。利用轮廓法、紫外-可见(UV-VIS)、原子力显微镜(AFM)和接触角测量对这些薄膜进行了表征。pH为3.0时制备的多层HA/CHI膜具有最佳的表面润湿性和游离羧基的可用性。反过来,在pH 5.0时,覆盖层更薄,表面更光滑。无论pH值如何,3.5双分子层制备的膜都表现出更大的肿瘤细胞捕获,而10.5和20.5双分子层制备的膜则表现出明显的肿胀过程,损害了其细胞粘附潜力。这项研究表明,表面化学和形态是开发用于多种细胞粘附应用的生物材料的关键因素,如快速诊断、细胞信号传导和生物传感机制。
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引用次数: 3
Edible Films and Coatings Formulated with Arrowroot Starch as a Non-Conventional Starch Source for Plums Packaging 以竹淀粉为非传统淀粉源配制梅子包装用可食性薄膜和涂料
Pub Date : 2021-05-27 DOI: 10.3390/POLYSACCHARIDES2020024
Gislaine Ferreira Nogueira, Bianca de Oliveira Leme, G. Santos, J. A. D. Silva, P. Nascimento, C. Soares, F. Fakhouri, R. D. de Oliveira
Increasing environmental awareness has promoted an interest in alternative strategies to common plastics obtained from fossil sources, stimulating research on the use of biodegradable and edible films/coatings obtained from renewable sources such as arrowroot starch. This research work aimed to evaluate the use of arrowroot starch on the formation of edible films and coatings. Increasing the concentration of arrowroot starch (from 1% to 5%, mass/mass) in the film produced by casting resulted in increased water vapor permeability (from 2.20 to 3.68 g mm/m2 day kPa), moisture content (3.22% to 7.95%), increased thickness (from 0.029 to 0.101 mm), and decreased solubility in water (from 22.45% to 13.89%). The films were homogeneous, transparent and manageable, with the exception of the film with 1% starch. Film-forming solutions at concentrations of 0%, 2%, and 4% (mass/mass) of arrowroot starch were prepared and applied to plums to evaluate post-harvest behavior when stored at 25 and 5 °C for 35 days. The 2% coating adhered well to the plums’ surfaces, was bright and was effective in reducing mass loss and respiratory rate, associated with storage temperature of 5 °C. The 4% coating presented an opaque and flocculated appearance.
日益提高的环境意识促进了人们对从化石来源获得的普通塑料的替代战略的兴趣,刺激了对使用从可再生来源(如竹淀粉)获得的可生物降解和可食用薄膜/涂层的研究。本研究旨在评价竹淀粉在可食性薄膜和涂层形成中的应用。提高竹粉在铸膜中的浓度(从1%增加到5%,质量/质量),可使膜的水蒸气渗透性(从2.20增加到3.68 g mm/m2 day kPa)、含水率(从3.22%增加到7.95%)、膜厚(从0.029增加到0.101 mm)和溶解度(从22.45%减少到13.89%)增加。除了含有1%淀粉的膜外,膜均质,透明且易于管理。制备了浓度为0%、2%和4%(质量/质量)的竹芋淀粉成膜溶液,并将其应用于李子,在25°C和5°C下储存35天,以评估收获后的行为。在5°C的贮藏温度下,2%的涂层能很好地附着在李子表面,且色泽明亮,能有效地降低质量损失和呼吸速率。4%的涂层呈不透明和絮凝状。
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引用次数: 9
Marine Polysaccharides: Properties and Applications 海洋多糖:性质与应用
Pub Date : 2021-05-24 DOI: 10.1002/9781119711414.CH20
O. Michael, C. Adetunji, A. Ayeni, M. Akram, Inamuddin, J. B. Adetunji, M. Olaniyan, M. Muhibi
{"title":"Marine Polysaccharides: Properties and Applications","authors":"O. Michael, C. Adetunji, A. Ayeni, M. Akram, Inamuddin, J. B. Adetunji, M. Olaniyan, M. Muhibi","doi":"10.1002/9781119711414.CH20","DOIUrl":"https://doi.org/10.1002/9781119711414.CH20","url":null,"abstract":"","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72756137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Alginates: Properties and Applications 海藻酸盐:性质和应用
Pub Date : 2021-05-24 DOI: 10.1002/9781119711414.CH19
S. Raghav, P. Jain, Dinesh Kumar
{"title":"Alginates: Properties and Applications","authors":"S. Raghav, P. Jain, Dinesh Kumar","doi":"10.1002/9781119711414.CH19","DOIUrl":"https://doi.org/10.1002/9781119711414.CH19","url":null,"abstract":"","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82746917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Marine Polysaccharides: Properties and Applications 海洋多糖:性质与应用
Pub Date : 2021-05-17 DOI: 10.1002/9781119711414.CH3
Tonmoy Ghosh, Rabinder Singh, A. A. Nesamma, P. P. Jutur
{"title":"Marine Polysaccharides: Properties and Applications","authors":"Tonmoy Ghosh, Rabinder Singh, A. A. Nesamma, P. P. Jutur","doi":"10.1002/9781119711414.CH3","DOIUrl":"https://doi.org/10.1002/9781119711414.CH3","url":null,"abstract":"","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74078935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell Wall Polysaccharides 细胞壁多糖
Pub Date : 2021-05-17 DOI: 10.1002/9781119711414.CH2
A. Ullah, Lutufur Rahman, Muhammad Bilal Yazdani, Muhammad Irfan, Waheed S. Khan, A. Rehman
{"title":"Cell Wall Polysaccharides","authors":"A. Ullah, Lutufur Rahman, Muhammad Bilal Yazdani, Muhammad Irfan, Waheed S. Khan, A. Rehman","doi":"10.1002/9781119711414.CH2","DOIUrl":"https://doi.org/10.1002/9781119711414.CH2","url":null,"abstract":"","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"714 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86610557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Natural Polysaccharides in Drug Delivery and Biomedical Applications
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