Pub Date : 2022-04-29DOI: 10.3390/polysaccharides3020022
Pricila Veiga-Santos, Karina de Jesus Antonio, C. Santos, Amanda Alves Arruda, Larissa Bindo de Barros, Larissa Tulio Gonçalves
Fruit and vegetable-based materials, rich in phenolic pigments, and especially anthocyanins, have attracted attention as promising sources for bio-based antioxidant coating polymers, being a non-toxic, natural, ecofriendly, and green label solution to lower oxidation degradation in oil-water emulsion food, such as cheeses. However, could their pomaces also be used in such materials? This work has investigated the use of jabuticaba peels and red cabbage stir pomace extracts as antioxidant additives for cheese coating polymers. The antioxidant capacity of the jabuticaba-red cabbage pomace cassava-based polymer was evaluated in vitro (total phenolic, total anthocyanin content and DPPH scavenging %) and in vivo (by coating Minas Frescal cheeses and monitoring their peroxide index increase during a 9-day shelf life, at 10 °C). An in vitro characterization has indicated a high antioxidant capacity for both pomace extracts, with a higher capacity observed for the jabuticaba peels. In vivo investigations indicated that the pomace-starch coatings have protected cheeses up to 8.5 times against oxidation when compared to the control, with a synergistic protector effect among pomaces. Physical–chemical characterizations (pH, acidity, total solids, ash, total protein, fat content and syneresis) have indicated no coating interference on the cheese’s development.
{"title":"Pomace-Cassava as Antioxidant Bio-Based Coating Polymers for Cheeses","authors":"Pricila Veiga-Santos, Karina de Jesus Antonio, C. Santos, Amanda Alves Arruda, Larissa Bindo de Barros, Larissa Tulio Gonçalves","doi":"10.3390/polysaccharides3020022","DOIUrl":"https://doi.org/10.3390/polysaccharides3020022","url":null,"abstract":"Fruit and vegetable-based materials, rich in phenolic pigments, and especially anthocyanins, have attracted attention as promising sources for bio-based antioxidant coating polymers, being a non-toxic, natural, ecofriendly, and green label solution to lower oxidation degradation in oil-water emulsion food, such as cheeses. However, could their pomaces also be used in such materials? This work has investigated the use of jabuticaba peels and red cabbage stir pomace extracts as antioxidant additives for cheese coating polymers. The antioxidant capacity of the jabuticaba-red cabbage pomace cassava-based polymer was evaluated in vitro (total phenolic, total anthocyanin content and DPPH scavenging %) and in vivo (by coating Minas Frescal cheeses and monitoring their peroxide index increase during a 9-day shelf life, at 10 °C). An in vitro characterization has indicated a high antioxidant capacity for both pomace extracts, with a higher capacity observed for the jabuticaba peels. In vivo investigations indicated that the pomace-starch coatings have protected cheeses up to 8.5 times against oxidation when compared to the control, with a synergistic protector effect among pomaces. Physical–chemical characterizations (pH, acidity, total solids, ash, total protein, fat content and syneresis) have indicated no coating interference on the cheese’s development.","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88369136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-26DOI: 10.3390/polysaccharides3020021
Karina Haro Carrasco, Egon Götz Höfgen, D. Brunner, Konstantin B. L. Borchert, Berthold Reis, Christine Steinbach, M. Mayer, S. Schwarz, K. Glas, Dana Schwarz
The human-made pollution of surface and ground waters is becoming an inevitable and persistently urgent problem for humankind and life in general, as these pollutants are also distributed by their natural circulation. For example, from mining activities and metallurgy, toxic heavy metals pollute the environment and present material risk for human health and the environment. Bioadsorbers are an intriguing way to efficiently capture and eliminate these hazards, as they are environmentally friendly, cheap, abundant, and efficient. In this study, we present brewers’ spent grain (BSG) as an efficient adsorber for toxic heavy metal ions, based on the examples of iron, manganese, cadmium, and nickel ions. We uncover the adsorption properties of two different BSGs and investigate thoroughly their chemical and physical properties as well as their efficiency as adsorbers for simulated and real surface waters. As a result, we found that the adsorption behavior of BSG types differs despite almost identical chemistry. Elemental mapping reveals that all components of BSG contribute to the adsorption. Further, both types are not only able to purify water to reach acceptable levels of cleanness, but also yield outstanding adsorption performance for iron ions of 0.2 mmol/g and for manganese, cadmium, and nickel ions of 0.1 mmol/g.
{"title":"Removal of Iron, Manganese, Cadmium, and Nickel Ions Using Brewers’ Spent Grain","authors":"Karina Haro Carrasco, Egon Götz Höfgen, D. Brunner, Konstantin B. L. Borchert, Berthold Reis, Christine Steinbach, M. Mayer, S. Schwarz, K. Glas, Dana Schwarz","doi":"10.3390/polysaccharides3020021","DOIUrl":"https://doi.org/10.3390/polysaccharides3020021","url":null,"abstract":"The human-made pollution of surface and ground waters is becoming an inevitable and persistently urgent problem for humankind and life in general, as these pollutants are also distributed by their natural circulation. For example, from mining activities and metallurgy, toxic heavy metals pollute the environment and present material risk for human health and the environment. Bioadsorbers are an intriguing way to efficiently capture and eliminate these hazards, as they are environmentally friendly, cheap, abundant, and efficient. In this study, we present brewers’ spent grain (BSG) as an efficient adsorber for toxic heavy metal ions, based on the examples of iron, manganese, cadmium, and nickel ions. We uncover the adsorption properties of two different BSGs and investigate thoroughly their chemical and physical properties as well as their efficiency as adsorbers for simulated and real surface waters. As a result, we found that the adsorption behavior of BSG types differs despite almost identical chemistry. Elemental mapping reveals that all components of BSG contribute to the adsorption. Further, both types are not only able to purify water to reach acceptable levels of cleanness, but also yield outstanding adsorption performance for iron ions of 0.2 mmol/g and for manganese, cadmium, and nickel ions of 0.1 mmol/g.","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84318961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-06DOI: 10.3390/polysaccharides3020020
A. M. N. de Toledo, A. R. Machado, L. A. de Souza-Soares
Electrostatic complexes based on chitosan, lecithin, and sodium tripolyphosphate were produced and evaluated with respect to their encapsulation capacity and cytotoxicity. Physical chemical properties were determined by zeta potential values and size distributions. For encapsulation assays, the emulsification method was followed, and Citrus senensis peel oil was utilized as volatile compound model. Morphology of complexes with oil incorporated was observed by scanning electron microscopy. The cytotoxicity of complexes was related to cell viability of zebrafish hepatocytes. The complexes produced presented positive Zeta potential values and size distributions dependent on the mass ratio between compounds. Higher concentrations of sodium tripolyphosphate promote significant changes (p < 0.05) in zeta values, which did not occur at smaller concentrations of the crosslinking agent. These complexes were able to encapsulate Citrus sinensis peel oil, with encapsulation efficiency higher than 50%. Cytotoxicity profiles showed that in a range of concentrations (0.1–100 μg/mL) studied, they did not promote cellular damage in zebrafish liver cells, being potential materials for food and pharmaceutical applications.
{"title":"Development and In Vitro Cytotoxicity of Citrus sinensis Oil-Loaded Chitosan Electrostatic Complexes","authors":"A. M. N. de Toledo, A. R. Machado, L. A. de Souza-Soares","doi":"10.3390/polysaccharides3020020","DOIUrl":"https://doi.org/10.3390/polysaccharides3020020","url":null,"abstract":"Electrostatic complexes based on chitosan, lecithin, and sodium tripolyphosphate were produced and evaluated with respect to their encapsulation capacity and cytotoxicity. Physical chemical properties were determined by zeta potential values and size distributions. For encapsulation assays, the emulsification method was followed, and Citrus senensis peel oil was utilized as volatile compound model. Morphology of complexes with oil incorporated was observed by scanning electron microscopy. The cytotoxicity of complexes was related to cell viability of zebrafish hepatocytes. The complexes produced presented positive Zeta potential values and size distributions dependent on the mass ratio between compounds. Higher concentrations of sodium tripolyphosphate promote significant changes (p < 0.05) in zeta values, which did not occur at smaller concentrations of the crosslinking agent. These complexes were able to encapsulate Citrus sinensis peel oil, with encapsulation efficiency higher than 50%. Cytotoxicity profiles showed that in a range of concentrations (0.1–100 μg/mL) studied, they did not promote cellular damage in zebrafish liver cells, being potential materials for food and pharmaceutical applications.","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76295007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-05DOI: 10.3390/polysaccharides3020019
M. Baikousi, Konstantinos Moustaklis, Angeliki Karakassides, Georgios Asimakopoulos, D. Moschovas, Apostolos Avgeropoulos, A. Bourlinos, A. Douvalis, C. Salmas, M. Karakassides
Nowadays, the scientific interest is focused more and more on the development of new strategies in recycling of waste products as well as on the development of clean technologies due to the increased environmental pollution. In this work we studied the valorization of an expired cheese-tomato flavor corn snack, which is polysaccharide food product, by producing advanced hybrid magnetic materials for environmental remediation purposes. The carbonization-chemical activation of this snack using potassium hydroxide leads to a microporous activated carbon with high surface area (SgBET ~800 m2/g). The magnetic hybrid material was synthesized via an in-situ technique using iron acetate complex as the precursor to produce iron based magnetic nanoparticles. The resulting material retains a fraction of the microporous structure with surface area SgBET ~500 m2/g. Such material consists, of homogenously dispersed magnetic isolated zero valent iron nanoparticles and of iron carbides (Fe3C), into the carbon matrix. The magnetic carbon exhibited high adsorption capacity in Cr(VI) removal applications following a pseudosecond order kinetic model. The maximum adsorption capacity was 88.382 mgCr(VI)/gAC at pH = 3. Finally, oxidation experiments, in combination with FT-IR, Mössbauer, and VSM measurements indicated that the possible Cr6+ removal mechanism involves oxidation of iron phases and reduction of Cr6+ to Cr3+.
{"title":"Use of a Hybrid Porous Carbon Material Derived from Expired Polysaccharides Snack/Iron Salt Exhibiting Magnetic Properties, for Hexavalent Chromium Removal","authors":"M. Baikousi, Konstantinos Moustaklis, Angeliki Karakassides, Georgios Asimakopoulos, D. Moschovas, Apostolos Avgeropoulos, A. Bourlinos, A. Douvalis, C. Salmas, M. Karakassides","doi":"10.3390/polysaccharides3020019","DOIUrl":"https://doi.org/10.3390/polysaccharides3020019","url":null,"abstract":"Nowadays, the scientific interest is focused more and more on the development of new strategies in recycling of waste products as well as on the development of clean technologies due to the increased environmental pollution. In this work we studied the valorization of an expired cheese-tomato flavor corn snack, which is polysaccharide food product, by producing advanced hybrid magnetic materials for environmental remediation purposes. The carbonization-chemical activation of this snack using potassium hydroxide leads to a microporous activated carbon with high surface area (SgBET ~800 m2/g). The magnetic hybrid material was synthesized via an in-situ technique using iron acetate complex as the precursor to produce iron based magnetic nanoparticles. The resulting material retains a fraction of the microporous structure with surface area SgBET ~500 m2/g. Such material consists, of homogenously dispersed magnetic isolated zero valent iron nanoparticles and of iron carbides (Fe3C), into the carbon matrix. The magnetic carbon exhibited high adsorption capacity in Cr(VI) removal applications following a pseudosecond order kinetic model. The maximum adsorption capacity was 88.382 mgCr(VI)/gAC at pH = 3. Finally, oxidation experiments, in combination with FT-IR, Mössbauer, and VSM measurements indicated that the possible Cr6+ removal mechanism involves oxidation of iron phases and reduction of Cr6+ to Cr3+.","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"126 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90870680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-23DOI: 10.3390/polysaccharides3020018
C. Camacho-González, C. Cardona‐Félix, V. Zamora-Gasga, A. Pérez-Larios, J. Sánchez-Burgos
In the aquaculture sector, the biofunctionalization of biomaterials is discussed using materials from algae and analyzed as a possible potential strategy to overcome the challenges that hinder the future development of the application of endolysins in this field. Derived from years of analysis, endolysins have recently been considered as potential alternative therapeutic antibacterial agents, due to their attributes and ability to combat multi-resistant bacterial cells when applied externally. On the other hand, although the aquaculture sector has been characterized by its high production rates, serious infectious diseases have led to significant economic losses that persist to this day. Although there are currently interesting data from studies under in vitro conditions on the application of endolysins in this sector, there is little or no information on in vivo studies. This lack of analysis can be attributed to the relatively low stability of endolysins in marine conditions and to the complex gastrointestinal conditions of the organisms. This review provides updated information regarding the application of endolysins against multi-resistant bacteria of clinical and nutritional interest, previously addressing their important characteristics (structure, properties and stability). In addition, regarding the aquaculture sector, the biofunctionalization of biomaterials is discussed using materials from algae and analyzed as a possible potential strategy to overcome the challenges that hinder the future development of the application of endolysins in this field.
{"title":"Biofunctionalization of Endolysins with Oligosacharides: Formulation of Therapeutic Agents to Combat Multi-Resistant Bacteria and Potential Strategies for Their Application","authors":"C. Camacho-González, C. Cardona‐Félix, V. Zamora-Gasga, A. Pérez-Larios, J. Sánchez-Burgos","doi":"10.3390/polysaccharides3020018","DOIUrl":"https://doi.org/10.3390/polysaccharides3020018","url":null,"abstract":"In the aquaculture sector, the biofunctionalization of biomaterials is discussed using materials from algae and analyzed as a possible potential strategy to overcome the challenges that hinder the future development of the application of endolysins in this field. Derived from years of analysis, endolysins have recently been considered as potential alternative therapeutic antibacterial agents, due to their attributes and ability to combat multi-resistant bacterial cells when applied externally. On the other hand, although the aquaculture sector has been characterized by its high production rates, serious infectious diseases have led to significant economic losses that persist to this day. Although there are currently interesting data from studies under in vitro conditions on the application of endolysins in this sector, there is little or no information on in vivo studies. This lack of analysis can be attributed to the relatively low stability of endolysins in marine conditions and to the complex gastrointestinal conditions of the organisms. This review provides updated information regarding the application of endolysins against multi-resistant bacteria of clinical and nutritional interest, previously addressing their important characteristics (structure, properties and stability). In addition, regarding the aquaculture sector, the biofunctionalization of biomaterials is discussed using materials from algae and analyzed as a possible potential strategy to overcome the challenges that hinder the future development of the application of endolysins in this field.","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"358 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78107372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-13DOI: 10.3390/polysaccharides3010017
Jéssica F Pereira, B. M. Marim, S. Mali
Cellulose is a natural, unbranched, and fibrous homopolymer that is a major component in several agroindustrial residues. The aim of this study was to extract cellulose from oat hulls and then to modify it using a green route to obtain esterified cellulose through reaction with organic acids employing the reactive extrusion process, which is a process that presents some advantages, including low effluent generation, short reaction times, and it is scalable for large scale use. Citric (CA) and succinic (SA) acids were employed as esterifying agents in different concentrations (0, 5, 12.5, and 20%). Modified cellulose samples were characterized by their degree of substitution (DS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (DRX), scanning electron microscopy (SEM), wettability, oil and water absorption capacities, and thermal stability. DS of modified samples ranged from 2.28 to 3.00, and FTIR results showed that the esterification occurred in all samples for both acids by observation of important bands at 1720 and 1737 cm−1 for samples modified with CA and SA, respectively. All modified samples presented increased hydrophobicity. The modification did not have an influence on the morphological structure or crystallinity pattern of all samples. This study proved to be possible to modify cellulose using a simple and ecofriendly process based on reactive extrusion with organic acids.
{"title":"Chemical Modification of Cellulose Using a Green Route by Reactive Extrusion with Citric and Succinic Acids","authors":"Jéssica F Pereira, B. M. Marim, S. Mali","doi":"10.3390/polysaccharides3010017","DOIUrl":"https://doi.org/10.3390/polysaccharides3010017","url":null,"abstract":"Cellulose is a natural, unbranched, and fibrous homopolymer that is a major component in several agroindustrial residues. The aim of this study was to extract cellulose from oat hulls and then to modify it using a green route to obtain esterified cellulose through reaction with organic acids employing the reactive extrusion process, which is a process that presents some advantages, including low effluent generation, short reaction times, and it is scalable for large scale use. Citric (CA) and succinic (SA) acids were employed as esterifying agents in different concentrations (0, 5, 12.5, and 20%). Modified cellulose samples were characterized by their degree of substitution (DS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (DRX), scanning electron microscopy (SEM), wettability, oil and water absorption capacities, and thermal stability. DS of modified samples ranged from 2.28 to 3.00, and FTIR results showed that the esterification occurred in all samples for both acids by observation of important bands at 1720 and 1737 cm−1 for samples modified with CA and SA, respectively. All modified samples presented increased hydrophobicity. The modification did not have an influence on the morphological structure or crystallinity pattern of all samples. This study proved to be possible to modify cellulose using a simple and ecofriendly process based on reactive extrusion with organic acids.","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88814025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-12DOI: 10.3390/polysaccharides3010016
C. Marques, R. R. Silva, T. R. Arruda, Ana Luiza Valadares Ferreira, T. V. D. Oliveira, A. R. F. Moraes, M. V. Dias, M. Vanetti, N. Soares
The obtainment of new materials with distinct properties by mixing two or more polymers is a potential strategy in sustainable packaging research. In the present work, a blend of cellulose acetate (CA) and zein (60:40 wt/wt CA:zein) was manufactured by adding glycerol or tributyrin as plasticizers (30% wt/wt), and garlic essential oil (GEO), complexed (IC) or not with β-cyclodextrin (βCD), to produce active packaging. Blends plasticized with tributyrin exhibited a more homogeneous surface than those containing glycerol, which showed major defects. The blends underperformed compared with the CA films regarding mechanical properties and water vapor permeability. The presence of IC also impaired the films’ performance. However, the blends were more flexible than zein brittle films. The films added with GEO presented in vitro activity against Listeria innocua and Staphylococcus aureus. The IC addition into films, however, did not ensure antibacterial action, albeit that IC, when tested alone, showed activity against both bacteria. These findings suggest that the mixture of CA and plasticizers could increase the range of application of zein as a sustainable packaging component, while essential oils act as a natural bioactive to produce active packaging.
{"title":"Development and Investigation of Zein and Cellulose Acetate Polymer Blends Incorporated with Garlic Essential Oil and β-Cyclodextrin for Potential Food Packaging Application","authors":"C. Marques, R. R. Silva, T. R. Arruda, Ana Luiza Valadares Ferreira, T. V. D. Oliveira, A. R. F. Moraes, M. V. Dias, M. Vanetti, N. Soares","doi":"10.3390/polysaccharides3010016","DOIUrl":"https://doi.org/10.3390/polysaccharides3010016","url":null,"abstract":"The obtainment of new materials with distinct properties by mixing two or more polymers is a potential strategy in sustainable packaging research. In the present work, a blend of cellulose acetate (CA) and zein (60:40 wt/wt CA:zein) was manufactured by adding glycerol or tributyrin as plasticizers (30% wt/wt), and garlic essential oil (GEO), complexed (IC) or not with β-cyclodextrin (βCD), to produce active packaging. Blends plasticized with tributyrin exhibited a more homogeneous surface than those containing glycerol, which showed major defects. The blends underperformed compared with the CA films regarding mechanical properties and water vapor permeability. The presence of IC also impaired the films’ performance. However, the blends were more flexible than zein brittle films. The films added with GEO presented in vitro activity against Listeria innocua and Staphylococcus aureus. The IC addition into films, however, did not ensure antibacterial action, albeit that IC, when tested alone, showed activity against both bacteria. These findings suggest that the mixture of CA and plasticizers could increase the range of application of zein as a sustainable packaging component, while essential oils act as a natural bioactive to produce active packaging.","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74701715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-08DOI: 10.3390/polysaccharides3010015
Juliana Botelho Moreira, Suelen Goettems Kuntzler, P. Bezerra, A. P. A. Cassuriaga, M. Zaparoli, Jacinta Lutécia Vitorino da Silva, J. Costa, M. G. de Morais
Microalgae are used in flocculation processes because biopolymers are released into the culture medium. Microalgal cell growth under specific conditions (temperature, pH, luminosity, nutrients, and salinity) provides the production and release of exopolysaccharides (EPS). These biopolymers can be recovered from the medium for application as bioflocculants or used directly in cultivation as microalgae autoflocculants. The optimization of nutritional parameters, the control of process conditions, and the possibility of scaling up allow the production and industrial application of microalgal EPS. Therefore, this review addresses the potential use of EPS produced by microalgae in bioflocculation. The recovery, determination, and quantification techniques for these biopolymers are also addressed. Moreover, other technological applications of EPS are highlighted.
{"title":"Recent Advances of Microalgae Exopolysaccharides for Application as Bioflocculants","authors":"Juliana Botelho Moreira, Suelen Goettems Kuntzler, P. Bezerra, A. P. A. Cassuriaga, M. Zaparoli, Jacinta Lutécia Vitorino da Silva, J. Costa, M. G. de Morais","doi":"10.3390/polysaccharides3010015","DOIUrl":"https://doi.org/10.3390/polysaccharides3010015","url":null,"abstract":"Microalgae are used in flocculation processes because biopolymers are released into the culture medium. Microalgal cell growth under specific conditions (temperature, pH, luminosity, nutrients, and salinity) provides the production and release of exopolysaccharides (EPS). These biopolymers can be recovered from the medium for application as bioflocculants or used directly in cultivation as microalgae autoflocculants. The optimization of nutritional parameters, the control of process conditions, and the possibility of scaling up allow the production and industrial application of microalgal EPS. Therefore, this review addresses the potential use of EPS produced by microalgae in bioflocculation. The recovery, determination, and quantification techniques for these biopolymers are also addressed. Moreover, other technological applications of EPS are highlighted.","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81248678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-23DOI: 10.3390/polysaccharides3010014
Gislaine Ferreira Nogueira, I. B. Soares, C. Soares, F. Fakhouri, R. D. de Oliveira
Grape processing residues are a good source of bioactive and nutritional compounds. The incorporation of grape pomace extract (GPE) in starch films can be a strategy for the elaboration of new food packaging products for applications such as edible films or fruit strips. In this context, the objective of this research was to analyze the effect of incorporation and variation of concentration of GPE (0, 20, 30, and 40% mass/mass starch solids) on the physical chemical properties of arrowroot starch edible films created by casting. The GPE was characterized for moisture content, pH, total titratable acidity, total soluble solids, and anthocyanin content. Starch films with and without GPE were evaluated by analyzing their visual appearance, water activity, water content, thickness, water solubility, and water vapor permeability. The GPE had high water content, acidity, and anthocyanins content. The films with GPE showed a noticeable reddish color, similar to observed for the GPE. Increasing the concentration of GPE in the film resulted in significantly increased (p < 0.05) thickness (from 0.060 to 0.106 mm), water content (from 8.17 to 12.48%), solubility in water (from 13.33 to 33.32%), and water vapor permeability (from 3.72 to 6.65 g.mm/m2 day kPa). GPE increased the hydrophilic portion of the film, in addition to acting as a plasticizer, decreasing the molecular interactions of the polymer chain, and favoring its solubilization, which is desirable for applications such as edible films. The elaboration of arrowroot starch films with the incorporation of grape pomace is a good alternative for the reduction of by-products of grape processing.
葡萄加工残留物是生物活性和营养化合物的良好来源。葡萄渣提取物(GPE)在淀粉薄膜中的掺入可以作为一种策略，用于制作新的食品包装产品，如可食用薄膜或水果条。在此背景下，本研究的目的是分析GPE(0、20、30和40%质量/质量淀粉固体)掺入和浓度变化对竹芋淀粉可食用薄膜物理化学性质的影响。表征GPE的水分含量、pH值、总可滴定酸度、总可溶性固形物和花青素含量。通过分析淀粉膜的外观、水活度、含水量、厚度、水溶性和水蒸气渗透性来评价含GPE和不含GPE的淀粉膜。GPE具有较高的含水量、酸度和花青素含量。GPE片显示明显的红色，与GPE片相似。随着GPE浓度的增加，膜的厚度(从0.060 mm增加到0.106 mm)、含水量(从8.17增加到12.48%)、水溶性(从13.33增加到33.32%)和水蒸气渗透性(从3.72增加到6.65 g.mm/m2 day kPa)显著增加(p < 0.05)。GPE除了作为增塑剂外，还增加了薄膜的亲水性部分，减少了聚合物链的分子相互作用，并有利于其增溶，这对于可食用薄膜等应用是理想的。葡萄渣掺入竹芋淀粉薄膜是减少葡萄加工副产物的一种很好的替代方法。
{"title":"Development and Characterization of Arrowroot Starch Films Incorporated with Grape Pomace Extract","authors":"Gislaine Ferreira Nogueira, I. B. Soares, C. Soares, F. Fakhouri, R. D. de Oliveira","doi":"10.3390/polysaccharides3010014","DOIUrl":"https://doi.org/10.3390/polysaccharides3010014","url":null,"abstract":"Grape processing residues are a good source of bioactive and nutritional compounds. The incorporation of grape pomace extract (GPE) in starch films can be a strategy for the elaboration of new food packaging products for applications such as edible films or fruit strips. In this context, the objective of this research was to analyze the effect of incorporation and variation of concentration of GPE (0, 20, 30, and 40% mass/mass starch solids) on the physical chemical properties of arrowroot starch edible films created by casting. The GPE was characterized for moisture content, pH, total titratable acidity, total soluble solids, and anthocyanin content. Starch films with and without GPE were evaluated by analyzing their visual appearance, water activity, water content, thickness, water solubility, and water vapor permeability. The GPE had high water content, acidity, and anthocyanins content. The films with GPE showed a noticeable reddish color, similar to observed for the GPE. Increasing the concentration of GPE in the film resulted in significantly increased (p < 0.05) thickness (from 0.060 to 0.106 mm), water content (from 8.17 to 12.48%), solubility in water (from 13.33 to 33.32%), and water vapor permeability (from 3.72 to 6.65 g.mm/m2 day kPa). GPE increased the hydrophilic portion of the film, in addition to acting as a plasticizer, decreasing the molecular interactions of the polymer chain, and favoring its solubilization, which is desirable for applications such as edible films. The elaboration of arrowroot starch films with the incorporation of grape pomace is a good alternative for the reduction of by-products of grape processing.","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76156054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-17DOI: 10.3390/polysaccharides3010013
Eduardo P. Milan, M. Bertolo, V. Martins, S. Bogusz Junior, A. Plepis
In this study, chitosan and collagen (Ch: Col)-based materials containing curcumin (Cur) as a bioactive compound were developed for wound-healing purposes. The effects of incorporating curcumin and increasing its concentration on both the rheological properties of the formed solutions and the morphological and thermal properties of the three-dimensional scaffolds obtained from them were evaluated. Rheology showed that the presence of curcumin resulted in solutions with a solid-like behavior (G’ > G″), higher collagen denaturation temperatures, and higher viscosities, favoring their use as biomaterials for wound healing. A greater cross-linking effect was observed at higher curcumin concentrations, possibly between the amino groups from both polymers and the hydroxyl and keto groups from the polyphenol. Such cross-linking was responsible for the delay in the onset of degradation of the scaffolds by 5 °C, as revealed by thermogravimetric analysis. Moreover, the pore diameter distribution profile of the scaffolds changed with increasing curcumin concentration; a greater number of pores with diameters between 40 and 60 µm was observed for the scaffold with the highest curcumin content (50 mg), which would be the most suitable for the proposed application. Thus, the materials developed in this study are presented as promising biomaterials for their biological evaluation in tissue regeneration.
{"title":"Chitosan and Collagen-Based Materials Enriched with Curcumin (Curcuma longa): Rheological and Morphological Characterization","authors":"Eduardo P. Milan, M. Bertolo, V. Martins, S. Bogusz Junior, A. Plepis","doi":"10.3390/polysaccharides3010013","DOIUrl":"https://doi.org/10.3390/polysaccharides3010013","url":null,"abstract":"In this study, chitosan and collagen (Ch: Col)-based materials containing curcumin (Cur) as a bioactive compound were developed for wound-healing purposes. The effects of incorporating curcumin and increasing its concentration on both the rheological properties of the formed solutions and the morphological and thermal properties of the three-dimensional scaffolds obtained from them were evaluated. Rheology showed that the presence of curcumin resulted in solutions with a solid-like behavior (G’ > G″), higher collagen denaturation temperatures, and higher viscosities, favoring their use as biomaterials for wound healing. A greater cross-linking effect was observed at higher curcumin concentrations, possibly between the amino groups from both polymers and the hydroxyl and keto groups from the polyphenol. Such cross-linking was responsible for the delay in the onset of degradation of the scaffolds by 5 °C, as revealed by thermogravimetric analysis. Moreover, the pore diameter distribution profile of the scaffolds changed with increasing curcumin concentration; a greater number of pores with diameters between 40 and 60 µm was observed for the scaffold with the highest curcumin content (50 mg), which would be the most suitable for the proposed application. Thus, the materials developed in this study are presented as promising biomaterials for their biological evaluation in tissue regeneration.","PeriodicalId":18775,"journal":{"name":"Natural Polysaccharides in Drug Delivery and Biomedical Applications","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76259651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：