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Influence of plant growth-promoting endophytes Colletotrichum siamense and Diaporthe masirevici on tomato plants (Lycopersicon esculentum Mill.). 促进植物生长的内生菌炭疽菌(Colletotrichum siamense)和大孢子菌(Diaporthe masirevici)对番茄植株的影响
IF 4.2 2区 生物学 Q1 MYCOLOGY Pub Date : 2022-03-17 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2050825
Shalene da Silva da Silva Santos, Angela Aparecida da da Silva, Julio Cesar Polonio, Andressa Domingos Polli, Ravely Casarotti Orlandelli, João Arthur Dos Santos Dos Santos Oliveira, José Usan Torres Brandão Filho, João Lúcio Azevedo, João Alencar Pamphile

The protective and growth-promoting activities of Colletrotrichum and Diaporthe endophytes on tomato plants (Lycopersicon esculentum Mill.) are underexplored. We screened 40 endophytic fungi associated with Mexican shrimp plant (Justicia brandegeana) using an in vitro dual culture assay for Fusarium oxysporum, one of the most important phytopathogens of tomato plants. The three best antagonists, Colletotrichum siamense (JB224.g1), C. siamense (JB252.g1), and Diaporthe masirevicii (JB270), were identified based on multilocus sequence analysis. They were assessed in vitro for their inhibition of F. oxysporum and phosphate solubilisation capacity, and for the production of indole acetic acid. Greenhouse experiments verified the growth-promoting effects of these endophytes and the suppression of F. oxysporum symptoms in tomato plants.   Under greenhouse conditions, the JB252.g1 and JB270 isolates showed positive results for seedling emergence speed. The radicular system depth of plants inoculated with JB270 was greater than that in uninoculated plants (27.21 vs 21.95 cm). The soil plant analysis development chlorophyll metre (SPAD) index showed statistically significant results, especially for the endophyte JB224.g1 (36.99) compared to the control plants (30.90) and plants infected solely with F. oxysporum (33.64).

炭疽菌(Colletrotrichum)和Diaporthe内生菌对番茄(lycopersiicon esculentum Mill.)的保护和促生长作用尚未得到充分的研究。采用番茄植物最重要的病原菌之一尖孢镰刀菌(Fusarium oxysporum)的体外双培养试验,筛选了40种与墨西哥对虾(Justicia brandegeana)相关的内生真菌。通过多位点序列分析,确定了3种最佳拮抗剂,分别是炭疽杆菌(Colletotrichum siamense, JB224.g1)、C. siamense (JB252.g1)和麻氏Diaporthe masirevicii (JB270)。在体外评估了它们对尖孢菌的抑制作用和磷酸盐的增溶能力,以及吲哚乙酸的生产能力。温室试验证实了这些内生菌对番茄植株的促生长作用和对尖孢镰刀菌症状的抑制作用。在温室条件下,JB252。g1和JB270分离株的出苗速度呈阳性。JB270接种植株的根系深度大于未接种植株(27.21 cm vs 21.95 cm)。土壤植物分析发育叶绿素计(SPAD)指数具有显著的统计学意义,尤其是内生植物JB224。g1(36.99)与对照植株(30.90)和单纯感染尖孢镰刀菌植株(33.64)比较,差异有统计学意义。
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引用次数: 10
Glyceroglycolipids from the solid culture of Ophiocordyceps sinensis strain LY34 isolated from Tibet of China. 西藏产冬虫夏草LY34菌株固体培养物的甘油糖脂含量。
IF 4.2 2区 生物学 Q1 MYCOLOGY Pub Date : 2022-02-22 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2036841
Baosong Chen, Jinghan Lin, Ao Xu, Dan Yu, Dorji Phurbu, Huanqin Dai, Yi Li, Hongwei Liu

Ophiocordyceps sinensis is a well-known entomogenous fungus with its fruiting bodies or cultural mycelium as food and herbal medicines in Asia. While metabolites which could responsible for its potent pharmaceutical effects has long remained to be elucidated. In this work, chemical investigation on the solid culture of O. sinensis strain LY34 led to the discovery of six digalactosyldiacylglycerols (DGDGS, 1-6) including one new. The structure of compound 1 was determined based on the comprehensive spectra analysis, including NMR, MSn, IR, and chemical derivatisation. Bioactivity studies showed a weak cytotoxicity of compounds 1-6 against 293 T cell and medium anti-inflammatory activity of compounds 1 and 2 on Raw 264.7 cell. The discovery of DGDGs in O. sinensis provides new insight into the pharmacologically active substances.

蛇虫草是一种众所周知的虫生真菌,其子实体或培养菌丝体在亚洲作为食品和草药。而代谢物可能负责其有效的药物作用,长期以来仍有待阐明。本研究通过对O. sinensis菌株LY34固体培养物的化学研究,发现了6种双半乳糖基二酰基甘油(DGDGS, 1-6),其中1种为新化合物。通过NMR、MSn、IR、化学衍生等综合光谱分析确定了化合物1的结构。生物活性研究表明,化合物1-6对293 T细胞具有较弱的细胞毒性,化合物1和2对264.7细胞具有中等的抗炎活性。黄芪中dgdggs的发现为黄芪药理活性物质的研究提供了新的思路。
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引用次数: 0
Optimization for the production of a polyketone 3S,4S-DMD from Panus lecomtei (Agaricomycetes) by submerged fermentation. 琼脂菌深层发酵生产聚酮3S,4S-DMD的优化研究。
IF 4.2 2区 生物学 Q1 MYCOLOGY Pub Date : 2022-02-17 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2036842
Si-Xian Wang, Ping Huang, Hongwei Liu, Yucheng Dai, Xiao-Ling Wang, Gao-Qiang Liu

3,4-Dihydroxy-2,2-dimethyl-chroman derivatives have diverse physiological properties. A polyketone (3S,4S)-3,4-Dihydroxy-6-methoxy-2,2-dimethylchromom (3S,4S-DMD) with antibacterial activity was isolated from the solid culture of rare edible fungus Panus lecomtei. However, the yield of 3S,4S-DMD in solid culture of P. lecomtei is very low and the production period are too long. In this work, efficient accumulation of 3S,4S-DMD in P. lecomtei by submerged fermentation is studied. The key fermentation factors of P. lecomtei for 3S,4S-DMD production were optimised by single-factor experiment successively, and then a Box-Behnken design (BBD) experiment was carried out to further enhance 3S,4S-DMD production. A maximum 3S,4S-DMD yield of 196.3 mg/L was obtained at 25.78 g/L glucose, 1.67 g/L MgSO4 · 7H2O, 40°C and 197 r/min, respectively, which increased by 1.3-fold in comparison with that in the non-optimised fermentation conditions. Furthermore, an enhanced yield of 3S,4S-DMD (261.6 mg/L) was obtained in 5-L agitated fermenter. The 3S,4S-DMD productivity in flask and fermenter reached to 7.26 and 8.07 mg/g per day, respectively, which considerably increased by over 121-fold in comparison with that in the solid fermentation (0.06 mg/g per day). This study presents a potential method for the production of 3S,4S-DMD by submerged fermentation.

3,4-二羟基-2,2-二甲基-铬胺衍生物具有多种生理性质。从稀有食用菌松菇固体培养物中分离到一种具有抗菌活性的聚酮(3S,4S)-3,4-二羟基-6-甲氧基-2,2-二甲基铬(3S,4S- dmd)。然而,在固体培养中,毛草3S、4S-DMD产量很低,生产周期过长。本研究研究了利用深层发酵的方法,高效地积累3S、4S-DMD。通过单因素试验,依次优化了糙皮酵母生产3S、4S-DMD的关键发酵因子,并进行Box-Behnken设计(BBD)试验,进一步提高了3S、4S-DMD的产量。在葡萄糖25.78 g/L、MgSO4·7H2O 1.67 g/L、温度40℃、转速197 r/min条件下,3S、2s - dmd产量最高分别为196.3 mg/L,比未优化的发酵条件提高了1.3倍。此外,在5-L的搅拌发酵罐中,3S,4S-DMD的产量提高了261.6 mg/L。瓶内和发酵罐内3S、4S-DMD产量分别达到7.26和8.07 mg/g / d,较固体发酵(0.06 mg/g / d)显著提高了121倍以上。本研究提出了一种有潜力的深层发酵生产3S、4S-DMD的方法。
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引用次数: 1
Ethnobiological notes and volatile profiles of two rare Chinese desert truffles. 两种稀有中国沙漠松露的民族生物学特征和挥发性特征。
IF 4.2 2区 生物学 Q1 MYCOLOGY Pub Date : 2022-02-14 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2035005
Bin Lu, Feng-Ming Zhang, Fu-Qiang Yu, Andrea C Rinaldi

The production of a distinct profile of volatile organic compounds plays a crucial role in the ecology of hypogeous Ascomycetes, and is also key to their gastronomic relevance. In this study, we explored the aroma components of two rarely investigated Chinese desert truffles, namely Mattirolomyces terfezioides and Choiromyces cerebriformis, using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Our investigation revealed the significant presence of sulphur-containing volatiles in the aroma of M. terfezioides but not in C. cerebriformis. We discussed available information on the distribution of these interesting truffles in China and their use as choice food by local people.

一种独特的挥发性有机化合物的产生在子囊菌的生态学中起着至关重要的作用,也是它们与美食相关的关键。本研究采用顶空固相微萃取(HS-SPME) -气相色谱-质谱联用技术(GC-MS)对两种很少被研究的中国沙漠松露——terfezioides Mattirolomyces和choiroomyces cerebriformis的香气成分进行了研究。我们的研究发现,含硫挥发物在拟特非虫胺的香气中有显著的存在,而在脑虫胺中没有。我们讨论了这些有趣的松露在中国的分布以及它们作为当地人选择的食物的现有信息。
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引用次数: 1
Plants and endophytes - a partnership for the coumarin production through the microbial systems. 植物和内生菌——通过微生物系统生产香豆素的伙伴关系。
IF 4.2 2区 生物学 Q1 MYCOLOGY Pub Date : 2022-02-07 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2027537
Chandrashekar Srinivasa, Govindappa Mellappa, Shashank M Patil, Ramith Ramu, Bhargav Shreevatsa, Chandan Dharmashekar, Shiva Prasad Kollur, Asad Syed, Chandan Shivamallu

Plant-based secondary metabolite production system is well established. However, host-endophyte interaction in the production of secondary metabolite is a new less exploited area that is overcoming barriers and evolving as one of the prospective fields. Endophytes such as bacteria or fungi have the ability to produce some of the secondary metabolites that mimic the plant metabolites therefore escaping the host defence system. Coumarin is one such metabolite with immense biological functions. Most of the studies have demonstrated coumarin production from fungal endophytes but not bacterial endophytes. Herein, we present an overview of all the coumarin derivatives produced from endophytic sources and their biosynthetic pathways. Furthermore, the review also throws light on the isolation of these coumarins and different derivatives with respect to their biological activity. The biotransformation of coumarin derivatives by the action of endophytic fungi is also elaborated. The present review provides an insight on the challenges faced in the coumarin production through fungal endophytes.

以植物为基础的次生代谢物生产体系已经建立。然而,宿主-内生菌相互作用在次生代谢物产生中的作用是一个新的开发较少的领域,正在克服障碍,发展成为一个有前景的领域之一。细菌或真菌等内生菌有能力产生一些模仿植物代谢物的次生代谢物,从而逃脱宿主的防御系统。香豆素就是这样一种具有巨大生物学功能的代谢物。大多数研究表明,香豆素是由真菌内生菌产生的,而不是细菌内生菌。在这里,我们介绍了所有的香豆素衍生物产生的内生来源和他们的生物合成途径的概述。此外,本文还对这些香豆素及其衍生物的分离及其生物活性进行了综述。并对香豆素衍生物在内生真菌作用下的生物转化进行了阐述。本文综述了真菌内生菌生产香豆素所面临的挑战。
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引用次数: 12
Efficient and specific DNA oligonucleotide rRNA probe-based rRNA removal in Talaromyces marneffei 马尔尼菲Talaromyces marneffei中基于rRNA探针的高效特异性DNA寡核苷酸rRNA去除
IF 4.2 2区 生物学 Q1 MYCOLOGY Pub Date : 2022-01-03 DOI: 10.1080/21501203.2021.2017045
Xueyan Hu, Yun Zhang, Minghao Du, E. Yang
ABSTRACT Emerging evidence showed that lncRNAs play important roles in a wide range of biological processes of fungi such as Saccharomyces cerevisiae. However, systemic identification of lncRNAs in non-model fungi is a challenging task as the efficiency of rRNA removal has been proved to be affected by mismatches of universal rRNA-targeting probes of commercial kits, which forces deeper sequencing depth and increases costs. Here, we developed a low-cost and simple rRNA depletion method (rProbe) that could efficiently remove more than 99% rRNA in both yeast and mycelium samples of Talaromyces marneffei. The efficiency and robustness of rProbe were demonstrated to outperform the Illumina Ribo-Zero kit. Using rProbe RNA-seq, we identified 115 differentially expressed lncRNAs and constructed lncRNA-mRNA co-expression network related to dimorphic switch of T. marneffei. Our rRNA removal method has the potential to be a useful tool to explore non-coding transcriptomes of non-model fungi by adjusting rRNA probe sequences species specifically.
越来越多的证据表明,lncrna在酿酒酵母等真菌的广泛生物学过程中发挥着重要作用。然而,非模式真菌中lncrna的系统鉴定是一项具有挑战性的任务,因为rRNA去除的效率已被证明受到商业试剂盒中通用rRNA靶向探针的不匹配的影响,这迫使更深的测序深度并增加了成本。本研究开发了一种低成本、简单的rRNA去除方法(rProbe),该方法可以有效去除马尔尼菲Talaromyces marneffei酵母和菌丝体样品中99%以上的rRNA。rProbe的效率和鲁棒性优于Illumina Ribo-Zero试剂盒。利用rProbe RNA-seq技术,我们鉴定出115个差异表达的lncrna,并构建了与T. marneffei二态开关相关的lncRNA-mRNA共表达网络。我们的rRNA去除方法有可能成为一种有用的工具,通过特异性调节rRNA探针序列来探索非模式真菌的非编码转录组。
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引用次数: 0
Rock-inhabiting fungi: terminology, diversity, evolution and adaptation mechanisms. 岩石真菌:术语、多样性、进化和适应机制。
IF 4.2 2区 生物学 Q1 MYCOLOGY Pub Date : 2021-12-27 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2021.2002452
Bingjie Liu, Rong Fu, Bing Wu, Xingzhong Liu, Meichun Xiang

Rock-inhabiting fungi (RIF) constitute an ecological group associated with terrestrial rocks. This association is generally restricted to the persistent colonisation of rocks and peculiar morphological features based on melanisation and slow growth, which endow RIF with significance in eukaryotic biology, special status in ecology, and exotic potential in biotechnology. There is a need to achieve a better understanding of the hidden biodiversity, antistress biology, origin and convergent evolution of RIF, which will facilitate cultural relic preservation, exploitation of the biogeochemical cycle of rock elements and biotechnology applications. This review focuses on summarising the current knowledge of rock-inhabiting fungi, with particular reference to terminology, biodiversity and geographic distribution, origin and evolution, and stress adaptation mechanisms. We especially teased out the definition through summing up the terms related to rock-inhabting fungi, and also provided a checklist of rock-inhabiting fungal taxa recorded following updated classification schemes.

岩栖真菌(RIF)是一类与陆相岩石相关的生态类群。这种联系通常仅限于岩石的持续定植和基于黑化和缓慢生长的特殊形态特征,这赋予RIF在真核生物学中的重要意义,在生态学中的特殊地位以及在生物技术中的奇异潜力。进一步认识RIF的隐伏生物多样性、抗应激生物学、起源和趋同演化,将有助于文物保护、岩石元素生物地球化学循环的开发和生物技术的应用。本文综述了岩石真菌的相关知识,包括术语、生物多样性和地理分布、起源和进化以及逆境适应机制。我们特别通过总结岩石真菌的相关术语梳理了岩石真菌的定义,并提供了根据更新的分类方案记录的岩石真菌分类清单。
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引用次数: 18
A review of Cystoderma (Agaricales/Basidiomycota) from China with four new species and two new records. 文章题目中国囊皮菌(孢子囊门/担子菌门)4新种2新记录综述。
IF 4.2 2区 生物学 Q1 MYCOLOGY Pub Date : 2021-12-22 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2021.2013969
Li Jia-Xin, He Mao-Qiang, Zhao Rui-Lin

Cystoderma comprises the species with heavily universal veil remnants on basidiomes, weakly to strongly amyloid basidiospores, evanescent floccose-scaly ring zone or persistent membranous ring, which were often encountered in forests and grassland. However, they were less studied than other mushroom groups mainly because of its unclearly phylogenetic position. In this study, we gathered 16 specimens from Southwest and Northwest China, where were the richest biodiversity areas in China, and produced their ITS and nrLSU sequences. The related morphological examinations and molecular phylogenetic analysis showed they belonged to eight species, of which four were new species, and named as Cystoderma lilaceum, C. pseudoamianthinum, C. rugosolateritium, C. subglobisporum, and of which two were new records from China, and they were C. granosum, C. subvinaceum. New species and new records were described in details and discussed with other species. This study not only showed the novel geographical distributions as well as high species diversity of Cystoderma in China, but also provided more research data for the further studies in mushrooms systematics.

囊皮病包括在担子孢子上有大量普遍的面纱残余物,弱至强淀粉样的担子孢子,易逝的絮状鳞片环带或持久的膜状环,在森林和草原中经常遇到。然而,由于其系统发育位置不明确,对其研究较少。本研究从中国生物多样性最丰富的西南和西北地区采集了16个标本,建立了其ITS和nrLSU序列。形态学检查和分子系统发育分析表明,它们分属8种,其中4种为新种,命名为丁香囊皮(Cystoderma lilaceum)、假氨基酸囊皮(C. pseudoamianthinum)、葡萄囊皮(C. rugosolateritium)和亚globisporum,其中2种为中国新记录,分别为C. granosum和C. subvinaceum。对新种和新记录进行了详细描述,并与其他种进行了讨论。本研究不仅显示了囊皮菌在中国新颖的地理分布和丰富的物种多样性,而且为蘑菇系统学的进一步研究提供了更多的研究数据。
{"title":"A review of <i>Cystoderma</i> (Agaricales/Basidiomycota) from China with four new species and two new records.","authors":"Li Jia-Xin,&nbsp;He Mao-Qiang,&nbsp;Zhao Rui-Lin","doi":"10.1080/21501203.2021.2013969","DOIUrl":"https://doi.org/10.1080/21501203.2021.2013969","url":null,"abstract":"<p><p><i>Cystoderma</i> comprises the species with heavily universal veil remnants on basidiomes, weakly to strongly amyloid basidiospores, evanescent floccose-scaly ring zone or persistent membranous ring, which were often encountered in forests and grassland. However, they were less studied than other mushroom groups mainly because of its unclearly phylogenetic position. In this study, we gathered 16 specimens from Southwest and Northwest China, where were the richest biodiversity areas in China, and produced their ITS and nrLSU sequences. The related morphological examinations and molecular phylogenetic analysis showed they belonged to eight species, of which four were new species, and named as <i>Cystoderma lilaceum, C. pseudoamianthinum, C. rugosolateritium, C. subglobisporum</i>, and of which two were new records from China, and they were <i>C. granosum, C. subvinaceum</i>. New species and new records were described in details and discussed with other species. This study not only showed the novel geographical distributions as well as high species diversity of <i>Cystoderma</i> in China, but also provided more research data for the further studies in mushrooms systematics.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 3","pages":"163-176"},"PeriodicalIF":4.2,"publicationDate":"2021-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40689132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Invasive infections due to Magnusiomyces capitatus: case report and review of its prevalence in China. 资本化Magnusiomyces capitatus侵袭性感染:中国病例报告及流行情况综述。
IF 4.2 2区 生物学 Q1 MYCOLOGY Pub Date : 2021-11-22 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2021.2000059
Mingxun Zhu, Liang Yan, Sybren de Hoog, Wanqing Liao, Hong Zhang, Rongfen Zhao, Shuwen Deng

Magnusiomyces capitatus is an emerging opportunistic yeast, thus far mainly reported from the Western world where fungemia is the most frequent presentation in immunocompromised patients with high mortality. We described a rare case of Magnusiomyces capitatus infection from our hospital in China and reviewed six further cases published to date in Chinese literature. It is noted that half more of the cases (4/7) presented with fungemia in younger, immunosuppressed patients, whereas the remaining cases were with pneumonia in elderly, immunocompetent patients. All seven Chinese cases had favourable outcome with antifungal therapy. Based on the limited in vitro and clinical data, a combination of amphotericin B either with 5-fluorocytosine or voriconazole for fungemia in immunocompromised patients, and although fluconazole is not recommended as first-line therapy in the guideline, in our study, fluconazole alone or with 5-fluorocytosine for local pulmonary infection in immunocompetent patients is effective with good outcome.

Magnusiomyces capitatus是一种新兴的机会性酵母,迄今为止主要来自西方世界,在那里真菌血症是免疫功能低下患者中最常见的表现,死亡率高。我们报道了一例罕见的在中国医院发生的头状Magnusiomyces感染病例,并回顾了迄今为止在中国文献中发表的另外6例病例。值得注意的是,一半以上的病例(4/7)在免疫抑制的年轻患者中表现为真菌血症,而其余病例在免疫功能正常的老年患者中表现为肺炎。7例中国病例均经抗真菌治疗后预后良好。基于有限的体外和临床数据,两性霉素B联合5-氟胞嘧啶或伏立康唑治疗免疫功能低下患者的真菌血症,虽然氟康唑在指南中不推荐作为一线治疗,但在我们的研究中,氟康唑单独或联合5-氟胞嘧啶治疗免疫功能正常患者的局部肺部感染是有效的,并且效果良好。
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引用次数: 2
A case of Candida auris candidemia in Xiamen, China, and a comparative analysis of clinical isolates in China. 厦门一例耳念珠菌念珠菌血症病例及中国临床分离株的比较分析。
IF 4.2 2区 生物学 Q1 MYCOLOGY Pub Date : 2021-10-31 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2021.1994479
Jian Bing, Sijia Wang, Heping Xu, Shuru Fan, Han Du, Clarissa J Nobile, Guanghua Huang

The recently emerged fungal pathogen Candida auris often displays resistance to one or more antifungal drugs. Its infections have been identified in at least 40 countries on six continents to date. Here we report a case of C. auris candidemia in a patient in Xiamen, a city in south China. We also review currently reported cases of C. auris infection in China and compare the genetic and biological features of C. auris strains isolated from this country. Our phylogenetic analysis indicates that there are at least two C. auris genetic clades present in China (the South African clade and the south Asian clade) that display opposite mating type loci (one is MTL a and the other is MTLα). We also found that there are several distinct features among the clinical isolates studied, including the expression of virulence factors, antifungal susceptibilities, and cellular morphologies, and that these features could be associated with the mating-type of the isolate. For example, C. auris MTL a isolates generally secreted higher levels of secreted aspartyl proteases (Saps) at ambient environmental temperatures. Taken together, this study demonstrates that C. auris clinical isolates from China exhibit diversity in both biological and genetic features.

最近出现的真菌病原体耳念珠菌通常对一种或多种抗真菌药物表现出耐药性。迄今为止,六大洲至少有40个国家发现了其感染病例。这里我们报告一个C。中国南方城市厦门一名患者的念珠菌血症。我们还审查了目前报告的C。中国金黄色葡萄球菌感染情况的比较。从这个国家分离出的金属菌株。我们的系统发育分析表明,至少有两个C。中国的金属遗传分支(南非分支和南亚分支)显示出相反的交配型基因座(一个是MTL a,另一个是MTLα)。我们还发现,在所研究的临床分离株中有几个不同的特征,包括毒力因子的表达、抗真菌易感性和细胞形态,这些特征可能与分离株的交配类型有关。例如C。auris MTL a分离株通常在环境温度下分泌更高水平的分泌型天冬氨酰蛋白酶(Saps)。总之,这项研究表明,来自中国的金黄色葡萄球菌临床分离株在生物学和遗传学特征上都表现出多样性。
{"title":"A case of <i>Candida auris</i> candidemia in Xiamen, China, and a comparative analysis of clinical isolates in China.","authors":"Jian Bing,&nbsp;Sijia Wang,&nbsp;Heping Xu,&nbsp;Shuru Fan,&nbsp;Han Du,&nbsp;Clarissa J Nobile,&nbsp;Guanghua Huang","doi":"10.1080/21501203.2021.1994479","DOIUrl":"10.1080/21501203.2021.1994479","url":null,"abstract":"<p><p>The recently emerged fungal pathogen <i>Candida auris</i> often displays resistance to one or more antifungal drugs. Its infections have been identified in at least 40 countries on six continents to date. Here we report a case of <i>C. auris</i> candidemia in a patient in Xiamen, a city in south China. We also review currently reported cases of <i>C. auris </i>infection in China and compare the genetic and biological features of <i>C. auris </i>strains isolated from this country. Our phylogenetic analysis indicates that there are at least two <i>C. auris </i>genetic clades present in China (the South African clade and the south Asian clade) that display opposite mating type loci (one is <i>MTL</i> <b>a</b> and the other is <i>MTL</i>α). We also found that there are several distinct features among the clinical isolates studied, including the expression of virulence factors, antifungal susceptibilities, and cellular morphologies, and that these features could be associated with the mating-type of the isolate. For example, <i>C. auris</i> <i>MTL</i> <b>a</b> isolates generally secreted higher levels of secreted aspartyl proteases (Saps) at ambient environmental temperatures. Taken together, this study demonstrates that <i>C. auris</i> clinical isolates from China exhibit diversity in both biological and genetic features.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"13 1","pages":"68-75"},"PeriodicalIF":4.2,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8856026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39939498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
期刊
Mycology
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