Pub Date : 2019-01-01DOI: 10.4172/2329-6836.1000353
Zelalem Ga, Dula De
The bioactive phytochemical constituents present in the plant play a significant role in the development of medicines and drug discovery. Rumex abyssinicus is one of the plants that grow in Ethiopia and different parts of this plant are used as a traditional medicine. The aim of this research was isolation and characterization of bioactive compounds from Rumex abyssinicus rootbark. The plant material was collected from Oromia region around Ilu Ababor Zone, Metu College of teachers’ education campus during February 2016. The dried and powdered plant material was subjected to sequential solvent extraction using maceration technique to prepare the crude extract which was directly used for antibacterial studies. The lists of solvents taken for the extraction were n-hexane, chloroform, acetone and methanol. Antibacterial evaluation of crude extracts of the plant rootbark were screened using in vitro method against four pathogenic bacteria species namely Gram-positive Staphylococcus aureus, Gram negative Escherichia coli, Pseudomonas aeruginosa and Salmonella thyphimurium. These sequentially obtained solvent extracts were filtered and concentrated under reduced pressure. The results showed the acetone extract followed by methanol crude extracts showed inhibitory effects against all of the tested bacterial strains, but the isolated compounds showed inhibitory effects on S. aureus and P. aeruginosa bacterial strains. The acetone extract was subjected to column chromatographic separation that led to isolation of two compounds. The structures of these compounds were characterized with the help of spectroscopic methods (IR and NMR). The isolated compounds were characterized as diisobutyl phthalate (RA-2) (isolated for the first time from the plant) and Emodin (RA-3) based on spectroscopic data and in comparison, with literature reports.
{"title":"Isolation, Characterization and Antibacterial Activities Evaluation of Rumex abyssinicus Rootbark Extracts","authors":"Zelalem Ga, Dula De","doi":"10.4172/2329-6836.1000353","DOIUrl":"https://doi.org/10.4172/2329-6836.1000353","url":null,"abstract":"The bioactive phytochemical constituents present in the plant play a significant role in the development of medicines and drug discovery. Rumex abyssinicus is one of the plants that grow in Ethiopia and different parts of this plant are used as a traditional medicine. The aim of this research was isolation and characterization of bioactive compounds from Rumex abyssinicus rootbark. The plant material was collected from Oromia region around Ilu Ababor Zone, Metu College of teachers’ education campus during February 2016. The dried and powdered plant material was subjected to sequential solvent extraction using maceration technique to prepare the crude extract which was directly used for antibacterial studies. The lists of solvents taken for the extraction were n-hexane, chloroform, acetone and methanol. Antibacterial evaluation of crude extracts of the plant rootbark were screened using in vitro method against four pathogenic bacteria species namely Gram-positive Staphylococcus aureus, Gram negative Escherichia coli, Pseudomonas aeruginosa and Salmonella thyphimurium. These sequentially obtained solvent extracts were filtered and concentrated under reduced pressure. The results showed the acetone extract followed by methanol crude extracts showed inhibitory effects against all of the tested bacterial strains, but the isolated compounds showed inhibitory effects on S. aureus and P. aeruginosa bacterial strains. The acetone extract was subjected to column chromatographic separation that led to isolation of two compounds. The structures of these compounds were characterized with the help of spectroscopic methods (IR and NMR). The isolated compounds were characterized as diisobutyl phthalate (RA-2) (isolated for the first time from the plant) and Emodin (RA-3) based on spectroscopic data and in comparison, with literature reports.","PeriodicalId":18897,"journal":{"name":"Natural products chemistry & research","volume":"39 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83625621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.4172/2329-6836.1000357
S. Arasaretnam, K. Venujah
The molecule of soap consists of two dissimilar ends, a hydrophilic end (polar head) which binds with water and another end which is hydrophobic end (non-polar hydrocarbon tail) that binds with oil. The soap is made by the saponification process, which reacts with the oil that contains triglycerides and lye (NaOH). Oils with different properties make them distinct from each other as the composition of fatty acids is incompatible. In the present study in the process of preparation of soaps, dissimilar oils of 5 types i.e., coconut oil, palm oil, castor oil, olive oil and gee oil were utilized. In order to prepare various soap samples, the oils were blended in different ratios which are then checked to analyze the soap’s quality. In this study amount of volatile matter and moisture content, total fatty mater content, alkali content and pH were determined. The obtained results were compared with some of commercially available soaps such as baby soap (BS-1), elder soap 1 (ES-1), elder soap 2 (ES-2), elder soap 3 (ES-3) and elder soap 4 (ES-4). With the observed studies, the soap made using olive oil was found to have better properties that the others. It has the good alkaline content, TFM value and pH values..
{"title":"Preparation of Soaps by using Different Oil and Analyze their Properties","authors":"S. Arasaretnam, K. Venujah","doi":"10.4172/2329-6836.1000357","DOIUrl":"https://doi.org/10.4172/2329-6836.1000357","url":null,"abstract":"The molecule of soap consists of two dissimilar ends, a hydrophilic end (polar head) which binds with water and another end which is hydrophobic end (non-polar hydrocarbon tail) that binds with oil. The soap is made by the saponification process, which reacts with the oil that contains triglycerides and lye (NaOH). Oils with different properties make them distinct from each other as the composition of fatty acids is incompatible. In the present study in the process of preparation of soaps, dissimilar oils of 5 types i.e., coconut oil, palm oil, castor oil, olive oil and gee oil were utilized. In order to prepare various soap samples, the oils were blended in different ratios which are then checked to analyze the soap’s quality. In this study amount of volatile matter and moisture content, total fatty mater content, alkali content and pH were determined. The obtained results were compared with some of commercially available soaps such as baby soap (BS-1), elder soap 1 (ES-1), elder soap 2 (ES-2), elder soap 3 (ES-3) and elder soap 4 (ES-4). With the observed studies, the soap made using olive oil was found to have better properties that the others. It has the good alkaline content, TFM value and pH values..","PeriodicalId":18897,"journal":{"name":"Natural products chemistry & research","volume":"19 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80719024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.35248/2329-6836.19.7.358
J. Kingsley
{"title":"New Discovery of a Psychoactive/Medicinal Plant (The Oba Plant)","authors":"J. Kingsley","doi":"10.35248/2329-6836.19.7.358","DOIUrl":"https://doi.org/10.35248/2329-6836.19.7.358","url":null,"abstract":"","PeriodicalId":18897,"journal":{"name":"Natural products chemistry & research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86371378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.35248/2329-6836.19.7.364
U. Myadagbadam, Menghini Gd, Y. Wang, S. hasi, Chimedragchaa Ch
To establish a method for the determination of muscone in natural musk by pre-column derivatization HPLC with 2, 4-Dinitrophenyl hydrazine (DNPH) and the reaction conditions were optimized. The influencing factors of derivatization reaction were investigated by single factor, and the main influencing factors were optimized by orthogonal test. Under the optimum reaction conditions, muscone-DNPH derivatives were obtained and detected by 365 nm following the Alltima C18 column (5 μm, 150 mm × 4.6 mm) separation. Single factor investigation revealed: the suitable reaction medium and catalyst were ethanol and hydrochloric acid, respectively; the molar ratio of muscone to DNPH should not be less than 300 times, the appropriate reaction time was 20 min or more; the effect of reaction temperature on derivatization reaction was obvious and needs to be optimized. Furthermore, the orthogonal test of acid concentration, reaction time and temperature showed that the effect of temperature on reaction was significant (p 0.05); The optimum reaction conditions were: reaction temperature was 65°C, hydrochloric acid be added to derivatization solution was (1.25:98.75) (mL/mL) and the reaction time was 30 min. Under this conditions, there was good linearity in range of 0.04 μg•mL-1-30.00 μg•mL-1 for muscone (r=0.999 9), the average spiked recoveries were 98.37%-100.32% and their RSD were 0.57%-1.32%. All the RSD of repeatability of sample and standard treatment were less than 4.12%; the limits of detection (S/N=3) and quantitation (S/N=15) were 0.005 μg•mL-1 and 0.04 μg•mL-1 respectively. The established method for detecting is accurate with good reproducibility and can be used for determination of muscone in natural musk.
{"title":"HPLC Method for Determination and Optimization of 2,4-DNPH PreColumn Derivatization Effect of Reaction Conditions for Muscone in Natural Musk","authors":"U. Myadagbadam, Menghini Gd, Y. Wang, S. hasi, Chimedragchaa Ch","doi":"10.35248/2329-6836.19.7.364","DOIUrl":"https://doi.org/10.35248/2329-6836.19.7.364","url":null,"abstract":"To establish a method for the determination of muscone in natural musk by pre-column derivatization HPLC with 2, 4-Dinitrophenyl hydrazine (DNPH) and the reaction conditions were optimized. The influencing factors of derivatization reaction were investigated by single factor, and the main influencing factors were optimized by orthogonal test. Under the optimum reaction conditions, muscone-DNPH derivatives were obtained and detected by 365 nm following the Alltima C18 column (5 μm, 150 mm × 4.6 mm) separation. Single factor investigation revealed: the suitable reaction medium and catalyst were ethanol and hydrochloric acid, respectively; the molar ratio of muscone to DNPH should not be less than 300 times, the appropriate reaction time was 20 min or more; the effect of reaction temperature on derivatization reaction was obvious and needs to be optimized. Furthermore, the orthogonal test of acid concentration, reaction time and temperature showed that the effect of temperature on reaction was significant (p 0.05); The optimum reaction conditions were: reaction temperature was 65°C, hydrochloric acid be added to derivatization solution was (1.25:98.75) (mL/mL) and the reaction time was 30 min. Under this conditions, there was good linearity in range of 0.04 μg•mL-1-30.00 μg•mL-1 for muscone (r=0.999 9), the average spiked recoveries were 98.37%-100.32% and their RSD were 0.57%-1.32%. All the RSD of repeatability of sample and standard treatment were less than 4.12%; the limits of detection (S/N=3) and quantitation (S/N=15) were 0.005 μg•mL-1 and 0.04 μg•mL-1 respectively. The established method for detecting is accurate with good reproducibility and can be used for determination of muscone in natural musk.","PeriodicalId":18897,"journal":{"name":"Natural products chemistry & research","volume":"57 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76942656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.4172/2329-6836.1000355
P. Milind, Amansowomhan Imuse Jude
Marketed brands of honey in our country do not have much more standardization. Even pharmacopeia standards of honey far from complete. The present study assessed parameters to determine appropriate identification and quality of three brands honey sold in India market. For this purpose, honey of three popular brands of Indian market was selected for evaluation purpose and investigated for following parameters such as macroscopic evaluation, ash value, loss on drying, chemical tests and viscosity. In result study based on macroscopic parameters all three brands were found complies with Indian Pharmacopeia 1996. But in physical evaluation sample of brand D1 was found complies pharmacopeia standards and other two brands S1 and B1 fail in this evaluation even not about near to official minimum standards. On the basis of chemical tests evaluations, we found all three brands complies the official standards as shown in results. As per our overall observations brand D1 found good and most effective of different types of parameters of evaluations.
{"title":"Comparison of Physicochemical Evaluation of Different Brands of Honey","authors":"P. Milind, Amansowomhan Imuse Jude","doi":"10.4172/2329-6836.1000355","DOIUrl":"https://doi.org/10.4172/2329-6836.1000355","url":null,"abstract":"Marketed brands of honey in our country do not have much more standardization. Even pharmacopeia standards of honey far from complete. The present study assessed parameters to determine appropriate identification and quality of three brands honey sold in India market. For this purpose, honey of three popular brands of Indian market was selected for evaluation purpose and investigated for following parameters such as macroscopic evaluation, ash value, loss on drying, chemical tests and viscosity. In result study based on macroscopic parameters all three brands were found complies with Indian Pharmacopeia 1996. But in physical evaluation sample of brand D1 was found complies pharmacopeia standards and other two brands S1 and B1 fail in this evaluation even not about near to official minimum standards. On the basis of chemical tests evaluations, we found all three brands complies the official standards as shown in results. As per our overall observations brand D1 found good and most effective of different types of parameters of evaluations.","PeriodicalId":18897,"journal":{"name":"Natural products chemistry & research","volume":"4 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82499681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-09DOI: 10.20944/preprints201808.0192.v1
Nurul Ain Syukriyah Ahmad Muhamud, S. Amran
Epidemiological studies have proven the influences of tea consumption in prevention of chronic diseases. Tea (Camellia sinensis) is a major source of epigallocatechin-3-gallate (EGCG) compound with pharmacological properties such as anti-obesity, anti-diabetes, anti-inflammation. The aims of this study is to analyze and compare the EGCG content in commercialized tea-based products, infusion tea and ready-to-drink tea beverages available in the local market. This study is divided into two phases. The first phase involved the extraction of EGCG compound from four types of tea namely as white tea (WT), green tea (GT), oolong tea (OT) and black tea (BT) using aqueous and methanol extraction techniques. The amount of EGCG is determined using HPLC. The second phase involved the characterization of EGCG compound in infusion tea bags (ITB) and ready-to-drink tea beverages (RTD) using HPLC. Our findings recorded that GT contain the highest EGCG concentration in comparison to other tea types using both methanol and aqueous extraction methods. Temperature and extraction time have influence on the extraction yield. This study also revealed that ready-to-drink tea beverages contains significantly lower level of EGCG compared to the infusion tea hence makes ready-to-drink beverages less healthy for consumption especially for obese and diabetics individuals.
{"title":"Screening of Epigallocatechin-3-Gallate (EGCG) Content in Camellia Sinensis Products","authors":"Nurul Ain Syukriyah Ahmad Muhamud, S. Amran","doi":"10.20944/preprints201808.0192.v1","DOIUrl":"https://doi.org/10.20944/preprints201808.0192.v1","url":null,"abstract":"Epidemiological studies have proven the influences of tea consumption in prevention of chronic diseases. Tea (Camellia sinensis) is a major source of epigallocatechin-3-gallate (EGCG) compound with pharmacological properties such as anti-obesity, anti-diabetes, anti-inflammation. The aims of this study is to analyze and compare the EGCG content in commercialized tea-based products, infusion tea and ready-to-drink tea beverages available in the local market. This study is divided into two phases. The first phase involved the extraction of EGCG compound from four types of tea namely as white tea (WT), green tea (GT), oolong tea (OT) and black tea (BT) using aqueous and methanol extraction techniques. The amount of EGCG is determined using HPLC. The second phase involved the characterization of EGCG compound in infusion tea bags (ITB) and ready-to-drink tea beverages (RTD) using HPLC. Our findings recorded that GT contain the highest EGCG concentration in comparison to other tea types using both methanol and aqueous extraction methods. Temperature and extraction time have influence on the extraction yield. This study also revealed that ready-to-drink tea beverages contains significantly lower level of EGCG compared to the infusion tea hence makes ready-to-drink beverages less healthy for consumption especially for obese and diabetics individuals.","PeriodicalId":18897,"journal":{"name":"Natural products chemistry & research","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79801637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-04DOI: 10.4172/2329-6836.1000335
Gayatri Chakraborty, K. Manna, B. Debnath, W. Singh, Sanchari Goswami, D. Maiti
In this study, the determination of secondary metabolites, anti-oxidant and cytotoxic activity of seed coat of Macrotyloma uniflorum were carried out in different solvents (petroleum ether, chloroform, ethanol, and water), according to their increasing polarity. Alkaloid and tannin content was analyzed by simple titrimetric method. Colorimetric method was used for the determination of phenolic, flavonoid, terpenoid and saponin content. Antioxidant activity was determined by DPPH free radical scavenging assay and cytotoxic activity was evaluated by Trypan blue exclusion assay. The results indicated that sample seed coat extracted with ethanol (sample 3) contained high level of alkaloid (0.66 ± 0.01 mg/g), phenol (42.38 ± 0.53 mg/g), flavonoid (22.81 ± 0.48 mg/g), tannin (55.89 ± 0.75 mg/g), saponin (24.85 ± 0.53 mg/g), and terpenoid (54.60 ± 1.89 mg/g) and had anti-oxidant and cytotoxic activity against B16F10 and B16BL6 cell line. Further, the results of preliminary DPPH free radical scavenging assay suggested anti-oxidant and Trypan blue exclusion assay on B16F10 and B16BL6 cell line suggested that potent anticancer activity of sample 3 is accompanied.
{"title":"Phytochemical Analysis, Anti-oxidant and Cytotoxic Activity of Seed Coat of Macrotyloma uniflorum in Different Solvents","authors":"Gayatri Chakraborty, K. Manna, B. Debnath, W. Singh, Sanchari Goswami, D. Maiti","doi":"10.4172/2329-6836.1000335","DOIUrl":"https://doi.org/10.4172/2329-6836.1000335","url":null,"abstract":"In this study, the determination of secondary metabolites, anti-oxidant and cytotoxic activity of seed coat of Macrotyloma uniflorum were carried out in different solvents (petroleum ether, chloroform, ethanol, and water), according to their increasing polarity. Alkaloid and tannin content was analyzed by simple titrimetric method. Colorimetric method was used for the determination of phenolic, flavonoid, terpenoid and saponin content. Antioxidant activity was determined by DPPH free radical scavenging assay and cytotoxic activity was evaluated by Trypan blue exclusion assay. The results indicated that sample seed coat extracted with ethanol (sample 3) contained high level of alkaloid (0.66 ± 0.01 mg/g), phenol (42.38 ± 0.53 mg/g), flavonoid (22.81 ± 0.48 mg/g), tannin (55.89 ± 0.75 mg/g), saponin (24.85 ± 0.53 mg/g), and terpenoid (54.60 ± 1.89 mg/g) and had anti-oxidant and cytotoxic activity against B16F10 and B16BL6 cell line. Further, the results of preliminary DPPH free radical scavenging assay suggested anti-oxidant and Trypan blue exclusion assay on B16F10 and B16BL6 cell line suggested that potent anticancer activity of sample 3 is accompanied.","PeriodicalId":18897,"journal":{"name":"Natural products chemistry & research","volume":"33 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2018-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78486770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-23DOI: 10.4172/2329-6836.1000320
A. Roy, Neeru Thakran, N. Bharadvaja
Plumbago zeylanica is a pharmaceutically important plant which belongs to Plumbaginaceae family. It possesses wide range of pharmacological activities which includes antibacterial central nervous system stimulatory activity, anti-fungal, anti-inflammatory, anti-hyperglycemic, anti-cancer and anti-atherosclerotic activity. Fatty acid methyl ester analysis reveals the presence of various fatty acids in the plant. The present study deals with Fatty acid methyl ester profile of five different accession of Plumbago zeylanica. Results reveal that these accessions are rich in Octadecadienoic acid (8-22%), Octadecatrienoic acid (7-24%) and Pentadecanoic acid (11-22%) fatty acids. Accession number 421418 is contains the highest amount of Pentadecanoic acid and 9,12,15 Octadecatrienoic acid. Accession number 439212 contains highest amount of Hexadecanoic acid. Highest amount of 9,12 Octadecadienoic acid was present in accession number 524441.
{"title":"Fatty Acid Methyl Ester Profile Analysis of In-Vitro Grown Accessions of Plumbago zeylanica","authors":"A. Roy, Neeru Thakran, N. Bharadvaja","doi":"10.4172/2329-6836.1000320","DOIUrl":"https://doi.org/10.4172/2329-6836.1000320","url":null,"abstract":"Plumbago zeylanica is a pharmaceutically important plant which belongs to Plumbaginaceae family. It possesses wide range of pharmacological activities which includes antibacterial central nervous system stimulatory activity, anti-fungal, anti-inflammatory, anti-hyperglycemic, anti-cancer and anti-atherosclerotic activity. Fatty acid methyl ester analysis reveals the presence of various fatty acids in the plant. The present study deals with Fatty acid methyl ester profile of five different accession of Plumbago zeylanica. Results reveal that these accessions are rich in Octadecadienoic acid (8-22%), Octadecatrienoic acid (7-24%) and Pentadecanoic acid (11-22%) fatty acids. Accession number 421418 is contains the highest amount of Pentadecanoic acid and 9,12,15 Octadecatrienoic acid. Accession number 439212 contains highest amount of Hexadecanoic acid. Highest amount of 9,12 Octadecadienoic acid was present in accession number 524441.","PeriodicalId":18897,"journal":{"name":"Natural products chemistry & research","volume":"368 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75085939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-06DOI: 10.4172/2329-6836.1000319
Aman Ishaqat, R. Abu-Dahab, Hana M. Hammad, Malek Al-Zihlif, Ismail F. Abaza, Z. D. Nassar, F. Afifi
Elaeagnus angustifolia L. has a long history of use in ethnopharmacology. Only few studies examined the potential activities of the leaves. Furthermore, the leaves’ chemical composition was not fully investigated. In this study, the chemical composition of E. angustifolia leaves extract was analysed and major compounds were isolated and identified. Extract obtained by maceration was further extracted with solvents differing in their polarity then submitted to open column chromatography, followed by isolation of major compounds. They were analysed using UV-Vis and/or NMR. One terpene (β-sitosterol) and four flavonoids (chrysin-7-glucoside, rutin, luteolin and kaempferol) were isolated and identified. For the biological activities, leaves were extracted using ethanol, ethyl acetate, chloroform and water. Anti-angiogenic activity was studied by rat aortic ring assay. Anti-proliferative activity was studied against MCF-7 and T-47D breast cancer cell lines. Ethyl acetate extract was found cytotoxic against T-47D breast cancer cell line (IC50=23.05 μg/mL). Potent anti-angiogenic activity of ethanol-(IC50=3.039 μg/mL), ethyl acetate- (IC50=6.289 μg/mL) and water-extract (IC50=7.153 μg/mL) was reported for the first time.
{"title":"Phytochemical Analysis and Evaluation of Anti-angiogenic and Antiproliferative Activities of the Leaves of Elaeagnus angustifolia L. Grown in Jordan","authors":"Aman Ishaqat, R. Abu-Dahab, Hana M. Hammad, Malek Al-Zihlif, Ismail F. Abaza, Z. D. Nassar, F. Afifi","doi":"10.4172/2329-6836.1000319","DOIUrl":"https://doi.org/10.4172/2329-6836.1000319","url":null,"abstract":"Elaeagnus angustifolia L. has a long history of use in ethnopharmacology. Only few studies examined the potential activities of the leaves. Furthermore, the leaves’ chemical composition was not fully investigated. In this study, the chemical composition of E. angustifolia leaves extract was analysed and major compounds were isolated and identified. Extract obtained by maceration was further extracted with solvents differing in their polarity then submitted to open column chromatography, followed by isolation of major compounds. They were analysed using UV-Vis and/or NMR. One terpene (β-sitosterol) and four flavonoids (chrysin-7-glucoside, rutin, luteolin and kaempferol) were isolated and identified. For the biological activities, leaves were extracted using ethanol, ethyl acetate, chloroform and water. Anti-angiogenic activity was studied by rat aortic ring assay. Anti-proliferative activity was studied against MCF-7 and T-47D breast cancer cell lines. Ethyl acetate extract was found cytotoxic against T-47D breast cancer cell line (IC50=23.05 μg/mL). Potent anti-angiogenic activity of ethanol-(IC50=3.039 μg/mL), ethyl acetate- (IC50=6.289 μg/mL) and water-extract (IC50=7.153 μg/mL) was reported for the first time.","PeriodicalId":18897,"journal":{"name":"Natural products chemistry & research","volume":"70 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89396055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-13DOI: 10.4172/2329-6836.1000317
P. Hr, Rajappa Jj, B. Karmakar, H. Rajanaika, M. RamachandraNaik, Ramesh Tp, P. Chanda, P. Gusain, B. Hs
Synthesis of quinolines containing selenium and sulfur bioactive ligands and their Fe(III) complexes is described. Invitro antioxidant activity were evaluated against, 1,1-diphenyl-2-picryl-hydrazil (DPPH), hydrogen peroxide, superoxide anion radical, reducing power and hydroxyl radical (·HO). At different concentrations, (20-60 μg/mL) the total antioxidant activity of synthesized ligands showed 71, 75 and 83% and complexes showed 76, 85, 94% of inhibition. Where at the same concentration and experimental condition the standard antioxidant such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and α-tocopherol exhibited 94, 95 and 63% inhibitions on peroxidation of linoleic acid emulsion. In support to antioxidants, DNA cleavage investigation of complexes leads to protective effect on DNA cleavage. Hence, this study confirms ligands and their Fe(III)-chelator as antioxidants as well as protect against DNA cleavage.
{"title":"Synthesis of Fe(III) Complexes as Antioxidants and DNA Cleavage Protectors","authors":"P. Hr, Rajappa Jj, B. Karmakar, H. Rajanaika, M. RamachandraNaik, Ramesh Tp, P. Chanda, P. Gusain, B. Hs","doi":"10.4172/2329-6836.1000317","DOIUrl":"https://doi.org/10.4172/2329-6836.1000317","url":null,"abstract":"Synthesis of quinolines containing selenium and sulfur bioactive ligands and their Fe(III) complexes is described. Invitro antioxidant activity were evaluated against, 1,1-diphenyl-2-picryl-hydrazil (DPPH), hydrogen peroxide, superoxide anion radical, reducing power and hydroxyl radical (·HO). At different concentrations, (20-60 μg/mL) the total antioxidant activity of synthesized ligands showed 71, 75 and 83% and complexes showed 76, 85, 94% of inhibition. Where at the same concentration and experimental condition the standard antioxidant such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and α-tocopherol exhibited 94, 95 and 63% inhibitions on peroxidation of linoleic acid emulsion. In support to antioxidants, DNA cleavage investigation of complexes leads to protective effect on DNA cleavage. Hence, this study confirms ligands and their Fe(III)-chelator as antioxidants as well as protect against DNA cleavage.","PeriodicalId":18897,"journal":{"name":"Natural products chemistry & research","volume":"6 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2018-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82232324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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