Pub Date : 2024-10-31DOI: 10.3390/molecules29215157
Marko Bogomolec, Mladena Glavaš, Irena Škorić
BODIPY compounds are important organic dyes with exceptional spectral and photophysical properties and numerous applications in different scientific fields. Their widespread applications have flourished due to their easy structural modifications, which enable the preparation of different molecular structures with tunable spectral and photophysical properties. To date, researchers have mostly devoted their efforts to modifying BODIPY meso-position or pyrrole rings, whereas the substitution of fluorine atoms remains largely unexplored. However, chemistry of the boron atom is possible, and it enables tuning of the photophysical properties of the dyes, without tackling their spectral properties. Furthermore, modifications of boron affect the solubility and aggregation propensity of the molecules. This review article highlights methods for the preparation of 4-substituted compounds and the most important reactions on the boron of the BODIPY dyes. They were divided into reactions promoted by Lewis acid (AlCl3 or BCl3), or bases such as alkoxides and organometallic reagents. By using these two methodologies, it is possible to cleave B-F bonds and substitute them with B-C, B-N, or B-O bonds from different nucleophiles. A special emphasis in this review is given to still underdeveloped photochemical reactions of the boron atom of BODIPY dyes. These reactions have the potential to be used in the development of a new line of BODIPY photo-cleavable protective groups (also known as photocages) with bio-medicinal and photo-pharmacological applications, such as drug delivery.
{"title":"BODIPY Compounds Substituted on Boron.","authors":"Marko Bogomolec, Mladena Glavaš, Irena Škorić","doi":"10.3390/molecules29215157","DOIUrl":"10.3390/molecules29215157","url":null,"abstract":"<p><p>BODIPY compounds are important organic dyes with exceptional spectral and photophysical properties and numerous applications in different scientific fields. Their widespread applications have flourished due to their easy structural modifications, which enable the preparation of different molecular structures with tunable spectral and photophysical properties. To date, researchers have mostly devoted their efforts to modifying BODIPY <i>meso</i>-position or pyrrole rings, whereas the substitution of fluorine atoms remains largely unexplored. However, chemistry of the boron atom is possible, and it enables tuning of the photophysical properties of the dyes, without tackling their spectral properties. Furthermore, modifications of boron affect the solubility and aggregation propensity of the molecules. This review article highlights methods for the preparation of 4-substituted compounds and the most important reactions on the boron of the BODIPY dyes. They were divided into reactions promoted by Lewis acid (AlCl<sub>3</sub> or BCl<sub>3</sub>), or bases such as alkoxides and organometallic reagents. By using these two methodologies, it is possible to cleave B-F bonds and substitute them with B-C, B-N, or B-O bonds from different nucleophiles. A special emphasis in this review is given to still underdeveloped photochemical reactions of the boron atom of BODIPY dyes. These reactions have the potential to be used in the development of a new line of BODIPY photo-cleavable protective groups (also known as photocages) with bio-medicinal and photo-pharmacological applications, such as drug delivery.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"29 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11547857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.3390/molecules29215164
Andrzej Krzykowski, Stanisław Rudy, Renata Polak, Beata Biernacka, Anna Krajewska, Emilia Janiszewska-Turak, Iwona Kowalska, Jerzy Żuchowski, Bartosz Skalski, Dariusz Dziki
Studies were conducted focusing on the drying of chili pepper fruits (Capsicum annuum L.), cultivar Cyklon, using convective (AD), convective-microwave (AMD), vacuum (VD), and freeze-drying (FD) methods. The influence of the drying method and temperature on the kinetics of the process and selected quality attributes of the dried product were evaluated. It was demonstrated that the Midilli model best described the drying kinetics for all methods across the entire measurement range. FD and VD produced dried products with the highest brightness and the greatest value of the a* color parameter. The lowest b* color parameter was observed for the product dried using FD at 40 °C, while the highest b* value was noted for samples dried using AMD (100 W) at 60 °C. The highest carotenoid retention was achieved with the FD method at 40 °C, while the lowest carotenoid content was found in the product obtained using the AMD method (100 W) at 60 °C. The smallest losses of capsaicinoids were observed after FD drying at 40 °C, while the largest were found for AMD (100 W) at 60 °C. The analysis of chili pepper fruit extracts revealed the quantitative composition of 12 main phenolic compounds using the UHPLC-UV method. The highest polyphenol content was obtained with FD, while the lowest total polyphenol content was recorded after AD. Regardless of temperature, the total flavonoid content was highest in extracts from FD products, and the lowest flavonoid content was found after AMD at 100 W. For all drying methods analyzed, the total flavonoid content in the pepper extracts decreased with increasing temperature.
研究重点是使用对流(AD)、对流-微波(AMD)、真空(VD)和冷冻干燥(FD)方法干燥辣椒果实(Capsicum annuum L.)(栽培品种 Cyklon)。评估了干燥方法和温度对过程动力学和干燥产品选定质量属性的影响。结果表明,在整个测量范围内,Midilli 模型最能描述所有方法的干燥动力学。FD 和 VD 生产的干燥产品亮度最高,a* 颜色参数值最大。在 40 °C 下使用 FD 干燥的产品 b* 颜色参数最低,而在 60 °C 下使用 AMD(100 W)干燥的样品 b* 值最高。类胡萝卜素保留率最高的是在 40 °C 下使用 FD 方法获得的产品,而在 60 °C 下使用 AMD 方法(100 瓦)获得的产品中类胡萝卜素含量最低。在 40 °C 下进行 FD 干燥后,辣椒素的损失最小,而在 60 °C 下进行 AMD(100 W)干燥后,辣椒素的损失最大。采用超高效液相色谱-紫外法对辣椒果实提取物进行分析,发现了 12 种主要酚类化合物的定量成分。FD 的多酚含量最高,而 AD 的多酚总含量最低。在所有分析的干燥方法中,辣椒提取物中的总黄酮含量随着温度的升高而降低。
{"title":"Drying of Red Chili Pepper (<i>Capsicum annuum</i> L.): Process Kinetics, Color Changes, Carotenoid Content and Phenolic Profile.","authors":"Andrzej Krzykowski, Stanisław Rudy, Renata Polak, Beata Biernacka, Anna Krajewska, Emilia Janiszewska-Turak, Iwona Kowalska, Jerzy Żuchowski, Bartosz Skalski, Dariusz Dziki","doi":"10.3390/molecules29215164","DOIUrl":"10.3390/molecules29215164","url":null,"abstract":"<p><p>Studies were conducted focusing on the drying of chili pepper fruits (<i>Capsicum annuum</i> L.), cultivar Cyklon, using convective (AD), convective-microwave (AMD), vacuum (VD), and freeze-drying (FD) methods. The influence of the drying method and temperature on the kinetics of the process and selected quality attributes of the dried product were evaluated. It was demonstrated that the Midilli model best described the drying kinetics for all methods across the entire measurement range. FD and VD produced dried products with the highest brightness and the greatest value of the a* color parameter. The lowest b* color parameter was observed for the product dried using FD at 40 °C, while the highest b* value was noted for samples dried using AMD (100 W) at 60 °C. The highest carotenoid retention was achieved with the FD method at 40 °C, while the lowest carotenoid content was found in the product obtained using the AMD method (100 W) at 60 °C. The smallest losses of capsaicinoids were observed after FD drying at 40 °C, while the largest were found for AMD (100 W) at 60 °C. The analysis of chili pepper fruit extracts revealed the quantitative composition of 12 main phenolic compounds using the UHPLC-UV method. The highest polyphenol content was obtained with FD, while the lowest total polyphenol content was recorded after AD. Regardless of temperature, the total flavonoid content was highest in extracts from FD products, and the lowest flavonoid content was found after AMD at 100 W. For all drying methods analyzed, the total flavonoid content in the pepper extracts decreased with increasing temperature.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"29 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11548023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.3390/molecules29215152
Mohd Shoeb, Fouzia Mashkoor, Mohmmad Naved Khan, Changyoon Jeong
Efficiently treating wastewater, particularly the elimination of heavy metal ions from water systems, continues to be one of the most pressing and complex challenges in modern environmental management. In this work, reduced graphene oxide coupled silver molybdate binary nanocomposites (RGO-Ag2MoO4 NCs) have been prepared via hydrothermal method. The crystalline nature and surface properties of the developed RGO-Ag2MoO4 NCs were proved by XRD, FTIR, SEM, and EDS techniques. Adsorption experiments demonstrated that the nanocomposites (NCs) effectively removed Pb(II) ions within 120 min, achieving a maximum removal efficiency ranging from 94.96% to 86.37% for Pb(II) concentrations between 20 and 100 mg/L at pH 6. Kinetic studies showed that the adsorption process followed a pseudo-second order model. Isotherm analysis presented that the Langmuir model provided the greatest fit for the equilibrium data, with a monolayer adsorption capacity of 128.94 mg/g. Thermodynamic analysis revealed that the adsorption process was spontaneous and endothermic. The results of this study highlight RGO-Ag2MoO4 NCs as a highly promising and eco-friendly material for the effective elimination of Pb(II) ions from wastewater. Their strong adsorption capacity, coupled with sustainable properties, makes them an efficient solution for addressing lead contamination, offering significant potential for practical applications in water treatment systems.
{"title":"A Facile Synthesis of RGO-Ag<sub>2</sub>MoO<sub>4</sub> Nanocomposites for Efficient Lead Removal from Aqueous Solution.","authors":"Mohd Shoeb, Fouzia Mashkoor, Mohmmad Naved Khan, Changyoon Jeong","doi":"10.3390/molecules29215152","DOIUrl":"10.3390/molecules29215152","url":null,"abstract":"<p><p>Efficiently treating wastewater, particularly the elimination of heavy metal ions from water systems, continues to be one of the most pressing and complex challenges in modern environmental management. In this work, reduced graphene oxide coupled silver molybdate binary nanocomposites (RGO-Ag<sub>2</sub>MoO<sub>4</sub> NCs) have been prepared via hydrothermal method. The crystalline nature and surface properties of the developed RGO-Ag<sub>2</sub>MoO<sub>4</sub> NCs were proved by XRD, FTIR, SEM, and EDS techniques. Adsorption experiments demonstrated that the nanocomposites (NCs) effectively removed Pb(II) ions within 120 min, achieving a maximum removal efficiency ranging from 94.96% to 86.37% for Pb(II) concentrations between 20 and 100 mg/L at pH 6. Kinetic studies showed that the adsorption process followed a pseudo-second order model. Isotherm analysis presented that the Langmuir model provided the greatest fit for the equilibrium data, with a monolayer adsorption capacity of 128.94 mg/g. Thermodynamic analysis revealed that the adsorption process was spontaneous and endothermic. The results of this study highlight RGO-Ag<sub>2</sub>MoO<sub>4</sub> NCs as a highly promising and eco-friendly material for the effective elimination of Pb(II) ions from wastewater. Their strong adsorption capacity, coupled with sustainable properties, makes them an efficient solution for addressing lead contamination, offering significant potential for practical applications in water treatment systems.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"29 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11547552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.3390/molecules29215174
Qiongjun Zhao, Zixuan Mo, Liuting Zeng, Yue Yuan, Yan Wang, Ying Wang
Hydroxycamptothecin (HCPT) is commonly used in the treatment of liver cancer; however, its low water solubility and poor stability significantly limit its clinical application. In recent years, research on exosomes has deepened considerably. Exosomes possess a unique phospholipid bilayer structure, enabling them to traverse tissue barriers, which provides natural advantages as drug carriers. Nevertheless, delivering exosomes safely and efficiently to target cells remains a major challenge. In this study, we utilized the affinity of the SP94 peptide for human liver cancer cell receptors. HCPT was coated with exosomes in our experimental design, and the exosome membrane was modified with SP94 peptide to facilitate drug delivery to liver cancer cells. Exosomes were purified from bone marrow mesenchymal stem cells, and targeted peptides were attached to their surfaces via post-insertion techniques. Subsequently, HCPT was incorporated into the exosomes through electroporation. Using the HepG2 hepatoma cell line, we evaluated a series of in vitro pharmacodynamics and studied pharmacokinetics and tissue distribution in animal models. The results indicated that ligand-targeted, modified drug-carrying exosomes significantly enhance drug bioavailability, prolong retention time in vivo, and facilitate liver targeting. Moreover, this approach reduces drug nephrotoxicity, enhances anti-tumor efficacy, and lays the groundwork for the development of novel liver cancer-targeting agents.
{"title":"Construction and Evaluation of Hepatic Targeted Drug Delivery System with Hydroxycamptothecin in Stem Cell-Derived Exosomes.","authors":"Qiongjun Zhao, Zixuan Mo, Liuting Zeng, Yue Yuan, Yan Wang, Ying Wang","doi":"10.3390/molecules29215174","DOIUrl":"10.3390/molecules29215174","url":null,"abstract":"<p><p>Hydroxycamptothecin (HCPT) is commonly used in the treatment of liver cancer; however, its low water solubility and poor stability significantly limit its clinical application. In recent years, research on exosomes has deepened considerably. Exosomes possess a unique phospholipid bilayer structure, enabling them to traverse tissue barriers, which provides natural advantages as drug carriers. Nevertheless, delivering exosomes safely and efficiently to target cells remains a major challenge. In this study, we utilized the affinity of the SP94 peptide for human liver cancer cell receptors. HCPT was coated with exosomes in our experimental design, and the exosome membrane was modified with SP94 peptide to facilitate drug delivery to liver cancer cells. Exosomes were purified from bone marrow mesenchymal stem cells, and targeted peptides were attached to their surfaces via post-insertion techniques. Subsequently, HCPT was incorporated into the exosomes through electroporation. Using the HepG2 hepatoma cell line, we evaluated a series of in vitro pharmacodynamics and studied pharmacokinetics and tissue distribution in animal models. The results indicated that ligand-targeted, modified drug-carrying exosomes significantly enhance drug bioavailability, prolong retention time in vivo, and facilitate liver targeting. Moreover, this approach reduces drug nephrotoxicity, enhances anti-tumor efficacy, and lays the groundwork for the development of novel liver cancer-targeting agents.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"29 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11547497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.3390/molecules29215173
Ireneusz Stefaniuk, Bogumił Cieniek, Agata Ćwik, Katarzyna Kluska, Idalia Kasprzyk
Studies on particulate air pollution indicate that a new type of pollutant should be considered from mainly fossil fuel combustion and automobile exhaust emissions, i.e., environmentally persistent free radicals. These radicals, ubiquitous in the environment, have a long life span and are capable of producing harmful reactive oxygen species. Samples of dandelion were collected in 2020 and 2021 in spring and late summer. Roots, leaves, flower stalks, and inflorescences of Taraxacum sp. were collected from six sites with three plants each, along with monitoring of particulate matter air pollution. Four sites were located at streets with heavy traffic and two were control sites in the rural part of the city. The free radical content in each part of the plant was measured by electron paramagnetic resonance. The leaf was selected as the most appropriate part of the plant for the measurement of carbon-derived free radicals. The geff value and the total number of spins were calculated. Relationships were found between location, season, and measurements. The electron paramagnetic resonance spectrum consists of at least two components, which can be attributed to C-type radicals and mixed C + O radicals. Their increase in numbers in the fall seasons, compared to the spring seasons, is also noticeable. It has also been observed that leaves collected in autumn have a higher geff value, which is probably related to the higher amount of oxygen- and carbon-derived free radicals.
对微粒空气污染的研究表明,应考虑一种主要来自化石燃料燃烧和汽车尾气排放的新型污染物,即环境持久性自由基。这些自由基在环境中无处不在,寿命很长,能够产生有害的活性氧。我们在 2020 年和 2021 年的春季和夏末采集了蒲公英样本。在六个地点采集了蒲公英的根、叶、花茎和花序,每个地点采集了三株蒲公英,同时对空气中的颗粒物污染进行了监测。其中四个地点位于交通繁忙的街道,两个地点为城市农村地区的对照地点。通过电子顺磁共振测量了植物各部位的自由基含量。叶片被选为测量碳自由基的最合适部位。计算了 Geff 值和自旋总数。发现了位置、季节和测量之间的关系。电子顺磁共振谱至少由两个部分组成,分别是 C 型自由基和 C + O 混合自由基。与春季相比,它们在秋季的数量明显增加。此外,还观察到秋季采集的树叶具有较高的 geff 值,这可能与氧和碳衍生自由基的数量较多有关。
{"title":"Tracking Long-Lived Free Radicals in Dandelion Caused by Air Pollution Using Electron Paramagnetic Resonance Spectroscopy.","authors":"Ireneusz Stefaniuk, Bogumił Cieniek, Agata Ćwik, Katarzyna Kluska, Idalia Kasprzyk","doi":"10.3390/molecules29215173","DOIUrl":"10.3390/molecules29215173","url":null,"abstract":"<p><p>Studies on particulate air pollution indicate that a new type of pollutant should be considered from mainly fossil fuel combustion and automobile exhaust emissions, i.e., environmentally persistent free radicals. These radicals, ubiquitous in the environment, have a long life span and are capable of producing harmful reactive oxygen species. Samples of dandelion were collected in 2020 and 2021 in spring and late summer. Roots, leaves, flower stalks, and inflorescences of <i>Taraxacum</i> sp. were collected from six sites with three plants each, along with monitoring of particulate matter air pollution. Four sites were located at streets with heavy traffic and two were control sites in the rural part of the city. The free radical content in each part of the plant was measured by electron paramagnetic resonance. The leaf was selected as the most appropriate part of the plant for the measurement of carbon-derived free radicals. The g<sub>eff</sub> value and the total number of spins were calculated. Relationships were found between location, season, and measurements. The electron paramagnetic resonance spectrum consists of at least two components, which can be attributed to C-type radicals and mixed C + O radicals. Their increase in numbers in the fall seasons, compared to the spring seasons, is also noticeable. It has also been observed that leaves collected in autumn have a higher g<sub>eff</sub> value, which is probably related to the higher amount of oxygen- and carbon-derived free radicals.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"29 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11547992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Activating tumor necrosis factor receptor 1 (TNFR1) with tumor necrosis factor alpha (TNFα) is one of the key pathological mechanisms resulting in the exacerbation of rheumatoid arthritis (RA) immune response. Despite various types of drugs being available for the treatment of RA, a series of shortcomings still limits their application. Therefore, developing novel peptide drugs that target TNFα-TNFR1 interaction is expected to expand therapeutic drug options. In this study, the detailed interaction mechanism between TNFα and TNFR1 was elucidated, based on which, a series of linear peptides were initially designed. To overcome its large conformational flexibility, two different head-to-tail cyclization strategies were adopted by adding a proline-glycine (GP) or cysteine-cysteine (CC) to form an amide or disulfide bond between the N-C terminal. The results indicate that two cyclic peptides, R1_CC4 and α_CC8, exhibit the strongest binding free energies. α_CC8 was selected for further optimization using virtual mutations through in vitro activity and toxicity experiments due to its optimal biological activity. The L16R mutant was screened, and its binding affinity to TNFR1 was validated using ELISA assays. This study designed a novel cyclic peptide structure with potential anti-inflammatory properties, possibly bringing an additional choice for the treatment of RA in the future.
{"title":"Discovery of Cyclic Peptide Inhibitors Targeted on TNFα-TNFR1 from Computational Design and Bioactivity Verification.","authors":"Jiangnan Zhang, Huijian Zhao, Qianqian Zhou, Xiaoyue Yang, Haoran Qi, Yongxing Zhao, Longhua Yang","doi":"10.3390/molecules29215147","DOIUrl":"10.3390/molecules29215147","url":null,"abstract":"<p><p>Activating tumor necrosis factor receptor 1 (TNFR1) with tumor necrosis factor alpha (TNFα) is one of the key pathological mechanisms resulting in the exacerbation of rheumatoid arthritis (RA) immune response. Despite various types of drugs being available for the treatment of RA, a series of shortcomings still limits their application. Therefore, developing novel peptide drugs that target TNFα-TNFR1 interaction is expected to expand therapeutic drug options. In this study, the detailed interaction mechanism between TNFα and TNFR1 was elucidated, based on which, a series of linear peptides were initially designed. To overcome its large conformational flexibility, two different head-to-tail cyclization strategies were adopted by adding a proline-glycine (GP) or cysteine-cysteine (CC) to form an amide or disulfide bond between the N-C terminal. The results indicate that two cyclic peptides, R1_CC4 and α_CC8, exhibit the strongest binding free energies. α_CC8 was selected for further optimization using virtual mutations through in vitro activity and toxicity experiments due to its optimal biological activity. The L16R mutant was screened, and its binding affinity to TNFR1 was validated using ELISA assays. This study designed a novel cyclic peptide structure with potential anti-inflammatory properties, possibly bringing an additional choice for the treatment of RA in the future.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"29 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11547827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.3390/molecules29215148
Mirosława Karpińska-Tymoszczyk, Magdalena Surma, Marzena Danowska-Oziewicz, Lidia Kurp, Monika Jabłońska, Karolina Kusek, Tomasz Sawicki
The present study evaluated the physicochemical parameters, polyphenolics content, levels of heat-induced compounds (acrylamide, furfural, 5-hydroxymethylfurfural), antioxidant properties, as well as sensory quality of shortbread cookies enriched with dried sea buckthorn fruit (SBF). The SBF-enriched cookies were prepared by replacing 1, 2, 3, or 5% of the flour with dried sea buckthorn fruit. Our results showed the presence of 12 phenolic acids and 5 flavonoids in the SBF, while two phenolic acids and two flavonoids were detected in the cookies. Most of the compounds were identified in the cookies enriched with 5% SBF. Among the phenolic acids, benzoic acid was the most abundant, while among the flavonoids, quercetin was the most abundant. 5-hydroxymethylofurfural was not detected in any cookies, and the addition of SBF contributed to the presence of furfural in baked products and increased the amount of acrylamide, and their content increased with the amount of SBF addition. The addition of sea buckthorn fruit at 5% distinctly exceeded the benchmark level of acrylamide in the cookies and worsened their sensory quality. It should also be noted that SBF significantly (p < 0.05) improved the antioxidant potential determined by two tests, ABTS and DPPH. The SBF-enriched cookies were characterized by significantly higher values of crispness and browning index compared to the control cookies. The results of the study indicate that SBF can be successfully used as a component of bakery products. In conclusion, the cookies with SBF can show improved technological and functional properties and constitute an added value bakery product that could provide health benefits.
{"title":"The Effects of Enriching Shortbread Cookies with Dried Sea Buckthorn Fruit on the Physicochemical and Sensory Properties.","authors":"Mirosława Karpińska-Tymoszczyk, Magdalena Surma, Marzena Danowska-Oziewicz, Lidia Kurp, Monika Jabłońska, Karolina Kusek, Tomasz Sawicki","doi":"10.3390/molecules29215148","DOIUrl":"10.3390/molecules29215148","url":null,"abstract":"<p><p>The present study evaluated the physicochemical parameters, polyphenolics content, levels of heat-induced compounds (acrylamide, furfural, 5-hydroxymethylfurfural), antioxidant properties, as well as sensory quality of shortbread cookies enriched with dried sea buckthorn fruit (SBF). The SBF-enriched cookies were prepared by replacing 1, 2, 3, or 5% of the flour with dried sea buckthorn fruit. Our results showed the presence of 12 phenolic acids and 5 flavonoids in the SBF, while two phenolic acids and two flavonoids were detected in the cookies. Most of the compounds were identified in the cookies enriched with 5% SBF. Among the phenolic acids, benzoic acid was the most abundant, while among the flavonoids, quercetin was the most abundant. 5-hydroxymethylofurfural was not detected in any cookies, and the addition of SBF contributed to the presence of furfural in baked products and increased the amount of acrylamide, and their content increased with the amount of SBF addition. The addition of sea buckthorn fruit at 5% distinctly exceeded the benchmark level of acrylamide in the cookies and worsened their sensory quality. It should also be noted that SBF significantly (<i>p</i> < 0.05) improved the antioxidant potential determined by two tests, ABTS and DPPH. The SBF-enriched cookies were characterized by significantly higher values of crispness and browning index compared to the control cookies. The results of the study indicate that SBF can be successfully used as a component of bakery products. In conclusion, the cookies with SBF can show improved technological and functional properties and constitute an added value bakery product that could provide health benefits.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"29 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11548027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.3390/molecules29215176
Carmela Spagnuolo, Francesco Mautone, Anna Maria Iole Meola, Stefania Moccia, Giuseppe Di Lorenzo, Carlo Buonerba, Gian Luigi Russo
Bladder cancer, which has a rising incidence, is the 10th most common cancer. The transitional cell carcinoma histotype is aggressive and often current therapies are ineffective. We investigated the anti-proliferative effect of quercetin, a natural flavonoid, in combination with the alkylating agent mafosfamide (MFA) on two human bladder cancer cell lines, namely RT112 and J82, representing the progression from low-grade to high-grade tumors, respectively. In both cell types, the combined treatment led to a synergic reduction in cell viability confirmed by a combination index of less than one, though different biological responses were noted. In J82 cells, MFA alone and, to a lesser extent, with quercetin caused cell cycle arrest in the G2/M phase, but only the combined treatment triggered apoptotic cell death. In contrast, in RT112 cells, quercetin induced autophagy, evidenced by the autophagosome formation and the increase in LC-3 lipidation. Interestingly, the synergistic effect was observed only when cells were pre-treated with MFA for 24 h before adding quercetin, not in the reverse order. This suggests that quercetin may help overcome MFA resistance to apoptosis. Although further studies are needed, investigating the combined effects of quercetin and MFA could help elucidate the mechanisms of drug resistance in bladder cancer treatment.
{"title":"Synergistic Combination of Quercetin and Mafosfamide in Treatment of Bladder Cancer Cells.","authors":"Carmela Spagnuolo, Francesco Mautone, Anna Maria Iole Meola, Stefania Moccia, Giuseppe Di Lorenzo, Carlo Buonerba, Gian Luigi Russo","doi":"10.3390/molecules29215176","DOIUrl":"10.3390/molecules29215176","url":null,"abstract":"<p><p>Bladder cancer, which has a rising incidence, is the 10th most common cancer. The transitional cell carcinoma histotype is aggressive and often current therapies are ineffective. We investigated the anti-proliferative effect of quercetin, a natural flavonoid, in combination with the alkylating agent mafosfamide (MFA) on two human bladder cancer cell lines, namely RT112 and J82, representing the progression from low-grade to high-grade tumors, respectively. In both cell types, the combined treatment led to a synergic reduction in cell viability confirmed by a combination index of less than one, though different biological responses were noted. In J82 cells, MFA alone and, to a lesser extent, with quercetin caused cell cycle arrest in the G2/M phase, but only the combined treatment triggered apoptotic cell death. In contrast, in RT112 cells, quercetin induced autophagy, evidenced by the autophagosome formation and the increase in LC-3 lipidation. Interestingly, the synergistic effect was observed only when cells were pre-treated with MFA for 24 h before adding quercetin, not in the reverse order. This suggests that quercetin may help overcome MFA resistance to apoptosis. Although further studies are needed, investigating the combined effects of quercetin and MFA could help elucidate the mechanisms of drug resistance in bladder cancer treatment.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"29 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11547860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-30DOI: 10.3390/molecules29215137
Martyna Natalia Wieczorek
The coupling of Solid-Phase Microextraction (SPME) technology with gas chromatography (GC) has a well-established and successful history. Traditionally, SPME fibers have been the most popular form thanks to their versatility and the ease with which they can be fully automated. However, alternative geometries for SPME have been developed over the years, beginning with Stir Bar Sorptive Extraction (SBSE) and later evolving into Thin-Film SPME (TF-SPME) devices. Each of these formats offers distinct advantages and disadvantages, which are explored in this study. The primary objective of this study was to comprehensively compare available forms of SPME devices, with a special focus on the advantages of TF-SPME, a novel microextraction method particularly suited for the analysis of odorants in food. The study involved analyzing a standard mixture of 11 key food odorants, representing a range of polarities, to evaluate the efficiency of TF-SPME devices in terms of the number of analytes extracted. Furthermore, four types of TF-SPME devices were compared against each other in both standard mixtures and actual food samples. The final stage of the study employed GCxGC-ToFMS analysis to showcase the potential of the most efficient HLB-TF-SPME device in the non-targeted analysis of complex samples, exemplified by unfiltered wheat beer. This analysis demonstrated the significant capability of HLB-TF-SPME in capturing and identifying a wide range of volatile compounds in complex matrices.
{"title":"Comparison of Different Solid-Phase Microextraction Formats Dedicated to the Analysis of Volatile Compounds-A Comprehensive Study.","authors":"Martyna Natalia Wieczorek","doi":"10.3390/molecules29215137","DOIUrl":"10.3390/molecules29215137","url":null,"abstract":"<p><p>The coupling of Solid-Phase Microextraction (SPME) technology with gas chromatography (GC) has a well-established and successful history. Traditionally, SPME fibers have been the most popular form thanks to their versatility and the ease with which they can be fully automated. However, alternative geometries for SPME have been developed over the years, beginning with Stir Bar Sorptive Extraction (SBSE) and later evolving into Thin-Film SPME (TF-SPME) devices. Each of these formats offers distinct advantages and disadvantages, which are explored in this study. The primary objective of this study was to comprehensively compare available forms of SPME devices, with a special focus on the advantages of TF-SPME, a novel microextraction method particularly suited for the analysis of odorants in food. The study involved analyzing a standard mixture of 11 key food odorants, representing a range of polarities, to evaluate the efficiency of TF-SPME devices in terms of the number of analytes extracted. Furthermore, four types of TF-SPME devices were compared against each other in both standard mixtures and actual food samples. The final stage of the study employed GCxGC-ToFMS analysis to showcase the potential of the most efficient HLB-TF-SPME device in the non-targeted analysis of complex samples, exemplified by unfiltered wheat beer. This analysis demonstrated the significant capability of HLB-TF-SPME in capturing and identifying a wide range of volatile compounds in complex matrices.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"29 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11547884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-30DOI: 10.3390/molecules29215136
Ewa Maria Kratz, Katarzyna Sołkiewicz, Marcin Jędryka
Endometriosis is a gynecological disease for which the diagnostics are difficult and often invasive; therefore, non-invasive diagnostic methods using sensitive and specific parameters present in easily available body fluid such as blood serum are needed for the detection of this disease. Our study aimed to answer the question of whether there are any differences between women with advanced endometriosis (AE), patients with gynecological diseases other than endometriosis (NE), and healthy women (control) in terms of the number of antennas of N-glycans from serum IgG. The degree of branching of IgG N-glycans was determined by a modified lectin ELISA with biotinylated lectin Con A (Canavalia ensiformis agglutinin) recognizing α-linked mannose, specifically reacting with biantennary N-glycans. The PHA-L/Con A ratio was calculated from the obtained N-glycan reactivities with Con A and PHA-L (Phaseolus vulgaris leucoagglutinin, specific to tri- and/or tetra-antennary N-linked glycans). The expression of Con A-reactive biantennary N-glycans in serum IgG was significantly lower in the control group than in the NE group (p = 0.045). The values of the PHA-L/Con A ratio were significantly higher in the NE group than in the AE and control groups (p = 0.019 and p = 0.022, respectively). The PHA-L/Con A ratio could be taken into account as a parameter helpful in the non-invasive diagnosis of advanced endometriosis, thus differentiating this disease from other gynecological diseases with an inflammatory background.
子宫内膜异位症是一种妇科疾病,其诊断比较困难,而且往往是侵入性诊断;因此,需要使用在血清等容易获得的体液中存在的敏感和特异性参数的非侵入性诊断方法来检测这种疾病。我们的研究旨在回答晚期子宫内膜异位症(AE)妇女、子宫内膜异位症以外的妇科疾病患者(NE)和健康妇女(对照组)在血清 IgG 的 N-聚糖天线数量方面是否存在差异的问题。IgG N-聚糖的分支程度是通过改良的凝集素酶联免疫吸附试验测定的,该试验使用生物素化凝集素 Con A(Canavalia ensiformis agglutinin)识别α-连接甘露糖,并与双天线 N-聚糖发生特异性反应。PHA-L/Con A 比值是根据获得的 N-聚糖与 Con A 和 PHA-L(Phaseolus vulgaris leucoagglutinin,特异于三和/或四反式 N-聚糖)的反应性计算得出的。对照组血清 IgG 中 Con A 反应性双ennary N-聚糖的表达量明显低于 NE 组(p = 0.045)。NE 组的 PHA-L/Con A 比值明显高于 AE 组和对照组(分别为 p = 0.019 和 p = 0.022)。PHA-L/Con A 比值可作为一种有助于晚期子宫内膜异位症无创诊断的参数,从而将这种疾病与其他具有炎症背景的妇科疾病区分开来。
{"title":"The Degree of Branching of Serum IgG N-glycans as a Marker of Advanced Endometriosis.","authors":"Ewa Maria Kratz, Katarzyna Sołkiewicz, Marcin Jędryka","doi":"10.3390/molecules29215136","DOIUrl":"10.3390/molecules29215136","url":null,"abstract":"<p><p>Endometriosis is a gynecological disease for which the diagnostics are difficult and often invasive; therefore, non-invasive diagnostic methods using sensitive and specific parameters present in easily available body fluid such as blood serum are needed for the detection of this disease. Our study aimed to answer the question of whether there are any differences between women with advanced endometriosis (AE), patients with gynecological diseases other than endometriosis (NE), and healthy women (control) in terms of the number of antennas of N-glycans from serum IgG. The degree of branching of IgG N-glycans was determined by a modified lectin ELISA with biotinylated lectin Con A (<i>Canavalia ensiformis</i> agglutinin) recognizing α-linked mannose, specifically reacting with biantennary N-glycans. The PHA-L/Con A ratio was calculated from the obtained N-glycan reactivities with Con A and PHA-L (<i>Phaseolus vulgaris</i> leucoagglutinin, specific to tri- and/or tetra-antennary N-linked glycans). The expression of Con A-reactive biantennary N-glycans in serum IgG was significantly lower in the control group than in the NE group (<i>p</i> = 0.045). The values of the PHA-L/Con A ratio were significantly higher in the NE group than in the AE and control groups (<i>p</i> = 0.019 and <i>p</i> = 0.022, respectively). The PHA-L/Con A ratio could be taken into account as a parameter helpful in the non-invasive diagnosis of advanced endometriosis, thus differentiating this disease from other gynecological diseases with an inflammatory background.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"29 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11547903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}