Pub Date : 2026-02-26DOI: 10.1038/s41587-026-03034-2
Hannah L Landecker, Amander T Clark
{"title":"Considerations for the future of in vitro gametogenesis in fertility care.","authors":"Hannah L Landecker, Amander T Clark","doi":"10.1038/s41587-026-03034-2","DOIUrl":"https://doi.org/10.1038/s41587-026-03034-2","url":null,"abstract":"","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":" ","pages":""},"PeriodicalIF":41.7,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147308310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-24DOI: 10.1038/s41587-025-02944-x
Fabian B. H. Rehm, Kim C. Liu, Rongzhen Tian, Jason W. Chin
Organisms that permit hypermutation of target genes without off-target mutagenesis of the host genome enable the accelerated, continuous evolution of genes for new or enhanced functions. We develop and optimize an orthogonal DNA replication system in Escherichiacoli that uses components from bacteriophage Φ29. The minimal system requires just two Φ29 genes to maintain the replicon and replicons can be efficiently engineered in vivo. We generate a highly mutagenic Φ29 DNA polymerase that introduces mutations at a frequency approaching 10 −4 per base per generation (one mutation in a 1-kb gene every ten generations). Our system is stable for hundreds of generations and enables the continuous, accelerated evolution of new gene functions. We demonstrate the rapid evolution of a tetracycline resistance gene to confer resistance to tigecycline at higher levels than achieved with previously reported systems. We further evolve a 1,000-fold increase in β-lactamase activity for a third-generation cephalosporin in just 3 days.
{"title":"Highly mutagenic continuous evolution in E. coli using a Φ29-based orthogonal replication system","authors":"Fabian B. H. Rehm, Kim C. Liu, Rongzhen Tian, Jason W. Chin","doi":"10.1038/s41587-025-02944-x","DOIUrl":"https://doi.org/10.1038/s41587-025-02944-x","url":null,"abstract":"Organisms that permit hypermutation of target genes without off-target mutagenesis of the host genome enable the accelerated, continuous evolution of genes for new or enhanced functions. We develop and optimize an orthogonal DNA replication system in <jats:italic>Escherichia</jats:italic> <jats:italic>coli</jats:italic> that uses components from bacteriophage Φ29. The minimal system requires just two Φ29 genes to maintain the replicon and replicons can be efficiently engineered in vivo. We generate a highly mutagenic Φ29 DNA polymerase that introduces mutations at a frequency approaching 10 <jats:sup>−4</jats:sup> per base per generation (one mutation in a 1-kb gene every ten generations). Our system is stable for hundreds of generations and enables the continuous, accelerated evolution of new gene functions. We demonstrate the rapid evolution of a tetracycline resistance gene to confer resistance to tigecycline at higher levels than achieved with previously reported systems. We further evolve a 1,000-fold increase in β-lactamase activity for a third-generation cephalosporin in just 3 days.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"1 1","pages":""},"PeriodicalIF":46.9,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147279660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-24DOI: 10.1038/s41587-026-03035-1
Binglan Li, Anil Kumar Saini, Jose Guadalupe Hernandez, Jason H. Moore
{"title":"Agentic AI and the rise of in silico team science in biomedical research","authors":"Binglan Li, Anil Kumar Saini, Jose Guadalupe Hernandez, Jason H. Moore","doi":"10.1038/s41587-026-03035-1","DOIUrl":"https://doi.org/10.1038/s41587-026-03035-1","url":null,"abstract":"","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"346 1","pages":""},"PeriodicalIF":46.9,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147279663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-23DOI: 10.1038/s41587-026-03012-8
Iris Marchal
{"title":"Transposable elements in the dark genome.","authors":"Iris Marchal","doi":"10.1038/s41587-026-03012-8","DOIUrl":"https://doi.org/10.1038/s41587-026-03012-8","url":null,"abstract":"","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":" ","pages":""},"PeriodicalIF":41.7,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-19DOI: 10.1038/s41587-026-03006-6
Avital Wagner, Noam Margalit, Yahel Fishman, Shashanka S. Indri, Alexander Upcher, Mark Baranov, Einat Nativ-Roth, Colan E. Hughes, Benson M. Kariuki, Johannes S. Haataja, Lukas Schertel, Jonathan R. Yates, Kenneth D. M. Harris, Allen R. Place, Peter Mojzes, Benjamin A. Palmer
Highly reflective biogenic crystals such as guanine have potential as biocompatible alternatives to toxic inorganic optical materials. However, controlling the structural and optical properties of these sparingly soluble crystals in vitro is challenging. Engineered microbial cells have been used widely to generate high-value metabolites, but the biosynthesis of functional crystalline materials has not been achieved. Here we harness microalgae for the biosynthesis of difficult-to-crystallize molecular materials. We show that dinoflagellates can rapidly accumulate many nitrogen heterocycles from aqueous solutions into nitrogen-storage crystals, revealing a general mechanism for their metabolism of dissolved organic nitrogen. We manipulate this innate crystallization behavior to generate crystals with tailored morphologies and optical properties, including birefringent xanthine spherulites—a biogenic analog of TiO 2 nanoparticles. Our results show how microalgae may be exploited as cellular factories for producing molecular crystals from aqueous solutions, under ambient conditions, harnessing the intrinsic control mechanisms of crystal-forming cells, with possible further applications in the crystallization of pharmaceuticals and bioremediation of toxicants.
{"title":"Harnessing microalgae for the biosynthesis of molecular crystals","authors":"Avital Wagner, Noam Margalit, Yahel Fishman, Shashanka S. Indri, Alexander Upcher, Mark Baranov, Einat Nativ-Roth, Colan E. Hughes, Benson M. Kariuki, Johannes S. Haataja, Lukas Schertel, Jonathan R. Yates, Kenneth D. M. Harris, Allen R. Place, Peter Mojzes, Benjamin A. Palmer","doi":"10.1038/s41587-026-03006-6","DOIUrl":"https://doi.org/10.1038/s41587-026-03006-6","url":null,"abstract":"Highly reflective biogenic crystals such as guanine have potential as biocompatible alternatives to toxic inorganic optical materials. However, controlling the structural and optical properties of these sparingly soluble crystals in vitro is challenging. Engineered microbial cells have been used widely to generate high-value metabolites, but the biosynthesis of functional crystalline materials has not been achieved. Here we harness microalgae for the biosynthesis of difficult-to-crystallize molecular materials. We show that dinoflagellates can rapidly accumulate many nitrogen heterocycles from aqueous solutions into nitrogen-storage crystals, revealing a general mechanism for their metabolism of dissolved organic nitrogen. We manipulate this innate crystallization behavior to generate crystals with tailored morphologies and optical properties, including birefringent xanthine spherulites—a biogenic analog of TiO <jats:sub>2</jats:sub> nanoparticles. Our results show how microalgae may be exploited as cellular factories for producing molecular crystals from aqueous solutions, under ambient conditions, harnessing the intrinsic control mechanisms of crystal-forming cells, with possible further applications in the crystallization of pharmaceuticals and bioremediation of toxicants.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"51 1","pages":""},"PeriodicalIF":46.9,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146223034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-19DOI: 10.1038/s41587-026-03017-3
{"title":"Microalgae as cellular factories for molecular crystals","authors":"","doi":"10.1038/s41587-026-03017-3","DOIUrl":"https://doi.org/10.1038/s41587-026-03017-3","url":null,"abstract":"","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"10 1","pages":""},"PeriodicalIF":46.9,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146223032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-17DOI: 10.1038/s41587-026-03023-5
Iris Marchal
{"title":"TimeVault stores mRNA in cells for later readout","authors":"Iris Marchal","doi":"10.1038/s41587-026-03023-5","DOIUrl":"10.1038/s41587-026-03023-5","url":null,"abstract":"","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"44 2","pages":"193-193"},"PeriodicalIF":41.7,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146206051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-17DOI: 10.1038/s41587-026-03009-3
Recent moves of note in and around the biotech and pharma industries.
生物技术和制药行业的最新动向。
{"title":"People","authors":"","doi":"10.1038/s41587-026-03009-3","DOIUrl":"10.1038/s41587-026-03009-3","url":null,"abstract":"Recent moves of note in and around the biotech and pharma industries.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"44 2","pages":"328-328"},"PeriodicalIF":41.7,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146206062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-17DOI: 10.1038/s41587-026-03019-1
Michael Eisenstein
Computer models are showing the potential to draw upon a person’s clinical records and current health data to forecast diseases and treatment outcomes. Could this presage a new era of preventive medicine?
{"title":"AI tool predicts over 1,000 diseases years before they happen — and more are on the way","authors":"Michael Eisenstein","doi":"10.1038/s41587-026-03019-1","DOIUrl":"10.1038/s41587-026-03019-1","url":null,"abstract":"Computer models are showing the potential to draw upon a person’s clinical records and current health data to forecast diseases and treatment outcomes. Could this presage a new era of preventive medicine?","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"44 2","pages":"163-166"},"PeriodicalIF":41.7,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146206064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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