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RoseTTAFold expands to all-atom for biomolecular prediction and design RoseTTAFold 扩展到全原子生物分子预测和设计
IF 46.9 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-04-17 DOI: 10.1038/s41587-024-02211-5
Iris Marchal
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引用次数: 0
Antivenom slithers back to life 抗蛇毒血清重获新生
IF 46.9 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-04-03 DOI: 10.1038/s41587-024-02221-3
Snakebite treatment still relies on antivenom, a 130-year old technology, but biotech is on the job.
蛇咬伤的治疗仍然依赖抗蛇毒血清,这是一项已有 130 年历史的技术,但生物技术正在发挥作用。
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引用次数: 0
The potential of DAOs for funding and collaborative development in the life sciences DAOs 在生命科学领域的筹资和合作发展潜力
IF 46.9 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-04-02 DOI: 10.1038/s41587-024-02189-0
Simone Fantaccini, Laura Grassi, Andrea Rampoldi
VitaDAO funds longevity research through a blockchain-based decentralized autonomous organization (DAO), showcasing the potential of collaborative, transparent and alternative systems while also highlighting the challenges of coordination, regulation, biases and skepticism in reshaping traditional research financing methods.
VitaDAO 通过基于区块链的去中心化自治组织(DAO)为长寿研究提供资金,展示了协作、透明和替代系统的潜力,同时也凸显了在重塑传统研究融资方法时面临的协调、监管、偏见和怀疑等挑战。
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引用次数: 0
Author Correction: Tumor immunotherapy across MHC barriers using allogeneic T-cell precursors 作者更正:利用异体 T 细胞前体跨越 MHC 障碍的肿瘤免疫疗法。
IF 46.9 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-26 DOI: 10.1038/s41587-024-02205-3
Johannes L. Zakrzewski, David Suh, John C. Markley, Odette M. Smith, Christopher King, Gabrielle L. Goldberg, Robert Jenq, Amanda M. Holland, Jeremy Grubin, Javier Cabrera-Perez, Renier J. Brentjens, Sydney X. Lu, Gabrielle Rizzuto, Derek B. Sant’Angelo, Isabelle Riviere, Michel Sadelain, Glenn Heller, Juan Carlos Zúñiga-Pflücker, Chen Lu, Marcel R. M. van den Brink
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引用次数: 0
Branched chemically modified poly(A) tails enhance the translation capacity of mRNA. 经化学修饰的多聚(A)支链可提高 mRNA 的翻译能力。
IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-22 DOI: 10.1038/s41587-024-02174-7
Hongyu Chen, Dangliang Liu, Jianting Guo, Abhishek Aditham, Yiming Zhou, Jiakun Tian, Shuchen Luo, Jingyi Ren, Alvin Hsu, Jiahao Huang, Franklin Kostas, Mingrui Wu, David R Liu, Xiao Wang

Although messenger RNA (mRNA) has proved effective as a vaccine, its potential as a general therapeutic modality is limited by its instability and low translation capacity. To increase the duration and level of protein expression from mRNA, we designed and synthesized topologically and chemically modified mRNAs with multiple synthetic poly(A) tails. Here we demonstrate that the optimized multitailed mRNA yielded ~4.7-19.5-fold higher luminescence signals than the control mRNA from 24 to 72 h post transfection in cellulo and 14 days detectable signal versus <7 days signal from the control in vivo. We further achieve efficient multiplexed genome editing of the clinically relevant genes Pcsk9 and Angptl3 in mouse liver at a minimal mRNA dosage. Taken together, these results provide a generalizable approach to synthesize capped branched mRNA with markedly enhanced translation capacity.

尽管信使核糖核酸(mRNA)已被证明可以有效地用作疫苗,但由于其不稳定性和较低的翻译能力,其作为一般治疗方法的潜力受到了限制。为了增加 mRNA 蛋白表达的持续时间和水平,我们设计并合成了具有多个合成聚(A)尾的拓扑和化学修饰 mRNA。在这里,我们证明了优化的多尾 mRNA 在细胞转染后 24 到 72 小时内产生的发光信号比对照 mRNA 高出约 4.7-19.5 倍,14 天内可检测到的信号比对照 mRNA 高出约 4.7-19.5 倍。
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引用次数: 0
De novo and somatic structural variant discovery with SVision-pro. 利用 SVision-pro 发现新的和体细胞结构变异。
IF 46.9 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-22 DOI: 10.1038/s41587-024-02190-7
Songbo Wang, Jiadong Lin, Peng Jia, Tun Xu, Xiujuan Li, Yuezhuangnan Liu, Dan Xu, Stephen J Bush, Deyu Meng, Kai Ye

Long-read-based de novo and somatic structural variant (SV) discovery remains challenging, necessitating genomic comparison between samples. We developed SVision-pro, a neural-network-based instance segmentation framework that represents genome-to-genome-level sequencing differences visually and discovers SV comparatively between genomes without any prerequisite for inference models. SVision-pro outperforms state-of-the-art approaches, in particular, the resolving of complex SVs is improved, with low Mendelian error rates, high sensitivity of low-frequency SVs and reduced false-positive rates compared with SV merging approaches.

基于长读数的从头和体细胞结构变异(SV)发现仍然具有挑战性,需要对样本进行基因组比较。我们开发了 SVision-pro,这是一种基于神经网络的实例分割框架,它能直观地表示基因组到基因组水平的测序差异,并在无需任何推理模型的前提下发现基因组间的 SV 比较。与 SV 合并方法相比,SVision-pro 的表现优于最先进的方法,尤其是在解析复杂 SV 方面得到了改进,孟德尔错误率低,对低频 SV 的灵敏度高,假阳性率低。
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引用次数: 0
Making genome editing a success story in Africa 让基因组编辑成为非洲的成功故事
IF 46.9 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-19 DOI: 10.1038/s41587-024-02187-2
Hussein M. Abkallo, Patrick Arbuthnot, Thomas O. Auer, Dave K. Berger, Johan Burger, Ereck Chakauya, Jean-Paul Concordet, Abdoulaye Diabate, Vincenzo Di Donato, Jan-Hendrik Groenewald, Amadou Guindo, Lizette L. Koekemoer, Florence Nazare, Tony Nolan, Fredros Okumu, Emma Orefuwa, Lily Paemka, Lucia Prieto-Godino, Steven Runo, Marie Sadler, Kassahun Tesfaye, Leena Tripathi, Charles Wondji
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引用次数: 0
Biologic patent challenges under the America Invents Act 美国发明法案》下的生物专利挑战
IF 46.9 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-15 DOI: 10.1038/s41587-024-02156-9
Victor L. Van de Wiele, Aaron S. Kesselheim, S. Sean Tu
Administrative patent review procedures are an effective way of correcting erroneously granted biologic patents and may help promote timely drug competition for the benefit of patients and the US healthcare system.
专利行政审查程序是纠正错误授予的生物制剂专利的有效方法,有助于促进及时的药品竞争,造福患者和美国医疗保健系统。
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引用次数: 0
Lighting the match: how a student-led career mentorship program can blaze the way for non-academic careers 点燃火柴:学生主导的职业导师计划如何为非学术职业开辟道路
IF 46.9 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-15 DOI: 10.1038/s41587-024-02170-x
Anna Bagnell, Alana MacDonald, Peter Espenshade, Mark Schenerman
Academic institutions can address the demand for multiskilled individuals who will be competitive in the non-academic job market by establishing a strong career mentorship program.
学术机构可以通过建立强有力的职业指导计划,满足非学术就业市场对具有竞争力的多技能人才的需求。
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引用次数: 0
US bill targets Chinese biotechs 美国针对中国生物技术公司的法案
IF 46.9 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-15 DOI: 10.1038/s41587-024-02191-6
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引用次数: 0
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