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The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells
IF 46.9 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nature biotechnology
Pub Date : 2025-02-17 DOI: 10.1038/s41587-025-02568-1
Chunwei Zheng, Gangming Zhang, Lilia J. Dean, Erik J. Sontheimer, Wen Xue

Reverse transcriptase (RT) has been shown to play a role in double-strand break repair in bacteria, yet the impact of the RT component of prime editors (PEs) on normal mammalian cellular functions is unclear. Here we show that overexpressed RT or PE increases short insertions and diminishes homology-directed repair following Cas9 cleavage at multiple loci in multiple cell lines. Live-cell imaging shows that RT and PEs are rapidly recruited to laser-induced DNA damage sites and promote endogenous repair, independent of known DNA damage sensors. Interestingly, RT–mCherry partially impairs green fluorescent protein–PARP1 recruitment. A compact PE without an RNase H domain shows reduced DNA repair activity and may therefore be more suitable for clinical application. These data reveal a role for untethered RT or the RT domain of PEs in the repair of chromosomal breaks, calling for evaluation of the long-term effect of PEs and retroviral RT in mammalian cells.

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引用次数: 0
Gene and cell therapies for Parkinson’s make headway 帕金森病基因和细胞疗法取得进展
IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nature biotechnology
Pub Date : 2025-02-14 DOI: 10.1038/s41587-025-02581-4
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引用次数: 0
A chromatin-based model for deciphering gene interactions 基于染色质的基因相互作用解密模型
IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nature biotechnology
Pub Date : 2025-02-14 DOI: 10.1038/s41587-025-02573-4
Rong Li
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引用次数: 0
Largest proteome study enlists 14 biopharmas 最大规模的蛋白质组研究涉及 14 家生物制药公司
IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nature biotechnology
Pub Date : 2025-02-14 DOI: 10.1038/s41587-025-02577-0
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引用次数: 0
Colossal raises $200 million to bring back the dodo
IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nature biotechnology
Pub Date : 2025-02-14 DOI: 10.1038/s41587-025-02578-z
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引用次数: 0
Redefining antibody patent protection using paratope mapping and CDR-scanning
IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nature biotechnology
Pub Date : 2025-02-14 DOI: 10.1038/s41587-024-02549-w
Soma S. R. Banik, Xiaoxiang Deng, Edgar Davidson, Ulrich Storz, Benjamin J. Doranz
Patent protection for therapeutic monoclonal antibodies requires a fundamental shift in strategy following Amgen v. Sanofi, which invalidated broad functional claims for antibody genera in the USA.
在安进诉赛诺菲案（Amgen v. Sanofi）之后，治疗性单克隆抗体的专利保护需要从根本上改变策略。
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引用次数: 0
Heat-resistant rice without yield compromise 不影响产量的耐热大米
IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nature biotechnology
Pub Date : 2025-02-14 DOI: 10.1038/s41587-025-02574-3
Alexandra Despang
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引用次数: 0
Startup grows egg proteins in potato fields 初创公司在马铃薯田里种植鸡蛋蛋白
IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nature biotechnology
Pub Date : 2025-02-14 DOI: 10.1038/s41587-025-02563-6
Claire Turrell
{"title":"Startup grows egg proteins in potato fields","authors":"Claire Turrell","doi":"10.1038/s41587-025-02563-6","DOIUrl":"10.1038/s41587-025-02563-6","url":null,"abstract":"","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"43 2","pages":"157-157"},"PeriodicalIF":33.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41587-025-02563-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
De novo-designed protein binders neutralize snake toxins 全新设计的蛋白质结合剂能中和蛇毒素
IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nature biotechnology
Pub Date : 2025-02-14 DOI: 10.1038/s41587-025-02575-2
Anahita Bishop
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引用次数: 0
AI biotechs launch bioprospecting expeditions with Indigenous groups, agree to share benefits 人工智能生物技术公司与土著群体开展生物勘探考察，并同意分享利益
IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nature biotechnology
Pub Date : 2025-02-14 DOI: 10.1038/s41587-025-02564-5
Ben Johnson
Companies are striking unconventional benefit-sharing agreements with national governments to scour planet hotspots, gathering thousands of new DNA and protein sequences to develop into new, commercially useful molecules.
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引用次数: 0
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