首页 > 最新文献

Neuropharmacology最新文献

英文 中文
Effects and mechanisms of anterior thalamus nucleus deep brain stimulation for epilepsy: A scoping review of preclinical studies 丘脑前核深部脑刺激治疗癫痫的效果和机制:临床前研究综述。
IF 4.6 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-08-31 DOI: 10.1016/j.neuropharm.2024.110137
Luciene Covolan , Maria Luiza Motta Pollo , Pedro Bastos dos Santos , Victor Hugo Cardoso Betta , Felipe Farinha Saad Barbosa , Luciano Arnaldo Mian Covolan , Christiane Gimenes , Clement Hamani

Deep brain stimulation (DBS) of the anterior nucleus of the thalamus (ANT) is a safe and effective intervention for the treatment of certain forms of epilepsy. In preclinical models, electrical stimulation of the ANT has antiepileptogenic effects but its underlying mechanisms remain unclear. In this review, we searched multiple databases for studies that described the effects and mechanisms of ANT low or high frequency stimulation (LFS or HFS) in models of epilepsy. Out of 289 articles identified, 83 were pooled for analysis and 34 were included. Overall, ANT DBS was most commonly delivered at high frequency to rodents injected with kainic acid, pilocarpine, or pentylenetetrazole. In most studies, this therapy increased the latency to the first spontaneous seizure and reduced the frequency of seizures by 20%–80%. Electrophysiology data suggested that DBS reduces the severity of electrographic seizures, decreases the duration and increases the threshold of afterdischarges, reduces the power of low-frequency and increase the power high-frequency bands. Mechanistic studies revealed that ANT DBS leads to a series of short- and long-term changes at multiple levels. Some of its anticonvulsant effects were proposed to occur via the modulation of serotonergic and adenosinergic transmission. The latter seems to be derived from the downregulation of adenosine kinase (ADK). ANT DBS was also shown to increase hippocampal levels of lactate, alter the expression of genes involved in calcium signaling, synaptic glutamate, and the NOD-like receptor signaling pathway. When delivered during status epilepticus or following the injection of convulsant agents, DBS was found to reduce the expression of proinflammatory cytokines and apoptosis. When administered chronically, ANT DBS increased the expression of proteins involved in axonal guidance, changed functional connectivity in limbic circuits, and increased the number of hippocampal cells in epileptic animals, suggesting a neuroprotective effect.

对丘脑前核(ANT)进行深部脑刺激(DBS)是治疗某些形式癫痫的一种安全有效的干预措施。在临床前模型中,对丘脑前核的电刺激具有抗致痫作用,但其潜在机制仍不清楚。在这篇综述中,我们检索了多个数据库中描述癫痫模型中 ANT 低频或高频刺激(LFS 或 HFS)效果和机制的研究。在确定的 289 篇文章中,有 83 篇进行了汇总分析,34 篇被纳入其中。总体而言,ANT DBS 最常用于对注射了凯尼酸、皮洛卡品或戊烯四唑的啮齿动物进行高频刺激。在大多数研究中,这种疗法增加了首次自发癫痫发作的潜伏期,并将癫痫发作频率降低了 20%-80% 。电生理学数据表明,DBS 可减轻电图癫痫发作的严重程度、缩短持续时间并提高放电后阈值、降低低频功率并提高高频段功率。机理研究显示,ANT DBS 会导致一系列多层次的短期和长期变化。其中一些抗惊厥作用被认为是通过调节血清素能和腺苷能的传递而产生的。后者似乎来自腺苷激酶(ADK)的下调。ANT DBS 还能增加海马的乳酸水平,改变钙信号、突触谷氨酸和 NOD 样受体信号通路相关基因的表达。在癫痫状态下或注射惊厥剂后给予 DBS,可以减少促炎细胞因子的表达和细胞凋亡。在长期给药的情况下,ANT DBS会增加轴突导向相关蛋白的表达,改变边缘回路的功能连接,并增加癫痫动物海马细胞的数量,这表明它具有神经保护作用。
{"title":"Effects and mechanisms of anterior thalamus nucleus deep brain stimulation for epilepsy: A scoping review of preclinical studies","authors":"Luciene Covolan ,&nbsp;Maria Luiza Motta Pollo ,&nbsp;Pedro Bastos dos Santos ,&nbsp;Victor Hugo Cardoso Betta ,&nbsp;Felipe Farinha Saad Barbosa ,&nbsp;Luciano Arnaldo Mian Covolan ,&nbsp;Christiane Gimenes ,&nbsp;Clement Hamani","doi":"10.1016/j.neuropharm.2024.110137","DOIUrl":"10.1016/j.neuropharm.2024.110137","url":null,"abstract":"<div><p>Deep brain stimulation (DBS) of the anterior nucleus of the thalamus (ANT) is a safe and effective intervention for the treatment of certain forms of epilepsy. In preclinical models, electrical stimulation of the ANT has antiepileptogenic effects but its underlying mechanisms remain unclear. In this review, we searched multiple databases for studies that described the effects and mechanisms of ANT low or high frequency stimulation (LFS or HFS) in models of epilepsy. Out of 289 articles identified, 83 were pooled for analysis and 34 were included. Overall, ANT DBS was most commonly delivered at high frequency to rodents injected with kainic acid, pilocarpine, or pentylenetetrazole. In most studies, this therapy increased the latency to the first spontaneous seizure and reduced the frequency of seizures by 20%–80%. Electrophysiology data suggested that DBS reduces the severity of electrographic seizures, decreases the duration and increases the threshold of afterdischarges, reduces the power of low-frequency and increase the power high-frequency bands. Mechanistic studies revealed that ANT DBS leads to a series of short- and long-term changes at multiple levels. Some of its anticonvulsant effects were proposed to occur via the modulation of serotonergic and adenosinergic transmission. The latter seems to be derived from the downregulation of adenosine kinase (ADK). ANT DBS was also shown to increase hippocampal levels of lactate, alter the expression of genes involved in calcium signaling, synaptic glutamate, and the NOD-like receptor signaling pathway. When delivered during status epilepticus or following the injection of convulsant agents, DBS was found to reduce the expression of proinflammatory cytokines and apoptosis. When administered chronically, ANT DBS increased the expression of proteins involved in axonal guidance, changed functional connectivity in limbic circuits, and increased the number of hippocampal cells in epileptic animals, suggesting a neuroprotective effect.</p></div>","PeriodicalId":19139,"journal":{"name":"Neuropharmacology","volume":"260 ","pages":"Article 110137"},"PeriodicalIF":4.6,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HIV1 gp120 activates microglia via TLR2-nf-κb signaling to up-regulate inflammatory cytokine expression and induce neuropathic pain HIV1 gp120 通过 TLR2-NF-κB 信号激活小胶质细胞,从而上调炎性细胞因子的表达并诱发神经性疼痛。
IF 4.6 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-08-30 DOI: 10.1016/j.neuropharm.2024.110136
Yimeng Sun , Chen Zhang , Tao Lei , Fei Lin , Jian Huang , Yanling Hu , Dan Wang , Wenping Zhang

HIV associated neuropathic pain (HANP) is a common complication of AIDS. Intrathecal injection of recombinant HIV-1 gp120 in mice is a well-known model. Previous RNA sequencing revealed spinal TLR2 acts as a differentially expressed gene in HANP mice. The spinal TLR2 is involved in HANP, but its role and underlying mechanism remains unclear. In this study the transcription, expression and distribution characteristics of TLR2 in the spinal cord of HANP male mice have been analyzed by qRT-PCR, Western blotting, and immunofluorescent staining. We found that TLR2 expression was upregulated in the spinal dorsal horn and mainly distributed in microglial cells, and blocking TLR2 relieved pain of HANP mice. Following stimulation by gp120 microglial cells upregulate TLR2 expression and become activated. The activation stimulates their differentiation into the M1 type, increasing IL-1β and TNF-α expression while inhibiting IL-10 expression. Silencing the Tlr2 gene slows down the activation, polarization, and secretion of pro-inflammatory factors in microglial cells induced by gp120, and enhances the expression of anti-inflammatory factors. Further analysis of the impact of gp120 on downstream signaling pathways of TLR2 in microglial cells, including NF-κB, MAPK (p38MAPK, ERK, and JNK) and PI3K/AKT, revealed that TLR2-NF-κB signaling plays a crucial role in the activation and polarization of microglial cells by gp120. Activation of NF-κB signaling aggravates pain in HANP mice, while blocking it lightens pain. This data indicates that gp120, through the TLR2-NF-κB signaling, activates spinal microglial cells, promotes the secretion of inflammatory cytokines, leading to HANP. This provides new targets to develop drugs for HANP.

艾滋病相关神经病理性疼痛(HANP)是艾滋病的一种常见并发症。小鼠鞘内注射重组 HIV-1 gp120 是一种著名的模型。先前的 RNA 测序发现,脊髓 TLR2 是 HANP 小鼠中的一种差异表达基因。脊髓 TLR2 参与了 HANP,但其作用和内在机制仍不清楚。本研究通过 qRT-PCR、Western 印迹和免疫荧光染色分析了 TLR2 在 HANP 雄性小鼠脊髓中的转录、表达和分布特征。我们发现,TLR2在脊髓背角表达上调,主要分布在小胶质细胞中,阻断TLR2可缓解HANP小鼠的疼痛。在受到 gp120 刺激后,小胶质细胞会上调 TLR2 的表达并被激活。激活会刺激它们分化成 M1 型,增加 IL-1β 和 TNF-α 的表达,同时抑制 IL-10 的表达。沉默 Tlr2 基因会减缓 gp120 诱导的小胶质细胞的激活、极化和促炎因子的分泌,并增强抗炎因子的表达。进一步分析 gp120 对小胶质细胞中 TLR2 的下游信号通路(包括 NF-κB、MAPK(p38MAPK、ERK 和 JNK)和 PI3K/AKT )的影响发现,TLR2-NF-κB 信号在 gp120 对小胶质细胞的激活和极化过程中起着至关重要的作用。激活 NF-κB 信号会加重 HANP 小鼠的疼痛,而阻断 NF-κB 信号则会减轻疼痛。这些数据表明,gp120 通过 TLR2-NF-κB 信号激活脊髓小胶质细胞,促进炎性细胞因子的分泌,从而导致 HANP。这为开发治疗 HANP 的药物提供了新的靶点。
{"title":"HIV1 gp120 activates microglia via TLR2-nf-κb signaling to up-regulate inflammatory cytokine expression and induce neuropathic pain","authors":"Yimeng Sun ,&nbsp;Chen Zhang ,&nbsp;Tao Lei ,&nbsp;Fei Lin ,&nbsp;Jian Huang ,&nbsp;Yanling Hu ,&nbsp;Dan Wang ,&nbsp;Wenping Zhang","doi":"10.1016/j.neuropharm.2024.110136","DOIUrl":"10.1016/j.neuropharm.2024.110136","url":null,"abstract":"<div><p>HIV associated neuropathic pain (HANP) is a common complication of AIDS. Intrathecal injection of recombinant HIV-1 gp120 in mice is a well-known model. Previous RNA sequencing revealed spinal TLR2 acts as a differentially expressed gene in HANP mice. The spinal TLR2 is involved in HANP, but its role and <em>underlying</em> mechanism remains unclear. In this study the transcription, expression and distribution characteristics of TLR2 in the spinal cord of HANP male mice have been analyzed by qRT-PCR, Western blotting, and immunofluorescent staining. We found that TLR2 expression was upregulated in the spinal dorsal horn and mainly distributed in microglial cells, and blocking TLR2 relieved pain of HANP mice. Following stimulation by gp120 microglial cells upregulate TLR2 expression and become activated. The activation stimulates their differentiation into the M1 type, increasing IL-1β and TNF-α expression while inhibiting IL-10 expression. Silencing the <em>Tlr2</em> gene slows down the activation, polarization, and secretion of pro-inflammatory factors in microglial cells induced by gp120, and enhances the expression of anti-inflammatory factors. Further analysis of the impact of gp120 on downstream signaling pathways of TLR2 in microglial cells, including NF-κB, MAPK (p38MAPK, ERK, and JNK) and PI3K/AKT, revealed that TLR2-NF-κB signaling plays a crucial role in the activation and polarization of microglial cells by gp120. Activation of NF-κB signaling aggravates pain in HANP mice, while blocking it lightens pain. This data indicates that gp120, through the TLR2-NF-κB signaling, activates spinal microglial cells, promotes the secretion of inflammatory cytokines, leading to HANP. This provides new targets to develop drugs for HANP.</p></div>","PeriodicalId":19139,"journal":{"name":"Neuropharmacology","volume":"260 ","pages":"Article 110136"},"PeriodicalIF":4.6,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sex differences in the BTBR idiopathic mouse model of autism spectrum disorders: Behavioural and redox-related hippocampal alterations 自闭症谱系障碍 BTBR 特发性小鼠模型的性别差异:行为和氧化还原相关的海马体改变。
IF 4.6 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-08-30 DOI: 10.1016/j.neuropharm.2024.110134
Maria Bove , Vladyslav Sikora , Martina Santoro , Lisa Pia Agosti , Maria Adelaide Palmieri , Stefania Dimonte , Paolo Tucci , Stefania Schiavone , Maria Grazia Morgese , Luigia Trabace

Autism spectrum disorders (ASD) are highly heterogeneous neurodevelopmental diseases. Epidemiological data report that males have been diagnosed with autism more frequently than females. However, recent studies hypothesize that females’ low incidence might be underestimated due to standard clinical measures of ASD behavioural symptoms, mostly derived from males. Indeed, up to now, ASD mouse models focused mainly on males, considering the prevalence of the diagnosis in that sex. Regarding ASD aetiopathogenesis, it has been recently reported that oxidative stress might be implicated in its onset and development, suggesting an association with ASD typical repetitive behaviours that still need to be disentangled. Here, we investigated possible behavioural and molecular sex-related differences by using the BTBR mouse model of idiopathic ASD. To this aim, animals were exposed to behavioural tests related to different ASD core symptoms and comorbidities, i.e. stereotyped repertoire, social dysfunctions, hyperlocomotion and risk-taking behaviours. Moreover, we analyzed hippocampal levels of pro-oxidant and anti-oxidant enzymes, together with biomarkers of oxidative stress and lipid peroxidation.

Our results showed that BTBR females did not display the same patterns for repetitive behaviours as the male counterpart. From a biomolecular point of view, we found an increase in oxidative stress and pro-oxidant enzymes, accompanied by deficient enzymatic anti-oxidant response, only in BTBR males compared to C57BL/6 male mice, while no differences were retrieved in females.

Overall, our study suggests that in females there is an urgent need to depict the distinct ASD symptomatology, accompanied by the identification of sex-specific pharmacological targets.

自闭症谱系障碍(ASD)是一种高度异质性的神经发育疾病。流行病学数据显示,男性比女性更容易被诊断为自闭症。然而,最近的研究假设,女性的低发病率可能被低估了,因为自闭症谱系障碍行为症状的标准临床测量大多来自男性。事实上,到目前为止,考虑到ASD在男性中的发病率,ASD小鼠模型主要以男性为研究对象。关于自闭症的发病机制,最近有报道称氧化应激可能与自闭症的发病和发展有关,这表明氧化应激与自闭症典型的重复行为有关联,但仍需加以区分。在此,我们利用特发性 ASD 的 BTBR 小鼠模型研究了可能存在的行为和分子方面的性别差异。为此,我们对小鼠进行了与不同的ASD核心症状和合并症有关的行为测试,即大理石埋藏、洞板、空地、高架零迷宫和社会交往测试,这些测试包括刻板印象、社会功能障碍、过度运动和冒险行为。此外,我们还分析了海马中促氧化酶和抗氧化酶的水平,以及氧化应激和脂质过氧化的生物标志物。结果表明,BTBR雌鼠的重复行为模式与雄鼠不同。从生物分子的角度来看,我们发现与 C57BL/6 雄性小鼠相比,只有 BTBR 雄性小鼠的氧化应激和促氧化酶增加,同时酶的抗氧化反应不足,而雌性小鼠则没有发现差异。总之,我们的研究表明,迫切需要对雌性小鼠的独特 ASD 症状进行描述,同时确定性别特异性药理靶点。
{"title":"Sex differences in the BTBR idiopathic mouse model of autism spectrum disorders: Behavioural and redox-related hippocampal alterations","authors":"Maria Bove ,&nbsp;Vladyslav Sikora ,&nbsp;Martina Santoro ,&nbsp;Lisa Pia Agosti ,&nbsp;Maria Adelaide Palmieri ,&nbsp;Stefania Dimonte ,&nbsp;Paolo Tucci ,&nbsp;Stefania Schiavone ,&nbsp;Maria Grazia Morgese ,&nbsp;Luigia Trabace","doi":"10.1016/j.neuropharm.2024.110134","DOIUrl":"10.1016/j.neuropharm.2024.110134","url":null,"abstract":"<div><p>Autism spectrum disorders (ASD) are highly heterogeneous neurodevelopmental diseases. Epidemiological data report that males have been diagnosed with autism more frequently than females. However, recent studies hypothesize that females’ low incidence might be underestimated due to standard clinical measures of ASD behavioural symptoms, mostly derived from males. Indeed, up to now, ASD mouse models focused mainly on males, considering the prevalence of the diagnosis in that sex. Regarding ASD aetiopathogenesis, it has been recently reported that oxidative stress might be implicated in its onset and development, suggesting an association with ASD typical repetitive behaviours that still need to be disentangled. Here, we investigated possible behavioural and molecular sex-related differences by using the BTBR mouse model of idiopathic ASD. To this aim, animals were exposed to behavioural tests related to different ASD core symptoms and comorbidities, <em>i.e.</em> stereotyped repertoire, social dysfunctions, hyperlocomotion and risk-taking behaviours. Moreover, we analyzed hippocampal levels of pro-oxidant and anti-oxidant enzymes, together with biomarkers of oxidative stress and lipid peroxidation.</p><p>Our results showed that BTBR females did not display the same patterns for repetitive behaviours as the male counterpart. From a biomolecular point of view, we found an increase in oxidative stress and pro-oxidant enzymes, accompanied by deficient enzymatic anti-oxidant response, only in BTBR males compared to C57BL/6 male mice, while no differences were retrieved in females.</p><p>Overall, our study suggests that in females there is an urgent need to depict the distinct ASD symptomatology, accompanied by the identification of sex-specific pharmacological targets.</p></div>","PeriodicalId":19139,"journal":{"name":"Neuropharmacology","volume":"260 ","pages":"Article 110134"},"PeriodicalIF":4.6,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0028390824003034/pdfft?md5=d86f5a258c6d9560cc8b1b529e40b201&pid=1-s2.0-S0028390824003034-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GPx1-ERK1/2-CREB pathway regulates the distinct vulnerability of hippocampal neurons to oxidative stress via modulating mitochondrial dynamics following status epilepticus 癫痫状态后,GPx1-ERK1/2-CREB通路通过调节线粒体动力学调节海马神经元对氧化应激的独特脆弱性。
IF 4.6 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-08-29 DOI: 10.1016/j.neuropharm.2024.110135
Ji-Eun Kim, Duk-Shin Lee, Su Hyeon Wang, Tae-Hyun Kim, Tae-Cheon Kang

Glutathione peroxidase-1 (GPx1) and cAMP/Ca2+ responsive element (CRE)-binding protein (CREB) regulate neuronal viability by maintaining the redox homeostasis. Since GPx1 and CREB reciprocally regulate each other, it is likely that GPx1-CREB interaction may play a neuroprotective role against oxidative stress, which are largely unknown. Thus, we investigated the underlying mechanisms of the reciprocal regulation between GPx1 and CREB in the male rat hippocampus. Under physiological condition, L-buthionine sulfoximine (BSO)-induced oxidative stress increased GPx1 expression, extracellular signal-regulated kinase 1/2 (ERK1/2) activity and CREB serine (S) 133 phosphorylation in CA1 neurons, but not dentate granule cells (DGC), which were diminished by GPx1 siRNA, U0126 or CREB knockdown. GPx1 knockdown inhibited ERK1/2 and CREB activations induced by BSO. CREB knockdown also decreased the efficacy of BSO on ERK1/2 activation. BSO facilitated dynamin-related protein 1 (DRP1)-mediated mitochondrial fission in CA1 neurons, which abrogated by GPx1 knockdown and U0126. CREB knockdown blunted BSO-induced DRP1 upregulation without affecting DRP1 S616 phosphorylation ratio. Following status epilepticus (SE), GPx1 expression was reduced in CA1 neurons and DGC. SE also decreased CREB activity CA1 neurons, but not DGC. SE degenerated CA1 neurons, but not DGC, accompanied by mitochondrial elongation. These post-SE events were ameliorated by N-acetylcysteine (NAC, an antioxidant), but deteriorated by GPx1 knockdown. These findings indicate that a transient GPx1-ERK1/2-CREB activation may be a defense mechanism to protect hippocampal neurons against oxidative stress via maintenance of proper mitochondrial dynamics.

谷胱甘肽过氧化物酶-1(GPx1)和 cAMP/Ca2+ 反应元件(CRE)结合蛋白(CREB)通过维持氧化还原平衡来调节神经元的活力。由于 GPx1 和 CREB 相互调控,GPx1-CREB 相互作用很可能对氧化应激起到神经保护作用,而这一作用在很大程度上是未知的。因此,我们研究了雄性大鼠海马中 GPx1 和 CREB 相互调控的内在机制。在生理条件下,L-丁硫磺酰亚胺(BSO)诱导的氧化应激会增加CA1神经元中GPx1的表达、细胞外信号调节激酶1/2(ERK1/2)的活性和CREB丝氨酸(S)133磷酸化,但不会增加齿状颗粒细胞(DGC)的表达、ERK1/2活性和CREB丝氨酸(S)133磷酸化。GPx1 敲除抑制了 BSO 诱导的 ERK1/2 和 CREB 激活。CREB 敲除也降低了 BSO 对 ERK1/2 激活的功效。BSO促进了CA1神经元中Dynamin相关蛋白1(DRP1)介导的线粒体裂变,而GPx1基因敲除和U0126则可抑制这种裂变。CREB 敲除可减弱 BSO 诱导的 DRP1 上调,但不会影响 DRP1 S616 磷酸化比率。癫痫状态(SE)后,GPx1在CA1神经元和DGC中的表达减少。SE还降低了CA1神经元的CREB活性,但没有降低DGC的CREB活性。SE使CA1神经元变性,但没有使DGC变性,同时伴有线粒体伸长。N-乙酰半胱氨酸(NAC,一种抗氧化剂)可改善这些 SE 后事件,但 GPx1 基因敲除则会使其恶化。这些发现表明,瞬时 GPx1-ERK1/2-CREB 激活可能是一种防御机制,可通过维持线粒体的正常动态来保护海马神经元免受氧化应激。
{"title":"GPx1-ERK1/2-CREB pathway regulates the distinct vulnerability of hippocampal neurons to oxidative stress via modulating mitochondrial dynamics following status epilepticus","authors":"Ji-Eun Kim,&nbsp;Duk-Shin Lee,&nbsp;Su Hyeon Wang,&nbsp;Tae-Hyun Kim,&nbsp;Tae-Cheon Kang","doi":"10.1016/j.neuropharm.2024.110135","DOIUrl":"10.1016/j.neuropharm.2024.110135","url":null,"abstract":"<div><p>Glutathione peroxidase-1 (GPx1) and cAMP/Ca<sup>2+</sup> responsive element (CRE)-binding protein (CREB) regulate neuronal viability by maintaining the redox homeostasis. Since GPx1 and CREB reciprocally regulate each other, it is likely that GPx1-CREB interaction may play a neuroprotective role against oxidative stress, which are largely unknown. Thus, we investigated the underlying mechanisms of the reciprocal regulation between GPx1 and CREB in the male rat hippocampus. Under physiological condition, L-buthionine sulfoximine (BSO)-induced oxidative stress increased GPx1 expression, extracellular signal-regulated kinase 1/2 (ERK1/2) activity and CREB serine (S) 133 phosphorylation in CA1 neurons, but not dentate granule cells (DGC), which were diminished by GPx1 siRNA, U0126 or CREB knockdown. GPx1 knockdown inhibited ERK1/2 and CREB activations induced by BSO. CREB knockdown also decreased the efficacy of BSO on ERK1/2 activation. BSO facilitated dynamin-related protein 1 (DRP1)-mediated mitochondrial fission in CA1 neurons, which abrogated by GPx1 knockdown and U0126. CREB knockdown blunted BSO-induced DRP1 upregulation without affecting DRP1 S616 phosphorylation ratio. Following status epilepticus (SE), GPx1 expression was reduced in CA1 neurons and DGC. SE also decreased CREB activity CA1 neurons, but not DGC. SE degenerated CA1 neurons, but not DGC, accompanied by mitochondrial elongation. These post-SE events were ameliorated by N-acetylcysteine (NAC, an antioxidant), but deteriorated by GPx1 knockdown. These findings indicate that a transient GPx1-ERK1/2-CREB activation may be a defense mechanism to protect hippocampal neurons against oxidative stress via maintenance of proper mitochondrial dynamics.</p></div>","PeriodicalId":19139,"journal":{"name":"Neuropharmacology","volume":"260 ","pages":"Article 110135"},"PeriodicalIF":4.6,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dopaminergic system and neurons: Role in multiple neurological diseases 多巴胺能系统和神经元:在多种神经系统疾病中的作用
IF 4.6 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-08-27 DOI: 10.1016/j.neuropharm.2024.110133
Heng Chen , Jieshu Li , Zhixing Huang , Xiaoxiao Fan , Xiaofei Wang , Xing Chen , Haitao Guo , Hao Liu , Shuqi Li , Shaojun Yu , Honghong Li , Xinyu Huang , Xuehua Ma , Xinqi Deng , Chunguo Wang , Yonggang Liu

The dopaminergic system is a complex and powerful neurotransmitter system in the brain. It plays an important regulatory role in motivation, reward, cognition, and motor control. In recent decades, research in the field of the dopaminergic system and neurons has increased exponentially and is gradually becoming a point of intervention in the study and understanding of a wide range of neurological diseases related to human health. Studies have shown that the dopaminergic system and neurons are involved in the development of many neurological diseases (including, but not limited to Parkinson's disease, schizophrenia, depression, attention deficit hyperactivity disorder, etc.) and that dopaminergic neurons either have too much stress or too weak function in the dopaminergic system can lead to disease. Therefore, targeting dopaminergic neurons is considered key to treating these diseases. This article provides a comprehensive review of the dopaminergic system and neurons in terms of brain region distribution, physiological function and subtypes of dopaminergic neurons, as well as the role of the dopaminergic system and neurons in a variety of diseases.

多巴胺能系统是大脑中一种复杂而强大的神经递质系统。它在动机、奖赏、认知和运动控制方面发挥着重要的调节作用。近几十年来,多巴胺能系统和神经元领域的研究成倍增长,并逐渐成为研究和了解与人类健康相关的各种神经系统疾病的介入点。研究表明,多巴胺能系统和神经元与多种神经系统疾病(包括但不限于帕金森病、精神分裂症、抑郁症、注意力缺陷多动障碍等)的发生发展有关,多巴胺能神经元压力过大或多巴胺能系统功能过弱都会导致疾病的发生。因此,靶向多巴胺能神经元被认为是治疗这些疾病的关键。本文从多巴胺能神经元的脑区分布、生理功能和亚型,以及多巴胺能系统和神经元在多种疾病中的作用等方面对多巴胺能系统和神经元进行了全面综述。
{"title":"Dopaminergic system and neurons: Role in multiple neurological diseases","authors":"Heng Chen ,&nbsp;Jieshu Li ,&nbsp;Zhixing Huang ,&nbsp;Xiaoxiao Fan ,&nbsp;Xiaofei Wang ,&nbsp;Xing Chen ,&nbsp;Haitao Guo ,&nbsp;Hao Liu ,&nbsp;Shuqi Li ,&nbsp;Shaojun Yu ,&nbsp;Honghong Li ,&nbsp;Xinyu Huang ,&nbsp;Xuehua Ma ,&nbsp;Xinqi Deng ,&nbsp;Chunguo Wang ,&nbsp;Yonggang Liu","doi":"10.1016/j.neuropharm.2024.110133","DOIUrl":"10.1016/j.neuropharm.2024.110133","url":null,"abstract":"<div><p>The dopaminergic system is a complex and powerful neurotransmitter system in the brain. It plays an important regulatory role in motivation, reward, cognition, and motor control. In recent decades, research in the field of the dopaminergic system and neurons has increased exponentially and is gradually becoming a point of intervention in the study and understanding of a wide range of neurological diseases related to human health. Studies have shown that the dopaminergic system and neurons are involved in the development of many neurological diseases (including, but not limited to Parkinson's disease, schizophrenia, depression, attention deficit hyperactivity disorder, etc.) and that dopaminergic neurons either have too much stress or too weak function in the dopaminergic system can lead to disease. Therefore, targeting dopaminergic neurons is considered key to treating these diseases. This article provides a comprehensive review of the dopaminergic system and neurons in terms of brain region distribution, physiological function and subtypes of dopaminergic neurons, as well as the role of the dopaminergic system and neurons in a variety of diseases.</p></div>","PeriodicalId":19139,"journal":{"name":"Neuropharmacology","volume":"260 ","pages":"Article 110133"},"PeriodicalIF":4.6,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The PGC-1α/ERRα/ULK1 pathway contributes to Perioperative neurocognitive disorders by inducing mitochondrial dysfunction and activating NLRP3 inflammasome in aged mice PGC-1α/ERRα/ULK1通路通过诱导线粒体功能障碍和激活NLRP3炎症体导致老年小鼠围手术期神经认知障碍
IF 4.6 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-08-27 DOI: 10.1016/j.neuropharm.2024.110119
Wen Zhang , Cui-cui Wu , Meng-Meng Ge , Xiao-Man Yuan , Si-Yi Han , Feng-Tian Zhao , Xiao-Yu Zhang , Feng Gao , Yu-Ke Tian , Guang-Xiong Zhang , Xue-Bi Tian

Perioperative neurocognitive disorders (PND) are intractable, indistinct, and considerably diminish the postoperative quality of life of patients. It has been proved that Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) was involved in neurodegenerative diseases by regulating mitochondrial biogenesis. The underlying mechanisms of PGC-1α and Nod-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasome in PND are not well understood. In this study, we constructed a model of laparotomy in aged mice, and then examined the cognition changes with novel object recognition tests and fear condition tests. The protein levels of PGC-1α and NLRP3 in the hippocampus were detect after surgery. Our results showed that NLRP3 and downstream PI3K/AKT pathway expressions were augmented in the hippocampus after surgery, whereas, the expressions of PGC-1α/estrogen-related receptor α (ERRα)/Unc-51-like autophagy activating kinase 1 (ULK1) pathway were diminished after surgery. In addition, we found that NLRP3 was mainly co-localized with neurons in the hippocampus, and synaptic-related proteins were reduced after surgery. At the same time, transmission electron microscopy (TEM) showed that mitochondria were impaired after surgery. Pharmacological treatment of MCC950, a selective NLRP3 inhibitor, effectively alleviated PND. Activation of PGC-1α with ZLN005 significantly ameliorated PND by enhancing the PGC-1α/ERRα/ULK1 signaling pathway, and further suppressing NLRP3 activation. As a result, we conclude that suppression of the PGC-1α/ERRα/ULK1 signaling pathway is the primary mechanism of PND which caused mitochondrial dysfunction, and activated NLRP3 inflammasome and downstream PI3K/AKT pathway, eventually improved cognitive dysfunction.

围手术期神经认知障碍(PND)是一种顽固性、不明确的疾病,大大降低了患者的术后生活质量。已有研究证实,过氧化物酶体增殖激活受体-γ辅助激活因子-1α(PGC-1α)通过调节线粒体生物生成参与神经退行性疾病的发生。PGC-1α和含Nod样受体吡啉结构域蛋白3(NLRP3)炎性小体在PND中的作用机制尚不十分清楚。在本研究中,我们构建了老年小鼠开腹手术模型,并通过新物体识别测试和恐惧条件测试检测了小鼠的认知变化。我们检测了手术后海马中PGC-1α和NLRP3的蛋白水平。结果显示,手术后海马中NLRP3及下游PI3K/AKT通路的表达增加,而PGC-1α/雌激素相关受体α(ERRα)/Unc-51样自噬激活激酶1(ULK1)通路的表达减少。此外,我们还发现 NLRP3 主要与海马中的神经元共定位,术后突触相关蛋白减少。同时,透射电子显微镜(TEM)显示术后线粒体受损。选择性NLRP3抑制剂MCC950的药物治疗有效缓解了PND。用 ZLN005 激活 PGC-1α 可增强 PGC-1α/ERRα/ULK1 信号通路,进一步抑制 NLRP3 的激活,从而显著改善 PND。因此,我们认为抑制PGC-1α/ERRα/ULK1信号通路是PND导致线粒体功能障碍、激活NLRP3炎性体和下游PI3K/AKT通路并最终改善认知功能障碍的主要机制。
{"title":"The PGC-1α/ERRα/ULK1 pathway contributes to Perioperative neurocognitive disorders by inducing mitochondrial dysfunction and activating NLRP3 inflammasome in aged mice","authors":"Wen Zhang ,&nbsp;Cui-cui Wu ,&nbsp;Meng-Meng Ge ,&nbsp;Xiao-Man Yuan ,&nbsp;Si-Yi Han ,&nbsp;Feng-Tian Zhao ,&nbsp;Xiao-Yu Zhang ,&nbsp;Feng Gao ,&nbsp;Yu-Ke Tian ,&nbsp;Guang-Xiong Zhang ,&nbsp;Xue-Bi Tian","doi":"10.1016/j.neuropharm.2024.110119","DOIUrl":"10.1016/j.neuropharm.2024.110119","url":null,"abstract":"<div><p>Perioperative neurocognitive disorders (PND) are intractable, indistinct, and considerably diminish the postoperative quality of life of patients. It has been proved that Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) was involved in neurodegenerative diseases by regulating mitochondrial biogenesis. The underlying mechanisms of PGC-1α and Nod-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasome in PND are not well understood. In this study, we constructed a model of laparotomy in aged mice, and then examined the cognition changes with novel object recognition tests and fear condition tests. The protein levels of PGC-1α and NLRP3 in the hippocampus were detect after surgery. Our results showed that NLRP3 and downstream PI3K/AKT pathway expressions were augmented in the hippocampus after surgery, whereas, the expressions of PGC-1α/estrogen-related receptor α (ERRα)/Unc-51-like autophagy activating kinase 1 (ULK1) pathway were diminished after surgery. In addition, we found that NLRP3 was mainly co-localized with neurons in the hippocampus, and synaptic-related proteins were reduced after surgery. At the same time, transmission electron microscopy (TEM) showed that mitochondria were impaired after surgery. Pharmacological treatment of MCC950, a selective NLRP3 inhibitor, effectively alleviated PND. Activation of PGC-1α with ZLN005 significantly ameliorated PND by enhancing the PGC-1α/ERRα/ULK1 signaling pathway, and further suppressing NLRP3 activation. As a result, we conclude that suppression of the PGC-1α/ERRα/ULK1 signaling pathway is the primary mechanism of PND which caused mitochondrial dysfunction, and activated NLRP3 inflammasome and downstream PI3K/AKT pathway, eventually improved cognitive dysfunction.</p></div>","PeriodicalId":19139,"journal":{"name":"Neuropharmacology","volume":"260 ","pages":"Article 110119"},"PeriodicalIF":4.6,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142093573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid reorganization of serotonin projections and antidepressant response to 5-HT1A-biased agonist NLX-101 in fluoxetine-resistant cF1ko mice 氟西汀耐药 cF1ko 小鼠血清素投射的快速重组和对 5-HT1A 偏激动剂 NLX-101 的抗抑郁反应
IF 4.6 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-08-27 DOI: 10.1016/j.neuropharm.2024.110132
Faranak Vahid-Ansari , Adrian Newman-Tancredi , Alberto Francisco Fuentes-Alvarenga , Mireille Daigle , Paul R. Albert

Selective serotonin (5-HT) reuptake inhibitors (SSRIs) like fluoxetine remain a first-line treatment for major depression, but are effective in less than half of patients and can take 4–8 weeks to show results. In this study, we examined cF1ko mice with genetically induced upregulation of 5-HT1A autoreceptors that reduces 5-HT neuronal activity. These mice display anxiety- and depression-related behaviors that did not respond to chronic fluoxetine treatment. We examined treatment with NLX-101, a biased agonist that preferentially targets 5-HT1A heteroreceptors. By testing different doses of NLX-101, we found that a dose of 0.2 mg/kg was effective in reducing depression-related behavior in cF1ko mice without causing hypothermia, a 5-HT1A autoreceptor-mediated response. After 1 h, this dose activated dorsal raphe 5-HT neurons and cells in the medial prefrontal cortex (mPFC), increasing nuclear c-fos labelling in cF1ko mice. In cF1ko mice but not wild-type littermates, 0.2 mg/kg NLX-101 administered 1 h prior to each behavioral test for two weeks reduced depressive behavior in the forced swim test, but increased anxiety-related behaviors in the open field, elevated plus maze, and novelty suppressed feeding tests. During this treatment, NLX-101 induced widespread increases in the density of 5-HT axons, varicosities, and especially synaptic and triadic structures, particularly in depression-related brain regions including mPFC, hippocampal CA1 and CA2/3, amygdala and nucleus accumbens of cF1ko mice. Overall, NLX-101 was rapid and effective in reducing depressive behavior in SSRI-resistant mice, but also induced anxiety-related behaviors. The increase in serotonin innervation induced by intermittent NLX-101 may contribute to its behavioral actions.

氟西汀等选择性血清素(5-HT)再摄取抑制剂(SSRIs)仍是治疗重度抑郁症的一线药物,但对不到一半的患者有效,而且需要 4-8 周才能显效。在这项研究中,我们研究了 cF1ko 小鼠,它们的 5-HT1A 自体受体基因诱导上调,从而降低了 5-HT 神经元的活性。这些小鼠表现出与焦虑和抑郁相关的行为,但对长期氟西汀治疗无反应。我们对 NLX-101 的治疗进行了研究,NLX-101 是一种优先靶向 5-HT1A 异受体的偏向激动剂。通过测试不同剂量的 NLX-101,我们发现 0.2 毫克/千克的剂量能有效减少 cF1ko 小鼠的抑郁相关行为,而不会导致体温过低(一种 5-HT1A 自身受体介导的反应)。一小时后,该剂量激活了背侧剑突 5-HT 神经元和内侧前额叶皮层(mPFC)细胞,增加了 cF1ko 小鼠核 c-fos 标记。对 cF1ko 小鼠而非野生型同窝小鼠,在连续两周的每次行为测试前一小时施用 0.2 毫克/千克 NLX-101 会减少强迫游泳测试中的抑郁行为,但会增加开阔地、高架加迷宫和新奇抑制喂食测试中的焦虑相关行为。在治疗过程中,NLX-101 会诱导 5-HT 轴突、变异体,特别是突触和三联体结构密度的广泛增加,尤其是在抑郁相关脑区,包括 cF1ko 小鼠的 mPFC、海马 CA1 和 CA2/3、杏仁核和伏隔核。总体而言,NLX-101 能快速有效地减少 SSRI 抗性小鼠的抑郁行为,但也会诱发焦虑相关行为。间歇性 NLX-101 引起的血清素神经支配的增加可能是其行为作用的原因之一。
{"title":"Rapid reorganization of serotonin projections and antidepressant response to 5-HT1A-biased agonist NLX-101 in fluoxetine-resistant cF1ko mice","authors":"Faranak Vahid-Ansari ,&nbsp;Adrian Newman-Tancredi ,&nbsp;Alberto Francisco Fuentes-Alvarenga ,&nbsp;Mireille Daigle ,&nbsp;Paul R. Albert","doi":"10.1016/j.neuropharm.2024.110132","DOIUrl":"10.1016/j.neuropharm.2024.110132","url":null,"abstract":"<div><p>Selective serotonin (5-HT) reuptake inhibitors (SSRIs) like fluoxetine remain a first-line treatment for major depression, but are effective in less than half of patients and can take 4–8 weeks to show results. In this study, we examined cF1ko mice with genetically induced upregulation of 5-HT1A autoreceptors that reduces 5-HT neuronal activity. These mice display anxiety- and depression-related behaviors that did not respond to chronic fluoxetine treatment. We examined treatment with NLX-101, a biased agonist that preferentially targets 5-HT1A heteroreceptors. By testing different doses of NLX-101, we found that a dose of 0.2 mg/kg was effective in reducing depression-related behavior in cF1ko mice without causing hypothermia, a 5-HT1A autoreceptor-mediated response. After 1 h, this dose activated dorsal raphe 5-HT neurons and cells in the medial prefrontal cortex (mPFC), increasing nuclear c-fos labelling in cF1ko mice. In cF1ko mice but not wild-type littermates, 0.2 mg/kg NLX-101 administered 1 h prior to each behavioral test for two weeks reduced depressive behavior in the forced swim test, but increased anxiety-related behaviors in the open field, elevated plus maze, and novelty suppressed feeding tests. During this treatment, NLX-101 induced widespread increases in the density of 5-HT axons, varicosities, and especially synaptic and triadic structures, particularly in depression-related brain regions including mPFC, hippocampal CA1 and CA2/3, amygdala and nucleus accumbens of cF1ko mice. Overall, NLX-101 was rapid and effective in reducing depressive behavior in SSRI-resistant mice, but also induced anxiety-related behaviors. The increase in serotonin innervation induced by intermittent NLX-101 may contribute to its behavioral actions.</p></div>","PeriodicalId":19139,"journal":{"name":"Neuropharmacology","volume":"261 ","pages":"Article 110132"},"PeriodicalIF":4.6,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0028390824003010/pdfft?md5=72086ec5144c0e0728aebe4520c29492&pid=1-s2.0-S0028390824003010-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of four-week intranasal oxytocin administration on large-scale brain networks in older adults 连续四周鼻内注射催产素对老年人大规模大脑网络的影响
IF 4.6 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-08-23 DOI: 10.1016/j.neuropharm.2024.110130
Peiwei Liu , Tian Lin , Håkan Fischer , David Feifel , Natalie C. Ebner

Oxytocin (OT) is a crucial modulator of social cognition and behavior. Previous work primarily examined effects of acute intranasal oxytocin administration (IN-OT) in younger males on isolated brain regions. Not well understood are (i) chronic IN-OT effects, (ii) in older adults, (iii) on large-scale brain networks, representative of OT's wider-ranging brain mechanisms. To address these research gaps, 60 generally healthy older adults (mean age = 70.12 years, range = 55–83) were randomly assigned to self-administer either IN-OT or placebo twice daily via nasal spray over four weeks. Chronic IN-OT reduced resting-state functional connectivity (rs-FC) of both the right insula and the left middle cingulate cortex with the salience network but enhanced rs-FC of the left medial prefrontal cortex with the default mode network as well as the left thalamus with the basal ganglia–thalamus network. No significant chronic IN-OT effects were observed for between-network rs-FC. However, chronic IN-OT increased selective rs-FC of the basal ganglia–thalamus network with the salience network and the default mode network, indicative of more specialized, efficient communication between these networks. Directly comparing chronic vs. acute IN-OT, reduced rs-FC of the right insula with the salience network and between the default mode network and the basal ganglia–thalamus network, and greater selective rs-FC of the salience network with the default mode network and the basal ganglia–thalamus network, were more pronounced after chronic than acute IN-OT. Our results delineate the modulatory role of IN-OT on large-scale brain networks among older adults.

催产素(OT)是社会认知和行为的重要调节剂。以往的工作主要研究了年轻男性急性鼻内注射催产素(IN-OT)对孤立脑区的影响。而对于(i)慢性 IN-OT 作用,(ii)对老年人的影响,(iii)对大规模大脑网络的影响(代表催产素更广泛的大脑机制),目前还不甚了解。为了填补这些研究空白,60 位身体健康的老年人(平均年龄为 70.12 岁,年龄范围为 55-83 岁)被随机分配到通过鼻腔喷雾每天两次自行服用 IN-OT 或安慰剂,为期四周。慢性IN-OT降低了右侧岛叶和左侧中扣带回皮层与显著性网络的静息态功能连接(rs-FC),但增强了左侧内侧前额叶皮层与默认模式网络以及左侧丘脑与基底节-丘脑网络的rs-FC。在网络间的rs-FC方面,没有观察到明显的慢性IN-OT效应。但是,慢性 IN-OT 增加了丘脑基底节网络与显著性网络和默认模式网络的选择性 rs-FC,表明这些网络之间的交流更加专业化和高效。直接比较慢性 IN-OT 和急性 IN-OT,发现慢性 IN-OT 后,右侧岛叶与显著性网络以及默认模式网络和基底神经节-丘脑网络之间的选择性 rs-FC 减少,而显著性网络与默认模式网络和基底神经节-丘脑网络之间的选择性 rs-FC 增加。我们的研究结果阐明了 IN-OT 对老年人大规模大脑网络的调节作用。
{"title":"Effects of four-week intranasal oxytocin administration on large-scale brain networks in older adults","authors":"Peiwei Liu ,&nbsp;Tian Lin ,&nbsp;Håkan Fischer ,&nbsp;David Feifel ,&nbsp;Natalie C. Ebner","doi":"10.1016/j.neuropharm.2024.110130","DOIUrl":"10.1016/j.neuropharm.2024.110130","url":null,"abstract":"<div><p>Oxytocin (OT) is a crucial modulator of social cognition and behavior. Previous work primarily examined effects of acute intranasal oxytocin administration (IN-OT) in younger males on isolated brain regions. Not well understood are <em>(i)</em> chronic IN-OT effects, <em>(ii)</em> in older adults, <em>(iii)</em> on large-scale brain networks, representative of OT's wider-ranging brain mechanisms. To address these research gaps, 60 generally healthy older adults (mean age = 70.12 years, range = 55–83) were randomly assigned to self-administer either IN-OT or placebo twice daily via nasal spray over four weeks. Chronic IN-OT reduced resting-state functional connectivity (rs-FC) of both the right insula and the left middle cingulate cortex with the salience network but enhanced rs-FC of the left medial prefrontal cortex with the default mode network as well as the left thalamus with the basal ganglia–thalamus network. No significant chronic IN-OT effects were observed for between-network rs-FC. However, chronic IN-OT increased selective rs-FC of the basal ganglia–thalamus network with the salience network and the default mode network, indicative of more specialized, efficient communication between these networks. Directly comparing chronic vs. acute IN-OT, reduced rs-FC of the right insula with the salience network and between the default mode network and the basal ganglia–thalamus network, and greater selective rs-FC of the salience network with the default mode network and the basal ganglia–thalamus network, were more pronounced after chronic than acute IN-OT. Our results delineate the modulatory role of IN-OT on large-scale brain networks among older adults.</p></div>","PeriodicalId":19139,"journal":{"name":"Neuropharmacology","volume":"260 ","pages":"Article 110130"},"PeriodicalIF":4.6,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MiR-184-3p in the paraventricular nucleus participates in the neurobiology of depression via regulation of the hypothalamus-pituitary-adrenal axis 室旁核中的 MiR-184-3p 通过调节下丘脑-垂体-肾上腺轴参与抑郁症的神经生物学。
IF 4.6 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-08-22 DOI: 10.1016/j.neuropharm.2024.110129
Da-Wei Xu , Wei-Yu Li , Tian-Shun Shi , Cheng-Niu Wang , Si-Yi Zhou , Wei Liu , Wei-Jia Chen , Bao-Lun Zhu , Hao Fei , Dong-dong Cheng , Zhi-Ming Cui , Bo Jiang

Hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis during chronic stress is essential for the pathogenesis of depression, and increased activity of cAMP response element binding protein (CREB)-regulated transcription co-activator 1 (CRTC1) in the paraventricular nucleus (PVN) plays a critical role. As a well-investigated microRNA (miRNA), miR-184 has two forms, miR-184-3p and miR-184-5p. Recently, miRNAs target genes predictive analysis and dual-luciferase reporter assays identified an inhibitory role of miR-184-3p on CRTC1 expression. Therefore, we speculated that miR-184-3p regulation was responsible for the effects of chronic stress on CRTC1 in the PVN. Various methods, including the chronic social defeat stress (CSDS) model of depression, behavioral tests, Western blotting, co-immunoprecipitation (Co-IP), quantitative real-time reverse transcription PCR (qRT-PCR), immunofluorescence, and adeno-associated virus (AAV)-mediated gene transfer, were used. CSDS evidently downregulated the level of miR-184-3p, but not miR-184-5p, in the PVN. Genetic knockdown and pharmacological inhibition of miR-184-3p in the PVN induced various depressive-like symptoms (e.g., abnormal behaviors, HPA hyperactivity, enhanced CRTC1 function in PVN neurons, downregulation of hippocampal neurogenesis, and decreased brain-derived neurotrophic factor (BDNF) signaling) in naïve male C57BL/6J mice. In contrast, genetic overexpression and pharmacological activation of miR-184-3p in the PVN produced significant beneficial effects against CSDS. MiR-184-3p in the PVN was necessary for the antidepressant actions of two well-known SSRIs, fluoxetine and paroxetine. Collectively. miR-184-3p was also implicated in the neurobiology of depression and may be a viable target for novel antidepressants.

慢性应激时下丘脑-垂体-肾上腺(HPA)轴的过度活跃是抑郁症发病机制的关键,而脑室旁核(PVN)中由 cAMP 反应元件结合蛋白(CREB)调控的转录共激活因子 1(CRTC1)活性的增加起着至关重要的作用。作为一种已被广泛研究的微小RNA(miRNA),miR-184有两种形式,即miR-184-3p和miR-184-5p。最近,miRNAs 靶基因预测分析和双荧光素酶报告实验确定了 miR-184-3p 对 CRTC1 表达的抑制作用。因此,我们推测miR-184-3p调控是慢性应激影响PVN中CRTC1的原因。我们采用了多种方法,包括慢性社会挫败应激(CSDS)抑郁模型、行为测试、Western印迹、共免疫沉淀(Co-IP)、定量实时逆转录PCR(qRT-PCR)、免疫荧光和腺相关病毒(AAV)介导的基因转移。CSDS明显下调了PVN中miR-184-3p的水平,但没有下调miR-184-5p的水平。基因敲除和药物抑制 PVN 中的 miR-184-3p,可诱导天真雄性 C57BL/6J 小鼠出现各种类似抑郁症的症状(如行为异常、HPA 过度活跃、PVN 神经元中 CRTC1 功能增强、海马神经发生下调和脑源性神经营养因子(BDNF)信号传导减少)。与此相反,miR-184-3p在PVN中的遗传过表达和药物激活对CSDS产生了显著的有益影响。PVN中的miR-184-3p对两种著名的SSRIs(氟西汀和帕罗西汀)的抗抑郁作用是必需的。总之,miR-184-3p 也与抑郁症的神经生物学有关,可能是新型抗抑郁药物的一个可行靶点。
{"title":"MiR-184-3p in the paraventricular nucleus participates in the neurobiology of depression via regulation of the hypothalamus-pituitary-adrenal axis","authors":"Da-Wei Xu ,&nbsp;Wei-Yu Li ,&nbsp;Tian-Shun Shi ,&nbsp;Cheng-Niu Wang ,&nbsp;Si-Yi Zhou ,&nbsp;Wei Liu ,&nbsp;Wei-Jia Chen ,&nbsp;Bao-Lun Zhu ,&nbsp;Hao Fei ,&nbsp;Dong-dong Cheng ,&nbsp;Zhi-Ming Cui ,&nbsp;Bo Jiang","doi":"10.1016/j.neuropharm.2024.110129","DOIUrl":"10.1016/j.neuropharm.2024.110129","url":null,"abstract":"<div><p>Hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis during chronic stress is essential for the pathogenesis of depression, and increased activity of cAMP response element binding protein (CREB)-regulated transcription co-activator 1 (CRTC1) in the paraventricular nucleus (PVN) plays a critical role. As a well-investigated microRNA (miRNA), miR-184 has two forms, miR-184-3p and miR-184-5p. Recently, miRNAs target genes predictive analysis and dual-luciferase reporter assays identified an inhibitory role of miR-184-3p on CRTC1 expression. Therefore, we speculated that miR-184-3p regulation was responsible for the effects of chronic stress on CRTC1 in the PVN. Various methods, including the chronic social defeat stress (CSDS) model of depression, behavioral tests, Western blotting, co-immunoprecipitation (Co-IP), quantitative real-time reverse transcription PCR (qRT-PCR), immunofluorescence, and adeno-associated virus (AAV)-mediated gene transfer, were used. CSDS evidently downregulated the level of miR-184-3p, but not miR-184-5p, in the PVN. Genetic knockdown and pharmacological inhibition of miR-184-3p in the PVN induced various depressive-like symptoms (e.g., abnormal behaviors, HPA hyperactivity, enhanced CRTC1 function in PVN neurons, downregulation of hippocampal neurogenesis, and decreased brain-derived neurotrophic factor (BDNF) signaling) in naïve male C57BL/6J mice. In contrast, genetic overexpression and pharmacological activation of miR-184-3p in the PVN produced significant beneficial effects against CSDS. MiR-184-3p in the PVN was necessary for the antidepressant actions of two well-known SSRIs, fluoxetine and paroxetine. Collectively. miR-184-3p was also implicated in the neurobiology of depression and may be a viable target for novel antidepressants.</p></div>","PeriodicalId":19139,"journal":{"name":"Neuropharmacology","volume":"260 ","pages":"Article 110129"},"PeriodicalIF":4.6,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioinformatics and validation reveal the potential target of curcumin in the treatment of diabetic peripheral neuropathy 生物信息学和验证揭示姜黄素治疗糖尿病周围神经病变的潜在靶点
IF 4.6 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-08-22 DOI: 10.1016/j.neuropharm.2024.110131
Ziqiang Lin , Suo Wang , Yu Cao , Jialing Lin , Ailing Sun , Wei Huang , Jun Zhou , Qingxiong Hong

Diabetic peripheral neuropathy (DPN) is a common nerve-damaging complication of diabetes mellitus. Effective treatments are needed to alleviate and reverse diabetes-associated damage to the peripheral nerves. Curcumin is an effective neuroprotectant that plays a protective role in DPN promoted by Schwann cells (SCs) lesions. However, the potential molecular mechanism of curcumin remains unclear. Therefore, our aim is to study the detailed molecular mechanism of curcumin-mediated SCs repair in order to improve the efficacy of curcumin in the clinical treatment of DPN. First, candidate target genes of curcumin in rat SC line RSC96 cells stimulated by high glucose were identified by RNA sequencing and bioinformatic analyses. Enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) was carried out by Metascape, followed by 8 algorithms on Cytoscape to determine 4 hub genes, namly Hmox1, Pten, Vegfa and Myc. Next, gene set enrichment analysis (GSEA) and Pearson function showed that Hmox1 was significantly correlated with apoptosis. Subsequently, qRT-PCR, MTT assay, flow cytometry, caspase-3 activity detection and westernblot showed that curcumin treatment increased RSC96 cell viability, reduced cell apoptosis, increased Hmox1, Pten, Vegfa and Myc expression, and up-regulated Akt phosphorylation level under high glucose environment. Finally, molecular docking predicted the binding site of curcumin to Hmox1. These results suggest that curcumin can reduce the apoptosis of SCs induced by high glucose, and Hmox1 is a potential target for curcumin. Our findings provide new insights about the mechanism of action of curcumin on SC as a potential treatment in DPN.

糖尿病周围神经病变(DPN)是糖尿病常见的神经损伤并发症。需要有效的治疗方法来减轻和逆转糖尿病对周围神经的损害。姜黄素是一种有效的神经保护剂,可在许旺细胞(SCs)病变引起的 DPN 中发挥保护作用。然而,姜黄素的潜在分子机制仍不清楚。因此,我们的目的是研究姜黄素介导SCs修复的详细分子机制,以提高姜黄素在DPN临床治疗中的疗效。首先,通过 RNA 测序和生物信息学分析,确定了姜黄素在高糖刺激下大鼠 SC 株 RSC96 细胞中的候选靶基因。利用Metascape对基因本体(GO)和京都基因组百科全书(KEGG)进行了富集分析,然后利用Cytoscape上的8种算法确定了4个中心基因,即Hmox1、Pten、Vegfa和Myc。接着,基因组富集分析(GSEA)和皮尔逊函数显示,Hmox1与细胞凋亡显著相关。随后,qRT-PCR、MTT检测、流式细胞术、Caspase-3活性检测和Westernblot显示,姜黄素处理可提高RSC96细胞活力,减少细胞凋亡,增加Hmox1、Pten、Vegfa和Myc的表达,并上调高糖环境下的Akt磷酸化水平。最后,分子对接预测了姜黄素与 Hmox1 的结合位点。这些结果表明,姜黄素能减少高糖诱导的SCs凋亡,而Hmox1是姜黄素的一个潜在靶点。我们的研究结果为姜黄素作用于SC的机制提供了新的见解,姜黄素是治疗DPN的潜在药物。
{"title":"Bioinformatics and validation reveal the potential target of curcumin in the treatment of diabetic peripheral neuropathy","authors":"Ziqiang Lin ,&nbsp;Suo Wang ,&nbsp;Yu Cao ,&nbsp;Jialing Lin ,&nbsp;Ailing Sun ,&nbsp;Wei Huang ,&nbsp;Jun Zhou ,&nbsp;Qingxiong Hong","doi":"10.1016/j.neuropharm.2024.110131","DOIUrl":"10.1016/j.neuropharm.2024.110131","url":null,"abstract":"<div><p>Diabetic peripheral neuropathy (DPN) is a common nerve-damaging complication of diabetes mellitus. Effective treatments are needed to alleviate and reverse diabetes-associated damage to the peripheral nerves. Curcumin is an effective neuroprotectant that plays a protective role in DPN promoted by Schwann cells (SCs) lesions. However, the potential molecular mechanism of curcumin remains unclear. Therefore, our aim is to study the detailed molecular mechanism of curcumin-mediated SCs repair in order to improve the efficacy of curcumin in the clinical treatment of DPN. First, candidate target genes of curcumin in rat SC line RSC96 cells stimulated by high glucose were identified by RNA sequencing and bioinformatic analyses. Enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) was carried out by Metascape, followed by 8 algorithms on Cytoscape to determine 4 hub genes, namly Hmox1, Pten, Vegfa and Myc. Next, gene set enrichment analysis (GSEA) and Pearson function showed that Hmox1 was significantly correlated with apoptosis. Subsequently, qRT-PCR, MTT assay, flow cytometry, caspase-3 activity detection and westernblot showed that curcumin treatment increased RSC96 cell viability, reduced cell apoptosis, increased Hmox1, Pten, Vegfa and Myc expression, and up-regulated Akt phosphorylation level under high glucose environment. Finally, molecular docking predicted the binding site of curcumin to Hmox1. These results suggest that curcumin can reduce the apoptosis of SCs induced by high glucose, and Hmox1 is a potential target for curcumin. Our findings provide new insights about the mechanism of action of curcumin on SC as a potential treatment in DPN.</p></div>","PeriodicalId":19139,"journal":{"name":"Neuropharmacology","volume":"260 ","pages":"Article 110131"},"PeriodicalIF":4.6,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Neuropharmacology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1