This paper reports on novel and efficient enhancement effects of fruit juice aroma using immobilized β-glucosidase, the enzyme involved in important functions in living organisms, onto superparamagnetic nanoparticles Fe3O4 via carbodiimide β-glucosidase was purified from mandarin (Citrus reticulata) using ammonium sulfate precipitation and hydrophobic interaction chromatography. To be used in this study, superparamagnetic nanoparticles were synthesized and then the shape, size, and magnetism properties of the nanoparticles were characterized. The purified enzyme was immobilized on the nanoparticles. The optimum temperature for β-glucosidase (40 ℃) was increased by 10 ℃ after immobilization, while the optimum pH values of free and immobilized β-glucosidase were 5.5. While the Km and Vmax values of the free enzyme were 0.264 mM and 294 EU, immobilized enzyme’s Km and Vmax were 0.222 mM and 370 EU, respectively. In addition, it was determined that the storage stability of the immobilized enzyme was higher than the free enzyme. When the effect of some metal ions on the enzyme activity was examined, it was observed that Fe+2 increased the enzyme activity while other metals inhibited it. According to the results obtained, the immobilized enzyme had a flavor-enhancing effect on mandarin juice.
{"title":"Immobilization of beta-glucosidase purified from mandarin (Citrus reticulata) fruit to superparamagnetic nanoparticles and its aroma quality enhancing effect","authors":"M. Acar, Y. Turan, Olcay Sinan, S. Sinan","doi":"10.34135/nbc.1452","DOIUrl":"https://doi.org/10.34135/nbc.1452","url":null,"abstract":"This paper reports on novel and efficient enhancement effects of fruit juice aroma using immobilized β-glucosidase, the enzyme involved in important functions in living organisms, onto superparamagnetic nanoparticles Fe3O4 via carbodiimide β-glucosidase was purified from mandarin (Citrus reticulata) using ammonium sulfate precipitation and hydrophobic interaction chromatography. To be used in this study, superparamagnetic nanoparticles were synthesized and then the shape, size, and magnetism properties of the nanoparticles were characterized. The purified enzyme was immobilized on the nanoparticles. The optimum temperature for β-glucosidase (40 ℃) was increased by 10 ℃ after immobilization, while the optimum pH values of free and immobilized β-glucosidase were 5.5. While the Km and Vmax values of the free enzyme were 0.264 mM and 294 EU, immobilized enzyme’s Km and Vmax were 0.222 mM and 370 EU, respectively. In addition, it was determined that the storage stability of the immobilized enzyme was higher than the free enzyme. When the effect of some metal ions on the enzyme activity was examined, it was observed that Fe+2 increased the enzyme activity while other metals inhibited it. According to the results obtained, the immobilized enzyme had a flavor-enhancing effect on mandarin juice.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44744556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meryem Mokrani, A. Zellagui, Widad Hadjab, M. Öztürk, Ozgur Ceylen, C. Bensouici
This research aims to analyse the richness of propolis ethanolic extract from Kherrata (EEPKh) in phenolic and flavonoid compounds and its antioxidant effects using different methods. Further investigations were conducted to evaluate the antibacterial and antibiofilm potential of propolis against Enterococcus faecalis strains originated from oral diseases. The antiquorum-sensing ability against Chromobacterium violaceum CV 026 was also investigated. The results revealed that ethanolic extract contains a high content of phenolic and flavonoid compounds, with an amount of 734.3 9± 11.54µg GAE.mg-1 of Extract and 224.30 ± 0µg QE.mg-1of extract respectively. Caffeic acid (23.79 mg.g-1), hesperetin (15.42 mg.g-1), cynarin (7.59 mg.g-1), apigenin (5.91 mg.g-1), naringenin (4.90 mg.g-1), and kaempferol (3.43 mg.g-1) were identified as the major compounds by the HPLC-DAD analysis. The antioxidant activity showed good scavenging and reducing abilities. Furthermore, EEPKh demonstrated high antibacterial potency against E. faecalis strain 2 at concentration 20 mg.mL-1 with an inhibition diameter of 20.33 ± 0.57 mm. The MIC and MBC values were found to range between 0.625 and 10 mg.mL-1. Biofilm formation by E. faecalis strains was inhibited at MIC with a percentage ranging from 65.93 ± 1.11 to 51.54 ± 0.81%. Quorum sensing mechanisms in CV 026 was inhibited by EEPKh, with diameter zone of 11.16 ± 0.29 mm at MIC. This study indicated that propolis extract is considered as a new source of natural medication with therapeutic potential against oral pathology caused by free radicals, E. faecalis, biofilm formation and quorum-sensing.
{"title":"Bioactive molecules from Algerian propolis with therapeutic potential against the oral pathogen Enterococcus faecalis and Chromobacterium violaceum CV026","authors":"Meryem Mokrani, A. Zellagui, Widad Hadjab, M. Öztürk, Ozgur Ceylen, C. Bensouici","doi":"10.34135/nbc.1599","DOIUrl":"https://doi.org/10.34135/nbc.1599","url":null,"abstract":"This research aims to analyse the richness of propolis ethanolic extract from Kherrata (EEPKh) in phenolic and flavonoid compounds and its antioxidant effects using different methods. Further investigations were conducted to evaluate the antibacterial and antibiofilm potential of propolis against Enterococcus faecalis strains originated from oral diseases. The antiquorum-sensing ability against Chromobacterium violaceum CV 026 was also investigated. The results revealed that ethanolic extract contains a high content of phenolic and flavonoid compounds, with an amount of 734.3 9± 11.54µg GAE.mg-1 of Extract and 224.30 ± 0µg QE.mg-1of extract respectively. Caffeic acid (23.79 mg.g-1), hesperetin (15.42 mg.g-1), cynarin (7.59 mg.g-1), apigenin (5.91 mg.g-1), naringenin (4.90 mg.g-1), and kaempferol (3.43 mg.g-1) were identified as the major compounds by the HPLC-DAD analysis. The antioxidant activity showed good scavenging and reducing abilities. Furthermore, EEPKh demonstrated high antibacterial potency against E. faecalis strain 2 at concentration 20 mg.mL-1 with an inhibition diameter of 20.33 ± 0.57 mm. The MIC and MBC values were found to range between 0.625 and 10 mg.mL-1. Biofilm formation by E. faecalis strains was inhibited at MIC with a percentage ranging from 65.93 ± 1.11 to 51.54 ± 0.81%. Quorum sensing mechanisms in CV 026 was inhibited by EEPKh, with diameter zone of 11.16 ± 0.29 mm at MIC. This study indicated that propolis extract is considered as a new source of natural medication with therapeutic potential against oral pathology caused by free radicals, E. faecalis, biofilm formation and quorum-sensing.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45616701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sangavi Gopalakrishnan, Harini Rajkumar, U. Antony, Lakshmi B.S., N. Ganesan
Vitamin B12, a water-soluble vitamin, plays a vital role in regulating metabolism in many organisms. However, its synthesis is restricted to limited bacteria and archaea. Therefore, industrial microbial fermentation is considered as a robust method to meet the demands of the vitamin B12. Lactobacillus reuteri DSM 20016 is one of the vitamin B12 producers. In the present study, liquid paneer whey is used as the medium along with the supplements, glycerol, and cobalt chloride to enhance the vitamin B12 production. The effect of liquid paneer whey, yeast extract, glycerol, and cobalt chloride on the vitamin B12 production was further optimized using Box Behnken design. Under optimized conditions, the production of the vitamin B12 reached up to 213.449 μg.L-1. The current study validated the feasibility of utilizing liquid paneer whey as a medium to produce high yield vitamin B12 by Lactobacillus reuteri DSM 20016.
{"title":"Enhanced vitamin B12 production using paneer whey as culture medium","authors":"Sangavi Gopalakrishnan, Harini Rajkumar, U. Antony, Lakshmi B.S., N. Ganesan","doi":"10.34135/nbc.1339","DOIUrl":"https://doi.org/10.34135/nbc.1339","url":null,"abstract":"Vitamin B12, a water-soluble vitamin, plays a vital role in regulating metabolism in many organisms. However, its synthesis is restricted to limited bacteria and archaea. Therefore, industrial microbial fermentation is considered as a robust method to meet the demands of the vitamin B12. Lactobacillus reuteri DSM 20016 is one of the vitamin B12 producers. In the present study, liquid paneer whey is used as the medium along with the supplements, glycerol, and cobalt chloride to enhance the vitamin B12 production. The effect of liquid paneer whey, yeast extract, glycerol, and cobalt chloride on the vitamin B12 production was further optimized using Box Behnken design. Under optimized conditions, the production of the vitamin B12 reached up to 213.449 μg.L-1. The current study validated the feasibility of utilizing liquid paneer whey as a medium to produce high yield vitamin B12 by Lactobacillus reuteri DSM 20016.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42845814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bacteria form a biofilm that attaches the microbial population to a solid surface, thus acting as a barrier to protect external stresses from the bacterial community. In the food industry, biofilms are believed to be responsible for food-borne diseases and food spoilages. This research was conducted to characterize anti-quorum sensing (QS), anti-biofilm activity of crude extract, and supernatant from Actinobacteria isolates against biofilm of foodborne, and food spoilage bacteria. Actinobacteria is the group of filamentous spore-forming Gram-positive bacteria, which is well recognized as a source for novel secondary metabolites, such as anti-biofilm and anti-quorum sensing. Two isolates of Actinobacteria utilized in this study were recovered from the Indonesian marine environment in the previous study. Both isolates demonstrated anti-QS activity on early detection assay using the agar overlay method. The Actinobacteria isolates were fermented, and the crude extracts were obtained through extraction using 100 % ethyl acetate and further evaporation, while the supernatant was collected directly following fermentation. The entire Actinobacteria isolates displayed capability as an anti-biofilm agent in crude extracts form at a concentration of 20 mg.mL-1, alongside when in its supernatant form. The Actinobacteria isolates in both extracts and supernatants form demonstrated anti-QS activity based on the N-Hexanoyl-L- homoserine lactone (HHL) validation assay and were also categorized as non-toxic based on Brine Shrimp Lethality Assay (BSLA).
{"title":"Antibiofilm activities of crude extract and supernatant of Actinobacteria against biofilm formed by foodborne pathogens and food spoilage bacteria","authors":"Faustina Audrey Agatha, Diana Elizabeth Waturangi","doi":"10.34135/nbc.1283","DOIUrl":"https://doi.org/10.34135/nbc.1283","url":null,"abstract":"Bacteria form a biofilm that attaches the microbial population to a solid surface, thus acting as a barrier to protect external stresses from the bacterial community. In the food industry, biofilms are believed to be responsible for food-borne diseases and food spoilages. This research was conducted to characterize anti-quorum sensing (QS), anti-biofilm activity of crude extract, and supernatant from Actinobacteria isolates against biofilm of foodborne, and food spoilage bacteria. Actinobacteria is the group of filamentous spore-forming Gram-positive bacteria, which is well recognized as a source for novel secondary metabolites, such as anti-biofilm and anti-quorum sensing. Two isolates of Actinobacteria utilized in this study were recovered from the Indonesian marine environment in the previous study. Both isolates demonstrated anti-QS activity on early detection assay using the agar overlay method. The Actinobacteria isolates were fermented, and the crude extracts were obtained through extraction using 100 % ethyl acetate and further evaporation, while the supernatant was collected directly following fermentation. The entire Actinobacteria isolates displayed capability as an anti-biofilm agent in crude extracts form at a concentration of 20 mg.mL-1, alongside when in its supernatant form. The Actinobacteria isolates in both extracts and supernatants form demonstrated anti-QS activity based on the N-Hexanoyl-L- homoserine lactone (HHL) validation assay and were also categorized as non-toxic based on Brine Shrimp Lethality Assay (BSLA).","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135206991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Mphahlele, N. Ngqwala, Oscar Agbor Ambang, C. S. Srinivas, R. Tandlich
Energy drinks (EDs) have been available at the global market for almost 100 years and today, they are advertised as ’tools’ boost energy, enhance physical performance and mental alertness. Some of the main chemical components in EDs include caffeine, ginseng, and taurine. The market and consumption of these beverages is growing exponentially, and this is becoming a public health problem due to the adverse effects associated with these drinks. The main objective of this article is to review important ingredients in popular EDs in South Africa and look at their molecular mechanisms of action and interaction with other compounds within the body. At the same time, the authors aim to review the global consumption pattern of EDs among children and adolescents. Finally, this review article will provide an overview the health risks associated with EDs consumption. A literature review was conducted using scholarly databases. Keywords such as energy drinks, adverse effects, advertising, alcohol, caffeine, taurine, and regulations were used. Despite the claims of having significant benefits to mental and physical stamina, long-term consumption of EDs could have detrimental public health implications; and could result in increasing rates of the central nervous system disorders and the cardiovascular complications. A knowledge gap exists on how the lack of education impacts the decision of consumers of EDs and the parental guidance provided to children and adolescents in relation to the consumption of these beverages in South Africa. To address the issue of excessive ED consumption, communities need to be made aware of the harmful effects of these beverages through health education and implementing strict regulatory measures through policy.
{"title":"Health risks associated with the chronic consumption of energy drinks in children and adolescents","authors":"K. Mphahlele, N. Ngqwala, Oscar Agbor Ambang, C. S. Srinivas, R. Tandlich","doi":"10.34135/nbc.1344","DOIUrl":"https://doi.org/10.34135/nbc.1344","url":null,"abstract":"Energy drinks (EDs) have been available at the global market for almost 100 years and today, they are advertised as ’tools’ boost energy, enhance physical performance and mental alertness. Some of the main chemical components in EDs include caffeine, ginseng, and taurine. The market and consumption of these beverages is growing exponentially, and this is becoming a public health problem due to the adverse effects associated with these drinks. The main objective of this article is to review important ingredients in popular EDs in South Africa and look at their molecular mechanisms of action and interaction with other compounds within the body. At the same time, the authors aim to review the global consumption pattern of EDs among children and adolescents. Finally, this review article will provide an overview the health risks associated with EDs consumption. A literature review was conducted using scholarly databases. Keywords such as energy drinks, adverse effects, advertising, alcohol, caffeine, taurine, and regulations were used. Despite the claims of having significant benefits to mental and physical stamina, long-term consumption of EDs could have detrimental public health implications; and could result in increasing rates of the central nervous system disorders and the cardiovascular complications. A knowledge gap exists on how the lack of education impacts the decision of consumers of EDs and the parental guidance provided to children and adolescents in relation to the consumption of these beverages in South Africa. To address the issue of excessive ED consumption, communities need to be made aware of the harmful effects of these beverages through health education and implementing strict regulatory measures through policy.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43988759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jorge Olivera Rodi, Marcela Joan Gonz´ález Ramos, Pedro Díaz Gadea, Stella M. Reginensi
Three strains of Lactobacillus were previously isolated from dairy products, Lactobacillus casei 26, Lactobacillus delbrueckii subsp. bulgaricus 76 and L. casei 95. Anti-costridial activities of these strains were evaluated against a collection of Clostridium isolates, belonging to species responsible for late blowing defect in cheese. The three strains of Lactobacillus inhibited the growth of all Clostridium isolates analyzed. The inhibitory effect of cell-free supernatant of each of these Lactobacillus strains combined with that of commercial strain L. casei BAL C, or with a solution of lysozyme, were compared by disc diffusion assay. Mixtures of cell-free Lactobacillus supernatants and lysozyme exhibited higher inhibitory activity than the supernatants and lysozyme solution separately (P < 0.05). Additionally, Lactobacillus strains were resistant to lysozyme concentrations usually used during cheese making process.
从乳制品中分离到3株乳酸菌，分别为干酪乳杆菌26、德尔布鲁氏乳杆菌亚种。保加利亚菌76和干酪菌95。这些菌株的抗虫瘟活性与一系列梭状芽孢杆菌分离株进行了比较，这些梭状芽孢杆菌属于导致奶酪晚吹缺陷的物种。三株乳酸菌均能抑制梭状芽孢杆菌的生长。采用圆盘扩散法比较各组乳酸菌无细胞上清液与商业菌株L. casei BAL C或溶菌酶溶液的抑菌效果。无细胞乳酸菌上清液与溶菌酶混合的抑菌活性高于上清液和溶菌酶溶液(P < 0.05)。此外，乳杆菌菌株对奶酪制作过程中常用的溶菌酶浓度具有抗性。
{"title":"Study of the inhibitory effect of Lactobacillus strains and lysozyme on growth of Clostridium spp. responsible for cheese late blowing defect","authors":"Jorge Olivera Rodi, Marcela Joan Gonz´ález Ramos, Pedro Díaz Gadea, Stella M. Reginensi","doi":"10.34135/nbc.1229","DOIUrl":"https://doi.org/10.34135/nbc.1229","url":null,"abstract":"Three strains of Lactobacillus were previously isolated from dairy products, Lactobacillus casei 26, Lactobacillus delbrueckii subsp. bulgaricus 76 and L. casei 95. Anti-costridial activities of these strains were evaluated against a collection of Clostridium isolates, belonging to species responsible for late blowing defect in cheese. The three strains of Lactobacillus inhibited the growth of all Clostridium isolates analyzed. The inhibitory effect of cell-free supernatant of each of these Lactobacillus strains combined with that of commercial strain L. casei BAL C, or with a solution of lysozyme, were compared by disc diffusion assay. Mixtures of cell-free Lactobacillus supernatants and lysozyme exhibited higher inhibitory activity than the supernatants and lysozyme solution separately (P < 0.05). Additionally, Lactobacillus strains were resistant to lysozyme concentrations usually used during cheese making process.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44528134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bacteria form a biofilm that attaches the microbial population to a solid surface, thus acting as a barrier to protect external stresses from the bacterial community. In the food industry, biofilms are believed to be responsible for food-borne diseases and food spoilages. This research was conducted to characterize anti-quorum sensing (QS), anti-biofilm activity of crude extract, and supernatant from Actinobacteria isolates against biofilm of foodborne, and food spoilage bacteria. Actinobacteria is the group of filamentous spore-forming Gram-positive bacteria, which is well recognized as a source for novel secondary metabolites, such as anti-biofilm and anti-quorum sensing. Two isolates of Actinobacteria utilized in this study were recovered from the Indonesian marine environment in the previous study. Both isolates demonstrated anti-QS activity on early detection assay using the agar overlay method. The Actinobacteria isolates were fermented, and the crude extracts were obtained through extraction using 100 % ethyl acetate and further evaporation, while the supernatant was collected directly following fermentation. The entire Actinobacteria isolates displayed capability as an anti-biofilm agent in crude extracts form at a concentration of 20 mg.mL-1, alongside when in its supernatant form. The Actinobacteria isolates in both extracts and supernatants form demonstrated anti-QS activity based on the N-Hexanoyl-L- homoserine lactone (HHL) validation assay and were also categorized as non-toxic based on Brine Shrimp Lethality Assay (BSLA).
{"title":"Antibiofilm activities of crude extract and supernatant of Actinobacteria against biofilm formed by foodborne pathogens and food spoilage bacteria","authors":"F. Agatha, D. Waturangi","doi":"10.36547/nbc.1283","DOIUrl":"https://doi.org/10.36547/nbc.1283","url":null,"abstract":"Bacteria form a biofilm that attaches the microbial population to a solid surface, thus acting as a barrier to protect external stresses from the bacterial community. In the food industry, biofilms are believed to be responsible for food-borne diseases and food spoilages. This research was conducted to characterize anti-quorum sensing (QS), anti-biofilm activity of crude extract, and supernatant from Actinobacteria isolates against biofilm of foodborne, and food spoilage bacteria. Actinobacteria is the group of filamentous spore-forming Gram-positive bacteria, which is well recognized as a source for novel secondary metabolites, such as anti-biofilm and anti-quorum sensing. Two isolates of Actinobacteria utilized in this study were recovered from the Indonesian marine environment in the previous study. Both isolates demonstrated anti-QS activity on early detection assay using the agar overlay method. The Actinobacteria isolates were fermented, and the crude extracts were obtained through extraction using 100 % ethyl acetate and further evaporation, while the supernatant was collected directly following fermentation. The entire Actinobacteria isolates displayed capability as an anti-biofilm agent in crude extracts form at a concentration of 20 mg.mL-1, alongside when in its supernatant form. The Actinobacteria isolates in both extracts and supernatants form demonstrated anti-QS activity based on the N-Hexanoyl-L- homoserine lactone (HHL) validation assay and were also categorized as non-toxic based on Brine Shrimp Lethality Assay (BSLA).","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42363794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Timotej Jankech, J. Sokol, M. Maliarová, Ivana Gerhardtová, Nicholas Martinka, Marcela Blažková, M. Havrlentová
Phenolic amides contained in oats (Avenanthramides; AVNs) are biologically active substances with strong antioxidant activity. In this paper, we evaluated efficiency of three C18 chromatographic columns (Symmetry, XBridge, Cortecs) with different particle technology and particle sizes for the separation of three major avenanthramides (AVN A, AVN B, AVN C). We compared columns in terms of retention times, retention factors of AVNs and in terms of other parameters such as number of theoretical plates, height equivalent to a theoretical plate, reduced plate height, resolution and in terms of peak symmetry, respectively. Limits of detection and limits of quantification of AVNs on all columns were calculated. Retention results of AVNs on individual columns showed a significant reduction in retention times of AVNs on solid core column with a particle size 2.7 μm compared to columns with particle size 3.5 μm. Within columns with 3.5 μm particles, separation on Symmetry C18 column appeared to be more efficient than on the XBridge C18 column. In general, results achieved on Cortecs C18 column can be considered as the best in terms of both separation efficiency and retention times.
{"title":"Evaluation of chromatographic performance of three C18 columns for avenanthramides separation","authors":"Timotej Jankech, J. Sokol, M. Maliarová, Ivana Gerhardtová, Nicholas Martinka, Marcela Blažková, M. Havrlentová","doi":"10.36547/nbc.1483","DOIUrl":"https://doi.org/10.36547/nbc.1483","url":null,"abstract":"Phenolic amides contained in oats (Avenanthramides; AVNs) are biologically active substances with strong antioxidant activity. In this paper, we evaluated efficiency of three C18 chromatographic columns (Symmetry, XBridge, Cortecs) with different particle technology and particle sizes for the separation of three major avenanthramides (AVN A, AVN B, AVN C). We compared columns in terms of retention times, retention factors of AVNs and in terms of other parameters such as number of theoretical plates, height equivalent to a theoretical plate, reduced plate height, resolution and in terms of peak symmetry, respectively. Limits of detection and limits of quantification of AVNs on all columns were calculated. Retention results of AVNs on individual columns showed a significant reduction in retention times of AVNs on solid core column with a particle size 2.7 μm compared to columns with particle size 3.5 μm. Within columns with 3.5 μm particles, separation on Symmetry C18 column appeared to be more efficient than on the XBridge C18 column. In general, results achieved on Cortecs C18 column can be considered as the best in terms of both separation efficiency and retention times.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45032335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Pažitný, A. Russ, Š. Boháček, M. Halaj, Š. Šutý, Ida Skotnicová, V. Ihnát
Waste dendromass is one of the least used types of biomass and together with waste from the harvesting and processing of agricultural crops, belongs to the wastes that have a high potential for the second-generation ethanol production. Mass concentration of biofuel added to fossil fuels is established by law and this concentration is determined within the reference values for each year. For example, for 2021 it was 8.0 % and for years from 2022 to 2030 the value will increase to 8.2 %. Biofuels in the so-called reference value in the total fuel content have been mandatory since 2017, while the specific reference value is calculated from the energy content of the total amount of fuels placed on the market. This paper provides initial considerations and pilot results based on experimental data obtained in our workplace concerning the application potential of selected type of dendromass – beech particles from the point of view of their pretreatment by steam explosion and steam extrusion for potential production of liquid biofuels based on lignocellulosic biomass. We compared temperature courses of the mentioned pretreatment methods and pretreated samples were tested by enzymatic hydrolysis. Enzymatic hydrolysis results showed that steam explosion and steam extrusion led to concentration enhancement of fermentable monosaccharides compared to original samples depending on used pretreatment method.
{"title":"New approaches in pretreatment methods of beech particles in enzymatic hydrolysis – effect of impregnation time and reactor selection","authors":"A. Pažitný, A. Russ, Š. Boháček, M. Halaj, Š. Šutý, Ida Skotnicová, V. Ihnát","doi":"10.36547/nbc.1247","DOIUrl":"https://doi.org/10.36547/nbc.1247","url":null,"abstract":"Waste dendromass is one of the least used types of biomass and together with waste from the harvesting and processing of agricultural crops, belongs to the wastes that have a high potential for the second-generation ethanol production. Mass concentration of biofuel added to fossil fuels is established by law and this concentration is determined within the reference values for each year. For example, for 2021 it was 8.0 % and for years from 2022 to 2030 the value will increase to 8.2 %. Biofuels in the so-called reference value in the total fuel content have been mandatory since 2017, while the specific reference value is calculated from the energy content of the total amount of fuels placed on the market. This paper provides initial considerations and pilot results based on experimental data obtained in our workplace concerning the application potential of selected type of dendromass – beech particles from the point of view of their pretreatment by steam explosion and steam extrusion for potential production of liquid biofuels based on lignocellulosic biomass. We compared temperature courses of the mentioned pretreatment methods and pretreated samples were tested by enzymatic hydrolysis. Enzymatic hydrolysis results showed that steam explosion and steam extrusion led to concentration enhancement of fermentable monosaccharides compared to original samples depending on used pretreatment method.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42726393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hong Thien Van, Tan Viet Pham, Ngoc An Nguyen, Hanh Thi Dieu Nguyen, Quoc Hung Nguyen, Thanh Tho Le, Van Son Le
Globba macrocarpa Gagnep. is a rare species of Globba genus (Zingiberaceae family). The present study reported the chemical compositions and antibacterial effects of acetone extracts obtained from the G. macrocarpa rhizomes and aerial parts. By using Gas Chromatography Mass Spectrometry (GC/MS) assay, fifty and thirty-two chemical compounds were identified from rhizomes and aerial parts of the species, respectively. Of those, germacrene D (15.25 %), 1H-indole, 4-(3-methyl-2-butenyl)- (14.33 %), (E)-β-farnesene (11.28 %), and 2-biphenylamine, 3-methyl (10.27 %) were the major constituents in the rhizome extract while the aerial part extract was characterized by the predominance of linolenic acid (19.89 %), palmitic acid (13.05 %), phytol (7.52 %), and neophytadiene (4.76 %). In addition, the rhizome extract had antibacterial activities against five 5 out of 6 oral bacteria, including Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium Bacillus cereus, and Staphylococcus aureus. Meanwhile, the aerial part extract was active against 4 out of 6 test bacteria, except for Escherichia coli and S. typhimurium.
{"title":"Chemical profiles and antibacterial activities of acetone extracts of Globba macrocarpa","authors":"Hong Thien Van, Tan Viet Pham, Ngoc An Nguyen, Hanh Thi Dieu Nguyen, Quoc Hung Nguyen, Thanh Tho Le, Van Son Le","doi":"10.36547/nbc.1322","DOIUrl":"https://doi.org/10.36547/nbc.1322","url":null,"abstract":"Globba macrocarpa Gagnep. is a rare species of Globba genus (Zingiberaceae family). The present study reported the chemical compositions and antibacterial effects of acetone extracts obtained from the G. macrocarpa rhizomes and aerial parts. By using Gas Chromatography Mass Spectrometry (GC/MS) assay, fifty and thirty-two chemical compounds were identified from rhizomes and aerial parts of the species, respectively. Of those, germacrene D (15.25 %), 1H-indole, 4-(3-methyl-2-butenyl)- (14.33 %), (E)-β-farnesene (11.28 %), and 2-biphenylamine, 3-methyl (10.27 %) were the major constituents in the rhizome extract while the aerial part extract was characterized by the predominance of linolenic acid (19.89 %), palmitic acid (13.05 %), phytol (7.52 %), and neophytadiene (4.76 %). In addition, the rhizome extract had antibacterial activities against five 5 out of 6 oral bacteria, including Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium Bacillus cereus, and Staphylococcus aureus. Meanwhile, the aerial part extract was active against 4 out of 6 test bacteria, except for Escherichia coli and S. typhimurium.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49237327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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