A. Saleh, S. M. Zainal-Ariffin, S. F. Yahaya, A. Khaleel
Phytochemical analysis is important in the evaluation of bioactive compounds from plants. Oxidative stress has been implicated in the pathology of many diseases such as atherosclerosis, rheumatoid arthritis, cancer, cataract, diabetes, cardiovascular diseases, chronic inflammatory conditions, and stroke. The aim of this study was to evaluate polyphenolic content and antioxidant activity of Trema orientalis. Antioxidant activity was estimated spectrophotometrically using 2,2– diphenyl–1–picrylhydrazyl radical scavenging method. The total polyphenolic and flavonoid contents of the Trema orientalis extracts were determined using standard methods. Independent Sample T–test was used for Data analyses. Phytochemical screening revealed the presence of saponins, tannins, steroids, cardiac glycosides, alkaloids, triterpenes, flavonoids and phenolic compounds. Total phenolic contents were found to be 260.96±2.31 mg GAE/g and 134.08±0.56 mg GAE/g in the ethanol and aqueous extracts respectively. Similarly, total flavonoid contents were between 32.71±0.89 and 4.70±0.23 mg GAE/g. The radical scavenging effect was observed in ethanol extract with IC50 = 9.27 μg/mL. The abundance of polyphenolic compounds and antioxidant activities of the T. orientalis could confirm their good therapeutic potentials in ethnobotany.
{"title":"Antioxidant Activities and Estimation of Phenol and Flavonoid Contents in The Extracts Of Trema Orientalis Linn Blume","authors":"A. Saleh, S. M. Zainal-Ariffin, S. F. Yahaya, A. Khaleel","doi":"10.4314/NVJ.V41I2.1","DOIUrl":"https://doi.org/10.4314/NVJ.V41I2.1","url":null,"abstract":"Phytochemical analysis is important in the evaluation of bioactive compounds from plants. Oxidative stress has been implicated in the pathology of many diseases such as atherosclerosis, rheumatoid arthritis, cancer, cataract, diabetes, cardiovascular diseases, chronic inflammatory conditions, and stroke. The aim of this study was to evaluate polyphenolic content and antioxidant activity of Trema orientalis. Antioxidant activity was estimated spectrophotometrically using 2,2– diphenyl–1–picrylhydrazyl radical scavenging method. The total polyphenolic and flavonoid contents of the Trema orientalis extracts were determined using standard methods. Independent Sample T–test was used for Data analyses. Phytochemical screening revealed the presence of saponins, tannins, steroids, cardiac glycosides, alkaloids, triterpenes, flavonoids and phenolic compounds. Total phenolic contents were found to be 260.96±2.31 mg GAE/g and 134.08±0.56 mg GAE/g in the ethanol and aqueous extracts respectively. Similarly, total flavonoid contents were between 32.71±0.89 and 4.70±0.23 mg GAE/g. The radical scavenging effect was observed in ethanol extract with IC50 = 9.27 μg/mL. The abundance of polyphenolic compounds and antioxidant activities of the T. orientalis could confirm their good therapeutic potentials in ethnobotany.","PeriodicalId":19330,"journal":{"name":"Nigerian Veterinary Journal","volume":"60 1","pages":"73-84"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89095853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Ularamu, J. Ibu, J. Abenga, D. Lazarus, Y. Wungak, D. Ehizibolo, D. Shamaki, M. Adah
Foot-and-mouth disease (FMD) is an endemic transboundary animal disease that affects livestock health across most of sub-Saharan Africa. Since the first official report of FMD in Nigeria in 1924, serotypes O, A, SAT 1 and SAT 2 have been documented within the country. Molecular epidemiology has been used to trace the origin of FMD outbreaks in the case of animal movement, inter-species transmissions and trans-continental introductions. Phylogenetic analyses of VP1 nucleotide sequences of the twelve isolates (n=12) provide evidence for the presence of type O/EAST AFRICA 3 (EA-3) in Nigeria. The epidemiological situation of FMD in Nigeria and other West African countries is further complicated by the emergence of the O/EA-3 lineage that is causing new outbreaks in the region in addition to the West Africa (WA) topotype that has been known to be in circulation in Nigeria and other parts of West African and Central African regions. These recent development in west and central Africa, indicates the dynamic and complex nature of FMD epidemiology in the region and this is not un-connected to the nomadism in the region. In addition the un-restricted animal movement across the porous border in the region in search of pasture and water for their animal has contributed to the spread of diseases across the region. From the results the phylogenetic analysis of the O/EA-3 has close identity and was closely related to O/EA-3 from the 2009 outbreak in Sudan. Therefore, based on these findings a sustained surveillance is required to yet understand the epidemiology of FMD in West and Central Africa that will inform the type of vaccine and target areas in other to control the disease. The need to restrict animal movement across the border should be put in place and where necessary all animals that must move from one point to another must have proper evidence of vaccine certification before it should be allow access into the area. Finally for effective FMD control, regional vaccination and surveillance should be advocated and it should be backed by law. The need to have FMD vaccination at national as well as regional FMD control policy is strongly advocate for effective FMD control in Nigeria and across the regions.
{"title":"Incursion of Foot-and-Mouth Disease (FMD) Serotype O East Africa Topotype -3 (O/EA-3) in Nigeria","authors":"H. Ularamu, J. Ibu, J. Abenga, D. Lazarus, Y. Wungak, D. Ehizibolo, D. Shamaki, M. Adah","doi":"10.4314/NVJ.V41I1.4","DOIUrl":"https://doi.org/10.4314/NVJ.V41I1.4","url":null,"abstract":"Foot-and-mouth disease (FMD) is an endemic transboundary animal disease that affects livestock health across most of sub-Saharan Africa. Since the first official report of FMD in Nigeria in 1924, serotypes O, A, SAT 1 and SAT 2 have been documented within the country. Molecular epidemiology has been used to trace the origin of FMD outbreaks in the case of animal movement, inter-species transmissions and trans-continental introductions. Phylogenetic analyses of VP1 nucleotide sequences of the twelve isolates (n=12) provide evidence for the presence of type O/EAST AFRICA 3 (EA-3) in Nigeria. The epidemiological situation of FMD in Nigeria and other West African countries is further complicated by the emergence of the O/EA-3 lineage that is causing new outbreaks in the region in addition to the West Africa (WA) topotype that has been known to be in circulation in Nigeria and other parts of West African and Central African regions. These recent development in west and central Africa, indicates the dynamic and complex nature of FMD epidemiology in the region and this is not un-connected to the nomadism in the region. In addition the un-restricted animal movement across the porous border in the region in search of pasture and water for their animal has contributed to the spread of diseases across the region. From the results the phylogenetic analysis of the O/EA-3 has close identity and was closely related to O/EA-3 from the 2009 outbreak in Sudan. Therefore, based on these findings a sustained surveillance is required to yet understand the epidemiology of FMD in West and Central Africa that will inform the type of vaccine and target areas in other to control the disease. The need to restrict animal movement across the border should be put in place and where necessary all animals that must move from one point to another must have proper evidence of vaccine certification before it should be allow access into the area. Finally for effective FMD control, regional vaccination and surveillance should be advocated and it should be backed by law. The need to have FMD vaccination at national as well as regional FMD control policy is strongly advocate for effective FMD control in Nigeria and across the regions.","PeriodicalId":19330,"journal":{"name":"Nigerian Veterinary Journal","volume":"53 1","pages":"23-31"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91359424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Alkali, S. Asuku, M. B. Umar, A. Abba, A. Mustapha, M. M. Bukar, M. Waziri
Microorganisms that inhabit the avian cloaca usually contaminate poultry semen which could easily spread throughout an entire flock. This study was conducted to determine the presence of microbial contaminants in turkey semen and evaluate their antibiotic sensitivity. Semen was collected from each tom, pooled and then divided into two aliquots A and B. Aliquot A was immediately evaluated for microbial contaminants and antibiotic sensitivity while aliquot B, was extended and preserved for 24 hours at 4 C and thereafter microbial culture, identification and antibiotic sensitivity were conducted. Escherichia coli, Enterococcus faecalis, Bacillus subtilis, Corynebacteria species and a fungal organism Candida albican were isolated and identified in both aliquots. All the identified organisms were sensitive to pefloxacin, gentamicin and ciprofloxacin, while Enterococcus faecalis, Bacillus subtilis and Corynebacteria species were resistant to Ampicillin-cloxacillin, cefuroxime, amoxicillin and ceftriaxone. Escherichia coli was only resistant to co-trimoxazole, ofloxacin and nalidixic acid. The study concludes that, Escherichia coli, Enterococcus foecalis, Bacillus subtilis, Corynebacteria species and Candida albican were found to be turkey semen contaminants and were resistant to penicillin and streptomycin combination in turkey semen extender but sensitive to pefloxacin, gentamicin and ciprofloxacin.
{"title":"Microbial Contaminants in Fresh and Extended Turkey Semen and their Sensitivity to Antibiotics","authors":"I. Alkali, S. Asuku, M. B. Umar, A. Abba, A. Mustapha, M. M. Bukar, M. Waziri","doi":"10.4314/NVJ.V41I1.1","DOIUrl":"https://doi.org/10.4314/NVJ.V41I1.1","url":null,"abstract":"Microorganisms that inhabit the avian cloaca usually contaminate poultry semen which could easily spread throughout an entire flock. This study was conducted to determine the presence of microbial contaminants in turkey semen and evaluate their antibiotic sensitivity. Semen was collected from each tom, pooled and then divided into two aliquots A and B. Aliquot A was immediately evaluated for microbial contaminants and antibiotic sensitivity while aliquot B, was extended and preserved for 24 hours at 4 C and thereafter microbial culture, identification and antibiotic sensitivity were conducted. Escherichia coli, Enterococcus faecalis, Bacillus subtilis, Corynebacteria species and a fungal organism Candida albican were isolated and identified in both aliquots. All the identified organisms were sensitive to pefloxacin, gentamicin and ciprofloxacin, while Enterococcus faecalis, Bacillus subtilis and Corynebacteria species were resistant to Ampicillin-cloxacillin, cefuroxime, amoxicillin and ceftriaxone. Escherichia coli was only resistant to co-trimoxazole, ofloxacin and nalidixic acid. The study concludes that, Escherichia coli, Enterococcus foecalis, Bacillus subtilis, Corynebacteria species and Candida albican were found to be turkey semen contaminants and were resistant to penicillin and streptomycin combination in turkey semen extender but sensitive to pefloxacin, gentamicin and ciprofloxacin.","PeriodicalId":19330,"journal":{"name":"Nigerian Veterinary Journal","volume":"40 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80585730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Imam, J. Hambolu, M. B. Umar, M. H. Sulaiman, A. D. Umosen
Eighty – seven foetal lung samples from camels (Camelus dromedarius) were collected at Kano abattoir to study lung development. Four phases of the lung development were observed as follows; pseudo-glandular phase was between 10.5 cm to 38.2 cm crown-rump length, with endodermal buds and prominent mesenchymal cells. Canalicular phase was observed between 38.7 cm to 62.2 cm crownrump length and was characterized by thinning of the endodermal buds and invasion of capillaries. Saccular phase was noticed at 65.6 cm to 82.6 cm crown-rump length when thick intercellular septa were noticed. Alveolar phase was noticed between 85.9 cm to 121 cm crown-rump length when primitive alveolar was noticed. This study has established phases of lung development in the camel and serves as baseline information for scientific knowledge.
{"title":"Microscopic Studies Of The Foetal Lung Development In The OneHumped Camel (Camelus dromedarius) Obtained From Kano Abattoir","authors":"J. Imam, J. Hambolu, M. B. Umar, M. H. Sulaiman, A. D. Umosen","doi":"10.4314/NVJ.V40I4.8","DOIUrl":"https://doi.org/10.4314/NVJ.V40I4.8","url":null,"abstract":"Eighty – seven foetal lung samples from camels (Camelus dromedarius) were collected at Kano abattoir to study lung development. Four phases of the lung development were observed as follows; pseudo-glandular phase was between 10.5 cm to 38.2 cm crown-rump length, with endodermal buds and prominent mesenchymal cells. Canalicular phase was observed between 38.7 cm to 62.2 cm crownrump length and was characterized by thinning of the endodermal buds and invasion of capillaries. Saccular phase was noticed at 65.6 cm to 82.6 cm crown-rump length when thick intercellular septa were noticed. Alveolar phase was noticed between 85.9 cm to 121 cm crown-rump length when primitive alveolar was noticed. This study has established phases of lung development in the camel and serves as baseline information for scientific knowledge.","PeriodicalId":19330,"journal":{"name":"Nigerian Veterinary Journal","volume":"19 1","pages":"326-330"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72799919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Idika, V. J. Ebuk, E. Okoro, T. Nzeakor, N. M. Uzonnah, C. Nwosu
The efficacy of Albendazole against trichostrongyle nematode parasites in goats presented for slaughter at the Nsukka municipal abattoir was evaluated using the In vitro Egg hatch assay (EHA) model. The abattoir was visited once every week for 4 consecutive months during which a total of 240 goats were sampled. Fecal samples were collected per rectum from a minimum of 15 goats on each day of the visit. Egg Hatch Assay was performed on strongyle eggs recovered from pooled faecal sample on each day of sampling with a 2.5% W/V Albendazole. Faecal culture was also set up from the pooled faecal sample on each sampling day to recover and identify the nematode parasites present in the goats. Among the 240 goats sampled, the prevalence of trichostrongylosis as observed by the presence of strongyle eggs was 94.6% (227/240). Faecal culture and larval identification revealed 69.8% of the strongyles as Haemonchus contortus, while 25.5 and 4.8% were Trichostrongylus colubriformis and Oesophagostomum species respectively. In the EHA, Albendazole had mean LC50 value of 0.16 μg/ml which is slightly in excess of the discriminating dose of 0.1μg/ml as prescribed by the World Association for the Advancement of Veterinary Parasitology (WAAVP) as an indication of anthelmintic resistance. There is therefore an urgent need to screen the nematode parasite population in the Nigeria for the presence Albendazole resistance genes.
{"title":"In vitro Efficacy of Albendazole against Strongyle eggs recovered from Trade Goats slaughtered at the Nsukka Abattoir: A Preliminary Survey of Resistance to Albendazole","authors":"I. Idika, V. J. Ebuk, E. Okoro, T. Nzeakor, N. M. Uzonnah, C. Nwosu","doi":"10.4314/NVJ.V40I4.4","DOIUrl":"https://doi.org/10.4314/NVJ.V40I4.4","url":null,"abstract":"The efficacy of Albendazole against trichostrongyle nematode parasites in goats presented for slaughter at the Nsukka municipal abattoir was evaluated using the In vitro Egg hatch assay (EHA) model. The abattoir was visited once every week for 4 consecutive months during which a total of 240 goats were sampled. Fecal samples were collected per rectum from a minimum of 15 goats on each day of the visit. Egg Hatch Assay was performed on strongyle eggs recovered from pooled faecal sample on each day of sampling with a 2.5% W/V Albendazole. Faecal culture was also set up from the pooled faecal sample on each sampling day to recover and identify the nematode parasites present in the goats. Among the 240 goats sampled, the prevalence of trichostrongylosis as observed by the presence of strongyle eggs was 94.6% (227/240). Faecal culture and larval identification revealed 69.8% of the strongyles as Haemonchus contortus, while 25.5 and 4.8% were Trichostrongylus colubriformis and Oesophagostomum species respectively. In the EHA, Albendazole had mean LC50 value of 0.16 μg/ml which is slightly in excess of the discriminating dose of 0.1μg/ml as prescribed by the World Association for the Advancement of Veterinary Parasitology (WAAVP) as an indication of anthelmintic resistance. There is therefore an urgent need to screen the nematode parasite population in the Nigeria for the presence Albendazole resistance genes.","PeriodicalId":19330,"journal":{"name":"Nigerian Veterinary Journal","volume":"80 9","pages":"287-295"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72598312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Awoyomi, N. O. Bankole, O. Kehinde, O. Adebowale
A cross sectional study was designed to assess knowledge of dog owners towards dog rabies vaccination in rural communities in Odeda local government area of Ogun State. A structured questionnaire was administered to 268 dog owners. The questionnaire sought information on demographic characteristics of the dog owners, their association with dogs, and knowledge about dog rabies vaccine. Data obtained were analyzed using chi square and logistic regression with p value set at ≤ 0.05. Dog rabies vaccination coverage in the study area was 4.9%. Most of the respondents (67.2%) were not aware of dog rabies vaccine. Only 3% of the dog owners had good knowledge about rabies vaccination. Socio-economic factors that had significant association with knowledge level were age, occupation, educational level and marital status of the respondents. There was also significant association between age, religion, occupation, educational level, awareness on dog rabies vaccination, knowledge about dog rabies vaccination, danger posed by non vaccinated dogs to the public and other animals and the intention of dog owners to vaccinate their dogs against rabies. One hundred and eighty seven respondents (70%) declared their intention to vaccinate their dogs, although 82% affirmed willingness to pay for vaccination of their dogs against rabies; however the average amount indicated was only ₦200.00 ($0.57). In conclusion, it is free vaccination of dogs against rabies with an aggressive mass orientation on responsible dog ownership and enlightenment on fatality of clinical rabies through the mass media that can significantly alter the vaccination coverage in the studied community Key words: Rabies, vaccination, dogs, rural communities, intention
{"title":"Assessment of Dog Owners’ Knowledge on Dog Rabies Vaccination in Rural Communities in Ogun State, Nigeria","authors":"O. Awoyomi, N. O. Bankole, O. Kehinde, O. Adebowale","doi":"10.4314/NVJ.V40I4.3","DOIUrl":"https://doi.org/10.4314/NVJ.V40I4.3","url":null,"abstract":"A cross sectional study was designed to assess knowledge of dog owners towards dog rabies vaccination in rural communities in Odeda local government area of Ogun State. A structured questionnaire was administered to 268 dog owners. The questionnaire sought information on demographic characteristics of the dog owners, their association with dogs, and knowledge about dog rabies vaccine. Data obtained were analyzed using chi square and logistic regression with p value set at ≤ 0.05. Dog rabies vaccination coverage in the study area was 4.9%. Most of the respondents (67.2%) were not aware of dog rabies vaccine. Only 3% of the dog owners had good knowledge about rabies vaccination. Socio-economic factors that had significant association with knowledge level were age, occupation, educational level and marital status of the respondents. There was also significant association between age, religion, occupation, educational level, awareness on dog rabies vaccination, knowledge about dog rabies vaccination, danger posed by non vaccinated dogs to the public and other animals and the intention of dog owners to vaccinate their dogs against rabies. One hundred and eighty seven respondents (70%) declared their intention to vaccinate their dogs, although 82% affirmed willingness to pay for vaccination of their dogs against rabies; however the average amount indicated was only ₦200.00 ($0.57). In conclusion, it is free vaccination of dogs against rabies with an aggressive mass orientation on responsible dog ownership and enlightenment on fatality of clinical rabies through the mass media that can significantly alter the vaccination coverage in the studied community \u0000Key words: Rabies, vaccination, dogs, rural communities, intention","PeriodicalId":19330,"journal":{"name":"Nigerian Veterinary Journal","volume":"22 1","pages":"278-286"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87293314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Shinkut, T. Aluwong, P. Rekwot, J. Bugau, F. Samuel, W. O. Echekwu
Agricultural Research Council of Nigeria, Mabushi Abuja; Department of Veterinary Physiology, Ahmadu Bello University, Zaria, Nigeria; National Animal Production Research Institute, Ahmadu Bello University, Zaria, Nigeria; Department of Theriogenology and Production, Faculty of Veterinary Medicine Ahmadu Bello University, Zaria. Nigeria; Biotechnology Research Programme, National Animal Production Research Institute, Ahmadu Bello University, Zaria. Nigeria; Department of Theriogenology and Production, Faculty of Veterinary Medicine, University of Jos, Nigeria.
{"title":"Melatonin and Allium Sativum (Garlic) Protect Dibutyl Phthalate Influence on Spermiogram of Rabbit Bucks","authors":"M. Shinkut, T. Aluwong, P. Rekwot, J. Bugau, F. Samuel, W. O. Echekwu","doi":"10.4314/NVJ.V41I1.7","DOIUrl":"https://doi.org/10.4314/NVJ.V41I1.7","url":null,"abstract":"Agricultural Research Council of Nigeria, Mabushi Abuja; Department of Veterinary Physiology, Ahmadu Bello University, Zaria, Nigeria; National Animal Production Research Institute, Ahmadu Bello University, Zaria, Nigeria; Department of Theriogenology and Production, Faculty of Veterinary Medicine Ahmadu Bello University, Zaria. Nigeria; Biotechnology Research Programme, National Animal Production Research Institute, Ahmadu Bello University, Zaria. Nigeria; Department of Theriogenology and Production, Faculty of Veterinary Medicine, University of Jos, Nigeria.","PeriodicalId":19330,"journal":{"name":"Nigerian Veterinary Journal","volume":"76 1","pages":"47-61"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82012066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Mustapha, Y. Baba, M. Lawal, M. Abdurrahman, E. G. Emmanuel, M. T. Salawudeen, A. D. Ishamsudeen, S. Ubah
Department of Theriogenology and Production, Ahmadu Bello University, Zaria.Department of Veterinary Medicine, University of Ilorin,Ilorin.Department of Veterinary Surgery and Radiology, Ahmadu Bello University Zaria, Nigeria.Veterinary Teaching Hospital, Ahmadu Bello University Zaria, Nigeria.Department of Veterinary Microbiology, Ahmadu Bello University, Zaria, Nigeria. Department of Theriogenology, University of Abuja, Abuja, Nigeria. *Corresponding email: rhyme4bee@yahoo.com; Tel No: +2348035049184
{"title":"Management of Open-Cervix Pyometra in a Four-Year-Old German Shepherd-Cross Bitch","authors":"R. Mustapha, Y. Baba, M. Lawal, M. Abdurrahman, E. G. Emmanuel, M. T. Salawudeen, A. D. Ishamsudeen, S. Ubah","doi":"10.4314/NVJ.V41I1.3","DOIUrl":"https://doi.org/10.4314/NVJ.V41I1.3","url":null,"abstract":"Department of Theriogenology and Production, Ahmadu Bello University, Zaria.Department of Veterinary Medicine, University of Ilorin,Ilorin.Department of Veterinary Surgery and Radiology, Ahmadu Bello University Zaria, Nigeria.Veterinary Teaching Hospital, Ahmadu Bello University Zaria, Nigeria.Department of Veterinary Microbiology, Ahmadu Bello University, Zaria, Nigeria. Department of Theriogenology, University of Abuja, Abuja, Nigeria. *Corresponding email: rhyme4bee@yahoo.com; Tel No: +2348035049184","PeriodicalId":19330,"journal":{"name":"Nigerian Veterinary Journal","volume":"227 1","pages":"18-22"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80156075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dried crayfish is a rich source of protein and an important crustacean consumed all over the world.To determine the occurrence of Salmonella and Shigella in dried crayfish and crayfish sellers practices which may predispose the food item to microbial contamination, dried crayfish were sampled from 100 stalls in Samaru (n=20), Sabo (n=30) and Central market (n=50) markets of Kaduna state. Dried crayfish samples were each homogenized, analyzed for total coliform plate count, then pre-enriched and enriched in peptone water and Rappaport-Vassiliadis broth respectively, which was further cultured on Salmonella Shigella Agar (SSA). Non-lactose fermenting colonies were subjected to biochemical and Sugar tests. The overall mean coliform count was 14.95log10cfu/100ml. Salmonella and Shigella species were isolated from 12 (35.3%) and 5 (14.3%) samples respectively. Locational distribution of dried crayfish showed, Sabon gari (13.3%) and Central market Kaduna (6%) having the highest occurrence of Salmonella and Shigella suspect species respectively. Questionnaire survey administered to 100 of the crayfish sellers revealed that most of them 71 (74%) eat uncooked dried crayfish, 39% (44/100) of them use their bare hands unwashed while packaging the crayfish at point of sale. The presence of Salmonella and Shigella in crayfish from these locations is of public health significance. Therefore, public enlightenment on hygiene, sanitation and proper storage and packaging of crayfish to prevent foodborne disease outbreak such as Salmonellosis and Shigellosis in Nigeria is highly advocated.
{"title":"Occurrence of Salmonella and Shigella on Dried Crayfish (Procambarus Clarkia) Sold in Zaria and Kaduna Central Market, Kaduna State, Nigeria","authors":"G. Kia, S. Mathias, D. Esonu, E. Benjamin","doi":"10.4314/NVJ.V41I1.5","DOIUrl":"https://doi.org/10.4314/NVJ.V41I1.5","url":null,"abstract":"Dried crayfish is a rich source of protein and an important crustacean consumed all over the world.To determine the occurrence of Salmonella and Shigella in dried crayfish and crayfish sellers practices which may predispose the food item to microbial contamination, dried crayfish were sampled from 100 stalls in Samaru (n=20), Sabo (n=30) and Central market (n=50) markets of Kaduna state. Dried crayfish samples were each homogenized, analyzed for total coliform plate count, then pre-enriched and enriched in peptone water and Rappaport-Vassiliadis broth respectively, which was further cultured on Salmonella Shigella Agar (SSA). Non-lactose fermenting colonies were subjected to biochemical and Sugar tests. The overall mean coliform count was 14.95log10cfu/100ml. Salmonella and Shigella species were isolated from 12 (35.3%) and 5 (14.3%) samples respectively. Locational distribution of dried crayfish showed, Sabon gari (13.3%) and Central market Kaduna (6%) having the highest occurrence of Salmonella and Shigella suspect species respectively. Questionnaire survey administered to 100 of the crayfish sellers revealed that most of them 71 (74%) eat uncooked dried crayfish, 39% (44/100) of them use their bare hands unwashed while packaging the crayfish at point of sale. The presence of Salmonella and Shigella in crayfish from these locations is of public health significance. Therefore, public enlightenment on hygiene, sanitation and proper storage and packaging of crayfish to prevent foodborne disease outbreak such as Salmonellosis and Shigellosis in Nigeria is highly advocated.","PeriodicalId":19330,"journal":{"name":"Nigerian Veterinary Journal","volume":"49 1","pages":"32-40"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77834538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tuberculosis (TB) caused by the Mycobacterium tuberculosis complex (MTC) remains a major public health concern due to its high rate of person to person transfer as well as a high level of morbidity and mortality. The risk factors for transmission of zoonotic TB to humans are close physical contact with cattle, consumption of unpasteurised milk and milk products and unhealthy meat processing by butchers are common in developing countries like Nigeria. However, the circulating MTC among the occupationally exposed are unknown therefore the need to determine the prevalence of tuberculosis and to characterize the mycobacterial species in them. A crosssectional study was conducted among butchers, cattle traders and herders in Bodija Municipal Abattoir, Akinyele International Cattle Market and some herds respectively. Using systematic random sampling, 93 sputum samples were collected and analyzed by culture, Mycobacterium Genus Typing as well as Deletion Typing (Multiplex Polymerase Chain Reaction (PCR)). Of the 93 sputa collected, two (2.2%) were positive for mycobacteria by culture which were confirmed to be Mycobacterium africanum by molecular characterization. These bacilli were isolated from two butchers; one of which had the habit of eating raw meat and cherish ‘wara’ (a local soft cheese made from milk). The isolation of M. africanum from butchers in this study raises public health concern on the contamination of the meat processed as well as highlights its importance in the epidemiology of tuberculosis in Nigeria.
{"title":"Isolation and Molecular Characterization of Mycobacterium Africanum from the Sputum of Butchers in a Municipal Abattoir in","authors":"C. A. Agada, I. F. Ijabone, D. Igwe, S. Cadmus","doi":"10.4314/NVJ.V40I4.6","DOIUrl":"https://doi.org/10.4314/NVJ.V40I4.6","url":null,"abstract":"Tuberculosis (TB) caused by the Mycobacterium tuberculosis complex (MTC) remains a major public health concern due to its high rate of person to person transfer as well as a high level of morbidity and mortality. The risk factors for transmission of zoonotic TB to humans are close physical contact with cattle, consumption of unpasteurised milk and milk products and unhealthy meat processing by butchers are common in developing countries like Nigeria. However, the circulating MTC among the occupationally exposed are unknown therefore the need to determine the prevalence of tuberculosis and to characterize the mycobacterial species in them. A crosssectional study was conducted among butchers, cattle traders and herders in Bodija Municipal Abattoir, Akinyele International Cattle Market and some herds respectively. Using systematic random sampling, 93 sputum samples were collected and analyzed by culture, Mycobacterium Genus Typing as well as Deletion Typing (Multiplex Polymerase Chain Reaction (PCR)). Of the 93 sputa collected, two (2.2%) were positive for mycobacteria by culture which were confirmed to be Mycobacterium africanum by molecular characterization. These bacilli were isolated from two butchers; one of which had the habit of eating raw meat and cherish ‘wara’ (a local soft cheese made from milk). The isolation of M. africanum from butchers in this study raises public health concern on the contamination of the meat processed as well as highlights its importance in the epidemiology of tuberculosis in Nigeria.","PeriodicalId":19330,"journal":{"name":"Nigerian Veterinary Journal","volume":"4 1","pages":"306-314"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88589369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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