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Genetic diversity and population structure of tomato brown rugose fruit virus (ToBRFV) variants from Antalya province, Turkey 土耳其安塔利亚省番茄褐皱果病毒(ToBRFV)变异的遗传多样性和群体结构
4区 生物学 Q3 PLANT SCIENCES Pub Date : 2023-09-25 DOI: 10.15835/nbha51313356
Abdullah GÜLLER, Mustafa USTA, Filiz RANDA-ZELYÜT
In this study, bioinformatic analyses were carried out according to the fully coded CP and MP gene regions of the agent, using six novel tomato brown rugose fruit virus (ToBRFV) variants obtained from the production greenhouses in Antalya, where the infection was first detected in Turkey and global variants. Molecular evolutionary analyses using both CP and MP gene regions showed that all variants were distributed in three major clades. Population dynamics studies for both gene regions have shown that there was very low nucleotide diversity and haplotype diversity. The low haplotype diversity for the CP and MP genes indicated almost no recombination status. A strong negative selection was determined for CP and MP gene regions, dN/dS= 0.0877 and dN/dS=0.2104, respectively. Neutrality test results revealed that ToBRFV populations are in an expansion phase. Pairwise comparisons were performed between populations separated in the geographic hierarchy as American, European, and Asian variants, and the findings showed intense gene flow and high genetic similarity (FST<0,33 and migration rate >1). The results of this study reveal the recent population structure of the virus and suggest that necessary precautions should be taken in the international seed trade against contaminated seeds.
在这项研究中,利用从土耳其安塔利亚生产温室获得的6种新型番茄褐褐果病毒(ToBRFV)变体和全球变体,根据该制剂的CP和MP基因全编码区域进行了生物信息学分析。利用CP和MP基因区进行的分子进化分析表明,所有变异分布在三个主要进化支中。种群动态研究表明,这两个基因区域的核苷酸多样性和单倍型多样性都很低。CP和MP基因的低单倍型多样性表明几乎没有重组状态。CP和MP基因区存在较强的负选择,dN/dS分别为0.0877和0.2104。中立性试验结果显示,ToBRFV种群处于扩张阶段。对在地理层次上被划分为美洲、欧洲和亚洲变体的种群进行两两比较,结果显示出强烈的基因流动和高遗传相似性(FST<0,33和迁移率>1)。这项研究的结果揭示了该病毒最近的种群结构,并建议在国际种子贸易中对受污染的种子采取必要的预防措施。
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引用次数: 0
The growth rate and genetic variability of Scots pine (Pinus sylvestris L.) half-sibs in test crops of Northern Kazakhstan 北哈萨克斯坦试验作物中苏格兰松(Pinus sylvestris L.)半兄弟的生长速率和遗传变异
4区 生物学 Q3 PLANT SCIENCES Pub Date : 2023-09-25 DOI: 10.15835/nbha51313261
Yana KREKOVA, Nadezhda CHEBOTKO, Dmitry KAGAN, Svetlana IVANOVSKAYA, Yekaterina VIBE, Andrey KABANOV
Scots pine (Pinus sylvestris L.) is a valuable tree species, playing an important role in maintaining the ecological and economic balance not only in Kazakhstan, but also in many countries around the world. The purpose of this study was to assess the growth rate and to identify the genotypes of the half-sib progeny of Scotch pine plus trees in order to further obtain high-quality seeds with valuable genetic traits. A molecular genetic analysis was carried out using six microsatellite and 20 isoenzyme loci on 15 Scots pine half-sib families, represented by two samples of seeds collected in the subsequent harvesting periods (planted in 1986 and 1987), that evaluated the taxonomic characteristics such as tree height and trunk diameter (DBH, measured at 1.3 meters). Families with the highest growth rate in both samples were selected as candidates for the elite group. During the SSR analysis, a decrease in the level of expected heterozygosity (0.2377-0.5362 with an average value of 0.4593) was observed in P. sylvestris L. half-sibs compared to the sample of maternal plus trees (0.5444), with similar results to that of the “founder effect”. At the same time, the level of observed heterozygosity remained sufficiently high (0.3333-0.7037 with an average value of 0.4980 and 0.4556, respectively). During the isoenzyme analysis, the level of genetic variability either decreased or was similar to that of the maternal trees. Rare and unique alleles were found in seed samples of the analysed tree families. In general, this research showed that a comprehensive evaluation combining both traditional breeding methods (genetic assessment of progeny in the course of growth) and molecular genetic analysis is required for the selection of elite trees.
苏格兰松(Pinus sylvestris L.)是一种珍贵的树种,在维护哈萨克斯坦乃至世界许多国家的生态和经济平衡中发挥着重要作用。本研究的目的是评估苏格兰松加树半同胞后代的生长速率和基因型,以进一步获得具有有价值遗传性状的优质种子。利用6个微卫星和20个同工酶位点对15个苏格兰松半同胞科进行了分子遗传分析,以随后收获期(1986年和1987年种植)收集的2个种子样本为代表,评估了树高和树干直径(DBH,测量值为1.3米)等分类特征。两个样本中增长率最高的家庭被选为精英群体的候选人。SSR分析结果表明,与母系正树(0.5444)相比,柽柳半姐妹的期望杂合度(0.2377 ~ 0.5362,平均值为0.4593)明显降低,与“founder效应”的结果相似。同时,观察到的杂合度保持了足够高的水平(0.3333-0.7037,平均值分别为0.4980和0.4556)。在同工酶分析中,遗传变异水平或降低或与母树相似。在所分析的树科种子样本中发现了罕见和独特的等位基因。总体而言,本研究表明,选择优良树种需要结合传统育种方法(生长过程中后代遗传评价)和分子遗传分析相结合的综合评价。
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引用次数: 0
Biochemical responses of Silybum marianum seedlings to independent and combined salt and SiO2 treatments 水飞蓟幼苗对盐和SiO2单独及复合处理的生化响应
4区 生物学 Q3 PLANT SCIENCES Pub Date : 2023-09-25 DOI: 10.15835/nbha51313375
Lăcrămioara OPRICĂ, Marius-Nicușor GRIGORE, Gabriela VOCHIȚA, Roxana JIJIE, Lucia-Florina POPOVICI
In recent years, silicon dioxide (SiO2) particles have drawn increasing attention due to their potential to enhance plant tolerance to various stresses by modulating physiological and biochemical processes. Salinity is an important abiotic stress with a harmful impact on crop growth and productivity. In this context, the aim of this study was to investigate the influence of silicon dioxide (SiO2) addition on milk thistle (Silybum marianum (L.) Gaertn.) under salinity stress. Thus, individual and combined effects of the application of sodium chloride (NaCl, 50 and 100 mM) and SiO2 particles (0.1 mM) on some physiological and oxidative stress parameters, such as growth variables, photosynthetic pigments and soluble protein contents, the levels of an oxidative stress biomarker (malondialdehyde), and the specific activity of several antioxidant enzymes (superoxide dismutase, catalase and peroxidase) were evaluated. The results revealed that salt stress (100 mM) inhibited plant growth and decreased chlorophyll and carotenoid levels, whereas the added SiO2 partially mitigated these negative effects. In addition, the 100 mM NaCl treatment significantly increased peroxidise (POD) activity and decreased soluble protein content after 22 days, while no significant differences were found for group subjected to salt stress in the presence of SiO2. Results suggest that application of SiO2 on salt-stressed milk thistle may modify the seedling metabolism and increase the plant salt tolerance capacity.
近年来,二氧化硅(SiO2)颗粒因其通过调节生理生化过程提高植物对各种胁迫的耐受性而受到越来越多的关注。盐胁迫是一种重要的非生物胁迫,对作物的生长和生产力产生不利影响。在此背景下,本研究的目的是研究二氧化硅(SiO2)的添加对水飞蓟(Silybum marianum (L.))的影响。在盐度胁迫下。因此,本研究评估了氯化钠(NaCl, 50和100 mM)和二氧化硅(0.1 mM)单独或联合施用对一些生理和氧化应激参数的影响,如生长变量、光合色素和可溶性蛋白含量、氧化应激生物标志物(丙二醛)水平以及几种抗氧化酶(超氧化物歧化酶、过氧化氢酶和过氧化物酶)的比活性。结果表明,盐胁迫(100 mM)抑制了植物生长,降低了叶绿素和类胡萝卜素水平,而添加SiO2可部分缓解这些负面影响。此外,100 mM NaCl处理22 d后显著提高了过氧化物酶(POD)活性,降低了可溶性蛋白含量,而在SiO2存在下盐胁迫组间差异不显著。结果表明,在盐胁迫下施用SiO2可改变水飞蓟幼苗代谢,提高植株耐盐能力。
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引用次数: 0
Application of Rhizopus microsporus and Aspergillus oryzae to enhance the defense capacity of eggplant seedlings against Meloidogyne incognita 应用小孢子根霉和米曲霉提高茄子幼苗对黑线蛾的防御能力
4区 生物学 Q3 PLANT SCIENCES Pub Date : 2023-09-25 DOI: 10.15835/nbha51313300
Mohamed S. ATTIA, Mohamed H. SHARAF, Amr H. HASHEM, Amira Y. MAHFOUZ, Ghadir E. DAIGHAM, Abdulaziz A. AL-ASKAR, Hamada ABDELGAWAD, Mahmoud S. OMAR, Ali E. THABET, Mahmoud M. ABDALMOHSEN, Yousef R. ELADLY, Amer M. ABDELAZIZ
Several phytopathogens attack eggplant, causing crop damage. One of the most destructive plant diseases, Root-Knot Nematode (RKN), causes significant damage to eggplant seedlings. Finding safe and effective biological alternatives to prevent eggplant root nematode disease, which significantly limits plant productivity, is the innovative aspect of this study. Six isolates of plant growth-promoting fungus (PGPF) were tested in the current work for improving biochemical defense and physio-biochemical performance in eggplant seedlings under the Meloidogyne incognita challenge. PGPF isolates were tested in vitro for some biochemical traits such as Siderophores and HCN production. Besides, the antagonistic efficacy of PGPF filtrates against M. incognita was tested in vitro. The best isolates capable of producing HCN were F5 and F3 respectively. Also, F5 followed by F3 exhibited the maximum mortality proportions of 74.20% and 60.35% mortality in nematode juveniles after 96 hours respectively. Moreover, F5 has the highest level of antioxidant activity, with IC50 145 µg/mL followed by F3 with IC50 350 µg/mL. thus, we identified F5 and F3 completely as Rhizopus microsporus (OQ291571.1 and Aspergillus oryzae OQ291572.1. Implementing R. microsporus and A. oryzae collectively in vivo study was the most successful therapy, limiting nematode recordings as 95.23%, 86.98%, 80.35%, 80%, and 68.78% reduction in females, galls, developmental stage, egg masses, second juveniles, respectively, in diseased seedlings. It could be suggested that the use of ethyl acetate extracts (EAE) of A. oryzae and R. microsporus might be commercially applied as a stimulator of eggplant and or anti-nematodes against M. incognita.
几种植物病原体侵袭茄子,造成作物损害。根结线虫(Root-Knot Nematode, RKN)是最具破坏性的植物病害之一,对茄子幼苗造成严重危害。寻找安全有效的生物替代品来预防严重限制植物生产力的茄子根线虫病,是本研究的创新之处。本研究对6株植物生长促进真菌(PGPF)进行了研究,以提高茄子幼苗在未知旋律线虫(Meloidogyne incognita)侵染下的生化防御和生理生化性能。对PGPF分离株进行了体外生化特性测试,如铁载体和HCN产量。此外,还对PGPF滤液进行了体外拮抗实验。产HCN的最佳菌株分别为F5和F3。96 h后,F5和F3的线虫幼虫死亡率最高,分别为74.20%和60.35%。此外,F5的抗氧化活性最高,IC50为145µg/mL,其次是F3, IC50为350µg/mL。因此,F5和F3完全鉴定为小孢子根霉(OQ291571.1)和米曲霉(OQ291572.1)。将小孢子丝虫和米芽孢霉共同进行体内研究是最成功的治疗方法,在患病幼苗中,雌虫、虫瘿、发育阶段、卵块、二幼虫的线虫记录分别减少95.23%、86.98%、80.35%、80%和68.78%。综上所述,利用米芽孢霉和小孢子霉的乙酸乙酯提取物(EAE)可作为茄子的刺激剂和抗线虫剂,用于茄子对隐芽孢霉的拮抗。
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引用次数: 1
Morphological and biochemical changes with hormone and hydro- priming applications in safflower (Carthamus tinctorius L.) seedlings under salinity stress conditions 盐胁迫条件下红花(Carthamus tinctorius L.)幼苗形态和生化变化与激素和水诱导的关系
4区 生物学 Q3 PLANT SCIENCES Pub Date : 2023-09-25 DOI: 10.15835/nbha51313282
Mehtap GÜRSOY
In this study, the ameliorative effects of hydro- and hormopriming applications against salt stress of safflower seeds during germination and seedling development stage were investigated. Primed (hydropriming (0, 1, 2 and 3 days) with distilled water and hormopriming for 24 hours (0, 50, 100 and 150 mg L-1 with kinetin) and nonprimed seeds were sown under control (no salt) and salt stress (0, 50, 100 and 150 mM NaCl) conditions. When priming applications under salt stress are compared with the control, germination percentage (GP), seedling lenght (SL), root length (RL), seedling fresh weight (SFW), root fresh weight (RFW), leaf relative water content (RWC), elctrolyte leakeage (EL) parameters reducing the effects of stress, however, it was determined that caused an increase in carotenoid (Car), superoxide dismutase (SOD), total phenolic compounds (TPC) parameters. Besides this, it has an inhibitory effect on the increase in proline (Pro) and malondialdehyde (MDA) content. According to the correlation analysis, significant positive correlation was determined in all parameters. A significant positive correlation was determined for hydropriming GP with SL, RL, SFW, RFW, and for hormopriming with SL, RL, SFW, RFW, total Chl, RWC and GP. According to the PCA analysis, the parameters examined in both applications it is seen that they are divided into four different groups. In conclusion, this study priming applications are compared with each other, it has been determined that hormopriming is more effective in reducing the effects of stress than hydropriming.
本研究以红花种子萌发期和幼苗发育期为研究目标,研究了水激和激素激对盐胁迫的改善作用。在对照(无盐)和盐胁迫(0、50、100和150 mg L-1)条件下,分别用蒸馏水进行水浸(0、1、2和3天)和用激动素进行激素浸24小时(0、50、100和150 mg L-1)和不进行水浸的种子播种。与对照对照比较,盐胁迫下的引施量对发芽率(GP)、苗长(SL)、根长(RL)、幼苗鲜重(SFW)、根鲜重(RFW)、叶片相对含水量(RWC)、电解质渗漏(EL)等参数的影响减小,但确定盐胁迫导致类胡萝卜素(Car)、超氧化物歧化酶(SOD)、总酚类化合物(TPC)等参数升高。此外,对脯氨酸(Pro)和丙二醛(MDA)含量的增加有抑制作用。经相关分析,各参数均呈显著正相关。水浸GP与SL、RL、SFW、RFW呈显著正相关,激素激发GP与SL、RL、SFW、RFW、总Chl、RWC和GP呈显著正相关。根据PCA分析,在两个应用程序中检查的参数可以看出,它们被分为四个不同的组。综上所述,本研究对不同的启动应用进行了比较,确定了激素启动比氢启动更能有效地减少应激的影响。
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引用次数: 0
Synergism of microorganisms and seaweed extract on vegetative growth, yield and quality of cucumber fruit 微生物和海藻提取物对黄瓜果实营养生长、产量和品质的增效作用
4区 生物学 Q3 PLANT SCIENCES Pub Date : 2023-09-25 DOI: 10.15835/nbha51312888
Alonso MENDEZ, Silvia MARTINEZ, Aida LEAL, Armando HERNANDEZ, Juana GARCÍA, Miriam SÁNCHEZ
Natural biostimulants, such as microorganisms and seaweed extracts, are used in agriculture to improve crop yields with a sustainable approach. However, the interaction effects between different biostimulants have not been thoroughly investigated. The objective of this work was to evaluate the synergistic effects of microorganisms (Glomus intraradices and Azospirillum brasilense) and seaweed extract (Ascophyllum nodosum and Macrocytis pyrifera) on growth, yield and fruit quality of cucumber plants under soilless cultivation. Two doses of microorganisms (0 and 10 spores mL-1 + 106 CFU) and four concentrations of seaweed extract (0, 250, 500 and 2500 ppm) were evaluated. The experimental design used was a randomized complete block design with a factorial arrangement (2 x 4), with four replications per treatment. The results showed that the equatorial diameter of the fruit, yield and titratable acidity improve when applying microorganisms and seaweed extract in combination; however, when applying the two biostimulants the values ​​of plant height, stem diameter, number of leaves, root length, biomass (fresh and dry), firmness, total soluble solids, vitamin C, chlorophyll (a and total) and indices of color (L* and b*) did not exceed those obtained when the biostimulants were applied individually. The combined application of microorganisms and seaweed extract improves cucumber yield, but not vegetative growth and, except for titratable acidity, fruit quality attributes.
天然生物刺激剂,如微生物和海藻提取物,被用于农业,以可持续的方式提高作物产量。然而,不同生物刺激素之间的相互作用尚未得到充分的研究。本研究旨在探讨微生物(Glomus intraradices和Azospirillum brasilense)和海藻提取物(Ascophyllum nodosum和Macrocytis pyrifera)对无土栽培黄瓜生长、产量和果实品质的协同效应。评估了两种剂量的微生物(0和10孢子mL-1 + 106 CFU)和四种浓度的海藻提取物(0、250、500和2500 ppm)。实验设计采用随机完全区组设计,阶乘排列(2 x 4),每个治疗4个重复。结果表明:微生物与海藻浸出液配合施用可提高果实赤道径、产量和可滴定酸度;然而,当施用两种生物刺激剂时,株高、茎粗、叶数、根长、生物量(新鲜和干燥)、硬度、总可溶性固形物、维生素C、叶绿素(a和total)和颜色指数(L*和b*)的值均不超过单独施用时的值。微生物和海藻提取物的联合施用提高了黄瓜产量,但对营养生长没有影响,除了可滴定的酸度外,对果实品质也没有影响。
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引用次数: 0
Chemical composition of Tithonia diversifolia (Hemsl.) and its effect on growth performance, feed efficiency and metabolic biochemistry of juvenile hybrid tilapia Oreochromis mossambicus × Oreochromis niloticus 多叶罗非鱼(Tithonia varfolia, Hemsl.)的化学成分及其对mossambicus × nilochromis杂交罗非鱼幼鱼生长性能、饲料效率和代谢生化的影响
4区 生物学 Q3 PLANT SCIENCES Pub Date : 2023-09-21 DOI: 10.15835/nbha51313337
Yuniel MÉNDEZ-MARTÍNEZ, Rosa I. NARVÁEZ-NARVÁEZ, Carlos ANGULO, Edilmar CORTÉS-JACINTO, Aroldo BOTELLO-LEON, Danis M. VERDECIA-ACOSTA, Yenny G. TORRES-NAVARRETE
The aquaculture due to the high cost and continued shortage of animal protein sources, the development of alternative plant protein sources has been one of the main challenges to establish sustainable aquaculture as economically viable. Objective of this investigation was to determine the chemical composition and effect of Tithonia diversifolia on the growth performance, feed efficiency and metabolic biochemistry of juveniles of Oreochromis mossambicus × Oreochromis niloticus. Five treatments were used: 0% (control), 4, 8, 12 and 16% levels of inclusion of T. diversifolia flour in diet to feed juvenile fish cultured in 15 plastic aquariums at a density of 15 fish/aquarium. The assay lasted eight weeks. The T. diversifolia flour was characterized for showing adequate levels of crude protein (21%), with low crude fat (4.5%) levels, neutral detergent fiber levels: 43.03% and acid detergent fiber: 24.40%. The growth performance presented significant differences (p˂0.05), for weight gain, day average weight gain (DAW), length gain, day average length gain (DAL), specific growth rate (SGR), feed efficiency (FE), protein efficiency ratio (PER) and feed conversion ratio (FCR). In treatment with 16% of inclusion of T. diversifolia flour, values obtained for weight gain, DAW, SGR, FE and PER (15.88 g, 0.28 g. day-1, 2.03 %, 0.34 g.g-1 and 0.98, respectively), which did not differ with the control treatment, highlighting the benefits of T diversifolia flour. The metabolic biochemistry parameters experienced a decrease with the increase of T. diversifolia in diet, where the highest values of triglycerides, cholesterol, and glucose were for the control treatment (p <0.05), while the protein increased to 4% of the flour. T. diversifolia flour presents an adequate balance of nutrients for its chemical composition and amino acids, without causing negative effects on the growth performance, weight-length relationship, feed efficiency, survival and metabolic biochemistry.
由于水产养殖的高成本和动物蛋白来源的持续短缺,开发替代植物蛋白来源一直是建立可持续水产养殖的主要挑战之一。本试验的目的是研究黄颡鱼(Tithonia varfolia)的化学成分及其对莫沙×尼罗鱼(Oreochromis mossambicus × nilochromis)幼鱼生长性能、饲料效率和代谢生化的影响。采用0(对照)、4、8、12和16%的饲料添加量,饲喂15个塑料水族箱养殖的幼鱼,密度为15只/个。实验持续8周。该粉粗蛋白质含量充足(21%),粗脂肪含量低(4.5%),中性洗涤纤维含量为43.03%,酸性洗涤纤维含量为24.40%。在增重、日平均增重(DAW)、体长增重(DAL)、特定生长率(SGR)、饲料效率(FE)、蛋白质效率(PER)和饲料系数(FCR)方面差异显著(p小于0.05)。添加16%黄粉处理的增重、DAW、SGR、FE和PER值(分别为15.88 g、0.28 g. day-1、2.03%、0.34 g. day-1和0.98)与对照处理无显著差异,表明黄粉处理具有显著的增效作用。代谢生化指标随黄颡鱼粉添加量的增加而降低,其中甘油三酯、胆固醇和葡萄糖含量以对照处理最高(p <0.05),而蛋白质含量则增加到面粉的4%。大黄粉在化学成分和氨基酸方面营养均衡,对生长性能、体长关系、饲料效率、成活率和代谢生化没有负面影响。
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引用次数: 1
Metabolome and transcriptome analyses reveal the colouring mechanism of red honeysuckle (Lonicera japonica Thunb.) 代谢组学和转录组学分析揭示了红金银花(Lonicera japonica Thunb)的着色机制。
4区 生物学 Q3 PLANT SCIENCES Pub Date : 2023-09-21 DOI: 10.15835/nbha51313150
Xiaodong ZHANG, Caixia LI, Zhanchao HAO
Honeysuckle has been widely used as a medicinal herb and food additive in China for a long time. However, little is known about the pigment composition and colouring mechanism of red honeysuckle, which is a rare germplasm resource. This study aims to investigate the anthocyanin components and colouring mechanism of red honeysuckle, and to identify potential regulatory genes in the anthocyanin biosynthesis pathway. ‘Yujin 1’ and ‘Yujin 2’, with yellow-white and red flower buds, respectively, were selected for the study. Using a metabolomics method, we identified the anthocyanin components, while transcriptomics analysis was used to mine the structural and regulatory genes of the anthocyanin biosynthesis pathway. Additionally, protein-protein interaction analysis was employed to predict the regulation mechanism of anthocyanin biosynthesis. The results revealed that cyanidin-3,5-O-diglucoside, peonidin-3,5-O-diglucoside, and cyanidin-3-O-glucoside were the main pigment components of red honeysuckle. We also constructed a possible anthocyanin biosynthetic pathway and identified MYB and bHLH transcription factors that may play regulatory roles in this pathway. Furthermore, our findings suggest that bHLH23 may regulate anthocyanin biosynthesis by binding to the DFR gene promoter. These findings have significant implications for breeding new honeysuckle varieties and developing functional foods and medicines.
金银花作为一种中草药和食品添加剂在中国被广泛使用已久。然而,红金银花作为一种罕见的种质资源,其色素组成和着色机理尚不清楚。本研究旨在研究红金银花花青素的成分和著色机制,并鉴定花青素生物合成途径中潜在的调控基因。选择花蕾分别为黄白色和红色的“玉金1号”和“玉金2号”进行研究。利用代谢组学方法,我们鉴定了花青素成分,而转录组学分析用于挖掘花青素生物合成途径的结构和调控基因。此外,利用蛋白-蛋白相互作用分析预测了花青素生物合成的调控机制。结果表明,花青素-3,5- o -二葡萄糖苷、芍药苷-3,5- o -二葡萄糖苷和花青素-3- o -葡萄糖苷是红金银花色素的主要成分。我们还构建了一个可能的花青素生物合成途径,并鉴定了MYB和bHLH转录因子可能在该途径中发挥调控作用。此外,我们的研究结果表明bHLH23可能通过结合DFR基因启动子来调节花青素的生物合成。这些发现对培育金银花新品种、开发功能性食品和药物具有重要意义。
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引用次数: 0
Micropropagation of autochthonous olive varieties from Türkiye 台湾橄榄本地品种的微繁研究
4区 生物学 Q3 PLANT SCIENCES Pub Date : 2023-09-21 DOI: 10.15835/nbha51313267
Zeliha CIFTCI, Ebru SAKAR, Sezai ERCISLI, Mehmet Ilhan ODABASIOGLU, Dejan ZEJAK, Velibor SPALEVIC
Olive (Olea europaea L.) is one of the oldest cultivated fruit species in the world. Fruits and oils of autocthonous olive varieties (native Turkish olive varieties) with unique sensory properties (taste, smell and aroma) gain importance recently. Particularly olive oil companies looking for varieties that have distinct taste, smell and aroma in their oil. Propagation of olive varieties by cuttings and grafting is very difficult and expensive, therefore it is important to find solution for easy and mass propagation. Micropropagation is particularly beneficial to propagate plants that are difficult to reproduce conventionally or to ensure virus-free plants or plants with particular qualities. In this study, in vitro micropropagation success of two autocthonous olive varieties (‘Mavi’ and ‘Guleki’) grown in the origin center of olive, Southeast Anatolia, Türkiye was investigated. Both varieties, have distinct smell, taste and aroma and are difficult to root by cuttings. The effects of three different medium OM (Olive Medium), WPM (Woody Plant Medium) and DKW (Driver-Kuniyuki Walnut Medium) and two different growth regulators BAP (6-Benzylaminopurine) and Zeatin on shoot induction (proliferation) in the in vitro micropropagation of the olive varieties were examined. Obtained shoots were later subjected to in vitro rooting and acclimatization. The highest proliferation efficiency and shoot length for both varieties were obtained with the use of 1 mg Zeatin+0.1 mg GAɜ hormone combinations on OM medium. ‘Mavi’ variety formed more roots compared to ‘Guleki’ (3.33 vs. 2.75 per shoots), and gave the highest rooting rate of 74.33% with 2 mg IBA (Indole Butyric Acid) treatment on ½ OM medium. In terms of rooting rate, Guleki gave the highest rooting (100%) on medium containing ½ OM, 0.2 mg GA3 and 4 mg IBA. 60% and 75% of the micropropagated plants of ‘Mavi’ and ‘Guleki’ varieties adapted well to the external conditions.
橄榄(Olea europaea L.)是世界上最古老的栽培水果之一。土生橄榄品种(土生土耳其橄榄品种)的果实和油具有独特的感官特性(味觉、嗅觉和香气),近年来越来越受到重视。尤其是橄榄油公司,他们在寻找那些在油中有独特味道、气味和香气的品种。橄榄品种的扦插和嫁接繁殖是非常困难和昂贵的,因此寻找容易和大量繁殖的解决方案是很重要的。微繁繁殖特别有利于繁殖常规繁殖难以繁殖的植物或确保无病毒植物或具有特殊品质的植物。本研究以安那托利亚东南部橄榄产地 rkiye为研究对象,对两个本地橄榄品种(‘Mavi’和‘Guleki’)的离体微繁效果进行了研究。这两个品种,有独特的气味,味道和香气,很难通过扦插生根。研究了3种不同培养基OM(橄榄培养基)、WPM(木本植物培养基)和DKW(驱动-国之核桃培养基)以及2种不同生长调节剂BAP(6-苯氨基嘌呤)和玉米素对橄榄品种离体微增殖诱导(增殖)的影响。获得的芽随后进行离体生根和驯化。在OM培养基上,1 mg玉米素+0.1 mg GA / a激素组合的增殖效率和芽长均最高。与“Guleki”相比,“Mavi”品种的生根数量更多(3.33根比2.75根),在½OM培养基上处理2 mg IBA(吲哚丁酸)时,生根率最高,为74.33%。在含1 / 2 OM、0.2 mg GA3和4 mg IBA的培养基上生根率最高,达100%。“Mavi”和“Guleki”品种的60%和75%的小苗对外部条件适应良好。
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引用次数: 0
Ecotype effects on photosynthesis performance using A/PFFD among Pinus nigra Arn. A/PFFD对黑松光合性能的生态型影响
4区 生物学 Q3 PLANT SCIENCES Pub Date : 2023-09-15 DOI: 10.15835/nbha51312599
Sondes Fkiri, Touhami Rzigui, Hanene Ghazghazi, Larbi M. Khouja, Abelhamid Khaldi, Frédéric Guibal, Zouheir Nasr
This study aimed to provide insights on intraspecific variability of photosynthesis performance of 19 provenances of black pine planted in a common garden. The experiment was conducted in an experimental trial located at Souiniet (the southern limit of its range) Photosynthetic capacity char­acterized by light-saturated net photosynthetic rates, associated light compensation points and apparent quantum yield was monitored by measuring the response of leaf gas exchange to light levels. Amax was the highest in provenances Puget Theniers (nigra ssp), Saint-Guilhem (salzmanni ssp), Marghese and les barres (laricio ssp). The lowest value was recorded in Olette of the subspecies salzmanni. Needles from two provenances Crimea (pallasiana ssp) and Les Barres (ssp laricio) revealed the highest apparent quantum yield (ɸ), followed by Brougatlès Ales (salzmanni ssp), Trenta Coste (ssp laricio), Les barres and Puget Theniers (nigra ssp). The lowest apparent quantum Yield was recorded in Laricio subspecies (Cosenza; Les barres and Bois frerot). The highest value of dark respiration (Rd) was shown in provenance Les barres (laricio ssp), while Grancia and Tavola of the laricio subspecies reported the lowest one. Provenances Tavola (laricio ssp) and Puget Theniers (nigra ssp) exhibited the highest LCP. The provenance Crimée (pallasiana ssp) and Aspromonto (laricio ssp) recorded the lowest values. The maximum values of photosynthesis are positively correlated with the total chlorophyll contents and Leaf Mass area. Our study illustrates that Photosynthesis performance showed a difference between 19 provenances of black pine; it seems that black pine photosynthetic performance is eco-typical independent.
本研究的目的是了解普通园林19个种源黑松光合性能的种内变异。通过测量叶片气体交换对光照水平的响应,监测以光饱和净光合速率、相关光补偿点和表观量子产率为表征的光合能力。Amax在普吉特特尼尔斯(nigra ssp)、圣吉尔海姆(salzmanni ssp)、马格塞(Marghese)和莱斯巴雷斯(laricio ssp)种源中最高。最小值记录在salzmanni亚种的Olette。两个种源克里米亚(pallasiana ssp)和Les Barres (ssp laricio)的表观量子产率最高,其次是brougatl Ales (salzmanni ssp)、Trenta Coste (ssp laricio)、Les Barres和Puget Theniers (nigra ssp)。Laricio亚种(Cosenza;Les barres和Bois frerot)。暗呼吸(Rd)值以种源Les barres (laricio ssp)最高,落叶松亚种Grancia和Tavola最低。种源Tavola (laricio ssp)和Puget Theniers (nigra ssp)的LCP最高。种源crimone (pallasiana ssp)和Aspromonto (laricio ssp)的值最低。光合作用最大值与总叶绿素含量和叶质量面积呈正相关。研究表明,19个种源间黑松光合性能存在差异;似乎黑松的光合性能是生态典型独立的。
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Notulae Botanicae Horti Agrobotanici Cluj-napoca
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