Telosma cordata has long been consumed as foods and herbal medicine. The aim of this research is to investigate the potential biological activities of Telosma cordata flower (TCF) extracts and their molecular mechanisms in the treatment of neurological diseases, especially dementia. In general, the plant samples were successfully identified through DNA barcoding regions, utilizing matK, trnH-psbA, and rbcL markers. The ethanol extract and its fraction exhibited significantly high acetylcholinesterase inhibitory activity. Moreover, hexane extract of T. cordata displayed the most potent neurotrophic activity in a preliminary cell-based screening based on C6 cells neurite outgrowth. Additionally, hexane extract of T. cordata demonstrated the highest antioxidant activity. Finally, the hexane extract of T. cordata upregulated the expression of BDNF, NGF and acetyl H3 in C6 cells, affecting both mRNA and protein levels. These findings indicate that Telosma cordata could be a strong candidate for developing pharmacological drugs to treat dementia and neurodegenerative diseases.
{"title":"Hexane extract of Telosma cordata enhances neurite outgrowth via the epigenetically regulated genes expression in neuronal cells","authors":"Nguyet Thi DOAN, Hung Minh NGUYEN, Tuyet Loan Thi BUI, Huyen Trang Thi NGUYEN, Duong Hoang Nguyen, Truyen van Nguyen","doi":"10.15835/nbha51413204","DOIUrl":"https://doi.org/10.15835/nbha51413204","url":null,"abstract":"Telosma cordata has long been consumed as foods and herbal medicine. The aim of this research is to investigate the potential biological activities of Telosma cordata flower (TCF) extracts and their molecular mechanisms in the treatment of neurological diseases, especially dementia. In general, the plant samples were successfully identified through DNA barcoding regions, utilizing matK, trnH-psbA, and rbcL markers. The ethanol extract and its fraction exhibited significantly high acetylcholinesterase inhibitory activity. Moreover, hexane extract of T. cordata displayed the most potent neurotrophic activity in a preliminary cell-based screening based on C6 cells neurite outgrowth. Additionally, hexane extract of T. cordata demonstrated the highest antioxidant activity. Finally, the hexane extract of T. cordata upregulated the expression of BDNF, NGF and acetyl H3 in C6 cells, affecting both mRNA and protein levels. These findings indicate that Telosma cordata could be a strong candidate for developing pharmacological drugs to treat dementia and neurodegenerative diseases.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"34 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139273652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The papers published in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Issue 3, Volume 51, 2023 represent new exciting research in different topics of life science, respectively in plant science, horticulture, agronomy, crop science, and forestry. Among the interesting articles we invite you to find news about: Comparison of the potential of extracts from the flower, fruit pulp, and seed of Cassia fistula L. on MCF-7 breast cancer cell growth and cell migration; Evaluation of anticancer activity of Plumbago indica root extract on cervical HeLa cancer cells; Metabolome and transcriptome analyses reveal the colouring mechanism of red honeysuckle (Lonicera japonica Thunb.); Ethylene modulates root growth and mineral nutrients levels in trifoliate orange through the auxin-signaling pathway; Green nanotechnology for plant bacterial diseases management in cereal crops: a review on metal-based nanoparticles; Physiological responses of coffee (Coffea arabica L.) plants to biochar application under water deficit conditions; Gene mining and functional analysis related to maize (Zea mays L.) seed size; Phytochemical properties, antioxidant potential and fatty acids profiling of three dragon fruit species grown under sub-tropical climate; Chemical composition and anti-inflammatory effect of Phellodendron amurense Rupr. stem bark extract; Ecotype effects on photosynthesis performance using A/PFFD among Pinus nigra Arn.; Assessment of herbaceous vegetation classification using orthophotos produced from the image acquired with unmanned aerial systems.
{"title":"Introduction pages, NBHA-CN 51(3), 2023","authors":"Radu E. SESTRAS","doi":"10.15835/nbha51313432","DOIUrl":"https://doi.org/10.15835/nbha51313432","url":null,"abstract":"The papers published in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Issue 3, Volume 51, 2023 represent new exciting research in different topics of life science, respectively in plant science, horticulture, agronomy, crop science, and forestry. Among the interesting articles we invite you to find news about: Comparison of the potential of extracts from the flower, fruit pulp, and seed of Cassia fistula L. on MCF-7 breast cancer cell growth and cell migration; Evaluation of anticancer activity of Plumbago indica root extract on cervical HeLa cancer cells; Metabolome and transcriptome analyses reveal the colouring mechanism of red honeysuckle (Lonicera japonica Thunb.); Ethylene modulates root growth and mineral nutrients levels in trifoliate orange through the auxin-signaling pathway; Green nanotechnology for plant bacterial diseases management in cereal crops: a review on metal-based nanoparticles; Physiological responses of coffee (Coffea arabica L.) plants to biochar application under water deficit conditions; Gene mining and functional analysis related to maize (Zea mays L.) seed size; Phytochemical properties, antioxidant potential and fatty acids profiling of three dragon fruit species grown under sub-tropical climate; Chemical composition and anti-inflammatory effect of Phellodendron amurense Rupr. stem bark extract; Ecotype effects on photosynthesis performance using A/PFFD among Pinus nigra Arn.; Assessment of herbaceous vegetation classification using orthophotos produced from the image acquired with unmanned aerial systems.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135246917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mehvish RAMZAN, Wang LIHONG, Huma TARIQ, Syed F.A. GILLANI, Saima SHAFIQUE, Adnan RASHEED, Mahmoud F. SELEIMAN
Wheat (Triticum aestivum L.) is the important and strategic cereal crop for the majority of world’s populations. It is significant staple food of about two billion people. In next few years, world demand for wheat is expected to be 40 percent higher than that of its level today. Keeping in view the importance of the crop research work was conducted in the laboratory of Plant Breeding and Molecular Genetics, University of Poonch Rawalakot, AJK, Pakistan. The aim of the research was to find out the genetic diversity of different wheat lines. In the experiment, 50 different lines of wheat species (Triticum aestivum L.) was used to detect genetic diversity by utilizing Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and biochemical analysis. On the basis of biochemical analysis lines 3111 and 3123 was diverse among 50 lines for antioxidant activity by using DPPH radical and 3135 and 3139 for phenolic contents and for flavonoid 3148 and 3107 was found more promising. Molecular characterization by SDS PAGE showed diversity in three wheat lines 3136, 3138 and 3110. These wheat lines could be our potential lines for future wheat improvement program as they were also promising regarding to the high yields.
小麦(Triticum aestivum L.)是世界上大多数人口重要的战略粮食作物。它是大约20亿人的重要主食。未来几年，世界对小麦的需求预计将比目前的水平高出40%。考虑到作物研究工作的重要性，在巴基斯坦AJK的Poonch Rawalakot大学植物育种和分子遗传学实验室进行。这项研究的目的是找出不同小麦品系的遗传多样性。以小麦(Triticum aestivum L.) 50个不同品系为研究材料，采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳和生化分析技术对其遗传多样性进行了检测。在生化分析的基础上，3111和3123对DPPH自由基的抗氧化能力在50个品系中表现出差异，3135和3139对酚类和类黄酮的抗氧化能力表现出差异，3148和3107表现出较好的抗氧化能力。SDS - PAGE分子鉴定表明，小麦品系3136、3138和3110具有多样性。这些小麦品系也具有高产的潜力，可以作为我们未来小麦改良项目的潜在品系。
{"title":"Assessment of genetic divergence in wheat lines (Triticum aestivum L.) involving biochemical and protein markers in rainfed conditions","authors":"Mehvish RAMZAN, Wang LIHONG, Huma TARIQ, Syed F.A. GILLANI, Saima SHAFIQUE, Adnan RASHEED, Mahmoud F. SELEIMAN","doi":"10.15835/nbha51313313","DOIUrl":"https://doi.org/10.15835/nbha51313313","url":null,"abstract":"Wheat (Triticum aestivum L.) is the important and strategic cereal crop for the majority of world’s populations. It is significant staple food of about two billion people. In next few years, world demand for wheat is expected to be 40 percent higher than that of its level today. Keeping in view the importance of the crop research work was conducted in the laboratory of Plant Breeding and Molecular Genetics, University of Poonch Rawalakot, AJK, Pakistan. The aim of the research was to find out the genetic diversity of different wheat lines. In the experiment, 50 different lines of wheat species (Triticum aestivum L.) was used to detect genetic diversity by utilizing Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and biochemical analysis. On the basis of biochemical analysis lines 3111 and 3123 was diverse among 50 lines for antioxidant activity by using DPPH radical and 3135 and 3139 for phenolic contents and for flavonoid 3148 and 3107 was found more promising. Molecular characterization by SDS PAGE showed diversity in three wheat lines 3136, 3138 and 3110. These wheat lines could be our potential lines for future wheat improvement program as they were also promising regarding to the high yields.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135426018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad T. ASLAM, Imran KHAN, Muhammad U. CHATTHA, Rizwan MAQBOOL, Muhammad ZIAULHAQ, Wang LIHONG, Sajid USMAN, Adnan RASHEED, Muhammad U. HASSAN, Mohamed HASHEM, Rehab O. ELNOUR, Muhammad M. IQBAL, Muhammad ARSHAD
Salinity stress is a serious abiotic stress that negatively affect the crop growth and development. Mineral nutrient supplementation is considered as an effective strategy to mitigate the adverse effects of salinity. Nitrogen (N) is an important nutrient needed for plants and its application also an effective strategy to mitigate adverse impacts of salinity. Salinity stress disturbs plant physiological, and biochemical functions, antioxidant activities, cellular membranes, antioxidant activities and nutrient uptake thereby cause significant reduction in plant growth and development. The application of N maintains membrane stability, plant water relations, leaf gas exchange characteristics, and protect the plants from oxidative damages which induce the salt tolerance in plants. Besides, this N also improves nutrient uptake and it also induce cellular signaling that mitigate the adverse impacts of salinity. Therefore, it is interesting to understand the role of N in inducing salt tolerance in plants. In present review the mechanisms of N uptake and assimilation in plants under saline conditions are discussed. The present review provides information on how N mitigates ionic toxicity, and oxidative damages and maintains nutrient balance to counter the toxic effects of salinity stress in plants. This review will help the readers to learning more about the role of N in inducing salt tolerance in plants.
{"title":"The critical role of nitrogen in plants facing the salinity stress: Review and future prospective","authors":"Muhammad T. ASLAM, Imran KHAN, Muhammad U. CHATTHA, Rizwan MAQBOOL, Muhammad ZIAULHAQ, Wang LIHONG, Sajid USMAN, Adnan RASHEED, Muhammad U. HASSAN, Mohamed HASHEM, Rehab O. ELNOUR, Muhammad M. IQBAL, Muhammad ARSHAD","doi":"10.15835/nbha51313347","DOIUrl":"https://doi.org/10.15835/nbha51313347","url":null,"abstract":"Salinity stress is a serious abiotic stress that negatively affect the crop growth and development. Mineral nutrient supplementation is considered as an effective strategy to mitigate the adverse effects of salinity. Nitrogen (N) is an important nutrient needed for plants and its application also an effective strategy to mitigate adverse impacts of salinity. Salinity stress disturbs plant physiological, and biochemical functions, antioxidant activities, cellular membranes, antioxidant activities and nutrient uptake thereby cause significant reduction in plant growth and development. The application of N maintains membrane stability, plant water relations, leaf gas exchange characteristics, and protect the plants from oxidative damages which induce the salt tolerance in plants. Besides, this N also improves nutrient uptake and it also induce cellular signaling that mitigate the adverse impacts of salinity. Therefore, it is interesting to understand the role of N in inducing salt tolerance in plants. In present review the mechanisms of N uptake and assimilation in plants under saline conditions are discussed. The present review provides information on how N mitigates ionic toxicity, and oxidative damages and maintains nutrient balance to counter the toxic effects of salinity stress in plants. This review will help the readers to learning more about the role of N in inducing salt tolerance in plants.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135426013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Usman SHAFQAT, Awais MAQSOOD, Aneeza ISHFAQ, Sadia MUSTAFA, Yumna RASHEED, Faisal MAHMOOD, Muhammad U. HASSAN, Jameel Mohammed AL-KHAYRI, Mohammed Ibrahim ALDAEJ, Muhammad Naeem SATTAR, Adel Abdel-Sabour REZK, Mustafa Ibrahim ALMAGHASLA, Wael Fathi SHEHATA, Tarek Abdelaziz SHALABY
Cereals are an important source of nutrients for animals. Several diseases cause severe yield loss in cereal crops. Bacterial diseases result in varying yield losses across cereals: Wheat (5-40%), maize (15-98.9%), rice (20-70%), pearl millet (3-35%), and oats (15-49%). Diseases may be bacterial diseases, fungal or viral. Bacterial diseases are traditionally treated by pesticides. Chemically synthesized pesticides are toxic and hazardous to the environment. Nanotechnology is emerging and novel field for agriculture, especially in plant pathology as a strong antimicrobial agent. Nanoparticles have been synthesized in various ways i.e., biological, physical, and chemical methods. Chemical and physical methods of nanoparticles are costly and toxic to the environment. The biological method for the synthesis of nanoparticles is eco-friendly and economical. Microorganisms or plant extracts are used for metal nanoparticle synthesis. The application of nanoparticles in agriculture has a wide scope and it can bring nano-revolution. This review summarizes the antibacterial activity of biosynthesized metal nanoparticles and their role in bacterial disease management of cereals.
{"title":"Green nanotechnology for plant bacterial diseases management in cereal crops: a review on metal-based nanoparticles","authors":"Usman SHAFQAT, Awais MAQSOOD, Aneeza ISHFAQ, Sadia MUSTAFA, Yumna RASHEED, Faisal MAHMOOD, Muhammad U. HASSAN, Jameel Mohammed AL-KHAYRI, Mohammed Ibrahim ALDAEJ, Muhammad Naeem SATTAR, Adel Abdel-Sabour REZK, Mustafa Ibrahim ALMAGHASLA, Wael Fathi SHEHATA, Tarek Abdelaziz SHALABY","doi":"10.15835/nbha51313333","DOIUrl":"https://doi.org/10.15835/nbha51313333","url":null,"abstract":"Cereals are an important source of nutrients for animals. Several diseases cause severe yield loss in cereal crops. Bacterial diseases result in varying yield losses across cereals: Wheat (5-40%), maize (15-98.9%), rice (20-70%), pearl millet (3-35%), and oats (15-49%). Diseases may be bacterial diseases, fungal or viral. Bacterial diseases are traditionally treated by pesticides. Chemically synthesized pesticides are toxic and hazardous to the environment. Nanotechnology is emerging and novel field for agriculture, especially in plant pathology as a strong antimicrobial agent. Nanoparticles have been synthesized in various ways i.e., biological, physical, and chemical methods. Chemical and physical methods of nanoparticles are costly and toxic to the environment. The biological method for the synthesis of nanoparticles is eco-friendly and economical. Microorganisms or plant extracts are used for metal nanoparticle synthesis. The application of nanoparticles in agriculture has a wide scope and it can bring nano-revolution. This review summarizes the antibacterial activity of biosynthesized metal nanoparticles and their role in bacterial disease management of cereals.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135586334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haocheng WANG, Binjuan YANG, Haiying TANG, Athar MAHMOOD, Rizwan MAQBOOL, Muhammad U. HASSAN, Tahir A. KHAN, Muhammad M. IQBAL, Guoqin HUANG
Winter cropping is widely considered as an effective way to increase rice yield. Therefore, this study was performed to find optimal winter cropping patterns in middle and lower reaches of the Yangtze River, China. Five experimental treatments were set up based on the long-term field experiment including winter fallow (CK), Chinese milk vetch winter cropping pattern (CRR), rapeseed winter cropping pattern (RRR), garlic winter cropping pattern (GRR), and winter multiple cropping rotation (ROT). The effects of different winter cropping patterns on the yield, dry matter accumulation and translocation, and plant nitrogen uptake were explored in double-cropping rice. The results showed that compared with CK, winter cropping increased the early and late rice yield by 7.91-10.70% and 3.57-6.89%, respectively. Similarly, compared with CK, winter cropping patterns also increased the number of spikes in early rice by 19.36-25.81% and CRR and ROT increased the effective number of spikes in late rice by 25.58% and 23.26% respectively. The dry matter (DM) translocation by stem and leaf of early rice under CRR was highest, with a 36.2% increase compared with CK, however, DM accumulation after heading in early rice under CRR was lowest. GRR decreased the dry matter translocation by stem and leaf in early rice but increased DM accumulation after heading in early and late rice by 65.28% and 13.44% as compared to CK. Moreover, ROT increased the dry matter translocation by stem and leaf in late rice by 112.63%. Additionally, GRR and ROT treatments increased the stem nitrogen uptake in early rice by 61.76% and 58.61% as compared to control, while CRR increased the nitrogen uptake by stem, leave and spike in late rice by 40.76%, 49.51% and 42.92%. In conclusion, CRR is more beneficial to increase DM accumulation and translocation by stem and leaf in double-cropping rice, and nitrogen uptake by rice plants.
{"title":"Winter cropping improves yield, dry matter accumulation and translocation and nitrogen uptake of double-cropping rice","authors":"Haocheng WANG, Binjuan YANG, Haiying TANG, Athar MAHMOOD, Rizwan MAQBOOL, Muhammad U. HASSAN, Tahir A. KHAN, Muhammad M. IQBAL, Guoqin HUANG","doi":"10.15835/nbha51313299","DOIUrl":"https://doi.org/10.15835/nbha51313299","url":null,"abstract":"Winter cropping is widely considered as an effective way to increase rice yield. Therefore, this study was performed to find optimal winter cropping patterns in middle and lower reaches of the Yangtze River, China. Five experimental treatments were set up based on the long-term field experiment including winter fallow (CK), Chinese milk vetch winter cropping pattern (CRR), rapeseed winter cropping pattern (RRR), garlic winter cropping pattern (GRR), and winter multiple cropping rotation (ROT). The effects of different winter cropping patterns on the yield, dry matter accumulation and translocation, and plant nitrogen uptake were explored in double-cropping rice. The results showed that compared with CK, winter cropping increased the early and late rice yield by 7.91-10.70% and 3.57-6.89%, respectively. Similarly, compared with CK, winter cropping patterns also increased the number of spikes in early rice by 19.36-25.81% and CRR and ROT increased the effective number of spikes in late rice by 25.58% and 23.26% respectively. The dry matter (DM) translocation by stem and leaf of early rice under CRR was highest, with a 36.2% increase compared with CK, however, DM accumulation after heading in early rice under CRR was lowest. GRR decreased the dry matter translocation by stem and leaf in early rice but increased DM accumulation after heading in early and late rice by 65.28% and 13.44% as compared to CK. Moreover, ROT increased the dry matter translocation by stem and leaf in late rice by 112.63%. Additionally, GRR and ROT treatments increased the stem nitrogen uptake in early rice by 61.76% and 58.61% as compared to control, while CRR increased the nitrogen uptake by stem, leave and spike in late rice by 40.76%, 49.51% and 42.92%. In conclusion, CRR is more beneficial to increase DM accumulation and translocation by stem and leaf in double-cropping rice, and nitrogen uptake by rice plants.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135586478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guava (Psidium guajava L.) is the most cherished cultivated fruit species in the Myrtaceae family, and it is a perennial tree native to tropical America. The objective of this study was to determine the genetic variability of Psidium guajava in Cereté (Colombia) using SSR markers. DNA extraction was performed using the Mini-prep method with modifications. Nine microsatellites were amplified using the PCR Touchdown technique. Genetic-population parameters such as the number of alleles, effective number of alleles, observed heterozygosity, expected heterozygosity, fixation index, Hardy-Weinberg equilibrium, and polymorphic information content were calculated using PopGene 1.31 software. The number of alleles varied between 4 for markers mPgCIR13, mPgCIR20, mPgCIR23, and 8 for marker mPgCIR19, respectively. The average value of effective number of alleles was 3.722, observed heterozygosity was 0.217, and expected heterozygosity was 0.254. The average fixation index was 0.101. Hardy-Weinberg equilibrium tests revealed significant differences in the markers. The FIS coefficient had an average value of 0.385, the FIT coefficient showed an average of 0.490, and the FST coefficient had a value of 0.178. Genetic distance analysis showed that Mateo Gómez was closely related to Retiro de los Indios, while Rabolargo appeared to be the most distant population. The study revealed low genetic variability within and between the populations studied, possibly, reflecting the type of asexual propagation applied in guava crops.
番石榴(Psidium guajava L.)是番石榴科中最珍贵的栽培水果，是一种原产于热带美洲的多年生乔木。本研究的目的是利用SSR标记确定哥伦比亚ceret番石榴的遗传变异。DNA提取采用修改后的Mini-prep方法。采用PCR触地技术扩增9颗微卫星。利用PopGene 1.31软件计算等位基因数、有效等位基因数、观察杂合度、期望杂合度、固定指数、Hardy-Weinberg平衡、多态信息含量等遗传种群参数。标记mPgCIR13、mPgCIR20、mPgCIR23的等位基因数量分别为4个和mPgCIR19的8个。有效等位基因数平均值为3.722，观察杂合度为0.217，期望杂合度为0.254。平均固定指数为0.101。Hardy-Weinberg平衡检验显示标记物存在显著差异。FIS系数平均值为0.385,FIT系数平均值为0.490,FST系数平均值为0.178。遗传距离分析表明，Mateo Gómez与Retiro de los Indios亲缘关系密切，而Rabolargo似乎是距离最远的种群。研究表明，所研究群体内部和群体之间的遗传变异性较低，这可能反映了番石榴作物无性繁殖的类型。
{"title":"Molecular characterization of guava Psidium guajava in Cereté, Córdoba, Colombia","authors":"Enrique PARDO, Karen HERNÁNDEZ, Teodora CAVADÍA","doi":"10.15835/nbha51313305","DOIUrl":"https://doi.org/10.15835/nbha51313305","url":null,"abstract":"Guava (Psidium guajava L.) is the most cherished cultivated fruit species in the Myrtaceae family, and it is a perennial tree native to tropical America. The objective of this study was to determine the genetic variability of Psidium guajava in Cereté (Colombia) using SSR markers. DNA extraction was performed using the Mini-prep method with modifications. Nine microsatellites were amplified using the PCR Touchdown technique. Genetic-population parameters such as the number of alleles, effective number of alleles, observed heterozygosity, expected heterozygosity, fixation index, Hardy-Weinberg equilibrium, and polymorphic information content were calculated using PopGene 1.31 software. The number of alleles varied between 4 for markers mPgCIR13, mPgCIR20, mPgCIR23, and 8 for marker mPgCIR19, respectively. The average value of effective number of alleles was 3.722, observed heterozygosity was 0.217, and expected heterozygosity was 0.254. The average fixation index was 0.101. Hardy-Weinberg equilibrium tests revealed significant differences in the markers. The FIS coefficient had an average value of 0.385, the FIT coefficient showed an average of 0.490, and the FST coefficient had a value of 0.178. Genetic distance analysis showed that Mateo Gómez was closely related to Retiro de los Indios, while Rabolargo appeared to be the most distant population. The study revealed low genetic variability within and between the populations studied, possibly, reflecting the type of asexual propagation applied in guava crops.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135720313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paul SESTRAS, Sevastel MIRCEA, Sanda ROȘCA, Ștefan BILAȘCO, Tudor SĂLĂGEAN, Lucian O. DRAGOMIR, Mihai V. HERBEI, Simion BRUMA, Catalin SABOU, Rastko MARKOVIĆ, Shuraik KADER
The complex environmental process of soil erosion is crucial to sustainable land management and conservation. This study uses the Universal Soil Loss Equation (USLE) model to understand the intricate interactions that cause soil erosion in Cluj County, Romania, a region susceptible to complex erosion and landslide phenomenon. The established analysis depicts erosion patterns from localised hotspots to regions of relative stability, providing valuable insights into this critical issue. Spatial distribution maps with color-coded gradients show soil erosion risk and identify vulnerable regions, with temporal investigations depicting how environmental changes affect soil erosion, making it relevant to soil conservation and land management. A careful analysis of the USLE model's parameters (Ls, Cs, C, S, K) shows their soil erosion contributions. The erosion rates were graded in five classes in accordance to general practices of USLE modelling, which range from very-low, low, moderate, high and very-high susceptibility to soil erosion. While a significant majority of the county’s surface is represented by very-low and low erosion risk, several hotspots were identified with intense erosion processes that highlights the critical need to implement soil conservation measures in the area. Identifying erosion hotspots and conservation solutions encourages stakeholders to protect soils, and can provide policymakers with useful information for developing improved guidelines for soil and water conservation.
{"title":"GIS based soil erosion assessment using the USLE model for efficient land management: A case study in an area with diverse pedo-geomorphological and bioclimatic characteristics","authors":"Paul SESTRAS, Sevastel MIRCEA, Sanda ROȘCA, Ștefan BILAȘCO, Tudor SĂLĂGEAN, Lucian O. DRAGOMIR, Mihai V. HERBEI, Simion BRUMA, Catalin SABOU, Rastko MARKOVIĆ, Shuraik KADER","doi":"10.15835/nbha51313263","DOIUrl":"https://doi.org/10.15835/nbha51313263","url":null,"abstract":"The complex environmental process of soil erosion is crucial to sustainable land management and conservation. This study uses the Universal Soil Loss Equation (USLE) model to understand the intricate interactions that cause soil erosion in Cluj County, Romania, a region susceptible to complex erosion and landslide phenomenon. The established analysis depicts erosion patterns from localised hotspots to regions of relative stability, providing valuable insights into this critical issue. Spatial distribution maps with color-coded gradients show soil erosion risk and identify vulnerable regions, with temporal investigations depicting how environmental changes affect soil erosion, making it relevant to soil conservation and land management. A careful analysis of the USLE model's parameters (Ls, Cs, C, S, K) shows their soil erosion contributions. The erosion rates were graded in five classes in accordance to general practices of USLE modelling, which range from very-low, low, moderate, high and very-high susceptibility to soil erosion. While a significant majority of the county’s surface is represented by very-low and low erosion risk, several hotspots were identified with intense erosion processes that highlights the critical need to implement soil conservation measures in the area. Identifying erosion hotspots and conservation solutions encourages stakeholders to protect soils, and can provide policymakers with useful information for developing improved guidelines for soil and water conservation.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135720312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amer M. ABDELAZIZ, Mohamed H. SHARAF, Amr H. HASHEM, Abdulaziz A. AL-ASKAR, Samy A. MAREY, Fares A. MOHAMED, Mohamed N. ABDELSTAR, Mosad A. ZAKI, Hamada ABDELGAWAD, Mohamed S. ATTIA
Plant growth promoting fungi (PGPF) were employed in the present study to biocontrol Fusarium wilt disease in pepper plants. Two of the five fungal isolates were chosen based on biochemical characteristics such as their production of hydrocyanic acid, siderophores, and IAA, phosphate solubilization, and in vitro antifungal activities. The most potent fungal isolates were identified as Penicillium expansum (P. expansum) and Trichoderma harzianum (T. harzianum). Using GC-MS, it was found that PGPF extracts contain compounds with antifungal activity, antioxidants, and plant growth stimulators. The combined effect of T. harzianum and P. expansum increased the protection against fusarial wilt by (76.74%), followed by T. harzianum by (50%), then P. expansum by (17.64%). Significant improvement because of using the mixture (T. harzianum and P. expansum) showed an increase in shoot length (59.4%), root length (129%), and number of leaves (52.6%). Chlorophyll A and B levels in infected plants were consistently raised by 28.71% and 67.58%, respectively; as a result of application the mixture (T. harzianum and P. expansum). Also, there was an increase in soluble proteins and carbohydrates in infected plants treated with (T. harzianum) by 25.42% and 31.78% over untreated infected plants, respectively. It could be recommended that the use of targeted PGPF strains, especially a mixture of T. harzianum and P. expansum could be commercially used as therapeutic nutrients against Fusarium wilt of pepper plants.
{"title":"Biocontrol of Fusarium wilt disease in pepper plant by plant growth promoting Penicillium expansum and Trichoderma harzianum","authors":"Amer M. ABDELAZIZ, Mohamed H. SHARAF, Amr H. HASHEM, Abdulaziz A. AL-ASKAR, Samy A. MAREY, Fares A. MOHAMED, Mohamed N. ABDELSTAR, Mosad A. ZAKI, Hamada ABDELGAWAD, Mohamed S. ATTIA","doi":"10.15835/nbha51313302","DOIUrl":"https://doi.org/10.15835/nbha51313302","url":null,"abstract":"Plant growth promoting fungi (PGPF) were employed in the present study to biocontrol Fusarium wilt disease in pepper plants. Two of the five fungal isolates were chosen based on biochemical characteristics such as their production of hydrocyanic acid, siderophores, and IAA, phosphate solubilization, and in vitro antifungal activities. The most potent fungal isolates were identified as Penicillium expansum (P. expansum) and Trichoderma harzianum (T. harzianum). Using GC-MS, it was found that PGPF extracts contain compounds with antifungal activity, antioxidants, and plant growth stimulators. The combined effect of T. harzianum and P. expansum increased the protection against fusarial wilt by (76.74%), followed by T. harzianum by (50%), then P. expansum by (17.64%). Significant improvement because of using the mixture (T. harzianum and P. expansum) showed an increase in shoot length (59.4%), root length (129%), and number of leaves (52.6%). Chlorophyll A and B levels in infected plants were consistently raised by 28.71% and 67.58%, respectively; as a result of application the mixture (T. harzianum and P. expansum). Also, there was an increase in soluble proteins and carbohydrates in infected plants treated with (T. harzianum) by 25.42% and 31.78% over untreated infected plants, respectively. It could be recommended that the use of targeted PGPF strains, especially a mixture of T. harzianum and P. expansum could be commercially used as therapeutic nutrients against Fusarium wilt of pepper plants.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135720314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To identify the propagation mechanisms and the adaptive strategies of oat seedlings exposed to NaCl, NaHCO3 and Na2CO3 the main salts in the soils of the salt-alkali grasslands of the Songnen Plain of China, growth rates and physiological indices of oat seedlings were measured in plants grown in soils with different concentrations (48-144 mmol L-1) of the three salts. The results demonstrated that although oat seedlings survival rates were unaffected by NaCl stress, the tiller number, plant height, and shoot and root dry weights decreased with increasing salt concentration, in the order of Na2CO3＞NaHCO3＞NaCl. In addition, propagation mechanisms higher concentrations of Na+ accumulated in the shoots and roots of oat seedlings under Na2CO3 stress and NaHCO3 stress than in seedlings under NaCl stress. Reductions in concentrations of K+ were also greater under both Na2CO3 and NaHCO3 stress than NaCl, especially in the roots. Large amounts of Cl－ and proline were found to accumulate in oat seedlings, most likely as a strategy for maintaining osmotic and ionic homeostasis under NaCl stress.
{"title":"Physiological responses and adoptive mechanisms in oat against three levels of salt stress","authors":"Zhan-Wu GAO, Sun PEILIANG, Yan-Hui CUI, Asma HANIF, Zong-Ze YANG, Rui-Li LI, Chun-Sheng MU, Adnan RASHEED","doi":"10.15835/nbha51313249","DOIUrl":"https://doi.org/10.15835/nbha51313249","url":null,"abstract":"To identify the propagation mechanisms and the adaptive strategies of oat seedlings exposed to NaCl, NaHCO3 and Na2CO3 the main salts in the soils of the salt-alkali grasslands of the Songnen Plain of China, growth rates and physiological indices of oat seedlings were measured in plants grown in soils with different concentrations (48-144 mmol L-1) of the three salts. The results demonstrated that although oat seedlings survival rates were unaffected by NaCl stress, the tiller number, plant height, and shoot and root dry weights decreased with increasing salt concentration, in the order of Na2CO3＞NaHCO3＞NaCl. In addition, propagation mechanisms higher concentrations of Na+ accumulated in the shoots and roots of oat seedlings under Na2CO3 stress and NaHCO3 stress than in seedlings under NaCl stress. Reductions in concentrations of K+ were also greater under both Na2CO3 and NaHCO3 stress than NaCl, especially in the roots. Large amounts of Cl－ and proline were found to accumulate in oat seedlings, most likely as a strategy for maintaining osmotic and ionic homeostasis under NaCl stress.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135720531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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