Lianah Kuswanto, Noor Amalia Chusna, E. Purnomo, Krisantini, M. Ahmad
Indonesia has diverse flora and fauna, and many species remain largely undiscovered. Documentation and identification of threatened wild ornamental species are increasingly difficult due to ongoing exploitation and land conversion. Mount Prau is one of the popular destinations in Central Java, Indonesia for tourism. Understanding plant biodiversity has enormous value for the economy, ecology, culture, science, and recreation. Our study is aimed to record the diversity and identify the flowering plant species in their native habitat at Mount Prau, Central Java, Indonesia. Our field surveys demonstrated that Mount Prau has abundant wild ornamental plants with wide diversity of taxa, growth habits, and forms. A total of 103 species representing 51 families and 95 genera are identified including trees, shrubs, herbs, and lianas. We also found that among the plant species found in Mount Prau, 24 have morphological characters suitable to be cultivated as ornamental flowers, and 12 as ornamental foliages, and 63 species are medicinal plants. The ornamental criteria of these species were based on the literature describing the morphological and unique characters of leaves and flowers that made them potential to be developed as ornamental plants. In this paper we have provided the current conservation status of the plant species identified and recommendations on their conservation. This study provides baseline data of species found in the Mount Prau areas, and this information could be helpful for further conservations efforts and initiatives.
{"title":"Identification and documentation of wild plant species with ornamental potentials at Mount Prau, Central Java, Indonesia","authors":"Lianah Kuswanto, Noor Amalia Chusna, E. Purnomo, Krisantini, M. Ahmad","doi":"10.31219/osf.io/ywkbz","DOIUrl":"https://doi.org/10.31219/osf.io/ywkbz","url":null,"abstract":"Indonesia has diverse flora and fauna, and many species remain largely undiscovered. Documentation and identification of threatened wild ornamental species are increasingly difficult due to ongoing exploitation and land conversion. Mount Prau is one of the popular destinations in Central Java, Indonesia for tourism. Understanding plant biodiversity has enormous value for the economy, ecology, culture, science, and recreation. Our study is aimed to record the diversity and identify the flowering plant species in their native habitat at Mount Prau, Central Java, Indonesia. Our field surveys demonstrated that Mount Prau has abundant wild ornamental plants with wide diversity of taxa, growth habits, and forms. A total of 103 species representing 51 families and 95 genera are identified including trees, shrubs, herbs, and lianas. We also found that among the plant species found in Mount Prau, 24 have morphological characters suitable to be cultivated as ornamental flowers, and 12 as ornamental foliages, and 63 species are medicinal plants. The ornamental criteria of these species were based on the literature describing the morphological and unique characters of leaves and flowers that made them potential to be developed as ornamental plants. In this paper we have provided the current conservation status of the plant species identified and recommendations on their conservation. This study provides baseline data of species found in the Mount Prau areas, and this information could be helpful for further conservations efforts and initiatives.","PeriodicalId":19699,"journal":{"name":"Ornamental Horticulture","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47331336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2447-536x.v28i1.2411
V. B. P. Paiva Neto, M. A. Torrezan, Manoela F.F. da Silva, Daly Roxana Castro Padilha, J. Borel, Monica Cristina Rezende Zuffo-Borges
Abstract Cycnoches haagii Barb. Rodr. is an epiphytic orchid very targeted by collectors, but no reference was found in the literature about its reproductive biology. Thus, the purpose of this study was to obtain initial information regarding pollination types and its influence on seed viability of this native orchid of the Brazilian Cerrado, in order to enable future propagation and preservation programs. Pollination among flowers of the same plant (geitonogamy) or different plants (xenogamy) were carried out. Seeds extracted from the capsules were sown in B&G medium, with full and half strength. Seeds from geitonogamic resulted in 25% of albino protocorms and consequently in albino seedlings. This phenomenon did not occur in seedlings derived from xenogamic pollination. Pigment analysis showed that even the albino seedlings presented chlorophylls and carotenoids, however, in significantly minor concentrations, 16% and 37% respectively, in relation to green seedlings. Geitonogamic and xenogamic pollinations resulted in C. haagii viable seeds with high germination percentage (90%) under in vitro conditions. The germination of seeds from xenogamic pollination resulted in chlorophyll or normal seedlings only, and can be recommended at conservation programs. On the other hand, although geitonogamic pollination should be avoided at conservation programs of this orchid species as it leads to albino seedlings, it showed a very interesting system to obtain seedlings with this phenotype, an interesting plant material to future investigation.
{"title":"Self-pollination of the orchid Cycnoches haagii from Brazilian Cerrado results in albino phenotype seedlings","authors":"V. B. P. Paiva Neto, M. A. Torrezan, Manoela F.F. da Silva, Daly Roxana Castro Padilha, J. Borel, Monica Cristina Rezende Zuffo-Borges","doi":"10.1590/2447-536x.v28i1.2411","DOIUrl":"https://doi.org/10.1590/2447-536x.v28i1.2411","url":null,"abstract":"Abstract Cycnoches haagii Barb. Rodr. is an epiphytic orchid very targeted by collectors, but no reference was found in the literature about its reproductive biology. Thus, the purpose of this study was to obtain initial information regarding pollination types and its influence on seed viability of this native orchid of the Brazilian Cerrado, in order to enable future propagation and preservation programs. Pollination among flowers of the same plant (geitonogamy) or different plants (xenogamy) were carried out. Seeds extracted from the capsules were sown in B&G medium, with full and half strength. Seeds from geitonogamic resulted in 25% of albino protocorms and consequently in albino seedlings. This phenomenon did not occur in seedlings derived from xenogamic pollination. Pigment analysis showed that even the albino seedlings presented chlorophylls and carotenoids, however, in significantly minor concentrations, 16% and 37% respectively, in relation to green seedlings. Geitonogamic and xenogamic pollinations resulted in C. haagii viable seeds with high germination percentage (90%) under in vitro conditions. The germination of seeds from xenogamic pollination resulted in chlorophyll or normal seedlings only, and can be recommended at conservation programs. On the other hand, although geitonogamic pollination should be avoided at conservation programs of this orchid species as it leads to albino seedlings, it showed a very interesting system to obtain seedlings with this phenotype, an interesting plant material to future investigation.","PeriodicalId":19699,"journal":{"name":"Ornamental Horticulture","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44398420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2447-536x.v28i1.2388
João Gilberto Meza Ucella-Filho, F. Lucas, Débora de Melo Almeida, Bruna Rafaella Ferreira da Silva, Dayane de Melo Almeida, Dáfilla Yara Oliveira de Brito, T. Azevêdo
Abstract Urban forests are responsible for a lot of benefits, and can be used as a tool to ex-situ conservation of native species. Our study investigated a public urban forest aiming to provide subside to management projects in order to allow the accurate fulfillment of social, aesthetic and ecological functions displayed by trees. Our survey was conducted in 6 public squares and 5 gardens in the Jardins neighborhood. We classified the trees according to their phytogeographic origins and seed dispersal habit. The Shannon-Weaver (H’) index, Simpson’s dominance (D’) and Pielou’s equability (J) were determined. A total of 507 individuals were sampled, located in 12 botanical families and 29 species, and 13 of them were classified as native and 16 as exotic. The family with higher species richness and number of species was Fabaceae (36%). Regarding the seed dispersal syndrome, the most representative was zoochory (52%). The ecological index showed medium species diversity (H’=2.2284), high dominance (D’=0.7899) and medium number of individuals (J) 0.6552. Although the Jardins neighborhood was planned, the urban forest in this area still needs improvement; such as incentive measures that can promote biodiversity and the application of ex-situ conservation.
{"title":"Biodiverse neighborhoods: an ex-situ conservation tool","authors":"João Gilberto Meza Ucella-Filho, F. Lucas, Débora de Melo Almeida, Bruna Rafaella Ferreira da Silva, Dayane de Melo Almeida, Dáfilla Yara Oliveira de Brito, T. Azevêdo","doi":"10.1590/2447-536x.v28i1.2388","DOIUrl":"https://doi.org/10.1590/2447-536x.v28i1.2388","url":null,"abstract":"Abstract Urban forests are responsible for a lot of benefits, and can be used as a tool to ex-situ conservation of native species. Our study investigated a public urban forest aiming to provide subside to management projects in order to allow the accurate fulfillment of social, aesthetic and ecological functions displayed by trees. Our survey was conducted in 6 public squares and 5 gardens in the Jardins neighborhood. We classified the trees according to their phytogeographic origins and seed dispersal habit. The Shannon-Weaver (H’) index, Simpson’s dominance (D’) and Pielou’s equability (J) were determined. A total of 507 individuals were sampled, located in 12 botanical families and 29 species, and 13 of them were classified as native and 16 as exotic. The family with higher species richness and number of species was Fabaceae (36%). Regarding the seed dispersal syndrome, the most representative was zoochory (52%). The ecological index showed medium species diversity (H’=2.2284), high dominance (D’=0.7899) and medium number of individuals (J) 0.6552. Although the Jardins neighborhood was planned, the urban forest in this area still needs improvement; such as incentive measures that can promote biodiversity and the application of ex-situ conservation.","PeriodicalId":19699,"journal":{"name":"Ornamental Horticulture","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41418608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2447-536X.V28I1.2425
A. Escandon
Biodiversity is the set of living organisms that make up our battered planet and their interactions with the environment. It is made up of the Kingdoms: Animalia, Plantae, Fungi, Protista, and Monera. It provides the energy resources necessary to sustain the planet and our civilization; it is the basis of agriculture, source of all crops, and species of domestic livestock since the beginning of human civilization.[...]
{"title":"A point of view on genetic resources and plant breeding","authors":"A. Escandon","doi":"10.1590/2447-536X.V28I1.2425","DOIUrl":"https://doi.org/10.1590/2447-536X.V28I1.2425","url":null,"abstract":"Biodiversity is the set of living organisms that make up our battered planet and their interactions with the environment. It is made up of the Kingdoms: Animalia, Plantae, Fungi, Protista, and Monera. It provides the energy resources necessary to sustain the planet and our civilization; it is the basis of agriculture, source of all crops, and species of domestic livestock since the beginning of human civilization.[...]","PeriodicalId":19699,"journal":{"name":"Ornamental Horticulture","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46303777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2447-536X.V28I1.2412
L. J. Vanegas-Cano, S. T. Martínez-Peralta, E. Coy-Barrera, H. D. Ardila-Barrantes
Abstract The vascular wilting caused by Fusarium oxysporum f. sp. dianthi (Fod) is the most relevant disease for carnation cultivation. Understanding the biochemical mechanisms involved in resistance to Fod will allow the development of new disease control strategies. In this research, the levels of some phytohormones such as salicylic acid (SA), methyl salicylate (MeSA), and methyl jasmonate (MeJA) were evaluated in symplast of carnation roots infected with this pathogen. The accumulation of these hormones was then correlated with the expression levels of symplastic peroxidases, enzymes involved in the plant resistance against pathogen during interaction. Our results suggested that pathogen infection causes a differential accumulation of SA, MeSA, and MeJA in a resistant cultivar (i.e. ‘Golem’), being earlier and higher than that observed in a susceptible one (i.e. ‘Solex’). Simultaneously, an increase of guaiacol peroxidase enzymatic activity (GPX) and transcriptional levels of a gene coding for a symplastic peroxidase were presented as part of the resistance response. The positive statistical correlation between the accumulation of SA and MeJA and the expression of peroxidases (GPX activity and mRNA levels) indicates the possible cellular relationship of these phenomena during the activation of the resistance to Fod. Our findings suggested some hormonal signaling mechanisms acting at the roots during the regulation of the biochemical response associated with resistance against Fod.
摘要由尖孢镰刀菌(Fusarium oxysporum f. sp. dianthi)引起的维管枯萎病是康乃馨栽培最相关的病害。了解食品抗性的生化机制将有助于开发新的疾病控制策略。本研究测定了感染该病原菌的康乃馨根共质体中水杨酸(SA)、水杨酸甲酯(MeSA)和茉莉酸甲酯(MeJA)等植物激素的含量。这些激素的积累与共塑过氧化物酶的表达水平相关，共塑过氧化物酶在相互作用过程中参与植物对病原体的抗性。我们的研究结果表明，病原菌感染导致抗性品种(如“Golem”)中SA、MeSA和MeJA的差异积累，比敏感品种(如“Solex”)中观察到的更早和更高。同时，愈创木酚过氧化物酶酶活性(GPX)和编码共塑过氧化物酶基因转录水平的增加被认为是抗性反应的一部分。SA和MeJA的积累与过氧化物酶的表达(GPX活性和mRNA水平)呈正相关，表明这些现象在食品抗性激活过程中可能存在细胞关系。我们的研究结果表明，在与食品抗性相关的生化反应的调节过程中，一些激素信号机制在根部起作用。
{"title":"Plant hormones accumulation and its relationship with symplastic peroxidases expression during carnation-Fusarium oxysporum interaction","authors":"L. J. Vanegas-Cano, S. T. Martínez-Peralta, E. Coy-Barrera, H. D. Ardila-Barrantes","doi":"10.1590/2447-536X.V28I1.2412","DOIUrl":"https://doi.org/10.1590/2447-536X.V28I1.2412","url":null,"abstract":"Abstract The vascular wilting caused by Fusarium oxysporum f. sp. dianthi (Fod) is the most relevant disease for carnation cultivation. Understanding the biochemical mechanisms involved in resistance to Fod will allow the development of new disease control strategies. In this research, the levels of some phytohormones such as salicylic acid (SA), methyl salicylate (MeSA), and methyl jasmonate (MeJA) were evaluated in symplast of carnation roots infected with this pathogen. The accumulation of these hormones was then correlated with the expression levels of symplastic peroxidases, enzymes involved in the plant resistance against pathogen during interaction. Our results suggested that pathogen infection causes a differential accumulation of SA, MeSA, and MeJA in a resistant cultivar (i.e. ‘Golem’), being earlier and higher than that observed in a susceptible one (i.e. ‘Solex’). Simultaneously, an increase of guaiacol peroxidase enzymatic activity (GPX) and transcriptional levels of a gene coding for a symplastic peroxidase were presented as part of the resistance response. The positive statistical correlation between the accumulation of SA and MeJA and the expression of peroxidases (GPX activity and mRNA levels) indicates the possible cellular relationship of these phenomena during the activation of the resistance to Fod. Our findings suggested some hormonal signaling mechanisms acting at the roots during the regulation of the biochemical response associated with resistance against Fod.","PeriodicalId":19699,"journal":{"name":"Ornamental Horticulture","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44400640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2447-536X.V28I1.2402
Cristiane Gonçalves Souza, S. Ramos, S. Nietsche, C. C. F. Possobom, E. Almeida, M. C. Pereira
Abstract Adenium obesum (Forssk.) Roem. & Schult., popularly known as desert rose, has become a valuable ornamental plant. In floriculture, the production of hybrids is prioritized. Hence, knowledge on floral biology and sexual reproduction of the target species is fundamental. The objectives of this study were: (1) to test sucrose concentrations and temperatures for in vitro germination of A. obesum pollen grains; (2) to identify the effect of temperature on the viability of A. obesum pollen grains; and (3) to evaluate the viability of pollen grains and stigma receptivity in pre-anthesis, at flower opening, and 72 h post-flower opening in three accessions of A. obesum. A significant relationship between temperatures and sucrose concentrations was observed in the in vitro germination test. The highest percentage of in vitro germination of pollen grains, 39.81%, was observed at an estimated temperature of 26.05 °C. Desert rose accessions maintained in biochemical oxygen demand (BOD) chambers at 30 °C during a 16-h light photoperiod showed faster flowering, and temperatures ≥ 25 °C induced pollen grain viability percentages above 69%. Temperature is one of the most important abiotic factors, influencing mainly in pollen germination, pollen tube growing and in efficiency fertilization. The ICA-wd accession stood out and can be considered a pollen donor in artificial pollination. The stigmas of flowers were receptive from a day before flower opening until three days after. The two parameters presented above, stigma receptivity and pollen viability, allow inferences about the appropriate time for successful pollination and subsequent fertilization in desert roses.
{"title":"Viability of pollen grains and stigma receptivity in Desert Rose","authors":"Cristiane Gonçalves Souza, S. Ramos, S. Nietsche, C. C. F. Possobom, E. Almeida, M. C. Pereira","doi":"10.1590/2447-536X.V28I1.2402","DOIUrl":"https://doi.org/10.1590/2447-536X.V28I1.2402","url":null,"abstract":"Abstract Adenium obesum (Forssk.) Roem. & Schult., popularly known as desert rose, has become a valuable ornamental plant. In floriculture, the production of hybrids is prioritized. Hence, knowledge on floral biology and sexual reproduction of the target species is fundamental. The objectives of this study were: (1) to test sucrose concentrations and temperatures for in vitro germination of A. obesum pollen grains; (2) to identify the effect of temperature on the viability of A. obesum pollen grains; and (3) to evaluate the viability of pollen grains and stigma receptivity in pre-anthesis, at flower opening, and 72 h post-flower opening in three accessions of A. obesum. A significant relationship between temperatures and sucrose concentrations was observed in the in vitro germination test. The highest percentage of in vitro germination of pollen grains, 39.81%, was observed at an estimated temperature of 26.05 °C. Desert rose accessions maintained in biochemical oxygen demand (BOD) chambers at 30 °C during a 16-h light photoperiod showed faster flowering, and temperatures ≥ 25 °C induced pollen grain viability percentages above 69%. Temperature is one of the most important abiotic factors, influencing mainly in pollen germination, pollen tube growing and in efficiency fertilization. The ICA-wd accession stood out and can be considered a pollen donor in artificial pollination. The stigmas of flowers were receptive from a day before flower opening until three days after. The two parameters presented above, stigma receptivity and pollen viability, allow inferences about the appropriate time for successful pollination and subsequent fertilization in desert roses.","PeriodicalId":19699,"journal":{"name":"Ornamental Horticulture","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49281055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2447-536X.V28I1.2403
M. Kahraman, Y. Mendi, Ş. Karabıyık, H. Lütken, B. T. Favero
Abstract Kalanchoë cultivars rank as one of the most sold potted ornamental plants in the world. Among its key features that sustain high market interest are the long flowering period, abundance of flowers, thick and glossy leaves, easy maintenance and less water requirement compared to other potted plants. In breeding studies of Kalanchoë, plants with different flower colors such as white, cream, yellow, orange, red, pink and purple were developed. Moreover, double-flowered cultivars (comprising a large number of petals), more compact and cultivars with larger flowers were also obtained. Novel morphological characteristics are always in high demand in the market of ornamental plants. Increasing the variation in the gene pool with different species played a major role in the development of these characteristics. Nowadays, not only potted cultivars are launched but also cut flower cultivars have been developed and presented to consumers. Besides conventional breeding and interspecific hybridization, biotechnological methods, which have a promising future, are used to develop morphological characteristics of Kalanchoë.
{"title":"Kalanchoë Breeding: Past, Present and Future","authors":"M. Kahraman, Y. Mendi, Ş. Karabıyık, H. Lütken, B. T. Favero","doi":"10.1590/2447-536X.V28I1.2403","DOIUrl":"https://doi.org/10.1590/2447-536X.V28I1.2403","url":null,"abstract":"Abstract Kalanchoë cultivars rank as one of the most sold potted ornamental plants in the world. Among its key features that sustain high market interest are the long flowering period, abundance of flowers, thick and glossy leaves, easy maintenance and less water requirement compared to other potted plants. In breeding studies of Kalanchoë, plants with different flower colors such as white, cream, yellow, orange, red, pink and purple were developed. Moreover, double-flowered cultivars (comprising a large number of petals), more compact and cultivars with larger flowers were also obtained. Novel morphological characteristics are always in high demand in the market of ornamental plants. Increasing the variation in the gene pool with different species played a major role in the development of these characteristics. Nowadays, not only potted cultivars are launched but also cut flower cultivars have been developed and presented to consumers. Besides conventional breeding and interspecific hybridization, biotechnological methods, which have a promising future, are used to develop morphological characteristics of Kalanchoë.","PeriodicalId":19699,"journal":{"name":"Ornamental Horticulture","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48258371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.1590/2447-536x.v27i4.2320
Narges Asadi, H. Zarei, S. H. Hashemi-petroudi, S. J. Mousavizadeh
Abstract In vitro culture of twin-scaling explants of Galanthus transcaucasicus with different concentrations of plant growth regulators (PGRs) including 0.5, 1, 2, 3, 4, 6, 8, and 10 mg L-1 naphthaleneacetic acid (NAA) and 0.5, 1, 2, 3, and 4 mg L-1 benzyladenine (BA) was studied. After 18 weeks, the number of regenerated bulblets and intensity of callus was measured. Subsequently, bulblets were transferred to a medium with 0.5, 1, 2, 3, and 4 mg L-1 NAA and 0.5, 1, 2, 3, and 4 mg L-1 BA and, after 15 weeks, the bulblets length and diameter were measured. The highest intensity of callus was obtained on 4 mg L-1 NAA or 8 mg L-1 NAA with 1 mg L-1 BA. The highest number of regenerated bulblets was detected with 6 mg L-1 NAA and 2 mg L-1 BA. The highest diameter of bulblets occurred on four mgL-1 NAA (9.4 mm), while the lowest was observed on 0.5 mg L-1 BA (1.83 mm). The analysis of genetic variation using ISSR revealed that there was no somaclonal variation among the regenerated plants from BA and low level of NAA, but there was a significant somaclonal variation at high concentrations of NAA.
{"title":"Micropropagation and assessment of somaclonal variation in Galanthus transcaucasicus in vitro plantlets","authors":"Narges Asadi, H. Zarei, S. H. Hashemi-petroudi, S. J. Mousavizadeh","doi":"10.1590/2447-536x.v27i4.2320","DOIUrl":"https://doi.org/10.1590/2447-536x.v27i4.2320","url":null,"abstract":"Abstract In vitro culture of twin-scaling explants of Galanthus transcaucasicus with different concentrations of plant growth regulators (PGRs) including 0.5, 1, 2, 3, 4, 6, 8, and 10 mg L-1 naphthaleneacetic acid (NAA) and 0.5, 1, 2, 3, and 4 mg L-1 benzyladenine (BA) was studied. After 18 weeks, the number of regenerated bulblets and intensity of callus was measured. Subsequently, bulblets were transferred to a medium with 0.5, 1, 2, 3, and 4 mg L-1 NAA and 0.5, 1, 2, 3, and 4 mg L-1 BA and, after 15 weeks, the bulblets length and diameter were measured. The highest intensity of callus was obtained on 4 mg L-1 NAA or 8 mg L-1 NAA with 1 mg L-1 BA. The highest number of regenerated bulblets was detected with 6 mg L-1 NAA and 2 mg L-1 BA. The highest diameter of bulblets occurred on four mgL-1 NAA (9.4 mm), while the lowest was observed on 0.5 mg L-1 BA (1.83 mm). The analysis of genetic variation using ISSR revealed that there was no somaclonal variation among the regenerated plants from BA and low level of NAA, but there was a significant somaclonal variation at high concentrations of NAA.","PeriodicalId":19699,"journal":{"name":"Ornamental Horticulture","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45847576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.1590/2447-536x.v27i4.2389
Julia Martella de Almeida, Cristiane Calaboni, P. H. V. Rodrigues
Abstract The lisianthus culture is demanded to produce cut flowers and pot vases, grown in a greenhouse using advanced techniques. The present study aimed to evaluate the pigments of different lisianthus cultivars in different photoselective shade nets. The cultivars Flare Deep Rose (pink), Echo Blue BL (purple) and Allemande White BI (white) were cultivated under light-diffusing polyethylene film cover and blue and red color photoselective nets with a shading capacity of 30%, in addition to the control treatment, without the use of the net. The results showed higher values of chlorophyll b and total in the red photoselective net, independent of the cultivar. Leaf colorimetry showed the most intense shade of green was from the cultivar Echo Blue BL. On the other hand, the control showed more intensity, with no significant difference in the leaf green intensity on the blue and red shade nets. In the petals, the cultivar Echo Blue BL showed a more intense blue shade in the red net, however low grade of anthocyanin than in the blue one. In the anthocyanin pigment, this cultivar had lower levels in the treatment of the red net. The results indicate that the use of photoselective shade nets in the culture of lisianthus changes the content of the pigments studied, in varying intensity, depending on the cultivar.
摘要:在温室中采用先进的栽培技术，对万寿菊的栽培要求是生产切花和花盆。本研究的目的是评价不同品种的百叶菊在不同光选择性遮荫网下的色素。在对照处理的基础上，采用散光聚乙烯膜覆盖和遮光能力为30%的蓝、红两色光选择网，在不使用网的情况下，培育出粉红色的Flare Deep Rose、紫色的Echo Blue BL和白色的Allemande White BI。结果表明，红色光选择网中叶绿素b和总含量较高，与品种无关。叶片比色分析结果显示，回声蓝BL的绿光强度最大，而对照的绿光强度更大，在蓝网和红网上的绿光强度差异不显著。在花瓣中，回声蓝BL在红色网中呈现出更强烈的蓝色阴影，但花青素含量低于蓝色网。在花青素色素方面，该品种在红网处理中含量较低。结果表明，光选择遮荫网的使用改变了所研究的色素含量，其强度取决于品种。
{"title":"Pigments in flower stems of lisianthus under different photoselective shade nets","authors":"Julia Martella de Almeida, Cristiane Calaboni, P. H. V. Rodrigues","doi":"10.1590/2447-536x.v27i4.2389","DOIUrl":"https://doi.org/10.1590/2447-536x.v27i4.2389","url":null,"abstract":"Abstract The lisianthus culture is demanded to produce cut flowers and pot vases, grown in a greenhouse using advanced techniques. The present study aimed to evaluate the pigments of different lisianthus cultivars in different photoselective shade nets. The cultivars Flare Deep Rose (pink), Echo Blue BL (purple) and Allemande White BI (white) were cultivated under light-diffusing polyethylene film cover and blue and red color photoselective nets with a shading capacity of 30%, in addition to the control treatment, without the use of the net. The results showed higher values of chlorophyll b and total in the red photoselective net, independent of the cultivar. Leaf colorimetry showed the most intense shade of green was from the cultivar Echo Blue BL. On the other hand, the control showed more intensity, with no significant difference in the leaf green intensity on the blue and red shade nets. In the petals, the cultivar Echo Blue BL showed a more intense blue shade in the red net, however low grade of anthocyanin than in the blue one. In the anthocyanin pigment, this cultivar had lower levels in the treatment of the red net. The results indicate that the use of photoselective shade nets in the culture of lisianthus changes the content of the pigments studied, in varying intensity, depending on the cultivar.","PeriodicalId":19699,"journal":{"name":"Ornamental Horticulture","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48908513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.1590/2447-536x.v27i4.2401
P. A. Buffon, N. T. Schwab, N. Streck, L. O. Uhlmann, Elton Ferreira Lima, Paulo Marcks, Regina Tomiozzo
Abstract Vernalization, natural or artificial, is a physiological requirement of some plants to meet the need for low temperatures for its complete development to occur. The objective of this article is to describe a protocol of transforming a domestic refrigerator into a BOD (Biochemical Oxygen Demand) prototype aimed at vernalizing propagating materials statice crop. The first step is to install a temperature controller to maintain a constant temperature inside the refrigerator (10 °C). Thinking of seedling vernalization, it is still necessary to install a system of lights inside the refrigerator. The control of the time that the lights remain on inside the prototype is carried out by installing a Timer adjusted so that the lights remained on, uninterruptedly, for 10 hours. To test the effective ness of the prototype, an on-farm experiment was carried out with the statice (Limonium sinuatum L.) crop at 5 locations in Rio Grande do Sul, Brazil. The seedlings were vernalized at an internal temperature of 10 °C and photoperiod of 10 hours for 3 weeks. The duration of this experiment was 8 months and at the end of this observed that statice plants were correctly vernalized because the plants emitted flower stems and showed satisfactory development throughout the growing cycle. Therefore, that the adaptation of a domestic refrigerator as a BOD economically viable and easy mounting prototype is possible. Being an excellent alternative to small producers.
{"title":"Transforming a home refrigerator into a BOD prototype for statice vernalization","authors":"P. A. Buffon, N. T. Schwab, N. Streck, L. O. Uhlmann, Elton Ferreira Lima, Paulo Marcks, Regina Tomiozzo","doi":"10.1590/2447-536x.v27i4.2401","DOIUrl":"https://doi.org/10.1590/2447-536x.v27i4.2401","url":null,"abstract":"Abstract Vernalization, natural or artificial, is a physiological requirement of some plants to meet the need for low temperatures for its complete development to occur. The objective of this article is to describe a protocol of transforming a domestic refrigerator into a BOD (Biochemical Oxygen Demand) prototype aimed at vernalizing propagating materials statice crop. The first step is to install a temperature controller to maintain a constant temperature inside the refrigerator (10 °C). Thinking of seedling vernalization, it is still necessary to install a system of lights inside the refrigerator. The control of the time that the lights remain on inside the prototype is carried out by installing a Timer adjusted so that the lights remained on, uninterruptedly, for 10 hours. To test the effective ness of the prototype, an on-farm experiment was carried out with the statice (Limonium sinuatum L.) crop at 5 locations in Rio Grande do Sul, Brazil. The seedlings were vernalized at an internal temperature of 10 °C and photoperiod of 10 hours for 3 weeks. The duration of this experiment was 8 months and at the end of this observed that statice plants were correctly vernalized because the plants emitted flower stems and showed satisfactory development throughout the growing cycle. Therefore, that the adaptation of a domestic refrigerator as a BOD economically viable and easy mounting prototype is possible. Being an excellent alternative to small producers.","PeriodicalId":19699,"journal":{"name":"Ornamental Horticulture","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41715186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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