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Total phenolics, flavonoids, antioxidant activity, and α-glucosidase inhibitory activity of ornamental pepper and several other lines 观赏辣椒和其他几种品系的总酚类、黄酮类、抗氧化活性和α-葡萄糖苷酶抑制活性
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2424
Z. D. Sahid, M. Syukur, A. Maharijaya, W. Nurcholis
Abstract Ornamental chili is a type of chili plant that favors the value of beauty but still has a spicy taste. It generally has a purple fruit color because they contain anthocyanins. The biochemical content in ornamental chili is believed to have benefits for human health. This study aims to identify several biochemical compounds in several genotypes of ornamental chili and pure lines. The study was conducted using the microplate method which was repeated three times. The research design used was a completely randomized design with a single factor genotype. Sixteen genotypes of chili were used, consisting of 4 genotypes of ornamental chilies and 12 genotypes of pure lines. The results showed that the ornamental chili genotype Nazla IPB contained 83.27% α-glucosidase inhibitory activities. The pure lines genotype F10 145174-9-7-1-5-3-1-2-5 contained the second-largest α-glucosidase inhibitory activities (80.67%) and the highest antioxidant DPPH (15.40 μmol TE g.-1 extract). The highest antioxidant of the FRAP method was shown in Adelina IPB’s ornamental chili. The correlation between biochemical parameters showed that DPPH-AGI was significantly positively correlated and FRAP-AGI was negatively correlated. It is concluded that the information in this study can be the basis for the development of functional ornamental plants in future ornamental chili breeding research.
摘要观赏性辣椒是一种既讲究美观又具有辛辣味道的辣椒植物。它通常有紫色的果实颜色,因为它们含有花青素。观赏辣椒中的生化成分被认为对人体健康有益。本研究旨在鉴定观赏辣椒的几个基因型和纯种系中的几种生化化合物。采用微孔板法,重复三次。本研究采用单因素基因型的完全随机设计。采用16个辣椒基因型,其中观赏辣椒基因型4个,纯种辣椒基因型12个。结果表明,纳兹拉IPB型观赏辣椒α-葡萄糖苷酶抑制活性为83.27%。基因型F10 145174-9-7-1-5-3-1- 5的α-葡萄糖苷酶抑制活性次之(80.67%),抗氧化DPPH含量最高(15.40 μmol TE g. 1提取物)。FRAP法抗氧化性最高的是Adelina IPB的观赏辣椒。生化参数相关性显示,DPPH-AGI呈显著正相关,FRAP-AGI呈显著负相关。本研究结果可为今后观赏辣椒育种研究中功能性观赏植物的开发提供依据。
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引用次数: 2
Heteromorphic, physiological and molecular characteristics of Gazania spp grow in North Coast of Egypt 生长在埃及北海岸的Gazania spp的异型性、生理和分子特征
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2440
Eman Tawfik, M. Soliman
Abstract Gazania (L.) is one of wild ornamental plant which has special architectures. It is a small genus of 16 species, belonging to the family Asteraceae (Formerly: Compositae). It is low-growing perennial herb, grow in clumps and can also form dense carpets covering large areas. Gazania has multiple genera and species with mutli-petals colors. The chlorophyll pigmentation was measured in leaves and petals of these specimens which show high significant difference. Genomics is the study of genomes, and it can be used to conduct research on genome evolution, genomic variations, gene regulation, and important biological mechanisms using detailed genome sequence information. The diversity of ornamental plants, as well as the slow progress of genome research on ornamental plants for a long time. RAPD-PCR was applied in the present study to estimate the genetic variation in these samples which resulted in a total polymorphism percentage of 42.36%. This polymorphism percentage lead to the variation in essential physiological pathways in Gazania spp and reflected on the multivariate petals color.
摘要Gazania(L.)是一种具有特殊结构的野生观赏植物。它是菊科(原名:菊科)的一个小属,共有16种。它是生长缓慢的多年生草本植物,丛生,也可以形成覆盖大面积的密集地毯。Gazania有多个属和种,花瓣颜色各异。对叶片和花瓣的叶绿素色素含量进行了测定,结果表明,叶片和花瓣叶绿素色素含量差异显著。基因组学是对基因组的研究,它可以利用详细的基因组序列信息进行基因组进化、基因组变异、基因调控和重要生物学机制的研究。观赏植物的多样性,以及长期以来观赏植物基因组研究进展缓慢。本研究应用RAPD-PCR方法对这些样品的遗传变异进行了估计,总多态性百分比为42.36%。该多态性百分比导致了Gazania spp基本生理途径的变异,并反映在多变量花瓣颜色上。
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引用次数: 0
Physical and chemical characteristics and analysis of plant substrate 植物基质的理化特性及分析
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2496
G. Schafer, Betina Luíza Lerner
Abstract Cultivation in protected environments and containers culminated in the need of the use substrates with specific chemical and physical characteristics; assuming that a fundamental role in cultivation of horticultural plants. In this way, the objective was to describe the main physical and chemical characteristics of substrates, as well as the main methodologies for analysis. As physical characteristics we can refer as examples: density on a wet and dry basis, total porosity, air space and water retention (easily available, buffering capacity and remaining water). The most important chemical characteristics in substrates include pH, electrical conductivity and available nutrient content. These characteristics are responsible for all the nutritional dynamics and the availability of water and air in the culture medium, so they must be known, tested and managed during cultivation. Thus, it is possible to establish parameters for plant cultivation in containers, being also possible to correlate its influence on plant development for scientific research.
摘要在受保护的环境和容器中培养,最终需要使用具有特定化学和物理特性的基质;认为这是园艺植物栽培的基本作用。通过这种方式,目的是描述基质的主要物理和化学特性,以及分析的主要方法。作为物理特性,我们可以举几个例子:干湿密度、总孔隙率、空气空间和保水性(易得性、缓冲能力和剩余水)。基质中最重要的化学特性包括pH值、电导率和有效养分含量。这些特性是培养基中所有营养动态以及水和空气可用性的原因,因此必须在培养过程中了解、测试和管理这些特性。因此,可以建立容器中植物培养的参数,也可以将其对植物发育的影响联系起来进行科学研究。
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引用次数: 7
Water stress enhances geranium (Pelargonium) cuttings rooting quality 水分胁迫提高了天竺葵插枝的生根质量
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2462
Yaarit Kutsher, D. Evenor, M. Reuveni
Abstract Pelargonium nurseries in Europe encounter rooting problems due to the low quality of the cuttings supplied during the winter. The problem may be due to the poor quality of the stock plants from which the cuttings are harvested. The main problem that growers have is the prolonged shipping period and its effect on the viability and rootability of the cuttings once they arrive in Europe. We tested the effect of water stressing the stock plants grown grown in random block design in a commercial nursery and checked the rootability of cuttings after storage and the susceptibility of cuttings to rot. We assumed that mild water stress could harden the stock plant and thus the cuttings, and they would survive the journey better. Mild water stress improved the rooting ability of some Pelargonium varieties after days of storage compared to unstressed plants. Cuttings from water-stressed stock plants showed less decay after prolonged storage than unstressed stock plants as measured by percent rotten cutting. Chlorophyll content increased significantly in cuttings from stressed stock plants. The yield and physical parameters of the cuttings from stressed and nonstressed stock plants did not change. Results indicate that applying water stress to stock plants improves the quality of Pelargonium cuttings.
摘要欧洲天竺葵苗圃由于冬季供应的插枝质量不高而遇到生根问题。这个问题可能是由于采收插枝的砧木质量差。种植者面临的主要问题是运输时间过长,一旦到达欧洲,它会影响插枝的生存能力和植根性。我们在一个商业苗圃中以随机块设计的方式对砧木进行了水分胁迫的效果测试,并检查了砧木储存后的生根性和砧木腐烂的易感性。我们认为轻微的水分胁迫可以使砧木变硬,从而使砧木变硬,从而使砧木更好地存活下来。与未受水分胁迫的品种相比,轻度水分胁迫提高了部分天竺葵品种的生根能力。以腐烂扦插的百分比衡量,受水分胁迫的砧木扦插在长时间贮藏后的腐烂程度低于未受水分胁迫的砧木。受胁迫砧木扦插的叶绿素含量显著增加。受胁迫和未受胁迫的砧木扦插的产量和物理参数没有变化。结果表明,对砧木施加水分胁迫可提高天竺葵插枝的质量。
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引用次数: 0
Asymbiotic seed germination and in vitro development of orchid Papilionanthe Miss Joaquim 金凤兰种子萌发与离体发育的研究
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2431
Sachin S Patavardhan, Susan Ignatius, Rinda Thiyam, Queenie Lasrado, Sulakshana Karkala, L. D’Souza, S. Nivas
Abstract Orchids with their sheer variety of species are amazing, a major sharer in global floriculture trade. Papilionanthe Miss Joaquim, well known hybrid orchid, also recognized as official National flower of Singapore is a resilient, sun loving orchid, which blossoms throughout the year. Though a seed pod derived from a single flower contains millions of dusty seeds, due to lack of endosperm, seeds cannot germinate without the help of symbiont fungus. Commercial production of seedlings thus remains challenging. Therefore, in this study, we report a novel asymbiotic seed germination protocol standardized for ‘Papilionanthe Miss Joaquim’ (a hybrid of Papilionanthe hookeriana x Papilionanthe teres) which could be adapted for mass cultivation in a commercial setup. Seed of Papilionanthe Miss Joaquim were treated with 0.1% H2O2 and 0.1% KNO3 to increase the germination rates. Seed pods were also subjected to various regimes of surface sterilization methods to reduce contaminations. Employing Carbendazim (1% w v-1), Tricyclazole (1% w v-1), Sodium hypochlorite (0.5% v v-1) and Ethanol (80% v v-1) in surface sterilization process, resulted in highest percentage of aseptic cultures. Seeds were inoculated on to Murashige and Skoog (MS) medium supplemented with BAP (6-Benzylaminopurine), NAA (1-Naphthaleneacetic acid) and 2,4-Dichlorophenoxyacetic acid (2,4-D) combination of 3 mg L-1 BAP (6-Benzylaminopurine) + 0.5 mg L-1 NAA (1-Naphthaleneacetic acid) found to be most effective to induce germination (84.67 ± 3.2%). Subsequently, germinated seedlings were subjected to different levels of BAP and NAA levels to achieve the highest number of plantlets. thus, multiplied plantlets were later subcultured onto MS medium containing 0.5 mg L-1 BAP and 3 mg L-1 NAA to induce rooting. Consequently, developed plantlets were acclimatized on a substratum containing coconut husk and charcoal pieces.
摘要兰花种类繁多,令人惊叹,是全球花卉贸易的主要分享者。凤蝶Joaquim小姐是一种著名的杂交兰花,也是新加坡的官方国花,是一种坚韧、喜阳光的兰花,全年开花。尽管从一朵花中提取的种子荚中含有数百万粒尘封的种子,但由于缺乏胚乳,如果没有共生真菌的帮助,种子就无法发芽。因此,种苗的商业化生产仍然具有挑战性。因此,在本研究中,我们报道了一种新的无歧义种子发芽方案,该方案适用于“Papilionanthe Miss Joaquim”(凤蝶与凤蝶的杂交种),可在商业环境中大规模种植。用0.1%H2O2和0.1%KNO3处理若金凤蝶种子,提高了其发芽率。种子荚也受到各种制度的表面消毒方法,以减少污染。在表面灭菌过程中使用多菌灵(1%w v-1)、三环唑(1%w v.1)、次氯酸钠(0.5%v v-1)和乙醇(80%v v.1),可获得最高的无菌培养率。将种子接种在Murashige和Skoog(MS)培养基上,该培养基补充有BAP(6-苄基氨基嘌呤)、NAA(1-萘乙酸)和2,4-二氯苯氧基乙酸(2,4-D)组合3 mg L-1 BAP(6-Benzylamino嘌呤)+0.5 mg L-1 NAA(1-萘乙酸),对发芽的幼苗进行不同水平的BAP和NAA处理以获得最高的植株数量。因此,繁殖的植株随后在含有0.5mg L-1 BAP和3mg L-1 NAA的MS培养基上继代培养以诱导生根。因此,发育好的植株在含有椰子壳和木炭块的基质上驯化。
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引用次数: 2
Vase life consequences of natural and chemical treatments in foxtail lily (Eremurus spectabilis), as a specialty cut flowers 狐尾百合(Eremurus spectabilis)是一种特种切花,自然和化学处理对其花瓶寿命的影响
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/2447-536x.v28i1.2419
Y. Basiri, N. Etemadi, M. Alizadeh, A. Nikbakht, G. Saeidi
Abstract Eremurus spectabilis is a new, commercially valuable specialty cut flower, but little is known about the applicable treatments to extend the vase-life and maintaining the ornamental quality of this flower. Therefore, the present study was aimed at investigating the impacts of nano-silver, essential oils, and chemical treatments on keeping ornamental quality and vase-life of cut inflorescences of Foxtail lily. The cut inflorescences were placed in different vase solutions containing salicylic acid (100 and 200 mg L-1), methyl jasmonate (25 and 50 mg L-1), silver thiosulfate (0.1 mM) plus isothiazolinone (0.001 and 0.005 mL L-1), nano-silver (5, 10, and 15 mg L-1), thymol (75 and 120 mg L-1), and menthol (75 and 120 mg L-1), which were applied as continuous and pulsing methods. According to the results, all treatments considerably improved the post-harvest performance of Foxtail lily cut flowers. Although there were no significant differences among nano-silver (NS) treatments, the cut inflorescences kept in vase solution containing 10 mg L-1 of NS exhibited the longest vase life (~14 days) and the best ornamental quality. Nano-silver treatment followed by silver-thiosulfate significantly improved solution uptake by flowering inflorescences, thereby delaying the water balance loss and keeping relative fresh weight. Furthermore, 50 mg L-1 methyl jasmonate- and 75 mg L-1 menthol-treated cut inflorescences exhibited significantly higher vase-life longevity by ~ 6 and 4 days, total soluble solutes by 28.38% and 19.12%, and solution uptake rate by 76.46% and 140.6%, respectively, as compared to control. Overall, 10 mg L-1 NS can be recommended as a commercial preservative solution to delay flower senescence and improve the vase life and keeping the quality of foxtail cut inflorescence.
摘要野樱是一种新型的、具有商业价值的特色切花,但目前对延长其花瓶寿命和保持其观赏品质的处理方法知之甚少。因此,本研究旨在探讨纳米银、精油和化学处理对毛尾百合切花观赏品质和花瓶寿命的影响。将剪下的花序置于不同的花瓶溶液中,分别含有水杨酸(100和200 mg L-1)、茉莉酸甲酯(25和50 mg L-1)、硫代硫酸银(0.1 mM)加异噻唑啉酮(0.001和0.005 mL L-1)、纳米银(5、10和15 mg L-1)、百里香酚(75和120 mg L-1)和薄荷醇(75和120 mg L-1),以连续和脉冲方式施用。结果表明,各处理均能显著提高黄尾莲切花的采后性能。纳米银(NS)处理间无显著差异,但10 mg L-1纳米银溶液中切花的成瓶寿命最长(~14 d),观赏品质最好。纳米银处理后的硫代硫酸银处理显著提高了开花花序对溶液的吸收,从而延缓了水分平衡的丧失,保持了相对鲜重。此外,50 mg L-1茉莉酸甲酯和75 mg L-1薄荷醇处理的切花花期分别比对照延长了6天和4天,可溶性溶质总量分别提高了28.38%和19.12%,溶液吸收率分别提高了76.46%和140.6%。综上所述,10mg l - 1ns可作为延缓花衰老、延长花瓶寿命和保持毛缕插花质量的商业防腐剂。
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引用次数: 2
Exploration and morphologic variation of Iris wild species with ornamental potential 具有观赏潜力的鸢尾野生种的探索与形态变异
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/2447-536x.v28i1.2409
E. Asgari, M. Taghizadeh, A. Abbasifar
Abstract Nowadays, the flower industry is growing so fast that its development requires introducing new varieties to the flower market. Iran is the habitat of numerous species of wild plants that are among the valuable genetics in the breeding process. The identification of native Iris species, investigation of morphological diversity, and distribution method have a significant effect on the introduction of this flower as new and superior cultivars cut flower and bedding plant. The documentation of the most important habitats of Iris in Arak province, identification of morphological characteristics and correlation determination of morphological traits are the most important aims of this research. The results showed that there is a correlation between some of the traits. The highest positive correlation was related to the height of flower and length of the leaf, length of the flowering stem and the length of the leaf and the height of the flower and the length of the flowering stem. The ecotypes of Iris sp. were divided into six groups. Based on the results of the cluster diagram, the bulbous species were separated from rhizome species. Generally, due to the color diversity of Iris sp. and unique beauty of the flowers and the resistance of the native species, they can be used as native foundations. Due to the prominent traits such as high durability of Iris meda, odor of I. hymernospatha Subsp. leptoneura and color diversity of I. persica, I. songarica and height of I. spuria, they can be used to introduce new ornamental species. Iris spuria is the most suitable genotype because of the high height, large flower, high durability, and beautiful flower.
摘要如今,花卉产业发展如此之快,其发展需要向花卉市场引进新品种。伊朗是许多野生植物的栖息地,这些野生植物是繁殖过程中有价值的遗传学之一。本地鸢尾属植物种类的鉴定、形态多样性的调查和分布方法对引进该花作为新的优良品种、切花和床上用品具有重要影响。对阿拉克省鸢尾最重要的栖息地进行记录、形态特征鉴定和形态特征相关性测定是本研究的重要目的。结果表明,某些性状之间存在相关性。最高的正相关性与花的高度和叶的长度、花茎的长度和叶的高度以及花茎的高度有关。将鸢尾属的生态型分为6组。根据聚类图的结果,从根茎类植物中分离出球茎类植物。一般来说,由于鸢尾属植物的颜色多样性、花朵的独特美丽以及本地物种的抗性,它们可以用作本地基础。由于鸢尾花具有高耐久性等突出特点。钩端神经的遗传多样性和毛白杨的颜色多样性,以及毛白杨的高度,可作为引种新的观赏树种。花高、花大、耐久性强、花漂亮,是最适合的基因型。
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引用次数: 1
Response of black mulberry onto white mulberry rootstock to stenting (cutting-grafting) techniques and IBA concentrations 黑桑对白桑砧木扦插技术和IBA浓度的响应
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/2447-536x.v28i1.2413
M. Solgi, M. Taghizadeh, H. Bagheri
Abstract Ornamental black mulberry (Morus nigra L.) is used in landscape. Ornamental black mulberry which has high shade, tolerates air pollution and wind. White mulberry (Morus alba L.) is proper for kind of soils conditions. Therefore, this research was conducted to investigate the effects of stenting methods and IBA levels on some characters of black mulberry scions onto white mulberry rootstock for the first time. For this purpose, an experiment was carried out as factorial in completely randomized design with 10 replications. Two factors were including two stenting method (splice and omega) and three levels of Indole-3-butyric acid (0, 500 and 1000 mg L-1). Three months after grafting, percentage of rootstock callus formation, percentage of rooting, percentage of leaf formation, number of leaf formation, the longest shoot and the longest root formation were measured. Results showed that the effect of stenting method was significant on all of evaluated parameters and splice method was superior to omega method. Also, the effects of different levels of IBA were significant on all of measured traits and they are increased by increasing the levels of IBA and the best was with 1000 mg L-1. The interaction effects of these factors are significant on all of measured characteristics except for percentage of rootstock callus formation and rooting percentage. In conclusion, the stenting via splice method had higher success in comparison with omega method and the morphological traits increased by increasing the application of higher levels of IBA. The application of stenting method by splice plus 1000 mg L-1 IBA which was carried out for the first time is recommended for reproduction of ornamental black mulberry onto white mulberry.
摘要:观赏桑树(Morus nigra L.)是一种园林绿化植物。观赏黑桑树,树阴高,耐空气污染和风。白桑树(Morus alba L.)适合于某些土壤条件。因此,本试验首次研究了不同支架植入方式和IBA水平对黑桑接穗在白桑砧木上部分性状的影响。为此,采用10个重复的完全随机设计,进行因子试验。两个因素包括两种支架置入方式(splice和omega)和吲哚-3-丁酸(0,500和1000 mg L-1)的三个水平。嫁接3个月后,测定砧木愈伤组织形成率、生根率、成叶率、成叶数、最长芽和最长根形成率。结果表明,支架植入法对各评价指标的影响均显著,且剪接法优于omega法。不同水平IBA对各性状的影响均显著,且随IBA水平的增加而增加,以1000 mg L-1处理效果最好。除砧木愈伤组织形成率和生根率外,这些因素的互作效应对其他性状均有显著影响。综上所述,与omega法相比,splice法支架置入术的成功率更高,并且随着IBA用量的增加,支架的形态性状也有所改善。建议首次采用剪接支架法加1000 mg L-1 IBA进行观赏黑桑在白桑上的繁殖。
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引用次数: 3
Overcoming dormancy in seeds of Dietes bicolor (Steud.) Sweet ex Klatt 植物种子克服休眠的研究可爱的前女友克拉特
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/2447-536x.v28i1.2410
Thiago Roberto Rezende Borges, Maurecilne Lemes da Silva, G. Silva, D. I. Rocha
Abstract Seminiferous propagation of Dietes bicolor is hindered by the probable physical and/or morphophysiological dormancy. The objective was to analyze the efficacy of different methods of overcoming dormancy in D. bicolor seeds to determine a possible pre-germination treatment for the species. Two experiments were conducted: (I) Evaluation of the breaking of physical dormancy, in which the treatments of mechanical scarification were carried using sandpaper #100; chemical scarification with sulfuric acid (H2SO4) for 5 and 10 minutes and immersion in hot water (70 ºC), also, for 5 and 10 minutes, were evaluated. The intact seed was considered a control. (II) Evaluation of morphophysiological dormancy, in which the seeds were submitted to combined treatments of stratification of hot temperature (20-35 °C) and/or cold temperature (9 ºC), distributed in periods of 0, 1, and 2 weeks, totalizing 9 treatments. In both experiments, germination percentage (G), germination speed index (GSI), and first count (FC) were determined, using a randomized block design, and evaluated by the Scott-Knott test at 1% and Dunnett’s test at 5%. The use of H2SO4 10 minutes induced 42% germination, 0.52 GSI, and 18% FC, but only FC was significantly different from the control. In the second trial, the treatment that spent only two weeks in hot temperatures (20-35 ºC) showed the highest germination (30%), compared to the control (G = 22%). These results provide relevant information for understanding the physiology of D. bicolor germination, in addition to contributing to the optimization of pre-germination practices for this important ornamental species.
摘要:双色Dietes的精生繁殖可能受到生理和/或形态生理休眠的阻碍。目的是分析不同方法克服双色天葵种子休眠的效果,以确定该物种可能的发芽前处理方法。进行了两项试验:(1)物理休眠打破评价,其中机械划伤处理采用100号砂纸进行;用硫酸(H2SO4)化学刻蚀5和10分钟,热水(70ºC)浸泡5和10分钟,也进行了评估。完整的种子作为对照。(二)形态生理休眠评价,采用高温(20 ~ 35℃)和低温(9℃)分层组合处理,分0、1、2周进行,共9个处理。在两个试验中,采用随机区组设计测定发芽率(G)、发芽速度指数(GSI)和首次计数(FC),并采用1%的Scott-Knott检验和5%的Dunnett检验进行评价。H2SO4处理10分钟萌发率为42%,GSI为0.52,FC为18%,但只有FC与对照有显著差异。在第二次试验中,与对照(G = 22%)相比,仅在高温(20-35ºC)下度过两周的处理显示出最高的发芽率(30%)。这些结果为了解双色花的萌发生理学提供了相关信息,并有助于优化这一重要观赏植物的萌发前措施。
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引用次数: 0
Extending vase life of carnation flowers by postharvest nano silver, humic acid and Aloe Vera gel treatments 纳米银、腐殖酸和芦荟凝胶处理延长康乃馨花瓶寿命
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/2447-536x.v28i1.2407
A. B. El-Attar, W. R. Sakr
Abstract Standard Carnation flowers (Dianthus caryophyllus cv. Dover) were harvested at the paint brush stage in the early morning, pre-cooled at 4° C for 6-h then moved under dry conditions to the laboratory. Flowers were weighted and treated for 24-h with silver nanoparticles (AgNPs) at 0, 5 or 10 ppm in plastic buckets. After pulsing treatments, cut flowers were transferred to 500 mL glass jars containing 300 mL of preservation solution treatments including, individually, humic acid at 200, 400 or 600 ppm and Aloe vera gel at 2.5%, 5.0% or 7.5% (w/v) till the end of the experiment (when flower color began to fade, petals began to roll). Distilled water was used for the control and to prepare the tested solutions. 2% sucrose was added to all treatments including the control. Addition of all preservatives to vase solutions significantly increased all the studied characteristics of cut flowers compared to control (distilled water, least values). Nanosilver treatments have the potential to be used as preservative solutions for improving postharvest vase life and quality of carnation flowers. An increment in vase life, relative fresh weight, vase solution uptake, flower diameter as well as total chlorophylls in leaves, total carbohydrates and total phenols in leaves and petals was observed, in addition to a decrease in bacterial counts in vase solution. Best results were obtained using 5 ppm AgNPs + 5.0% Aloe vera gel followed by 5 ppm AgNPs +7.5% Aloe vera gel solutions. Aloe vera gel, especially 5.0% level, showed better results than humic acid when used alone or combined with AgNPs. Nanosilver at 5 ppm recorded better results than 10 ppm when used as a single treatment.
标准石竹花(Dianthus caryophyllus cv.;多佛)在清晨刷漆阶段收获,在4°C预冷6小时,然后在干燥条件下移至实验室。花称重,并处理24小时与银纳米粒子(AgNPs)在0,5或10 ppm的塑料桶。脉冲处理后,切花被转移到500 mL的玻璃罐中,其中含有300 mL的保存溶液,分别包括200,400或600ppm的腐植酸和2.5%,5.0%或7.5% (w/v)的芦荟凝胶,直到实验结束(当花的颜色开始褪色,花瓣开始滚动)。用蒸馏水作为对照,制备被试溶液。包括对照组在内的所有处理均添加2%蔗糖。与对照(蒸馏水,最小值)相比,在花瓶溶液中添加所有防腐剂显著增加了切花的所有研究特征。纳米银处理有潜力作为保鲜溶液用于改善康乃馨的采后花瓶寿命和质量。瓶内细菌数量减少,瓶内寿命、相对鲜重、瓶液吸收量、花径、叶片总叶绿素、叶片和花瓣总碳水化合物和总酚含量增加。使用5 ppm AgNPs + 5.0%芦荟凝胶溶液获得最佳效果,其次是5 ppm AgNPs +7.5%芦荟凝胶溶液。芦荟凝胶在单独使用或与AgNPs联合使用时,以5.0%水平的芦荟凝胶效果优于腐植酸。纳米银浓度为5ppm时,单次处理的效果优于10ppm。
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引用次数: 6
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Ornamental Horticulture
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