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A review: use of soilless culture techniques in ornamental plants 无土栽培技术在观赏植物中的应用综述
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536X.v28i2.2430
F. P. Karagöz, A. Dursun, Melek Karaşal
Abstract The use of soilless culture in the cultivation of ornamental plants is becoming widespread, especially in the production of cut flowers. The aim of this review is to present a brief summary of both standard and new findings obtained from using soilless culture techniques in ornamental plant cultivation, based on a detailed literature review. In the researches reached for this review, Calendula officinalis L., Matthiola incana L., Pelargonium zonale L., Petunia x hybrita L., Pinus pinea, Cupressus arizonica, C. sempervirens, Sternbergia lutea, Galanthus elwesii Hook, Lilium candidum, Limonium sinuatum, Eustoma grandiflorum (Lisianthusrussellianus) and Tulipa gesneriana species were evaluated as plant material. In the researches evaluated, different ornamental plant species, soilless culture techniques and substrates of soilless culture were examined and important results were obtained. Moreover, in the changing living conditions with COVID-19, people have grown plants in their gardens, balconies, terraces and even living rooms using simple hydroponic systems. Thus, they made the simplest practices of soilless culture and even brought it into their homes. It can be foreseen that the commercial and scientific use of hydroponic culture systems in ornamental plants will continue and rapidly increase.
摘要无土栽培在观赏植物栽培中的应用越来越广泛,尤其是在切花生产中。本综述的目的是在详细文献综述的基础上,简要总结无土栽培技术在观赏植物栽培中的标准和新发现。在本综述的研究中,对金盏花、马齿苋、天竺葵、矮牵牛、松树、亚利桑那柏、三叶草、木犀、金花莲、白百合、血草、桔梗和苦苣苔进行了植物材料评价。在评价的研究中,对不同的观赏植物种类、无土栽培技术和无土栽培基质进行了研究,取得了重要的成果。此外,在新冠肺炎不断变化的生活条件下,人们在花园、阳台、露台甚至客厅里使用简单的水培系统种植植物。因此,他们对无土文化进行了最简单的实践,甚至将其带进了自己的家中。可以预见,水培栽培系统在观赏植物中的商业和科学应用将继续并迅速增加。
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引用次数: 6
Grape-based residue as a substrate in Oncidium baueri Lindl. acclimatization 葡萄基残渣作为球莲底物的研究。适应环境
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2477
M. Nadal, A. M. Assis, M. Schuch, R. T. Faria
Abstract The use of agricultural residues as a substrate is a promising option for orchid acclimatization, which is a critical stage of micropropagation. Thus, this study aimed to evaluate the use of grape residue S-10 Beifort®, isolated or mixed with other agricultural residues, in the acclimatization of Oncidium baueri Lindl. Five treatments were studied (S-10 Beifort®; S-10 Beifort® + carbonized rice husk (CRH); S-10 Beifort® + coconut fiber (CF); S-10 Beifort® + CRH + CF; CRH + CF), with four replications containing 10 seedlings each. After 120 days, the highest survival rate occurred with the mixture CRH + CF (62.5%). The mixture of S-10 Beifort® with CRH provided a greater shoot length (3.7 cm), not differing from the mixture of the three substrates (2.9 cm). S-10 Beifort® + CRH and the mixture of the three substrates ensured greater root length (3.8 cm). The mixture of the three residues provided the highest average of fresh and dry mass accumulation. The pH variation between the substrates was from 5.2 to 6.3, while S-10 Beifort® presented the highest electrical conductivity (2030 μS cm-1) and the mixture of this material with carbonized rice husk presented the highest water retention capacity. The use of a mixture of S-10 Beifort® with carbonized rice husk or with carbonized rice husk and coconut fiber (1:1:1) is indicated for the acclimatization of Oncidium baueri Lindl.
摘要利用农业残留物作为基质是兰科植物驯化的一种很有前途的选择,这是兰科植物微繁的关键阶段。因此,本研究旨在评价葡萄渣S-10 Beifort®分离或与其他农业残留物混合在蓝球莲(Oncidium baueri Lindl)驯化中的应用。研究了5种处理(S-10 Beifort®;S-10 Beifort®+碳化稻壳(CRH);S-10 Beifort®+椰子纤维(CF);S-10 Beifort®+ CRH + CF;CRH + CF), 4个重复,每个重复10株苗。120 d后,CRH + CF的存活率最高(62.5%)。S-10 Beifort®与CRH的混合物提供了更大的茎长(3.7 cm),与三种底物的混合物(2.9 cm)没有区别。S-10 Beifort®+ CRH和三种基质的混合物确保了更长的根长(3.8 cm)。三种残留物的混合物提供了最高的平均鲜质量和干质量积累。不同基质的pH值变化范围为5.2 ~ 6.3,其中S-10 Beifort®的电导率最高(2030 μS cm-1),该材料与碳化稻壳的混合物具有最高的保水能力。使用S-10 Beifort®与碳化稻壳或碳化稻壳与椰子纤维(1:1:1)的混合物用于蓝心球茎的驯化。
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引用次数: 1
Amino acid solutions on the growth of the ornamental plant Impatiens walleriana grown under root restriction stress 氨基酸溶液对观赏植物凤仙花根限制胁迫下生长的影响
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2439
Guido Hakim, Esteban Gandolfo, Máximo Salinas, E. Giardina, A. Benedetto
Abstract The use of amino acid solutions as bio-fertilizers was introduced in the food and ornamental plant industry about two decades ago. Sprays containing amino acids either alone or combined have been proven effective to increase plant growth. However, many of the new commercial solutions including amino acids as bio-fertilizers have no published data available on bedding plants. Thus, the aims of this study were to determine the changes in fresh weight accumulation in the bedding pot plant Impatiens walleriana and the physiological mechanism involved in plants sprayed with different amino acid solutions and to characterize the responses to root restrictions under nursery with non-limited nitrogen supply. The experiment was carried out inside a greenhouse in the campus of the Faculty of Agronomy of the University of Buenos Aires, Argentina. To reach the objectives, Impatiens walleriana ‘Xtreme White’ seeds were grown in 50-cell (55.7 cm3 cell-1) and 288-cell (6.18 cm3 cell-1) plug trays and then transplanted and grown at 3-L pots. Eleven solutions (100 mg L-1) containing an equal amount of each of the following amino acids: alanine, cysteine, aspartic acid, glutamic acid, glycine, leucine, lysine, methionine, threonine, tryptophan and valine, a control without amino acids, and a solution containing all amino acids (Mix) (9.1 mg L-1 of each amino acid) were sprayed when the first true leaf pair was developed. Our results showed that the different synthetic amino acids tested, alone or combined, had additive and antagonistic effects on the leaf area and fresh-dry weight in Impatiens walleriana plants.
摘要大约20年前,氨基酸溶液作为生物肥料被引入食品和观赏植物行业。单独或联合使用含有氨基酸的喷雾已被证明对促进植物生长有效。然而,许多新的商业解决方案,包括氨基酸作为生物肥料,都没有关于床上用品的公开数据。因此,本研究的目的是确定垫盆植物凤仙花鲜重积累的变化以及不同氨基酸溶液对植物的生理机制,并表征在无限制氮供应的苗圃下对根系限制的反应。实验在阿根廷布宜诺斯艾利斯大学农学学院的温室内进行。为了达到目的,将凤仙花“Xtreme White”种子在50个细胞(55.7 cm3 cell-1)和288个细胞(6.18 cm3 cell-1。当第一对真叶发育时,喷洒11种溶液(100 mg L-1),其中含有等量的下列氨基酸:丙氨酸、半胱氨酸、天冬氨酸、谷氨酸、甘氨酸、亮氨酸、赖氨酸、甲硫氨酸、苏氨酸、色氨酸和缬氨酸,一种不含氨基酸的对照,以及一种含有所有氨基酸的溶液(混合物)(每种氨基酸9.1 mg L-1。我们的结果表明,测试的不同合成氨基酸,单独或组合,对凤仙花植物的叶面积和鲜干重具有相加和拮抗作用。
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引用次数: 0
Semihydroponic and ebb-and-flow systems for calla lily cultivation 马蹄莲半水培和潮落栽培系统
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2422
Karina Volpi Furtini Boldrin, P. Paiva, J.V.C. Barbosa, Sabrina dos Santos Torquetti, E. Almeida, Simone Novaes Reis, P. R. Landgraf
Abstract The soilless cultivation of plants is an alternative for the production of flowers with high quality. Calla lily is normally produced on soil benches, but have shown some limitations as bacterial infections occur. One alternative may be a soilless cultivation. Hence, experiments were conducted with the objective to evaluate the development of calla lily in two types of hydroponic production systems. The semihydroponic system was tested using as substrates or growing media such as, coconut fiber, sand, vermiculite, and phenolic foam sheets. For the other system, ebb-and-flow, the substrates tested were vermiculite, coconut fiber, and expanded clay. Two different nutritional solutions were tested in both systems. in the semihydroponic system. In general, calla lily was adapted and showed a good development in a soilless cultivation. The best initial development of the calla lily was provided using vermiculite and sand as substrates on semihydroponic. And for the ebb-and-flow system, coconut fiber induced better development. As conclusion, hydroponic cultivation systems are efficient for the initial development of calla lily. The recommended substrates are those with lower porosity, such as sand, vermiculite, and coconut fiber.
摘要植物无土栽培是生产高品质花卉的一种替代方法。马蹄莲通常在土壤台架上生产,但由于细菌感染,已经显示出一些局限性。一种选择可能是无土栽培。因此,进行了实验,目的是评估马蹄莲在两种水培生产系统中的发育。使用椰子纤维、沙子、蛭石和酚醛泡沫片作为基质或生长介质来测试半水培系统。对于另一个系统,潮落,测试的基质是蛭石、椰子纤维和膨胀粘土。在两个系统中测试了两种不同的营养溶液。在半水培系统中。总的来说,马蹄莲在无土栽培中得到了很好的适应和发展。以蛭石和沙子为基质,在半水培条件下,为马蹄莲的早期发育提供了最佳条件。对于潮落系统,椰子纤维诱导了更好的发育。总之,水培栽培系统对马蹄莲的初步发育是有效的。推荐的基质是孔隙率较低的基质,如沙子、蛭石和椰子纤维。
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引用次数: 1
Development and nutritional status of calla lily submitted to nutrient deficiency 营养不良条件下马蹄莲的发育及营养状况
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2476
E. Almeida, P. Paiva, Jussara Ellen Morais Frazão, M. N. O. Ribeiro, Nilma Portela Oliveira
Abstract Knowledge about the nutritional aspects of calla lily, an important species for the floriculture industry, is essential for quality on cultivation. As there are variations in the symptoms of nutritional deficiencies among species, it is necessary to study the development of plants and their nutritional status when grown under nutrient omission. Thus, the aim was to evaluate the development of calla lily plants and identify the levels of nutrients and their interactions in cultivation conditions under nutritional deficiency. The treatments consisted in the use of the complete nutrition solution and the omission of N, P, K, Ca, Mg, S, Fe, B, and Mn, separately. The experimental design was in randomized block design with four replications. The omissions of P, S, Ca, K, N, and B have influenced the plant development providing lower production of leaves and in small size, reduction on shoots production and less leaves emitted by the shoots, besides a lower values of dry matter. Among these nutrients, the deficiencies that most affected the growth and quality of calla lily plants development were N, B and Ca. The B and N deficiency inhibited the flowering and the absence of S and K provided inflorescences production with lower quality, besides malformation. The main interactions that occurred were increasing in the content of K (leaves), Fe (leaves) and Mn (leaves and inflorescences) in the absence of Ca. In the absence of Mg there was an increase in Ca (leaves, rhizomes, and inflorescences), Zn (leaves and roots) and Fe (rhizomes). It can be concluded that the nutritional deficiencies that most affected the production of inflorescences in calla lily were those of B, N, P, K, Ca, and S and the main nutritional interactions occurred in the absence of Ca with an increase in K, Fe and Mn and an increase in Ca, Fe and Zn in the absence of Mg.
摘要马蹄莲是花卉栽培的重要品种,了解其营养成分对提高马蹄莲的栽培质量至关重要。由于营养缺乏的症状在不同物种之间存在差异,因此有必要研究植物在营养缺失条件下的发育及其营养状况。因此,目的是评价马蹄莲植株的发育,确定营养缺乏栽培条件下的营养水平及其相互作用。处理包括使用全营养液和分别不施用N、P、K、Ca、Mg、S、Fe、B和Mn。试验设计为随机区组设计,设4个重复。P、S、Ca、K、N和B的遗漏影响了植株的发育,导致叶片产量降低,且在较小的尺寸下,梢产量减少,梢排叶量减少,干物质值降低。其中,对马蹄莲植株发育影响最大的是N、B和Ca。B和N的缺乏抑制了开花,S和K的缺乏导致花序质量下降,并造成畸形。在缺乏Ca的情况下,钾(叶)、铁(叶)和锰(叶和花序)含量增加,而在缺乏Mg的情况下,钙(叶、根和花序)、锌(叶和根)和铁(根)含量增加。综上所述,对马蹄莲花序生长影响最大的营养缺乏是B、N、P、K、Ca和S,主要的营养相互作用发生在缺Ca时,K、Fe和Mn增加,缺Mg时Ca、Fe和Zn增加。
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引用次数: 1
Plant substrate 植物基质
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2459
G. Schafer
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引用次数: 1
Different storage temperatures and times on pollen quality in cut rose varieties 不同贮藏温度和贮藏时间对切花玫瑰花粉品质的影响
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2470
Selin Seyhan Korkut, S. Kazaz, T. Kılıç
Abstract Pollen parent is one of the most important factors affecting the seed set in conventional breeding. Pollen quality of pollen parents must be known for success in breeding programs. Breeders also must know how long pollen grains keep their viability to overcome geographical distance and the difference in blooming times among parents. This study was conducted to find out the viability, germination rate, and duration of the keeping viability of pollen of rose varieties being kept for 0, 4, 8, 16, 24 hours at 24 ºC and 0, 1, 2, 3, 4, 5 days at 4 ºC. The pollen of the Inferno, Layla, First Red, and Myrna varieties were used as plant material. The IKI and petri dishes methods were used to determine pollen quality. The results showed that the viable pollen rate of varieties varied between 41.1% and 49.9%, whereas the germination rate was 3.8% and 29.9% and morphological pollen rate was 71.8% and 88.7%. In all varieties, viability, germination rate and morphological normal pollen rate decreased over time both kept at 24 ºC and 4 ºC, but fresh pollen lost its quality faster than pollen stored. Fresh pollen viability rate decreased by 11.9% and 25.6% at the end of 24 hours, whereas only it decreased by 10.4%-22.6% on 1st day of storage. The reduction in germination ability in Layla, Inferno and Myrna was over 60.0% on the 5th day, while it was found less than 50.0% in First Red. The decrease in morphologically normal pollen ratio was found statistically significant in both temperature treatments, except for Layla. As it is clear, the pollen quality was significantly affected by variety, storage/holding time, and conditions. It’s recommended to use stored pollen in breeding programs. Although it varies according to the varieties, the rose pollen should be use by keeping at 4 ºC between 2-5 days.
摘要花粉亲本是影响常规育种结实率的重要因素之一。花粉亲本的花粉质量必须为育种计划的成功所知。育种者还必须知道花粉粒能保持多久的活力,以克服地理距离和父母开花时间的差异。本研究旨在了解在24ºC下保存0、4、8、16、24小时和在4ºC下保持0、1、2、3、4、5天的玫瑰品种花粉的活力、发芽率和保持活力的持续时间。使用Inferno、Layla、First Red和Myrna品种的花粉作为植物材料。采用IKI法和培养皿法测定花粉质量。结果表明,不同品种的花粉活力率在41.1%至49.9%之间,发芽率在3.8%至29.9%之间,形态花粉率在71.8%至88.7%之间。所有品种在24ºC和4ºC下的花粉活力、发芽率和形态正常花粉率都随时间下降,但新鲜花粉的质量下降速度快于储存花粉。新鲜花粉活力在贮藏24小时后分别下降11.9%和25.6%,而贮藏第1天仅下降10.4%-22.6%。第5天,Layla、Inferno和Myrna的发芽率降低了60.0%以上,而First Red的发芽率低于50.0%。除Layla外,两种温度处理的花粉形态正常率都有统计学意义。很明显,花粉质量受到品种、贮藏/保存时间和条件的显著影响。建议在育种计划中使用储存的花粉。尽管玫瑰花粉因品种而异,但应在4ºC下保存2-5天才能使用。
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引用次数: 1
Performance of native species in urban afforestation of public pathways in Lavras-MG, Brazil 巴西Lavras MG城市公共道路绿化中本地物种的表现
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2408
P. Paiva, Michele Valquíria dos Reis, R. B. Sousa, Raiy Magalhães Ferraz, M. Salgado
Abstract In addition to creating scenic landscapes and beautifying urban areas, the cultivation of trees in urban areas can benefit ecosystems and improve cities sustainability. Besides some indications of adequate species for use in public pathways, there are few studies and knowledge regarding the native species behavior and uses for this purpose. In this way, the objective of this study was to evaluate the performance of some native species in urban afforestation of public pathways. Species to be evaluated were selected considering characteristics such as occurrence (biome and region), height (medium or high size), growth rate (moderate to fast), and flowering (season and color) and at the end, 11 species were selected. The species Senna macranthera, Andira fraxinifolia, Pterocarpus violaceus, Cordia superba, Jacaranda cuspidifolia, Peltophorum dubium, Inga vera, Inga edulis, Senna multijuga, Handroanthus impetiginosus and Stifftia chrysantha were planted on a public pathway at Lavras-MG, Brazil (Biome: Mata Atlântica) and evaluated observing the initial survival, growth rate, canopy, diameter at breast height (DBH) and occurrence of damage and disease. Concerning the tested species, A. fraxinifolia and S. macranthera exhibited low survival after planting (10 months) and must be replaced for other species. Handroanthus impetiginosus and P. violaceus exhibited the best performance, presenting a good growth rate, followed by J. cuspidifolia, I. vera, and I. edulis.
摘要除了创造景观和美化城市地区外,在城市地区种植树木还可以有益于生态系统,提高城市的可持续性。除了一些迹象表明有足够的物种用于公共道路外,很少有关于本地物种行为和用途的研究和知识。通过这种方式,本研究的目的是评估一些本土物种在城市公共道路绿化中的表现。根据发生率(生物群落和区域)、高度(中等或高体型)、生长速度(中等至快速)和开花(季节和颜色)等特征选择待评估的物种,最后选择了11个物种。在巴西Lavras MG的一条公共道路上种植了品种Senna macranthera、Andira fraxinfolia、Pterocarpus violaceus、Cordia superba、蓝花兰、Peltophorum dubium、Inga vera、Inga edulis、Senna multijuga、,乳高直径(DBH)和损伤和疾病的发生。关于试验物种,A.fraxinifolia和S.macranthera在种植后(10个月)表现出较低的存活率,必须更换其他物种。脓疱草和紫丁香表现最好,具有良好的生长速度,其次是尖叶、vera和edulis。
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引用次数: 1
Initial growth of zinnia seedlings in substrate with different proportions of biosolid 不同比例生物固体基质对百日草幼苗生长的影响
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2482
Antonio Maricélio Borges de Souza, G. R. Vieira, Giovana Sgobbe, Kássia Barros Ferreira, T. Campos, K. F. L. Pivetta
Abstract Zinnia (Zinnia elegans Jacq.) is a widely cultivated species and used as an ornamental plant in the world flower market. Although it has great potential for phytoremediation and compounds with anti-infective and antioxidant activities there are few studies that address the process of production of its seedlings especially using substrates from organic waste. This study aimed to evaluate the potential of biosolid as a substrate component in the initial growth of Zinnia elegans seedlings. The seedlings were produced in nursery conditions with 50% light control. The experimental design was entirely randomized. There were six treatments, four repetitions and five plants per plot. The treatments consisted of substrates resulting from the mixture of subsoil (SS) and biosolid (BIO) in different proportions: 100% SS (control); 80% SS + 20% BIO; 60% SS + 40% BIO; 40% SS + 60% BIO; 20% SS + 80% BIO; T6 100% BIO. The morphological and quality characteristics of the seedlings were evaluated. Polynomial regression analysis and Pearson correlation coefficient were performed. Quadratic regression fitting was observed for plant height, stem diameter, leaf area, total chlorophyll, and shoot and root dry mass, as well as total dry mass. The shoot height/stem diameter ratio was the characteristic that correlated least with the others, being observed a positive correlation only with plant height. The use of biosolid in the proportion of 60% in the substrate composition proved to be efficient for the initial growth of seedlings as it showed the best responses for most morphological and quality characteristics of the seedlings.
摘要百日菊(Zinnia elegans Jacq.)是世界花卉市场上广泛栽培的一种观赏植物。尽管它在植物修复和具有抗感染和抗氧化活性的化合物方面具有巨大的潜力,但很少有研究涉及其幼苗的生产过程,尤其是使用有机废物中的基质。本研究旨在评估生物固体作为基质成分在秀丽隐杆菌幼苗初始生长中的潜力。幼苗是在50%光照控制的苗圃条件下生产的。实验设计完全随机。共有6个处理,4个重复,每个小区5株。处理由不同比例的底土(SS)和生物固体(BIO)的混合物产生的基质组成:100%SS(对照);80%SS+20%BIO;60%SS+40%BIO;40%SS+60%BIO;20%SS+80%BIO;T6 100%BIO。对幼苗的形态和质量特征进行了评价。进行多项式回归分析和Pearson相关系数。株高、茎径、叶面积、总叶绿素、地上部和根部干物质以及总干物质均呈二次回归拟合,地上部/茎径比是与其他性状相关性最小的特征,仅与株高呈正相关。在基质组合物中使用60%比例的生物固体被证明对幼苗的初始生长是有效的,因为它对幼苗的大多数形态和质量特征表现出最佳的反应。
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引用次数: 0
Seed pretreatment for control of powdery mildew infection on purple ipe micropropagation 种子预处理防治紫管微繁殖中的白粉菌感染
IF 0.7 Q3 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.1590/2447-536x.v28i2.2360
Talita Cristina Mamedes, A. A. Chaibub, K. C. I. Sousa, M. T. Faria, Letícia de Almeida Gonçalves, M. C. Filippi, L. G. Araújo, S. T. Sibov
Abstract Purple ipe (Handroanthus impetiginosus) is an important tree species in Cerrado biome conservation and very popular at the landscaping and urban afforestation. However, its micropropagation is affected by pathogens, such as Oidium sp. The aim this study was evaluate the efficiency of seed treatments in the control of powdery mildew of purple ipe obtained by micropropagation. The symptoms were observed during in vitro germination, a Koch’s postulates were performed for confirm the pathogenicity, colonization of the pathogen on the leaves was analyzed in optical and scanning microscopes and a scale to evaluate severity was proposed. Two experiments were realized to powdery mildew control using a completely randomized design, with 30 replicates. First experiment: Seeds were treated with ethanol (Et), chlorothalonil + thiophanate-methyl (C+TM), and sodium hypochlorite (NaOCl); second experiment: Seeds were treated with Et, NaOCl, C+TM, and neem oil. Disease severity and area under the disease progress curve (AUDPC) were assessed in both experiments. Disease symptoms and typical pathogen structures were observed, and the pathogenicity was confirmed. The disease severity was reduced by 30.78% in 1.5% neem oil for 10 min when compared with C+TM for 15 min. We conclude that neem oil can be a strategy sustainable for the control of powdery mildew in purple ipe in tissue culture.
紫ipe (Handroanthus impetiginosus)是塞拉多地区重要的生物群落保护树种,在园林绿化和城市绿化中非常受欢迎。但其微繁易受病原菌的影响,如Oidium sp.。本研究的目的是评价种子处理对紫ipe微繁获得的白粉病的防治效果。在离体萌发过程中观察病原菌的症状,采用科赫定律确定病原菌的致病性,在光学显微镜和扫描显微镜下分析病原菌在叶片上的定殖情况,并提出了病原菌严重程度的评分标准。采用完全随机设计进行2个白粉病防治试验,共30个重复。第一个实验:用乙醇(Et)、百菌清+甲基硫代盐(C+TM)和次氯酸钠(NaOCl)处理种子;第二个实验:用Et、NaOCl、C+TM和楝油处理种子。在两个实验中都评估了疾病严重程度和疾病进展曲线下面积(AUDPC)。观察疾病症状和典型病原菌结构,确定致病性。与C+TM处理15 min相比,1.5%印楝油处理10 min可使紫豆白粉病的严重程度降低30.78%。由此可见,在紫豆组织培养中,印楝油是一种可持续的防治策略。
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Ornamental Horticulture
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