Pub Date : 2024-08-24DOI: 10.1016/j.pathol.2024.06.009
Ryanbi Pratama , Peter C. Taylor , Chinmoy Mukerjee , Christopher J. McIver
{"title":"Mutations of cysB in urinary isolates of cysteine-requiring Escherichia coli","authors":"Ryanbi Pratama , Peter C. Taylor , Chinmoy Mukerjee , Christopher J. McIver","doi":"10.1016/j.pathol.2024.06.009","DOIUrl":"10.1016/j.pathol.2024.06.009","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":"56 7","pages":"Pages 1044-1046"},"PeriodicalIF":3.6,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142205226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-24DOI: 10.1016/j.pathol.2024.06.010
Gideon Ze Lin Tan , Jian Yuan Goh , Clarence Jia Jun Yen , Mark Edward Puhaindran , Yingting Mok
{"title":"Ossifying fibromyxoid tumour with fibrosarcoma-like features and novel PHF1::HCFC1 gene fusion","authors":"Gideon Ze Lin Tan , Jian Yuan Goh , Clarence Jia Jun Yen , Mark Edward Puhaindran , Yingting Mok","doi":"10.1016/j.pathol.2024.06.010","DOIUrl":"10.1016/j.pathol.2024.06.010","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":"56 7","pages":"Pages 1061-1063"},"PeriodicalIF":3.6,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142205106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-22DOI: 10.1016/j.pathol.2024.06.006
Ji-Seon Jeong, Uiree Jo, Gyuheon Choi, Halim Song, Kyung-Ja Cho, Joon Seon Song
Programmed cell death-ligand 1 (PD-L1) expression is a predictive biomarker for response to immune checkpoint inhibitor in head and neck squamous cell carcinoma. Given the range of antibodies and platforms for PD-L1 testing, it is essential to understand the performance of different staining and scoring methods. PD-L1 expression in 156 head and neck mucosal squamous cell carcinoma (HNmSCC) cases at Asan Medical Center was assessed using 106 tissue microarray (TMA) cores and 50 whole slides. Three standardised PD-L1 assays (22C3 pharmDx, SP263, and 28-8 pharmDx) and one laboratory-developed test (22C3 LDT) were evaluated: the combined positive score (CPS) with ≥1, ≥20, and ≥50 cut-offs, and the tumour positive score (TPS) with ≥1%, ≥20%, ≥50% cut-offs. Concordance on a continuous scale among the assays was good to excellent for CPS [intraclass correlation coefficient (ICC) range 0.73–0.94] and TPS (ICC range 0.70–0.94) and in both TMA and whole slides cohorts. Stratification by variable cut-offs demonstrated moderate to good agreement among most assays, as analysed by Gwet's AC1. PD-L1 expression was significantly correlated with tumour location using the 22C3 pharmDx assay (CPS, p=0.014; TPS, p=0.033). Notable concordance was found among PD-L1 assays, suggesting their potential interchangeability in HNmSCC.
{"title":"Comparison of PD-L1 assays in head and neck carcinoma","authors":"Ji-Seon Jeong, Uiree Jo, Gyuheon Choi, Halim Song, Kyung-Ja Cho, Joon Seon Song","doi":"10.1016/j.pathol.2024.06.006","DOIUrl":"10.1016/j.pathol.2024.06.006","url":null,"abstract":"<div><div>Programmed cell death-ligand 1 (PD-L1) expression is a predictive biomarker for response to immune checkpoint inhibitor in head and neck squamous cell carcinoma. Given the range of antibodies and platforms for PD-L1 testing, it is essential to understand the performance of different staining and scoring methods. PD-L1 expression in 156 head and neck mucosal squamous cell carcinoma (HNmSCC) cases at Asan Medical Center was assessed using 106 tissue microarray (TMA) cores and 50 whole slides. Three standardised PD-L1 assays (22C3 pharmDx, SP263, and 28-8 pharmDx) and one laboratory-developed test (22C3 LDT) were evaluated: the combined positive score (CPS) with ≥1, ≥20, and ≥50 cut-offs, and the tumour positive score (TPS) with ≥1%, ≥20%, ≥50% cut-offs. Concordance on a continuous scale among the assays was good to excellent for CPS [intraclass correlation coefficient (ICC) range 0.73–0.94] and TPS (ICC range 0.70–0.94) and in both TMA and whole slides cohorts. Stratification by variable cut-offs demonstrated moderate to good agreement among most assays, as analysed by Gwet's AC1. PD-L1 expression was significantly correlated with tumour location using the 22C3 pharmDx assay (CPS, <em>p</em>=0.014; TPS, <em>p</em>=0.033). Notable concordance was found among PD-L1 assays, suggesting their potential interchangeability in HNmSCC.</div></div>","PeriodicalId":19915,"journal":{"name":"Pathology","volume":"56 7","pages":"Pages 969-981"},"PeriodicalIF":3.6,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142205227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-18DOI: 10.1016/j.pathol.2024.06.007
Arthur J. Morris , Sarah E. Kidd , Catriona L. Halliday , Sharon C-A. Chen , Wendy McKinney , Katherine Ryan , Juliet Elvy
Past analysis of laboratory methods used for mycology specimens revealed significant variation in practices, many of which fell short of recommended procedures. In 2016 these findings led to a set of recommendations for laboratories to consider modification of their methods where appropriate, to analyse current laboratory methods used by participants in the Royal College of Pathologists of Australasia Quality Assurance Programs (RCPAQAP) Mycology module, and to compare these to the 2016 recommendations.
Seven test items, with 105–107 participants each, were analysed. Several laboratories (7–12%) did not handle specimens as recommended in an appropriate biological safety cabinet. Direct microscopy was not performed on tissue specimens 23–25% of the time. The most used staining method was potassium hydroxide with an optical brightener for fluorescent microscopy (49%) followed by Gram stain (33%). While 17–25% of laboratories used three or more media, use of four or more was uncommon (<3%). Between 9–13% of participants used only a single non-inhibitory medium for cultures. Urine specimens were incubated longer than recommended with 57% of laboratories incubating for >7days and 24% >21 days. Duration of incubation was shorter than recommended for several specimen types with 36% of skin specimens and 37–48% of tissue specimens being kept ≤21 days. For cultures kept >7 days, 13% were inspected daily, but for those incubating >14 days only 3%.
The methods of several laboratories remain outside recommended practice. An updated set of recommendations are made.
{"title":"Update on methods used for mycological testing: wide diversity and opportunities for improvement persist","authors":"Arthur J. Morris , Sarah E. Kidd , Catriona L. Halliday , Sharon C-A. Chen , Wendy McKinney , Katherine Ryan , Juliet Elvy","doi":"10.1016/j.pathol.2024.06.007","DOIUrl":"10.1016/j.pathol.2024.06.007","url":null,"abstract":"<div><div>Past analysis of laboratory methods used for mycology specimens revealed significant variation in practices, many of which fell short of recommended procedures. In 2016 these findings led to a set of recommendations for laboratories to consider modification of their methods where appropriate, to analyse current laboratory methods used by participants in the Royal College of Pathologists of Australasia Quality Assurance Programs (RCPAQAP) Mycology module, and to compare these to the 2016 recommendations.</div><div>Seven test items, with 105–107 participants each, were analysed. Several laboratories (7–12%) did not handle specimens as recommended in an appropriate biological safety cabinet. Direct microscopy was not performed on tissue specimens 23–25% of the time. The most used staining method was potassium hydroxide with an optical brightener for fluorescent microscopy (49%) followed by Gram stain (33%). While 17–25% of laboratories used three or more media, use of four or more was uncommon (<3%). Between 9–13% of participants used only a single non-inhibitory medium for cultures. Urine specimens were incubated longer than recommended with 57% of laboratories incubating for >7days and 24% >21 days. Duration of incubation was shorter than recommended for several specimen types with 36% of skin specimens and 37–48% of tissue specimens being kept ≤21 days. For cultures kept >7 days, 13% were inspected daily, but for those incubating >14 days only 3%.</div><div>The methods of several laboratories remain outside recommended practice. An updated set of recommendations are made.</div></div>","PeriodicalId":19915,"journal":{"name":"Pathology","volume":"56 7","pages":"Pages 1021-1027"},"PeriodicalIF":3.6,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-18DOI: 10.1016/j.pathol.2024.06.008
Mark James Wilsher , James D. Bedford
{"title":"Combination of lentiginous junctional melanocytic naevus, Toker cell hyperplasia and pagetoid dyskeratosis of the nipple: a potential diagnostic pitfall","authors":"Mark James Wilsher , James D. Bedford","doi":"10.1016/j.pathol.2024.06.008","DOIUrl":"10.1016/j.pathol.2024.06.008","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":"56 7","pages":"Pages 1053-1055"},"PeriodicalIF":3.6,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142205257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-14DOI: 10.1016/j.pathol.2024.06.004
Kyle M. Hatton-Jones , Nicholas P. West , Jean Barcelon , Amanda J. Cox
The emergence of spatial profiling technologies in recent years has accelerated opportunities to profile in detail the molecular attributes of a wide range of tissue pathologies using archival specimens. However, tissue treatment for fixation and storage does not always support generation of high-quality genomic data. The purpose of this study was to investigate the impacts of Proteinase K (ProtK) treatment, as a way to increase target transcript exposure, on downstream sequencing data quality metrics for spatial transcriptomic data using formalin-fixed, paraffin-embedded samples. In a series of four independent assessments using different tissue types (nasal mucosa, tonsil, pancreas), varying concentrations of ProtK (ranging from 0.1 to 1 μg/mL) were used as part of the sample processing workflow to generate transcriptomic data using the Nanostring GeoMx DSP and Illumina NextSeq 2000 platforms. Use of higher concentrations of ProtK was generally found to increase total reads (2–4-fold). However, negative probe counts also tended to be increased (2–12-fold), resulting in reductions in the signal-to-noise ratio (10–70% lower) and the number of genes detected above background (50–80% lower). These effects were not seen in all tissues and impacts of tissue handling and processing, beyond ProtK treatment, on data quality metrics, also require consideration. Regardless, these observations highlight the need for careful consideration of a range of sample processing factors and benefits that may be achieved through the optimisation of sample processing workflows for specific tissues as a way to maximise the generation of quality data using spatial transcriptomic approaches.
{"title":"The effect of Proteinase K treatment on GeoMx digital spatial profiling data quality from formalin-fixed, paraffin-embedded tissue","authors":"Kyle M. Hatton-Jones , Nicholas P. West , Jean Barcelon , Amanda J. Cox","doi":"10.1016/j.pathol.2024.06.004","DOIUrl":"10.1016/j.pathol.2024.06.004","url":null,"abstract":"<div><div>The emergence of spatial profiling technologies in recent years has accelerated opportunities to profile in detail the molecular attributes of a wide range of tissue pathologies using archival specimens. However, tissue treatment for fixation and storage does not always support generation of high-quality genomic data. The purpose of this study was to investigate the impacts of Proteinase K (ProtK) treatment, as a way to increase target transcript exposure, on downstream sequencing data quality metrics for spatial transcriptomic data using formalin-fixed, paraffin-embedded samples. In a series of four independent assessments using different tissue types (nasal mucosa, tonsil, pancreas), varying concentrations of ProtK (ranging from 0.1 to 1 μg/mL) were used as part of the sample processing workflow to generate transcriptomic data using the Nanostring GeoMx DSP and Illumina NextSeq 2000 platforms. Use of higher concentrations of ProtK was generally found to increase total reads (2–4-fold). However, negative probe counts also tended to be increased (2–12-fold), resulting in reductions in the signal-to-noise ratio (10–70% lower) and the number of genes detected above background (50–80% lower). These effects were not seen in all tissues and impacts of tissue handling and processing, beyond ProtK treatment, on data quality metrics, also require consideration. Regardless, these observations highlight the need for careful consideration of a range of sample processing factors and benefits that may be achieved through the optimisation of sample processing workflows for specific tissues as a way to maximise the generation of quality data using spatial transcriptomic approaches.</div></div>","PeriodicalId":19915,"journal":{"name":"Pathology","volume":"56 7","pages":"Pages 1028-1035"},"PeriodicalIF":3.6,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lupoid cutaneous leishmaniasis in Pakistan: a case series in school children","authors":"Asma Ashraf, Saima Qadeer, Ume Amara Bukhari, Umme Salma","doi":"10.1016/j.pathol.2024.06.005","DOIUrl":"10.1016/j.pathol.2024.06.005","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":"56 7","pages":"Pages 1049-1051"},"PeriodicalIF":3.6,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-13DOI: 10.1016/j.pathol.2024.05.014
Xuan Wang , Hongmei Yi , Qingxiao Liu , Tuanjie Guo , Anqi Li , Binshen Ouyang , Yimin Li , Yuxiu Zhang , Haimin Xu , Lei Dong , Xu Wang , Chaofu Wang
Anaplastic lymphoma kinase-positive large B-cell lymphoma (ALK+ LBCL) is a rare and highly aggressive lymphoma with characteristic ALK rearrangements. Various fusion genes involving ALK have been demonstrated, but the influence of the ALK fusion partners on ALK protein expression and the genetic characteristics of ALK+ LBCL remain relatively unknown. In this study, we conducted an extensive clinicopathological and molecular analysis on seven cases of ALK+ LBCL to explore the correlation between ALK fusion genes and ALK protein expression, thereby enriching the genetic characteristics of this tumour. We integrated the findings from clinical, histopathological/immunophenotypic, and molecular studies, including three samples subjected to next-generation sequencing, and six cases underwent RNA-based ALK fusion gene detection. We identified five distinct types of ALK fusion genes, including CLTC, NPM1, PABPC1, SEC31A, and TFG. Notably, only the NPM1::ALK fusion showed nuclear and cytoplasmic ALK staining, and the remaining four fusion genes resulted in cytoplasmic ALK staining. Our analysis revealed that the CLTC::ALK fusion resulted in a unique cytoplasmic perinuclear Golgi zone focal granular heterogeneous staining pattern of ALK. Additionally, we identified six potentially clinically significant gene mutations, including TET2, CHD2, DTX1, KMT2D, LRP1B, and XPO1. Furthermore, in all cases, the absence of 5-hydroxymethylcytosine (5hmC) was observed. We present seven cases of ALK+ LBCL, discussing the correlation between fusion genes and ALK protein expression, and enhancing our understanding of the genetic attributes of this tumour. This study also shows the loss of 5hmC in nearly all seven ALK+ LBCL cases, independently of TET2 mutations.
{"title":"ALK-positive large B-cell lymphoma: a clinicopathological and molecular characteristics analysis of seven cases","authors":"Xuan Wang , Hongmei Yi , Qingxiao Liu , Tuanjie Guo , Anqi Li , Binshen Ouyang , Yimin Li , Yuxiu Zhang , Haimin Xu , Lei Dong , Xu Wang , Chaofu Wang","doi":"10.1016/j.pathol.2024.05.014","DOIUrl":"10.1016/j.pathol.2024.05.014","url":null,"abstract":"<div><div>Anaplastic lymphoma kinase-positive large B-cell lymphoma (ALK<sup>+</sup> LBCL) is a rare and highly aggressive lymphoma with characteristic <em>ALK</em> rearrangements. Various fusion genes involving <em>ALK</em> have been demonstrated, but the influence of the <em>ALK</em> fusion partners on ALK protein expression and the genetic characteristics of ALK<sup>+</sup> LBCL remain relatively unknown. In this study, we conducted an extensive clinicopathological and molecular analysis on seven cases of ALK<sup>+</sup> LBCL to explore the correlation between <em>ALK</em> fusion genes and ALK protein expression, thereby enriching the genetic characteristics of this tumour. We integrated the findings from clinical, histopathological/immunophenotypic, and molecular studies, including three samples subjected to next-generation sequencing, and six cases underwent RNA-based <em>ALK</em> fusion gene detection. We identified five distinct types of <em>ALK</em> fusion genes, including <em>CLTC, NPM1, PABPC1, SEC31A</em>, and <em>TFG</em>. Notably, only the <em>NPM1::ALK</em> fusion showed nuclear and cytoplasmic ALK staining, and the remaining four fusion genes resulted in cytoplasmic ALK staining. Our analysis revealed that the <em>CLTC::ALK</em> fusion resulted in a unique cytoplasmic perinuclear Golgi zone focal granular heterogeneous staining pattern of ALK. Additionally, we identified six potentially clinically significant gene mutations, including <em>TET2, CHD2, DTX1, KMT2D, LRP1B</em>, and <em>XPO1</em>. Furthermore, in all cases, the absence of 5-hydroxymethylcytosine (5hmC) was observed. We present seven cases of ALK<sup>+</sup> LBCL, discussing the correlation between fusion genes and ALK protein expression, and enhancing our understanding of the genetic attributes of this tumour. This study also shows the loss of 5hmC in nearly all seven ALK<sup>+</sup> LBCL cases, independently of <em>TET2</em> mutations.</div></div>","PeriodicalId":19915,"journal":{"name":"Pathology","volume":"56 7","pages":"Pages 961-968"},"PeriodicalIF":3.6,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adult T-cell leukaemia/lymphoma (ATLL) is an aggressive peripheral T-cell neoplasm with a poor prognosis. T-cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domains (TIGIT) is an immune checkpoint receptor expressed on T and natural killer cells. Although increased TIGIT expression in the tumour microenvironment is associated with poor prognosis in various neoplasms, its relevance in ATLL remains unknown. Herein, we investigated the clinicopathological impact of TIGIT expression on ATLL using immunohistochemistry. TIGIT expression was detected in 21 of 84 patients (25%). A partial association between the clinical features and immune checkpoint molecules and the expression of TIGIT was found including sIL-2R, CD86 and GITR. TIGIT-positive patients [median survival time (MST) 8.9 months, 95% confidence interval (CI) 7.7–15.6] had inferior overall survival compared with TIGIT-negative patients (MST 18.7 months, 95% CI 12.0–36.4) (p=0.0124]. TIGIT expression maintained its prognostic value for overall survival in both univariate and multivariate analyses [hazard ratio (HR) 1.909; 95% CI 1.044–3.488; p=0.0356]. Further studies are required to clarify the clinical and biological significance of TIGIT expression in patients with ATLL.
成人 T 细胞白血病/淋巴瘤（ATLL）是一种侵袭性外周 T 细胞肿瘤，预后较差。具有免疫球蛋白和免疫受体酪氨酸抑制结构域的T细胞免疫受体（TIGIT）是一种免疫检查点受体，在T细胞和自然杀伤细胞上表达。虽然肿瘤微环境中 TIGIT 表达的增加与多种肿瘤的不良预后有关，但其与 ATLL 的相关性仍不清楚。在此，我们采用免疫组化方法研究了 TIGIT 表达对 ATLL 的临床病理影响。84 例患者中有 21 例（25%）检测到 TIGIT 表达。临床特征和免疫检查点分子与 TIGIT 表达之间存在部分关联，包括 sIL-2R、CD86 和 GITR。与TIGIT阴性患者（中位生存时间（MST）18.7个月，95% 置信区间（CI）12.0-36.4）相比，TIGIT阳性患者（中位生存时间（MST）8.9个月，95% 置信区间（CI）7.7-15.6）的总生存率较低（=0.0124）。在单变量和多变量分析中，TIGIT表达对总生存期仍有预后价值[危险比（HR）1.909；95% CI 1.044-3.488；=0.0356]。要明确TIGIT表达在ATLL患者中的临床和生物学意义，还需要进一步的研究。
{"title":"TIGIT expression on neoplastic cells is a poor prognostic factor for adult T-cell leukaemia/lymphoma","authors":"Yuichi Yamada , Hiroaki Miyoshi , Mai Takeuchi , Kazutaka Nakashima , Kyohei Yamada , Takeharu Kato , Ken Tanaka , Kei Kohno , Yoshitaka Imaizumi , Yasushi Miyazaki , Koichi Ohshima","doi":"10.1016/j.pathol.2024.06.003","DOIUrl":"10.1016/j.pathol.2024.06.003","url":null,"abstract":"<div><div>Adult T-cell leukaemia/lymphoma (ATLL) is an aggressive peripheral T-cell neoplasm with a poor prognosis. T-cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domains (TIGIT) is an immune checkpoint receptor expressed on T and natural killer cells. Although increased TIGIT expression in the tumour microenvironment is associated with poor prognosis in various neoplasms, its relevance in ATLL remains unknown. Herein, we investigated the clinicopathological impact of TIGIT expression on ATLL using immunohistochemistry. TIGIT expression was detected in 21 of 84 patients (25%). A partial association between the clinical features and immune checkpoint molecules and the expression of TIGIT was found including sIL-2R, CD86 and GITR. TIGIT-positive patients [median survival time (MST) 8.9 months, 95% confidence interval (CI) 7.7–15.6] had inferior overall survival compared with TIGIT-negative patients (MST 18.7 months, 95% CI 12.0–36.4) (<em>p</em>=0.0124]. TIGIT expression maintained its prognostic value for overall survival in both univariate and multivariate analyses [hazard ratio (HR) 1.909; 95% CI 1.044–3.488; <em>p</em>=0.0356]. Further studies are required to clarify the clinical and biological significance of TIGIT expression in patients with ATLL.</div></div>","PeriodicalId":19915,"journal":{"name":"Pathology","volume":"56 7","pages":"Pages 993-999"},"PeriodicalIF":3.6,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142205258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-12DOI: 10.1016/j.pathol.2024.05.013
Hemamali Samaratunga , Brett Delahunt , Mats Olsson , Markus Aly , Lars Egevad , John Yaxley
{"title":"Benign prostatic hyperplasia and insignificant prostate cancer with very high levels of serum prostate specific antigen","authors":"Hemamali Samaratunga , Brett Delahunt , Mats Olsson , Markus Aly , Lars Egevad , John Yaxley","doi":"10.1016/j.pathol.2024.05.013","DOIUrl":"10.1016/j.pathol.2024.05.013","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":"56 7","pages":"Pages 1059-1061"},"PeriodicalIF":3.6,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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