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Sulfate Reducing Bacterial Corrosion of Mild Steel in Liquid and Solid Media 硫酸盐降低低碳钢在液体和固体介质中的细菌腐蚀
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20220713160735
Sanaullah Sattar
This study was carried out to check the corrosive impact of sulfate-reducing bacteria (SRB) on mild steel coupons in solid and liquid media under two variable nutritional conditions. The bacterial species for the experimental trials was isolated from buried corroded metallic installment and found motile, Gram- negative, non-spore former and identified by 16S rRNA gene sequencing as Desulfovibrio desulfuricans . The corrosive impact of SRB on steel coupons was performed in water as liquid medium and the processed clay as the solid medium without and with the provision of Postgate B medium. After 60 days of anaerobic incubation, corrosion rate (CR) and average percent weight loss (APWL) of the steel pieces were calculated. Higher CR and APWL were observed in the liquid medium (water) than in the solid medium (clay). The CR and APWL in water were 249 ± 2 µg dm −2 d −1 and 4.16 ± 0.25 %, respectively, while the corresponding figures for CR and APWL in clay were 108 ± 2 and 1.88 ± 0.29, respectively. In addition, the CR and APWL were higher in the presence of Postgate B medium compared to the medium without it. X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopic analyses reveals the presence of FeS in the corrosion product. Our findings of this study will be helpful for devising corrosion-protecting strategies.
研究了硫酸盐还原菌(SRB)在固体和液体两种不同营养条件下对低碳钢片的腐蚀影响。从埋地腐蚀金属装置中分离到活性的革兰氏阴性非孢子原体,经16S rRNA基因测序鉴定为Desulfovibrio脱硫菌。在不添加和添加Postgate B介质的情况下,以水为液体介质,以加工过的粘土为固体介质进行了SRB对钢片的腐蚀作用。经过60 d的厌氧培养,计算钢件的腐蚀速率(CR)和平均失重率(APWL)。液体介质(水)的CR和APWL高于固体介质(粘土)。水中CR和APWL分别为249±2µg dm−2 d−1和4.16±0.25%,粘土中CR和APWL分别为108±2和1.88±0.29。此外,与未添加Postgate B培养基相比,添加Postgate B培养基的CR和APWL更高。x射线衍射(XRD)和傅里叶变换红外(FTIR)光谱分析表明,腐蚀产物中存在FeS。我们的研究结果将有助于制定腐蚀防护策略。
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引用次数: 1
Effect of Whole Allium cepa Linn. Bulb Slurry on Haematological and Biochemical Components of Clarias gariepinus Juveniles 全葱的药理作用。球茎浆液对小Clarias gariepinus幼鱼血液学和生化成分的影响
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20200828170831
O. M. Oghenochuko
1Animal Science Department, Landmark University, PMB1001, Omu-Aran, Kwara State, Nigeria. 2Department of Aquaculture and Fisheries Management, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun State, Nigeria. 3College of Veterinary Medicine, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun State, Nigeria. 4Landmark University SDG 2, Landmark University Omu-Aran, Kwara State, Nigeria Article Information Received 28 August 2020 Revised 10 February 2022 Accepted 29 March 2022 Available online 30 January 2023 (early access)
1 Landmark大学动物科学系,尼日利亚夸拉州Omu-Aran PMB1001 . 2联邦农业大学水产养殖与渔业管理系,尼日利亚奥贡州abobuta, PMB 2240 . 3联邦农业大学兽医学院,尼日利亚奥贡州abobuta, PMB 2240 . 4Landmark大学可持续发展目标2,尼日利亚夸拉州Omu-Aran Landmark大学,文章信息收到2020年8月28日修订2022年2月10日接受2022年3月29日2023年1月30日在线发布(早期获取)
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引用次数: 0
Food Preference of Rhesus Monkey (Macaca mulatta) in the Margalla Hills National Park, Islamabad, Pakistan 巴基斯坦伊斯兰堡玛加拉山国家公园的恒河猴(Macaca mulatta)食物偏好
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20210724200757
S. Aslam
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引用次数: 0
Optimization of Size Exclusion Chromatography for the Purification and Quantification of Foot and Mouth Disease Virus Serotype “O” 口蹄疫病毒“O”型分离纯化及定量的粒径排除色谱优化
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20211109021143
A. Razak
Foot and mouth disease (FMD) is economically devastating disease of livestock overwhelming the international trading of animal based products. The unpurified inactivated vaccine containing Foot and Mouth Disease Virus a (FMDV) and non-structural proteins (NSPs) used to control the disease stimulated the production of anti-NSP antibodies. Countries striving to establish FMD free status must ensure the absence of circulating FMDV and anti-NSP antibodies in vaccinated animals, which are produced in natural infection. Production of purified FMD vaccines contrary to other animal’s vaccines required extra step of virus purification from its NSPs. Present project was designed to optimize different parameters of size exclusion chromatography (SEC) for the purification of local isolate of FMDV from NSPs. For this purpose Bio-rad Econo-Column 15/50 was packed with Sephacryl TM S-300 resin and fitted with BioRad BioLogic LP Chromatographic system. Mixture of blue dextran and bovine serum albumin used as biomarkers for FMDV and NSPs respectively was employed to optimize resin bed height in column based on resolution between curves, while that FMDV was used to optimize flow rate of mobile phase and sample volume based on column efficiency. The chromatographic fraction of FMDV was ensured for purity through protein analysis by SDS-PAGE and serologically by 3ABC-NSP ELISA. It was found that maximum resolution of 1.86 was achieved at 48cm of resin bed height followed by 1.5 and 1.27 at 40cm and 32cm respectively. FMDV was eluted earlier with retention time 138.02+2.02min at flow of 0.7ml/min than 165.76+1.28min at 0.5ml/min along with better column efficiency with sample volume at 4% of column volume. SEC could detect and quantify from 603.75µg/ml in PEG concentrated FMDV up to 3.33µg/ml in 1:64 dilution as lower limit of detection and quantification. Presence of two bands of structural protein with absence of NSPs bands on SDS-PAGE and negative NSP% in hyperimmune sera raised in goats revealed the successful elimination of NSPs contents from FMDV suspension by SEC.
口蹄疫是一种经济上具有破坏性的牲畜疾病,严重影响着畜产品的国际贸易。含有口蹄疫病毒a (FMDV)和用于控制口蹄疫的非结构蛋白(NSPs)的未纯化灭活疫苗刺激了抗nsp抗体的产生。努力建立无口蹄疫地位的国家必须确保在接种疫苗的动物中不存在流行的口蹄疫病毒和抗nsp抗体,这些抗体是在自然感染中产生的。生产纯化的口蹄疫疫苗与其他动物疫苗不同,需要从其NSPs中提纯病毒。本项目旨在优化不同粒径隔离层析(SEC)的参数,用于从NSPs中分离分离FMDV。为此,Bio-rad经济柱15/50用Sephacryl TM S-300树脂填充,并配备BioRad BioLogic LP色谱系统。蓝色葡聚糖和牛血清白蛋白混合物分别作为FMDV和NSPs的生物标志物,以曲线间分辨率为基础优化树脂层高度;以FMDV为基础,以柱效率为基础优化流动相流速和样品体积。通过SDS-PAGE蛋白分析和3ABC-NSP ELISA血清学分析确保FMDV的纯度。在树脂床高度48cm处分辨率最高,为1.86,其次是40cm和32cm处,分别为1.5和1.27。在0.7ml/min流速下,FMDV洗脱时间为138.02+2.02min,比在0.5ml/min流速下洗脱时间为165.76+1.28min更早,柱效率为4%。在PEG浓度的FMDV中,SEC可检测定量603.75µg/ml至1:64稀释的3.33µg/ml为检测定量下限。在SDS-PAGE上发现了两条不含NSPs的结构蛋白带,在山羊高免疫血清中发现了NSP%为阴性的结构蛋白带,这表明SEC成功地消除了FMDV悬液中NSPs的含量。
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引用次数: 0
Effect of the Combined Use of Beauveria bassiana (Balsamo) Vuillemin and Diatomaceous Earth for the Control of Tribolium castaneum (Herbst) and Trogoderma granarium Everts 球孢白僵菌与硅藻土联合施用防治木参、木参线虫的效果研究
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20211120151100
Muhammad Yasin
Muhammad Yasin1, Azhar Abbas Khan2*, Sana Rubab2 and Sumaira Maqsood3 1Deprtment of Entomology, The Islamia University, Bahawalpur, Pakistan. 2College of Agriculture, Bahauddin Zakariya University, Bahadur Sub Campus Layyah, Pakistan. 3Department of Environmental Sciences, Faculty of Mountain Agriculture and Environmental Sciences, Kohsar University, Murree, Pakistan. Article Information Received 20 November 2021 Revised 11 August 2022 Accepted 08 September 2022 Available online 05 January 2023 (early access)
Muhammad Yasin1, Azhar Abbas Khan2*, Sana Rubab2和Sumaira maqsoo3 1巴基斯坦巴哈瓦尔布尔伊斯兰大学昆虫学系2巴基斯坦巴哈瓦尔布尔扎卡里亚大学农学院,巴哈杜尔分校Layyah 3巴基斯坦Kohsar大学山地农业与环境科学学院环境科学系文章信息收到2021年11月20日修订2022年8月11日接受2022年9月08日在线发布2023年1月05日(早期访问)
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引用次数: 0
Blue Light Color Reduces the Newcastle Disease Post-Vaccinal Reactions of Indian River Broilers under Egyptian Conditions 蓝光降低埃及条件下印度河肉鸡新城疫疫苗后反应
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20220323080346
T. M. Mousa-Balabel
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引用次数: 0
Effect of Urea Treated Sugarcane Bagasse on Growth, Proximate Composition, Microbial Flora and Digestive Enzymes Activities of Grass Carp (Ctenopharyngodon idella) 尿素处理甘蔗渣对草鱼生长、近似组成、微生物区系及消化酶活性的影响
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20220524090521
Ayub Khawar
A 90 days experimental trial was conducted to evaluate the effect of urea treated sugarcane bagasse on the growth, gut microflora and digestive enzymes of grass carp ( Ctenopharyngodon idella ). Fish were fed to 3% wet body weight per day with experimental diets having 0% (CTRL), 0.7% (T1), 1.4% (T2) and 2.1% (T3) urea, with each group having two replicate tanks. Fish fed on 2.1% urea treated sugarcane bagasse (T3) showed significantly (P<0.05) higher growth as compared to T2, T1 and CTRL. The feed conversion ratio and specific growth rate were also significantly higher in T3 fish group, followed by the T2, T1 and CTRL groups. Proximate analysis of fish showed that the level of crude protein was higher significantly in T2 fish than other groups and crude fat level was significantly higher in T1 fish, followed the T2, CTRL and T3 fish groups. The percentage level of dry matter was significantly higher in T3 fish and ash percentage was significantly higher in T2 fish relative to T3, T1 and CTRL groups. Amylase and Lipase concentration was significantly higher in T2 fish compared with T3, T1 and CTRL groups. Protease concentration was significantly higher in T3 fish, followed by the T1, T2 and CTRL fish groups. Lastly, the presence of Lactobacillus fermentum was confirmed in the T1, T2 and T3 fish groups. Overall, the results showed that urea treated sugarcane bagasse can be used as a feed ingredient for C. idella and has no adverse effect on the nutritional value of fish.
本试验旨在研究尿素处理甘蔗渣对草鱼生长、肠道菌群和消化酶的影响。试验日粮中尿素含量分别为0% (CTRL)、0.7% (T1)、1.4% (T2)和2.1% (T3),饲喂湿重为3%的试验饲料,每组设2个重复槽。饲喂2.1%尿素处理的甘蔗渣(T3)的鱼的生长显著高于(P<0.05) T2、T1和CTRL。T3组的饲料系数和特定生长率也显著高于T2、T1和CTRL组。鱼类近似分析表明,T2组鱼的粗蛋白质水平显著高于其他各组,T1组鱼的粗脂肪水平显著高于其他各组,其次是T2、CTRL和T3组。与T3、T1和CTRL组相比,T3组干物质含量显著高于T2组,T2组灰分含量显著高于对照组。T2组的淀粉酶和脂肪酶浓度显著高于T3、T1和CTRL组。T3组的蛋白酶浓度显著高于对照组,其次是T1、T2和CTRL组。最后,在T1、T2和T3鱼组中证实了发酵乳杆菌的存在。综上所述,经尿素处理的甘蔗渣可作为idella的饲料原料,对鱼类的营养价值无不良影响。
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引用次数: 0
Role of miRNAs and their Target Genes in Diabetic Foot Ulcer Wound Healing mirna及其靶基因在糖尿病足溃疡创面愈合中的作用
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20220528200554
Muhammad Shakil Khan
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引用次数: 0
Effect of Duckweed-Based Aquafeeds on Feed Digestibility and Growth Performance of Gibelion catla 以浮萍为基础的水产饲料对吉贝隆鲇鱼饲料消化率和生长性能的影响
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20220722090715
J. Shafi
The present study was conducted to find viable and economic ingredients for fish feed by evaluating the effect of partial fish meal replacement with duckweed ( Lemna minor ) in Gibelion catla feed. Four different isonitrogenous feeds (with 0, 10, 20 and 30% replacement of fish meal with) were prepared and fed to juvenile catla for 60 days at the rate of 5% fish biomass. No significant difference ( P >0.05) in fish growth parameters and feed efficiency was observed among the treatments. There was no significant difference in apparent digestibility coefficient (ADC) of dry matter among treatments. Feed ADC (protein) with duckweed at 30% inclusion level was significantly lower ( P <0.05) compared to the other treatments. There was no significant difference in muscle composition of fish reared under the four treatments. Results of the present study showed that duckweed can be used to replace up to 20% of fish meal in feed of catla without any negative effect on fish growth or feed digestibility.
本研究旨在通过对大黄颡鱼饲料中浮萍部分替代鱼粉的效果进行评价,寻找可行且经济的鱼饲料成分。配制4种不同的等氮饲料(鱼粉替代量分别为0%、10%、20%和30%),以5%的鱼生物量投喂幼鱼60 d。各处理对鱼的生长参数和饲料效率无显著影响(P < 0.05)。干物质表观消化系数(ADC)在各处理间无显著差异。30%浮萍水平的饲料ADC(蛋白质)显著低于其他处理(P <0.05)。4种处理对鱼的肌肉组成无显著影响。本研究结果表明,浮萍可替代鲶鱼饲料中高达20%的鱼粉,对鱼的生长和饲料消化率没有负面影响。
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引用次数: 0
A Review of 20 Years Research Progression on Prodrug Technology in Inhaled Medications 吸入药物前药技术20年研究进展综述
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20220813160811
Lijuan Liu
.
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引用次数: 0
期刊
Pakistan Journal of Zoology
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