Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20230315000312
Ming-Hao Yu
Tannic acid, as a polyphenol, is widely present in dicotyledonous plants and angiosperms. The study found that tannins could affect the ovarian development of Brandt’s voles. However, the specific effects of tannic acid on reproduction and its underlying mechanisms are still poorly understood. Therefore, this study explored the effects of tannic acid on the reproduction of Brandt’s voles. We treated female Brandt’s voles from four weeks postpartum with varying tannic acid doses (0 (control), 0.3% (low dose) or 0.6% (high dose) tannic acids) until 8 weeks (puberty) and 13 weeks (sexual maturity)). Ovarian specimens were collected for immunohistochemical analysis and RT-PCR. The treated Brandt’s voles were mated with male Brandt’s voles of the same age and we recorded the number of embryos, litters and the male to female ratio of the offspring. We found that the female Brandt’s voles in the low-dose tannic acid group had enhanced reproductive abilities, characterized by reduced follicular atresia and increased numbers of embryos and litters of offspring. The different doses of tannic acid altered the female-male ratio of the Brandt’s voles’ offspring. While the low-dose of tannic acid enhanced the level of ovarian autophagy by significantly increasing the expression of Beclin1 and LC3 proteins in the ovaries of the Brandt’s voles during sexual maturity. In short, tannic acid can promote the reproduction of Brandt’s voles by affecting the level of ovarian autophagy and improving the development of follicles. Tannins can boost the reproduction of Brandt’s voles.
{"title":"Tannic Acid Regulates Autophagy in the Ovary of Female Brandt’s Voles Affecting their Reproduction","authors":"Ming-Hao Yu","doi":"10.17582/journal.pjz/20230315000312","DOIUrl":"https://doi.org/10.17582/journal.pjz/20230315000312","url":null,"abstract":"Tannic acid, as a polyphenol, is widely present in dicotyledonous plants and angiosperms. The study found that tannins could affect the ovarian development of Brandt’s voles. However, the specific effects of tannic acid on reproduction and its underlying mechanisms are still poorly understood. Therefore, this study explored the effects of tannic acid on the reproduction of Brandt’s voles. We treated female Brandt’s voles from four weeks postpartum with varying tannic acid doses (0 (control), 0.3% (low dose) or 0.6% (high dose) tannic acids) until 8 weeks (puberty) and 13 weeks (sexual maturity)). Ovarian specimens were collected for immunohistochemical analysis and RT-PCR. The treated Brandt’s voles were mated with male Brandt’s voles of the same age and we recorded the number of embryos, litters and the male to female ratio of the offspring. We found that the female Brandt’s voles in the low-dose tannic acid group had enhanced reproductive abilities, characterized by reduced follicular atresia and increased numbers of embryos and litters of offspring. The different doses of tannic acid altered the female-male ratio of the Brandt’s voles’ offspring. While the low-dose of tannic acid enhanced the level of ovarian autophagy by significantly increasing the expression of Beclin1 and LC3 proteins in the ovaries of the Brandt’s voles during sexual maturity. In short, tannic acid can promote the reproduction of Brandt’s voles by affecting the level of ovarian autophagy and improving the development of follicles. Tannins can boost the reproduction of Brandt’s voles.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67575242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20230423120401
A. Hussain
Lentinula edodes C91-3 is an edible mushroom with demonstrated medicinal activity against various types of cancer in vitro and in vivo . The gene for Latcripin-5 (LP-5) was obtained from Lentinula edodes strain C 91-3 and inserted into a pET32a (+) vector, which was then, expressed in the Rosetta gami (DE3) strain. The purified LP-5 protein was analyzed using SDS-PAGE and western blot. The optimal solubilization parameters were found to be 0.6 mM IPGT for 6 h incubation at 37 ℃ . The solubilized protein was refolded using a refolding buffer and then dialyzed and concentrated using a dialysis buffer and PEG 20000, respectively. Phyre2 bioinformatics tool was used for protein modelling. The concentrated LP-5 protein was tested for its effect on the viability and cytotoxicity of various cancerous and non-cancerous cell lines using a cell counting kit-8 (CCK-8) assay. The LP-5 protein had the lowest IC50 value against the liver cancer line HepG2, at 58.15 μg/ml. Dose-and time-dependent morphological changes, such as cell shrinkage, blebbing formation, and cell fragmentation, were observed in treated cells. Apoptosis markers were evaluated using qPCR, flow cytometry, and western blot, and it was found that LP-5 protein increased the expression of Bax, Caspass-3, -8, -9, Cytochrome-C, and PARP, while decreasing the expression of Bcl2. Cell cycle arrest was also analyzed through qPCR, flow cytometry. Western blot results showed upregulated p21 and p27while CDK2, CDK4, CDK6, Cycline D1, and Cycline E1 were downregulated. These results suggest that LP-5 protein has potential as an anticancer agent against liver cancer cells.
{"title":"Expression, Characterization and Purification of Latcripin-5 from Lentinula edodes Strain C91-3 and its In Vitro Anticancer Activities","authors":"A. Hussain","doi":"10.17582/journal.pjz/20230423120401","DOIUrl":"https://doi.org/10.17582/journal.pjz/20230423120401","url":null,"abstract":"Lentinula edodes C91-3 is an edible mushroom with demonstrated medicinal activity against various types of cancer in vitro and in vivo . The gene for Latcripin-5 (LP-5) was obtained from Lentinula edodes strain C 91-3 and inserted into a pET32a (+) vector, which was then, expressed in the Rosetta gami (DE3) strain. The purified LP-5 protein was analyzed using SDS-PAGE and western blot. The optimal solubilization parameters were found to be 0.6 mM IPGT for 6 h incubation at 37 ℃ . The solubilized protein was refolded using a refolding buffer and then dialyzed and concentrated using a dialysis buffer and PEG 20000, respectively. Phyre2 bioinformatics tool was used for protein modelling. The concentrated LP-5 protein was tested for its effect on the viability and cytotoxicity of various cancerous and non-cancerous cell lines using a cell counting kit-8 (CCK-8) assay. The LP-5 protein had the lowest IC50 value against the liver cancer line HepG2, at 58.15 μg/ml. Dose-and time-dependent morphological changes, such as cell shrinkage, blebbing formation, and cell fragmentation, were observed in treated cells. Apoptosis markers were evaluated using qPCR, flow cytometry, and western blot, and it was found that LP-5 protein increased the expression of Bax, Caspass-3, -8, -9, Cytochrome-C, and PARP, while decreasing the expression of Bcl2. Cell cycle arrest was also analyzed through qPCR, flow cytometry. Western blot results showed upregulated p21 and p27while CDK2, CDK4, CDK6, Cycline D1, and Cycline E1 were downregulated. These results suggest that LP-5 protein has potential as an anticancer agent against liver cancer cells.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67576240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20230228150209
Ming Wang
The objective of this study was to investigate the effect of tamsulosin combined with ginger moxibustion on lower urinary tract symptoms in elderly patients with prostatic hyperplasia. It provides basis and new way for the research and treatment of BPH. A total of 80 elderly patients with BPH and lower urinary tract symptoms admitted from March 2021 to July 2022 in our hospital were selected for the study. The patients were separated into ginger moxibustion group (G) with 40 patients and combined group (J) with 40 patients. Among them, the patients in Group G were only treated with ginger, while oral tamsulosin sustained release capsule combined with ginger was used in Group J. The course of treatment of both groups is 1 month, and the treatment is in progress. The changes of IPSS, QOL, residual urine volume, bladder volume, TCM symptom score and SF-36 score of patients in Group G and Group J were compared. Compared with Group G, the total IPSS score, SSS score, QOL score, bladder residual urine volume and TCM symptom score of Group J were lower ( P <0.05) after treatment. The MFR, SF-36 score and the treatment efficiency in Group J were higher ( P <0.05). Compared with simple ginger moxibustion, tamsulosin combined with ginger moxibustion can effectively improve the lower urinary tract symptoms of elderly patients with benign prostatic hyperplasia.
{"title":"Effect of Tamsulosin on Lower Urinary Tract Symptoms in Elderly Patients with Benign Prostatic Hyperplasia","authors":"Ming Wang","doi":"10.17582/journal.pjz/20230228150209","DOIUrl":"https://doi.org/10.17582/journal.pjz/20230228150209","url":null,"abstract":"The objective of this study was to investigate the effect of tamsulosin combined with ginger moxibustion on lower urinary tract symptoms in elderly patients with prostatic hyperplasia. It provides basis and new way for the research and treatment of BPH. A total of 80 elderly patients with BPH and lower urinary tract symptoms admitted from March 2021 to July 2022 in our hospital were selected for the study. The patients were separated into ginger moxibustion group (G) with 40 patients and combined group (J) with 40 patients. Among them, the patients in Group G were only treated with ginger, while oral tamsulosin sustained release capsule combined with ginger was used in Group J. The course of treatment of both groups is 1 month, and the treatment is in progress. The changes of IPSS, QOL, residual urine volume, bladder volume, TCM symptom score and SF-36 score of patients in Group G and Group J were compared. Compared with Group G, the total IPSS score, SSS score, QOL score, bladder residual urine volume and TCM symptom score of Group J were lower ( P <0.05) after treatment. The MFR, SF-36 score and the treatment efficiency in Group J were higher ( P <0.05). Compared with simple ginger moxibustion, tamsulosin combined with ginger moxibustion can effectively improve the lower urinary tract symptoms of elderly patients with benign prostatic hyperplasia.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67574442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20220712170720
Sana Eijaz
{"title":"A New Herbal Combination and Trigonella foenum graceum Improve Insulin Resistance, Insulin Signaling Genes, Adipokines Level and Body Weight in Type 2 Diabetic Rat Model","authors":"Sana Eijaz","doi":"10.17582/journal.pjz/20220712170720","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220712170720","url":null,"abstract":"","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67562996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20190729100714
Hui-juan Yan
Hui-juan Yan1, Wei Wang2,*, Jian-qiu Yu1, Jun Yi2, Li-li Niu1, Hong-wei Chen1, Yu Qu1, Yang Pu 1, Ang Chen1, Yan Zhong1, Wei-gang Chen1 and Xing-ming Yu1 1Chengdu Zoo, Chengdu, Sichuan, P.R. China 2Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, Sichuan, P.R. China Article Information Received 29 July 2019 Revised 12 May 2020 Accepted 20 January 2021 Available online 21 July 2023 (early access)
{"title":"Age-Related Changes in the Gut Microbiota Composition of Hog Deer (Axis porcinus)","authors":"Hui-juan Yan","doi":"10.17582/journal.pjz/20190729100714","DOIUrl":"https://doi.org/10.17582/journal.pjz/20190729100714","url":null,"abstract":"Hui-juan Yan1, Wei Wang2,*, Jian-qiu Yu1, Jun Yi2, Li-li Niu1, Hong-wei Chen1, Yu Qu1, Yang Pu 1, Ang Chen1, Yan Zhong1, Wei-gang Chen1 and Xing-ming Yu1 1Chengdu Zoo, Chengdu, Sichuan, P.R. China 2Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, Sichuan, P.R. China Article Information Received 29 July 2019 Revised 12 May 2020 Accepted 20 January 2021 Available online 21 July 2023 (early access)","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67502340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20230207120254
Abeer Alahmari
{"title":"Aqueous Myrrh Extract Relieves Oxidative Stress-Dependent Nephrotoxicity Induced by Ethanol in Male Rats","authors":"Abeer Alahmari","doi":"10.17582/journal.pjz/20230207120254","DOIUrl":"https://doi.org/10.17582/journal.pjz/20230207120254","url":null,"abstract":"","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135157515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20230410070427
Rumisha Raza
{"title":"Molecular Identification of Predatory Birds Through Analysis of Mitochondrial ND2 Gene","authors":"Rumisha Raza","doi":"10.17582/journal.pjz/20230410070427","DOIUrl":"https://doi.org/10.17582/journal.pjz/20230410070427","url":null,"abstract":"","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135311741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20181110161130
Nageen Iqbal
The present study was conducted to check the growth performance and body composition of Labeo rohita fed with two different levels of fish meal replacement with bone meal. The trial was carried out in glass aquaria for a period of 8 weeks. Ten (n=10) fingerlings of L. rohita were reared per aquaria having capacity of 80-L water. Feed having 100% fish meal was considered as T1 and diets having 30% and 60% replacement of fish meal by bone meal as main protein source was considered as T2 and T3, respectively. Results showed that replacement of 30% and 60% fish meal with bone meal has better effect on growth of L. rohita analysed whole body composition revealed that lower values of total fat there was observed no significant differences in carbohydrates and moisture content of fish was observed. Bone meal could replace fish meal as an acceptable protein source to promote growth of L. rohita without any negative effects on growth and body composition.
{"title":"Analyses of Growth Performance and Whole Body Composition of Labeo rohita Fed with Bone Meal Diet","authors":"Nageen Iqbal","doi":"10.17582/journal.pjz/20181110161130","DOIUrl":"https://doi.org/10.17582/journal.pjz/20181110161130","url":null,"abstract":"The present study was conducted to check the growth performance and body composition of Labeo rohita fed with two different levels of fish meal replacement with bone meal. The trial was carried out in glass aquaria for a period of 8 weeks. Ten (n=10) fingerlings of L. rohita were reared per aquaria having capacity of 80-L water. Feed having 100% fish meal was considered as T1 and diets having 30% and 60% replacement of fish meal by bone meal as main protein source was considered as T2 and T3, respectively. Results showed that replacement of 30% and 60% fish meal with bone meal has better effect on growth of L. rohita analysed whole body composition revealed that lower values of total fat there was observed no significant differences in carbohydrates and moisture content of fish was observed. Bone meal could replace fish meal as an acceptable protein source to promote growth of L. rohita without any negative effects on growth and body composition.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67474939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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