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Insight into the Phylogenetic Position of Capreolus capreolus, the European Roe Deer, (Mammalia: Cervidae) from Turkey using Mitochondrial Genes 利用线粒体基因研究土耳其欧洲狍(哺乳动物:鹿科)的系统发育位置
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20220621070609
Perinçek Seçkinozan Şeker
For contributing to a better understanding of the phylogenetic position and evolutionary history of Turkish roe deer, mitochondrial DNA sequence data belonging to a population from Artvin, Turkey were introduced by this study. The cytochrome b (cyt b ) and cytochrome c oxidase subunit I (COI) genes of mitochondrial DNA belonging to the four samples of Capreolus capreolus were partially sequenced and analyzed alongside the sequences obtained from GenBank. The cyt b and COI data sets included sequences that were 1059 and 646 bp long, respectively. The Turkish roe deer population in Artvin had a relatively low nucleotide diversity and high haplotype diversity. A total of two haplotypes were identified for cyt b , while three haplotypes were identified for COI. Of the haplotypes detected, two cyt b and two COI haplotypes were specific to Turkey. Molecular phylogenetic analyses; Maximum Likelihood (ML), Bayesian Inference (BI), and Network revealed topologies and evolutionary networks which were not definitely resolved. Consequently, Turkish roe deer haplotypes were intertwined with the roe deer haplotypes from Central Europe instead of forming an independent evolutionary clade, and were also, found genetically close to Central European haplotypes. According to the evolutionary dating analysis, the divergence of C. capreolus populations in Europe started at the beginning of the Pleistocene (approximately 2.72 mya). This analysis also demonstrated that the divergence of Turkish roe deer began at approximately 0.58 mya. Given the existence of, shared haplotypes, the relative positions of Turkish roe deer haplotypes in the topologies of the ML and BI phylogenetic trees and evolutionary networks, and the times of evolutionary divergence events within this species; the following results may be inferred: (1) roe deer individuals with the same genotype as those in Europe can also be found in areas further east, such as Turkey; (2) these could be considered to be representatives, which may have contributed to the formation and shaping processes of the known admixed genetic structure of European roe deer populations in Europe; and finally, (3) the roe deer populations may have probably entered Turkey from Europe when evolutionary divergence times based on BI analysis are considered.
为了更好地了解土耳其狍子的系统发育位置和进化历史,本研究引入了土耳其阿尔特文一个种群的线粒体DNA序列数据。对4个样本的线粒体DNA细胞色素b (cyt b)和细胞色素c氧化酶亚基I (COI)基因进行了部分测序,并与GenBank中获得的序列进行了分析。cyt b和COI数据集的序列长度分别为1059和646 bp。阿特文地区土耳其狍子种群的核苷酸多样性较低,单倍型多样性较高。共鉴定出2个cyt b单倍型,3个COI单倍型。在检测到的单倍型中,两个cyt b和两个COI单倍型是土耳其特有的。分子系统发育分析;最大似然(ML)、贝叶斯推理(BI)和网络揭示了尚未确定解决的拓扑和进化网络。因此,土耳其狍的单倍型与来自中欧的狍的单倍型交织在一起,而不是形成一个独立的进化分支,而且在基因上也与中欧的单倍型接近。根据进化测年分析,欧洲C. capreolus种群的分化始于更新世初期(约2.72万年)。这一分析还表明,土耳其狍子的分化开始于大约0.58万年。考虑到共有单倍型的存在,土耳其狍子单倍型在ML和BI系统发育树和进化网络拓扑结构中的相对位置,以及该物种内进化分化事件的时间;可以推断出以下结果:(1)与欧洲相同基因型的狍个体也可以在更东的地区发现,如土耳其;(2)在欧洲已知的欧洲狍子种群混合遗传结构的形成和定型过程中,这些遗传结构可能具有一定的代表性;最后,(3)考虑到基于BI分析的进化分化时间,狍种群可能是从欧洲进入土耳其的。
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引用次数: 0
Identification of Nine Gastropod Species with Three New Records using Cytochrome Oxidase Sub-Unit 1 Marker 利用细胞色素氧化酶亚基1标记鉴定3个新记录的9种腹足动物
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20220701070727
Qurat-ul-Ain Ijaz
Qurat-ul-Ain Ijaz1, Amna Sulaman1, Dost Muhammad Baloch2, Muhammad Shafi2, Faiz Muhammad1* and Shahnaz Rashid1 1Center of Excellence in Marine Biology, University of Karachi, Karachi-75270, Pakistan 2Lasbella University of Agriculture, Water and Marine Sciences, Lasbella, Uthal Article Information Received 01 July 2022 Revised 15 August 2022 Accepted 14 September 2022 Available online 27 April 2023 (early access)
Qurat-ul-Ain Ijaz1, Amna Sulaman1, Dost Muhammad Baloch2, Muhammad Shafi2, Faiz Muhammad1*和Shahnaz Rashid1 1巴基斯坦卡拉奇-75270卡拉奇大学海洋生物学卓越中心2拉斯贝拉农业,水和海洋科学大学,拉斯贝拉,乌萨塔文章信息接收日期2022年7月1日修订2022年8月15日接受2022年9月14日2023年4月27日在线(提前获取)
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引用次数: 0
Effect of Nutritional Content on Artificially Supplied Seed Utilization of Wintering Birds in a Mixed Forest, South Korea 营养成分对混交林越冬鸟类人工补种利用的影响
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20220928070901
Hyun-Su Hwang
1Team of Specific Protected Area Research, National Institute of Ecology, Seocheon 33657, South Korea 2School of Bioresource and Bioscience, Chung-Ang University, Ansung, South Korea 3Animal Resources Division, National Institute of Biological Resources, Incheon 22689, South Korea. Article Information Received 28 September 2022 Revised 19 October 2022 Accepted 09 November 2022 Available online10 January 2023 (early access)
1国立生态研究所特定保护区研究小组,西川33657;2中央大学生物资源与生命科学学院,安城;3国立生物资源研究所动物资源处,仁川22689文章信息收到2022年9月28日修订2022年10月19日接受2022年11月9日在线发布2023年1月10日(早期访问)
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引用次数: 0
The Value of Procalcitonin Combined with C-Reactive Protein Assay in the Early Clinical Diagnosis of Bloodstream Infection 降钙素原联合c反应蛋白检测在血流感染早期临床诊断中的价值
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20221011021005
Cheng-Hong Chen
.
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引用次数: 0
Haemato-biochemical Changes and Various Risk Factors Associated with Bovine Coronavirus Infection in Cattle Calves 与牛冠状病毒感染相关的小牛血液生化变化和各种危险因素
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20220930120931
S. Abbas
Bovine coronavirus (BCoV) is a major viral pathogen linked to respiratory and enteric problems in newborn calves. The goal of this study is to look into the molecular occurrence, haemato-biochemical changes, and risk factors associated with the occurrence of BCoV infection in cattle calves at different dairy farms and small households in the district of Jhelum, Pakistan. From July 2020 to June 2021, 200 faecal samples were collected from newborn cattle calves exhibiting symptoms of diarrhoea and dysentery. S&C Biotech Bovine Coronavirus Antigen Rapid Test Kits were used to screen samples, which were then subjected to RT-PCR for molecular characterization. For the haemato-biochemical analysis, blood was taken from calves infected with BCoV. A questionnaire was used to collect information about the risk factors associated with the occurrence of BCoV infection. BCoV infection was found in 3.5% (7/200) of calves using diagnostic screening kits and 3.0% (6/200) of calves using RT-PCR. Breed, age, sex, vomiting, previous history of diarrhoea, body conditions, food type, colostrum feeding, deworming history, living environment, seasonal variation, and cohabitation with other animals were all assumed risk factors for BCoV infection ( p< 0.05). There was a significant ( p< 0.001) decrease in MCV (fl) and a significant ( p< 0.006) increase in TLC (1x10 3 /cm 3 ) on haematological analysis in BCoV-infected calves. TEC (1x10 6 /cm 3 ), WBC (1x10 6 /cm 3 ), RBC (1x10 6 /cm 3 ), and Hct% were significantly ( p< 0.05) higher. Similarly, infected calves had a non-significant ( p< 0.090) increase in K (mEq/L) and a significant ( p< 0.000) decrease in Na (mEq/L), Ca (mmol/L), Cu (mol/L), and Fe (mol/L) on biochemical analysis. It was concluded that the occurrence of BCoV infection was predisposed by assumed risk factors, and haemato-biochemical alterations were observed in BCoV infected calves.
牛冠状病毒(BCoV)是一种与新生牛犊呼吸道和肠道问题相关的主要病毒性病原体。本研究的目的是研究巴基斯坦Jhelum地区不同奶牛场和小家庭小牛BCoV感染的分子发生、血液生化变化以及与BCoV感染发生相关的危险因素。从2020年7月至2021年6月,从出现腹泻和痢疾症状的新生牛犊身上收集了200份粪便样本。使用S&C Biotech牛冠状病毒抗原快速检测试剂盒筛选样品,然后进行RT-PCR进行分子表征。为了进行血液生化分析,从感染BCoV的小牛身上采集血液。使用问卷收集与BCoV感染发生相关的危险因素信息。使用诊断筛查试剂盒检测到3.5%(7/200)的小牛感染BCoV,使用RT-PCR检测到3.0%(6/200)的小牛感染BCoV。品种、年龄、性别、呕吐、腹泻史、身体状况、食物类型、初乳摄食、驱虫史、生活环境、季节变化、与其他动物同居等均被认为是感染BCoV的危险因素(p< 0.05)。bcov感染犊牛血液学分析MCV (fl)显著(p< 0.001)降低,TLC (1 × 10 3 / cm3)显著(p< 0.006)升高。TEC (1 × 10 6 /cm 3)、WBC (1 × 10 6 /cm 3)、RBC (1 × 10 6 /cm 3)、Hct%显著升高(p< 0.05)。同样,感染犊牛的K (mEq/L)升高不显著(p< 0.090), Na (mEq/L)、Ca (mmol/L)、Cu (mol/L)和Fe (mol/L)降低显著(p< 0.000)。由此得出结论,BCoV感染的发生与假定的危险因素有关,并且在BCoV感染的小牛中观察到血液生化改变。
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引用次数: 0
Ameliorative Effect of Dietary Glutamine on Antioxidant Capacity of Broiler Chicks During Starter Phase Under Cold Stress 饲粮中添加谷氨酰胺对冷应激下肉鸡发酵期抗氧化能力的改善作用
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20221013091007
Wenfang Lou
1Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang, China 2College of Animal Science and Technology, Jiangxi Agriculture University, Jiangxi Province Key Laboratory of Animal Nutrition, Engineering Research Center of Nutritional Feed Development, Nanchang, China 3College of Animal Science, Anhui Science and Technology University, Fengyang, China 4College of Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan
1九江学院制药与生命科学系,九江2江西农业大学动物科学技术学院,江西省动物营养重点实验室,营养饲料开发工程研究中心,南昌3安徽科技大学动物科学学院,凤阳4农业大学兽医科学学院,白沙瓦
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引用次数: 0
Population Data and Internal Validation of the 21 Short Tandem Repeat Loci in Turkish Population 土耳其人群21个短串联重复位点的群体数据和内部验证
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20221013061003
Aatif Ahmad Khan
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引用次数: 0
Age and Growth of Triplophysa (Hedinichthys) yarkandensis (Day, 1877) in the Tarim River in Xinjiang, China 新疆塔里木河流域雅kandtriplophysa (Hedinichthys) yarkandensis (Day, 1877)的年龄和生长
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20221031021052
Xinyue Wang
Influenced by water pollution and flow
受水污染和水流影响
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引用次数: 0
Effect of Supplementation of Short-Fatty Acid on PER2 and Genes Related to Volatile Fatty Acid in Isolated Goat Ruminal Epithelial Tissue 补充短脂肪酸对山羊离体瘤胃上皮组织PER2及挥发性脂肪酸相关基因的影响
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20221101011143
Jinlong Cheng
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引用次数: 0
Morphological Description and Weight-Length Relationship of the Intertidal Sipuncula Siphonosoma australe (Keferstein, 1865) along the Central Coast of Vietnam 越南中部海岸潮间带Sipuncula Siphonosoma australe (Keferstein, 1865)的形态描述和重长关系
IF 0.6 4区 生物学 Q4 ZOOLOGY Pub Date : 2023-01-01 DOI: 10.17582/journal.pjz/20221110081129
Phuong-Thao Ho
1Laboratory of Ecology and Environmental Management, Science and Technology Advanced Institute, Van Lang University, Ho Chi Minh City, Vietnam 2Faculty of Applied Technology, School of Engineering and Technology, Van Lang University, Ho Chi Minh City, Vietnam 3Faculty of Chemistry-Biology-Environment, Pham Van Dong University, Quang Ngai City, 49000, Vietnam 4Biology Department, Hue University of Education, Hue University, 49000, Vietnam Article Information Received 10 November 2022 Revised 22 December 2022 Accepted 24 January 2023 Available online 22 May 2023 (early access)
1文朗大学科技高等研究院生态与环境管理实验室,越南胡志明市2文朗大学工程技术学院应用技术学院,越南胡志明市3范文同大学化学-生物-环境学院,越南广义市49000 4顺化教育大学生物系,顺化大学,49000越南文章信息收到2022年11月10日修订2022年12月22日接受2023年1月24日在线2023年5月22日(早期访问)
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引用次数: 0
期刊
Pakistan Journal of Zoology
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