Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20220324060344
H. Yasin
H.J
H.J
{"title":"Antinociceptive and Cytotoxic Effect of Extracts of Croton bonplandianus Leaves","authors":"H. Yasin","doi":"10.17582/journal.pjz/20220324060344","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220324060344","url":null,"abstract":"H.J","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67556317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20220411130406
Qun Lu
{"title":"Comparative Analysis of Otolith Micro-Characteristic in Schizothorax grahami and Spinibarbus sinensis","authors":"Qun Lu","doi":"10.17582/journal.pjz/20220411130406","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220411130406","url":null,"abstract":"","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67558225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20220523070520
S. Shah
The current study was conducted to assess the ovsynch estrus synchronization in imported pure Holstein Friesian and Holstein Friesian-Jersey crossbred cows at district Thatta Sindh. A total 30 Holstein Friesian and Holstein Friesian-Jersey crossbred cattle were selected and divided into three groups i.e., A (10 pure Holstein Friesian cows), B (10 Holstein Friesian-Jersey crossbred cows), and C (5 pure Holstein Friesian and 5 Holstein Friesian–Jersey crossbred cows) as control group. All selected animals were scanned through ultrasound to ensure the normality of reproductive tract. Animals of group A and group B were treated with Ovsynch protocol {GnRH; (Dalmarelin, FATRO) intramuscular at day 0 followed by PGF2α (Dalmazin; FATRO) intramuscular at day 7 and 2 nd GnRH intramuscular at day 9} for estrus synchronization while animals of group C was kept as untreated control (intramuscular injection of normal saline as placebo on day 0, 7 and 9). Animals of all group underwent fixed time insemination at 16 h of last treatment. Results showed that estrus response vary significantly among the groups (P<0.05). Overall estrus response was observed significantly higher in group B (80%), followed by A (60%) and C (10%). Intensity of estrus was determined by observing the estrus signs. Results showed significantly higher intensity of estrus signs in Group B than A and C for mucus discharge 90%, 65%,10%, flehmen 90%, 60%,10%, standing heat80%, 60%, 10% and sniffing vagina 80%, 70%, 10%, respectively. Estrus duration differs significantly among the treatment groups (P<0.05). Significantly maximum estrus duration was noted in group B (15.02 ± 0.21 h), then A (13.58 ± 2.22 h) and C (10 ± 0.00 h). In all groups blood samples were collected from the milk vein on day 0 (the first treatment), day 7, day 11 (one day after artificial insemination), day 18 and day 30 from all groups for determining progesterone level. Statistical analysis showed non-significant differences in progesterone level among groups at day 0. Furthermore, on Day 7, Group C differed significantly in progesterone level than A and B; however non-significant difference were observed in group A and B, while in group C difference was significant. On day 11 significantly highest values of blood progesterone were seen in group C (2.63 ± 0.22) followed by A (1.65 ± 0.35) and then B (1.48 ± 0.22). Statistical analysis showed that group C was significantly different from A and B, but there was non-significant difference between A and B. At day 18 highest P4 values were seen in B (4.20 ± 0.44) followed by A (3.33 ± 0.39) and then C (1.88 ± 0.59). Statistical analysis showed that group B and A had non-significant difference among each other, while both groups had significant differences with group C. Blood progesterone level for all the three groups on day 30 showed highest values in group B (10.90 ± 2.44) followed by A (8.28 ± 3.33) and C (4.53 ± 0.44). Statistical analysis showed significant difference am
{"title":"Evaluation of Ovsynch Estrus Synchronization in Holstein Friesian and Holstein Friesian-Jersey Crossbred Cows at District Thatta, Sindh, Pakistan","authors":"S. Shah","doi":"10.17582/journal.pjz/20220523070520","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220523070520","url":null,"abstract":"The current study was conducted to assess the ovsynch estrus synchronization in imported pure Holstein Friesian and Holstein Friesian-Jersey crossbred cows at district Thatta Sindh. A total 30 Holstein Friesian and Holstein Friesian-Jersey crossbred cattle were selected and divided into three groups i.e., A (10 pure Holstein Friesian cows), B (10 Holstein Friesian-Jersey crossbred cows), and C (5 pure Holstein Friesian and 5 Holstein Friesian–Jersey crossbred cows) as control group. All selected animals were scanned through ultrasound to ensure the normality of reproductive tract. Animals of group A and group B were treated with Ovsynch protocol {GnRH; (Dalmarelin, FATRO) intramuscular at day 0 followed by PGF2α (Dalmazin; FATRO) intramuscular at day 7 and 2 nd GnRH intramuscular at day 9} for estrus synchronization while animals of group C was kept as untreated control (intramuscular injection of normal saline as placebo on day 0, 7 and 9). Animals of all group underwent fixed time insemination at 16 h of last treatment. Results showed that estrus response vary significantly among the groups (P<0.05). Overall estrus response was observed significantly higher in group B (80%), followed by A (60%) and C (10%). Intensity of estrus was determined by observing the estrus signs. Results showed significantly higher intensity of estrus signs in Group B than A and C for mucus discharge 90%, 65%,10%, flehmen 90%, 60%,10%, standing heat80%, 60%, 10% and sniffing vagina 80%, 70%, 10%, respectively. Estrus duration differs significantly among the treatment groups (P<0.05). Significantly maximum estrus duration was noted in group B (15.02 ± 0.21 h), then A (13.58 ± 2.22 h) and C (10 ± 0.00 h). In all groups blood samples were collected from the milk vein on day 0 (the first treatment), day 7, day 11 (one day after artificial insemination), day 18 and day 30 from all groups for determining progesterone level. Statistical analysis showed non-significant differences in progesterone level among groups at day 0. Furthermore, on Day 7, Group C differed significantly in progesterone level than A and B; however non-significant difference were observed in group A and B, while in group C difference was significant. On day 11 significantly highest values of blood progesterone were seen in group C (2.63 ± 0.22) followed by A (1.65 ± 0.35) and then B (1.48 ± 0.22). Statistical analysis showed that group C was significantly different from A and B, but there was non-significant difference between A and B. At day 18 highest P4 values were seen in B (4.20 ± 0.44) followed by A (3.33 ± 0.39) and then C (1.88 ± 0.59). Statistical analysis showed that group B and A had non-significant difference among each other, while both groups had significant differences with group C. Blood progesterone level for all the three groups on day 30 showed highest values in group B (10.90 ± 2.44) followed by A (8.28 ± 3.33) and C (4.53 ± 0.44). Statistical analysis showed significant difference am","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67560362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20220629000640
Yongtao Xu
{"title":"Summer Diet of South China Sika Deer (Cervus nippon kopschi) in Taohongling based on High-Throughput Sequencing and Metabarcoding trnLkopschi) in Taohongling based on High-Throughput Sequencing and Metabarcoding trnL","authors":"Yongtao Xu","doi":"10.17582/journal.pjz/20220629000640","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220629000640","url":null,"abstract":"","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67562428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20220621070609
Perinçek Seçkinozan Şeker
For contributing to a better understanding of the phylogenetic position and evolutionary history of Turkish roe deer, mitochondrial DNA sequence data belonging to a population from Artvin, Turkey were introduced by this study. The cytochrome b (cyt b ) and cytochrome c oxidase subunit I (COI) genes of mitochondrial DNA belonging to the four samples of Capreolus capreolus were partially sequenced and analyzed alongside the sequences obtained from GenBank. The cyt b and COI data sets included sequences that were 1059 and 646 bp long, respectively. The Turkish roe deer population in Artvin had a relatively low nucleotide diversity and high haplotype diversity. A total of two haplotypes were identified for cyt b , while three haplotypes were identified for COI. Of the haplotypes detected, two cyt b and two COI haplotypes were specific to Turkey. Molecular phylogenetic analyses; Maximum Likelihood (ML), Bayesian Inference (BI), and Network revealed topologies and evolutionary networks which were not definitely resolved. Consequently, Turkish roe deer haplotypes were intertwined with the roe deer haplotypes from Central Europe instead of forming an independent evolutionary clade, and were also, found genetically close to Central European haplotypes. According to the evolutionary dating analysis, the divergence of C. capreolus populations in Europe started at the beginning of the Pleistocene (approximately 2.72 mya). This analysis also demonstrated that the divergence of Turkish roe deer began at approximately 0.58 mya. Given the existence of, shared haplotypes, the relative positions of Turkish roe deer haplotypes in the topologies of the ML and BI phylogenetic trees and evolutionary networks, and the times of evolutionary divergence events within this species; the following results may be inferred: (1) roe deer individuals with the same genotype as those in Europe can also be found in areas further east, such as Turkey; (2) these could be considered to be representatives, which may have contributed to the formation and shaping processes of the known admixed genetic structure of European roe deer populations in Europe; and finally, (3) the roe deer populations may have probably entered Turkey from Europe when evolutionary divergence times based on BI analysis are considered.
{"title":"Insight into the Phylogenetic Position of Capreolus capreolus, the European Roe Deer, (Mammalia: Cervidae) from Turkey using Mitochondrial Genes","authors":"Perinçek Seçkinozan Şeker","doi":"10.17582/journal.pjz/20220621070609","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220621070609","url":null,"abstract":"For contributing to a better understanding of the phylogenetic position and evolutionary history of Turkish roe deer, mitochondrial DNA sequence data belonging to a population from Artvin, Turkey were introduced by this study. The cytochrome b (cyt b ) and cytochrome c oxidase subunit I (COI) genes of mitochondrial DNA belonging to the four samples of Capreolus capreolus were partially sequenced and analyzed alongside the sequences obtained from GenBank. The cyt b and COI data sets included sequences that were 1059 and 646 bp long, respectively. The Turkish roe deer population in Artvin had a relatively low nucleotide diversity and high haplotype diversity. A total of two haplotypes were identified for cyt b , while three haplotypes were identified for COI. Of the haplotypes detected, two cyt b and two COI haplotypes were specific to Turkey. Molecular phylogenetic analyses; Maximum Likelihood (ML), Bayesian Inference (BI), and Network revealed topologies and evolutionary networks which were not definitely resolved. Consequently, Turkish roe deer haplotypes were intertwined with the roe deer haplotypes from Central Europe instead of forming an independent evolutionary clade, and were also, found genetically close to Central European haplotypes. According to the evolutionary dating analysis, the divergence of C. capreolus populations in Europe started at the beginning of the Pleistocene (approximately 2.72 mya). This analysis also demonstrated that the divergence of Turkish roe deer began at approximately 0.58 mya. Given the existence of, shared haplotypes, the relative positions of Turkish roe deer haplotypes in the topologies of the ML and BI phylogenetic trees and evolutionary networks, and the times of evolutionary divergence events within this species; the following results may be inferred: (1) roe deer individuals with the same genotype as those in Europe can also be found in areas further east, such as Turkey; (2) these could be considered to be representatives, which may have contributed to the formation and shaping processes of the known admixed genetic structure of European roe deer populations in Europe; and finally, (3) the roe deer populations may have probably entered Turkey from Europe when evolutionary divergence times based on BI analysis are considered.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67562527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20220701070727
Qurat-ul-Ain Ijaz
Qurat-ul-Ain Ijaz1, Amna Sulaman1, Dost Muhammad Baloch2, Muhammad Shafi2, Faiz Muhammad1* and Shahnaz Rashid1 1Center of Excellence in Marine Biology, University of Karachi, Karachi-75270, Pakistan 2Lasbella University of Agriculture, Water and Marine Sciences, Lasbella, Uthal Article Information Received 01 July 2022 Revised 15 August 2022 Accepted 14 September 2022 Available online 27 April 2023 (early access)
Qurat-ul-Ain Ijaz1, Amna Sulaman1, Dost Muhammad Baloch2, Muhammad Shafi2, Faiz Muhammad1*和Shahnaz Rashid1 1巴基斯坦卡拉奇-75270卡拉奇大学海洋生物学卓越中心2拉斯贝拉农业，水和海洋科学大学，拉斯贝拉，乌萨塔文章信息接收日期2022年7月1日修订2022年8月15日接受2022年9月14日2023年4月27日在线(提前获取)
{"title":"Identification of Nine Gastropod Species with Three New Records using Cytochrome Oxidase Sub-Unit 1 Marker","authors":"Qurat-ul-Ain Ijaz","doi":"10.17582/journal.pjz/20220701070727","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220701070727","url":null,"abstract":"Qurat-ul-Ain Ijaz1, Amna Sulaman1, Dost Muhammad Baloch2, Muhammad Shafi2, Faiz Muhammad1* and Shahnaz Rashid1 1Center of Excellence in Marine Biology, University of Karachi, Karachi-75270, Pakistan 2Lasbella University of Agriculture, Water and Marine Sciences, Lasbella, Uthal Article Information Received 01 July 2022 Revised 15 August 2022 Accepted 14 September 2022 Available online 27 April 2023 (early access)","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67563212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20221101011143
Jinlong Cheng
{"title":"Effect of Supplementation of Short-Fatty Acid on PER2 and Genes Related to Volatile Fatty Acid in Isolated Goat Ruminal Epithelial Tissue","authors":"Jinlong Cheng","doi":"10.17582/journal.pjz/20221101011143","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221101011143","url":null,"abstract":"","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67570121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20221011021005
Cheng-Hong Chen
.
．
{"title":"The Value of Procalcitonin Combined with C-Reactive Protein Assay in the Early Clinical Diagnosis of Bloodstream Infection","authors":"Cheng-Hong Chen","doi":"10.17582/journal.pjz/20221011021005","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221011021005","url":null,"abstract":".","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67567828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20221124101150
M. Muneeb
Aquatic resources are being devastating rapidly due to the continuous intrusion of untreated wastes into the environment due to rapid industrialization and causing severe problems to aquatic life. Different physicochemical methods have been used to reduce these pollutants but all have their own limitations including production of secondary pollutants. The current study was designed to show the effect of cellulolytic and sulphate-reducing bacterial species in the form of a co-culture to treat an in-vitro prepared sulphate-rich wastewater while employing various agro-industrial organic waste as economical growth substrates. A combination of sulphate-reducing and cellulolytic bacteria in a ratio of 1:1 (v/v) was proved to be efficient for the reduction of sulphate in controlled as well as in the experimental conditions. The implicated microbial co-culture reduced 96 and 93 % of the added sulphate (5 gL −1 ) while using rice straw and animal manure, respectively in a 60-day trial of anaerobic incubation. Mixture of industrial and agricultural waste reduced about 90 % of the total added sulphate. A trend of decrease in pH with time was observed in all the incubated cultures. Our findings will be helpful for devising sustainable waste management strategies.
{"title":"Efficient Sulphate Reduction by Cellulolytic and Sulphate-Reducing Bacterial Co-Culture Using Different Agro-Industrial Wastes as Growth Substrates","authors":"M. Muneeb","doi":"10.17582/journal.pjz/20221124101150","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221124101150","url":null,"abstract":"Aquatic resources are being devastating rapidly due to the continuous intrusion of untreated wastes into the environment due to rapid industrialization and causing severe problems to aquatic life. Different physicochemical methods have been used to reduce these pollutants but all have their own limitations including production of secondary pollutants. The current study was designed to show the effect of cellulolytic and sulphate-reducing bacterial species in the form of a co-culture to treat an in-vitro prepared sulphate-rich wastewater while employing various agro-industrial organic waste as economical growth substrates. A combination of sulphate-reducing and cellulolytic bacteria in a ratio of 1:1 (v/v) was proved to be efficient for the reduction of sulphate in controlled as well as in the experimental conditions. The implicated microbial co-culture reduced 96 and 93 % of the added sulphate (5 gL −1 ) while using rice straw and animal manure, respectively in a 60-day trial of anaerobic incubation. Mixture of industrial and agricultural waste reduced about 90 % of the total added sulphate. A trend of decrease in pH with time was observed in all the incubated cultures. Our findings will be helpful for devising sustainable waste management strategies.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67571744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20221128091109
Haigang Wu
.,
.，
{"title":"Effects of Chinese Herbal Medicine Additives on Laying Performance, Egg Quality and Yolk Nutrition of Laying Hens During Late Laying Period","authors":"Haigang Wu","doi":"10.17582/journal.pjz/20221128091109","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221128091109","url":null,"abstract":".,","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67572080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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