Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20220812000812
M. K. A. el-Megid
.
．
{"title":"Evaluation of Anti-Diabetic Activity of Aqueous Extract of Gamma Irradiated Sesame Seeds","authors":"M. K. A. el-Megid","doi":"10.17582/journal.pjz/20220812000812","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220812000812","url":null,"abstract":".","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"31 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67565715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20220823110833
T. Bozlar
This study aimed to determine the effect of spatial and climatic factors on the productivity of chestnut honey and chestnut propolis produced in chestnut forests and to reveal the potential contribution of their production to the economy. The study area included the provinces of Artvin, Trabzon, Ordu and Samsun in the Eastern and Central Black Sea Region, and covered the beekeepers producing chestnut honey and chestnut propolis in chestnut forests. The primary data of the study were obtained from the yield measurements of chestnut honey and chestnut propolis obtained from the hives, whereas the secondary data were obtained from the surveys conducted with beekeepers. Analysis of variance, independent sample t-test and correlation analysis were used to analyze the statistical difference between the chestnut honey and chestnut propolis yields of the beehives in different provinces. It was determined that there was a negative relationship between the yield of chestnut honey and chestnut propolis, relative humidity and wind speed, and a positive relationship between yield and altitude, temperature and precipitation. The potential contribution of chestnut honey and propolis production was US $ 51.10 million to the Eastern and Central Black Sea Region economy.
{"title":"Spatial and Climatic Analysis of Chestnut Honey and Propolis Produced in Chestnut Forests and Their Potential Contribution to the Economy","authors":"T. Bozlar","doi":"10.17582/journal.pjz/20220823110833","DOIUrl":"https://doi.org/10.17582/journal.pjz/20220823110833","url":null,"abstract":"This study aimed to determine the effect of spatial and climatic factors on the productivity of chestnut honey and chestnut propolis produced in chestnut forests and to reveal the potential contribution of their production to the economy. The study area included the provinces of Artvin, Trabzon, Ordu and Samsun in the Eastern and Central Black Sea Region, and covered the beekeepers producing chestnut honey and chestnut propolis in chestnut forests. The primary data of the study were obtained from the yield measurements of chestnut honey and chestnut propolis obtained from the hives, whereas the secondary data were obtained from the surveys conducted with beekeepers. Analysis of variance, independent sample t-test and correlation analysis were used to analyze the statistical difference between the chestnut honey and chestnut propolis yields of the beehives in different provinces. It was determined that there was a negative relationship between the yield of chestnut honey and chestnut propolis, relative humidity and wind speed, and a positive relationship between yield and altitude, temperature and precipitation. The potential contribution of chestnut honey and propolis production was US $ 51.10 million to the Eastern and Central Black Sea Region economy.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"50 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67566192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20221013201049
Khalid Mahmood
The family Nimravidae is poorly known from the Siwalik Group that comprises freshwater deposits having an age that spans 18.0-0.6 Ma. The new material recovered from the Dhok Ban Ameer Khatoon (Chinji Formation) and Sethi Nagri (type locality of the Nagri Formation) is assigned to Sansanosmilus rhomboidalis based on the morphology of canine. The recovered material is unique and rare in the Siwaliks of northern Pakistan. It also increases the stratigraphic range of this barbourofelines species from the Chinji Formation to the Nagri Formation.
Nimravidae家族在Siwalik组中鲜为人知，该组由年龄为18.0-0.6 Ma的淡水矿床组成。在Dhok Ban Ameer Khatoon (Chinji组)和Sethi Nagri (Nagri组的模式地点)中发现的新材料根据犬类的形态归属于Sansanosmilus rhomboidalis。这种回收的材料在巴基斯坦北部的siwalik地区是独一无二的。这也增加了从Chinji组到Nagri组这一有刺猫科动物物种的地层范围。
{"title":"Barbourofelines from the Middle-Late Miocene of the Siwaliks, Pakistan","authors":"Khalid Mahmood","doi":"10.17582/journal.pjz/20221013201049","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221013201049","url":null,"abstract":"The family Nimravidae is poorly known from the Siwalik Group that comprises freshwater deposits having an age that spans 18.0-0.6 Ma. The new material recovered from the Dhok Ban Ameer Khatoon (Chinji Formation) and Sethi Nagri (type locality of the Nagri Formation) is assigned to Sansanosmilus rhomboidalis based on the morphology of canine. The recovered material is unique and rare in the Siwaliks of northern Pakistan. It also increases the stratigraphic range of this barbourofelines species from the Chinji Formation to the Nagri Formation.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67568710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20221017071041
Chundan Shi
This animal study aimed to investigate the regulatory effect of FAM209, and PICK1protein in SD rats with Rodent pest. SD rats are randomly divided into control group, low (10 mg/kg of plant complex sterility agent) and high dose (20 mg/kg of plant complex sterility agent) groups. Hematoxylin and eosin staining is used to observe the pathological changes of testis tissue. The testis organ index, male seminal vesicle, and sperm were also detected. The expression of FAM209, and PICK1protein in testis tissue are detected by Western blotting. Compared with the control group, the cell structure in the testis tissue of the model group is damaged, and testis organ index, male seminal vesicle, the sperm density level in the control group, low dose group are significantly increased ( P <0.05). The results of WB confirm the expression of FAM209 ( P <0.05). downregulated of FAM209, and PICK1protein may be involved in the development of rodent pest.
{"title":"The Role of Plant Complex Sterility Agent in Rats via FAM209 Regulating PICK1","authors":"Chundan Shi","doi":"10.17582/journal.pjz/20221017071041","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221017071041","url":null,"abstract":"This animal study aimed to investigate the regulatory effect of FAM209, and PICK1protein in SD rats with Rodent pest. SD rats are randomly divided into control group, low (10 mg/kg of plant complex sterility agent) and high dose (20 mg/kg of plant complex sterility agent) groups. Hematoxylin and eosin staining is used to observe the pathological changes of testis tissue. The testis organ index, male seminal vesicle, and sperm were also detected. The expression of FAM209, and PICK1protein in testis tissue are detected by Western blotting. Compared with the control group, the cell structure in the testis tissue of the model group is damaged, and testis organ index, male seminal vesicle, the sperm density level in the control group, low dose group are significantly increased ( P <0.05). The results of WB confirm the expression of FAM209 ( P <0.05). downregulated of FAM209, and PICK1protein may be involved in the development of rodent pest.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67569187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20221105001122
W. Xiong
langsoni , Pseudohemiculter dispar , Ptychidio jordani , and Cranoglanis bouderius ), qualify for recognition as Threatened Species. Overfishing, water flow diversions and modifications, and the impacts of non-native species are the greatest threats to freshwater fish biodiversity in the Yujiang River. We recommend the adoption and enforcement of additional protected areas, improvement in approaches to sustainable fishery management, much better control of non-native species, and an improvement in and the expansion of fish life-history research. Our study contributes recommendations for the better protection of freshwater fish biodiversity and the development of sustainable fisheries in the Yujiang River.
{"title":"Biodiversity and Conservation of Freshwater Fishes in the Yujiang River, China","authors":"W. Xiong","doi":"10.17582/journal.pjz/20221105001122","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221105001122","url":null,"abstract":"langsoni , Pseudohemiculter dispar , Ptychidio jordani , and Cranoglanis bouderius ), qualify for recognition as Threatened Species. Overfishing, water flow diversions and modifications, and the impacts of non-native species are the greatest threats to freshwater fish biodiversity in the Yujiang River. We recommend the adoption and enforcement of additional protected areas, improvement in approaches to sustainable fishery management, much better control of non-native species, and an improvement in and the expansion of fish life-history research. Our study contributes recommendations for the better protection of freshwater fish biodiversity and the development of sustainable fisheries in the Yujiang River.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67569976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20221223091220
Xiaowei Zhang
The objective of this study was to investigate the effect of two chemotherapeutic drugs
本研究的目的是探讨两种化疗药物的疗效
{"title":"Clinical Effect of Apartinib Combined with Trastuzumab on Gastric Cancer with Ascites","authors":"Xiaowei Zhang","doi":"10.17582/journal.pjz/20221223091220","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221223091220","url":null,"abstract":"The objective of this study was to investigate the effect of two chemotherapeutic drugs","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67572410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20221229071222
M. Shahid
Vaccine failure against infectious bronchitis (IB) due to emergence of nephro-pathogenic strains in field is a major problem. This study evaluated cross protection of commercial IBV vaccines against field isolates. A total of 160, day-old chicks were equally divided into four groups. Group A was vaccinated with a single dose of H120 strain IBV vaccine, group B with two doses (days 02 and 15) of H120 strain, group C with two doses of heterologous strains (H120 on day 02 and 4/91 on 15) while group D kept unvaccinated. Antibody titer was evaluated using a commercial ELISA kit. The difference in antibody titer of vaccinated groups was non-significant (P value > 0.05) till two weeks post-vaccination. At 04 weeks of age, the highest antibody titer was observed for group B, followed by group C and A. Challenge with a field isolate of IBV induced a rise in antibody titer in all groups. Group D had the highest score (severe) of clinical signs and mortality (n=04/10) followed by group A, showing a moderate score of clinical signs and mortality (n=02/10). In contrast, group C was the most protected group showing mild signs and no mortality. Nephro-pathogenic gross lesions were predominant in all groups except group C which had the lowest score. It was concluded that nephro-pathogenic strains are involved in field outbreaks of IB. Further, a vaccination program only with a classical strain could not provide full protection while priming with a classical strain in first week and boosting by a variant strain vaccine after second week may provide better protection against the disease. Protective-typing is also recommended for development of vaccine from locally isolated strains.
{"title":"An Analysis of Cross-Protection of Commercial IBV Vaccines against Locally Isolated Field Strains of Infectious Bronchitis Virus in Pakistan","authors":"M. Shahid","doi":"10.17582/journal.pjz/20221229071222","DOIUrl":"https://doi.org/10.17582/journal.pjz/20221229071222","url":null,"abstract":"Vaccine failure against infectious bronchitis (IB) due to emergence of nephro-pathogenic strains in field is a major problem. This study evaluated cross protection of commercial IBV vaccines against field isolates. A total of 160, day-old chicks were equally divided into four groups. Group A was vaccinated with a single dose of H120 strain IBV vaccine, group B with two doses (days 02 and 15) of H120 strain, group C with two doses of heterologous strains (H120 on day 02 and 4/91 on 15) while group D kept unvaccinated. Antibody titer was evaluated using a commercial ELISA kit. The difference in antibody titer of vaccinated groups was non-significant (P value > 0.05) till two weeks post-vaccination. At 04 weeks of age, the highest antibody titer was observed for group B, followed by group C and A. Challenge with a field isolate of IBV induced a rise in antibody titer in all groups. Group D had the highest score (severe) of clinical signs and mortality (n=04/10) followed by group A, showing a moderate score of clinical signs and mortality (n=02/10). In contrast, group C was the most protected group showing mild signs and no mortality. Nephro-pathogenic gross lesions were predominant in all groups except group C which had the lowest score. It was concluded that nephro-pathogenic strains are involved in field outbreaks of IB. Further, a vaccination program only with a classical strain could not provide full protection while priming with a classical strain in first week and boosting by a variant strain vaccine after second week may provide better protection against the disease. Protective-typing is also recommended for development of vaccine from locally isolated strains.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67572881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20230119060159
M. Shahid
The chronic hepatitis C (CHC) virus plays a vital role around the globe in hepatic persistent disease. It is undoubtedly a major health and financial burden in Pakistan, affecting about 6 to 10 percent of the overall population. The purpose of this research is to identify the baseline clinical and virological variables to predict the therapy outcomes. In this study, a total of 118 confirmed CHC-positive patients were selected based on the inclusion criteria. The HCV genotype and virus titer of the selected patients were measured. All patients received pegylated interferon with the addition of ribavirin. Analysis of variance (ANOVA) has been used for comparing variations in clinical data between patient groups. The clinical and virological data have been analyzed using correlation analysis. For the assessment of therapy response in patients, a binary logistic regression model has been used. ANOVA showed that differences in albumin %, AST/ALT ratio, and monocyte % between responders and non-responders have been statistically significant (P < 0.05). In the predictive analysis, three variables γ-GT, globulin, and albumin were predictors of treatment. Our study showed that clinical and virological parameters can be used as a useful tool for predicting treatment outcomes. These parameters can be used to accurate predictions of the Pakistani population in patients with newly diagnosed HCV-3a.
{"title":"A Comparative Analysis of Baseline Variables in Responder and Non-Responder Groups of Chronic Hepatitis C patients","authors":"M. Shahid","doi":"10.17582/journal.pjz/20230119060159","DOIUrl":"https://doi.org/10.17582/journal.pjz/20230119060159","url":null,"abstract":"The chronic hepatitis C (CHC) virus plays a vital role around the globe in hepatic persistent disease. It is undoubtedly a major health and financial burden in Pakistan, affecting about 6 to 10 percent of the overall population. The purpose of this research is to identify the baseline clinical and virological variables to predict the therapy outcomes. In this study, a total of 118 confirmed CHC-positive patients were selected based on the inclusion criteria. The HCV genotype and virus titer of the selected patients were measured. All patients received pegylated interferon with the addition of ribavirin. Analysis of variance (ANOVA) has been used for comparing variations in clinical data between patient groups. The clinical and virological data have been analyzed using correlation analysis. For the assessment of therapy response in patients, a binary logistic regression model has been used. ANOVA showed that differences in albumin %, AST/ALT ratio, and monocyte % between responders and non-responders have been statistically significant (P < 0.05). In the predictive analysis, three variables γ-GT, globulin, and albumin were predictors of treatment. Our study showed that clinical and virological parameters can be used as a useful tool for predicting treatment outcomes. These parameters can be used to accurate predictions of the Pakistani population in patients with newly diagnosed HCV-3a.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67573092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20230206110216
Anum Razzaq
The Indian gerbil Tatera indica occurs in the Pothwar Plateau and is associated with agro-ecosystem of the area. No particular scientific studies have been focussed on dietary habits of this species in particular Plateau. Therefore, the focal aim of the current study was to investigate the dietary habits of Indian gerbil by using micro-histological analysis of stomach contents in croplands of the Pothwar Plateau and to study the variation in its food composition during cropping and non-cropping seasons. A total of 30 specimens were trapped by using snap traps in wheat, groundnut and adjacent non-crop habitats. Results showed that the gerbil rat was chiefly omnivore; feeding on wheat-groundnut plants and grains as the crops approached maturity, wild flora (viz: herbs, grasses, seeds and tubers) invariably amongst crop stages and seasons. During spring season, wheat was the most frequently consumed cereal. But during winter, as groundnut crop approached towards maturity/harvested, gerbils consumed mainly nuts and grains, while in autumn and summer (non-crop periods), the rat species switched its diet to wild flora, and consumed most frequently Ziziphus nummularia (Beri) followed by Cynodon dactylon (Khabbal grass), Desmostachya bipinnate (Baron dhab), Artemisia dubia etc. along with some fodder crops like Sorghum bicolour (Sorghum), Zea mays (Maize), Brassica campestris (Mustard) etc. The summer diet (non-crop season) was comparatively less diversified than the diet of the cropping season (spring) and there was a significant difference in the diets of this rat species during cropping and non-cropping period. The gerbil also supplemented its diet with insects (animal matters) in higher quantities highlighting its positive role as biological control of insect pests of croplands. Analysis of Variance (ANOVA) later followed by Least Significance Difference (LSD) revealed significant differences during cropping season winter (wheat) and non-cropping season autumn (F = 1.88, df = 18, P < 0.05). The frequency of different food items of cropping and non-cropping season (summer and monsoon) showed the significant difference (F = 1.15, df = 18, P < 0.05) and the remaining unidentified food items were non-significant to each other (F = 1.10, df = 18, P > 0.05). The study concludes that Indian gerbil has got a beneficial role for sustainable agriculture practices.
{"title":"Dietary Habits of Indian Gerbil Tatera indica Inhabiting Agro-Ecosystem of Pothwar Plateau, Pakistan","authors":"Anum Razzaq","doi":"10.17582/journal.pjz/20230206110216","DOIUrl":"https://doi.org/10.17582/journal.pjz/20230206110216","url":null,"abstract":"The Indian gerbil Tatera indica occurs in the Pothwar Plateau and is associated with agro-ecosystem of the area. No particular scientific studies have been focussed on dietary habits of this species in particular Plateau. Therefore, the focal aim of the current study was to investigate the dietary habits of Indian gerbil by using micro-histological analysis of stomach contents in croplands of the Pothwar Plateau and to study the variation in its food composition during cropping and non-cropping seasons. A total of 30 specimens were trapped by using snap traps in wheat, groundnut and adjacent non-crop habitats. Results showed that the gerbil rat was chiefly omnivore; feeding on wheat-groundnut plants and grains as the crops approached maturity, wild flora (viz: herbs, grasses, seeds and tubers) invariably amongst crop stages and seasons. During spring season, wheat was the most frequently consumed cereal. But during winter, as groundnut crop approached towards maturity/harvested, gerbils consumed mainly nuts and grains, while in autumn and summer (non-crop periods), the rat species switched its diet to wild flora, and consumed most frequently Ziziphus nummularia (Beri) followed by Cynodon dactylon (Khabbal grass), Desmostachya bipinnate (Baron dhab), Artemisia dubia etc. along with some fodder crops like Sorghum bicolour (Sorghum), Zea mays (Maize), Brassica campestris (Mustard) etc. The summer diet (non-crop season) was comparatively less diversified than the diet of the cropping season (spring) and there was a significant difference in the diets of this rat species during cropping and non-cropping period. The gerbil also supplemented its diet with insects (animal matters) in higher quantities highlighting its positive role as biological control of insect pests of croplands. Analysis of Variance (ANOVA) later followed by Least Significance Difference (LSD) revealed significant differences during cropping season winter (wheat) and non-cropping season autumn (F = 1.88, df = 18, P < 0.05). The frequency of different food items of cropping and non-cropping season (summer and monsoon) showed the significant difference (F = 1.15, df = 18, P < 0.05) and the remaining unidentified food items were non-significant to each other (F = 1.10, df = 18, P > 0.05). The study concludes that Indian gerbil has got a beneficial role for sustainable agriculture practices.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67573908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.17582/journal.pjz/20230228180245
Sehar Aslam
Probiotics are live microbes that offer potential health benefits to the host, including modulation of the host immune system, improvement of anti-inflammatory response, enhancement of antibacterial and anti-allergic properties, as well as anti-proliferative properties. These beneficial microorganisms can interact with the gut microbiota to restore an impaired gut microbiome. While probiotics can be sourced from various sources, this study focuses on isolating and characterizing novel probiotic strains from dairy sources, specifically Dahi. To evaluate the probiotic potential of these isolates, various biochemical, morphological, and physiological tests were performed, followed by 16S rRNA gene sequencing to genotype the isolates. The tolerance of the isolates to pH, temperature, and bile salt, as well as their antimicrobial and adhesion ability, was evaluated. The results showed that the identified novel probiotic strains belonged to the Lactobacillus fermentum species, exhibited remarkable tolerance against bile salts, acidic environments, and temperature, and had excellent adhesion ability, indicating their potential as probiotic strains. Additionally, all isolates were non-hemolytic and displayed significant antimicrobial activity against antibiotic-resistant pathogens, such as Escherichia coli and Staphylococcus aureus . The anti-cancer activity of all isolates was also evaluated against the Human colorectal adenocarcinoma cell line (Caco-2), and all isolates showed significant anticancer activity. These findings validate the beneficial therapeutic values of novel probiotic strains isolated from dairy sources, specifically Dahi, and suggest that they could be used in food and drugs to treat various diseases.
{"title":"Exploring the Probiotic Potential and Safety of a Locally Sourced Lactobacillus fermentum Strain Isolated from Dahi, a Traditional Dairy Product","authors":"Sehar Aslam","doi":"10.17582/journal.pjz/20230228180245","DOIUrl":"https://doi.org/10.17582/journal.pjz/20230228180245","url":null,"abstract":"Probiotics are live microbes that offer potential health benefits to the host, including modulation of the host immune system, improvement of anti-inflammatory response, enhancement of antibacterial and anti-allergic properties, as well as anti-proliferative properties. These beneficial microorganisms can interact with the gut microbiota to restore an impaired gut microbiome. While probiotics can be sourced from various sources, this study focuses on isolating and characterizing novel probiotic strains from dairy sources, specifically Dahi. To evaluate the probiotic potential of these isolates, various biochemical, morphological, and physiological tests were performed, followed by 16S rRNA gene sequencing to genotype the isolates. The tolerance of the isolates to pH, temperature, and bile salt, as well as their antimicrobial and adhesion ability, was evaluated. The results showed that the identified novel probiotic strains belonged to the Lactobacillus fermentum species, exhibited remarkable tolerance against bile salts, acidic environments, and temperature, and had excellent adhesion ability, indicating their potential as probiotic strains. Additionally, all isolates were non-hemolytic and displayed significant antimicrobial activity against antibiotic-resistant pathogens, such as Escherichia coli and Staphylococcus aureus . The anti-cancer activity of all isolates was also evaluated against the Human colorectal adenocarcinoma cell line (Caco-2), and all isolates showed significant anticancer activity. These findings validate the beneficial therapeutic values of novel probiotic strains isolated from dairy sources, specifically Dahi, and suggest that they could be used in food and drugs to treat various diseases.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67574546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：