Pub Date : 2013-01-01DOI: 10.3724/SP.J.1142.2013.40378
F. Dang, Y. Lei, D. Guan, Zaiteng Wang, Shuilin He
{"title":"Identification and evaluation of resistance to bacterial wilt in pepper","authors":"F. Dang, Y. Lei, D. Guan, Zaiteng Wang, Shuilin He","doi":"10.3724/SP.J.1142.2013.40378","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.40378","url":null,"abstract":"","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"378"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69788694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1142.2013.40415
Jianwei Ban, Jia Feng, S. Xie
This study attempted to choose potential oil-producing microalgae candidates across Shanxi province.Thirty-two strains of microalgae were tested.Among these microalgae,29 strains were isolated from water samples collected from four sites in the Fenghe River and the Salt Lakes Area in Yuncheng.Three strains of microalgae were bought from the Freshwater Algae Culture Collection of the Institute of Hydrobiology.Microalgae from the Shanxi province were isolated and purified by Micro-picking method and Spread-plate method.The biomass of 32 strains of microalgae varied between 48.9-422.2 mg/L,which were determined by dry weight.Chloroform-methanol extraction is a traditional total lipid measuring method and Nile red fluorescence is a new technique used for determining neutral lipids.In this study,Chloroform-methanol extraction and Nile red fluorescent staining were combined to determine lipid contents of the 32 strains of microalgae.The results showed that total lipid content of all strains were in the range of 5.4% DW-30.1% DW,and fluorescence value in unit volume were in the range of 4.1-181.5.High lipid productivity is a key selection criterion of species for biodiesel production.According to the lipid productivity of the microalgae,we screened three strains of microalgae that may be potential oil-producing candidates in Shanxi province.These three strains included NY017(Dunaliella salina),NY023(Nitzschia linearis)and NY025(Nitzschia palea).Their lipid productivities were 3.25 mg · L-1 · d-1,3.03 mg · L-1 · d and 2.11 mg · L-1 · d-1 respectively.
{"title":"Isolation and Screening of Microalgae with High-lipid Contents in Shanxi Province","authors":"Jianwei Ban, Jia Feng, S. Xie","doi":"10.3724/SP.J.1142.2013.40415","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.40415","url":null,"abstract":"This study attempted to choose potential oil-producing microalgae candidates across Shanxi province.Thirty-two strains of microalgae were tested.Among these microalgae,29 strains were isolated from water samples collected from four sites in the Fenghe River and the Salt Lakes Area in Yuncheng.Three strains of microalgae were bought from the Freshwater Algae Culture Collection of the Institute of Hydrobiology.Microalgae from the Shanxi province were isolated and purified by Micro-picking method and Spread-plate method.The biomass of 32 strains of microalgae varied between 48.9-422.2 mg/L,which were determined by dry weight.Chloroform-methanol extraction is a traditional total lipid measuring method and Nile red fluorescence is a new technique used for determining neutral lipids.In this study,Chloroform-methanol extraction and Nile red fluorescent staining were combined to determine lipid contents of the 32 strains of microalgae.The results showed that total lipid content of all strains were in the range of 5.4% DW-30.1% DW,and fluorescence value in unit volume were in the range of 4.1-181.5.High lipid productivity is a key selection criterion of species for biodiesel production.According to the lipid productivity of the microalgae,we screened three strains of microalgae that may be potential oil-producing candidates in Shanxi province.These three strains included NY017(Dunaliella salina),NY023(Nitzschia linearis)and NY025(Nitzschia palea).Their lipid productivities were 3.25 mg · L-1 · d-1,3.03 mg · L-1 · d and 2.11 mg · L-1 · d-1 respectively.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"415"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69788930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1142.2013.60545
Lai Guang
Based on complex observation of type materials, real photos and collections, investigation of type locality and examination of original descriptions, some taxonomically uncertain Latin names under Phyllostachys Sieb. et Zucc. were discussed and verified. As a result,P. sapida Yi( = P. arcana McClure),P. balansae Nguyen( = P. bambusoides Sieb. et Zucc.),P. subulata W. T. Lin et Z. M. Wu( = P. bambusoides Sieb. et Zucc. f. shouzhu Yi),P. cantoniensis W. T. Lin( = P. heteroclada Oliv.),P. lofushanensis C. P. Wang et al.( = P. nidularia Munro f. basipilis G. H. Lai),P. aristata W. T. Lin( = P. nidularia Munro f. basipilis G. H. Lai), P. guizhouensis C. S. Chao et J. Q. Zhang( = P. nigra( Lodd. ex Lindl.) Munro var. henonis( Mitf.) Stapf ex Rendle), and P. lithophila Hayata( = P. sulphurea( Carr.) A. et C. Riv. var. viridis R. A. Young) were all reduced to synonyms; the systematic position of three species from Vietnam was proposed: P. caobangensis Nguyen may possibly be conspecific with P. bambusoides Sieb. et Zucc., and P. baccanensis Nguyen and P. vietbacensis Nguyen conspecific with P. kwangsiensis W. Y. Hsiung et al.
{"title":"A Revision of Some Species of the GenusPhyllostachys(Gramineae—Bambusoideae)(V)","authors":"Lai Guang","doi":"10.3724/SP.J.1142.2013.60545","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.60545","url":null,"abstract":"Based on complex observation of type materials, real photos and collections, investigation of type locality and examination of original descriptions, some taxonomically uncertain Latin names under Phyllostachys Sieb. et Zucc. were discussed and verified. As a result,P. sapida Yi( = P. arcana McClure),P. balansae Nguyen( = P. bambusoides Sieb. et Zucc.),P. subulata W. T. Lin et Z. M. Wu( = P. bambusoides Sieb. et Zucc. f. shouzhu Yi),P. cantoniensis W. T. Lin( = P. heteroclada Oliv.),P. lofushanensis C. P. Wang et al.( = P. nidularia Munro f. basipilis G. H. Lai),P. aristata W. T. Lin( = P. nidularia Munro f. basipilis G. H. Lai), P. guizhouensis C. S. Chao et J. Q. Zhang( = P. nigra( Lodd. ex Lindl.) Munro var. henonis( Mitf.) Stapf ex Rendle), and P. lithophila Hayata( = P. sulphurea( Carr.) A. et C. Riv. var. viridis R. A. Young) were all reduced to synonyms; the systematic position of three species from Vietnam was proposed: P. caobangensis Nguyen may possibly be conspecific with P. bambusoides Sieb. et Zucc., and P. baccanensis Nguyen and P. vietbacensis Nguyen conspecific with P. kwangsiensis W. Y. Hsiung et al.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"545"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69789411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1142.2013.50485
Yangchen Yuan, Qing‐Feng Wang, Jin‐Ming Chen
Using the program MISA,a total of 12319EST-SSRs were sought out from 79536ESTs of aquatic plant Nymphoides peltata.Dinucleotide and trinucleotide SSRs were the two main motif types,accounting for 57.31% and 30.87%,respectively.The most abundant di-and tri-motifs were AG/CT(29.76%)and AAG/CTT(8.66%),respectively.We randomly selected 130primer pairs to evaluate their application and polymorphism across two populations of N.peltata.Seventyeight SSR markers were amplified successfully,yielding clear and discernible bands,among which37were found to be polymorphic and the polymorphic rate was 47.44%.Using the polymorphic SSR markers,a total of 114alleles were detected.The number of alleles per locus ranged from 2to 6(mean 3.08).Observed heterozygosity(Ho)and expected value(He)per locus varied from 0.229to 1.000and from 0.351to 0.756,respectively.Polymorphism information content(PIC)ranged from 0.286to 0.698,with an average of 0.495.The results indicated that the development of SSR molecular markers from the EST dataset generated by next generation sequencing in the aquatic plant N.peltata is valuable and effective.These newly generated SSR makers will provide new tools for studying genetic diversity and population genetic structure of N. peltata.
{"title":"Development of SSR Markers in Aquatic Plant Nymphoides peltata( Menyanthaceae ) Based on Information from Transcriptome Sequencing","authors":"Yangchen Yuan, Qing‐Feng Wang, Jin‐Ming Chen","doi":"10.3724/SP.J.1142.2013.50485","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.50485","url":null,"abstract":"Using the program MISA,a total of 12319EST-SSRs were sought out from 79536ESTs of aquatic plant Nymphoides peltata.Dinucleotide and trinucleotide SSRs were the two main motif types,accounting for 57.31% and 30.87%,respectively.The most abundant di-and tri-motifs were AG/CT(29.76%)and AAG/CTT(8.66%),respectively.We randomly selected 130primer pairs to evaluate their application and polymorphism across two populations of N.peltata.Seventyeight SSR markers were amplified successfully,yielding clear and discernible bands,among which37were found to be polymorphic and the polymorphic rate was 47.44%.Using the polymorphic SSR markers,a total of 114alleles were detected.The number of alleles per locus ranged from 2to 6(mean 3.08).Observed heterozygosity(Ho)and expected value(He)per locus varied from 0.229to 1.000and from 0.351to 0.756,respectively.Polymorphism information content(PIC)ranged from 0.286to 0.698,with an average of 0.495.The results indicated that the development of SSR molecular markers from the EST dataset generated by next generation sequencing in the aquatic plant N.peltata is valuable and effective.These newly generated SSR makers will provide new tools for studying genetic diversity and population genetic structure of N. peltata.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"485"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69789595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1142.2013.30228
Fengqi Li, L. Kong, Yusheng Liu, He Wang, Jun-hua Peng
{"title":"TOPSIS Based Comprehensive Evaluation of the Resistance in Wheat Germplasm to English Grain Aphid","authors":"Fengqi Li, L. Kong, Yusheng Liu, He Wang, Jun-hua Peng","doi":"10.3724/SP.J.1142.2013.30228","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.30228","url":null,"abstract":"","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"228"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69788319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1142.2013.50500
Qiu Daoshou, Xi-Long Zheng, Cai Shike, Jin-Rong Zheng, Lu Huanming, Zhang Lei, Deng Rui-yun, Wu Li, Liu Xiaojin
To date,only 100SSR markers in Dendrobiumhave been developed,which are far from sufficient for research applications.To develop molecular markers,we mined SSR of Dendrobium from public nucleotide data through bioinformation methods.Some 1343Uni-DNA sequences were assembled from the 3599DNA sequences of Dendrobiumfrom GenBank.By scanning the Uni-DNA sequences,283SSRs were distributed in 205Uni-DNA sequences,with an average frequency of 1SSR per 2815bp.Sequence alignment indicated that 86of the 205SSR-DNA sequences had already been used to design primers.In this study,76primer pairs were designed from the remaining 119sequences for transferability analysis among 32 Dendrobium species.Results showed that 47primer pairs were amplified effectively with transfer rates ranging from 51.1%to 95.7%(average75.9%).Of which,46primer pairs were able to detect polymorphism among the Dendrobium species with 2-8alleles(average 4.0alleles).Ten pairs of polymorphic primers were selected to detect polymorphism in 60accessions of D.officinale,and 2-5alleles(average 3.4alleles)were found per SSR locus.Based on the SSR amplification pattern,the 60accessions of D.officinale were clustered into five clusters,and phenotypes were closer within clusters than between clusters.The sequencing of the amplified fragment of DM121revealed that allele variation within D.officinale was attributed mainly to the variation of SSR repeat numbers,whereas allele variations among Dendrobium species were also caused by a single base indel and substitution in the microsatellite flanking region.
{"title":"Development and Transfer Analysis of SSR in Dendrobium","authors":"Qiu Daoshou, Xi-Long Zheng, Cai Shike, Jin-Rong Zheng, Lu Huanming, Zhang Lei, Deng Rui-yun, Wu Li, Liu Xiaojin","doi":"10.3724/SP.J.1142.2013.50500","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.50500","url":null,"abstract":"To date,only 100SSR markers in Dendrobiumhave been developed,which are far from sufficient for research applications.To develop molecular markers,we mined SSR of Dendrobium from public nucleotide data through bioinformation methods.Some 1343Uni-DNA sequences were assembled from the 3599DNA sequences of Dendrobiumfrom GenBank.By scanning the Uni-DNA sequences,283SSRs were distributed in 205Uni-DNA sequences,with an average frequency of 1SSR per 2815bp.Sequence alignment indicated that 86of the 205SSR-DNA sequences had already been used to design primers.In this study,76primer pairs were designed from the remaining 119sequences for transferability analysis among 32 Dendrobium species.Results showed that 47primer pairs were amplified effectively with transfer rates ranging from 51.1%to 95.7%(average75.9%).Of which,46primer pairs were able to detect polymorphism among the Dendrobium species with 2-8alleles(average 4.0alleles).Ten pairs of polymorphic primers were selected to detect polymorphism in 60accessions of D.officinale,and 2-5alleles(average 3.4alleles)were found per SSR locus.Based on the SSR amplification pattern,the 60accessions of D.officinale were clustered into five clusters,and phenotypes were closer within clusters than between clusters.The sequencing of the amplified fragment of DM121revealed that allele variation within D.officinale was attributed mainly to the variation of SSR repeat numbers,whereas allele variations among Dendrobium species were also caused by a single base indel and substitution in the microsatellite flanking region.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"500"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69789268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1142.2013.60616
Mei-Juan Zhang, W. Sha
The effects of different disinfectants on explants of Racomitrium japonicum were studied here. Suitable inoculation media were screened tentatively,and the effects of sucrose concentrations on growth of R. japonicum gametophytes were investigated. The results showed that a concentration of 0. 02% HgCl 2 for 45- 60 seconds was optimal for initiation cultures from fragments of gametophytes pre-immersed in 2% detergent for a period of time. The suitable inoculation medium for sterile explants was organic culture medium MS. Protonemal colonies and young gametophytes then grew well when 3% sucrose was added.
{"title":"Research on Tissue Culture Technology of Racomitrium japonicum","authors":"Mei-Juan Zhang, W. Sha","doi":"10.3724/SP.J.1142.2013.60616","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.60616","url":null,"abstract":"The effects of different disinfectants on explants of Racomitrium japonicum were studied here. Suitable inoculation media were screened tentatively,and the effects of sucrose concentrations on growth of R. japonicum gametophytes were investigated. The results showed that a concentration of 0. 02% HgCl 2 for 45- 60 seconds was optimal for initiation cultures from fragments of gametophytes pre-immersed in 2% detergent for a period of time. The suitable inoculation medium for sterile explants was organic culture medium MS. Protonemal colonies and young gametophytes then grew well when 3% sucrose was added.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"616"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69789938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.3724/SP.J.1142.2013.20171
Juan Wu, Lu-Yang Zou, Qun Zeng, Hong Xu
Prokaryotic cytoskeletal protein FtsZ,a microtubule homolog,assembles into a compact circular structure at the mid-cell and plays an important role in cytokinesis.To explore the function of FtsZ in Spirulina platensis morphogenesis,we cloned the ftsZ gene from S.platensis and constructed its fusion tag of GFP expression plasmid pGFP-FtsZ.The recombined expression vector was transformed to Escherichia coli BL21.Western blot analysis showed that the GFP-FtsZ fusion gene was successfully expressed in the transformant.The transformed bacteria that expressed the GFP-FtsZ protein changed from normal short-rod shapes and formed long filaments.The length of the filamentation cells was proportional to the expression amount of FtsZ in cells.Regular-dot distribution of the GFP-FtsZ fusion protein in transformed bacteria was observed by fluorescent light microscopy.The data demonstrated that FtsZ was a highly conserved functional protein.The FtsZ of S.platensis assembled the complete cytokinesis apparatus and formed a Z-ring structure at the future division site to regulate cell division in E.coli.The overexpression of FtsZ blocked the normal cell cycle and led to cell filamentation.
{"title":"Expression and Localization of FtsZ from Spirulina platensis in Escherichia coli","authors":"Juan Wu, Lu-Yang Zou, Qun Zeng, Hong Xu","doi":"10.3724/SP.J.1142.2013.20171","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.20171","url":null,"abstract":"Prokaryotic cytoskeletal protein FtsZ,a microtubule homolog,assembles into a compact circular structure at the mid-cell and plays an important role in cytokinesis.To explore the function of FtsZ in Spirulina platensis morphogenesis,we cloned the ftsZ gene from S.platensis and constructed its fusion tag of GFP expression plasmid pGFP-FtsZ.The recombined expression vector was transformed to Escherichia coli BL21.Western blot analysis showed that the GFP-FtsZ fusion gene was successfully expressed in the transformant.The transformed bacteria that expressed the GFP-FtsZ protein changed from normal short-rod shapes and formed long filaments.The length of the filamentation cells was proportional to the expression amount of FtsZ in cells.Regular-dot distribution of the GFP-FtsZ fusion protein in transformed bacteria was observed by fluorescent light microscopy.The data demonstrated that FtsZ was a highly conserved functional protein.The FtsZ of S.platensis assembled the complete cytokinesis apparatus and formed a Z-ring structure at the future division site to regulate cell division in E.coli.The overexpression of FtsZ blocked the normal cell cycle and led to cell filamentation.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"171"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69787730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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