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Bellemerea alpina—A New Lichen Species Record from China 标题中国地衣新种记录
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.40333
Gulbostan·ISMAY Abdulla·ABBAS
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引用次数: 2
Slr1122 Affects Activity of Hybrid Sensory Kinase and Regulates the Biosynthesis of Carotenoids inSynechocystissp.PCC 6803 Slr1122影响胞囊植物杂交感觉激酶活性及调节类胡萝卜素的生物合成。PCC 6803
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.30248
Xiang Li, Liang-Liang Dong, Lun Zhang, K. Zhao, Ming Zhou
{"title":"Slr1122 Affects Activity of Hybrid Sensory Kinase and Regulates the Biosynthesis of Carotenoids inSynechocystissp.PCC 6803","authors":"Xiang Li, Liang-Liang Dong, Lun Zhang, K. Zhao, Ming Zhou","doi":"10.3724/SP.J.1142.2013.30248","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.30248","url":null,"abstract":"","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"248"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69788511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cloning and Expression Analysis of Cinnamyl Alcohol Dehydrogenase (SmCAD) Gene inSalvia miltiorrhizaBunge 丹参肉桂醇脱氢酶(SmCAD)基因的克隆与表达分析
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.30261
Q. Ge, Jingge Kuang, Yucui Wu, Y. Zhang, Ya-Ping Xiao, Zhe-zhi Wang
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引用次数: 0
Molecular Cloning and Characterization of Isoflavone Synthase Gene from Pueraria lobata 葛根异黄酮合成酶基因的克隆与特性研究
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.40398
Junbo Gou, Changfu Li, Fangfang Chen, Zhaobo Li, Jia Li
Isoflavones are the main active component of kudzu(Pueraria lobata).The first committed step in the production of isoflavones is performed by a cytochrome P450 enzyme,IFS.While IFS genes have been isolated from kudzu,no biological functions have been identified to date.We cloned an IFS gene via RT-PCR,named PlIFS,using kudzu collected from Langxi(Anhui,China)as plant material.The PlIFS contained an open reading frame(ORF)encoding 521 amino acid residues.The amplified PlIFS gene was cloned into pESC-TRP vector,with yeast GAL1 divergent promoters as the control.The constructed recombinant plasmid,named pESC-TRP-PlIFS,was transformed into Saccharomyces cerevisiae strain WAT11 according to the LiAc/ssDNA/PEG method.The PlIFS expressed in yeast was able to catalyze daidzein formation from liquiritigenin,indicating that it had IFS activity.Gene expression pattern analysis revealed that PlIFS was expressed in roots at the highest level,which was consistent with isoflavones accumulation in kudzu plantlets.
异黄酮是葛根的主要活性成分。异黄酮生产的第一步是由细胞色素P450酶IFS完成的。虽然已经从葛中分离出IFS基因,但迄今为止还没有发现其生物学功能。本研究以安徽朗溪葛根为材料,通过RT-PCR技术克隆了IFS基因,命名为PlIFS。该PlIFS包含一个开放阅读框(ORF),编码521个氨基酸残基。将扩增的PlIFS基因以酵母GAL1发散启动子为对照,克隆到pESC-TRP载体中。将构建的重组质粒命名为pESC-TRP-PlIFS,按照LiAc/ssDNA/PEG法将重组质粒转化为酿酒酵母WAT11。在酵母菌中表达的PlIFS能够催化利尿素生成大豆苷元,表明其具有IFS活性。基因表达谱分析显示,PlIFS在根中表达量最高,这与葛根中异黄酮的积累一致。
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引用次数: 2
Techniques and Methods for the Practical Use of Xylem Pressure Probes 木质部压力探头实际应用的技术和方法
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.60603
X. Bai, Jian-jun Zhu
Xylem pressure probes are the only technique used currently to measure plant xylem pressure directly. This technique includes devices for accurate positioning,pressure probe systems and signal acquisition. Negative pressure of the xylem vessels is transmitted to the pressure sensor by conduction medium when the capillary probe punctures the xylem vessels, with the pressure then detected and transmitted to the data acquisition system. In this paper, the use and precautions of xylem pressure probes are described in detail,including the preparation of glass capillary probes,degassed water,pressure calibration,installation,and measurement of pressure probes.
木质部压力探针是目前唯一用于直接测量植物木质部压力的技术。该技术包括用于精确定位的设备,压力探头系统和信号采集。毛细管探针刺入木质部导管时,将木质部导管的负压通过传导介质传递给压力传感器,检测压力并将压力传输给数据采集系统。本文详细介绍了木质部压力探头的使用和注意事项,包括玻璃毛细管探头的制备、脱气水、压力校准、压力探头的安装和测量。
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引用次数: 0
Effects ofMicrolepia strigosaunder Drought Stress on Physiological Change Laws 干旱胁迫下小斜视虫生理变化规律的影响
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.60576
Hao Sun, Qian Wang, Yang Guan, Baowen Liu
Research on Microlepia strigosa under natural drought stress treatment exhibited changes in physical properties. Stress increased relative conductivityꎬ POD activityꎬ soluble sugar and proline content. Soluble protein and MDA content high ̄low ̄highꎻ SOD activityꎬ chlorophyll contentꎬ total respiratory rateꎬ net photosynthetic rate Intercellular CO2 concentration and stomatal conductance is on the declineꎻ The CAT activity and Transpiration rate is raise ̄lower. The membrane system did not exhibit serious damage. The osmotic regulation system had good resistance to dehydration. The protective enzyme system played a role against free radicalsꎻ respiration and photosynthesis decreased significantlyꎬ but mainly for autonomic regulation of the plant. Results demonstrated that M􀆰 strigosa adapted to stressꎬ and showed a strong ability of recovery after drought and exhibited certain endurance ability.
研究表明,自然干旱胁迫处理下的糙皮小蠹物理性状发生了变化。胁迫增加了相对电导率ꎬPOD活性ꎬ可溶性糖和脯氨酸含量。可溶性蛋白和MDA含量高/低/高ꎻSOD活性ꎬ叶绿素含量ꎬ总呼吸速率ꎬ净光合速率细胞间CO2浓度和气孔导度呈下降趋势ꎻCAT活性和蒸腾速率呈上升/下降趋势。膜系统未出现严重损伤。渗透调节系统具有良好的抗脱水性能。保护酶系统对自由基起作用ꎻ呼吸和光合作用显著降低ꎬ,但主要是植物的自主调节作用。结果表明:􀆰糙毛藻适应逆境ꎬ,表现出较强的旱后恢复能力和一定的耐旱能力。
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引用次数: 1
Leaf abaxial epidermal features of Chrysanthemum wild plants from Anhui. 安徽野菊花叶背面表皮特征。
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.10099
Zhen Lan-Ping, Yang Jun, Yu Nian-jun
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引用次数: 0
Chromosomal Study on Chinese Bupleurum L.(Apiaceae) 中华柴胡(蜂科)染色体研究
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.10011
Qian-Long Liang, Chang-Bao Wang, Xiang-Guang Ma, Cai Zhao, Xing-Jin He
Chromosome numbers of 24 populations from 13 species and 5 varieties,as well as karyotype data from 5 species and 1 variety of Chinese Bupleurum L.were reported.All were diploids,except two populations of B.tianschanicum,one of B.commelynoideum and one of B.marginatum were polyploids.(1) B.longicaule var.giraldii and B.petiolulatum var.tenerum may be two independent species;it is more appropriate for B.boissieuanum to be treated as a variety of B.longiradiatum,whereas treating B.commelynoideum var.flaviflorum as a variety of B.commelynoideum may not be appropriate;(2)B.commelynoideum and B.chinense are complex groups,which require further study;(3) The ancestral basic chromosome number of Bupleurum L.is x = 7,but this needs further evidence.
报道了柴胡属13种5变种24居群的染色体数目和5种1变种的核型资料。都是二倍体,除了两种群B.tianschanicum B.commelynoideum之一,多倍体植株B.marginatum之一。(1)B.longicaule var.giraldii和B.petiolulatum var.tenerum可能是两个独立的物种;更适合B.boissieuanum被视为各种B.longiradiatum,而治疗B.commelynoideum var.flaviflorum各种B.commelynoideum可能不合适;(2)B.commelynoideum B.chinense是复杂的组织,这需要进一步的研究;(3)柴胡祖先的基本染色体数为x = 7,但这需要进一步的证据。
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引用次数: 2
Study on Nutrition and Antioxidant Activities ofSterculia nobilisSmith Seeds 大栗种子营养及抗氧化活性的研究
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.20203
H. Ren, Jing Zhou, Yi-Wei Li, C. Wei, Yu-Ying Lu, Yan Lu, R. Luo
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引用次数: 3
Effect of Morphology and Physiology of Wetland Plants on Plateaus at Different Altitudes 不同海拔高原湿地植物形态生理的影响
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.40370
Juan Li, P. Lin, Yu Dong, Xu-Hu Guo
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引用次数: 2
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