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Study on the Anatomical Structures in Development of the Nurse Seed Grafted Union of Camellia oleifera 油茶种子嫁接接穗发育的解剖结构研究
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.30313
Zhijian Yang, Jinling Feng, Hui Chen
Conventional paraffin sectioning was adopted to conduct anatomical examination of the nurse seed grafted Camellia oleifera union during development.Results showed that at day 4 after grafting,the nurse seed grafting union became the isolation layer;at day 8,the callus in the root stock was observed;at day 16,the callus was found in the scion;at day 22,the root stock connected the scion;at day 29,the nurse seed grafting union formed the vascular cambium;at day 35,vascular bundles was formed,which indicated that the nurse seed grafted seedling survived.Before induction of callus in the nurse seed grafted C.oleifera union,the primary organization structure of the stock accelerated growth and development,and gradually became similar to the structure of the scion.The cambium and other parenchyma cells in the grafted union could all be induced to produce callus,but the cambium was the principal source.
采用常规石蜡切片法对育苗嫁接油茶愈合体发育过程进行解剖观察。结果表明,在嫁接后第4天,奶子籽嫁接接穗形成隔离层,第8天,砧木中出现愈伤组织,第16天,接穗中出现愈伤组织,第22天,砧木与接穗连接,第29天,奶子籽嫁接接穗形成维管形成层,第35天,维管束形成,说明奶子籽嫁接的幼苗成活。乳子嫁接油松愈伤组织诱导前,砧木初生组织结构加速生长发育,逐渐向接穗结构靠拢。嫁接结合中的形成层和其他薄壁细胞均可诱导产生愈伤组织,但形成层是愈伤组织的主要来源。
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引用次数: 4
Geographic Distribution Characteristics and Endangered Status of an Endemic Species of China,Paris undulatisH. Li et V. G. Soukup 中国特有种波波兰的地理分布特征及濒危状况。李等V. G. Soukup
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.40328
Hongxiang Yin, Feiyan Wen, Tiezhu Chen, Hao Zhang
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引用次数: 1
Determination of Puerarin,Daidzin and Daidzein in Roots and Leaves of Pueraria lobata(Wild) Ohwi from United States by RP-HPLC 反相高效液相色谱法测定美国野生葛根和叶中葛根素、大豆苷元和大豆苷元的含量
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.40391
Xuefang Yang, Wei Huang, Jianqing Ding
In this study,we measured three isoflavonoids in kudzu from eight populations in south-eastern United States.The concentrations of puerarin,daidzin and daidzein in the leaves were 0.0950 mg/g,0.698 mg/g and 0.110 mg/g,respectively.The concentration of daidzin and daidzein was significantly different among populations.During the growing season,three isoflavonoids in the leaves were higher in August than in July and September.The concentrations of the three isoflavonoids in the roots were 3.664 mg/g,1.0302 mg/g and 0.3689 mg/g,respectively.There were no significant differences in the three isoflavonoids among populations.Furthermore,the concentrations of the three isoflavonoids in kudzu from US populations were higher than those of Chinese populations,as reported in previous research.This study aimed to provide a theoretic and scientific basis for making full use of kudzu leaves,determining appropriate harvest times and the development and utilization of the puerarin leaves resource.
在这项研究中,我们测量了来自美国东南部八个种群的葛根中的三种异黄酮。叶中葛根素、大豆苷元和大豆苷元的含量分别为0.0950 mg/g、0.698 mg/g和0.110 mg/g。大豆苷元和大豆苷元的浓度在不同种群间存在显著差异。在生长季节,8月份叶片中3种异黄酮含量均高于7月和9月。根中3种异黄酮的含量分别为3.664 mg/g、1.0302 mg/g和0.3689 mg/g。3种异黄酮在不同种群间无显著差异。此外,美国人群葛根中三种异黄酮的含量高于中国人群,这与以往的研究结果一致。本研究旨在为充分利用葛根叶、确定适宜采收期和开发利用葛根叶资源提供理论和科学依据。
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引用次数: 0
Pollen Morphology ofMelodorum fruticosumLour. 紫苏的花粉形态。
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.50439
Gui-Fang Yang, Yong Liu, Fengxia Xu
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引用次数: 0
Flora Study of Riparian Plants on the Mountain River Banks of the Jiulongjiang River Headstream 九龙江源区山间河岸植物区系研究
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.50467
Wei Deng, R. Sun, Xiu-Ming Li, D. Lu, Quan Yang, Kewei Lu
Based on field investigation,sample collection,and comprehensive analysis of the riparian vegetation in mountain rivers,the riparian flora characteristics were investigated.The results showed that 574vascular plants belonging to 390genera and 145families in the mountain rivers of the Jiulongjiang River headstream were identified,among which pteridophyte had 29families,54genera and 80species;gymnosperm had 6families,8genera and 10species;and,angiosperm had110families,328 genera and 484 species.Through comprehensive analysis several typical characteristics of the mountain river riparian vegetation(taking reaches of the Jiulongjiang River headstream as an example)were found as follows:(1)a rich floral composition,with the dominant families and genera highly prominent;monotypic,oligotypic and oligotaxic genera,and oligotypic and oligotaxic families formed a large proportion;(2)flora of ancient origin;rare and relict plants were abundant;and,(3)complicated geographical elements and flora connected extensively with most parts of the world fully reflected natural and human disturbance such as regional climate,topography and human activities.Results indicated that the riparian zone had an extremely important function in conserving regional biodiversity.
通过野外调查、样品采集和综合分析,对山区河流河岸植物区系特征进行了研究。结果表明:在九龙江源头山区河流中共鉴定出维管植物390属145科574种,其中蕨类植物29科54属80种,裸子植物6科8属10种,被子植物110科328属484种。通过综合分析,以九龙江源区为例,发现山地河流河岸植被具有以下几个典型特征:(1)植物组成丰富,优势科和优势属极为突出,单、寡、寡分类属较多,寡、寡分类科占较大比例;(2)植物区系源远流长,珍稀、遗存植物较多;(3)地理要素和区系复杂与世界大部分地区广泛相连,充分反映了区域气候、地形、人类活动等自然和人为干扰。结果表明,河岸带在保护区域生物多样性方面具有极其重要的作用。
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引用次数: 3
New Observations on the Morphology and Structure ofMarchantia polymorphaGametophores in Sexual Reproduction Adaption 马钱兰多态配子体形态结构在有性生殖适应中的新观察
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.60555
Jian-Guo Cao, Quan-Xi Wang, Hong-Mei Zou, Xiling Dai, Tong Cao
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引用次数: 4
Effects of Cadmium Stress on Seedlings Growth and Active Ingredients in Salvia miltiorrhiza 镉胁迫对丹参幼苗生长及有效成分的影响
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.60583
X. Zhang, Kun-Wei Li, Kang-Jian Chen, Jian Liang, L. Cui
Effects of cadmium stress on seedlings growth and active ingredients in Salvia miltiorrhiza were investigated. Results showed that cadmium stress inhibited seedlings growth and increased Cd2+accumulation in both soil and seedlings. Moreover,both soluble protein content and membrane lipid peroxidation degree increased,while photosynthetic pigments content decreased under stress conditions. Compared with the control, in stressed S. miltiorrhiza leaves,the accumulation of six phenolic acid compounds showed different variant characteristics. Both caffeic acid and rosmarinic acid accumulation decreased significantly( p0. 05),while the content of protocatechuic acid increased( p0. 05),and the levels of tanshinol,protocatechuic aldehyde and salvianolic acid B decreased( p 0. 05). However, the accumulations of the phenolic acids components and four tanshinone ingredients all decreased in stressed roots, and the variances of rosmarinic acid, dihydrotanshinone, tanshinone Ⅰ and cryptotanshinone were significant( p 0. 05). Moreover,in the leaves of the stressed plant,the activities of PAL and TAT decreased significantly,but C4H and 4CL content increased significantly. These results showed that cadmium stress could reduce the production and quality of S. miltiorrhiza.
研究了镉胁迫对丹参幼苗生长及有效成分的影响。结果表明,镉胁迫抑制了幼苗生长,增加了土壤和幼苗中Cd2+的积累。胁迫条件下可溶性蛋白含量和膜脂过氧化程度均升高,光合色素含量降低。与对照相比,胁迫下丹参叶片中6种酚酸类化合物的积累表现出不同的变异特征。咖啡酸和迷迭香酸积累均显著减少(p < 0.05)。原儿茶酸含量显著升高(p < 0.05)。丹参醇、原儿茶醛和丹酚酸B水平降低(p < 0.05)。05). 而酚酸类成分和丹参酮4种成分的积累量在胁迫下均呈下降趋势,迷迭香酸、二氢丹参酮、丹参酮Ⅰ和隐丹参酮的积累量差异显著(p < 0.05)。05). 此外,胁迫植株叶片中PAL和TAT活性显著降低,C4H和4CL含量显著升高。结果表明,镉胁迫会降低丹参的产量和品质。
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引用次数: 8
Feasibility Study on the Identification of GenusMacromitriumBased on Four Chloroplast Genes 基于4个叶绿体基因鉴定巨噬菌属的可行性研究
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.10023
Dandan Li, Shuiliang Guo, Jing Yu, Sha Li, T. Cao
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引用次数: 3
Measuring Pattern Diversity of Hubei Province Vegetation Based on Affinity Analysis 基于亲和分析的湖北省植被格局多样性测度
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.20124
Lamei Cheng, J. Ge, Zhao-Jun Wu, Zi-Ye Wang, Jianfeng Li
Taking formation as an index,the vegetation pattern diversity of Hubei Province was measured by affinity analysis.Results showed that mosaic diversity(m) of the Hubei Province vegetation landscape was 4.071,the landscape was complicated and there was no obvious ecological gradient.According to mean affinities(MA),the Hubei Province vegetation pattern was divided into three categories: the first central point category included vegetation from Wuhan City,Xiangyang City,Huanggang City and Suizhou City,which were typical,common,and played a key role in coupling patches in the whole vegetation pattern;the second outer point category included vegetation from Hefeng County,Lichuan City and Xianfeng County,which were rare,native and had important significance in composing the specificity and diversity of the whole landscape;the third middle point category included vegetation from Huangshi City,Jingzhou City,Jingmen City,and Yichang City(totally 14 administrative division units),which showed universality and rarity,were moderate,occupied more habitat space,and constituted the foundation of the whole vegetation pattern.Sorting mean similarity(MS) from central points to outer points showed that the similarity of adjacent regions was high.Northwest,Southeast and the Jianghan plain of Hubei formed the universality of the whole vegetation pattern;however Southwest Hubei constituted the specificity of the vegetation pattern.Large differences existed in the vegetation patterns between east and west Hubei Province,and the vegetation patterns were complicated.
以形成为指标,采用亲和分析方法对湖北省植被格局多样性进行了测度。结果表明:湖北省植被景观的马赛克多样性(m)为4.071,景观较为复杂,不存在明显的生态梯度;根据平均亲和度(MA),将湖北省植被格局划分为3类:第一类中心点包括武汉市、襄阳、黄冈市和随州市的植被,它们是典型的、常见的,在整个植被格局中起着耦合斑块的关键作用;第二类中心点包括鹤峰县、利川市和咸丰县的植被,它们是稀有的、原生的,对构成整个景观的特异性和多样性具有重要意义黄石市、荆州市、荆门市和宜昌市(共14个行政区划单位)具有普遍性和稀有性,分布适中,占据较多的生境空间,构成了整个植被格局的基础。从中心点到外点的平均相似度(MS)排序表明相邻区域的相似度较高。湖北西北部、东南部和江汉平原构成了整个植被格局的普遍性,而湖北西南部构成了植被格局的专一性。鄂东、鄂西植被格局差异较大,植被格局较为复杂。
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引用次数: 0
Distribution of Pistillate Flowers and Disciplines of Fruit-set Success in Rubber Tree (Hevea brasiliensisMuell. Arg.) 橡胶树雌蕊花分布与坐果成功规律。参数)。
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.30242
Yuan-Yuan Zhang, Guo-hua Li, Qing-yi Long, Li-gang He, X. Kang
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引用次数: 0
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Plant Science Journal
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