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Determination of Bactericidal Potential of Green Based Silver and Zinc Nanoparticles against Bacterial Canker of Tomato 绿基银、锌纳米粒子对番茄细菌性溃疡病杀菌电位的测定
IF 1.3 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-28 DOI: 10.33804/pp.006.03.4318
M. Atiq, Maryam Ashraf, N. Rajput, S. T. Sahi, A. Akram, M. Usman, S. Iqbal, Ahmad Nawaz, A. M. Arif, Abuzar Hasnain
Bacterial canker of tomato is the most damaging one and is responsible for causing 70% yield losses each year. Green synthesis of nanoparticles has ushered in a new research field known as green nanotechnology and has emerged as vital tool for the management of plant diseases. In comparison to other conventional techniques, green synthesis of various nanoparticles was found to be eco-friendly, low-cost and profitable resulting in more stable synthesized materials. In present research, zinc and silver nanoparticles were synthesized and their antibacterial potential was evaluated alone and in combination against Clavibacter michiganensis subsp. michiganensis under lab and field conditions. In lab experiment, combination of Zinc and Silver NPs proved to be the most effective showing maximum inhibition zone (27.449 mm) followed by Zinc (19.50 mm), and Silver (16.21 mm). Similarly, in the field experiment combination of Ag+Zn NPs   proved effective with minimum disease incidence (6.10%) followed by Zinc (10.15%) and Silver NPs (22.40%) as compared to the control.
番茄细菌性溃疡病是危害最大的一种,每年造成70%的产量损失。纳米颗粒的绿色合成开创了一个新的研究领域——绿色纳米技术,并已成为植物病害管理的重要工具。与其他传统技术相比,各种纳米颗粒的绿色合成被认为是环保、低成本和有利可图的,从而使合成材料更加稳定。本研究合成了锌纳米粒子和银纳米粒子,并对其单独和联合抑菌效果进行了评价。实验室和田间条件下的密歇根。在实验室实验中,锌和银的NPs联合使用效果最好,抑制范围最大(27.449 mm),其次是锌(19.50 mm)和银(16.21 mm)。田间试验中,与对照相比,银+锌NPs组合有效,发病率最低(6.10%),其次是锌(10.15%)和银NPs(22.40%)。
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引用次数: 3
Chemical Profile and Antifungal Activity of Leaf Extract of Tabernaemontana divaricata against Macrophomina phaseolina 竹叶提取物对菜花大蠹的化学特征及抑菌活性研究
IF 1.3 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-28 DOI: 10.33804/pp.006.03.4332
A. Javaid, Iqra Khan
Macrophomina phaseolina is a highly problematic fungus that causes diseases in hundreds of plant species. In order to search for an alternative to chemical fungicides for the control of this devastating pathogen, leaf extract of Tabernaemontana divaricata was assessed for the control of M. phaseolina. Five concentrations of leaf extract in methanol viz. 1, 2, 3, 4 and 5% (w/v), were checked against M. phaseolina. All these concentrations significantly suppressed the fungal growth resulting in 34–74% decreased biomass of M. phaseolina over control. GC-MS analysis of this extract showed the presence of 54 compounds. Squalene was the predominant compound with 24.11% peak area followed by vitamin E (8.96%). Other important compounds were hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (6.21%), thiophene, tetrahydro-2-methyl- (5.68%), cyclopentanol (5.61%), neophytadiene (6.25%), 1-tert-butoxypropan-2-yl 2-methylbutanoate (6.03%), ibogamine-18-carboxylic acid, 12-methoxy-, methyl ester (2.95%), phytol (2.26%), and n-hexadecanoic acid (2.20%). Some of these major compounds might be responsible for antifungal property of methanolic leaf extract of T. divaricata against M. phaseolina.
phaseolina是一种非常有问题的真菌,它会导致数百种植物的疾病。为了寻找化学杀菌剂的替代品来防治这一毁灭性的病原菌,本研究对大黄叶提取物的防治效果进行了评价。5种浓度的甲醇叶提取物分别为1、2、3、4和5% (w/v),对菜叶分枝杆菌进行了检测。这些浓度均显著抑制了菜绿支原体的生长,使菜绿支原体生物量较对照减少34 ~ 74%。GC-MS分析表明该提取物含有54种化合物。角鲨烯为优势化合物,峰面积为24.11%,其次为维生素E,峰面积为8.96%。其他重要的化合物有:十六烷酸、2-羟基-1-(羟甲基)乙酯(6.21%)、噻吩、四氢-2-甲基(5.68%)、环戊醇(5.61%)、新植物二烯(6.25%)、1-叔丁基丙烷-2-基2-甲基丁酸酯(6.03%)、伊博gamine-18-羧酸、12-甲氧基-甲酯(2.95%)、叶绿醇(2.26%)和正十六烷酸(2.20%)。其中一些主要化合物可能是导致金针叶甲醇提取物对菜绿霉具有抗真菌作用的原因。
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引用次数: 1
Management of Fall Army Worm of Maize, Spodoptera frugiperda, with Green Synthesis Silver Nanoparticles 绿色合成纳米银对玉米秋虫的治理
IF 1.3 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-28 DOI: 10.33804/pp.006.03.4294
Ahmad Hassan Tahir, M. Tariq, Muhammad Shehzad
The fall armyworm (FAW), Spodoptera frugiperda is a polyphagous and economically important insect pest originated from tropical and sub-tropical parts of America. Being a strong flier, climatic adaptation and large host range makes it a serious threat to food security. It is responsible for high economic losses in many cash crops, vegetables and cereals. The overreliance and overuse of pesticides for the management of FAW posed many problems including insecticide resistance, resurgence of insect pests, biotype development and environmental hazards. To combat these problems, the most important alternative is the use of green synthesis nanoparticles and biocontrol agents which have been proven as eco-friendly technology for pest control. The current study was, therefore, planned to evaluate the toxicity of different plant based synthetic nanoparticles and biocontrol agent (Trichogramma spp.) against fall armyworm. Silver nanoparticles of neem resulted in the highest (83%) and the lowest (40%) mortality of 2nd instar larvae of FAW. However, the highest mortality due to silver nanoparticles of tobacco, onion, mint, ginger and datura was observed as 86%, 63%, 76%, 63% and 73% while the lowest mortality was 30%, 33%, 30%, 23% and 16% respectively. In view of the current findings nanoparticles of datura and neem could be recommended as potential bio-based chemicals for the control of S. frugiperda.
秋粘虫(Spodoptera frugiperda)是一种多食性害虫,原产于美洲热带和亚热带地区,具有重要的经济价值。由于其飞行能力强,气候适应性强,寄主范围广,对粮食安全构成严重威胁。它对许多经济作物、蔬菜和谷物造成了巨大的经济损失。由于对农药的过度依赖和过度使用,造成了农药抗药性、害虫死灰复燃、生物型发育和环境危害等诸多问题。为了解决这些问题,最重要的替代方案是使用绿色合成纳米颗粒和生物防治剂,这些已被证明是防治害虫的环保技术。因此,本研究计划评估不同植物合成纳米颗粒和生物防治剂(赤眼蜂spp.)对秋粘虫的毒性。印楝银纳米颗粒对2龄幼虫的死亡率最高(83%),最低(40%)。烟草、洋葱、薄荷、生姜和曼陀罗对纳米银颗粒的最高致死率分别为86%、63%、76%、63%和73%,最低致死率分别为30%、33%、30%、23%和16%。鉴于目前的研究结果,曼陀罗纳米颗粒和楝树纳米颗粒可以作为潜在的生物基化学药剂用于防治果蚜。
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引用次数: 0
Assessment of Toxicity of Parthenium hysterophorus L. Extract against Larvae of Trogoderma granarium 宫草Parthenium L.提取物对谷物Trogoderma granarium幼虫的毒性评价
IF 1.3 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-28 DOI: 10.33804/pp.006.03.4350
Shahbaz Ahmad, R. Zafar, Iqra Khan, A. Javaid, A. Intisar
The leaves of parthenium (Parthenium hysterophorus L.) were extracted in ethanol and toxicity of the extract was examined on khapra beetle (Trogoderma granarium Everts) larvae. Four concentrations (2, 4, 6 and 8 µL mL-1 acetone) were applied directly on the larvae in Petri dishes. Toxicity was recorded after 12, 24 and 36 h. All the concentrations had considerable effect on the mortality of larvae. At the highest concentration (8 µL mL-1), the mortality observed was 57%, 72% and 78% after 12, 24 and 36 h, respectively. The minimum mortality was observed to be 43% at the concentration of 2 µL mL-1 acetone after 12 h. GC-MS analysis of the parthenium leaves showed 7 compounds in the extract including phytol (38.68%), β-cubebene (11.98%), hexadecanoic acid, methyl ester (11.22%), caryophyllene (10.37%), 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z,Z)- (9.98%), methyl stearate (9.01%), and hexadecanoic acid, ethyl ester (8.74%). The study concluded that an extract of 8 µL mL-1 acetone concentration is highly effective against larvae of khapra beetles that can cause 78% mortality after 36 h of direct exposure to the larvae.
采用乙醇提取帕特嫩草(parthenium hysterophorus L.)的叶片,并对其幼虫进行毒性试验。4种浓度(2、4、6和8µL mL-1丙酮)直接作用于培养皿中的幼虫。12、24、36 h后分别记录毒力,各浓度对幼虫的死亡率均有显著影响。在最高浓度(8µL mL-1)下,12、24和36 h后的死亡率分别为57%、72%和78%。在2µL mL-1丙酮浓度下,12 h后的最低死亡率为43%。GC-MS分析显示,提取物中含有叶绿醇(38.68%)、β-三苯(11.98%)、十六烷酸甲酯(11.22%)、石竹烯(10.37%)、9,12,15-十八烷酸乙酯(Z,Z,Z)-(9.98%)、硬脂酸甲酯(9.01%)和十六烷酸乙酯(8.74%)等7种化合物。研究结果表明,8µL mL-1丙酮浓度的提取物对卡布拉甲虫幼虫非常有效,直接接触36 h后死亡率可达78%。
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引用次数: 1
Management of Chili Root Rot Pathogen, Rhizoctonia solani, with Potential Rhizobacteria 辣椒根腐病病原菌茄枯丝核菌的防治
IF 1.3 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-28 DOI: 10.33804/pp.006.03.4378
A. Mateen, M. A. Mehmood, M. Ashfaq, Abubakar Saddique, R. Binyamin, M. M. Ahmed, Hafiz Hamza Naeem
Chili (Capsicum annuum L.) belongs to the solanaceae family. It is a major vegetable crop grown on a wide scale in Asia. Nearly 20% of Pakistan's total vegetable producing land is utilized for cultivating chili. Various plant pathogens including viruses, fungi, bacteria and nematodes are responsible for causing diseases in chili crop. Among fungal diseases, root rot is an important fungal disease which is caused by Rhizoctonia solani. In the present study, a survey of different localities of Multan district were conducted and plants with prominent root rot symptoms were collected and subjected to characterization. Moreover, the disease incidence and prevalence was calculated in surveyed localities. Highest disease incidence i.e. 20.77% was evident in samples collected from Tatay Pur and the lowest disease incidence (7.59%) was observed in samples of Suraj Miani. Morphological identification of root rot samples was carried out based on growth, shape, color, and hyphae structure and confirmed the association of R. solani. Koch’s postulates were confirmed after pathogenicity test which was accomplished by stem application method. About 10 rhizobacteria were isolated from rhizospheric soils of healthy plants and dual culture technique was used to manage the R. solani mycelial growth under in vitro conditions. Our results revealed that rhizobacteria 7 (RB7) exhibited the highest inhibition percentage 54.81% while the lowest inhibition percentage i.e. 40% was evident using rhizobacteria 4 (RB4) compared to control. This study helped us to provide the alternatives to the chemical fungicides that could be explored under field conditions against root rot disease as they are ecofriendly and has less impact to the human life.
辣椒(Capsicum annuum L.)属于茄科。它是亚洲广泛种植的一种主要蔬菜作物。巴基斯坦近20%的蔬菜生产用地用于种植辣椒。各种植物病原体,包括病毒、真菌、细菌和线虫,都是辣椒作物致病的原因。在真菌病害中,根腐病是由枯丝核菌引起的一种重要的真菌病害。本研究对木尔坦地区不同地点进行了调查,收集了根腐病症状突出的植物并进行了鉴定。此外,还计算了调查地区的发病率和患病率。塔泰浦尔的发病率最高,为20.77%,苏拉米亚尼的发病率最低,为7.59%。根据根腐病样品的生长、形状、颜色和菌丝结构进行形态鉴定,证实了与茄茄霉的关联。通过茎施法进行致病性试验,证实了科赫的假设。从健康植物根际土壤中分离到10种左右的根瘤菌,采用双重培养技术在体外条件下对茄茄菌菌丝生长进行了控制。结果表明,7号菌(RB7)的抑菌率最高,为54.81%;4号菌(RB4)的抑菌率最低,为40%。该研究有助于我们在田间条件下探索化学杀菌剂防治根腐病的替代方案,因为它们对生态友好,对人类生活的影响较小。
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引用次数: 1
First Report of an Alien Whitefly Predatory Beetle, Delphastus pallidus (LeConte) (Insecta: Coleoptera: Coccinellidae) from South Punjab, Pakistan 巴基斯坦旁遮普南部外来白蝇掠食性甲虫Delphastus pallidus (LeConte)(昆虫纲:鞘翅目:瓢虫科)首次报告
IF 1.3 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-28 DOI: 10.33804/pp.006.03.4404
S. Hussain, Wali Muhammad
Delphastus pallidus (LeConte) is an important predatory beetle reported for effective control of whiteflies including Bemisia tabaci, Aleurotrachelus trachoides, Paraleyrodes bondari and Dialeurodes citrifolii. Many other members of genus Delphastus are well known predators of multiple whitefly species such as Delphastus catalinae (Horn). The members of this genus have predatory potential against all the stages of whitefly including eggs, nymphs and adults. This genus was reported in earlier 1950s from Florida on citrus whitefly. The later studies have reported it from Beach and the Mims areas in 1951, Indian Rocks Lake Alfred areas in 1953. It was not reported against cotton whitefly from anywhere of Pakistan. The Pest Warning Quality Control of Pesticides, Punjab team Multan reported it from cotton fields. Samples were collected and got identified from the Department of Entomology, Muhammad Nawaz Sharif University of Agriculture, Multan. After morphological and taxonomic identification, it was identified as Whitefly Predatory Beetle, Delphastus pallidus (LeConte) (Insecta: Coleoptera: Coccinellidae). Extensive surveys during the 3rd week of August 2022 reported its presence in 15 tehsils of cotton zone, South Punjab, Pakistan.
褐飞虱(Delphastus pallidus, LeConte)是一种重要的掠食性甲虫,据报道可有效防治烟粉虱、白粉虱、白粉虱和黄粉虱。许多其他成员的Delphastus属是多种白蝇物种,如Delphastus catalinae(角)众所周知的捕食者。本属的成员对所有阶段的粉虱包括卵、若虫和成虫都有捕食潜力。该属于20世纪50年代初在佛罗里达州的柑橘粉虱上被报道。后来的研究报告是1951年在海滩和米姆斯地区,1953年在印第安岩石和阿尔弗雷德湖地区。在巴基斯坦各地均未见对棉粉虱的防治报道。旁遮普害虫预警质量控制小组木尔坦报告了棉花田的情况。样本从木尔坦穆罕默德纳瓦兹谢里夫农业大学昆虫学系收集并鉴定。经形态学和分类学鉴定,鉴定为白蝇掠食性甲虫,Delphastus pallidus (LeConte)(昆虫亚目:鞘翅目:瓢虫科)。2022年8月第三周进行的广泛调查显示,该病毒在巴基斯坦南旁遮普棉花区15个省存在。
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引用次数: 0
Boeremia Exigua Leaf Spot: A New Emerging Threat to Gossypium hirsutum L. in Pakistan 巴基斯坦棉属植物叶斑病的新威胁
IF 1.3 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-28 DOI: 10.33804/pp.006.03.4275
W. Anwar, S. Javed, Farman Ahmad, A. Akhter, H. Khan, M. S. Haider, R. Kalsoom
Cotton is grown all over the world including Asian countries. Severe foliar disease symptoms were observed on cotton in fields from different districts of cotton zone of the province of Punjab, Pakistan. Infected plant leaves were sampled from various areas and processed for the isolation of the causal organism. Fungal isolates were identified morphologically. The conidia of the fungus were observed oblong to ellipsoid, hyaline, and aseptate, but occasionally 1-septate with dimensions ranging from 2.7 to 7.8 × 1.6 to 3.5 μm. A total of two isolates were characterized further for genetic diversity. Both the isolates showed moderate reactions to the NaOH test for E metabolite. The pathogen was morphologically identified as Boeremia exigua. Further, the internal transcribed spacer region (ITS) and TUB gene of the fungal isolates were amplified and analyzed. The sequence of partial ITS and TUB showed 99% homology with the isolates of B. exigua. The pathogenicity test was performed on three months old cotton plants by using two isolates of B. exigua. After 15 days of inoculation, necrotic spots started developing on the leaves that were very similar to those observed in the field. The fungal pathogen was re-isolated from the leaves of all the inoculated plants, identified morphologically following Koch's postulates. This disease might be a potential threat to cotton production in Pakistan in the future. However, further studies are required to know the virulence, behavior, alternate hosts, and spreading nature of the pathogen.
棉花在世界各地都有种植,包括亚洲国家。在巴基斯坦旁遮普省棉花区不同地区的棉花上观察到严重的叶面病症状。从不同地区取样受感染的植物叶片,并处理以分离致病生物。真菌分离株进行形态鉴定。真菌的分生孢子呈长圆形至椭球状,透明,无孢子,偶尔有1-间隔,尺寸为2.7 ~ 7.8 × 1.6 ~ 3.5 μm。对2个分离株进行了进一步的遗传多样性鉴定。两株菌株对E代谢物的NaOH试验均有中等反应。病原菌形态鉴定为布尔米菌。进一步对真菌分离物的内部转录间隔区(ITS)和TUB基因进行扩增和分析。部分ITS和TUB序列与该菌株同源性达99%。用2株小夜蛾对3月龄棉花进行了致病性试验。接种15天后,叶片上开始出现坏死斑,与田间观察到的坏死斑非常相似。从所有接种植株的叶片中重新分离出真菌病原体,根据科赫的假设进行形态学鉴定。这种疾病可能是巴基斯坦未来棉花生产的潜在威胁。然而,需要进一步的研究来了解病原体的毒力、行为、交替宿主和传播性质。
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引用次数: 3
SENSITIVITY OF THE CAUSAL AGENT OF APPLE SCAB VENTURIA INAEQUALIS FUNGUS TO KRESOXIM-METHYL IN COMMERCIAL ORCHARDS OF BELARUS 白俄罗斯商业果园苹果褐斑菌病原菌对甲基克雷索辛的敏感性
IF 1.3 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-10-21 DOI: 10.47612/0135-3705-2022-46-103-110
V. S. Komardina, E. V. Vasekha, R. I. Pleskatsevich
In this article, the results of the researches carried out in 2019-2022, based on the monitoring of apple scab agent resistance to kresoxim-methyl in commercial orchards of the republic are presented. The influence of 3-time applications of monocomponent and combined fungicides from strobilurin group on sensitivity of V. inaequalis fungus to kresoxim-methyl and their biological efficacy in apple scab control have been assessed.
本文介绍了2019-2022年在我国商品果园进行的苹果赤霉病剂对甲基克雷索辛抗性监测的研究结果。研究了三次施用单组杀菌剂和双组杀菌剂对苹果赤霉病菌的敏感性及防治效果的影响。
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引用次数: 0
ABOUT MILLET CROPS PESTS (LITERATURE REVIEW) 关于谷子作物害虫(文献综述)
IF 1.3 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-10-21 DOI: 10.47612/0135-3705-2022-46-193-205
A. V. Bykovskaya, S. V. Boiko
The paper presents the analysis of foreign, Russian and national publications about pests of millet crops during their vegetation. Pests belonging to different families and orders dominate globally and damage millet crops during the whole period of their development: sprouts, vegetative (leaves and stalks) and generative organs. It’s established that corn borer (Ostrinia nubilalis Hbn.) is the most dangerous phytophage of common millet and African millet in Belarus. In 2021 the damage caused by corn borer to crops at the milk-dough stage was 87,5–90,0 % and 15,0–34,0 % respectively.
本文对国外、俄罗斯和国内有关谷子植物生长期间有害生物的文献进行了分析。害虫在全球范围内占主导地位,属于不同的科目,在谷子作物的整个发育阶段:芽、营养(叶和茎)和生殖器官。在白俄罗斯,玉米螟(Ostrinia nubilalis Hbn.)是普通谷子和非洲谷子最危险的植噬体。2021年,玉米螟对乳粉期作物的危害分别为87% ~ 90%和15% ~ 34%。
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引用次数: 0
EFFICIENCY OF LIFILINE, WS HERBICIDE IN NEAR TRUNK LANES OF THE GARDEN lifiline, ws除草剂在花园主干道附近的效果
IF 1.3 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-10-21 DOI: 10.47612/0135-3705-2022-46-43-48
R. V. Supranovich, E. Berlinchik, S. O. Moshtyl
The research on evaluating the biological efficiency of Lifeline, WS herbicide (glufosinate ammonium, 280 g/l), UPL Holdings Ltd, the Netherlands, was carried out in the field experiments in near trunk lanes of the apple garden. It’s established that Lifeline, WS with a three time application to fruit plants in a dose of 1,1 and 1,8 l/ha showed a high biological efficiency against perennial and annual weeds (100 %). By the time of harvesting near trunk lanes of the garden were free of weed plants, which was very important for harvesting. On the basis of the obtained data Lifeline, WS (glufosinate ammonium, 280 g/l) was included in the State List of Plant Protection Means and Fertilizers Approved for Use in the Republic of Belarus.
以荷兰UPL公司生产的WS除草剂(草铵膦,280 g/l)为研究材料,在苹果园主干道附近进行了田间试验。结果表明,以1,1和1,8 l/ha的剂量三次施用于果树,对多年生杂草和一年生杂草的生物防治效率均达到100%。在收获的时候,花园主干道附近没有杂草植物,这对收获非常重要。根据获得的数据生命线,将WS(草铵膦,280 g/l)列入白俄罗斯共和国批准使用的植物保护手段和肥料国家清单。
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引用次数: 0
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Plant protection science
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