Postharvest Biology and Technology最新文献_第3页

Fumigation with basil essential oil enhances the resistance of blueberry fruit to gray mold by modulating carbohydrate and phenylpropanoid metabolic pathways 罗勒精油熏蒸通过调节碳水化合物和苯丙类代谢途径增强蓝莓果实对灰霉病的抗性

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-11-26 DOI: 10.1016/j.postharvbio.2025.114096

Zhaoyuan Wang , Yajun Wang , Canying Li , Xian Ji , Ling Zhang , Wendan Qu , Xin Fang , Yonghong Ge

Botrytis cinerea, the causal agent of gray mold, is a major contributor to postharvest losses in blueberry fruit. This study was undertaken to assess the inhibitory impacts of basil essential oil (BEO) fumigation on B. cinerea both in vivo and in vitro, as well as to evaluate its influence on carbohydrate and phenylpropanoid metabolic pathways in blueberries. The results demonstrated that fumigation with 0.04 mL L⁻¹ BEO effectively suppressed the in vitro growth of B. cinerea mycelium and spore germination, compromised cell membrane integrity, and induced the leakage of intracellular proteins and nucleotides. In vivo experiments demonstrated that fumigation with 0.04 mL L⁻¹ BEO delayed the decline in soluble and reducing sugars levels by improving amylase, sucrose synthase-synthesis, and sucrose phosphate synthase activities and their corresponding gene expressions, while simultaneously suppressing the acid convertase, neutral convertase, sorbitol oxidase, sucrose synthase-cleavage, NADP-sorbitol dehydrogenase, and NAD-sorbitol dehydrogenase activities and their corresponding gene expressions. Additionally, BEO fumigation enhanced the enzymatic activities and up-regulated the gene expressions level of key enzymes associated with phenolic biosynthesis, including phenylalanine ammonia-lyase, 4-coumaroyl-CoA ligase, cinnamyl alcohol dehydrogenase. Additionally, BEO fumigation significantly enhanced the concentrations of endogenous phenylalanine, p-coumaric acid, caffeic acid, and sinapic acid, thereby promoting the accumulation of total phenolics and lignin in blueberries. Overall, BEO exerts direct inhibitory effects on the mycelial growth and spore germination of B. cinerea by disrupting cell membrane integrity. Furthermore, BEO fumigation enhances disease resistance in blueberry fruit against B. cinerea by activating key enzymatic activities and gene expressions involved in carbohydrate and phenylpropanoid metabolic pathways, leading to increased accumulation of phenolic compounds.

灰霉病的病原灰霉病菌灰霉病菌是蓝莓果实采后损失的主要原因。本研究旨在研究罗勒精油（BEO）熏蒸对蓝莓绿杆菌的体内和体外抑制作用，以及对蓝莓碳水化合物和苯丙素代谢途径的影响。结果表明，0.04 mL L−1 BEO熏蒸能有效抑制灰葡萄球菌菌丝体的体外生长和孢子萌发，破坏细胞膜完整性，诱导细胞内蛋白质和核苷酸的渗漏。体内实验表明，0.04 mL L−1 BEO熏薰可通过提高淀粉酶、蔗糖合酶合成酶和蔗糖磷酸合酶活性及其相应基因表达，延缓可溶性糖和还原糖水平的下降，同时抑制酸性转化酶、中性转化酶、山梨醇氧化酶、蔗糖合酶裂解酶、nadp -山梨醇脱氢酶和nadp -山梨醇脱氢酶活性及其相应基因表达。此外，BEO熏蒸提高了苯丙氨酸解氨酶、4- coumaryl - coa连接酶、肉桂醇脱氢酶等与酚类生物合成相关的关键酶的酶活性，上调了基因表达水平。此外，BEO熏蒸显著提高了内源苯丙氨酸、对香豆酸、咖啡酸和辛酸的浓度，从而促进了蓝莓总酚类物质和木质素的积累。综上所见，BEO通过破坏菌膜的完整性，对灰葡萄球菌菌丝生长和孢子萌发产生直接抑制作用。此外，BEO熏蒸通过激活碳水化合物和苯丙素代谢途径的关键酶活性和基因表达，增加酚类化合物的积累，增强了蓝莓果实对灰霉病杆菌的抗性。

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引用次数: 0

FaERF6 activates the polygalacturonase gene FaPG1 to promote strawberry fruit softening FaERF6激活多半乳糖醛酸酶基因FaPG1，促进草莓果实软化

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-12-13 DOI: 10.1016/j.postharvbio.2025.114129

Yunhe Zhang , Shuo Wang , Xue Li , Kaiyi Cai , Hongying Sun , Zhihong Zhang

Postharvest softening in strawberry fruit substantially shortens shelf life and incurs significant economic losses; however, the regulatory networks governing this process remain poorly characterised. To elucidate these mechanisms, we integrated pectin quantification, cell wall ultrastructure analysis, and RNA-seq profiling of ‘Chulian’ (low-firmness) and ‘Yanli’ (high-firmness) cultivars across developmental and postharvest stages. This approach identified FaPG1 as a master regulator of pectin degradation during both preharvest and postharvest phases. Subsequent weighted gene co-expression network analysis coupled with yeast one-hybrid screening revealed FaERF6, an EAR-motif-containing dehydration-responsive element binding transcription factor. Functional validation confirmed FaERF6’s direct binding to and transcriptionally activating the FaPG1 promoter. Moreover, transient transformation assays demonstrated that FaPG1 overexpression induced accelerated softening and water-soluble pectin (WSP) accumulation. Subsequently, FaERF6 overexpression was shown to further activate FaPG1 transcription, leading to firmness reduction and increased WSP levels. Collectively, we establish a FaERF6-FaPG1 regulatory module that mechanistically explains DREB-mediated transcriptional control of cell wall remodelling during fruit softening.

草莓果实采后软化会大大缩短保质期，造成重大经济损失；然而，管理这一过程的监管网络仍然缺乏特征。为了阐明这些机制，我们整合了“Chulian”（低硬度）和“Yanli”（高硬度）品种在发育和收获阶段的果胶定量、细胞壁超微结构分析和RNA-seq分析。该方法确定FaPG1是采收前和采收后果胶降解的主要调节因子。随后的加权基因共表达网络分析结合酵母单杂交筛选发现FaERF6，一个含有ear基序的脱水响应元件结合转录因子。功能验证证实FaERF6直接结合并转录激活FaPG1启动子。此外，瞬时转化实验表明，FaPG1过表达会加速软化和水溶性果胶（WSP）的积累。随后，FaERF6过表达被证明进一步激活FaPG1转录，导致硬度降低和WSP水平升高。总之，我们建立了FaERF6-FaPG1调控模块，从机制上解释了dreb介导的果实软化过程中细胞壁重塑的转录控制。

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引用次数: 0

H₂S mitigates chilling injury by downregulating PpbHLH113 to enhance phenylpropanoid biosynthesis in peach fruit h2s通过下调PpbHLH113来促进桃果果实中苯丙素的合成，从而减轻了桃果的低温伤害

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2026-01-13 DOI: 10.1016/j.postharvbio.2026.114162

Yanyan Wang , Yaxuan Liu , Qingyuan Song , Tingyu Wu , Jiaqi Guo , Hao Dong , Kaili Shi , Xiaohui Yang , Yujun Shi , Siwen Yin , Jinbao Huang , Yuquan Duan , Li Wang

Postharvest chilling injury (CI) severely limits peach fruit quality. Hydrogen sulfide (H₂S) has shown potential in mitigating CI, while its underlying transcriptional regulation mechanism remains insufficiently characterized. This study revealed that H₂S treatment effectively mitigated CI by promoting both the enzymatic activities and transcript levels of key phenylpropanoid biosynthesis enzymes (PAL, C4H, 4CL, and CHI), accompanied by increased accumulation of total phenolics and flavonoids. Conversely, the H₂S scavenger hypotaurine reversed these effects in peach fruit. Notably, PpbHLH113, a nuclear-localized newly identified bHLH family member, was upregulated by cold stress and downregulated by H₂S. Moreover, functional studies revealed that PpbHLH113 overexpression downregulated the expression of phenylpropanoid pathway genes. Further molecular interaction assays confirmed direct binding of PpbHLH113 to E-box cis-elements in the promoters of Pp4CL and PpCHI. Although deficient in transcriptional activation, PpbHLH113 functioned as a transcriptional repressor in dual-luciferase assays, significantly inhibiting the promoter activity of Pp4CL and PpCHI, thereby leading to the suppression of phenylpropanoid biosynthesis. Collectively, H₂S treatment protected peach fruit from chilling injury by downregulating PpbHLH113, thereby alleviating its transcriptional repression on key phenylpropanoid pathway genes, maintaining high levels of key enzyme activities and phenolic accumulation, and improving antioxidant capacity and membrane integrity.

采后冷害严重影响桃果品质。硫化氢（H₂S）已显示出减轻CI的潜力，但其潜在的转录调控机制仍未充分表征。本研究表明，h2s处理通过提高关键苯丙素生物合成酶（PAL、C4H、4CL和CHI）的酶活性和转录物水平，同时增加总酚类物质和黄酮类物质的积累，有效缓解了CI。相反，在桃果中，H₂S清除剂次氨基磺酸逆转了这些作用。值得注意的是，新发现的bHLH家族成员PpbHLH113在冷胁迫下上调，在H₂S下下调。此外，功能研究显示PpbHLH113过表达下调了苯丙素途径基因的表达。进一步的分子相互作用分析证实PpbHLH113与Pp4CL和PpCHI启动子中的E-box顺式元件直接结合。虽然缺乏转录激活，但PpbHLH113在双荧光素酶实验中作为转录抑制因子，显著抑制Pp4CL和PpCHI的启动子活性，从而抑制苯丙素类生物合成。综上所述，h2s处理通过下调PpbHLH113，从而减轻其对苯丙素关键通路基因的转录抑制，维持高水平的关键酶活性和酚积累，提高抗氧化能力和膜完整性，从而保护桃果免于冷害。

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引用次数: 0

Fusaricidins from Paenibacillus polymyxa ZJU11 mediate the biological control of postharvest mango anthracnose 多粘类芽孢杆菌ZJU11的镰刀菌素介导芒果采后炭疽病的生物防治

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2026-01-02 DOI: 10.1016/j.postharvbio.2025.114148

Cui Sun , Yihan Wang , Yihu Pi , Jinping Cao , Yue Wang , Chaoyi Hu , Chongde Sun

Mango fruits are highly susceptible to infection by Colletotrichum species, leading to the severe economic losses. In this study, an antagonist of Paenibacillus polymyxa ZJU11 with strong biocontrol activity was identified and its antifungal mechanisms were investigated. P. polymyxa ZJU11 effectively suppressed the growth of pathogens of mango fruit in vitro and in vivo. The whole genome sequencing and LC-MS/MS results revealed that the active component in P. polymyxa ZJU11 exerting antifungal activity was Fusaricidin A, B, C and D. The cell membrane integrity of pathogens was demonstrated to be damaged after Fusaricidin extract treatment by PI staining, SEM and TEM observation. Additionally, transcriptomic analysis indicated Fusaricidin extract upregulated the gene expression in cell cycle, MAPK signaling pathway, and autophagy in C. asianum HIN6, induced cell death. This study provides a promising and suitable biocontrol agent to protect the postharvest mango fruit from fungal infection.

芒果果实极易受到炭疽病菌的侵染，造成严重的经济损失。本研究鉴定了一种具有较强生物防治活性的多粘类芽孢杆菌拮抗剂ZJU11，并对其抑菌机制进行了探讨。P. polymyxa ZJU11在体内外均能有效抑制芒果果实病原菌的生长。全基因组测序和LC-MS/MS结果显示，多粘菌ZJU11中发挥抗真菌活性的活性成分为Fusaricidin A、B、C和d。PI染色、SEM和TEM观察表明，Fusaricidin提取物处理后，病原菌细胞膜完整性被破坏。此外，转录组学分析表明，Fusaricidin提取物上调亚洲镰刀菌HIN6细胞周期、MAPK信号通路和自噬基因的表达，诱导细胞死亡。本研究为芒果果实采后真菌侵染提供了一种有前景的生物防治剂。

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引用次数: 0

Molecular and metabolic response of ‘Piccolo’ cherry tomato to Graduated Controlled Atmosphere ‘短笛’樱桃番茄对分级可控气氛的分子和代谢响应

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-12-29 DOI: 10.1016/j.postharvbio.2025.114133

Natalia Falagán, Roberta Tosetti, Ewan Gage, Leon A. Terry

Controlled atmosphere (CA) is used to extend the postharvest life of climacteric fruit by suppressing respiration and delaying ethylene mediated ripening. However, sudden exposure to low oxygen conditions can induce hypoxic stress, triggering metabolic and hormonal disruptions that negatively impact fruit quality. This study aimed to understand the mechanisms underlying ethylene sensitivity under low oxygen conditions through the application of a novel approach to CA, called Graduated Controlled Atmosphere (GCA), in ‘Piccolo’ cherry tomato. Compared to standard CA, GCA treatment resulted in greater suppression of respiration and improved firmness retention, indicating reduced physiological stress and slower cell wall degradation. Gene expression analysis revealed downregulation of NCED1, ACS, and ACO genes under GCA, indicating delayed ethylene-associated transcriptional activity. These hormonal adjustments were also reflected in lower abscisic acid (ABA) concentrations, implying a more stable ripening trajectory. Besides hormonal modulation, GCA-treated fruit exhibited alterations in primary metabolism. Sucrose accumulation and changes in malate levels under GCA conditions suggest a shift in energy metabolism, consistent with improved hypoxia tolerance. However, a notable trade-off was observed in reduced lycopene accumulation, potentially due to lower oxidative signalling and shared precursors between carotenoid and ABA biosynthesis. These findings demonstrate that GCA promotes a more controlled physiological and molecular response to hypoxic storage by reducing stress-associated metabolic and hormonal activity. GCA, as an advanced postharvest strategy, enhances texture retention and may reduce quality losses during storage. This work provides new mechanistic insights into hypoxia adaptation in fruit and supports the use of gradual atmosphere modification to optimise CA protocols.

控制气氛（CA）通过抑制呼吸作用和延迟乙烯介导的成熟来延长更年期果实的采后寿命。然而，突然暴露在低氧条件下会引起缺氧应激，引发代谢和激素紊乱，对水果品质产生负面影响。本研究旨在了解低氧条件下乙烯敏感性的机制，通过应用一种新的CA方法，称为渐次可控气氛（GCA），在‘ Piccolo ’樱桃番茄中。与标准CA相比，GCA治疗导致更大的呼吸抑制和硬度保持改善，表明生理应激减少，细胞壁降解减慢。基因表达分析显示，GCA下NCED1、ACS和ACO基因下调，表明乙烯相关转录活性延迟。这些激素调节也反映在较低的脱落酸（ABA）浓度上，这意味着更稳定的成熟轨迹。除了激素调节外，gca处理的果实还表现出初级代谢的改变。蔗糖积累和苹果酸水平在GCA条件下的变化表明能量代谢的转变，与缺氧耐受性的提高一致。然而，在减少番茄红素积累中观察到一个显著的权衡，可能是由于氧化信号的降低和类胡萝卜素和ABA生物合成之间共享的前体。这些发现表明，GCA通过降低与应激相关的代谢和激素活性，促进了对缺氧储存的更可控的生理和分子反应。GCA作为一种先进的采后策略，可以提高果实的质地保留，减少贮藏过程中的品质损失。这项工作为水果的缺氧适应提供了新的机制见解，并支持使用渐进的大气调节来优化CA协议。

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引用次数: 0

Gallic acid-grafted hardwood lignin: A bio-based antioxidant with enhanced biological properties 没食子酸接枝的硬木木质素：一种生物基抗氧化剂

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2026-01-20 DOI: 10.1016/j.postharvbio.2026.114166

Akhmadjon Sultanov , Eko Setio Wibowo , Byung-Dae Park , Young-Je Cho

A bio-based antioxidant was developed by grafting gallic acid (GA) onto hardwood lignin (HWL) via multi-step esterification. Industrial HWL, rich in aromatic phenylpropanoid units but deficient in free phenolic OH groups, was structurally modified to enhance radical-scavenging capacity. The synthesis involved acetylation of GA’s phenolic OH groups, conversion to acid chloride, esterification with HWL under basic catalysis, and deprotection to restore phenolic functionalities. ATR–FTIR confirmed loss of acetyl CO bands and intensified OH stretching; gel permeation chromatography indicated improved molecular homogeneity. Quantitative ³¹P NMR showed a fourfold increase in phenolic OH content (4.58–19.64 mmol/g) and near-complete conversion of aliphatic OH groups to esters. Antioxidant assays revealed 98.71 % DPPH scavenging and complete ABTS neutralization, outperforming native HWL and approaching the efficacy of butylated hydroxyanisole. GA-grafted HWL retained the lignin backbone and exhibited enhanced antioxidant properties, enabling lignin waste valorization and supporting sustainable applications for food as additives to improve antioxidant efficiency.

将没食子酸（GA）通过多步酯化接枝到硬木木质素（HWL）上，制备了一种生物基抗氧化剂。工业HWL富含芳香族苯丙类单位，但缺乏游离酚OH基团，通过结构修饰增强自由基清除能力。合成过程包括GA的酚羟基乙酰化，转化为酸性氯化物，在碱性催化下与HWL酯化，以及去保护以恢复酚功能。ATR-FTIR证实乙酰CO带丢失，OH拉伸增强；凝胶渗透色谱表明分子均匀性得到改善。定量31P核磁共振显示酚类OH含量增加4倍（4.58-19.64 mmol/g），脂肪族OH几乎完全转化为酯类。抗氧化实验显示，DPPH清除率为98.71% %，ABTS完全中和，优于天然HWL，接近丁基羟基茴香醇的效果。ga接枝的HWL保留了木质素骨架，并表现出增强的抗氧化性能，使木质素废物增值，并支持作为食品添加剂的可持续应用，以提高抗氧化效率。

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引用次数: 0

CgZAT11-driven lignin accumulation mediates juice sac granulation in postharvest pummelo (Citrus grandis L. Osbeck) cgzat11驱动的木质素积累介导采后柚汁囊造粒

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-11-25 DOI: 10.1016/j.postharvbio.2025.114090

Qiang Huang, Yingying Yang, Dan Peng, Xiawan Zhai, Zengyu Gan, Wenbin Kai, Chuying Chen, Jinyin Chen

Juice sac granulation is a complex physiological disorder that significantly compromises the eating quality and postharvest longevity of ‘Majiayou’ pummelo (Citrus grandis L.). The exact mechanisms by which juice sac granulation in citrus fruit remain elusive, particularly at the genetic regulatory level. In this study, we conducted a comprehensive transcriptomic analysis using RNA-seq data from juice sac samples collected at three granulation stages (G0, G1, and G2) of ‘Majiayou’ pummelo fruit, and characterized a nuclear-localizated C2H2 zinc finger protein, CgZAT11, whose expression progressively increased during juice sac granulation. Online prediction of CgZAT11 binding elements was used to scan the Citrus grandis genome, combining transcriptomic data to screen for eight potentially differentially expressed target genes involved in lignin biosynthesis. Functional validation revealed that CgZAT11 induced pronounced lignin accumulation and secondary cell wall thickening in pummelo juice sacs by enhancing the transcriptional levels of the CgCAD8 and CgPOD16 genes. Through integrated molecular assays—including yeast one-hybrid, dual-luciferase reporter, and electromobility shift assay—we demonstrated that CgZAT11 directly activates CgCAD8 and CgPOD16 by specifically binding to their promoters, thereby enhancing our understanding of the regulatory network governing juice sac granulation. These findings establish CgZAT11 as a transcriptional activator coordinating lignin biosynthesis pathways during juice sac granulation, providing novel mechanistic insights into the transcriptional regulation of juice sac granulation in ‘Majiayou’ pummelo fruit.

汁囊肉芽形成是一种复杂的生理失调，严重影响马家友柚的食性品质和采后寿命。柑桔汁囊颗粒化的确切机制仍然难以捉摸，特别是在遗传调控水平上。在本研究中，我们对“马家油”柚果在G0、G1和G2三个肉芽阶段的汁囊样本进行了RNA-seq数据的全面转录组学分析，并鉴定了一个核定位的C2H2锌指蛋白CgZAT11，其表达在汁囊肉芽形成过程中逐渐增加。利用CgZAT11结合元件的在线预测对柑橘基因组进行扫描，结合转录组学数据筛选8个参与木质素生物合成的潜在差异表达靶基因。功能验证表明，CgZAT11通过提高CgCAD8和CgPOD16基因的转录水平，诱导柚子汁囊木质素积累和二次细胞壁增厚。通过综合分子分析，包括酵母单杂交、双荧光素酶报告基因和电迁移转移分析，我们证明了CgZAT11通过特异性结合其启动子直接激活CgCAD8和CgPOD16，从而增强了我们对汁囊造粒调控网络的理解。这些发现证实了CgZAT11作为一个转录激活因子，在汁囊造粒过程中协调木质素的生物合成途径，为“马家油”柚汁囊造粒的转录调控机制提供了新的见解。

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引用次数: 0

PpbHLH59 is a key regulator of ethylene biosynthesis and cell wall degradation during peach fruit ripening and softening PpbHLH59是桃果实成熟和软化过程中乙烯生物合成和细胞壁降解的关键调控因子

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-11-28 DOI: 10.1016/j.postharvbio.2025.114095

Tongyang Kang , Yuting Xu , Zhexuan Peng , Xiaoyu Wang , Jieyu Dai , Ze Xu , Xingzhen Zhang , Qianjin Zhan , Zhengkun Guo , Yanan Hu , Caiping Zhao

Peach (Prunus persica) is a globally significant fruit crop, whereas its rapid ripening and softening processes bring great challenges for the storage and transportation. Therefore, it is of great significance to specify the mechanism of peach ripening and softening. The basic helix-loop-helix (bHLH) transcription factors (TFs) constitute one of the largest TF families in plants, and certain members have been implicated in the regulation of fruit ripening and softening. However, the modulatory roles of bHLH TFs in peach fruit ripening remain relatively understudied. In this study, a bHLH TF, PpbHLH59, was identified, and exhibited a high expression level during fruit development and ripening. Transient overexpression of PpbHLH59 in peach fruit clearly accelerated softening and elevated ethylene production, while its gene silencing delayed these ripening processes in turn. In peach callus, overexpression of PpbHLH59 led to significant upregulation of ripening and softening related genes, such as PpACO1, PpACS1, PpERF2a, PpPGM0, etc., whereas its silencing resulted in the opposite effects. Further biochemistry experiments confirmed that PpbHLH59 directly bound to the promoters of PpACO1, PpACS1, and PpPGM0, thereby activating their transcription and promoting fruit ripening and softening. Our studies established that PpbHLH59 acts as a key regulator of peach ripening and softening through coordinately regulating ethylene biosynthesis and cell wall degradation, which expands understanding of bHLH TFs in these biological processes.

桃（Prunus persica）是一种全球重要的水果作物，其快速的成熟和软化过程给储存和运输带来了巨大的挑战。因此，研究桃子成熟软化的机理具有重要意义。基本螺旋-环-螺旋（bHLH）转录因子（TFs）是植物中最大的转录因子家族之一，某些成员参与了果实成熟和软化的调控。然而，bHLH TFs在桃果成熟过程中的调节作用研究相对较少。本研究鉴定了一个bHLH TF， PpbHLH59，在果实发育和成熟过程中表现出高表达水平。PpbHLH59在桃果中的瞬时过表达明显加速了桃果的软化和乙烯的产生，而其基因沉默则反过来延迟了这些成熟过程。在桃愈伤组织中，PpbHLH59的过表达导致PpACO1、PpACS1、PpERF2a、PpPGM0等成熟和软化相关基因的显著上调，而其沉默则导致相反的效果。进一步的生化实验证实PpbHLH59直接结合PpACO1、PpACS1和PpPGM0的启动子，激活它们的转录，促进果实成熟和软化。我们的研究证实PpbHLH59通过协调调节乙烯生物合成和细胞壁降解，是桃子成熟和软化的关键调节剂，这扩大了对bHLH TFs在这些生物过程中的理解。

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引用次数: 0

Efficacy and possible mechanism of trans-2-octenal in controlling grey mould caused by Botrytis cinerea on harvested fruit 反式-2-辛醛防治果实灰霉病的效果及可能机制

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-11-29 DOI: 10.1016/j.postharvbio.2025.114101

Jiamei Yuan , Jie Yang , Yushan Du , Liwenqian Wang , Fengjun Wang , Guangjin Li

The volatile organic compounds derived from plants are increasingly utilized in postharvest disease management owing to their potent antifungal properties. The present study examined the antifungal effects of trans-2-octenal (OCT), a natural volatile compound, against Botrytis cinerea and explored its mode of action. OCT demonstrated strong antifungal activity in vitro, completely inhibiting mycelial growth at 4 μL L⁻¹ and significantly impairing conidial germination and germ tube elongation of B. cinerea. In vivo studies revealed that OCT reduced grey mould severity while maintained fruit quality during storage. Transcriptomic analysis indicated that OCT interfered with multiple cellular processes, particularly ribosome biosynthesis, stress response-related pathways and the expression of pathogenicity-associated genes including Bcbot1–5, Bcoah, Bcpg4, Bmp1, Bcxyn11A and NADPH oxidase complex. More notably, OCT disrupted the membrane lipid metabolism and damaged the membrane integrity, resulting in cytoplasmic content leakage and deleterious morphological modifications. Collectively, these results elucidate the antifungal activity and mode of action of OCT against B. cinerea, highlighting its promise for controlling grey mould on harvested produce.

来自植物的挥发性有机化合物由于其有效的抗真菌特性而越来越多地用于采后病害管理。本文研究了天然挥发性化合物反式-2-辛烯醛（OCT）对灰葡萄孢（Botrytis cinerea）的抑菌作用，并探讨了其作用机理。OCT在体外表现出较强的抗真菌活性，在4 μL L−1浓度下完全抑制菌丝生长，并显著抑制灰葡萄孢子萌发和芽管伸长。体内研究表明OCT降低了灰霉病的严重程度，同时在储存期间保持了水果的品质。转录组学分析表明，OCT干扰了多种细胞过程，特别是核糖体生物合成、应激反应相关途径以及bbot1 - 5、Bcoah、Bcpg4、Bmp1、Bcxyn11A和NADPH氧化酶复合物等致病性相关基因的表达。更值得注意的是，OCT破坏了膜脂代谢，破坏了膜的完整性，导致细胞质内容物泄漏和有害的形态改变。总的来说，这些结果阐明了OCT对灰霉菌的抗真菌活性和作用方式，突出了其在控制收获农产品灰霉病方面的前景。

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引用次数: 0

Alpha-ketoglutarate alleviates chilling injury in banana fruit by promoting redox homeostasis and maintaining energy balance α -酮戊二酸通过促进氧化还原稳态和维持能量平衡来减轻香蕉果实的冷害

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-11-24 DOI: 10.1016/j.postharvbio.2025.114075

Yuemin Yang , Rufang Deng , Yueming Jiang , Xuewu Duan , Zhengke Zhang , Guoxiang Jiang , Mengting Liu

Banana fruit is susceptible to chilling injury (CI) under low-temperature stress, leading to quality deterioration. Although alpha-ketoglutarate (AKG) has been extensively studied for its role in cellular homeostasis, its effects on postharvest fruit and preservation mechanisms remain unclear. This study demonstrates that AKG treatment alleviates CI in banana fruit by preserving phenolic compounds, ascorbic acid (AsA) and glutathione (GSH) content while enhancing antioxidant enzyme activity—including catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX). Concurrently, AKG maintained lower H₂O₂ and O₂^•– levels. Furthermore, AKG sustained the activity of key tricarboxylic acid (TCA) cycle enzymes (succinate dehydrogenase (SDH), α-ketoglutarate dehydrogenase (α-KGDH) and malate dehydrogenase (MDH), promoting higher ATP levels and energy charge (EC). Transcriptome analysis revealed that AKG modulates genes associated with oxidoreductase activity, small molecule transport, glycosyltransferase function, mitochondrial energy metabolism and antioxidant pathways. RT-qPCR validation confirmed significant upregulation of key cold-responsive genes (MaAOX1a, MaRbohH, MaHSP22, MaPLD1, MaPAL and MaMYB4). Collectively, these findings suggest that AKG alleviates CI in banana fruit by simultaneously improving redox homeostasis and preserving mitochondrial energy metabolism.

香蕉果实在低温胁迫下易发生冷害，导致品质劣化。虽然α -酮戊二酸（AKG）在细胞稳态中的作用已被广泛研究，但其对采后果实的影响及其保存机制尚不清楚。本研究表明，AKG处理通过保持香蕉果实酚类化合物、抗坏血酸（AsA）和谷胱甘肽（GSH）含量，同时提高过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、过氧化物酶（POD）和抗坏血酸过氧化物酶（APX）的抗氧化酶活性，缓解了香蕉果实CI。同时，AKG维持较低的H₂O₂和O2•水平。此外，AKG还维持了三羧酸（TCA）关键循环酶(琥珀酸脱氢酶（SDH）、α-酮戊二酸脱氢酶（α-KGDH）和苹果酸脱氢酶（MDH）的活性，提高了ATP水平和能量电荷（EC）。转录组分析显示，AKG调节与氧化还原酶活性、小分子转运、糖基转移酶功能、线粒体能量代谢和抗氧化途径相关的基因。RT-qPCR验证证实了关键冷应答基因（MaAOX1a、MaRbohH、MaHSP22、MaPLD1、MaPAL和MaMYB4）的显著上调。综上所述，这些发现表明AKG通过同时改善氧化还原稳态和保持线粒体能量代谢来减轻香蕉果实中的CI。

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