Postharvest Biology and Technology最新文献_第3页

Multi-targeted antifungal mechanism of punicalagin against Botrytis cinerea and its role in preserving strawberry quality 甘薯苷对葡萄灰霉病的多靶点抗真菌机理及其对草莓品质的保护作用

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-19 DOI: 10.1016/j.postharvbio.2026.114156

Muhammad Nawaz , Junkun Pan , Hui Liu , Keying Feng , Jiechao Liu , Wenbo Yang , Zhenzhen Lv , Qiang Zhang , Zhonggao Jiao

Gray mold, caused by the fungus Botrytis cinerea, is a key postharvest disease of strawberries, resulting in substantial fruit rot and economic losses. This study elucidated the comprehensive antifungal mechanism of punicalagin against B. cinerea. The antifungal action of punicalagin primarily involved the disruption of fungal cell membrane integrity, as evidenced by the induction of cytoplasmic leakage, accumulation of malondialdehyde and reduction in ergosterol and trehalose. Ultrastructural analysis revealed severe hyphal damage after punicalagin treatment. Punicalagin also induced apoptosis through mitochondrial membrane dysfunction and the accumulation of reactive oxygen species (ROS). Furthermore, transcriptomic analysis identified 2589 downregulated and 1157 upregulated genes in punicalagin-treated mycelia, with a particular impact on membrane lipid and carbohydrate metabolism pathways. These transcriptomic alterations, representing impaired energy production and antioxidant systems, were further validated by corresponding metabolomic analysis. Additionally, punicalagin treatment preserved the quality of strawberry fruit by improving cell wall structure to maintain firmness and augmenting phenolic content, potentially enhancing resistance. These multifaceted mechanisms collectively contributed to the exceptional efficacy of punicalagin in controlling B. cinerea. These findings suggest that punicalagin could be a promising natural antifungal alternative, offering effective control of gray mold and maintaining the quality of strawberries in storage.

灰霉病是草莓采后主要病害，由葡萄灰霉病（Botrytis cinerea）引起，造成大量果实腐烂和经济损失。本研究阐明了槟榔苷对灰霉病菌的综合抑菌机制。punicalagin的抗真菌作用主要涉及破坏真菌细胞膜的完整性，这可以通过诱导细胞质渗漏、丙二醛积累和麦角甾醇和海藻糖减少来证明。超微结构分析显示，针刺后菌丝损伤严重。Punicalagin还通过线粒体膜功能障碍和活性氧（ROS）的积累诱导细胞凋亡。此外，转录组学分析发现，在槟榔素处理的菌丝体中，有2589个下调基因和1157个上调基因，对膜脂和碳水化合物代谢途径有特别的影响。这些转录组改变，代表了能量产生和抗氧化系统的受损，通过相应的代谢组学分析进一步验证。此外，槟榔苷处理通过改善细胞壁结构以保持果实硬度和增加酚类含量来保持草莓果实的品质，从而有可能增强抗性。这些多方面的机制共同促成了槟榔苷在控制葡萄球菌方面的特殊功效。这些结果表明，槟榔苷可能是一种有前景的天然抗真菌替代品，可以有效地控制草莓的灰霉病，并保持草莓的储存质量。

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引用次数: 0

PpMYC2 positively regulates chilling tolerance of peach fruit by suppressing sucrose degradation PpMYC2通过抑制蔗糖降解正向调节桃果的抗寒性

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-19 DOI: 10.1016/j.postharvbio.2026.114179

Qian Li , Yingying Wei , Yi Chen , Shu Jiang , Jianfen Ye , Fangming Wu , Feng Xu , Xingfeng Shao

MYC2 transcription factor has been extensively investigated in plant stress responses. However, its role in regulating fruit cold tolerance remains largely unexplored. In this study, exposure to cold stress rapidly activated the transcription of PpMYC2 in peach fruit. DNA affinity purification sequencing (DAP-seq) data revealed that PpMYC2 showed high affinity for the promoter region of PpVIN2 - a key gene encoding acidic vacuolar invertase (VIN) critical for sucrose catabolism in peach fruit. Yeast one-hybridization (Y1H), electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter (DLR) assay collectively revealed that PpMYC2 directly bound to the G-box cis-element in the PpVIN2 promoter and significantly suppressed its promoter activity. Transient overexpression of PpMYC2 in peach fruit resulted in downregulated transcription of PpVIN2, reduced VIN activity, and increased sucrose content, whereas opposite changes were detected in peach fruit with transient silencing of PpMYC2. Additionally, we generated tomato plants stably overexpressing PpMYC2 using Agrobacterium-mediated transformation, and observed that PpMYC2-overexpressing tomato fruit exhibited significantly enhanced cold tolerance, accompanied by the inhibition of sucrose degradation. Collectively, these discoveries indicate that PpMYC2 acts as a transcriptional repressor of PpVIN2 under cold stress, thereby reducing sucrose breakdown and enhancing peach fruit chilling tolerance. This study identifies PpMYC2 as a potential molecular target for alleviating chilling injury in peach fruit.

MYC2转录因子在植物逆境应答中被广泛研究。然而，它在调节水果耐寒性方面的作用仍未得到充分研究。在本研究中，暴露于冷胁迫下的桃果快速激活PpMYC2的转录。DNA亲和纯化测序（DAP-seq）数据显示，PpMYC2对PpVIN2的启动子区域具有高亲和性，PpVIN2是桃子果实蔗糖分解代谢关键的酸性液泡转化酶（VIN）的关键基因。酵母单杂交（Y1H）、电泳迁移率转移（EMSA）和双荧光素酶报告基因（DLR）实验共同发现PpMYC2直接结合PpVIN2启动子中的G-box顺式元件，并显著抑制其启动子活性。桃果实中PpMYC2的瞬时过表达导致PpVIN2转录下调，VIN活性降低，蔗糖含量增加，而PpMYC2的瞬时沉默则导致相反的变化。此外，我们还利用农杆菌介导的转化方法稳定地培养了过表达PpMYC2的番茄植株，并观察到过表达PpMYC2的番茄果实具有显著增强的耐寒性，同时抑制了蔗糖的降解。综上所述，这些发现表明PpMYC2在低温胁迫下作为PpVIN2的转录抑制因子，从而减少蔗糖分解，增强桃果的抗寒性。本研究确定PpMYC2是缓解桃果冷害的潜在分子靶点。

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引用次数: 0

Biocontrol and ecophysiological characterization of bacterial and yeast BCA candidates isolated from two different environments to control postharvest diseases 从两种不同环境中分离的细菌和酵母BCA候选物防治采后病害的生物防治和生态生理特性

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-19 DOI: 10.1016/j.postharvbio.2026.114174

Ana María Sánchez , Cristina Solsona , Jonàs Oliva , Neus Teixidó

Biocontrol agents are a sustainable alternative to chemical fungicides in postharvest protection. Part of their success depends on using strains able to persistently colonize the host and maintain a broad activity from the field to the storage facilities. In this report, we evaluated 213 potential biocontrol agents (124 bacteria, 78 yeasts, and 11 filamentous fungi) isolated from two contrasting environments, the Pyrenees Mountains and the Ebro Valley (Catalonia, Spain). Their efficacy was tested against the three main postharvest pathogens of apples: Penicillium expansum, Botrytis cinerea, and Rhizopus stolonifer. The results showed that 4 bacteria and 25 yeast strains were effective against these pathogens, reducing their incidence by more than 75 %. From these results, two Pantoea agglomerans and two Vishniacozyma carnescens were further studied in terms of growth at different temperatures and water availability in vitro and their persistence on apple surfaces. The ecophysiological characterization showed that the valley P. agglomerans strain tolerated lower water activities better than the mountain strain, suggesting adaptation to the region’s hot and dry climate. In contrast, the mountain V. carnescens performed better at low temperatures, while the valley strain showed superior growth at 30 ºC, consistent with their respective environmental origins. In conclusion, these results indicated the need to consider the environmental adaptability of potential biocontrol agents when searching for new BCA strains.

生物防治剂是化学杀菌剂在采后保护中的可持续替代品。它们的成功部分取决于使用能够持续定植宿主并从田间到储存设施保持广泛活动的菌株。在本报告中，我们评估了从比利牛斯山脉和埃布罗河谷（西班牙加泰罗尼亚）两个不同环境中分离的213种潜在的生物防治剂（124种细菌，78种酵母和11种丝状真菌）。对苹果采后3种主要病原菌：扩张青霉、灰霉病菌和匍匐茎霉进行了抑菌效果试验。结果表明，4种细菌和25株酵母菌对这些病原菌有效，使其发病率降低了75% %以上。在此基础上，进一步研究了两种Pantoea凝集菌和两种Vishniacozyma carnescens在不同温度和水分条件下的体外生长及其在苹果表面的持久性。生态生理特征表明，谷地团聚单胞菌对水分活动的耐受性优于山地单胞菌，表明其对该地区干热气候的适应。相比之下，山地V. carnescens在低温下表现较好，而山谷菌株在30℃下表现较好，这与它们各自的环境起源一致。综上所述，在寻找新的BCA菌株时，需要考虑潜在生物防治剂的环境适应性。

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引用次数: 0

Pyrazine-2-carboxylic acid delays juice sac granulation in pummelo through CgWRKY48-mediated repression of CgCAD1 吡嗪-2-羧酸通过cgwrky48介导的CgCAD1抑制延缓柚汁囊造粒

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-19 DOI: 10.1016/j.postharvbio.2026.114170

Jin-mei Chang , Ming-zhe Yang , Yan-xue Guo , Pan-pan Tong , Tian-qi Guo , Gui-zhen Gao , Jian-ye Chen , Lu-bin Zhang , Hai-hua Luo , Wei Wei

Postharvest lignification-induced juice sac granulation significantly deteriorates the quality of pummelo fruit. We identify pyrazine-2-carboxylic acid (PCA) as an effective preservative that maintains both sensory and biochemical attributes during storage, including the inhibition of lignin accumulation, preservation of juice sac integrity, protection of sugars (glucose, sucrose and fructose) and organic acids (tartaric acid and malic acid), and reduction of CAD and PAL activities. Transcriptomic analysis revealed that PCA suppresses phenylpropanoid biosynthesis, notably through the downregulation of CgCAD1, a key gene encoding cinnamyl alcohol dehydrogenase. Furthermore, CgWRKY48 was identified as a nucleus-localized transcriptional repressor that directly binds to the CgCAD1 promoter and suppresses its transcription. Transient overexpression assays in Tobacco showed that CgWRKY48 suppresses lignin accumulation and the expression of phenylpropanoid-pathway genes, whereas CgCAD1 exhibits the opposite effect. This paper establishes a regulatory module that modulates lignin biosynthesis during fruit senescence, offering promising molecular targets for postharvest quality preservation in citrus fruits.

采后木质素化引起的汁囊粒化会显著降低柚果品质。我们发现吡嗪-2-羧酸（PCA）是一种有效的防腐剂，可以在储存过程中保持感官和生化特性，包括抑制木质素积累，保持汁囊完整性，保护糖（葡萄糖、蔗糖和果糖）和有机酸（酒石酸和苹果酸），降低CAD和PAL活性。转录组学分析显示，PCA通过下调编码肉桂醇脱氢酶的关键基因CgCAD1来抑制苯丙醇类生物合成。此外，CgWRKY48被鉴定为细胞核定位的转录抑制因子，可直接结合CgCAD1启动子并抑制其转录。烟草中的瞬时过表达实验表明，CgWRKY48抑制木质素积累和苯丙素途径基因的表达，而CgCAD1则表现出相反的作用。本文建立了一个调控果实衰老过程中木质素生物合成的调控模块，为柑橘果实采后品质保鲜提供了有前景的分子靶点。

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引用次数: 0

Ethephon and alternating magnetic field inhibit the browning of fresh-cut stem lettuce by regulating phenolic and reactive oxygen species metabolism 乙烯利和交变磁场通过调节酚类物质和活性氧代谢抑制鲜切茎生菜褐变

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-19 DOI: 10.1016/j.postharvbio.2026.114173

Tuankui Ou , Mengsheng Deng , Yingmei Tao , Huina Li , Wenao Sun , Xingyong Zhang , Dong Li

Enzymatic browning significantly reduces the quality of fresh-cut stem lettuce. This study evaluated the combined effect of ethephon (ET) and alternating magnetic field (AMF) on enzymatic browning, and clarified the inhibition mechanism involved in fresh-cut stem lettuce browning. The results indicated that the ET+AMF combination exhibited the best inhibitory effect on the browning of fresh-cut stem lettuce, compared to both the control and individual treatments of ET or AMF. ET+AMF effectively retarded the decline of quality parameters, including firmness, soluble solids, and ascorbic acid, and modulated key physiological processes by suppressing the respiration rate and mitigating weight and chlorophyll loss. Further investigation suggested that ET+AMF downregulated phenylalanine ammonia-lyase and polyphenol oxidase activities, resulting in the reduced accumulation of phenolics, including chlorogenic, caffeic, ferulic, and protocatechuic acid, and reducing quinone formation. Meanwhile, ET+AMF coordinately accelerated reactive oxygen species scavenging, primarily by augmenting the activities of peroxidase, superoxide dismutase, catalase and ascorbate peroxidase, and ultimately maintained the cell membrane integrity of fresh-cut stem lettuce, as evidenced by reduced relative electrolyte leakage and malondialdehyde content. These findings demonstrated that ET and AMF combined to inhibit stem lettuce browning by dual-regulating phenolic metabolism and reactive oxygen species (ROS) homeostasis, offering a novel strategy for controlling enzymatic browning.

酶促褐变显著降低了鲜切生菜的品质。本研究考察了乙烯利（ET）和交变磁场（AMF）对生菜酶促褐变的联合作用，阐明了其抑制鲜切生菜褐变的机制。结果表明，与ET或AMF单独处理和对照处理相比，ET+AMF联合处理对鲜切茎生菜褐变的抑制效果最好。ET+AMF有效延缓了硬度、可溶性固形物和抗坏血酸等品质参数的下降，并通过抑制呼吸速率、减轻体重和叶绿素损失来调节关键生理过程。进一步研究表明，ET+AMF下调苯丙氨酸解氨酶和多酚氧化酶活性，导致绿原、咖啡酸、阿魏酸和原儿茶酸等酚类物质的积累减少，并减少醌的形成。同时，ET+AMF主要通过提高过氧化物酶、超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶的活性，协同加速活性氧的清除，最终维持鲜切茎生菜细胞膜的完整性，减少相对电解质泄漏和丙二醛含量。这些结果表明，ET和AMF通过双调控酚类代谢和活性氧（ROS）稳态共同抑制茎叶莴苣褐变，为控制酶促褐变提供了一种新的策略。

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引用次数: 0

Detection of early decay in dynamic oranges by structured-illumination reflectance imaging combined with Hough gradient method and YOLO v10 model 结合Hough梯度法和YOLO v10模型的结构照明反射成像检测动态橙子的早期衰减

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-19 DOI: 10.1016/j.postharvbio.2026.114177

Yizhi Zhang , Zhonglei Cai , Sijie Zhou , Junyi Zhang , Hailiang Zhang , Jiangbo Li

Early decay induced by fungal infections in citrus fruit can lead to severe economic losses. In order to detect early decayed oranges in a moving state, this study proposed a new methodology based on structured illumination reflectance imaging (SIRI), which uses a fixed phase stripe pattern for illumination and introduces phase shift into the obtained pattern images through sample motion. The Hough gradient method was used to align the regions of interest in acquired pattern images. The direct component (DC) and alternating component (AC) images were recovered by using the 2-phase spiral phase transition (SPT) demodulation algorithm. At a speed of 100 mm/s, the combination of AC images and YOLO v10 achieved the highest classification accuracy, which was over 97 %. This study indicated that the proposed methodology provided a valuable reference to the online detection of early decayed oranges.

柑橘类水果真菌感染引起的早期腐烂会导致严重的经济损失。为了检测运动状态下的早期腐烂橙子，本研究提出了一种基于结构照明反射成像（SIRI）的新方法，该方法使用固定相位条纹模式进行照明，并通过样本运动在获得的模式图像中引入相移。利用霍夫梯度法对获取的模式图像中感兴趣的区域进行对齐。采用两相螺旋相变（SPT）解调算法恢复直接分量（DC）和交流分量（AC）图像。在100 mm/s的速度下，AC图像与YOLO v10组合的分类准确率最高，达到97 %以上。研究结果表明，所提出的方法为早期腐烂橘子的在线检测提供了有价值的参考。

引用次数: 0

Harnessing the microbiome to decipher the biocontrol mechanism of Serratia rubidaea B11 and develop an industrial formulation against pear blue mold 利用微生物组研究红沙雷氏菌B11的生物防治机制，开发抗梨蓝霉菌的工业配方

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-19 DOI: 10.1016/j.postharvbio.2026.114169

Kaili Wang , Xiaowen Ye , Tengxia Pan , Hua Wang , Yuchun Lu , Esa Abiso Godana , Qiya Yang , Asanda Mditshwa , Hongbin Chen , Hongyin Zhang

The postharvest pathogen Penicillium expansum causes substantial economic losses in pears. The antagonistic bacterium Serratia rubidaea B11 was isolated from orchard soil and characterized as an effective, non-toxic biocontrol agent. Physiological and microbiome analyses revealed a dual-pathway mechanism: S. rubidaea B11 directly inhibited pathogen growth and induced host systemic resistance by enhancing defense enzyme activities such as APX (ascorbate peroxidase), POD (peroxidase), PAL (phenylalanine ammonia-lyase), PPO (polyphenol oxidase), and SOD (superoxide dismutase). In addition to promoting resistant compound accumulation, it also reduced the oxidative stress of pears. Furthermore, S. rubidaea B11 altered the fruit surface microbiome, enriching beneficial taxa and suppressing pathogens. A stable formulation was developed, maintaining high viability and efficacy across storage temperatures. This work confirms S. rubidaea B11's potential as a commercial, eco-friendly biocontrol agent, highlighting the promise of harnessing the fruit microbiome for sustainable postharvest disease management.

采后病原菌扩张青霉对梨造成了巨大的经济损失。从果园土壤中分离到拮抗细菌红沙雷氏菌B11，是一种有效、无毒的生物防治剂。生理学和微生物组学分析揭示了其双通路机制：rubbidaea B11通过提高抗坏血酸过氧化物酶（APX）、过氧化物酶（POD）、苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）和超氧化物歧化酶（SOD）等防御酶活性，直接抑制病原菌生长，诱导宿主产生系统性抗性。除促进抗性化合物积累外，还能降低梨的氧化应激。此外，rubidaea B11改变了果实表面微生物群，丰富了有益类群并抑制了病原体。开发了一种稳定的配方，在储存温度下保持高活力和功效。这项研究证实了红葡萄球菌B11作为一种商业的、环保的生物防治剂的潜力，突出了利用果实微生物群进行可持续采后疾病管理的前景。

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引用次数: 0

DlNAC41 and DlNAC100 transcription factors modulate longan pulp breakdown via adjusting the expression of cell wall degradation-related genes DlNAC41和DlNAC100转录因子通过调控细胞壁降解相关基因的表达调控龙眼果肉降解

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-19 DOI: 10.1016/j.postharvbio.2026.114165

Hui Wang , Wenlong Zhou , Xinyue Mu , Zhongqi Fan , Ruiling Zhuo , Yihui Chen , Wangjin Lu , Boqiang Li , Shiping Tian , Yifen Lin , Hetong Lin

Pulp breakdown is a pivotal problem affecting the quality of postharvest longan fruit. However, regulatory mechanisms underlying the breakdown development process of longan pulp are still poorly understood. In this study, five NAC transcription factors (DlNAC8/17/41/79/100) were identified to be involved in longan pulp breakdown according to analysis of transcriptome sequencing results and RT-qPCR assay. In addition, 2,4-dinitrophenol (DNP) treatment reduced the levels of ADP, ATP and energy charge in longan pulp, up-regulated the expression of DlNAC8/17/41/79/100, increased activities of cell wall degradation-related enzymes (PME, XET, Cx, β-Gal and PG) and their corresponding genes, accelerated the degradations of cell wall components, resulting in aggravated longan pulp breakdown. Whereas, ATP treatment displayed the opposite effects. Furthermore, the subcellular localization assay and transactivation assays indicated that DlNAC41 and DlNAC100 were global-localized activators. The results of dual-luciferase reporter (DLR) assay and yeast one-hybrid (Y1H) assay suggested that DlNAC41 and DlNAC100 could directly bind to promoters of DlPME1/2, DlPG1/2 and Dlβ-Gal1. In addition, the transient overexpression of DlNAC41 and DlNAC100 up-regulated the transcription expressions of DlPME1/2/3, DlPG1/2, Dlβ-Gal1, DlCx1 and DlXET30, aggravated pulp breakdown of postharvest longans, implying the promoting roles of DlNAC41 and DlNAC100 in regulating development of lognan pulp breakdown. This discovery established a DlNACs-cell wall degradation-energy regulatory network unravelling the molecular mechanisms of longan pulp breakdown, and offering a new basis for restraining pulp breakdown of postharvest longans.

果肉破碎是影响桂圆采后果实品质的关键问题。然而，龙眼纸浆破碎发育过程的调控机制尚不清楚。本研究通过转录组测序结果分析和RT-qPCR检测，鉴定出5个NAC转录因子（DlNAC8/17/41/79/100）参与龙眼髓分解。此外，2,4-二硝基苯酚（DNP）处理降低了龙眼果肉中ADP、ATP和能量电荷的水平，上调了DlNAC8/17/41/79/100的表达，增加了细胞壁降解相关酶（PME、XET、Cx、β-Gal和PG）及其相应基因的活性，加速了细胞壁成分的降解，导致龙眼果肉分解加剧。而ATP处理则表现出相反的效果。此外，亚细胞定位实验和转激活实验表明，DlNAC41和DlNAC100是全局定位的激活剂。双荧光素酶报告基因（DLR）检测和酵母单杂交（Y1H）检测结果表明，DlNAC41和DlNAC100可以直接结合DlPME1/2、DlPG1/2和Dlβ-Gal1的启动子。此外，DlNAC41和DlNAC100的过表达上调了DlPME1/2/3、DlPG1/2、Dlβ-Gal1、DlCx1和DlXET30的转录表达，加重了采后龙眼的髓质破裂，说明DlNAC41和DlNAC100在调节龙眼髓质破裂发育中起促进作用。该发现建立了dlnacs -细胞壁降解-能量调控网络，揭示了桂圆纸浆分解的分子机制，为抑制采后桂圆纸浆分解提供了新的依据。

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引用次数: 0

Mechanism of curcumin mediated carbon cycle network and energy metabolism to improve the storage quality and flavor of pepper 姜黄素介导的碳循环网络和能量代谢改善辣椒贮藏品质和风味的机制

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-17 DOI: 10.1016/j.postharvbio.2026.114164

Wenjuan Xu , Yansheng Wang , Chengli Jia , Zhiqing Gong , Jian Zhang , Fengjuan Jia , Wenjia Cui , Shasha Song , Xiaochun Qin , Wenliang Wang , Junyan Shi

Curcumin (CUR) is a natural food preservative known for its various biological activities. This study systematically examined its effects on the physiological quality and flavor characteristics of postharvest chili peppers. The results showed that treating the peppers with 2 g L⁻¹ CUR effectively delayed fruit reddening, preserved nutritional quality, and inhibited postharvest deterioration. A significant enhancement in richness was observed in the CUR group via the electronic tongue compared to the CK group. Additionally, non-targeted volatile compound analysis conducted through gas chromatography-mass spectrometry (GC-MS) revealed that CUR significantly increased the abundance of 35 volatile flavor compounds relative to the CK group. Relative Odor Activity Value (ROAV) analysis highlighted 2-methoxy-3-(2-methylpropyl) pyrazine as the key characteristic flavor compound found in the CUR group, which was absent in the CK group. This observation confirms the role of CUR in shaping the key flavor profiles of chili peppers. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that most of the differential volatile substances were enriched in the core metabolic pathways associated with carbon cycling, including glycolysis/gluconeogenesis and pyruvate metabolism. These findings illuminate the potential metabolic mechanisms by which CUR enhances flavor quality in chili peppers from a pathway perspective. Overall, this study provides a theoretical basis for using CUR as a natural agent that offers both preservative and flavor-enhancing properties in the postharvest preservation of fruits and vegetables.

姜黄素（Curcumin， CUR）是一种天然的食品防腐剂，具有多种生物活性。本研究系统地考察了其对采后辣椒生理品质和风味特性的影响。结果表明，2 g L−1 CUR处理能有效延缓果实变红，保留营养品质，抑制采后变质。与CK组相比，通过电子舌观察到CUR组丰富度的显著增强。此外，通过气相色谱-质谱（GC-MS）进行的非靶向挥发性化合物分析显示，相对于CK组，CUR显著增加了35种挥发性风味化合物的丰度。相对气味活性值（ROAV）分析显示，2-甲氧基-3-（2-甲基丙基）吡嗪是CUR组中发现的关键特征风味化合物，而CK组中没有。这一观察证实了CUR在形成辣椒的关键风味特征中的作用。此外，京都基因与基因组百科（KEGG）途径富集分析表明，大多数差异挥发性物质富集于与碳循环相关的核心代谢途径，包括糖酵解/糖异生和丙酮酸代谢。这些发现从途径的角度阐明了CUR提高辣椒风味质量的潜在代谢机制。综上所述，本研究为将CUR作为一种既具有防腐性能又具有增味性能的天然试剂用于果蔬采后保鲜提供了理论基础。

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引用次数: 0

Comprehensive lipidomics analysis reveals the ceramides play an essential role in chilling injury of bell peppers 综合脂质组学分析表明，神经酰胺在甜椒冷害中起重要作用

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-17 DOI: 10.1016/j.postharvbio.2026.114171

Yingbo Zhao, Zhe Zhang, Manli Luo, Shujuan Ji

Low-temperature storage and transportation after harvesting can effectively maintain the quality of bell peppers, but inappropriate low temperatures can cause fruit chilling injury. The changes in membrane lipid components at low temperatures are the leading cause. Screening the main changed lipid components is beneficial for further studying the mechanism. In this study, 1310 lipid components were detected in the flesh of bell peppers after being stored at 4 ℃ and 10 ℃ for 25 d, including glycerophospholipids (47.39 %), ceramides (17.31 %), glycerides (14.36 %), glycerolipids (11.64 %), betaine lipids (2.14 %), lysophosphatides (0.37 %), and other lipids (6.79 %). Multivariate statistical analysis revealed the lipid components of the 4 ℃ and 10 ℃ groups contained 158 differential lipids, with 80 lipids upregulated and 78 lipids downregulated in the 4 ℃ group. The OPLS multiple regression analysis of differential lipids and chilling injury apparent indicators showed that 8 ceramides, including Cer ADS d22:0/19:0, Cer ADS d26:0/14:0, Cer ADS d17:0/19:0, Cer AS d14:3/22:2, Cer AS d18:2/18:2, Cer AP d14:0/22:2, Cer AP d14:2/32:2, HexCer AP t18:0/16:0, are more closely related to low-temperature stress. It indicated that ceramides are the key metabolites under low-temperature stress in bell peppers. Further correlation analysis between the content of 8 key ceramides and the relative conductivity revealed that Cer ADS d17:0/19:0 can serve as a preliminary biomarker to predict the occurrence of chilling injury in bell peppers. The results provide clues and a basis for further studying the mechanism of chilling injury of bell pepper under low temperature and exploring the corresponding control technology.

采后低温储运能有效保持甜椒品质，但低温不适宜会造成果实冷害。低温下膜脂组分的变化是主要原因。筛选主要改变的脂质组分有利于进一步研究其作用机制。在4℃和10℃贮藏25 d后，在甜椒果肉中检测到1310种脂质成分，包括甘油磷脂（47.39 %）、神经酰胺（17.31 %）、甘油酯（14.36 %）、甘油脂（11.64 %）、甜菜碱脂（2.14 %）、溶血磷脂（0.37 %）和其他脂质（6.79 %）。多因素统计分析显示，4℃组和10℃组的脂质成分中含有158种差异脂质，其中4℃组80种脂质上调，78种脂质下调。不同脂质和冻害表观指标的ops多元回归分析表明，Cer ADS d22:0/19:0、Cer ADS d26:0/14:0、Cer ADS d17:0/19:0、Cer AS d14:3/22:2、Cer AS d18:2/18:2、Cer AP d14:0/22:2、Cer AP d14:0/22:2、HexCer AP t18:0/16:0等8种神经酰胺与低温胁迫关系更为密切。说明神经酰胺是甜椒低温胁迫下的关键代谢产物。8种关键神经酰胺含量与相对电导率的相关性分析表明，Cer ADS d17:0/19:0可作为预测甜椒冷害发生的初步生物标志物。研究结果为进一步研究甜椒低温冷害机理和探索相应的防治技术提供了线索和基础。

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