Postharvest Biology and Technology最新文献_第4页

Multi-targeted antifungal mechanism of punicalagin against Botrytis cinerea and its role in preserving strawberry quality 甘薯苷对葡萄灰霉病的多靶点抗真菌机理及其对草莓品质的保护作用

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2026-01-19 DOI: 10.1016/j.postharvbio.2026.114156

Muhammad Nawaz , Junkun Pan , Hui Liu , Keying Feng , Jiechao Liu , Wenbo Yang , Zhenzhen Lv , Qiang Zhang , Zhonggao Jiao

Gray mold, caused by the fungus Botrytis cinerea, is a key postharvest disease of strawberries, resulting in substantial fruit rot and economic losses. This study elucidated the comprehensive antifungal mechanism of punicalagin against B. cinerea. The antifungal action of punicalagin primarily involved the disruption of fungal cell membrane integrity, as evidenced by the induction of cytoplasmic leakage, accumulation of malondialdehyde and reduction in ergosterol and trehalose. Ultrastructural analysis revealed severe hyphal damage after punicalagin treatment. Punicalagin also induced apoptosis through mitochondrial membrane dysfunction and the accumulation of reactive oxygen species (ROS). Furthermore, transcriptomic analysis identified 2589 downregulated and 1157 upregulated genes in punicalagin-treated mycelia, with a particular impact on membrane lipid and carbohydrate metabolism pathways. These transcriptomic alterations, representing impaired energy production and antioxidant systems, were further validated by corresponding metabolomic analysis. Additionally, punicalagin treatment preserved the quality of strawberry fruit by improving cell wall structure to maintain firmness and augmenting phenolic content, potentially enhancing resistance. These multifaceted mechanisms collectively contributed to the exceptional efficacy of punicalagin in controlling B. cinerea. These findings suggest that punicalagin could be a promising natural antifungal alternative, offering effective control of gray mold and maintaining the quality of strawberries in storage.

灰霉病是草莓采后主要病害，由葡萄灰霉病（Botrytis cinerea）引起，造成大量果实腐烂和经济损失。本研究阐明了槟榔苷对灰霉病菌的综合抑菌机制。punicalagin的抗真菌作用主要涉及破坏真菌细胞膜的完整性，这可以通过诱导细胞质渗漏、丙二醛积累和麦角甾醇和海藻糖减少来证明。超微结构分析显示，针刺后菌丝损伤严重。Punicalagin还通过线粒体膜功能障碍和活性氧（ROS）的积累诱导细胞凋亡。此外，转录组学分析发现，在槟榔素处理的菌丝体中，有2589个下调基因和1157个上调基因，对膜脂和碳水化合物代谢途径有特别的影响。这些转录组改变，代表了能量产生和抗氧化系统的受损，通过相应的代谢组学分析进一步验证。此外，槟榔苷处理通过改善细胞壁结构以保持果实硬度和增加酚类含量来保持草莓果实的品质，从而有可能增强抗性。这些多方面的机制共同促成了槟榔苷在控制葡萄球菌方面的特殊功效。这些结果表明，槟榔苷可能是一种有前景的天然抗真菌替代品，可以有效地控制草莓的灰霉病，并保持草莓的储存质量。

{"title":"Multi-targeted antifungal mechanism of punicalagin against Botrytis cinerea and its role in preserving strawberry quality","authors":"Muhammad Nawaz , Junkun Pan , Hui Liu , Keying Feng , Jiechao Liu , Wenbo Yang , Zhenzhen Lv , Qiang Zhang , Zhonggao Jiao","doi":"10.1016/j.postharvbio.2026.114156","DOIUrl":"10.1016/j.postharvbio.2026.114156","url":null,"abstract":"<div><div>Gray mold, caused by the fungus <em>Botrytis cinerea</em>, is a key postharvest disease of strawberries, resulting in substantial fruit rot and economic losses. This study elucidated the comprehensive antifungal mechanism of punicalagin against <em>B. cinerea</em>. The antifungal action of punicalagin primarily involved the disruption of fungal cell membrane integrity, as evidenced by the induction of cytoplasmic leakage, accumulation of malondialdehyde and reduction in ergosterol and trehalose. Ultrastructural analysis revealed severe hyphal damage after punicalagin treatment. Punicalagin also induced apoptosis through mitochondrial membrane dysfunction and the accumulation of reactive oxygen species (ROS). Furthermore, transcriptomic analysis identified 2589 downregulated and 1157 upregulated genes in punicalagin-treated mycelia, with a particular impact on membrane lipid and carbohydrate metabolism pathways. These transcriptomic alterations, representing impaired energy production and antioxidant systems, were further validated by corresponding metabolomic analysis. Additionally, punicalagin treatment preserved the quality of strawberry fruit by improving cell wall structure to maintain firmness and augmenting phenolic content, potentially enhancing resistance. These multifaceted mechanisms collectively contributed to the exceptional efficacy of punicalagin in controlling <em>B. cinerea</em>. These findings suggest that punicalagin could be a promising natural antifungal alternative, offering effective control of gray mold and maintaining the quality of strawberries in storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114156"},"PeriodicalIF":6.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated volatilomics and transcriptomics reveal the aroma formation mechanism in Actinidia arguta during postharvest ripening 综合挥发物学和转录组学研究揭示了软枣猕猴桃采后成熟过程中香气形成机制

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-12-10 DOI: 10.1016/j.postharvbio.2025.114118

Heran Xu, Guanlin Qian, Miao Yan, Huanyu Wang, Naizhu Liu, Bin Li, Weiyu Liu, Guang Xin, Song Pan

Aroma is a critical quality attribute determining consumer acceptance of Actinidia arguta. However, the molecular mechanisms underlying aroma formation during postharvest ripening remain poorly understood. This study employed integrated volatilomics and transcriptomics approaches to elucidate the dynamic changes in aroma-active compounds and their biosynthetic regulation of A. arguta ‘Changjiang No. 1’ stored at 20 °C for 12 days. A total of 51 volatile organic compounds were identified, with dramatic compositional shifts from C6/C9 aldehydes (trans-2-hexenal, (2E,6Z)-nona-2,6-dienal) to fruity-floral terpenoids (β-ionone, β-damascenone) and esters during ripening. The odor activity value (OAV) analysis revealed a significant aroma transformation during fruit ripening. This process involved a substantial decline in green aroma aldehydes, notably (2E,6Z)-nona-2,6-dienal (OAV decreasing from 415 to undetectable), coupled with a prominent increase in fruity-floral terpenoids. β-Ionone became the dominant aroma contributor, with its OAV increasing from 8.3 to 1485.71, complemented by β-damascenone (OAV = 733.33). Transcriptome analysis identified differentially expressed genes significantly enriched in fatty acid metabolism, terpenoid biosynthesis, and phenylpropanoid pathways. Weighted gene co-expression network analysis identified two key modules: MEturquoise (associated with green aroma biosynthesis) and MEbrown (associated with floral-fruity aroma formation). Hub gene analysis revealed AaLOX, AaHPL, and AaAAT as key regulators of green volatiles, while AaHMGR, AaMDS, AaHDR and AaGPPS emerged as central to terpenoid biosynthesis. These findings provide comprehensive insights into the molecular regulation of aroma development in A. arguta and establish a foundation for targeted postharvest quality management strategies.

香气是决定消费者接受猕猴桃的关键品质属性。然而，在采后成熟过程中香气形成的分子机制仍然知之甚少。本研究采用挥发性和转录组学相结合的方法，研究了香菇长江1号在20℃贮藏12 d后香气活性物质的动态变化及其生物合成调控。共鉴定出51种挥发性有机化合物，其组成在成熟过程中从C6/C9醛（反式2-己烯醛，(2E,6Z)-nona-2,6-二烯醛）转变为果花萜类（β-ionone, β-damascenone）和酯类。气味活性值（OAV）分析表明，果实在成熟过程中发生了显著的香气转化。这一过程涉及绿色香气醛的大幅下降，特别是(2E,6Z)-nona-2,6-二烯醛（OAV从415下降到无法检测到），伴随着果花香萜类化合物的显著增加。β-碘酮是主要的香气贡献者，其OAV从8.3增加到1485.71，辅之以β-大马士革酮（OAV = 733.33）。转录组分析发现了脂肪酸代谢、萜类生物合成和苯丙类途径中显著富集的差异表达基因。加权基因共表达网络分析确定了两个关键模块：MEturquoise（与绿色香气生物合成有关）和MEbrown（与花果香气形成有关）。Hub基因分析显示，AaLOX、AaHPL和AaAAT是绿色挥发物的关键调控因子，而AaHMGR、AaMDS、AaHDR和AaGPPS是萜类生物合成的关键调控因子。这些研究结果为研究香豆香气发育的分子调控提供了全面的见解，并为有针对性的采后质量管理策略奠定了基础。

{"title":"Integrated volatilomics and transcriptomics reveal the aroma formation mechanism in Actinidia arguta during postharvest ripening","authors":"Heran Xu, Guanlin Qian, Miao Yan, Huanyu Wang, Naizhu Liu, Bin Li, Weiyu Liu, Guang Xin, Song Pan","doi":"10.1016/j.postharvbio.2025.114118","DOIUrl":"10.1016/j.postharvbio.2025.114118","url":null,"abstract":"<div><div>Aroma is a critical quality attribute determining consumer acceptance of <em>Actinidia arguta</em>. However, the molecular mechanisms underlying aroma formation during postharvest ripening remain poorly understood. This study employed integrated volatilomics and transcriptomics approaches to elucidate the dynamic changes in aroma-active compounds and their biosynthetic regulation of <em>A. arguta</em> ‘Changjiang No. 1’ stored at 20 °C for 12 days. A total of 51 volatile organic compounds were identified, with dramatic compositional shifts from C6/C9 aldehydes (<em>trans</em>-2-hexenal, (2<em>E</em>,6<em>Z</em>)-nona-2,6-dienal) to fruity-floral terpenoids (<em>β</em>-ionone, <em>β</em>-damascenone) and esters during ripening. The odor activity value (OAV) analysis revealed a significant aroma transformation during fruit ripening. This process involved a substantial decline in green aroma aldehydes, notably (2<em>E</em>,6<em>Z</em>)-nona-2,6-dienal (OAV decreasing from 415 to undetectable), coupled with a prominent increase in fruity-floral terpenoids. <em>β</em>-Ionone became the dominant aroma contributor, with its OAV increasing from 8.3 to 1485.71, complemented by <em>β</em>-damascenone (OAV = 733.33). Transcriptome analysis identified differentially expressed genes significantly enriched in fatty acid metabolism, terpenoid biosynthesis, and phenylpropanoid pathways. Weighted gene co-expression network analysis identified two key modules: MEturquoise (associated with green aroma biosynthesis) and MEbrown (associated with floral-fruity aroma formation). Hub gene analysis revealed <em>AaLOX</em>, <em>AaHPL</em>, and <em>AaAAT</em> as key regulators of green volatiles, while <em>AaHMGR</em>, <em>AaMDS</em>, <em>AaHDR</em> and <em>AaGPPS</em> emerged as central to terpenoid biosynthesis. These findings provide comprehensive insights into the molecular regulation of aroma development in <em>A. arguta</em> and establish a foundation for targeted postharvest quality management strategies.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114118"},"PeriodicalIF":6.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145737248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chlorine dioxide fumigation induces transcriptomic and metabolomic reprogramming enhancing resistance to soft rot in kiwifruit 二氧化氯熏蒸诱导转录组和代谢组重编程，增强猕猴桃对软腐病的抗性

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-12-10 DOI: 10.1016/j.postharvbio.2025.114117

Ang Li , Shuang Luo , Di Feng , Xiaoya Chen , Zhidan Zhao , Tiantian Li , Yunhe Meng , Xiaoqin Yang , Yunjiang Cheng , Yunliu Zeng

Soft rot is a major cause of postharvest decay in kiwifruit. Chlorine dioxide (ClO₂) has been widely applied as a preservative in many fruit and vegetables, but its efficacy and underlying mechanisms against soft rot in kiwifruit are largely unknown. In this study, ClO₂ fumigant was applied to suppress two primary soft rot fungal pathogens, Botryosphaeria dothidea and Diaporthe eres in ‘Yunhai No. 1’ and ‘Hongyang’ kiwifruit (Actinidia chinensis). Treatment with 400 mg ClO₂ reduced disease incidence by up to 93 % and lesion diameter by 22.5 mm. Integrated transcriptome-metabolome analyses were performed to investigate its effect on the induction of plant defense responses. Transcriptomic analysis revealed 2828 differentially expressed genes, highlighting enrichment in defense-related pathways and upregulation of ERF, MYB, and WRKY transcription factors. Metabolomic profiling identified 1172 metabolites, with strong accumulation of antifungal compounds like jasmonic acid and luteolin-7-O-glucuronide under ClO₂ treatment. Correlation analysis confirmed strong associations between ERF transcription factors and key antimicrobial metabolites. Collectively, these findings demonstrate that ClO₂ fumigation controls soft rot not only through direct antifungal action but also by inducing host defense mechanisms, thereby providing new insights and practical references for eco-friendly preservation of kiwifruit.

软腐病是猕猴桃采后腐烂的主要原因。二氧化氯（ClO2）作为防腐剂被广泛应用于许多水果和蔬菜中，但其防治猕猴桃软腐病的功效和潜在机制在很大程度上是未知的。本研究用ClO2熏蒸剂对云海1号和红阳猕猴桃中的两种软腐真菌病原菌Botryosphaeria dothidea和Diaporthe eres进行了抑制。400 mg ClO₂治疗可使疾病发生率降低93 %，病变直径增加22.5 mm。通过综合转录组-代谢组分析来研究其对植物防御反应的诱导作用。转录组学分析显示2828个差异表达基因，突出显示防御相关通路的富集和ERF、MYB和WRKY转录因子的上调。代谢组学分析鉴定出1172种代谢物，在clo2处理下，茉莉酸和木犀草素-7- o -葡萄糖醛酸等抗真菌化合物大量积累。相关分析证实了ERF转录因子与关键抗菌代谢物之间的强相关性。综上，这些研究结果表明，ClO₂熏蒸控制软腐病不仅通过直接的抗真菌作用，还通过诱导宿主防御机制，从而为猕猴桃的生态保鲜提供了新的见解和实用参考。

{"title":"Chlorine dioxide fumigation induces transcriptomic and metabolomic reprogramming enhancing resistance to soft rot in kiwifruit","authors":"Ang Li , Shuang Luo , Di Feng , Xiaoya Chen , Zhidan Zhao , Tiantian Li , Yunhe Meng , Xiaoqin Yang , Yunjiang Cheng , Yunliu Zeng","doi":"10.1016/j.postharvbio.2025.114117","DOIUrl":"10.1016/j.postharvbio.2025.114117","url":null,"abstract":"<div><div>Soft rot is a major cause of postharvest decay in kiwifruit. Chlorine dioxide (ClO<sub>2</sub>) has been widely applied as a preservative in many fruit and vegetables, but its efficacy and underlying mechanisms against soft rot in kiwifruit are largely unknown. In this study, ClO<sub>2</sub> fumigant was applied to suppress two primary soft rot fungal pathogens, <em>Botryosphaeria dothidea</em> and <em>Diaporthe eres</em> in ‘Yunhai No. 1’ and ‘Hongyang’ kiwifruit (<em>Actinidia chinensis</em>). Treatment with 400 mg ClO₂ reduced disease incidence by up to 93 % and lesion diameter by 22.5 mm. Integrated transcriptome-metabolome analyses were performed to investigate its effect on the induction of plant defense responses. Transcriptomic analysis revealed 2828 differentially expressed genes, highlighting enrichment in defense-related pathways and upregulation of ERF, MYB, and WRKY transcription factors. Metabolomic profiling identified 1172 metabolites, with strong accumulation of antifungal compounds like jasmonic acid and luteolin-7-O-glucuronide under ClO₂ treatment. Correlation analysis confirmed strong associations between ERF transcription factors and key antimicrobial metabolites. Collectively, these findings demonstrate that ClO₂ fumigation controls soft rot not only through direct antifungal action but also by inducing host defense mechanisms, thereby providing new insights and practical references for eco-friendly preservation of kiwifruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114117"},"PeriodicalIF":6.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145737319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-destructive monitoring of postharvest quality changes in chili (Capsicum frutescens) during storage using image processing coupled with machine learning 使用图像处理与机器学习相结合的方法对辣椒（Capsicum frutescens）在储存期间的采后质量变化进行无损监测

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-12-22 DOI: 10.1016/j.postharvbio.2025.114140

Wong Junyang , Mohamad Yusof Maskat , Maimunah Mohd Ali

Chili (Capsicum frutescens) is an important vegetable crop widely cultivated in tropical and subtropical regions, where postharvest quality deterioration particularly color and freshness loss, poses significant challenges during storage. Traditional visual inspection methods are often subjective and inconsistent. This study evaluated the postharvest quality of bird’s eye chilies stored at 5 °C and 28 °C on Days 1, 3, and 5 using non-destructive image processing combined with machine learning approach. Image features were extracted from chili samples and classified using Linear Discriminant Analysis (LDA), Support Vector Machine (SVM), k-Nearest Neighbors (k-NN), and ensemble model. Using a 70:30 training–testing split, SVM achieved the highest average classification accuracy (69.1 % at 5 °C and 66.7 % at 28 °C). Under 10-fold stratified cross-validation, k-NN and ensemble models demonstrated improved robustness, with the ensemble yielding the highest average classification accuracy (74.08 % at 5 °C and 79.26 % at 28 °C). To complement the machine learning models, deep learning models where a feature-level 1D-Convolutional Neural Network was implemented, achieving an average 57.6 % accuracy under 10-fold, while an image-based MobileNetV2 architecture achieved 78.8 % validation accuracy. These results highlighted that automatic feature extraction can outperform hand-crafted features for postharvest chili quality assessment. Additionally, Partial Least Squares (PLS) regression identified the L* value as the most effective color parameter for quality detection (R² > 0.93). Overall, integrating image processing with machine learning and supported by deep learning offers a reliable, non-invasive framework for evaluating postharvest chili quality.

辣椒（Capsicum frutescens）是一种广泛种植于热带和亚热带地区的重要蔬菜作物，其采后品质劣化，尤其是色泽和新鲜度的丧失，给贮藏带来了重大挑战。传统的目视检查方法往往是主观的和不一致的。本研究利用非破坏性图像处理结合机器学习方法，评估了在5°C和28°C条件下储存的鸟眼辣椒在第1、3和5天的采后质量。利用线性判别分析（LDA）、支持向量机（SVM）、k-最近邻（k-NN）和集成模型对辣椒样本的图像特征进行分类。使用70:30的训练-测试分割，SVM获得了最高的平均分类准确率（5°C时为69.1 %，28°C时为66.7 %）。在10倍分层交叉验证下，k-NN和集成模型显示出更好的鲁棒性，其中集成的平均分类准确率最高（5°C时为74.08 %，28°C时为79.26 %）。为了补充机器学习模型，实现了特征级1d -卷积神经网络的深度学习模型，在10倍下实现了平均57.6 %的准确率，而基于图像的MobileNetV2架构实现了78.8 %的验证准确率。这些结果表明，在采后辣椒品质评估中，自动特征提取优于手工特征提取。此外，偏最小二乘（PLS）回归发现L*值是质量检测最有效的颜色参数（R²> 0.93）。总的来说，将图像处理与机器学习结合起来，并在深度学习的支持下，为评估采后辣椒的质量提供了一个可靠的、非侵入性的框架。

{"title":"Non-destructive monitoring of postharvest quality changes in chili (Capsicum frutescens) during storage using image processing coupled with machine learning","authors":"Wong Junyang , Mohamad Yusof Maskat , Maimunah Mohd Ali","doi":"10.1016/j.postharvbio.2025.114140","DOIUrl":"10.1016/j.postharvbio.2025.114140","url":null,"abstract":"<div><div>Chili (<em>Capsicum frutescens</em>) is an important vegetable crop widely cultivated in tropical and subtropical regions, where postharvest quality deterioration particularly color and freshness loss, poses significant challenges during storage. Traditional visual inspection methods are often subjective and inconsistent. This study evaluated the postharvest quality of bird’s eye chilies stored at 5 °C and 28 °C on Days 1, 3, and 5 using non-destructive image processing combined with machine learning approach. Image features were extracted from chili samples and classified using Linear Discriminant Analysis (LDA), Support Vector Machine (SVM), k-Nearest Neighbors (k-NN), and ensemble model. Using a 70:30 training–testing split, SVM achieved the highest average classification accuracy (69.1 % at 5 °C and 66.7 % at 28 °C). Under 10-fold stratified cross-validation, k-NN and ensemble models demonstrated improved robustness, with the ensemble yielding the highest average classification accuracy (74.08 % at 5 °C and 79.26 % at 28 °C). To complement the machine learning models, deep learning models where a feature-level 1D-Convolutional Neural Network was implemented, achieving an average 57.6 % accuracy under 10-fold, while an image-based MobileNetV2 architecture achieved 78.8 % validation accuracy. These results highlighted that automatic feature extraction can outperform hand-crafted features for postharvest chili quality assessment. Additionally, Partial Least Squares (PLS) regression identified the L* value as the most effective color parameter for quality detection (R² > 0.93). Overall, integrating image processing with machine learning and supported by deep learning offers a reliable, non-invasive framework for evaluating postharvest chili quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114140"},"PeriodicalIF":6.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sound treatment accelerates banana peel degreening via calcium-signaling-mediated ethylene action 声音处理通过钙信号介导的乙烯作用加速香蕉皮脱脂

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-12-15 DOI: 10.1016/j.postharvbio.2025.114121

Shuhan Yang , Jiayi Liu , Yicheng Ren , Dong Li , Zisheng Luo , Yanpei Chen

Sound treatment (ST) is a non-invasive and eco-friendly technology with potential in postharvest fruit quality regulation. However, its effects and underlying mechanisms in postharvest climacteric fruits remain an unexplored frontier. This study evaluated the effects of ST (1 kHz, 100 dB, 12 h/d) on the postharvest ripening of bananas (Musa acuminata cv. Huangjinjiao, AAA) under ethephon-based ethylene fumigation. ST significantly accelerated peel degreening, with chlorophyll content in the control group 2.3 times higher than that in the ST group by 8 d. In addition, the firmness in the ST group was 57.9 % (peel) and 10.3 % (flesh) of the value in the CT group on 8 d, reflecting an accelerated softening process. Under ST, the soluble calcium level in the ST group was 1.58 times that in the CT group, and the expression of genes related to calcium and ethylene signaling was upregulated. Moreover, the activities of ethylene biosynthesis enzymes were enhanced, thereby promoting ethylene production. Consequently, chlorophyll-degrading enzymes were activated, accelerating peel degreening. Overall, this study provides the first systematic evidence of how ST modulates calcium–ethylene crosstalk to regulate fruit quality during ripening and highlights its potential as a green strategy for improving postharvest management and commercial handling of bananas.

声音处理技术是一种无创、环保的水果采后品质调控技术。然而，其在采后更年期果实中的作用和潜在机制仍然是一个未探索的前沿。本研究评价了ST（1 kHz, 100 dB, 12 h/d）对香蕉采后成熟的影响。黄金角，AAA)乙烯基乙烯熏蒸。ST显著加速了果皮去角质，8 d时，对照组的叶绿素含量是ST组的2.3倍。另外，8 d时，ST组果皮的硬度为CT组的57.9 %（果皮），10.3 %（果肉），软化过程加快。在ST作用下，ST组的可溶性钙水平是CT组的1.58倍，钙和乙烯信号相关基因表达上调。此外，乙烯生物合成酶活性增强，从而促进乙烯的生产。因此，叶绿素降解酶被激活，加速果皮去角质。总的来说，这项研究提供了ST如何调节钙-乙烯串扰来调节成熟过程中的水果质量的第一个系统证据，并强调了其作为改善香蕉采后管理和商业处理的绿色策略的潜力。

{"title":"Sound treatment accelerates banana peel degreening via calcium-signaling-mediated ethylene action","authors":"Shuhan Yang , Jiayi Liu , Yicheng Ren , Dong Li , Zisheng Luo , Yanpei Chen","doi":"10.1016/j.postharvbio.2025.114121","DOIUrl":"10.1016/j.postharvbio.2025.114121","url":null,"abstract":"<div><div>Sound treatment (ST) is a non-invasive and eco-friendly technology with potential in postharvest fruit quality regulation. However, its effects and underlying mechanisms in postharvest climacteric fruits remain an unexplored frontier. This study evaluated the effects of ST (1 kHz, 100 dB, 12 h/d) on the postharvest ripening of bananas (<em>Musa acuminata</em> cv. Huangjinjiao, AAA) under ethephon-based ethylene fumigation. ST significantly accelerated peel degreening, with chlorophyll content in the control group 2.3 times higher than that in the ST group by 8 d. In addition, the firmness in the ST group was 57.9 % (peel) and 10.3 % (flesh) of the value in the CT group on 8 d, reflecting an accelerated softening process. Under ST, the soluble calcium level in the ST group was 1.58 times that in the CT group, and the expression of genes related to calcium and ethylene signaling was upregulated. Moreover, the activities of ethylene biosynthesis enzymes were enhanced, thereby promoting ethylene production. Consequently, chlorophyll-degrading enzymes were activated, accelerating peel degreening. Overall, this study provides the first systematic evidence of how ST modulates calcium–ethylene crosstalk to regulate fruit quality during ripening and highlights its potential as a green strategy for improving postharvest management and commercial handling of bananas.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114121"},"PeriodicalIF":6.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A hidden alliance: The potential role of bacteria in the virulence of postharvest fungal pathogens 一个隐藏的联盟：细菌在收获后真菌病原体毒力中的潜在作用

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-12-16 DOI: 10.1016/j.postharvbio.2025.114130

Samir Droby , V. Yeka Zhimo , Vijay Kumar Sharma , Rotem Bartuv , Michael Wisniewski , Hongyin Zhang , Shiri Freilich , Davide Spadaro

Postharvest losses caused by fungal pathogens are traditionally explained through a single-pathogen paradigm, where disease results from pathogen pathogenicity and virulence factors acting on a susceptible host under favorable conditions. However, emerging evidence challenges this view, pointing instead to the role of microbial consortia - the postharvest pathobiome - in shaping infection outcomes. In this opinion article, we explore the potential hidden alliance between fungi and bacteria that enhances the virulence of postharvest pathogens. Using apple - P. expansum as a model system, preliminary experiments revealed that co-inoculation with certain bacterial isolates significantly increased lesion development compared to the fungus alone, whereas bacteria alone were non-pathogenic. Amplicon sequencing further showed that fungal infection altered the wound microbiome, favoring the proliferation of anaerobic and facultative anaerobic bacteria. We discuss multiple mechanisms by which bacteria may contribute to fungal virulence, including nutrient cross-feeding, biofilm formation, chemical signaling, pH modulation, alteration of host defenses, and the formation of endosymbiotic alliances. Recognition of this fungal-bacterial synergy has profound implications for postharvest management, as fungicide or biocontrol-based strategies may overlook the supportive role of bacteria and inadvertently favor more resilient pathogenic consortia. We propose that future disease control strategies adopt a microbiome-driven perspective, integrating high-throughput sequencing, metabolomics, imaging, and in silico modeling to disentangle these interactions and guide the development of sustainable, biologically informed interventions. Understanding and manipulating such inter-kingdom alliances may represent a crucial step toward reducing postharvest decay and improving global food security.

由真菌病原体引起的采后损失传统上通过单一病原体范式来解释，其中疾病是由病原体致病性和毒力因素在有利条件下作用于易感宿主引起的。然而，新出现的证据挑战了这一观点，而是指出了微生物群落-采收后病原体组-在塑造感染结果中的作用。在这篇观点文章中，我们探讨了真菌和细菌之间潜在的隐藏联盟，增强了采后病原体的毒力。以苹果-膨松菌为模型系统，初步实验发现，与单独接种真菌相比，与某些细菌分离株共接种显著增加了病变的发展，而单独接种细菌则无致病性。扩增子测序进一步表明，真菌感染改变了伤口微生物群，有利于厌氧和兼性厌氧细菌的增殖。我们讨论了细菌可能对真菌毒力起作用的多种机制，包括营养交叉摄食、生物膜形成、化学信号传导、pH调节、宿主防御的改变以及内共生联盟的形成。认识到这种真菌-细菌协同作用对采后管理具有深远的意义，因为杀菌剂或基于生物防治的策略可能忽略了细菌的支持作用，无意中有利于更具弹性的致病菌群。我们建议未来的疾病控制策略采用微生物组驱动的视角，整合高通量测序、代谢组学、成像和计算机建模，以理清这些相互作用，并指导可持续的、生物学知情的干预措施的发展。了解和操纵这种王国间联盟可能是减少采后腐烂和改善全球粮食安全的关键一步。

{"title":"A hidden alliance: The potential role of bacteria in the virulence of postharvest fungal pathogens","authors":"Samir Droby , V. Yeka Zhimo , Vijay Kumar Sharma , Rotem Bartuv , Michael Wisniewski , Hongyin Zhang , Shiri Freilich , Davide Spadaro","doi":"10.1016/j.postharvbio.2025.114130","DOIUrl":"10.1016/j.postharvbio.2025.114130","url":null,"abstract":"<div><div>Postharvest losses caused by fungal pathogens are traditionally explained through a single-pathogen paradigm, where disease results from pathogen pathogenicity and virulence factors acting on a susceptible host under favorable conditions. However, emerging evidence challenges this view, pointing instead to the role of microbial consortia - the postharvest pathobiome - in shaping infection outcomes. In this opinion article, we explore the potential hidden alliance between fungi and bacteria that enhances the virulence of postharvest pathogens. Using apple - <em>P. expansum</em> as a model system, preliminary experiments revealed that co-inoculation with certain bacterial isolates significantly increased lesion development compared to the fungus alone, whereas bacteria alone were non-pathogenic. Amplicon sequencing further showed that fungal infection altered the wound microbiome, favoring the proliferation of anaerobic and facultative anaerobic bacteria. We discuss multiple mechanisms by which bacteria may contribute to fungal virulence, including nutrient cross-feeding, biofilm formation, chemical signaling, pH modulation, alteration of host defenses, and the formation of endosymbiotic alliances. Recognition of this fungal-bacterial synergy has profound implications for postharvest management, as fungicide or biocontrol-based strategies may overlook the supportive role of bacteria and inadvertently favor more resilient pathogenic consortia. We propose that future disease control strategies adopt a microbiome-driven perspective, integrating high-throughput sequencing, metabolomics, imaging, and <em>in silico</em> modeling to disentangle these interactions and guide the development of sustainable, biologically informed interventions. Understanding and manipulating such inter-kingdom alliances may represent a crucial step toward reducing postharvest decay and improving global food security.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114130"},"PeriodicalIF":6.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprotective potential of Bacillus spp. against postharvest pathogens of Khasi Mandarin (Citrus reticulata Blanco) with enhanced shelf life 芽孢杆菌对延长保质期的卡西柑采后病原菌的生物保护潜力

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-12-24 DOI: 10.1016/j.postharvbio.2025.114142

Trishna Taye , Popy Bora

The global citrus industry faces significant annual economic losses due to postharvest decay pathogens. This study focused on identifying the major postharvest pathogens affecting Khasi Mandarin (Citrus reticulata Blanco) and evaluating the efficacy of Bacillus spp. as biocontrol agents, as well as their impact on fruit quality. Based on morpho-cultural characteristics, the isolated pathogens were identified as Penicillium digitatum (PQ606361) and Aspergillus niger (PV657140). Four Bacillus spp. Cell-free supernatants (CFS) were screened against these pathogens, all of which significantly inhibited fungal mycelial growth. Among them, Bacillus subtilis LB22 CFS exhibited the highest inhibition, at 74.60 % for P. digitatum and 86.20 % for A. niger. Scanning Electron Microscopy (SEM) revealed morphological disintegration in fungal hyphae and spores upon treatment with B. subtilis LB22 CFS. GC-MS analysis of the LB22 CFS identified nine antimicrobial and four ethylene-inhibiting compounds, including 4-di-tert-butylphenol, n-hexadecanoic acid, and 1H-indene derivatives. In-vivo assays of CFS demonstrated that fruit coating with the B. subtilis CFS prevented A. niger and P. digitatum invasion in khasi mandarin fruits with 100 % bioprotective efficiency upto 15 days against 100 % fruit decay in control at 3 days. Quality assessment of treated fruits after 15 days of storage revealed a total soluble solids (TSS) value of 11.90°Brix and titratable acidity of 0.85 %, both statistically non-significant compared to the control. Organoleptic evaluations, based on colour, flavour, texture, taste, and overall acceptability, supported the efficacy of the treatment. Collectively, our findings confirm that B. subtilis LB22 acts as a potent biocontrol agent against green and black mold in both in vitro and in vivo conditions. Our study hence perch Bacillus subtilis LB22 CFS based fruit coating as a promising option for post harvest handling of Khasi mandarin fruits, preventing fruit decay and enhancing the shelf life.

由于采后腐烂病原体，全球柑橘产业面临着重大的年度经济损失。本研究旨在鉴定影响卡西柑采后的主要病原菌，并评价芽孢杆菌（Bacillus spp.）作为生物防治剂的效果及其对果实品质的影响。根据形态培养特征，分离的病原菌鉴定为指状青霉（PQ606361）和黑曲霉（PV657140）。筛选出4种芽孢杆菌无细胞上清液（CFS），均能显著抑制真菌菌丝生长。其中，枯草芽孢杆菌LB22 CFS对digitatum和niger的抑制率分别为74.60 %和86.20 %。扫描电镜（SEM）显示枯草芽孢杆菌LB22 CFS对真菌菌丝和孢子的形态学破坏。GC-MS分析鉴定出9种抗菌化合物和4种乙烯抑制化合物，包括4-二叔丁基酚、正十六酸和1h -独立衍生物。CFS的体内试验表明，用枯草芽孢杆菌CFS包衣可有效防止黑曲霉和指状线虫对卡西柑果实的侵袭，15天内的生物保护效率为100% %，3天内的果实腐烂率为100% %。贮藏15天后的果实质量评价显示，总可溶性固形物（TSS）值为11.90°Brix，可滴定酸度为0.85 %，与对照相比均无统计学意义。基于颜色、风味、质地、味道和总体可接受性的感官评价支持了治疗的有效性。总之，我们的研究结果证实，枯草芽孢杆菌LB22在体外和体内条件下都是一种有效的生物防治剂，可以对抗绿霉和黑霉。本研究认为，枯草芽孢杆菌LB22 CFS为基材的覆膜剂是一种很有前途的覆膜剂，可用于卡西柑采后处理，防止果实腐烂，延长果实保质期。

{"title":"Bioprotective potential of Bacillus spp. against postharvest pathogens of Khasi Mandarin (Citrus reticulata Blanco) with enhanced shelf life","authors":"Trishna Taye , Popy Bora","doi":"10.1016/j.postharvbio.2025.114142","DOIUrl":"10.1016/j.postharvbio.2025.114142","url":null,"abstract":"<div><div>The global citrus industry faces significant annual economic losses due to postharvest decay pathogens. This study focused on identifying the major postharvest pathogens affecting Khasi Mandarin (<em>Citrus reticulata</em> Blanco) and evaluating the efficacy of <em>Bacillus</em> spp. as biocontrol agents, as well as their impact on fruit quality. Based on morpho-cultural characteristics, the isolated pathogens were identified as <em>Penicillium digitatum</em> (PQ606361) and <em>Aspergillus niger</em> (PV657140). Four <em>Bacillus</em> spp. Cell-free supernatants (CFS) were screened against these pathogens, all of which significantly inhibited fungal mycelial growth. Among them, <em>Bacillus subtilis</em> LB22 CFS exhibited the highest inhibition, at 74.60 % for <em>P. digitatum</em> and 86.20 % for <em>A. niger</em>. Scanning Electron Microscopy (SEM) revealed morphological disintegration in fungal hyphae and spores upon treatment with <em>B. subtilis</em> LB22 CFS. GC-MS analysis of the LB22 CFS identified nine antimicrobial and four ethylene-inhibiting compounds, including 4-di-tert-butylphenol, <em>n</em>-hexadecanoic acid, and 1H-indene derivatives. <em>In-vivo</em> assays of CFS demonstrated that fruit coating with the <em>B. subtilis</em> CFS prevented <em>A. niger</em> and <em>P. digitatum</em> invasion in khasi mandarin fruits with 100 % bioprotective efficiency upto 15 days against 100 % fruit decay in control at 3 days. Quality assessment of treated fruits after 15 days of storage revealed a total soluble solids (TSS) value of 11.90°Brix and titratable acidity of 0.85 %, both statistically non-significant compared to the control. Organoleptic evaluations, based on colour, flavour, texture, taste, and overall acceptability, supported the efficacy of the treatment. Collectively, our findings confirm that <em>B. subtilis</em> LB22 acts as a potent biocontrol agent against green and black mold in both in vitro and <em>in vivo</em> conditions. Our study hence perch <em>Bacillus subtilis</em> LB22 CFS based fruit coating as a promising option for post harvest handling of Khasi mandarin fruits, preventing fruit decay and enhancing the shelf life.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114142"},"PeriodicalIF":6.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PpMYC2 positively regulates chilling tolerance of peach fruit by suppressing sucrose degradation PpMYC2通过抑制蔗糖降解正向调节桃果的抗寒性

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2026-01-19 DOI: 10.1016/j.postharvbio.2026.114179

Qian Li , Yingying Wei , Yi Chen , Shu Jiang , Jianfen Ye , Fangming Wu , Feng Xu , Xingfeng Shao

MYC2 transcription factor has been extensively investigated in plant stress responses. However, its role in regulating fruit cold tolerance remains largely unexplored. In this study, exposure to cold stress rapidly activated the transcription of PpMYC2 in peach fruit. DNA affinity purification sequencing (DAP-seq) data revealed that PpMYC2 showed high affinity for the promoter region of PpVIN2 - a key gene encoding acidic vacuolar invertase (VIN) critical for sucrose catabolism in peach fruit. Yeast one-hybridization (Y1H), electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter (DLR) assay collectively revealed that PpMYC2 directly bound to the G-box cis-element in the PpVIN2 promoter and significantly suppressed its promoter activity. Transient overexpression of PpMYC2 in peach fruit resulted in downregulated transcription of PpVIN2, reduced VIN activity, and increased sucrose content, whereas opposite changes were detected in peach fruit with transient silencing of PpMYC2. Additionally, we generated tomato plants stably overexpressing PpMYC2 using Agrobacterium-mediated transformation, and observed that PpMYC2-overexpressing tomato fruit exhibited significantly enhanced cold tolerance, accompanied by the inhibition of sucrose degradation. Collectively, these discoveries indicate that PpMYC2 acts as a transcriptional repressor of PpVIN2 under cold stress, thereby reducing sucrose breakdown and enhancing peach fruit chilling tolerance. This study identifies PpMYC2 as a potential molecular target for alleviating chilling injury in peach fruit.

MYC2转录因子在植物逆境应答中被广泛研究。然而，它在调节水果耐寒性方面的作用仍未得到充分研究。在本研究中，暴露于冷胁迫下的桃果快速激活PpMYC2的转录。DNA亲和纯化测序（DAP-seq）数据显示，PpMYC2对PpVIN2的启动子区域具有高亲和性，PpVIN2是桃子果实蔗糖分解代谢关键的酸性液泡转化酶（VIN）的关键基因。酵母单杂交（Y1H）、电泳迁移率转移（EMSA）和双荧光素酶报告基因（DLR）实验共同发现PpMYC2直接结合PpVIN2启动子中的G-box顺式元件，并显著抑制其启动子活性。桃果实中PpMYC2的瞬时过表达导致PpVIN2转录下调，VIN活性降低，蔗糖含量增加，而PpMYC2的瞬时沉默则导致相反的变化。此外，我们还利用农杆菌介导的转化方法稳定地培养了过表达PpMYC2的番茄植株，并观察到过表达PpMYC2的番茄果实具有显著增强的耐寒性，同时抑制了蔗糖的降解。综上所述，这些发现表明PpMYC2在低温胁迫下作为PpVIN2的转录抑制因子，从而减少蔗糖分解，增强桃果的抗寒性。本研究确定PpMYC2是缓解桃果冷害的潜在分子靶点。

{"title":"PpMYC2 positively regulates chilling tolerance of peach fruit by suppressing sucrose degradation","authors":"Qian Li , Yingying Wei , Yi Chen , Shu Jiang , Jianfen Ye , Fangming Wu , Feng Xu , Xingfeng Shao","doi":"10.1016/j.postharvbio.2026.114179","DOIUrl":"10.1016/j.postharvbio.2026.114179","url":null,"abstract":"<div><div>MYC2 transcription factor has been extensively investigated in plant stress responses. However, its role in regulating fruit cold tolerance remains largely unexplored. In this study, exposure to cold stress rapidly activated the transcription of <em>PpMYC2</em> in peach fruit. DNA affinity purification sequencing (DAP-seq) data revealed that PpMYC2 showed high affinity for the promoter region of <em>PpVIN2</em> - a key gene encoding acidic vacuolar invertase (VIN) critical for sucrose catabolism in peach fruit. Yeast one-hybridization (Y1H), electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter (DLR) assay collectively revealed that PpMYC2 directly bound to the G-box cis-element in the <em>PpVIN2</em> promoter and significantly suppressed its promoter activity. Transient overexpression of <em>PpMYC2</em> in peach fruit resulted in downregulated transcription of <em>PpVIN2</em>, reduced VIN activity, and increased sucrose content, whereas opposite changes were detected in peach fruit with transient silencing of <em>PpMYC2</em>. Additionally, we generated tomato plants stably overexpressing <em>PpMYC2</em> using <em>Agrobacterium</em>-mediated transformation, and observed that <em>PpMYC2</em>-overexpressing tomato fruit exhibited significantly enhanced cold tolerance, accompanied by the inhibition of sucrose degradation. Collectively, these discoveries indicate that PpMYC2 acts as a transcriptional repressor of <em>PpVIN2</em> under cold stress, thereby reducing sucrose breakdown and enhancing peach fruit chilling tolerance. This study identifies <em>PpMYC2</em> as a potential molecular target for alleviating chilling injury in peach fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114179"},"PeriodicalIF":6.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of curcumin mediated carbon cycle network and energy metabolism to improve the storage quality and flavor of pepper 姜黄素介导的碳循环网络和能量代谢改善辣椒贮藏品质和风味的机制

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2026-01-17 DOI: 10.1016/j.postharvbio.2026.114164

Wenjuan Xu , Yansheng Wang , Chengli Jia , Zhiqing Gong , Jian Zhang , Fengjuan Jia , Wenjia Cui , Shasha Song , Xiaochun Qin , Wenliang Wang , Junyan Shi

Curcumin (CUR) is a natural food preservative known for its various biological activities. This study systematically examined its effects on the physiological quality and flavor characteristics of postharvest chili peppers. The results showed that treating the peppers with 2 g L⁻¹ CUR effectively delayed fruit reddening, preserved nutritional quality, and inhibited postharvest deterioration. A significant enhancement in richness was observed in the CUR group via the electronic tongue compared to the CK group. Additionally, non-targeted volatile compound analysis conducted through gas chromatography-mass spectrometry (GC-MS) revealed that CUR significantly increased the abundance of 35 volatile flavor compounds relative to the CK group. Relative Odor Activity Value (ROAV) analysis highlighted 2-methoxy-3-(2-methylpropyl) pyrazine as the key characteristic flavor compound found in the CUR group, which was absent in the CK group. This observation confirms the role of CUR in shaping the key flavor profiles of chili peppers. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that most of the differential volatile substances were enriched in the core metabolic pathways associated with carbon cycling, including glycolysis/gluconeogenesis and pyruvate metabolism. These findings illuminate the potential metabolic mechanisms by which CUR enhances flavor quality in chili peppers from a pathway perspective. Overall, this study provides a theoretical basis for using CUR as a natural agent that offers both preservative and flavor-enhancing properties in the postharvest preservation of fruits and vegetables.

姜黄素（Curcumin， CUR）是一种天然的食品防腐剂，具有多种生物活性。本研究系统地考察了其对采后辣椒生理品质和风味特性的影响。结果表明，2 g L−1 CUR处理能有效延缓果实变红，保留营养品质，抑制采后变质。与CK组相比，通过电子舌观察到CUR组丰富度的显著增强。此外，通过气相色谱-质谱（GC-MS）进行的非靶向挥发性化合物分析显示，相对于CK组，CUR显著增加了35种挥发性风味化合物的丰度。相对气味活性值（ROAV）分析显示，2-甲氧基-3-（2-甲基丙基）吡嗪是CUR组中发现的关键特征风味化合物，而CK组中没有。这一观察证实了CUR在形成辣椒的关键风味特征中的作用。此外，京都基因与基因组百科（KEGG）途径富集分析表明，大多数差异挥发性物质富集于与碳循环相关的核心代谢途径，包括糖酵解/糖异生和丙酮酸代谢。这些发现从途径的角度阐明了CUR提高辣椒风味质量的潜在代谢机制。综上所述，本研究为将CUR作为一种既具有防腐性能又具有增味性能的天然试剂用于果蔬采后保鲜提供了理论基础。

{"title":"Mechanism of curcumin mediated carbon cycle network and energy metabolism to improve the storage quality and flavor of pepper","authors":"Wenjuan Xu , Yansheng Wang , Chengli Jia , Zhiqing Gong , Jian Zhang , Fengjuan Jia , Wenjia Cui , Shasha Song , Xiaochun Qin , Wenliang Wang , Junyan Shi","doi":"10.1016/j.postharvbio.2026.114164","DOIUrl":"10.1016/j.postharvbio.2026.114164","url":null,"abstract":"<div><div>Curcumin (CUR) is a natural food preservative known for its various biological activities. This study systematically examined its effects on the physiological quality and flavor characteristics of postharvest chili peppers. The results showed that treating the peppers with 2 g L<sup>−1</sup> CUR effectively delayed fruit reddening, preserved nutritional quality, and inhibited postharvest deterioration. A significant enhancement in richness was observed in the CUR group via the electronic tongue compared to the CK group. Additionally, non-targeted volatile compound analysis conducted through gas chromatography-mass spectrometry (GC-MS) revealed that CUR significantly increased the abundance of 35 volatile flavor compounds relative to the CK group. Relative Odor Activity Value (ROAV) analysis highlighted 2-methoxy-3-(2-methylpropyl) pyrazine as the key characteristic flavor compound found in the CUR group, which was absent in the CK group. This observation confirms the role of CUR in shaping the key flavor profiles of chili peppers. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that most of the differential volatile substances were enriched in the core metabolic pathways associated with carbon cycling, including glycolysis/gluconeogenesis and pyruvate metabolism. These findings illuminate the potential metabolic mechanisms by which CUR enhances flavor quality in chili peppers from a pathway perspective. Overall, this study provides a theoretical basis for using CUR as a natural agent that offers both preservative and flavor-enhancing properties in the postharvest preservation of fruits and vegetables.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114164"},"PeriodicalIF":6.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcium chloride inhibits water-soaking disorder of fresh-cut cantaloupe through regulating sugar, organic acid, and anaerobic respiration metabolisms 氯化钙通过调节糖、有机酸和无氧呼吸代谢来抑制鲜切哈密瓜的浸水失调

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-04-01 Epub Date: 2025-12-12 DOI: 10.1016/j.postharvbio.2025.114125

Wanli You , Jinglin Zhang , Xueyin Ru , Feng Xu , Zhengguo Wu , Peng Jin , Yonghua Zheng , Shifeng Cao

The effect of exogenous calcium chloride (CaCl₂) treatment on water-soaking disorder inhibition and quality maintenance in fresh-cut cantaloupe was studied. Results indicated that CaCl₂ preserved visual appearance, microbiological safety, and nutrient composition. Transcriptome analysis identified 5416 differentially expressed genes and most were enriched in sugar-related metabolisms. Moreover, less reducing sugar accumulation including glucose and fructose and lower activities of acidic invertase (AI) and neutral invertase (NI), as well as expressions of CmAI1 and CmNI, were resulted from CaCl₂ treatment. Additionally, CaCl₂ treatment retained more organic acid and inhibited anaerobic respiration through downregulating corresponding expressions and enzyme activities of pyruvate decarboxylase, alcohol dehydrogenase, and lactate dehydrogenase, as well as reducing the accumulation of anaerobic metabolites including ethanol, acetaldehyde, and pyruvate. Therefore, these findings implied that CaCl₂ application was practical for storage performance improvement and water-soaking disorder inhibition via modulating sugar, organic acid, and anaerobic respiration metabolism of fresh-cut cantaloupe, thus serving as a theoretical basis for fresh-cut product processing and preservation.

研究了外源氯化钙（CaCl2）处理对鲜切甜瓜水浸失调抑制和品质维持的影响。结果表明，CaCl2保存了食品的外观、微生物安全性和营养成分。转录组分析鉴定出5416个差异表达基因，其中大多数富集于糖相关代谢。此外，CaCl2还减少了葡萄糖和果糖等还原糖的积累，降低了酸性转化酶（AI）和中性转化酶（NI）的活性以及CmAI1和CmNI的表达。此外，CaCl2处理通过下调丙酮酸脱羧酶、乙醇脱氢酶和乳酸脱氢酶的相应表达和酶活性，以及减少乙醇、乙醛和丙酮酸等厌氧代谢物的积累，保留了更多的有机酸，抑制了厌氧呼吸。研究结果表明，CaCl2通过调节甜瓜的糖、有机酸和厌氧呼吸代谢，可以提高甜瓜的贮藏性能，抑制水浸紊乱，为甜瓜加工保鲜提供理论依据。

{"title":"Calcium chloride inhibits water-soaking disorder of fresh-cut cantaloupe through regulating sugar, organic acid, and anaerobic respiration metabolisms","authors":"Wanli You , Jinglin Zhang , Xueyin Ru , Feng Xu , Zhengguo Wu , Peng Jin , Yonghua Zheng , Shifeng Cao","doi":"10.1016/j.postharvbio.2025.114125","DOIUrl":"10.1016/j.postharvbio.2025.114125","url":null,"abstract":"<div><div>The effect of exogenous calcium chloride (CaCl<sub>2</sub>) treatment on water-soaking disorder inhibition and quality maintenance in fresh-cut cantaloupe was studied. Results indicated that CaCl<sub>2</sub> preserved visual appearance, microbiological safety, and nutrient composition. Transcriptome analysis identified 5416 differentially expressed genes and most were enriched in sugar-related metabolisms. Moreover, less reducing sugar accumulation including glucose and fructose and lower activities of acidic invertase (AI) and neutral invertase (NI), as well as expressions of <em>CmAI1</em> and <em>CmNI</em>, were resulted from CaCl<sub>2</sub> treatment. Additionally, CaCl<sub>2</sub> treatment retained more organic acid and inhibited anaerobic respiration through downregulating corresponding expressions and enzyme activities of pyruvate decarboxylase, alcohol dehydrogenase, and lactate dehydrogenase, as well as reducing the accumulation of anaerobic metabolites including ethanol, acetaldehyde, and pyruvate. Therefore, these findings implied that CaCl<sub>2</sub> application was practical for storage performance improvement and water-soaking disorder inhibition via modulating sugar, organic acid, and anaerobic respiration metabolism of fresh-cut cantaloupe, thus serving as a theoretical basis for fresh-cut product processing and preservation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114125"},"PeriodicalIF":6.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145737317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0