Pub Date : 2024-10-29DOI: 10.1016/j.postharvbio.2024.113291
Jiaxin Wang , Jiatong Li , Wei Chen , Zhenfeng Yang , Xuewen Li , Li Wang , Shifeng Cao , Liyu Shi
The changes in chlorophyll, solanine, and plant hormones during greening in postharvest potatoes exposed to light were investigated. The results showed that as potatoes under light turned green, the contents of chlorophyll and solanine sharply increased due to the upregulation of genes involved in their biosynthesis. At the end of storage, the chlorophyll and solanine content in potatoes stored under light were approximately 25.44 times and 1.62 times higher, respectively, compared to those stored in the dark. Light exposure inhibited the accumulation of indole-3-acetic acid (IAA) and gibberellin in potatoes, which was associated with the downregulation of their anabolic genes and the positive regulation of their catabolic genes. In contrast, light treatment increased the levels of ethylene, melatonin, brassinosteroid, abscisic acid and 6-benzylaminopurine compared to dark-stored potatoes. Correlation analysis revealed that solanine content was negatively correlated with IAA (r = −0.93) during greening, while chlorophyll content was positively associated with ethylene production (r = 0.91) but negatively related to IAA (r = −0.89). Our results suggested potential roles of phytohormones in regulating chlorophyll and solanine during greening in light exposed potatoes, which deserves further investigation.
{"title":"The changes in chlorophyll, solanine, and phytohormones during light-induced greening in postharvest potatoes","authors":"Jiaxin Wang , Jiatong Li , Wei Chen , Zhenfeng Yang , Xuewen Li , Li Wang , Shifeng Cao , Liyu Shi","doi":"10.1016/j.postharvbio.2024.113291","DOIUrl":"10.1016/j.postharvbio.2024.113291","url":null,"abstract":"<div><div>The changes in chlorophyll, solanine, and plant hormones during greening in postharvest potatoes exposed to light were investigated. The results showed that as potatoes under light turned green, the contents of chlorophyll and solanine sharply increased due to the upregulation of genes involved in their biosynthesis. At the end of storage, the chlorophyll and solanine content in potatoes stored under light were approximately 25.44 times and 1.62 times higher, respectively, compared to those stored in the dark. Light exposure inhibited the accumulation of indole-3-acetic acid (IAA) and gibberellin in potatoes, which was associated with the downregulation of their anabolic genes and the positive regulation of their catabolic genes. In contrast, light treatment increased the levels of ethylene, melatonin, brassinosteroid, abscisic acid and 6-benzylaminopurine compared to dark-stored potatoes. Correlation analysis revealed that solanine content was negatively correlated with IAA (r = −0.93) during greening, while chlorophyll content was positively associated with ethylene production (r = 0.91) but negatively related to IAA (r = −0.89). Our results suggested potential roles of phytohormones in regulating chlorophyll and solanine during greening in light exposed potatoes, which deserves further investigation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113291"},"PeriodicalIF":6.4,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-26DOI: 10.1016/j.postharvbio.2024.113281
Dengjie Chen , Fan Lin , Caihua Lu , JunWei Zhuang , Hongjie Su , Dehui Zhang , Jincheng He
Passion fruit, a commercially significant fruit crop, is easily infected by anthracnose and scab, which declines it economic value. However, at the present time, passion fruit quality grading is mainly judged by manual assessment, with strong subjectivity, poor efficiency and low accuracy. Intelligent classification of postharvest passion fruit is essential, with skin disease being a critical factor in grading fruit quality. In view of the shortcomings in traditional deep learning model, such as weak multi-scale detection ability and low accuracy, we propose a YOLOv8-MDN-Tiny model to improve the ability of passion fruit small-scale disease detection. The backbone layer is replaced by the self-made MFSO structure to expand the feature pixels of small target information and enrich their feature expression. An improved DyRep module is proposed to realize the interactive fusion of disease features at different scales and depths. NWD loss function is introduced to accurately measure the overlap of two bounding boxes. Finally, Slimming pruning and CWD are used to compress the model. Compared with YOLOv8s, our improved lightweight model achieves more accurate localization of small passion fruit targets. Specifically, the mAP50 is increased by 2.2–94.8 %, the precision and recall are improved by 1.5 % and 6.0 %. Meanwhile, the number of model parameters and memory usage are decreased by 90.1 % and 88.9 %. The results technically support the disease detection in postharvest passion fruit and real-time grading of their quality.
{"title":"YOLOv8-MDN-Tiny: A lightweight model for multi-scale disease detection of postharvest golden passion fruit","authors":"Dengjie Chen , Fan Lin , Caihua Lu , JunWei Zhuang , Hongjie Su , Dehui Zhang , Jincheng He","doi":"10.1016/j.postharvbio.2024.113281","DOIUrl":"10.1016/j.postharvbio.2024.113281","url":null,"abstract":"<div><div>Passion fruit, a commercially significant fruit crop, is easily infected by anthracnose and scab, which declines it economic value. However, at the present time, passion fruit quality grading is mainly judged by manual assessment, with strong subjectivity, poor efficiency and low accuracy. Intelligent classification of postharvest passion fruit is essential, with skin disease being a critical factor in grading fruit quality. In view of the shortcomings in traditional deep learning model, such as weak multi-scale detection ability and low accuracy, we propose a YOLOv8-MDN-Tiny model to improve the ability of passion fruit small-scale disease detection. The backbone layer is replaced by the self-made MFSO structure to expand the feature pixels of small target information and enrich their feature expression. An improved DyRep module is proposed to realize the interactive fusion of disease features at different scales and depths. NWD loss function is introduced to accurately measure the overlap of two bounding boxes. Finally, Slimming pruning and CWD are used to compress the model. Compared with YOLOv8s, our improved lightweight model achieves more accurate localization of small passion fruit targets. Specifically, the mAP<sub>50</sub> is increased by 2.2–94.8 %, the precision and recall are improved by 1.5 % and 6.0 %. Meanwhile, the number of model parameters and memory usage are decreased by 90.1 % and 88.9 %. The results technically support the disease detection in postharvest passion fruit and real-time grading of their quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113281"},"PeriodicalIF":6.4,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-25DOI: 10.1016/j.postharvbio.2024.113277
Yiran Yan , Yetong Feng , Tao Chen , Qiuling Shao , Nan Wu , Shuai Han , Jie Liu , Dingkang Wei , Yang Shen , Xinyu Zhang , Yang Li , Dehui Qu , Ying Zhu , Guochao Wu
Cut flowers of Rosa hybrida 'Lychee' are susceptible to rapid decay, resulting in a brief shelf life, a inherent feature to their floral physiology. This study evaluated the effects of bacterial nanocellulose (BNC), which was produced from the legume processing wastewater and loaded with Auricularia auricula polysaccharide and 8-hydroxyquinoline, in extending the shelf life of these cut rose flowers. Water-retaining cotton and floral foam blocks were employed as control materials. The bacterial strain Komagataeibacter rhaeticus CP050139.1 yielded BNC from legume waste at a rate comparable to that from a glucose-based medium: 0.107±0.004 g L−1 d−1 versus 0.157±0.004 g L−1 d−1, respectively. Characterization via FE-SEM, FT-IR, TGA, and XRD confirmed that the BNC from both sources exhibited similar microscopic morphology, chemical composition, thermal stability, and crystallinity. The use of BNC, particularly when enriched with Auricularia auricula polysaccharide (AAP), extended the shelf life of cut roses by 50 % and significantly minimized fresh weight loss in comparison to the control materials. BNC's superior water-holding capacity — 42.7 times greater than that of water-retaining cotton — proved advantages for maintaining hydration and preventing wilting during transportation. This study underscored the potential of BNC hydrogel, sourced from legume processing wastewater, as an innovative, effective, and environmentally sustainable solution for preserving cut rose flowers, promoting the recycling of agricultural by products and enhancing the preservation of horticultural products.
{"title":"Enhanced preservation of cut rose flowers through bacterial nanocellulose produced from legume wastewater and fortified with Auricularia auricula polysaccharide","authors":"Yiran Yan , Yetong Feng , Tao Chen , Qiuling Shao , Nan Wu , Shuai Han , Jie Liu , Dingkang Wei , Yang Shen , Xinyu Zhang , Yang Li , Dehui Qu , Ying Zhu , Guochao Wu","doi":"10.1016/j.postharvbio.2024.113277","DOIUrl":"10.1016/j.postharvbio.2024.113277","url":null,"abstract":"<div><div>Cut flowers of <em>Rosa hybrida</em> 'Lychee' are susceptible to rapid decay, resulting in a brief shelf life, a inherent feature to their floral physiology. This study evaluated the effects of bacterial nanocellulose (BNC), which was produced from the legume processing wastewater and loaded with <em>Auricularia auricula</em> polysaccharide and 8-hydroxyquinoline, in extending the shelf life of these cut rose flowers. Water-retaining cotton and floral foam blocks were employed as control materials. The bacterial strain <em>Komagataeibacter rhaeticus</em> CP050139.1 yielded BNC from legume waste at a rate comparable to that from a glucose-based medium: 0.107±0.004 g L<sup>−1</sup> d<sup>−1</sup> versus 0.157±0.004 g L<sup>−1</sup> d<sup>−1</sup>, respectively. Characterization via FE-SEM, FT-IR, TGA, and XRD confirmed that the BNC from both sources exhibited similar microscopic morphology, chemical composition, thermal stability, and crystallinity. The use of BNC, particularly when enriched with <em>Auricularia auricula</em> polysaccharide (AAP), extended the shelf life of cut roses by 50 % and significantly minimized fresh weight loss in comparison to the control materials. BNC's superior water-holding capacity — 42.7 times greater than that of water-retaining cotton — proved advantages for maintaining hydration and preventing wilting during transportation. This study underscored the potential of BNC hydrogel, sourced from legume processing wastewater, as an innovative, effective, and environmentally sustainable solution for preserving cut rose flowers, promoting the recycling of agricultural by products and enhancing the preservation of horticultural products.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113277"},"PeriodicalIF":6.4,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-23DOI: 10.1016/j.postharvbio.2024.113271
Jinmei Wei, Yongmei Zhang, Wenli Li
The Lanzhou bulb of lily has gained global attention due to its high culinary and medicinal value. However, the quality characteristics of lily bulbs during storage remain unclear. Hence, to clarify the dynamic changes in the physiochemical characteristics of the bulbs during storage, this study analyzed bulbs at 0-, 15-, 30-, 45-, and 60-day vacuum and cold storage, respectively. The results revealed that the water content and pH decreased, while soluble sugar and dietary fiber contents fluctuated. Soluble protein was less affected by the storage time, yet starch was observed to degrade after 45 d of storage. A total of 66 volatile compounds were identified, among them, seven volatiles were dominant across all five storage periods. Fresh Lanzhou lily bulbs (0-day) contained higher water and sucrose contents. During the 45-day storage period, the quality of the lily bulbs was greatly influenced by the contents of starch, total dietary fiber (TDF), soluble dietary fiber (SDF), and esters, and by the contents of fructose, aldehydes, ketones, alkenes, and alkanes after 60 d of storage. Under the combination of vacuum packing and low-temperature storage, the optimal storage of Lanzhou lily bulbs is 45 d. This work is expected to be helpful in evaluating the quality of Lanzhou lily bulbs during storage and promoting the development of the lily industry.
{"title":"Comparison of physiochemical characteristics of Lanzhou bulb of lily during storage periods","authors":"Jinmei Wei, Yongmei Zhang, Wenli Li","doi":"10.1016/j.postharvbio.2024.113271","DOIUrl":"10.1016/j.postharvbio.2024.113271","url":null,"abstract":"<div><div>The Lanzhou bulb of lily has gained global attention due to its high culinary and medicinal value. However, the quality characteristics of lily bulbs during storage remain unclear. Hence, to clarify the dynamic changes in the physiochemical characteristics of the bulbs during storage, this study analyzed bulbs at 0-, 15-, 30-, 45-, and 60-day vacuum and cold storage, respectively. The results revealed that the water content and pH decreased, while soluble sugar and dietary fiber contents fluctuated. Soluble protein was less affected by the storage time, yet starch was observed to degrade after 45 d of storage. A total of 66 volatile compounds were identified, among them, seven volatiles were dominant across all five storage periods. Fresh Lanzhou lily bulbs (0-day) contained higher water and sucrose contents. During the 45-day storage period, the quality of the lily bulbs was greatly influenced by the contents of starch, total dietary fiber (TDF), soluble dietary fiber (SDF), and esters, and by the contents of fructose, aldehydes, ketones, alkenes, and alkanes after 60 d of storage. Under the combination of vacuum packing and low-temperature storage, the optimal storage of Lanzhou lily bulbs is 45 d. This work is expected to be helpful in evaluating the quality of Lanzhou lily bulbs during storage and promoting the development of the lily industry.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113271"},"PeriodicalIF":6.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Moldy core in apples is a common disease, with early symptoms not visible on the fruit surface. When affected apples are mixed with healthy ones, overall fruit quality declines, leading to the decay of healthy apples. Therefore, there is an urgent need for a rapid, non-destructive detection method. However, variations in apple diameter significantly affect the intensity of NIR transmission spectra, impacting the accuracy of detecting moldy core in apples. To address this issue, various diameter correction methods including a novel method we proposed were employed in this study to improve the accuracy of near-infrared detection of moldy core in apples, and these methods were also compared. The results indicate that the moldy core classification model is significantly influenced by apple diameter, with the uncorrected model achieving only 83.64 % accuracy in the prediction set. After adopting the diameter information fusion correction method, the performance of model has been slightly improved, with the accuracy of prediction set increasing by 0.91 %. Further improvement is achieved when using spectral normalization based on correlation and spectral correction based on diameter transformation, which has raised the accuracy of prediction set to 86.36 %. And the spectral correction based on polynomial transformation method proposed in this study has significantly improved the model performance, with the calibration and prediction sets achieving sensitivity, specificity, and accuracy of 85.22 %, 95.24 %, 90.00 %, and 85.45 %, 92.7 %, 89.09 %, respectively. Compared to the uncorrected model, the accuracy of the model in prediction set has been improved by 5.45 %. The model also demonstrates a 4.54 % enhancement over the one corrected using the diameter information fusion method. Additionally, when evaluated against the models using spectral normalization based on correlation and spectral correction based on diameter transformation, the accuracy has increased by 2.73 %. Therefore, the method of spectral correction based on polynomial transformation that proposed in this study effectively reduces the impact of apple diameter on transmission spectra, improving the detection accuracy of moldy core in apples and supporting rapid, non-destructive, high-precision detection.
{"title":"Improving the accuracy of NIR detection of moldy core in apples using different diameter correction methods","authors":"Hanlin Li, Jiajun Zan, Linxin Zhang, Binyan Hou, Tong Sun, Dong Hu","doi":"10.1016/j.postharvbio.2024.113279","DOIUrl":"10.1016/j.postharvbio.2024.113279","url":null,"abstract":"<div><div>Moldy core in apples is a common disease, with early symptoms not visible on the fruit surface. When affected apples are mixed with healthy ones, overall fruit quality declines, leading to the decay of healthy apples. Therefore, there is an urgent need for a rapid, non-destructive detection method. However, variations in apple diameter significantly affect the intensity of NIR transmission spectra, impacting the accuracy of detecting moldy core in apples. To address this issue, various diameter correction methods including a novel method we proposed were employed in this study to improve the accuracy of near-infrared detection of moldy core in apples, and these methods were also compared. The results indicate that the moldy core classification model is significantly influenced by apple diameter, with the uncorrected model achieving only 83.64 % accuracy in the prediction set. After adopting the diameter information fusion correction method, the performance of model has been slightly improved, with the accuracy of prediction set increasing by 0.91 %. Further improvement is achieved when using spectral normalization based on correlation and spectral correction based on diameter transformation, which has raised the accuracy of prediction set to 86.36 %. And the spectral correction based on polynomial transformation method proposed in this study has significantly improved the model performance, with the calibration and prediction sets achieving sensitivity, specificity, and accuracy of 85.22 %, 95.24 %, 90.00 %, and 85.45 %, 92.7 %, 89.09 %, respectively. Compared to the uncorrected model, the accuracy of the model in prediction set has been improved by 5.45 %. The model also demonstrates a 4.54 % enhancement over the one corrected using the diameter information fusion method. Additionally, when evaluated against the models using spectral normalization based on correlation and spectral correction based on diameter transformation, the accuracy has increased by 2.73 %. Therefore, the method of spectral correction based on polynomial transformation that proposed in this study effectively reduces the impact of apple diameter on transmission spectra, improving the detection accuracy of moldy core in apples and supporting rapid, non-destructive, high-precision detection.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113279"},"PeriodicalIF":6.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-23DOI: 10.1016/j.postharvbio.2024.113274
Athakorn Promwee , Narumol Matan
The transportation and storage of chili in cold storage are beneficial for handling from farm to household; however, browning injury and quality loss during cold storage are the main concerns. This study explored a new method for preharvest chili plants to reduce browning injury and maintain postharvest quality during cold storage (4 ± 2 °C) for at least 40 days. Citrus essential oil (lime oil and orange oil) nanoemulsion at a low concentration of 0.006 % v v−1 was applied to chili plants for 10 days before the 1st harvest at 90 days and the 2nd harvest at 120 days. Both the first and second harvest chili fruits were kept under cold storage for 40 days. Color change, pigment, bioactive compounds, and quality of chili were measured. Results showed that citrus nanoemulsion could be absorbed from the roots of chili plants and pass through the flowers and fruits. The treated chilies were firmer, more water-resistant, and had a longer fruit length than the control. Treated chili also displayed delayed color change, ripening from green to red, increased brightness, and reduced browning injury on both the surface and seeds. Furthermore, higher total phenolic content, total flavonoid content, and antioxidant activity were confirmed in the treated chili, which helped to maintain the quality of the chili fruit from 20 days (control) to 40 days, with a slight disease incidence (17 %) and low weight loss (7 %) compared to the control (54 % disease incidence and 64 % weight loss). Orange oil nanoemulsion showed greater quality preservation than lime oil due to the higher limonene content found on the chili surface, around 5.7 % for chili treated with orange oil compared to 0.7 % for lime oil. This finding indicates a method to reduce browning injury and prolong the shelf life of chili during cold storage. It is easy to implement in chili farming, allowing for at least two harvests before the next pretreatment, benefiting both farmers and consumers.
{"title":"Revealing the mechanism underlying the use of preharvest nano-emulsion of citrus essential oil for reducing browning injury and maintaining postharvest quality of chili during cold storage","authors":"Athakorn Promwee , Narumol Matan","doi":"10.1016/j.postharvbio.2024.113274","DOIUrl":"10.1016/j.postharvbio.2024.113274","url":null,"abstract":"<div><div>The transportation and storage of chili in cold storage are beneficial for handling from farm to household; however, browning injury and quality loss during cold storage are the main concerns. This study explored a new method for preharvest chili plants to reduce browning injury and maintain postharvest quality during cold storage (4 ± 2 °C) for at least 40 days. Citrus essential oil (lime oil and orange oil) nanoemulsion at a low concentration of 0.006 % v v<sup>−1</sup> was applied to chili plants for 10 days before the 1st harvest at 90 days and the 2nd harvest at 120 days. Both the first and second harvest chili fruits were kept under cold storage for 40 days. Color change, pigment, bioactive compounds, and quality of chili were measured. Results showed that citrus nanoemulsion could be absorbed from the roots of chili plants and pass through the flowers and fruits. The treated chilies were firmer, more water-resistant, and had a longer fruit length than the control. Treated chili also displayed delayed color change, ripening from green to red, increased brightness, and reduced browning injury on both the surface and seeds. Furthermore, higher total phenolic content, total flavonoid content, and antioxidant activity were confirmed in the treated chili, which helped to maintain the quality of the chili fruit from 20 days (control) to 40 days, with a slight disease incidence (17 %) and low weight loss (7 %) compared to the control (54 % disease incidence and 64 % weight loss). Orange oil nanoemulsion showed greater quality preservation than lime oil due to the higher limonene content found on the chili surface, around 5.7 % for chili treated with orange oil compared to 0.7 % for lime oil. This finding indicates a method to reduce browning injury and prolong the shelf life of chili during cold storage. It is easy to implement in chili farming, allowing for at least two harvests before the next pretreatment, benefiting both farmers and consumers.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113274"},"PeriodicalIF":6.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-23DOI: 10.1016/j.postharvbio.2024.113278
Meiying He , Feilong Yin , Mohd Sabri Pak Dek , Lingyan Liao , Yunfen Liu , Yuanli Liang , Wen Cai , Liangliang Huang , Liang Shuai
The market value of litchi fruit decreases due to pericarp browning. Methyl jasmonate (MeJA) has the ability to inhibit the occurrence of browning in litchi pericarp after harvest. However, the molecular mechanism underlying this effect remains unknown. In the present study, we used 100 μM MeJA to treat the ‘Guiwei’ litchi fruit to investigate the molecular mechanism by which MeJA delays the occurrence of browning via the phenylpropanoid metabolism. The results demonstrated that MeJA treatment effectively decreased the accumulation of reactive oxygen species (ROS), relative leakage rate (Rlr), and malondialdehyde (MDA) content. Additionally, MeJA treatment enhanced the enzyme activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-monooxygenase (C4H), and 4-coumarate: coenzyme A ligase (4CL) in the early stage of phenylpropanoid metabolism, and up-regulated the expression of their respective genes, resulting in an increased content of GC, EGC, EGCG, EC, ECG, CG, total phenolics and flavonoids that help eliminate excess ROS and maintain a high antioxidant capacity. Treatment of fruit with MeJA down-regulated the gene expression and enzyme activity of peroxidase (POD) and decelerated the decline in the content of anthocyanins. Interestingly, MeJA treatment induced both the activity and gene expression of polyphenol oxidase (PPO). Correlation analysis showed that pericarp browning is closely related to PAL, C4H, 4CL, and phenolic components. In conclusion, MeJA treatment activated the early stage of the phenylpropanoid metabolism pathway, enhanced antioxidant capacity, and maintained anthocyanins content in the pericarp, thus delaying the browning of litchi fruit during storage at 4 ℃. These findings will lay the foundation for the application of MeJA in fruit preservation.
荔枝果实的市场价值会因果皮褐变而降低。茉莉酸甲酯（MeJA）能够抑制荔枝果皮在采收后发生褐变。然而，这种作用的分子机制仍不清楚。本研究采用 100 μM MeJA 处理 "桂味 "荔枝果实，研究 MeJA 通过苯丙类代谢延缓褐变发生的分子机制。结果表明，MeJA 处理能有效降低活性氧（ROS）的积累、相对漏失率（Rlr）和丙二醛（MDA）的含量。此外，MeJA 处理还提高了苯丙氨酸氨基转移酶（PAL）、肉桂酸-4-单加氧酶（C4H）和 4-香豆酸的酶活性：在苯丙类代谢的早期阶段，MeJA 还能提高辅酶 A 连接酶（4CL）的活性，并上调它们各自基因的表达，从而增加 GC、EGC、EGCG、EC、ECG、CG、总酚类和类黄酮的含量，有助于消除过量的 ROS 并保持较高的抗氧化能力。用 MeJA 处理水果会降低过氧化物酶（POD）的基因表达和酶活性，并减缓花青素含量的下降。有趣的是，MeJA 处理可诱导多酚氧化酶（PPO）的活性和基因表达。相关分析表明，果皮褐变与 PAL、C4H、4CL 和酚类成分密切相关。总之，MeJA 处理激活了苯丙类代谢途径的早期阶段，提高了抗氧化能力，维持了果皮中花青素的含量，从而延缓了荔枝果实在 4 ℃ 贮藏期间的褐变。这些发现将为 MeJA 在水果保鲜中的应用奠定基础。
{"title":"Methyl jasmonate delays the browning of litchi pericarp by activating the phenylpropanoid metabolism during cold storage","authors":"Meiying He , Feilong Yin , Mohd Sabri Pak Dek , Lingyan Liao , Yunfen Liu , Yuanli Liang , Wen Cai , Liangliang Huang , Liang Shuai","doi":"10.1016/j.postharvbio.2024.113278","DOIUrl":"10.1016/j.postharvbio.2024.113278","url":null,"abstract":"<div><div>The market value of litchi fruit decreases due to pericarp browning. Methyl jasmonate (MeJA) has the ability to inhibit the occurrence of browning in litchi pericarp after harvest. However, the molecular mechanism underlying this effect remains unknown. In the present study, we used 100 μM MeJA to treat the ‘Guiwei’ litchi fruit to investigate the molecular mechanism by which MeJA delays the occurrence of browning via the phenylpropanoid metabolism. The results demonstrated that MeJA treatment effectively decreased the accumulation of reactive oxygen species (ROS), relative leakage rate (Rlr), and malondialdehyde (MDA) content. Additionally, MeJA treatment enhanced the enzyme activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-monooxygenase (C4H), and 4-coumarate: coenzyme A ligase (4CL) in the early stage of phenylpropanoid metabolism, and up-regulated the expression of their respective genes, resulting in an increased content of GC, EGC, EGCG, EC, ECG, CG, total phenolics and flavonoids that help eliminate excess ROS and maintain a high antioxidant capacity. Treatment of fruit with MeJA down-regulated the gene expression and enzyme activity of peroxidase (POD) and decelerated the decline in the content of anthocyanins. Interestingly, MeJA treatment induced both the activity and gene expression of polyphenol oxidase (PPO). Correlation analysis showed that pericarp browning is closely related to PAL, C4H, 4CL, and phenolic components. In conclusion, MeJA treatment activated the early stage of the phenylpropanoid metabolism pathway, enhanced antioxidant capacity, and maintained anthocyanins content in the pericarp, thus delaying the browning of litchi fruit during storage at 4 ℃. These findings will lay the foundation for the application of MeJA in fruit preservation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113278"},"PeriodicalIF":6.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-23DOI: 10.1016/j.postharvbio.2024.113273
Yuxuan Li , Jiaqi Wang , Ling Yang , Bingxin Sun , Yunhe Zhang , Yufeng Xu , Xuerui Yan
Fumigation with terpinen-4-ol (4OL) could enhance blueberry quality during postharvest storage in advance study. This work investigated the response of blueberry fruits to 4OL treatment based on transcriptomics. VcSPS2 was noticed not only by significant up-regulation but also due to its relation to sucrose metabolic pathways. Following this clue, the enzymes and genes related to sucrose metabolism pathway were tested on blueberry fruits after 4OL fumigation. The result showed that sucrose content 28 % improved, the sucrose synthesis genes expression up-regulated and enzymes activity increased, the sucrose degradation genes expression down-regulated and enzymes activity suppressed. Meanwhile, VcGLU3 associated with cell wall degradation was observed significantly down-regulated in transcriptomics. Moreover, the enzymes and genes related to cell wall degradation were verified and blueberry firmness were checked. The result indicated that firmness maintained well and the cell wall degradation genes expression down-regulated and enzymes activity suppressed. It indicated that 4OL could be a novel preservative in quality enhancement and retardation blueberry postharvest softening.
{"title":"Transcriptomics revealed terpinen-4-ol mediated sucrose metabolism and delayed cell wall degradation to maintain blueberry quality","authors":"Yuxuan Li , Jiaqi Wang , Ling Yang , Bingxin Sun , Yunhe Zhang , Yufeng Xu , Xuerui Yan","doi":"10.1016/j.postharvbio.2024.113273","DOIUrl":"10.1016/j.postharvbio.2024.113273","url":null,"abstract":"<div><div>Fumigation with terpinen-4-ol (4OL) could enhance blueberry quality during postharvest storage in advance study. This work investigated the response of blueberry fruits to 4OL treatment based on transcriptomics. <em>VcSPS2</em> was noticed not only by significant up-regulation but also due to its relation to sucrose metabolic pathways. Following this clue, the enzymes and genes related to sucrose metabolism pathway were tested on blueberry fruits after 4OL fumigation. The result showed that sucrose content 28 % improved, the sucrose synthesis genes expression up-regulated and enzymes activity increased, the sucrose degradation genes expression down-regulated and enzymes activity suppressed. Meanwhile, <em>VcGLU3</em> associated with cell wall degradation was observed significantly down-regulated in transcriptomics. Moreover, the enzymes and genes related to cell wall degradation were verified and blueberry firmness were checked. The result indicated that firmness maintained well and the cell wall degradation genes expression down-regulated and enzymes activity suppressed. It indicated that 4OL could be a novel preservative in quality enhancement and retardation blueberry postharvest softening.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113273"},"PeriodicalIF":6.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-23DOI: 10.1016/j.postharvbio.2024.113257
Xuejin Li , Shenghu Luo , Jianduo Shen , Chaozhe Li , Wuernisha Kadeer , Lan Chen , Xihong Li , Yuqian Jiang , Yao Tang
This study investigates the regulatory mechanisms through which short-term high oxygen pre-stimulation (SHOP), combined with supercooled (SC) storage, synergistically enhances the anti-browning and cold tolerance of fresh-cut potatoes. Analyzed via LC-MS-based metabolomics and physiological assessments, the browning of fresh-cut potatoes involves polyphenol biosynthesis and membrane lipids oxidation. SHOP treatment enhanced amino acid metabolism in potato tubers and increased the activities of PPO, PAL, and POD by 63.8 %, 160.73 %, and 13.57 % compared with UT treatment, thereby inducing systemic resistance to cutting injuries and cold stress on day 0. However, the potato slices in SHOP-SC group showed suppressed PPO activity and reduced accumulation of browning associated phenolic compounds (especially chlorogenic acid and catechins) on day 4 compared with UT-SC group. Furthermore, SHOP improved membrane fluidity and reduced lipid peroxidation during SC storage, preserving membrane integrity in fresh-cut potatoes. Moreover, SHOP mitigated the stress response of fresh-cut potatoes and maintained a stable and less active physiological state during SC storage. This study provides novel insight into the molecular mechanisms underlying the synergistic browning inhibition and cold tolerance improvement in fresh-cut potatoes achieved by SHOP and SC storage, offering a promising strategy for shelf-life extension.
{"title":"Synergistic anti-browning effects of short-term high oxygen pre-stimulation and supercooled storage on fresh-cut potatoes by regulating polyphenol biosynthesis and membrane lipid oxidation","authors":"Xuejin Li , Shenghu Luo , Jianduo Shen , Chaozhe Li , Wuernisha Kadeer , Lan Chen , Xihong Li , Yuqian Jiang , Yao Tang","doi":"10.1016/j.postharvbio.2024.113257","DOIUrl":"10.1016/j.postharvbio.2024.113257","url":null,"abstract":"<div><div>This study investigates the regulatory mechanisms through which short-term high oxygen pre-stimulation (SHOP), combined with supercooled (SC) storage, synergistically enhances the anti-browning and cold tolerance of fresh-cut potatoes. Analyzed via LC-MS-based metabolomics and physiological assessments, the browning of fresh-cut potatoes involves polyphenol biosynthesis and membrane lipids oxidation. SHOP treatment enhanced amino acid metabolism in potato tubers and increased the activities of PPO, PAL, and POD by 63.8 %, 160.73 %, and 13.57 % compared with UT treatment, thereby inducing systemic resistance to cutting injuries and cold stress on day 0. However, the potato slices in SHOP-SC group showed suppressed PPO activity and reduced accumulation of browning associated phenolic compounds (especially chlorogenic acid and catechins) on day 4 compared with UT-SC group. Furthermore, SHOP improved membrane fluidity and reduced lipid peroxidation during SC storage, preserving membrane integrity in fresh-cut potatoes. Moreover, SHOP mitigated the stress response of fresh-cut potatoes and maintained a stable and less active physiological state during SC storage. This study provides novel insight into the molecular mechanisms underlying the synergistic browning inhibition and cold tolerance improvement in fresh-cut potatoes achieved by SHOP and SC storage, offering a promising strategy for shelf-life extension.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113257"},"PeriodicalIF":6.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-23DOI: 10.1016/j.postharvbio.2024.113272
Biao Wang , Jianmin Yun , Weihong Guo , Jiawei Shen , Ting Wang , Fengyun Zhao , Yuling Qu , Liang Yao
Browning and softening are typical characteristics of the postharvest quality degradation of button mushrooms. In this paper, comparative transcriptome analyses of mushroom samples during storage have been established based on sensory evaluations and physiological and biochemical indices analyses used to investigate the delaying effects of ozone fumigation combined with polyethylene nanopackaging (OCNP) on the browning and softening process, as well as their potential regulatory mechanisms. Our results indicated that the OCNP treatment reduced the respiratory rate of Agaricus bisporus, decreased the browning index and electrolyte leakage rate, and maintained the whiteness and firmness of postharvest mushrooms. In addition, OCNP inhibited the activities of browning-related enzymes (polyphenol oxidase and tyrosinase) and cell wall degrading enzymes (chitinase and β-1,3-glucanase) in the fruiting bodies, which delayed softening, while attenuating browning in A. bisporus. Transcriptomics analyses have revealed that OCNP inhibited the occurrence of browning by down-regulating genes involved in the antioxidant system (catalase and superoxide dismutase) as well as those related to browning enzymes (polyphenol oxidase and tyrosinase). Furthermore, OCNP also maintained the integrity of the cell walls by down-regulating genes associated with the degradation of cell wall components, such as chitin and β-1,3-glucan, thereby postponing softening. In summary, OCNP delayed the postharvest browning and softening of mushrooms by inhibiting the activity of browning-associated enzymes and mitigating the loss of cell wall components.
{"title":"Transcriptomic analysis reveals the mechanism of ozone fumigation combined with polyethylene nanopackaging for delaying the browning and softening of mushrooms (Agaricus bisporus)","authors":"Biao Wang , Jianmin Yun , Weihong Guo , Jiawei Shen , Ting Wang , Fengyun Zhao , Yuling Qu , Liang Yao","doi":"10.1016/j.postharvbio.2024.113272","DOIUrl":"10.1016/j.postharvbio.2024.113272","url":null,"abstract":"<div><div>Browning and softening are typical characteristics of the postharvest quality degradation of button mushrooms. In this paper, comparative transcriptome analyses of mushroom samples during storage have been established based on sensory evaluations and physiological and biochemical indices analyses used to investigate the delaying effects of ozone fumigation combined with polyethylene nanopackaging (OCNP) on the browning and softening process, as well as their potential regulatory mechanisms. Our results indicated that the OCNP treatment reduced the respiratory rate of <em>Agaricus bisporus</em>, decreased the browning index and electrolyte leakage rate, and maintained the whiteness and firmness of postharvest mushrooms. In addition, OCNP inhibited the activities of browning-related enzymes (polyphenol oxidase and tyrosinase) and cell wall degrading enzymes (chitinase and β-1,3-glucanase) in the fruiting bodies, which delayed softening, while attenuating browning in <em>A. bisporus</em>. Transcriptomics analyses have revealed that OCNP inhibited the occurrence of browning by down-regulating genes involved in the antioxidant system (catalase and superoxide dismutase) as well as those related to browning enzymes (polyphenol oxidase and tyrosinase). Furthermore, OCNP also maintained the integrity of the cell walls by down-regulating genes associated with the degradation of cell wall components, such as chitin and β-1,3-glucan, thereby postponing softening. In summary, OCNP delayed the postharvest browning and softening of mushrooms by inhibiting the activity of browning-associated enzymes and mitigating the loss of cell wall components.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113272"},"PeriodicalIF":6.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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