Postharvest Biology and Technology最新文献_第2页

Calmodulin-like protein CpCML46 interacts with transcription factor CpERF12 to regulate papaya fruit ripening 钙调素样蛋白CpCML46与转录因子CpERF12相互作用调控木瓜果实成熟

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-03 DOI: 10.1016/j.postharvbio.2025.114146

Qiunan Zhu , Xinmiao Kang , Keyuan Zhang , Faiz Ur Rahman , Xiangting Wang , Yankai Huang , Ruyi Luo , Hongtao Lei , Weixin Chen , Xueping Li , Xiaoyang Zhu

Calmodulin-like protein (CML) is one of calcium sensor proteins that are responsible for receiving, translating and transmitting calcium signaling to downstream targets. In this research, it was observed that CpCML46 exhibits an expression profile strongly associated with papaya fruit ripening. The transient overexpression and virus-induced gene silencing (VIGS) assays demonstrated that CpCML46 acts as a positive player in enhancing papaya fruit ripening. The heterologous overexpression of CpCML46 in tomato also promotes the ripening of fruits and promotes the transcription of ripening-associated genes. CpCML46 interacts with the transcription factor CpERF12, which acts as a transcriptional suppressor, with its expression level progressively declining during fruit ripening. Ethephon (ETH) treatment inhibits its expression while 1-methylcyclopropene (1-MCP) treatment promotes it. CpERF12 binds to and inhibits the promoter activities of the ethylene signal transduction-related gene CpETR2-like, and the cell wall degradation and remodeling-related genes CpPE, CpPE12 and CpEXPA11. The interaction of CpCML46 and CpERF12 reduced the inhibitory effect of CpERF12 on downstream target genes. Transient overexpression of CpERF12 inhibited the ripening process of papaya fruits, repressed the expression of CpETR2-like, CpPE, CpPE12 and CpEXPA11. The present work reveals that CpCML46 not function as canonical calcium sensor, but interact with CpERF12 to form a CpCML46-CpERF12 regulator module, involved in the ethylene signal to mediate papaya fruit ripening.

钙调素样蛋白（Calmodulin-like protein， CML）是一种钙传感器蛋白，负责钙信号的接收、翻译和传递。在本研究中，我们观察到CpCML46的表达谱与木瓜果实成熟密切相关。瞬时过表达和病毒诱导基因沉默（VIGS）实验表明，CpCML46在促进木瓜果实成熟中起积极作用。CpCML46在番茄中的异源过表达也促进了果实的成熟，促进了成熟相关基因的转录。CpCML46与转录抑制因子CpERF12相互作用，其表达水平在果实成熟过程中逐渐下降。乙烯利（ETH）抑制其表达，而1-甲基环丙烯（1-MCP）促进其表达。CpERF12结合并抑制乙烯信号转导相关基因CpETR2-like和细胞壁降解和重塑相关基因CpPE、CpPE12和CpEXPA11的启动子活性。CpCML46和CpERF12的相互作用降低了CpERF12对下游靶基因的抑制作用。CpERF12的过表达抑制了木瓜果实的成熟过程，抑制了cpetr2样蛋白、CpPE、CpPE12和CpEXPA11的表达。本研究表明，CpCML46不是典型的钙传感器，而是与CpERF12相互作用形成CpCML46-CpERF12调控模块，参与乙烯信号介导木瓜果实成熟。

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引用次数: 0

Fusaricidins from Paenibacillus polymyxa ZJU11 mediate the biological control of postharvest mango anthracnose 多粘类芽孢杆菌ZJU11的镰刀菌素介导芒果采后炭疽病的生物防治

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2026-01-02 DOI: 10.1016/j.postharvbio.2025.114148

Cui Sun , Yihan Wang , Yihu Pi , Jinping Cao , Yue Wang , Chaoyi Hu , Chongde Sun

Mango fruits are highly susceptible to infection by Colletotrichum species, leading to the severe economic losses. In this study, an antagonist of Paenibacillus polymyxa ZJU11 with strong biocontrol activity was identified and its antifungal mechanisms were investigated. P. polymyxa ZJU11 effectively suppressed the growth of pathogens of mango fruit in vitro and in vivo. The whole genome sequencing and LC-MS/MS results revealed that the active component in P. polymyxa ZJU11 exerting antifungal activity was Fusaricidin A, B, C and D. The cell membrane integrity of pathogens was demonstrated to be damaged after Fusaricidin extract treatment by PI staining, SEM and TEM observation. Additionally, transcriptomic analysis indicated Fusaricidin extract upregulated the gene expression in cell cycle, MAPK signaling pathway, and autophagy in C. asianum HIN6, induced cell death. This study provides a promising and suitable biocontrol agent to protect the postharvest mango fruit from fungal infection.

芒果果实极易受到炭疽病菌的侵染，造成严重的经济损失。本研究鉴定了一种具有较强生物防治活性的多粘类芽孢杆菌拮抗剂ZJU11，并对其抑菌机制进行了探讨。P. polymyxa ZJU11在体内外均能有效抑制芒果果实病原菌的生长。全基因组测序和LC-MS/MS结果显示，多粘菌ZJU11中发挥抗真菌活性的活性成分为Fusaricidin A、B、C和d。PI染色、SEM和TEM观察表明，Fusaricidin提取物处理后，病原菌细胞膜完整性被破坏。此外，转录组学分析表明，Fusaricidin提取物上调亚洲镰刀菌HIN6细胞周期、MAPK信号通路和自噬基因的表达，诱导细胞死亡。本研究为芒果果实采后真菌侵染提供了一种有前景的生物防治剂。

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引用次数: 0

Gibberellin delays the red-turning of jujube fruit during storage via browning inhibition: Insights from targeted metabolomics 赤霉素通过褐变抑制延缓红枣果实在储存期间的变红：来自目标代谢组学的见解

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-12-30 DOI: 10.1016/j.postharvbio.2025.114147

Tianyu Li , Jiaqi Liu , Jialin Tao , Tong Wu , Mengjun Liu , Shoukun Han

Postharvest red-turning critically impacts jujube fruit quality and shelf life. This study demonstrated that gibberellin acid (GA₃) treatment significantly delayed jujube fruit red-turning during storage, preserving the contents of anthocyanins, carotenoids, and chlorophylls in severe red-turning pericarp. Integrated metabolomic and biochemical analyses revealed decreased anthocyanins and flavonoids but enriched phenolic acids and lipids during red-turning, distinguishing the postharvest red-turning from preharvest coloration of jujube fruit. Furthermore, we also found that GA₃ delayed red-turning by maintaining phenolic and flavonoid levels and inhibiting the accumulation of phenolic acids and lipids while suppressing lignin synthesis and reactive oxygen species (ROS) accumulation. Crucially, GA₃ concurrently inhibited polyphenol oxidase (PPO), laccase (LAC), and peroxidase (POD) activities and downregulated key genes (including ZjPPO1, ZjPPO2, ZjLAC7, and ZjPOD1), confirming the key role of pericarp browning in red-turning. Correlation analysis further elucidated the close connections between red-turning and the activities of PPO and POD, levels of ROS and lignin, as well as the content of phenolic compounds. These findings elucidate GA₃-regulated red-turning mechanisms in postharvest jujube fruit and provide theoretical foundations for innovative jujube storage technologies.

采后变红对红枣果实品质和保质期有重要影响。本研究表明，赤霉素酸（GA3）处理显著延缓了红枣果实在贮藏过程中的红化，保留了严重红化果皮中花青素、类胡萝卜素和叶绿素的含量。综合代谢组学和生化分析表明，在红枣果实变红过程中，花青素和类黄酮含量降低，酚酸和脂质含量增加，这是红枣果实采后变红与采前变红的区别。此外，我们还发现GA3通过维持酚类和类黄酮水平，抑制酚酸和脂质的积累，同时抑制木质素合成和活性氧（ROS）积累来延缓变红。关键是，GA3同时抑制多酚氧化酶（PPO）、漆酶（LAC）和过氧化物酶（POD）活性，下调关键基因（包括ZjPPO1、ZjPPO2、ZjLAC7和ZjPOD1），证实了果皮褐变在变红过程中的关键作用。相关分析进一步阐明了变红与PPO和POD活性、ROS和木质素水平以及酚类化合物含量之间的密切关系。这些研究结果阐明了ga3调控枣果采后变红的机理，为枣果保鲜技术的创新提供了理论基础。

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引用次数: 0

Molecular and metabolic response of ‘Piccolo’ cherry tomato to Graduated Controlled Atmosphere ‘短笛’樱桃番茄对分级可控气氛的分子和代谢响应

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-12-29 DOI: 10.1016/j.postharvbio.2025.114133

Natalia Falagán, Roberta Tosetti, Ewan Gage, Leon A. Terry

Controlled atmosphere (CA) is used to extend the postharvest life of climacteric fruit by suppressing respiration and delaying ethylene mediated ripening. However, sudden exposure to low oxygen conditions can induce hypoxic stress, triggering metabolic and hormonal disruptions that negatively impact fruit quality. This study aimed to understand the mechanisms underlying ethylene sensitivity under low oxygen conditions through the application of a novel approach to CA, called Graduated Controlled Atmosphere (GCA), in ‘Piccolo’ cherry tomato. Compared to standard CA, GCA treatment resulted in greater suppression of respiration and improved firmness retention, indicating reduced physiological stress and slower cell wall degradation. Gene expression analysis revealed downregulation of NCED1, ACS, and ACO genes under GCA, indicating delayed ethylene-associated transcriptional activity. These hormonal adjustments were also reflected in lower abscisic acid (ABA) concentrations, implying a more stable ripening trajectory. Besides hormonal modulation, GCA-treated fruit exhibited alterations in primary metabolism. Sucrose accumulation and changes in malate levels under GCA conditions suggest a shift in energy metabolism, consistent with improved hypoxia tolerance. However, a notable trade-off was observed in reduced lycopene accumulation, potentially due to lower oxidative signalling and shared precursors between carotenoid and ABA biosynthesis. These findings demonstrate that GCA promotes a more controlled physiological and molecular response to hypoxic storage by reducing stress-associated metabolic and hormonal activity. GCA, as an advanced postharvest strategy, enhances texture retention and may reduce quality losses during storage. This work provides new mechanistic insights into hypoxia adaptation in fruit and supports the use of gradual atmosphere modification to optimise CA protocols.

控制气氛（CA）通过抑制呼吸作用和延迟乙烯介导的成熟来延长更年期果实的采后寿命。然而，突然暴露在低氧条件下会引起缺氧应激，引发代谢和激素紊乱，对水果品质产生负面影响。本研究旨在了解低氧条件下乙烯敏感性的机制，通过应用一种新的CA方法，称为渐次可控气氛（GCA），在‘ Piccolo ’樱桃番茄中。与标准CA相比，GCA治疗导致更大的呼吸抑制和硬度保持改善，表明生理应激减少，细胞壁降解减慢。基因表达分析显示，GCA下NCED1、ACS和ACO基因下调，表明乙烯相关转录活性延迟。这些激素调节也反映在较低的脱落酸（ABA）浓度上，这意味着更稳定的成熟轨迹。除了激素调节外，gca处理的果实还表现出初级代谢的改变。蔗糖积累和苹果酸水平在GCA条件下的变化表明能量代谢的转变，与缺氧耐受性的提高一致。然而，在减少番茄红素积累中观察到一个显著的权衡，可能是由于氧化信号的降低和类胡萝卜素和ABA生物合成之间共享的前体。这些发现表明，GCA通过降低与应激相关的代谢和激素活性，促进了对缺氧储存的更可控的生理和分子反应。GCA作为一种先进的采后策略，可以提高果实的质地保留，减少贮藏过程中的品质损失。这项工作为水果的缺氧适应提供了新的机制见解，并支持使用渐进的大气调节来优化CA协议。

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引用次数: 0

Bioprotective potential of Bacillus spp. against postharvest pathogens of Khasi Mandarin (Citrus reticulata Blanco) with enhanced shelf life 芽孢杆菌对延长保质期的卡西柑采后病原菌的生物保护潜力

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-12-24 DOI: 10.1016/j.postharvbio.2025.114142

Trishna Taye , Popy Bora

The global citrus industry faces significant annual economic losses due to postharvest decay pathogens. This study focused on identifying the major postharvest pathogens affecting Khasi Mandarin (Citrus reticulata Blanco) and evaluating the efficacy of Bacillus spp. as biocontrol agents, as well as their impact on fruit quality. Based on morpho-cultural characteristics, the isolated pathogens were identified as Penicillium digitatum (PQ606361) and Aspergillus niger (PV657140). Four Bacillus spp. Cell-free supernatants (CFS) were screened against these pathogens, all of which significantly inhibited fungal mycelial growth. Among them, Bacillus subtilis LB22 CFS exhibited the highest inhibition, at 74.60 % for P. digitatum and 86.20 % for A. niger. Scanning Electron Microscopy (SEM) revealed morphological disintegration in fungal hyphae and spores upon treatment with B. subtilis LB22 CFS. GC-MS analysis of the LB22 CFS identified nine antimicrobial and four ethylene-inhibiting compounds, including 4-di-tert-butylphenol, n-hexadecanoic acid, and 1H-indene derivatives. In-vivo assays of CFS demonstrated that fruit coating with the B. subtilis CFS prevented A. niger and P. digitatum invasion in khasi mandarin fruits with 100 % bioprotective efficiency upto 15 days against 100 % fruit decay in control at 3 days. Quality assessment of treated fruits after 15 days of storage revealed a total soluble solids (TSS) value of 11.90°Brix and titratable acidity of 0.85 %, both statistically non-significant compared to the control. Organoleptic evaluations, based on colour, flavour, texture, taste, and overall acceptability, supported the efficacy of the treatment. Collectively, our findings confirm that B. subtilis LB22 acts as a potent biocontrol agent against green and black mold in both in vitro and in vivo conditions. Our study hence perch Bacillus subtilis LB22 CFS based fruit coating as a promising option for post harvest handling of Khasi mandarin fruits, preventing fruit decay and enhancing the shelf life.

由于采后腐烂病原体，全球柑橘产业面临着重大的年度经济损失。本研究旨在鉴定影响卡西柑采后的主要病原菌，并评价芽孢杆菌（Bacillus spp.）作为生物防治剂的效果及其对果实品质的影响。根据形态培养特征，分离的病原菌鉴定为指状青霉（PQ606361）和黑曲霉（PV657140）。筛选出4种芽孢杆菌无细胞上清液（CFS），均能显著抑制真菌菌丝生长。其中，枯草芽孢杆菌LB22 CFS对digitatum和niger的抑制率分别为74.60 %和86.20 %。扫描电镜（SEM）显示枯草芽孢杆菌LB22 CFS对真菌菌丝和孢子的形态学破坏。GC-MS分析鉴定出9种抗菌化合物和4种乙烯抑制化合物，包括4-二叔丁基酚、正十六酸和1h -独立衍生物。CFS的体内试验表明，用枯草芽孢杆菌CFS包衣可有效防止黑曲霉和指状线虫对卡西柑果实的侵袭，15天内的生物保护效率为100% %，3天内的果实腐烂率为100% %。贮藏15天后的果实质量评价显示，总可溶性固形物（TSS）值为11.90°Brix，可滴定酸度为0.85 %，与对照相比均无统计学意义。基于颜色、风味、质地、味道和总体可接受性的感官评价支持了治疗的有效性。总之，我们的研究结果证实，枯草芽孢杆菌LB22在体外和体内条件下都是一种有效的生物防治剂，可以对抗绿霉和黑霉。本研究认为，枯草芽孢杆菌LB22 CFS为基材的覆膜剂是一种很有前途的覆膜剂，可用于卡西柑采后处理，防止果实腐烂，延长果实保质期。

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引用次数: 0

Postharvest application of glutathione and selenium and their combination enhances quality performance of chestnut 采后施用谷胱甘肽和硒及其组合可提高板栗品质性能

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-12-24 DOI: 10.1016/j.postharvbio.2025.114135

Nannan Dong , Ling Wei , Wanfeng Wu , Yizhou Xiang , Qiannan Hu , Yaoqi Zhang , Zisheng Luo , Yunbin Jiang , Li Li

Fresh chestnut is highly susceptible to postharvest browning and quality deterioration. Therein, the effect of postharvest application of exogenous sodium selenite (Na₂SeO₃) and glutathione (GSH) and their combination on quality attributes of fresh chestnut during shelf-life was investigated in present study. Compared with those in the control group, chestnuts treated with Na₂SeO₃ and GSH on day 10 presented an increase in firmness of 90.9 % and a reduction in weight loss and browning index of 44.4 % and 5.1 %, respectively. The combination of Na₂SeO₃ and GSH enhanced nutritional quality and antioxidant performance by increasing the ascorbic acid (AsA) and GSH content and increasing the expression levels of CmSOD and CmCAT on day 20. The inorganic selenium (Se) in the GSH+Na₂SeO₃ group was predominantly selenite on day 0. After 20 days, inorganic Se was metabolized into organic Se (selenocysteine, methylselenocysteine, and selenomethionine). Our results indicated that the combination of Na₂SeO₃ and GSH enhanced antioxidant properties, promoted the accumulation of AsA and GSH, facilitated Se accumulation and biotransformation, and extended the shelf-life of chestnuts. Overall, this work provided a promising strategy to improve the postharvest storage quality and nutritional values of fresh chestnut.

新鲜栗子采后极易褐变和品质变差。为此，本研究以采后施用外源亚硒酸钠（Na2SeO3）和谷胱甘肽（GSH）及其组合对鲜板栗贮藏期品质性状的影响为研究对象。与对照组相比，Na2SeO3和GSH处理第10天栗子的硬度增加了90.9% %，失重和褐变指数分别降低了44.4% %和5. %。Na2SeO3和GSH在第20天通过提高抗坏血酸（AsA）和GSH含量以及CmSOD和CmCAT的表达水平，提高了营养品质和抗氧化性能。第0天，GSH+Na2SeO3组的无机硒（Se）以亚硒酸盐为主。20 d后，无机硒代谢为有机硒（硒半胱氨酸、甲基硒半胱氨酸和硒代蛋氨酸）。结果表明，Na2SeO3与GSH的结合可提高板栗的抗氧化性能，促进AsA和GSH的积累，促进Se的积累和生物转化，延长板栗的货架期。本研究为提高鲜板栗采后贮藏品质和营养价值提供了一种有前景的策略。

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引用次数: 0

Green synthesis of silver nanoparticles by endophytic Burkholderia plantarii and their protection efficacy against postharvest tomato soft rot 植物内生伯克霍尔德菌绿色合成纳米银及其对番茄采后软腐病的防护效果

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-12-23 DOI: 10.1016/j.postharvbio.2025.114141

Kangwei Xie , Yusha Du , Jiatong Zhang , Xiujuan Gan , Tieyi Hu , Niqi Xie , Xingyong Yang

Tomatoes, a vital cash crop, are highly susceptible to postharvest soft rot caused by Pectobacterium carotovorum. Bio-synthesized silver nanoparticles (AgNPs), generated via a green synthesis approach, offer a promising strategy for managing postharvest diseases due to their potent antibacterial properties. In this study, the endophytic Burkholderia plantarii BpMS90 was shown to reduce silver ions to form AgNPs (Bp-AgNPs). These nanoparticles exhibited characteristic surface plasmon resonance peaks between 400–450 nm and had an average size of 78.04 ± 29.66 nm. In vitro assays revealed that Bp-AgNPs had a minimum inhibitory concentration of 0.625 ppm against P. carotovorum and demonstrated broad-spectrum antibacterial activity against six additional bacterial species. Microstructural analysis indicated that Bp-AgNPs inhibited P. carotovorum proliferation by compromising cellular integrity. Treatment with Bp-AgNPs significantly decreased the incidence of tomato soft rot and minimized postharvest weight loss. Additionally, 16S rRNA gene sequencing demonstrated that Bp-AgNPs preserved the relative diversity of endophytic bacterial communities in postharvest tomatoes, notably sustaining the relative abundance of Weissella species in comparison to diseased tomatoes. Metabolomic correlation analysis revealed that Weissella abundance was positively associated with L-phenylalanine, citric acid, and L-tryptophan levels, metabolites implicated in postharvest disease resistance. These findings highlight the potential of biosynthetic Bp-AgNPs as effective antimicrobial agents and support their application in sustainable postharvest disease management.

西红柿是一种重要的经济作物，采收后极易受到由胡萝卜乳杆菌引起的软腐病的影响。生物合成银纳米颗粒（AgNPs）通过绿色合成方法产生，由于其强大的抗菌特性，为管理采后疾病提供了一种有希望的策略。在这项研究中，植物内生伯克霍尔德菌BpMS90被证明可以还原银离子形成AgNPs （Bp-AgNPs）。这些纳米粒子表面等离子体共振峰在400-450 nm之间，平均尺寸为78.04±29.66 nm。体外实验表明，Bp-AgNPs对胡萝卜假单胞菌的最低抑菌浓度为0.625 ppm，对另外6种细菌具有广谱抑菌活性。微观结构分析表明，Bp-AgNPs通过破坏细胞完整性来抑制胡萝卜芽孢杆菌的增殖。Bp-AgNPs处理显著降低了番茄软腐病的发病率，最大限度地减少了采后失重。此外，16S rRNA基因测序表明，Bp-AgNPs保留了采后番茄内生细菌群落的相对多样性，特别是与患病番茄相比，保持了Weissella物种的相对丰度。代谢组学相关分析显示，Weissella丰度与l -苯丙氨酸、柠檬酸和l -色氨酸水平呈正相关，这些代谢物与采后抗病性有关。这些发现突出了生物合成Bp-AgNPs作为有效抗菌剂的潜力，并支持其在可持续采后疾病管理中的应用。

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引用次数: 0

Dual-mechanism biocontrol against postharvest soft rot by Bacillus amyloliquefaciens BacXF14 via pathogen inhibition and microbiome modulation 解淀粉芽孢杆菌BacXF14对采后软腐病病原菌抑制和微生物组调控的双机制生物防治

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-12-23 DOI: 10.1016/j.postharvbio.2025.114137

Bingce Wang , Xia Lei , Hao Luo , Xiaolan Yang , Xianhui Yin , Weizhen Wang , Youhua Long

Kiwifruit, a climacteric fruit, rapidly ripens and is highly susceptible to postharvest deterioration, and Botryosphaeria dothidea is the most important pathogen. This vulnerability highlights the urgent need for safe and effective preservation strategies. In this study, a biocontrol bacterium identified as Bacillus amyloliquefaciens BacXF14, which harbors 8 known biosynthesis-related gene clusters for secondary metabolites, was isolated from the surface of healthy kiwifruit. Both BacXF14 and its cell-free supernatant (CFS) significantly suppressed the mycelial growth and biomass accumulation of six major postharvest pathogens, exhibiting particularly strong antagonistic activity against B. dothidea. Further analyses revealed that the CFS induced spore deformation and inhibited the germination of both spores and sclerotia in B. dothidea. It also severely disrupted the hyphal cell wall and membrane integrity and suppressed the activities of the infection-related hydrolytic enzymes β-glucosidase and pectinase. LC–MS/MS analysis confirmed the presence of 26 structurally diverse antifungal compounds in the supernatant. BacXF14 stably colonized kiwifruit surfaces for at least 15 days, significantly reducing the incidence of soft rot. In addition to direct antagonism, it modulates the epiphytic microbiome by suppressing pathogenic fungi and enriching beneficial taxa. This microbial shift improved fruit quality in B. dothidea-infected kiwifruit and delayed ripening in healthy fruit. These findings highlight the dual roles of BacXF14 in both pathogen inhibition and microbiome modulation, offering a promising and sustainable strategy for postharvest disease control and shelf-life extension in kiwifruit.

猕猴桃是一种更年期水果，成熟快，采后易变质，其中最主要的病原菌为葡萄球菌。这一脆弱性凸显了对安全有效的保护策略的迫切需要。本研究从健康猕猴桃表面分离到一株已知含有8个次生代谢产物生物合成相关基因簇的解淀粉芽孢杆菌BacXF14。BacXF14及其无细胞上清液（CFS）均能显著抑制6种主要采后病原菌的菌丝生长和生物量积累，对白僵菌表现出特别强的拮抗活性。进一步分析表明，CFS诱导孢子变形，抑制孢子和菌核的萌发。严重破坏菌丝细胞壁和膜的完整性，抑制与感染相关的水解酶β-葡萄糖苷酶和果胶酶的活性。LC-MS /MS分析证实，上清液中存在26种结构多样的抗真菌化合物。BacXF14在猕猴桃表面稳定定殖至少15天，显著降低了软腐病的发病率。除了直接拮抗外，它还通过抑制病原菌和丰富有益类群来调节附生微生物群。这种微生物的转移改善了双歧杆菌感染猕猴桃的果实品质，延缓了健康果实的成熟。这些发现强调了BacXF14在病原菌抑制和微生物组调节中的双重作用，为猕猴桃采后病害控制和延长货架期提供了一种有前景的可持续策略。

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引用次数: 0

Non-destructive monitoring of postharvest quality changes in chili (Capsicum frutescens) during storage using image processing coupled with machine learning 使用图像处理与机器学习相结合的方法对辣椒（Capsicum frutescens）在储存期间的采后质量变化进行无损监测

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-12-22 DOI: 10.1016/j.postharvbio.2025.114140

Wong Junyang , Mohamad Yusof Maskat , Maimunah Mohd Ali

Chili (Capsicum frutescens) is an important vegetable crop widely cultivated in tropical and subtropical regions, where postharvest quality deterioration particularly color and freshness loss, poses significant challenges during storage. Traditional visual inspection methods are often subjective and inconsistent. This study evaluated the postharvest quality of bird’s eye chilies stored at 5 °C and 28 °C on Days 1, 3, and 5 using non-destructive image processing combined with machine learning approach. Image features were extracted from chili samples and classified using Linear Discriminant Analysis (LDA), Support Vector Machine (SVM), k-Nearest Neighbors (k-NN), and ensemble model. Using a 70:30 training–testing split, SVM achieved the highest average classification accuracy (69.1 % at 5 °C and 66.7 % at 28 °C). Under 10-fold stratified cross-validation, k-NN and ensemble models demonstrated improved robustness, with the ensemble yielding the highest average classification accuracy (74.08 % at 5 °C and 79.26 % at 28 °C). To complement the machine learning models, deep learning models where a feature-level 1D-Convolutional Neural Network was implemented, achieving an average 57.6 % accuracy under 10-fold, while an image-based MobileNetV2 architecture achieved 78.8 % validation accuracy. These results highlighted that automatic feature extraction can outperform hand-crafted features for postharvest chili quality assessment. Additionally, Partial Least Squares (PLS) regression identified the L* value as the most effective color parameter for quality detection (R² > 0.93). Overall, integrating image processing with machine learning and supported by deep learning offers a reliable, non-invasive framework for evaluating postharvest chili quality.

辣椒（Capsicum frutescens）是一种广泛种植于热带和亚热带地区的重要蔬菜作物，其采后品质劣化，尤其是色泽和新鲜度的丧失，给贮藏带来了重大挑战。传统的目视检查方法往往是主观的和不一致的。本研究利用非破坏性图像处理结合机器学习方法，评估了在5°C和28°C条件下储存的鸟眼辣椒在第1、3和5天的采后质量。利用线性判别分析（LDA）、支持向量机（SVM）、k-最近邻（k-NN）和集成模型对辣椒样本的图像特征进行分类。使用70:30的训练-测试分割，SVM获得了最高的平均分类准确率（5°C时为69.1 %，28°C时为66.7 %）。在10倍分层交叉验证下，k-NN和集成模型显示出更好的鲁棒性，其中集成的平均分类准确率最高（5°C时为74.08 %，28°C时为79.26 %）。为了补充机器学习模型，实现了特征级1d -卷积神经网络的深度学习模型，在10倍下实现了平均57.6 %的准确率，而基于图像的MobileNetV2架构实现了78.8 %的验证准确率。这些结果表明，在采后辣椒品质评估中，自动特征提取优于手工特征提取。此外，偏最小二乘（PLS）回归发现L*值是质量检测最有效的颜色参数（R²> 0.93）。总的来说，将图像处理与机器学习结合起来，并在深度学习的支持下，为评估采后辣椒的质量提供了一个可靠的、非侵入性的框架。

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引用次数: 0

Antifungal efficacy and mechanisms of slightly acidic electrolyzed water against Trichothecium roseum 微酸性电解水对玫瑰曲霉菌的抑菌效果及机制研究

IF 6.8 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-12-22 DOI: 10.1016/j.postharvbio.2025.114139

Chenyang Li , Jun Ling , Yue Han , Xiaobing Li , Xueqiang Chai , Huali Hu , Hongsheng Zhou , Xuesong Liu , Chun Zhao , Yingtong Zhang , Pengxia Li

Pink mold caused by Trichothecium roseum (T. roseum) is a major postharvest disease affecting fruit and vegetable crops, necessitating eco-friendly alternatives to chemical fungicides for its control. This study investigates the antifungal efficacy of Slightly Acidic Electrolyzed Water (SAEW) against T. roseum in vitro and in vivo. SAEW can inhibit the mycelial growth and spore germination of T. roseum, as well as significantly reduce disease development on apples and tomatoes in a dose-dependent manner, with complete inhibition achieved at ACC values of 100 mg L⁻¹. SAEW disrupted fungal membrane integrity, as indicated by propidium iodide staining, increased electrolyte leakage, reduced total lipid and ergosterol content, and elevated malondialdehyde (MDA) levels. Additionally, SAEW treatment downregulated the expression and enzyme activities of several cell wall-degrading enzymes associated with pathogenicity. These results collectively demonstrate that SAEW is highly effective in reducing postharvest diseases caused by T. roseum and has strong potential as a green alternative for fruit preservation.

玫瑰毛霉（Trichothecium roseum， T. roseum）引起的粉红霉菌是影响果蔬作物的主要采后病害，需要使用环保的化学杀菌剂来防治。本文研究了微酸性电解水（SAEW）对roseum的体外和体内抑菌作用。SAEW可以抑制玫瑰镰刀菌的菌丝生长和孢子萌发，并以剂量依赖的方式显著降低苹果和番茄的病害发展，ACC值为100 mg L−1时完全抑制。碘化丙啶染色表明，SAEW破坏了真菌膜的完整性，增加了电解质泄漏，降低了总脂质和麦角甾醇含量，升高了丙二醛（MDA）水平。此外，SAEW处理下调了几种与致病性相关的细胞壁降解酶的表达和酶活性。综上所述，SAEW可有效减少玫瑰红霉的采后病害，具有作为水果绿色保鲜的潜力。

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引用次数: 0