Postharvest Biology and Technology最新文献

{"title":"Volatile organic compounds from Meyerozyma guilliermondii JY19 trigger apoptosis-like cell death in Botrytis cinerea and control postharvest gray mold of ginseng berries","authors":"Guanzhong Ding ,&nbsp;Fan Zhou ,&nbsp;Peng Mu ,&nbsp;Lidan Zhang ,&nbsp;Yue Zhang ,&nbsp;Yidong Zheng ,&nbsp;Wenwen Jiang ,&nbsp;Ning Liu","doi":"10.1016/j.postharvbio.2026.114176","DOIUrl":"10.1016/j.postharvbio.2026.114176","url":null,"abstract":"<div><div>Gray mold caused by <em>Botrytis cinerea</em> severely compromises the postharvest value of ginseng berries. We assessed the endophytic yeast <em>Meyerozyma guilliermondii</em> JY19 as a green control and delineated a VOC-mediated mode of action. JY19 inhibited <em>B. cinerea in vitro</em> in diffusible-metabolite, cell-free supernatant, and sealed double-plate VOC assays. Scanning electron microscopy revealed hyphal surface collapse and fissures. VOC exposure induced reactive oxygen species and programmed cell death in conidia based on DCFH-DA staining, Annexin V–FITC/PI imaging, and flow cytometry. HS-SPME–GC–MS profiling identified a characteristic VOC repertoire; 3,5-diethyl-2-methylpyrazine, 4-methyl-2-pentanol, and trans-ocimenol showed dose-dependent inhibition with IC₅₀ values of 9.5, 23.1, and 26.7 μL L⁻¹ , respectively, whereas 1-hydroxy-2-propanone was inactive. RNA-seq showed concerted repression of nutrient-transport systems and xenobiotic detoxification (including multiple cytochrome P450s), together with attenuation of sphingolipid and glycosphingolipid pathways and MAPK signaling; oxidative phosphorylation was upregulated, consistent with metabolic compensation under VOC. <em>In vivo</em>, JY19 VOCs delayed disease onset and reduced lesion area by 79.3 %, 77.3 %, and 39.3 % on days 1, 2, and 3, respectively, thereby limiting decay under high humidity. These multi-level data support a mode-of-action model in which JY19 VOCs compromise membrane integrity and nutrient acquisition, trigger ROS-driven programmed cell death, and constrain fungal growth and virulence. To our knowledge, this is the first integrated <em>in vitro</em>, cytological, transcriptomic, and <em>in vivo</em> evaluation of VOC-based gray mold control on ginseng berries, positioning JY19 and its dominant VOCs as residue-lean biofumigation leads for postharvest management.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"235 ","pages":"Article 114176"},"PeriodicalIF":6.8,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A natural edible coating with enhanced antioxidant activity based on tara gum/Sa-son seed gum/sea buckthorn fruit oil incorporated with silk fibroin peptide for litchi and cherry preservation","authors":"Qianrun Li ,&nbsp;Yulong Chen ,&nbsp;Yunpeng Wang ,&nbsp;Yufei Zhou ,&nbsp;Chao-an Long","doi":"10.1016/j.postharvbio.2026.114185","DOIUrl":"10.1016/j.postharvbio.2026.114185","url":null,"abstract":"<div><div>In this study, natural, nontoxic silk fibroin peptide (SFP) was incorporated into blended coatings composed of tara gum (TG), Sa-son seed gum (SSG) and sea buckthorn fruit oil (SBFO), and the TSS-SBFO coating film properties were characterized. The results indicated that incorporating 0.15 % (w/v) SFP significantly increased the film’s elongation at break and UV-blocking properties, while also enhanced the oxygen and carbon dioxide barrier properties of the film. Furthermore, the addition of SFP effectively enhanced the antioxidant activity and affinity for fruit surfaces of the TS-SBFO coating. Subsequently, TS-SBFO and TSS₁₅-SBFO coatings were subsequently applied to litchis and cherries to assess their preservation effects. Results showed that both coating treatments maintained fruit quality during storage comparing to uncoated treatment, while coating with TSS₁₅-SBFO had a more prominent effect in preserving the levels of titratable acid and total soluble solids, and delaying their weight losses and respiratory intensity. In addition, the TSS₁₅-SBFO coating treatment increased the antioxidant capacity of litchis and cherries by inhibiting MDA content and increasing the activity of antioxidant enzymes. Therefore, as a safe and environmentally friendly novel edible coating film, the TSS₁₅-SBFO shows promising potential as an alternative to conventional petroleum-based packaging for extending the shelf-life of fruits.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"235 ","pages":"Article 114185"},"PeriodicalIF":6.8,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of WRKY transcription factors in Chimonanthus praecox var. concolor reveals the role of CpWRKY54 in winter flower senescence","authors":"Daofeng Liu ,&nbsp;Renwei Huang ,&nbsp;Qiong Hou ,&nbsp;Qing Yang ,&nbsp;Jiahui Zhao ,&nbsp;Xia Wang ,&nbsp;Zheng Li ,&nbsp;Mingyang Li ,&nbsp;Shunzhao Sui","doi":"10.1016/j.postharvbio.2026.114163","DOIUrl":"10.1016/j.postharvbio.2026.114163","url":null,"abstract":"<div><div><em>Chimonanthus praecox</em> is a traditional ornamental tree in China, flowering in winter at low temperature, which may have its unique molecular mechanism of flower opening and senescence. However, there is limited research on the molecular network regulatory mechanisms underlying the <em>C. praecox</em> flower senescence. Research showed that WRKY family is one of the largest transcription factors regulating senescence. In this study, 74 CpWRKY family members were identified in <em>C.praecox</em> var. <em>Concolor</em> genome. Based on the WRKY family gene profile during flower opening and senescence using the transcriptome database, the expression of 31 <em>CpWRKYs</em> was significantly induced during flower senescence. Among them, the expression of <em>CpWRKY54</em> was significantly affected by flower senescence and specifically induced by low temperature. Additionally, the inducibility of <em>CpWRKY54</em> promoter activity was consistent with the pattern of gene expression. Moreover, the overexpression of <em>CpWRKY54</em> in petunia significantly accelerated the flower senescence. The yeast two-hybrid and bimolecular fluorescence complementation showed the CpWRKY54 interacted with a MADS-box gene, AGAMOUS-LIKE 29 (<em>CpAGL29</em>), while the yeast one-hybrid. dual-luciferase reporter assays and electrophoretic mobility shift assay (EMSA) showed that CpAGL29 is an upstream regulatory protein of <em>CpWRKY42</em>. Overexpression of <em>CpWRKY42</em> also accelerated the flower senescence in transgenic petunia. The potential downstream genes, including autophagy- and senescence-associated genes containing W-box elements in their promoters that maybe related to flower senescence, and can be regulated by CpWRKY54 and CpWRKY42, were also identified from the genome. This transcription regulatory network provides new insights into the molecular mechanism underlying <em>C. praecox</em> flower longevity during winter.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"235 ","pages":"Article 114163"},"PeriodicalIF":6.8,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cinnamyl alcohol dehydrogenase orthologous pair CitCAD7 (CsCAD7/CgCAD6) promotes juice sac lignification during postharvest senescence in citrus fruit","authors":"Weiyan Lu ,&nbsp;Yaci Liu ,&nbsp;Qiulin Yang ,&nbsp;Kaifang Zeng ,&nbsp;Yunjie Zhang ,&nbsp;Chunlian Huang ,&nbsp;Shixiang Yao","doi":"10.1016/j.postharvbio.2026.114175","DOIUrl":"10.1016/j.postharvbio.2026.114175","url":null,"abstract":"<div><div>Granulation represents a senescence trajectory in citrus fruit, marked by lignification of juice sacs. Cinnamyl alcohol dehydrogenase (CAD) catalyzes the final reduction step in monolignol biosynthesis, yet the roles of individual CAD paralogues during citrus granulation remain unknown. Here, we performed a systematic analysis of the <em>CAD</em> gene family in sweet orange (<em>Citrus sinensis</em>) and pomelo (<em>Citrus grandis</em>) to probe their roles in juice sac lignification during postharvest senescence. We identified eight <em>CAD</em> genes in the <em>C. sinensis</em> genome and seven in the <em>C. grandis</em>. Across multiple cultivars and growing seasons, only one member—<em>CsCAD7</em> and its pomelo ortholog <em>CgCAD6</em>—showed consistent transcriptional upregulation that correlated with juice sac granulation. The two genes showed near-identical coding sequences and highly similar 2-kb promoter regions (95.10 % identity) with conserved cis-regulatory landscapes, including SNBE motifs recognized by NAC transcription factors; we therefore refer to this ortholog pair as <em>CitCAD7</em> (<em>CsCAD7</em>/<em>CgCAD6</em>). Transient overexpression of <em>CitCAD7</em> (<em>CsCAD7</em> allele) enhanced lignin accumulation across diverse systems, including <em>Nicotiana benthamiana</em> leaves, citrus leaves and pericarp, the juice sacs themselves, and strawberry fruit (<em>Fragaria × ananassa</em>). Moreover, dual-luciferase assays showed that the NAC transcription factor CitNSF1 activates the <em>CitCAD7</em> promoter <em>in vivo</em>. These results suggest <em>CitCAD7</em> as a granulation-responsive regulator linking CitNSF1 activation to the terminal step of monolignol formation. This work fills a key gap in the molecular framework of citrus granulation, reinforcing the “Disorder of cell wall metabolism” hypothesis and highlighting <em>CitCAD7</em> as a strategic genetic target for mitigating postharvest quality loss.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"235 ","pages":"Article 114175"},"PeriodicalIF":6.8,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond human vision: Highlighting and detecting inconspicuous defects in citrus fruits using UV-fluorescence imaging and YOLO11","authors":"Dayuan Wang ,&nbsp;Min Zhang ,&nbsp;Qibing Zhu ,&nbsp;Bhesh Bhandari ,&nbsp;Luming Rui","doi":"10.1016/j.postharvbio.2026.114190","DOIUrl":"10.1016/j.postharvbio.2026.114190","url":null,"abstract":"<div><div>Citrus fruits suffer substantial postharvest losses due to fungal infections and mechanical damage. Early decay and minor injury detection of these visually inconspicuous defects on citrus fruits remains challenging in postharvest quality control. This study proposes an innovative non-destructive detection system combining UV-fluorescence imaging and advanced YOLO11 deep learning to detect early or inconspicuous defects, including fungal infections (green mold, blue mold, and sour rot) and mechanical damage (bruising and puncture). Research revealed that when fungal infection or mechanical damage ruptures the oil glands in citrus peel, the essential oils rich in polymethoxyflavones released from defective regions emit bright yellow-green fluorescence under 365 nm UV-light illumination. Among the evaluated YOLO variants, YOLO11n demonstrated superior performance with 0.939 precision, 0.933 recall, and 0.969 [email protected], effectively localizing and classifying both healthy and defective fruits across diverse citrus varieties. Grad-CAM++ visualization confirmed that the model focused on genuine fluorescent defect regions while disregarding non-defective fluorescent features. The high-quality fluorescence imaging data create nearly ideal conditions for detection—uniform dark backgrounds with bright defect signals—enhancing detection capability and enabling deep learning models to achieve superior accuracy. This UV fluorescence-YOLO11 framework provides a low-cost, high-quality, explainable, and readily deployable solution for automated citrus quality inspection, potentially reducing significant postharvest losses in the global citrus supply chain.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"235 ","pages":"Article 114190"},"PeriodicalIF":6.8,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146015536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of novel natural cinnamic acid derivatives enabled by Ugi reaction and their potential applications","authors":"Hexiang Wang ,&nbsp;Zhijun Qiao ,&nbsp;Ting Ye ,&nbsp;Di Liu ,&nbsp;Junkun Ren ,&nbsp;Jie Deng ,&nbsp;Yanlong Wu ,&nbsp;Longzhu Bao ,&nbsp;Hongqu Wu ,&nbsp;Xiufang Cao ,&nbsp;Fang Liu ,&nbsp;Shaoyong Ke","doi":"10.1016/j.postharvbio.2026.114180","DOIUrl":"10.1016/j.postharvbio.2026.114180","url":null,"abstract":"<div><div>Plant fungal diseases pose a serious threat to agricultural production and the quality and safety of postharvest agricultural products. Therefore, developing efficient and environmentally friendly novel fungicides is of great significance. In this study, a series of novel <em>p</em>-hydroxycinnamic acid-diamide derivatives was designed and synthesized by combining naturally sourced <em>p</em>-hydroxycinnamic acid with the highly active diamide structural motif. <em>In vitro</em> antifungal activity screening revealed that several compounds exhibited significant inhibitory effects against various plant pathogenic fungi. Notably, compounds <strong>1–14</strong> and <strong>1–13</strong> showed excellent activity against <em>S. sclerotiorum</em> (EC₅₀ = 15.70 µg/mL) and <em>P. capsici</em> (EC₅₀ = 22.04 µg/mL), respectively. Compound <strong>1–13</strong> also exhibited significant <em>in vivo</em> antifungal activity against <em>P. capsici</em> on both pepper leaves and fruits, and compound <strong>1–14</strong> exhibited a clear concentration-dependent inhibitory effect on both tomato and Vitis vinifera 'Shine Muscat' against <em>B. cinerea</em>. Further investigations, including <em>in vivo</em> activity assays, propidium iodide (PI) staining, proton motive force (PMF) assessment, succinate dehydrogenase (SDH) activity tests, and scanning electron microscopy (SEM) observations, indicated that the target compounds exert their antifungal effects through multiple mechanisms. These include disrupting cell membrane integrity, interfering with energy metabolism, and inhibiting SDH enzyme activity. Molecular docking and molecular dynamics simulations revealed a stable binding mode between the compounds and the SDH active pocket. Density functional theory (DFT) calculations further verified their electronic structures and stability. This study provides valuable candidate molecules and a theoretical basis for developing novel plant-derived fungicides. The findings not only offer highly active candidate compounds for controlling postharvest diseases caused by pathogens like <em>S. sclerotiorum</em> and <em>P. capsici</em>, but the elucidated structure-activity relationships and multiple mechanisms of action also provide new insights for the rational design of pesticides based on natural products.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"235 ","pages":"Article 114180"},"PeriodicalIF":6.8,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PeCdc42 orchestrates growth, patulin secretion, and pathogenicity in Penicillium expansum through MAPK and cAMP signaling pathways","authors":"Xuemei Zhang ,&nbsp;Dandan Zhu ,&nbsp;Feng Zhang ,&nbsp;Xiao Li ,&nbsp;Dov Prusky ,&nbsp;Yuanyuan Zong ,&nbsp;Yang Bi","doi":"10.1016/j.postharvbio.2026.114189","DOIUrl":"10.1016/j.postharvbio.2026.114189","url":null,"abstract":"<div><div>As a vital part of the Rho GTPase family, Cdc42 is essential for fungal growth and its ability to cause disease, however, its function in <em>Penicillium expansum</em>, a major postharvest pathogen, remains unexplored. In this study, we characterized the biological function of PeCdc42 through gene deletion. Deletion of <em>PeCdc42</em> disrupted MAPK and cAMP signaling by downregulating core regulatory genes. <em>Pecdc42</em> deletion reduces colony growth, biomass, and spore formation, and alters the morphology of mycelium and spores. Furthermore, <em>PeCdc42</em> deletion led to reduced patulin biosynthesis. Pathogenicity assays revealed diminished virulence of the pathogen on apple and pear fruit, with reduced lesion formation and depth, lower expression of genes encoding extracellular enzymes, and lower levels of reactive oxygen species (ROS). Collectively, our findings demonstrate that PeCdc42 is a central regulator of growth, secondary metabolism, and pathogenicity in <em>P. expansum</em>, offering new perspectives on fungal disease mechanisms and possible targets for controlling postharvest diseases.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"235 ","pages":"Article 114189"},"PeriodicalIF":6.8,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146026206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gallic acid-grafted hardwood lignin: A bio-based antioxidant with enhanced biological properties","authors":"Akhmadjon Sultanov ,&nbsp;Eko Setio Wibowo ,&nbsp;Byung-Dae Park ,&nbsp;Young-Je Cho","doi":"10.1016/j.postharvbio.2026.114166","DOIUrl":"10.1016/j.postharvbio.2026.114166","url":null,"abstract":"<div><div>A bio-based antioxidant was developed by grafting gallic acid (GA) onto hardwood lignin (HWL) via multi-step esterification. Industrial HWL, rich in aromatic phenylpropanoid units but deficient in free phenolic OH groups, was structurally modified to enhance radical-scavenging capacity. The synthesis involved acetylation of GA’s phenolic OH groups, conversion to acid chloride, esterification with HWL under basic catalysis, and deprotection to restore phenolic functionalities. ATR–FTIR confirmed loss of acetyl C<img>O bands and intensified OH stretching; gel permeation chromatography indicated improved molecular homogeneity. Quantitative ³¹P NMR showed a fourfold increase in phenolic OH content (4.58–19.64 mmol/g) and near-complete conversion of aliphatic OH groups to esters. Antioxidant assays revealed 98.71 % DPPH scavenging and complete ABTS neutralization, outperforming native HWL and approaching the efficacy of butylated hydroxyanisole. GA-grafted HWL retained the lignin backbone and exhibited enhanced antioxidant properties, enabling lignin waste valorization and supporting sustainable applications for food as additives to improve antioxidant efficiency.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114166"},"PeriodicalIF":6.8,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LcMYB6 transcription factor confers resistance to pericarp dehydration and browning in litchi fruit through the regulation of aquaporin genes","authors":"Benfeng Zhang ,&nbsp;Jialiang Liu ,&nbsp;Yicheng Yang ,&nbsp;Jiabao Wang ,&nbsp;Gangshuai Liu ,&nbsp;Lisha Zhu ,&nbsp;Xiangbin Xu ,&nbsp;Zhengke Zhang","doi":"10.1016/j.postharvbio.2026.114188","DOIUrl":"10.1016/j.postharvbio.2026.114188","url":null,"abstract":"<div><div>Pericarp dehydration is a primary factor contributing to litchi fruit browning, severely limiting the marketability of the product. Aquaporins (AQPs), a class of multifunctional membrane proteins, play a pivotal role in regulating the transmembrane transport of water in plants. However, the specific involvement of AQPs in the dehydration and browning of litchi fruit, along with their underlying regulatory mechanisms, remain to be fully elucidated. The results of the present study demonstrated that control (unpackaged) litchi fruit exhibited complete browning, which was accompanied by severe weight loss (10.2 %), discoloration, and membrane damage after 60 h of storage. These rapid physiological changes were alleviated by the use of polyethylene bag packaging, an effective high-humidity maintenance method. Transcriptomic analysis revealed differential expression of 12 AQP genes across the experimental groups, among which four genes (<em>LcPIP1;1</em>, <em>LcPIP2;2</em>, <em>LcPIP2;5</em> and <em>LcSIP2;1</em>) were upregulated, while eight genes (<em>LcPIP2;7</em>, <em>LcTIP1;2</em>, <em>LcTIP2;1</em>, <em>LcTIP4;1</em>, <em>LcTIP1;1</em>, <em>LcSIP2;2</em>, <em>LcNIP1;1</em> and <em>LcNIP1;2</em>) were downregulated in control fruit during storage. The changes in the expression of most AQP genes observed in control fruit were substantially counteracted by packaging treatment, thereby ameliorating pericarp dehydration and browning in litchi fruit. Furthermore, LcMYB6, a MYB transcription factor that is induced by packaging treatment, binds to the promoters of <em>LcPIP1;1</em> and <em>LcPIP2;5</em>. Promoter binding assays verified that LcMYB6 is a nuclear protein that directly represses the transcription of <em>LcPIP1;1</em> and <em>LcPIP2;5.</em> Transient silencing of <em>LcMYB6</em> promoted the upregulation of <em>LcPIP1;1</em> and <em>LcPIP2;5</em> expression while accelerating pericarp dehydration and browning. These results indicate that LcMYB6 may increase litchi dehydration resistance by directly repressing the transcription of AQP genes (<em>LcPIP1;1</em> and <em>LcPIP2;5</em>), potentially offering new strategies for controlling pericarp browning in litchi fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114188"},"PeriodicalIF":6.8,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the combination of lactic acid, ε-poly-L-lysine and sodium isoascorbate on the swelling, microbiomics variation, and quality attributes of vacuum-packed fresh cut lotus root","authors":"Jiahui Jiang ,&nbsp;Qianlong Shi ,&nbsp;Dan Li ,&nbsp;Qingmin Chen ,&nbsp;Cong Han ,&nbsp;Jing Wei ,&nbsp;Maorun Fu ,&nbsp;Huaimei Cao ,&nbsp;Xiaofei Xin","doi":"10.1016/j.postharvbio.2026.114184","DOIUrl":"10.1016/j.postharvbio.2026.114184","url":null,"abstract":"<div><div>Vacuum-packed lotus roots slices are prone to swelling and quality deterioration when stored above 15 °C. Our study aimed to investigate the effect of combined treatment of 0.7 % lactic acid (LA), 0.7 g L⁻¹ ε-poly-<span>L</span>-lysine (ε-PL), and 0.3 % sodium isoascorbate (SI) solution on the swelling, microbial growth, antioxidant and quality characteristics of vacuum-packed lotus root slices stored at 20 °C for 6 d. Results showed that, the primary bacterial genera responsible for bag swelling were identified as <em>Tolumonas</em>, <em>Aeromonas</em>, and <em>Enterobacter,</em> utilizing 16S rDNA amplicon sequencing. Compared with the control, the LA + ε-PL + SI treatment exhibited inhibitory effect on these genera, reduced the relative abundance of <em>Tolumonas</em>, <em>Aeromonas</em>, and <em>Enterobacter</em> from 65.97 %, 13.20 %, and 3.85–1.07 %, 0.20 %, and 0.44 %, respectively, yielding suppression rates of 98.4 %, 98.5 %, and 88.6 %, thus, effectively preventing swelling. Moreover, lotus root treated with LA + ε-PL + SI showed the better color, quality attributes. These findings indicated that the LA + ε-PL + SI treatment successfully prevented swelling, inhibited microbial growth, prolonged the shelf life, and preserved the better quality of vacuum-packed fresh cut lotus root.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"234 ","pages":"Article 114184"},"PeriodicalIF":6.8,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}