Postharvest Biology and Technology最新文献

{"title":"Phlorotannins extracted from Sargassum ilicifolium as a way to control spoilage molds and extend the shelf life of Agaricus bisporus mushrooms","authors":"Parinaz Gholami ,&nbsp;Sara Chadorshabi ,&nbsp;Ehsan Shamloo ,&nbsp;Salim Sharifian ,&nbsp;Maryam Mahmoudzadeh","doi":"10.1016/j.postharvbio.2024.113267","DOIUrl":"10.1016/j.postharvbio.2024.113267","url":null,"abstract":"<div><div>Phlorotannins (PTs) are compounds with notable bioactive characteristics obtained from brown algae. In the present study, PTs from the <em>Sargassum ilicifolium</em> seaweed were extracted, characterized, and evaluated for their anti-polyphenol oxidase (PPO) enzyme activity and in vitro antifungal activity against spoilage molds (<em>Aspergillus flavus</em> and <em>Penicillium expansum</em>) of button mushrooms. Then, PTs were used as a coating in combination with alginate (AL) to improve the shelf life of <em>Agaricus bisporus</em> mushrooms under storage conditions. HPLC analysis identified phloroglucinol as the predominant component in the PT extract (18.1 %), which demonstrated an 86 ± 1.02 % radical scavenging activity in the DPPH test. The PPO enzyme activity significantly decreased from 320.34 ± 2.1 units to 34.4 ± 1.4 units in the presence of 1.5 mL of PT extract. The PT extract's antifungal properties were observed through well diffusion assays, mycelial growth inhibition, and scanning electron microscopy on treated spoilage mold colonies. Better preservation of the physicochemical properties of mushrooms coated with AL + PT was confirmed through various tests, including assessments of weight loss, browning, malondialdehyde levels, total phenol content, and vitamin C content. Additionally, sensorial tests and evaluations of visual and microstructure changes during storage at 4 °C confirmed the enhanced preservation after treatment. Findings indicated that in addition to the antifungal properties of <em>S. ilicifolium</em>-derived PT, which can act as a natural antifungal, this substance, when used in combination with AL coating, can increase the shelf life of <em>A. bisporus</em> mushrooms 12 d of storage at 4 °C.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113267"},"PeriodicalIF":6.4,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The key pathogenic factor GcSP2 of Geotrichum citri-aurantii plays an important role in disrupting citrus metabolism and immunity","authors":"Rong Liu ,&nbsp;Haoxiang Zheng ,&nbsp;Di Wang ,&nbsp;Haijue Zhang ,&nbsp;Yan Wu ,&nbsp;Xue Li ,&nbsp;Zhengfeng Liu ,&nbsp;QingJun Kong ,&nbsp;Xueyan Ren","doi":"10.1016/j.postharvbio.2024.113253","DOIUrl":"10.1016/j.postharvbio.2024.113253","url":null,"abstract":"<div><div>The pathogen <em>Geotrichum citri-aurantii</em> (<em>G. citri-aurantii</em>) causes postharvest sour rot disease in citrus fruits worldwide, severely impacting citrus economic value. However, the pathogenic mechanisms of this fungus remain inadequately understood. Here, we identified 15 candidate effector proteins from <em>G. citri-aurantii</em> genome, of which five were highly expressed during infection and were capable of suppressing BAX-induced cell death, indicating their role in inhibiting plant immunity. Gene expression analysis showed that these five effector proteins primarily induced the upregulation of pattern-triggered immunity (PTI)-related gene expression. Diaminobenzidine (DAB) staining results indicated that only <em>GcSP2</em> triggered reactive oxygen species (ROS) burst. Notably, <em>GcSP2</em> contains a known carbohydrate-binding module 1 (CBM1) domain and exhibits low overall conservation. Gene knockout experiments revealed that the absence of <em>GcSP2</em> delayed disease onset by 1–2 days and significantly reduced lesion size, establishing it as a key pathogenic factor. Assessments of total phenols, flavonoids, and pathogenesis-related protein expression indicated that <em>GcSP2</em> significantly affects citrus metabolism at 72 hours post-infection.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113253"},"PeriodicalIF":6.4,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2,4-Epibrassinolide delays lignification, softening, and quality deterioration during asparagus storage","authors":"Jiachang Xiao ,&nbsp;Junting Liu ,&nbsp;Zheng Guo ,&nbsp;Peiran Chen ,&nbsp;Fengyun Lei ,&nbsp;Wei Lu ,&nbsp;Chengyao Jiang ,&nbsp;Yanwen Li ,&nbsp;Mengyao Li ,&nbsp;Yangxia Zheng","doi":"10.1016/j.postharvbio.2024.113270","DOIUrl":"10.1016/j.postharvbio.2024.113270","url":null,"abstract":"<div><div>Asparagus is prone to weight loss and aging after harvest. 2,4-Epibrassinolide (EBR), a brassinosteroid hormone, is widely involved in plant growth, development, and stress response. This study investigated the effects of different EBR treatments (0, 2.5, 5, 7.5, 10, and 15 μmol L⁻¹) on the sensory quality and biochemical substance content of asparagus stored at 4°C for 5, 10, 15, 20, and 25 d. The results showed that EBR application effectively mitigates weight loss and quality deterioration during storage. This effect was evidenced by higher firmness, total chlorophyll content, soluble protein, total saponins, mechanical tissue thickness, and lower lignin content and malondialdehyde levels. Moreover, EBR enhances the antioxidant capacity of asparagus by regulating the enzymatic antioxidant system (superoxide dismutase, catalase, peroxidase, and polyphenol oxidase activities) and the non-enzymatic antioxidant system (DPPH free radical scavenging ability, vitamin C, total phenol, total flavonoids, rutin, amino acids, and proline content), thus, reducing oxidative damage. Furthermore, transcriptome analysis revealed that the softening and aging of asparagus are closely related to phenylpropanoid biosynthesis and starch and sucrose metabolism. 2,4-Epibrassinolide inhibits lignification by reducing the expression of lignin synthesis-related genes (<em>PAL</em>, <em>C4H</em>, <em>4CL</em>, <em>COMT</em>, <em>CCR</em>, <em>CAD</em>, and <em>POD</em>). Additionally, EBR delays asparagus softening by suppressing cell wall degradation-related genes (<em>Egase</em>, <em>β-BGL</em>, <em>PE</em>, and <em>PG</em>). Weighted Gene Co-expression Network Analysis (WGCNA) identified 12 lignification-related candidate genes and five softening-related candidate genes. Additionally, the RT-qPCR expression of the 17 candidate genes was consistent with the transcriptome results. Altogether, this study provides a basis for EBR application in asparagus storage and preservation and offers insights into the molecular mechanisms by which EBR delays lignification and softening in asparagus.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113270"},"PeriodicalIF":6.4,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wound induces sequential synthesis and conductance of different phytohormones in apple wounds","authors":"Huiwen Deng ,&nbsp;Di Gong ,&nbsp;Li Ma ,&nbsp;Pengdong Xie ,&nbsp;Dov Prusky ,&nbsp;Yang Bi","doi":"10.1016/j.postharvbio.2024.113261","DOIUrl":"10.1016/j.postharvbio.2024.113261","url":null,"abstract":"<div><div>Phytohormones synthesis involved in wound stress in fruit and vegetables has been reported, whether wound induces the phytohormones synthesis and conduction in fruit, and the responses the time sequence has not been reported. In this study, the apples were used as the experimental material, transcriptomic profiling indicated that differentially expressed genes (DEGs) mainly enriched in salicylic acid (SA), ethylene (ET), abscisic acid (ABA) and jasmonic acid (JA) synthesis and signaling at wounds of apple fruit. Wound significantly up-regulates the expression of the above phytohormone synthesis-related genes, and promotes the biosynthesis of SA, ABA, ET and JA at fruit wounds. There are temporal differences in the production of these hormones. SA was synthesized earliest, followed by ABA and ET, and finally JA. Wound also up-regulated the expression of the above-mentioned gene related to hormone transduction and promoted the signal transduction of SA, ABA, ET and JA. In conclusion, wound can induce the sequential synthesis and conductance of different phytohormones in apple wounds. This study reveals the sequential synthesis and signaling of key phytohormones in apple fruit in response to wounding, providing valuable insights for improving postharvest quality and stress resistance strategies.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113261"},"PeriodicalIF":6.4,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histone deacetylase MdHDT3 suppresses ethylene biosynthesis by deacetylating MdACS1 and MdACO1 during apple fruit ripening","authors":"Yuqing Wang ,&nbsp;Jiamao Sun ,&nbsp;Yajing Wei ,&nbsp;Shipeng Wei ,&nbsp;Yanan Wang ,&nbsp;Aide Wang ,&nbsp;Dongmei Tan ,&nbsp;Yinglin Ji","doi":"10.1016/j.postharvbio.2024.113269","DOIUrl":"10.1016/j.postharvbio.2024.113269","url":null,"abstract":"<div><div>Apple (<em>Malus domestica</em>) is a climacteric fruit whose ripening is primarily controlled by ethylene. Histone acetylation functions in ethylene biosynthesis during apple ripening, but its underlying molecular mechanisms in ethylene biosynthesis are unclear. Therefore, this study aims to investigate the effects and potential molecular mechanisms of histone modifications in ethylene synthesis during apple fruit ripening. RNA sequencing was performed on apple fruit treated with histone deacetylase inhibitor sodium butyrate to identify histone deacetylases. Additionally, we examined the effects of a specific histone deacetylase on ethylene production and ethylene-related genes. Among the six histone deacetylases identified in the apple fruit, <em>MdHDT3</em> had the highest expression level during ripening. Treatment with sodium butyrate significantly inhibited <em>MdHDT3</em> expression and promoted ethylene biosynthesis in apple fruit. Additionally, MdHDT3 suppressed the expression of key genes involved in ethylene biosynthesis (<em>MdACS1</em> and <em>MdACO1</em>) via deacetylation, thereby negatively regulating ethylene biosynthesis. Conclusively, this study provides insight into the molecular mechanism by which the histone deacetylase MdHDT3 inhibits ethylene synthesis in apple fruit from an epigenetic perspective.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113269"},"PeriodicalIF":6.4,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen sulfide enhances PpbHLH3-controlled sucrose accumulation in peach chilling tolerance","authors":"Li Wang ,&nbsp;Yanyan Wang ,&nbsp;Qingyuan Song ,&nbsp;Tingyu Wu ,&nbsp;Kaili Shi ,&nbsp;Tian Qiu ,&nbsp;Jingjing Jiang ,&nbsp;Xingyue Wang ,&nbsp;Zhikang Liu ,&nbsp;Peng Jin ,&nbsp;Yonghua Zheng ,&nbsp;Dan Chen","doi":"10.1016/j.postharvbio.2024.113259","DOIUrl":"10.1016/j.postharvbio.2024.113259","url":null,"abstract":"<div><div>Chilling injury (CI) is a serious problem that restricts postharvest preservation of peach industry. Hydrogen sulfide (H₂S) treatment efficiently mitigated CI of peach, whereas the molecular mechanism of which remains elusive. In this study, results showed that H₂S treatment maintained lower internal browning index, electrolyte leakage and malondialdehyde content in comparison with control, while hypotaurine (HT, H₂S scavenger) existed with the reverse results during cold storage. Meanwhile, H₂S treatment induced higher activities and genes expression levels of sucrose synthase-synthesis (SS-s) and sucrose phosphate synthase (SPS), while suppressing lower these levels of vacuolar invertase (VIN) and neutral invertase (NI) compared to control and HT, which was concomitant with sucrose accumulation and decrease of fructose and glucose. Moreover, transcription factor PpbHLH3 was shown to respond to low temperature and H₂S treatment and located in nucleus. Results of PpbHLH3 overexpression showed that <em>PpSPS</em> and <em>PpSS-s</em> expressions were elevated, while <em>PpVIN</em> and <em>PpNI</em> expressions were weakened in peach fruit. Further investigation revealed that PpbHLH3 could activate and inhibit the expression of <em>PpSPS</em> and <em>PpVIN</em> by binding <em>PpSPS</em> and <em>PpVIN</em> promoter E-box, respectively. Consequently, these findings revealed that H₂S treatment promoted PpbHLH3-mediated sucrose accumulation by improving the gene expression and activities of SS and SPS, and inhibiting the gene expression and activities of VIN and NI, contributing to the enhancement of cold resistance in cold-stored peach fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113259"},"PeriodicalIF":6.4,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethylene mitigates nut decay and improves nut quality of Torreya grandis during postharvest by changing microbial community composition","authors":"Jinwei Suo ,&nbsp;Zhanhua Zhou ,&nbsp;Mohamed A. Farag ,&nbsp;Zuying Zhang ,&nbsp;Jiasheng Wu ,&nbsp;Yuanyuan Hu ,&nbsp;Lili Song","doi":"10.1016/j.postharvbio.2024.113250","DOIUrl":"10.1016/j.postharvbio.2024.113250","url":null,"abstract":"<div><div><em>Torreya grandis</em> nuts require postharvest ripening for oil accumulation, with ethephon (ETH) commonly used to induce such process, thereby impacting the endophytic microbial community. In this study, shelled <em>T</em>. <em>grandis</em> nuts were divided into two groups: one treated ETH and the other with water (CK). The ETH treatment expedited nutrient metabolism from day 5 till day 10 as manifested by a decline in starch content, while increasing oil and soluble protein, enhancing enzyme activity, and reducing nut decay incidence. ETH-treated nut exhibited greater bacterial and fungal diversity compared with CK, along with more distinct microbial biomarkers. Structural equation modeling linked with ETH treatment revealed that increased oil content was mediated via increased abundance of <em>Myxozyma</em>, <em>Rhodobacter</em>, and pyruvate kinase activity. <em>Chalara</em>, a fungal biomarker, was strongly correlated with decay incidence. These findings offer valuable biomarkers for mitigating decay and improving <em>T. grandis</em> nuts quality during post-ripening stage in the future.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113250"},"PeriodicalIF":6.4,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive metabolome and transcriptome analyses shed light on the regulation of SlNF-YA3b in carotenoid biosynthesis in tomato fruit","authors":"Peiwen Wang ,&nbsp;Siyu Ran ,&nbsp;Yuanhang Xu ,&nbsp;Fulei Mo ,&nbsp;Fengshuo Li ,&nbsp;Rui Lv ,&nbsp;Fanyue Meng ,&nbsp;Huixin Zhang ,&nbsp;Yuxin Zou ,&nbsp;Lei Yu ,&nbsp;Tianyue Yu ,&nbsp;Mozhen Cheng ,&nbsp;Yang Liu ,&nbsp;Xiuling Chen ,&nbsp;Xiaoxuan Zhang ,&nbsp;Aoxue Wang","doi":"10.1016/j.postharvbio.2024.113263","DOIUrl":"10.1016/j.postharvbio.2024.113263","url":null,"abstract":"<div><div>Fruit are an important source of human dietary nutrition, in which carotenoids are crucial for their appearance and nutritional quality. However, the regulatory network of carotenoid biosynthesis in the fruit of horticultural crops remains obscure. Here, a natural tomato mutant, <em>yellowing mutant</em> (<em>ym</em>), in the genetic background of <em>Solanum lycopersicum</em> cultivar ‘Zhongshu 4’ (ZS4) was investigated. Phenotypic analysis showed that the coloring of <em>ym</em> fruit was delayed compared to their wild type, and carotenoid content in <em>ym</em> fruit was significantly lower than that of ZS4 fruit. Integrative metabolome and transcriptome profiling was used to analyze the dynamic changes of carotenoid metabolite content and gene expression in ZS4 and <em>ym</em> fruit during ripening, and differences in carotenoid metabolite content and gene expression between ZS4 and <em>ym</em> fruit were compared. In contrast to ZS4 fruit, the content of carotenes dramatically decreased in <em>ym</em> fruit, of which phytoene and lycopene levels were down-regulated in <em>ym</em> throughout fruit ripening. In the process of fruit ripening, the transcriptome fluctuation of <em>ym</em> was obviously stronger than that of ZS4. Differences in gene expression between ZS4 and <em>ym</em> gradually reduced with fruit ripening. Furthermore, 105 consistently up-regulated and 113 consistently down-regulated genes were found in <em>ym</em> fruit during ripening. KEGG pathway enrichment analyses indicated that differentially expressed genes between ZS4 and <em>ym</em> were implicated in the carotenoid biosynthesis pathway. Correlation analysis showed that 28 genes were positively correlated with phytoene and lycopene content, including <em>SlNF-YA3b</em> (Solyc12G000315) encoding an NF-YA subunit of nuclear factor Y (NF-Y) transcription factors. Expression analysis exhibited that <em>SlNF-YA3b</em> presented a ripening-related expression pattern. Virus-induced gene silencing demonstrated that <em>SlNF-YA3b</em> positively regulated carotenoid accumulation in tomato fruit<em>.</em> Yeast-one hybrid and transcriptional activity assays showed that SlNF-YA3b could bind to the promoter of the carotenogenic gene <em>SlPDS</em> (Solyc03G003570) and promote its transcription. These data suggest that SlNF-YA3b may participate in the regulation of carotenoid biosynthesis in tomato fruit by directly activating the expression of <em>SlPDS</em>. Our findings not only achieve deeper insights into the regulatory mechanisms of carotenoid biosynthesis in the fruit of horticultural crops but also provide better guidance for the improvement of fruit quality maintenance technologies.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113263"},"PeriodicalIF":6.4,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MaERF9 and MaERF113 transcription factors involve in chilling injury development by regulating membrane lipid metabolism of postharvest banana fruit","authors":"Lijuan Bai ,&nbsp;Yanting Wu ,&nbsp;Hetong Lin ,&nbsp;Wenbing Su ,&nbsp;Zhongqi Fan","doi":"10.1016/j.postharvbio.2024.113230","DOIUrl":"10.1016/j.postharvbio.2024.113230","url":null,"abstract":"<div><div>Banana is a cold-sensitive fruit, which exhibits vulnerability to chilling injury (CI), causing browning, pitting, even abnormal ripening, resulting in quality deterioration and economic loss. CI development of banana fruit was caused by membrane integrity damage and was related to the enzymatic and genetic manipulation of membrane lipid metabolism pathway. The involvement of Ethylene Response Factor (ERF) transcription factors (TFs) in membrane lipid-mediated CI development of cold-sensitive fruit, such as banana, remains largely unknown. In this study, we found that cold treatment increased CI index, cell membrane permeability and MDA content, decreased hue angle (<em>h°</em>) value. The expressions of membrane lipids metabolism genes, including <em>MaDGK, MaPLA, MaLipase and MaLOX,</em> were upregulated by cold treatment. Also, two banana ERF TFs, designated as <em>MaERF9</em> and <em>MaERF113</em>, were induced by cold environment and displayed transactivation activities. Additionally, the electrophoretic mobility shift assay (EMSA) and transient expression analysis showed that MaERF9 and MaERF113 directly bound to the GCC-box elements in the promoters of <em>MaDGK, MaPLA, MaLipase and MaLOX</em>, and activated their expressions. These findings imply that MaERF9 and MaERF113 involved in CI development of banana fruit, via affecting membrane lipid metabolism. Collectively, our results provide new insights into the transcriptional regulation network regulating CI development of banana fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113230"},"PeriodicalIF":6.4,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptomics of Monilinia fructicola - resistant and - susceptible peach fruit reveals gene networks associated with peach resistance to brown rot disease","authors":"Shenge Li,&nbsp;Jianlan Xu,&nbsp;ZhiXiang Cai,&nbsp;Ruijuan Ma,&nbsp;Mingliang Yu,&nbsp;Zhijun Shen","doi":"10.1016/j.postharvbio.2024.113254","DOIUrl":"10.1016/j.postharvbio.2024.113254","url":null,"abstract":"<div><div>Peach brown rot, primarily caused by <em>Monilinia fructicola</em>, is a major cause of fruit loss during the post-harvest period. Although some varieties with improved brown rot tolerance have been identified, the genetic basis of brown rot resistance remains unclear. In this study, a comparison of the transcriptome response to <em>M. fructicola</em> infection in peach the resistant ‘Xiahui 8’ (XH8) and susceptible variety ‘Xiahui 6’ (XH6) indicating 622 resistance-associated differentially expressed genes (DEGs) that could be consistently detected throughout the 12–48 h postinfection period. Gene Ontology (GO) enrichment analysis of the 292 upregulated resistance associated DEGs indicated an enrichment (54/292, <em>p</em>＜0.05) in defense and stimulus response terms. Co-expression network analysis indicated nine gene modules of co-expressed genes, with two modules linked to <em>PpNLR</em> genes and showing associations with DEGs related to calcium signaling, autophagy, lignin biosynthesis, and pathogenesis-related proteins (PRs). Interestingly, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis suggested enrichment in stress-related pathways such as plant-pathogen interaction, flavonoid biosynthesis, phenylpropanoid biosynthesis and amino sugar and nucleotide sugar metabolism. Moreover, relative to XH6, <em>M. fructicola</em> infection of XH8 resulted in increases in the expression of <em>PpNLRs</em> and <em>PpCNGCs</em>, the induction of cell death, the activities of chitinases and <em>β</em>-1,3-glucanes, and the peach contents of calcium, reactive oxygen species, lignin and anthocyanins. These findings provide a greater insight into the genetic basis of peach fruit resistance to brown rot disease and can ultimately aid in breeding resistant peach varieties.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113254"},"PeriodicalIF":6.4,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}