Pub Date : 2025-02-16DOI: 10.1016/j.postharvbio.2025.113458
Ranran Xu , Wanqing Liu , Jiahua Zhou , Shuaiqi Zhang , Lihua Jiang , Bangdi Liu , Baogang Wang
Postharvest diseases caused by fungal infections in nectarine fruit seriously affect its commodity value and economic benefits. The effects and mechanisms of application of amino acid derivative ectoine against decay mold disease on postharvest nectarine fruit were evaluated. Ectoine remarkably reduced the occurrence of brown rot in nectarines after inoculation with Monilinia fructicola, which was most likely achieved by enhancing the resistance of the fruit. The expression of genes such as phenylalanine ammonia-lyase in ethylene transduction pathway were downregulated and the expressions of jasmonic acid and salicylic acid were upregulated with ectoine treatment. Moreover, the activities of key enzymes such as β-1,3-glucanase and superoxide dismutase for antibacterial and antioxidation activities, respectively, were enhanced. Phenylpropane biosynthesis was then activated and antioxidant capacity was enhanced. As a result, ectoine improved the resistance of nectarine fruit against fungal diseases. This study provides a new and promising approach to reduce postharvest decay of nectarine.
{"title":"Ectoine inhibits postharvest decay of nectarine fruit by strengthening fruit resistance","authors":"Ranran Xu , Wanqing Liu , Jiahua Zhou , Shuaiqi Zhang , Lihua Jiang , Bangdi Liu , Baogang Wang","doi":"10.1016/j.postharvbio.2025.113458","DOIUrl":"10.1016/j.postharvbio.2025.113458","url":null,"abstract":"<div><div>Postharvest diseases caused by fungal infections in nectarine fruit seriously affect its commodity value and economic benefits. The effects and mechanisms of application of amino acid derivative ectoine against decay mold disease on postharvest nectarine fruit were evaluated. Ectoine remarkably reduced the occurrence of brown rot in nectarines after inoculation with <em>Monilinia fructicola</em>, which was most likely achieved by enhancing the resistance of the fruit. The expression of genes such as phenylalanine ammonia-lyase in ethylene transduction pathway were downregulated and the expressions of jasmonic acid and salicylic acid were upregulated with ectoine treatment. Moreover, the activities of key enzymes such as β-1,3-glucanase and superoxide dismutase for antibacterial and antioxidation activities, respectively, were enhanced. Phenylpropane biosynthesis was then activated and antioxidant capacity was enhanced. As a result, ectoine improved the resistance of nectarine fruit against fungal diseases. This study provides a new and promising approach to reduce postharvest decay of nectarine.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113458"},"PeriodicalIF":6.4,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-16DOI: 10.1016/j.postharvbio.2025.113457
Chenchen Wu , Chuanlong Men , Yaqian Wang , Tingting Fan , Changhong Liu , Lei Zheng
Postharvest strawberry is susceptible to mechanical damage and fungal attack, which poses serious storage challenges. In this study, senescence of strawberry fruit was successfully delayed using 0.25, 0.5 and 1 g L−1 α-lipoic acid (α-LA), and microbial proliferation was effectively inhibited during the storage. In particular, the treatment at with 0.5 g L−1 α-LA was the most effective, significantly reducing the decay rate and weight loss, while sustaining the firmness, TSS content and color. Strawberry fruit treated with α-LA showed higher total phenolics, total flavonoids, total anthocyanin, ascorbic acid, and individual phenolic compounds content compared with the control. α-LA attenuated strawberry membrane lipid peroxidation by inhibiting MDA and H2O2 production, and increasing antioxidant capacity. Transcriptomic analysis revealed significant enrichment of genes related to phenylpropanoid biosynthesis, and the transcription factors FaMYC2 and FaMYB308 might act as negative regulators in phenolic metabolism. In addition, α-LA remarkably enhanced the expression of key genes related to phenolic metabolism and anthocyanin synthesis. In summary, α-LA maintained quality by modulating phenolic metabolism and antioxidant capacity in postharvest strawberry.
{"title":"α-lipoic acid maintains quality by modulating phenolic metabolism and antioxidant capacity in postharvest strawberry","authors":"Chenchen Wu , Chuanlong Men , Yaqian Wang , Tingting Fan , Changhong Liu , Lei Zheng","doi":"10.1016/j.postharvbio.2025.113457","DOIUrl":"10.1016/j.postharvbio.2025.113457","url":null,"abstract":"<div><div>Postharvest strawberry is susceptible to mechanical damage and fungal attack, which poses serious storage challenges. In this study, senescence of strawberry fruit was successfully delayed using 0.25, 0.5 and 1 g L<sup>−1</sup> α-lipoic acid (α-LA), and microbial proliferation was effectively inhibited during the storage. In particular, the treatment at with 0.5 g L<sup>−1</sup> α-LA was the most effective, significantly reducing the decay rate and weight loss, while sustaining the firmness, TSS content and color. Strawberry fruit treated with α-LA showed higher total phenolics, total flavonoids, total anthocyanin, ascorbic acid, and individual phenolic compounds content compared with the control. α-LA attenuated strawberry membrane lipid peroxidation by inhibiting MDA and H<sub>2</sub>O<sub>2</sub> production, and increasing antioxidant capacity. Transcriptomic analysis revealed significant enrichment of genes related to phenylpropanoid biosynthesis, and the transcription factors FaMYC2 and FaMYB308 might act as negative regulators in phenolic metabolism. In addition, α-LA remarkably enhanced the expression of key genes related to phenolic metabolism and anthocyanin synthesis. In summary, α-LA maintained quality by modulating phenolic metabolism and antioxidant capacity in postharvest strawberry.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113457"},"PeriodicalIF":6.4,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-15DOI: 10.1016/j.postharvbio.2025.113445
Yunyun Weng , Sitong Wang , Tongfei Niu , Yuying Li , Chengwei Song , Qi Guo , Linfeng Chen , Shaodan Liu , Xiaogai Hou , Lili Guo
Vase-inserted cut tree peony flowers have a short viewing period and research focusing on prolonging the flowering duration is limited. This study investigated the effects of spermidine (Spd) on tree peony cut flowers inserted in vases containing different concentrations of Spd solutions. Paeonia suffruticosa ‘Luoyang Hong’ cut flowers at the full blooming stage were used as the test material, and floral aroma, phenotypic characteristics, and physiological indices were selected as the evaluation indicators. The results indicated that a concentration of 400 μmol·L−1 Spd significantly increased the contents of terpenoids, ketones, and alcohols, while 5 μmol·L−1 Spd significantly elevated the levels of hydrocarbons and benzene. The aldehyde content in the vase-inserted tree peony cut flowers was significantly enhanced after the addition of 300 μmol·L−1 Spd. Moreover, 5 μmol·L−1 Spd significantly improved the fresh weight, flower diameter, and flower height, prolonged the flowering period, increased the superoxide dismutase, peroxidase activities, and soluble protein content, and decreased the activities of ACC oxidase, ACC synthetase, and malondialdehyde (MDA). In conclusion, the addition of 5 μmol·L−1 Spd effectively enhanced the contents of floral phenylcyclic compounds, maintained the ornamental quality, and delayed the senescence of vase-inserted cut tree peony flowers.
{"title":"Effect of spermidine on the quality of vase-inserted Paeonia suffruticosa ‘Luoyang Hong’","authors":"Yunyun Weng , Sitong Wang , Tongfei Niu , Yuying Li , Chengwei Song , Qi Guo , Linfeng Chen , Shaodan Liu , Xiaogai Hou , Lili Guo","doi":"10.1016/j.postharvbio.2025.113445","DOIUrl":"10.1016/j.postharvbio.2025.113445","url":null,"abstract":"<div><div>Vase-inserted cut tree peony flowers have a short viewing period and research focusing on prolonging the flowering duration is limited. This study investigated the effects of spermidine (Spd) on tree peony cut flowers inserted in vases containing different concentrations of Spd solutions. <em>Paeonia suffruticosa</em> ‘Luoyang Hong’ cut flowers at the full blooming stage were used as the test material, and floral aroma, phenotypic characteristics, and physiological indices were selected as the evaluation indicators. The results indicated that a concentration of 400 μmol·L<sup>−1</sup> Spd significantly increased the contents of terpenoids, ketones, and alcohols, while 5 μmol·L<sup>−1</sup> Spd significantly elevated the levels of hydrocarbons and benzene. The aldehyde content in the vase-inserted tree peony cut flowers was significantly enhanced after the addition of 300 μmol·L<sup>−1</sup> Spd. Moreover, 5 μmol·L<sup>−1</sup> Spd significantly improved the fresh weight, flower diameter, and flower height, prolonged the flowering period, increased the superoxide dismutase, peroxidase activities, and soluble protein content, and decreased the activities of ACC oxidase, ACC synthetase, and malondialdehyde (MDA). In conclusion, the addition of 5 μmol·L<sup>−1</sup> Spd effectively enhanced the contents of floral phenylcyclic compounds, maintained the ornamental quality, and delayed the senescence of vase-inserted cut tree peony flowers.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113445"},"PeriodicalIF":6.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-15DOI: 10.1016/j.postharvbio.2025.113451
Fan Liu , Xueli Sun , Jia He , Jingjing Shan , Ou Sheng , Tongxin Dou , Yaoyao Li , Weidi He , Qiaosong Yang , Chunhua Hu , Huijun Gao , Xinxiang Peng , Ganjun Yi , Fangcheng Bi
Fruit ripening is a complex process involving physiological and metabolic changes that influence fruit color, flavor, aroma, and texture, which is modulated by various hormones and key genes. NAC with transmembrane motif-like (NTL) transcription factors belong to the NAC gene family with transmembrane motifs at their C-terminus and play important roles in plant growth, development, and stress responses. However, the role of NTL during fruit ripening remains enigmatic. Here, we identified a NTL gene, MaNAC169, that was regulated by ethylene during banana fruit ripening. MaNAC169 contained a conserved TM in its far C-terminal region and localized in the nucleus and plasma membrane. Its active form, MaNAC169-ΔC4, acted as a transcriptional activator. Importantly, MaNAC169 directly bound to the promoters of MaACO1, MaEXP2, MaXET1, and MaGWD1 and positively regulated their gene expression. MaNAC169 also activated its own expression and could form a dimer with itself. Moreover, transient MaNAC169-ΔC4 overexpression in banana fruit promoted ripening by inducing target gene expression. Taken together, MaNAC169 positively modulated fruit ripening by activating the expression of ripening-associated genes. Our findings provide new insights into the transcriptional regulatory mechanisms involved in fruit ripening.
{"title":"Membrane-associated NAC transcription factor MaNAC169 is a positive regulator during banana fruit ripening","authors":"Fan Liu , Xueli Sun , Jia He , Jingjing Shan , Ou Sheng , Tongxin Dou , Yaoyao Li , Weidi He , Qiaosong Yang , Chunhua Hu , Huijun Gao , Xinxiang Peng , Ganjun Yi , Fangcheng Bi","doi":"10.1016/j.postharvbio.2025.113451","DOIUrl":"10.1016/j.postharvbio.2025.113451","url":null,"abstract":"<div><div>Fruit ripening is a complex process involving physiological and metabolic changes that influence fruit color, flavor, aroma, and texture, which is modulated by various hormones and key genes. NAC with transmembrane motif-like (NTL) transcription factors belong to the NAC gene family with transmembrane motifs at their C-terminus and play important roles in plant growth, development, and stress responses. However, the role of NTL during fruit ripening remains enigmatic. Here, we identified a NTL gene, MaNAC169, that was regulated by ethylene during banana fruit ripening. MaNAC169 contained a conserved TM in its far C-terminal region and localized in the nucleus and plasma membrane. Its active form, MaNAC169-ΔC4, acted as a transcriptional activator. Importantly, MaNAC169 directly bound to the promoters of <em>MaACO1</em>, <em>MaEXP2</em>, <em>MaXET1</em>, and <em>MaGWD1</em> and positively regulated their gene expression. MaNAC169 also activated its own expression and could form a dimer with itself. Moreover, transient <em>MaNAC169-ΔC4</em> overexpression in banana fruit promoted ripening by inducing target gene expression. Taken together, MaNAC169 positively modulated fruit ripening by activating the expression of ripening-associated genes. Our findings provide new insights into the transcriptional regulatory mechanisms involved in fruit ripening.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113451"},"PeriodicalIF":6.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-13DOI: 10.1016/j.postharvbio.2025.113454
Qingbiao Xie , Limei Huang , Qianyi Xiao , Hongli Luo , Qiannan Wang , Bang An
Postharvest diseases, particularly anthracnose caused by Colletotrichum gloeosporioides, pose significant challenges to global fruit markets by reducing quality and marketability. Although traditional fungicides like Iprodione are commonly used for disease control, their effectiveness is increasingly limited by environmental concerns and the emergence of resistant fungal strains. This study investigated the potential of berberine, a natural alkaloid, to enhance the antifungal efficacy of Iprodione. In vitro assays showed that berberine significantly improved Iprodione’s inhibitory effects on fungal growth and conidia germ tube elongation. In vivo tests on apple fruit further validated these findings, showing significant reductions in lesion when berberine and Iprodione were applied together compared to either treatment alone. Western blot analysis revealed that the combined treatment significantly decreased protein phosphorylation in C. gloeosporioides, indicating enhanced dephosphorylation effects. Transcriptomic analysis revealed that berberine downregulates key detoxification and fungicide-resistance genes, such as cytochrome P450s, amidohydrolases, DyP-type peroxidase and membrane transporters, disrupting detoxification pathways and increasing Iprodione’s toxicity. Gene knockout studies indicated that ΔCgADH1 and ΔCgDyPOD1 mutants exhibited increased sensitivity to Iprodione, highlighting their roles in resistance mechanisms. These findings suggest that berberine can synergistically enhance Iprodione’s antifungal activity, providing a promising, eco-friendly strategy for controlling postharvest anthracnose while potentially reducing fungicide usage in the fruit industry.
{"title":"Berberine Synergistically Enhances Iprodione’s Antifungal Activity Against Colletotrichum gloeosporioides","authors":"Qingbiao Xie , Limei Huang , Qianyi Xiao , Hongli Luo , Qiannan Wang , Bang An","doi":"10.1016/j.postharvbio.2025.113454","DOIUrl":"10.1016/j.postharvbio.2025.113454","url":null,"abstract":"<div><div>Postharvest diseases, particularly anthracnose caused by <em>Colletotrichum gloeosporioides</em>, pose significant challenges to global fruit markets by reducing quality and marketability. Although traditional fungicides like Iprodione are commonly used for disease control, their effectiveness is increasingly limited by environmental concerns and the emergence of resistant fungal strains. This study investigated the potential of berberine, a natural alkaloid, to enhance the antifungal efficacy of Iprodione. <em>In vitro</em> assays showed that berberine significantly improved Iprodione’s inhibitory effects on fungal growth and conidia germ tube elongation. <em>In vivo</em> tests on apple fruit further validated these findings, showing significant reductions in lesion when berberine and Iprodione were applied together compared to either treatment alone. Western blot analysis revealed that the combined treatment significantly decreased protein phosphorylation in <em>C. gloeosporioides</em>, indicating enhanced dephosphorylation effects. Transcriptomic analysis revealed that berberine downregulates key detoxification and fungicide-resistance genes, such as cytochrome P450s, amidohydrolases, DyP-type peroxidase and membrane transporters, disrupting detoxification pathways and increasing Iprodione’s toxicity. Gene knockout studies indicated that Δ<em>CgADH1</em> and Δ<em>CgDyPOD1</em> mutants exhibited increased sensitivity to Iprodione, highlighting their roles in resistance mechanisms. These findings suggest that berberine can synergistically enhance Iprodione’s antifungal activity, providing a promising, eco-friendly strategy for controlling postharvest anthracnose while potentially reducing fungicide usage in the fruit industry.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113454"},"PeriodicalIF":6.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.postharvbio.2025.113418
Yajun Wang , Shuang Min , Tian Gao , Canying Li , Yonghong Ge
This study was performed to explore the impact of atomized fumigation with basil essential oil (BEO) on the metabolism of reactive oxygen species, respiration, and energy, as well as quality parameters in blueberry fruit. Results demonstrated that BEO had minimal impact on surface color, significantly inhibited respiratory rate and ethylene release, while maintaining ascorbic acid, reducing sugar, soluble sugar, and reduced glutathione levels in blueberries. Moreover, BEO attenuated weight loss increase and inhibited hydrogen peroxide and malondialdehyde accumulation. BEO resulted in enhanced activities and gene expressions of ascorbate peroxidase, NADPH hydrogenase, superoxide dismutase, peroxidase, glutathione reductase, cytochrome C oxidase, hexokinase, glucose-phosphate isomerase, succinate dehydrogenase, and nicotinamide adenine dinucleotide kinase. Meanwhile, BEO up-regulated VcH+-ATPase and VcCa2+-ATPase expressions, as well as enhanced catalase, glucose-6-phosphate dehydrogenase, and polyphenol oxidase activities. Additionally, BEO reduced NAD/NADH levels, while increasing NADP/NADPH levels. In summary, BEO fumigation can enhance the antioxidant capacity, mitigate membrane peroxidation, modulate energy and respiration metabolism, thereby preserving the storage quality of blueberries.
{"title":"Atomized fumigation with basil essential oil regulated reactive oxygen metabolism and respiratory metabolism to maintain storage quality of blueberry fruit","authors":"Yajun Wang , Shuang Min , Tian Gao , Canying Li , Yonghong Ge","doi":"10.1016/j.postharvbio.2025.113418","DOIUrl":"10.1016/j.postharvbio.2025.113418","url":null,"abstract":"<div><div>This study was performed to explore the impact of atomized fumigation with basil essential oil (BEO) on the metabolism of reactive oxygen species, respiration, and energy, as well as quality parameters in blueberry fruit. Results demonstrated that BEO had minimal impact on surface color, significantly inhibited respiratory rate and ethylene release, while maintaining ascorbic acid, reducing sugar, soluble sugar, and reduced glutathione levels in blueberries. Moreover, BEO attenuated weight loss increase and inhibited hydrogen peroxide and malondialdehyde accumulation. BEO resulted in enhanced activities and gene expressions of ascorbate peroxidase, NADPH hydrogenase, superoxide dismutase, peroxidase, glutathione reductase, cytochrome C oxidase, hexokinase, glucose-phosphate isomerase, succinate dehydrogenase, and nicotinamide adenine dinucleotide kinase. Meanwhile, BEO up-regulated <em>VcH</em><sup><em>+</em></sup><em>-ATPase</em> and <em>VcCa</em><sup><em>2+</em></sup><em>-ATPase</em> expressions, as well as enhanced catalase, glucose-6-phosphate dehydrogenase, and polyphenol oxidase activities. Additionally, BEO reduced NAD/NADH levels, while increasing NADP/NADPH levels. In summary, BEO fumigation can enhance the antioxidant capacity, mitigate membrane peroxidation, modulate energy and respiration metabolism, thereby preserving the storage quality of blueberries.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113418"},"PeriodicalIF":6.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11DOI: 10.1016/j.postharvbio.2025.113433
Huiwen Wang , Tonghao Cui , Jiaqi Wang , Ruohan Ou , Yilong Liu , Bo Zhang , Changjie Xu , Changqing Zhu , Miaojin Chen , Yanna Shi , Yuanjiang Pan , Kunsong Chen , Xiaoyong Zhao , Xian Li
Post-harvest chilling injury (CI) is a severe problem that causes great economic losses. The peach is an important fruit consumed worldwide but susceptible to CI under post-harvest storage, and thus serves as the typical model for CI research. Protein N-glycosylation plays a vital role under abiotic stress in plants. Here, a CI-associated up-regulation of N-glycosylation was observed in peach fruit stored at 5 ℃. An integrative N-glycomics and N-glycoproteomics analysis by reactive matrix-assisted laser desorption ionization time-of-flight mass spectrometry and 18O-labeled tagging with nano-liquid chromatography-electrospray ionization tandem mass spectrometry, respectively, was developed to provide first insights into N-glycosylation remodeling for fruit tolerance to CI. On one side, 27 N-glycans were identified by N-glycomics analysis and seven complex-type N-glycans were substantially up-regulated in CI group. On the other side, N-glycoproteomics analysis identified 1002 N-glycosites on 732 N-glycoproteins. Differential and gene set analyses revealed 88 common N-glycoproteins, based on which three KEGG pathways including metabolic pathways, amino sugar & nucleotide sugar metabolism, and protein processing in endoplasmic reticulum (ER) were highly enriched. Among N-glycoproteins in top three enriched KEGG pathways, 29 N-glycosites were anchored and nearly 80 % of them were up-regulated in CI group. The corresponding 22 hub N-glycoproteins were primarily involved in CI-related biological processes such as phytohormone signaling, redox homeostasis, biotic stress, ER quality control, cell wall metabolism, membrane lipid metabolism, and soluble sugar metabolism. Taken together, up-regulation of N-glycosylation, particularly complex-type N-glycosylation, as cellular responses to enhance chilling resistance may provide novel molecular insights and thus potential post-harvest solutions.
{"title":"Integrative N-glycomics and N-glycoproteomics landscape reveals up-regulation of complex-type N-glycosylation in the tolerance of peach fruit to chilling injury","authors":"Huiwen Wang , Tonghao Cui , Jiaqi Wang , Ruohan Ou , Yilong Liu , Bo Zhang , Changjie Xu , Changqing Zhu , Miaojin Chen , Yanna Shi , Yuanjiang Pan , Kunsong Chen , Xiaoyong Zhao , Xian Li","doi":"10.1016/j.postharvbio.2025.113433","DOIUrl":"10.1016/j.postharvbio.2025.113433","url":null,"abstract":"<div><div>Post-harvest chilling injury (CI) is a severe problem that causes great economic losses. The peach is an important fruit consumed worldwide but susceptible to CI under post-harvest storage, and thus serves as the typical model for CI research. Protein N-glycosylation plays a vital role under abiotic stress in plants. Here, a CI-associated up-regulation of N-glycosylation was observed in peach fruit stored at 5 ℃. An integrative N-glycomics and N-glycoproteomics analysis by reactive matrix-assisted laser desorption ionization time-of-flight mass spectrometry and <sup>18</sup>O-labeled tagging with nano-liquid chromatography-electrospray ionization tandem mass spectrometry, respectively, was developed to provide first insights into N-glycosylation remodeling for fruit tolerance to CI. On one side, 27 N-glycans were identified by N-glycomics analysis and seven complex-type N-glycans were substantially up-regulated in CI group. On the other side, N-glycoproteomics analysis identified 1002 N-glycosites on 732 N-glycoproteins. Differential and gene set analyses revealed 88 common N-glycoproteins, based on which three KEGG pathways including metabolic pathways, amino sugar & nucleotide sugar metabolism, and protein processing in endoplasmic reticulum (ER) were highly enriched. Among N-glycoproteins in top three enriched KEGG pathways, 29 N-glycosites were anchored and nearly 80 % of them were up-regulated in CI group. The corresponding 22 hub N-glycoproteins were primarily involved in CI-related biological processes such as phytohormone signaling, redox homeostasis, biotic stress, ER quality control, cell wall metabolism, membrane lipid metabolism, and soluble sugar metabolism. Taken together, up-regulation of N-glycosylation, particularly complex-type N-glycosylation, as cellular responses to enhance chilling resistance may provide novel molecular insights and thus potential post-harvest solutions.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113433"},"PeriodicalIF":6.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-10DOI: 10.1016/j.postharvbio.2025.113442
Qi Zheng , Wenhui Tian , Shanshan Wang , Zhijun Chen , Haihong Wang , Ling Yue , Weiqiang Yan , Wenyuan Qi , Ci Zhang , Xiaoyan Xu , Qiulian Kong
Rapid postharvest softening, a major issue with Actinidia arguta, leads to a decline in quality and in commercial value. To maximally preserve fruit quality, the effects of different doses of electron beam irradiation (0.4 kGy and 1 kGy) on fruit softening and the antioxidant capacity of A. arguta after 30 days of storage were investigated. The results indicated that, compared to unirradiated fruits, A. arguta fruit subjected to electron beam irradiation maintained high levels of firmness, total phenols, flavonoids, protopectin, starch, and sucrose, and exhibited low levels of malondialdehyde and reducing sugars. Fruit receiving 1 kGy irradiation exhibited the highest firmness; however, a significant reduction in firmness was observed immediately after irradiation (day 0). An irradiation dose of 0.4 kGy did not decrease fruit firmness, and there were no significant differences in the contents of protopectin, starch, sucrose, total phenols, and flavonoids compared to fruits treated with 1 kGy irradiation. A transcriptomic analysis revealed that electron beam irradiation significantly downregulated the most genes associated with the cell wall and starch degradation, and significantly upregulated some genes related to antioxidant activity. Furthermore, there was a positive correlation between the upregulated and downregulated gene expression levels and the radiation dose. The unigenes obtained from the transcriptomic analysis were validated through integrated RT-qPCR, proteomic, and transcriptomic correlation analyses. Overall, these findings indicated that electron beam irradiation is an effective method for delaying the softening of A. arguta.
{"title":"Electron beam irradiation maintains postharvest quality of Actinidia arguta by regulating the cell wall, starch degradation, and antioxidant capacity","authors":"Qi Zheng , Wenhui Tian , Shanshan Wang , Zhijun Chen , Haihong Wang , Ling Yue , Weiqiang Yan , Wenyuan Qi , Ci Zhang , Xiaoyan Xu , Qiulian Kong","doi":"10.1016/j.postharvbio.2025.113442","DOIUrl":"10.1016/j.postharvbio.2025.113442","url":null,"abstract":"<div><div>Rapid postharvest softening, a major issue with <em>Actinidia arguta</em>, leads to a decline in quality and in commercial value. To maximally preserve fruit quality, the effects of different doses of electron beam irradiation (0.4 kGy and 1 kGy) on fruit softening and the antioxidant capacity of <em>A. arguta</em> after 30 days of storage were investigated. The results indicated that, compared to unirradiated fruits, <em>A. arguta</em> fruit subjected to electron beam irradiation maintained high levels of firmness, total phenols, flavonoids, protopectin, starch, and sucrose, and exhibited low levels of malondialdehyde and reducing sugars. Fruit receiving 1 kGy irradiation exhibited the highest firmness; however, a significant reduction in firmness was observed immediately after irradiation (day 0). An irradiation dose of 0.4 kGy did not decrease fruit firmness, and there were no significant differences in the contents of protopectin, starch, sucrose, total phenols, and flavonoids compared to fruits treated with 1 kGy irradiation. A transcriptomic analysis revealed that electron beam irradiation significantly downregulated the most genes associated with the cell wall and starch degradation, and significantly upregulated some genes related to antioxidant activity. Furthermore, there was a positive correlation between the upregulated and downregulated gene expression levels and the radiation dose. The unigenes obtained from the transcriptomic analysis were validated through integrated RT-qPCR, proteomic, and transcriptomic correlation analyses. Overall, these findings indicated that electron beam irradiation is an effective method for delaying the softening of <em>A. arguta</em>.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113442"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-10DOI: 10.1016/j.postharvbio.2025.113441
Suong Tuyet Thi Ha, Ji Yeong Ham, Yong-Tae Kim, Byung-Chun In
Botrytis cinerea (B. cinerea), a major postharvest disease-causing fungus, causes considerable ornamental and economic losses in cut flowers, but the mechanisms by which phytohormones modulate B. cinerea resistance in cut flowers remain unclear. Here, we explored the role of plant hormones including ethylene (ETH), jasmonic acid (JA), and salicylic acid (SA), in the response to B. cinerea infection in rose cultivars with different ETH sensitivities. The results showed that ETH-highly sensitive (EthHS) cultivars exhibited faster disease progression and more severe symptoms of gray mold disease (GMD) symptoms than ETH-low sensitive (EthLS) cultivars when exposed to B. cinerea and ETH. B. cinerea infection significantly altered the expression patterns of ETH, JA, and SA biosynthesis and the signaling genes in rose petals. Notably, the activation of ETH- and SA-related genes by the GMD infection was closely related to the ETH sensitivity of the petal tissues, while JA signaling functioned independently. ETH and SA treatments increased the GMD susceptibility by upregulating B. cinerea histidine kinase receptors and pathogenicity-related genes, thereby accelerating tissue senescence in EthHS roses with or without B. cinerea infection. Contrastingly, the EthLS flowers treated with ETH and SA showed elevated GMD symptoms only when inoculated with fungi. Methyl JA (MeJA) treatment reduced the GMD severity and downregulated the transcription of B. cinerea-related genes, regardless of the tissue sensitivity to ETH. A working model was established to illustrate the antagonistic roles of ETH and SA against JA in regulating B. cinerea resistance in cut roses. These insights into hormone crosstalk provide potential strategies for enhancing B. cinerea resistance in cut rose flowers.
{"title":"Interaction between plant hormones in response to Botrytis cinerea infection in cut flowers with differential tissue sensitivity to ethylene","authors":"Suong Tuyet Thi Ha, Ji Yeong Ham, Yong-Tae Kim, Byung-Chun In","doi":"10.1016/j.postharvbio.2025.113441","DOIUrl":"10.1016/j.postharvbio.2025.113441","url":null,"abstract":"<div><div><em>Botrytis cinerea</em> (<em>B. cinerea</em>), a major postharvest disease-causing fungus, causes considerable ornamental and economic losses in cut flowers, but the mechanisms by which phytohormones modulate <em>B. cinerea</em> resistance in cut flowers remain unclear. Here, we explored the role of plant hormones including ethylene (ETH), jasmonic acid (JA), and salicylic acid (SA), in the response to <em>B. cinerea</em> infection in rose cultivars with different ETH sensitivities. The results showed that ETH-highly sensitive (EthHS) cultivars exhibited faster disease progression and more severe symptoms of gray mold disease (GMD) symptoms than ETH-low sensitive (EthLS) cultivars when exposed to <em>B. cinerea</em> and ETH. <em>B. cinerea</em> infection significantly altered the expression patterns of ETH, JA, and SA biosynthesis and the signaling genes in rose petals. Notably, the activation of ETH- and SA-related genes by the GMD infection was closely related to the ETH sensitivity of the petal tissues, while JA signaling functioned independently. ETH and SA treatments increased the GMD susceptibility by upregulating <em>B. cinerea</em> histidine kinase receptors and pathogenicity-related genes, thereby accelerating tissue senescence in EthHS roses with or without <em>B. cinerea</em> infection. Contrastingly, the EthLS flowers treated with ETH and SA showed elevated GMD symptoms only when inoculated with fungi. Methyl JA (MeJA) treatment reduced the GMD severity and downregulated the transcription of <em>B. cinerea</em>-related genes, regardless of the tissue sensitivity to ETH. A working model was established to illustrate the antagonistic roles of ETH and SA against JA in regulating <em>B. cinerea</em> resistance in cut roses. These insights into hormone crosstalk provide potential strategies for enhancing <em>B. cinerea</em> resistance in cut rose flowers.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113441"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-10DOI: 10.1016/j.postharvbio.2025.113446
Xiaotong Li , Hongliang Li , Qingguo Wang , Song Zhang
Pak choi (Brassica rapa subsp. Chinensis) is a key vegetable in daily life, however, yellowing of its leaves poses a significant challenge during postharvest storage. Diacetyl, a naturally occurring substance, was found to delay postharvest leaf yellowing of pak choi in this research. Additionally, the regulatory mechanism associated with diacetyl-mediated inhibition of leaf yellowing in pak choi was elucidated. We observed that diacetyl (7 μL L–1) delayed the leaf yellowing and suppressed chlorophyll degradation related enzyme activities. Various chlorophyll degradation related genes were also repressed following diacetyl treatment. Transcriptome analysis identified numerous differentially expressed genes (DEGs) in diacetyl-treated group compared to control group. Analysis of DEGs revealed that diacetyl significantly regulated genes involved in abscisic acid (ABA) biosynthesis and the ABA signaling pathway. Furthermore, diacetyl treatment inhibited ABA accumulation in pak choi. Importantly, a NAC (NAM, ATAF and CUC) transcription factor (TF), BcNAC100, which was repressed by diacetyl, was associated with leaf yellowing in pak choi. BcNAC100 was localized in nuclear and had high homology to ANAC100. Significantly, BcNAC100 can bind to the promoters of BcNCED3 (nine-cis-epoxycarotenoid dioxygenase 3) and BcZEP (zeaxanthin epoxidase) genes, positively regulating their expression to enhance ABA synthesis. These results demonstrated that diacetyl repressed BcNAC100 expression, reducing ABA accumulation and delaying leaf yellowing in pak choi. Furthermore, these findings revealed a novel molecular regulatory pathway in regulating leaf yellowing of pak choi and identified diacetyl as a potential vegetable preservation agent.
{"title":"Diacetyl delays postharvest leaf yellowing of pak choi (Brassica rapa subsp. Chinensis) by inhibiting ABA synthesis","authors":"Xiaotong Li , Hongliang Li , Qingguo Wang , Song Zhang","doi":"10.1016/j.postharvbio.2025.113446","DOIUrl":"10.1016/j.postharvbio.2025.113446","url":null,"abstract":"<div><div>Pak choi (<em>Brassica rapa</em> subsp. <em>Chinensis</em>) is a key vegetable in daily life, however, yellowing of its leaves poses a significant challenge during postharvest storage. Diacetyl, a naturally occurring substance, was found to delay postharvest leaf yellowing of pak choi in this research. Additionally, the regulatory mechanism associated with diacetyl-mediated inhibition of leaf yellowing in pak choi was elucidated. We observed that diacetyl (7 μL L<sup>–1</sup>) delayed the leaf yellowing and suppressed chlorophyll degradation related enzyme activities. Various chlorophyll degradation related genes were also repressed following diacetyl treatment. Transcriptome analysis identified numerous differentially expressed genes (DEGs) in diacetyl-treated group compared to control group. Analysis of DEGs revealed that diacetyl significantly regulated genes involved in abscisic acid (ABA) biosynthesis and the ABA signaling pathway. Furthermore, diacetyl treatment inhibited ABA accumulation in pak choi. Importantly, a NAC (NAM, ATAF and CUC) transcription factor (TF), BcNAC100, which was repressed by diacetyl, was associated with leaf yellowing in pak choi. BcNAC100 was localized in nuclear and had high homology to ANAC100. Significantly, BcNAC100 can bind to the promoters of <em>BcNCED3</em> (nine-cis-epoxycarotenoid dioxygenase 3) and <em>BcZEP</em> (zeaxanthin epoxidase) genes, positively regulating their expression to enhance ABA synthesis. These results demonstrated that diacetyl repressed BcNAC100 expression, reducing ABA accumulation and delaying leaf yellowing in pak choi. Furthermore, these findings revealed a novel molecular regulatory pathway in regulating leaf yellowing of pak choi and identified diacetyl as a potential vegetable preservation agent.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113446"},"PeriodicalIF":6.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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