Abstract Introduction Periodontitis is a disease of the tissues surrounding the tooth, leading to tooth loss. Missing teeth are often restored with fixed prostheses supported by implants. The aim of this study was to analyze subgingival microbiota in periodontal pockets and around implants, as well as to assess the correlation of periodontal parameters and risk factors for periodontitis with the presence of selected bacteria. Materials and Methods The study included 33 patients of both sexes, aged 39 to 79 years, having their own dentition and intraosseous implants with healthy periodontium or reversible gingivitis. The patients underwent periodontal examination and bacteriological analysis with the use of a PET RT-PCR kit (MIP Pharma, Germany). Results Less plaque was observed on implant surfaces than around natural teeth (28.7% vs. 39.8%). On the other hand, significantly deeper pockets (2.9 vs. 2.3 mm), and a slightly higher gingival bleeding index (27.9 vs. 22.6%) were noted around implants. Conclusions There were no significant differences in the overall presence and number of studied pathogens between the natural teeth and implants. In the case of natural teeth, the presence of Porphyromonas gingivalis , Treponema denticola , Tannerella forsythia and Prevotella intermedia showed correlation with clinical status: the amount of plaque (Td, Tf), bleeding (Td, Tf, Pi) and probing depth (Pg, Td, Tf, Pi). Such relationships were not observed in the case of implants. Bacteria from periodontal pockets also demonstrated more correlation with the general condition compared to bacteria residing in tissues around implants.
牙周炎是一种牙齿周围组织的疾病，可导致牙齿脱落。缺失的牙齿通常用种植体支撑的固定假体修复。本研究的目的是分析牙周袋和种植体周围的牙龈下微生物群，并评估牙周参数和牙周炎危险因素与选定细菌存在的相关性。材料与方法本研究纳入33例患者，男女，年龄39 ~ 79岁，有自己的牙列和骨内种植体，牙周组织健康或可逆性牙龈炎。使用PET RT-PCR试剂盒(德国MIP制药公司)对患者进行牙周检查和细菌学分析。结果种植体表面菌斑明显少于天然牙(28.7% vs. 39.8%)。另一方面，种植体周围的口袋明显更深(2.9 vs 2.3 mm)，牙龈出血指数略高(27.9 vs 22.6%)。结论天然牙与种植牙的病原菌总体存在和数量无显著差异。在天然牙中，牙龈卟啉单胞菌、牙密螺旋体、连珠Tannerella forsythia和Prevotella intermedia的存在与临床状况相关:菌斑数量(Td, Tf)、出血(Td, Tf, Pi)和探探深度(Pg, Td, Tf, Pi)。在植入物的情况下没有观察到这种关系。与居住在种植体周围组织中的细菌相比，来自牙周袋的细菌也显示出与一般情况的更多相关性。
{"title":"The condition of periodontal tissues and selected microorganisms presence around implants and natural teeth","authors":"Magdalena Jaszczak-Małkowska, Ewa Grabowska, Bartłomiej Górski, Renata Górska","doi":"10.2478/ahem-2023-0009","DOIUrl":"https://doi.org/10.2478/ahem-2023-0009","url":null,"abstract":"Abstract Introduction Periodontitis is a disease of the tissues surrounding the tooth, leading to tooth loss. Missing teeth are often restored with fixed prostheses supported by implants. The aim of this study was to analyze subgingival microbiota in periodontal pockets and around implants, as well as to assess the correlation of periodontal parameters and risk factors for periodontitis with the presence of selected bacteria. Materials and Methods The study included 33 patients of both sexes, aged 39 to 79 years, having their own dentition and intraosseous implants with healthy periodontium or reversible gingivitis. The patients underwent periodontal examination and bacteriological analysis with the use of a PET RT-PCR kit (MIP Pharma, Germany). Results Less plaque was observed on implant surfaces than around natural teeth (28.7% vs. 39.8%). On the other hand, significantly deeper pockets (2.9 vs. 2.3 mm), and a slightly higher gingival bleeding index (27.9 vs. 22.6%) were noted around implants. Conclusions There were no significant differences in the overall presence and number of studied pathogens between the natural teeth and implants. In the case of natural teeth, the presence of Porphyromonas gingivalis , Treponema denticola , Tannerella forsythia and Prevotella intermedia showed correlation with clinical status: the amount of plaque (Td, Tf), bleeding (Td, Tf, Pi) and probing depth (Pg, Td, Tf, Pi). Such relationships were not observed in the case of implants. Bacteria from periodontal pockets also demonstrated more correlation with the general condition compared to bacteria residing in tissues around implants.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135402332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ewa Malwina Milewska-Kobos, Ewelina Szczepanek-Parulska, Marek Ruchala
Abstract Obesity is a chronic, relapsing, and progressive disease that leads to negative health consequences. Excessive adiposity frequently coexists with metabolic and nonmetabolic complications, deteriorating health and reducing quality of life and life span. Individuals with obesity are not a homogenous group and can present different obesity phenotypes. The most common obesity phenotypes include: metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO). The latter category involves those with developed metabolic syndrome (MetS) and non-fully-developed metabolic syndrome (pre-MetS). The fundamental factor leading to obesity is imbalance between energy uptake and expenditure. From this perspective the thyroid gland plays the pivotal role in metabolism regulation and obesity development. The thyroid regulates thermogenesis, appetite, and lipids turnover. Clinically, hypothyroid patients have decreased metabolic rate and subsequently experience increase of BMI and excess adiposity. The interaction between the thyroid gland and obesity is bidirectional. Several mechanisms of alteration of the hypothalamus-pituitary-thyroid axis in obesity are proposed. Excessive adiposity and dysfunction of adipose tissue may contribute to the development of thyroid functional and structural impairment, such as autoimmunity, thyroid nodules, and thyroid cancer. The prevalence of certain thyroid disorders in obese individuals is higher than in nonobese subjects and this trend is more pronounced in unhealthy obesity phenotypes. The aim of this mini-review is to present the current knowledge on the interaction between thyroid gland disorders and obesity, with special focus on obesity phenotypes.
{"title":"Is there a link between obesity phenotype and thyroid diseases? A mini-review of current concepts","authors":"Ewa Malwina Milewska-Kobos, Ewelina Szczepanek-Parulska, Marek Ruchala","doi":"10.2478/ahem-2023-0015","DOIUrl":"https://doi.org/10.2478/ahem-2023-0015","url":null,"abstract":"Abstract Obesity is a chronic, relapsing, and progressive disease that leads to negative health consequences. Excessive adiposity frequently coexists with metabolic and nonmetabolic complications, deteriorating health and reducing quality of life and life span. Individuals with obesity are not a homogenous group and can present different obesity phenotypes. The most common obesity phenotypes include: metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO). The latter category involves those with developed metabolic syndrome (MetS) and non-fully-developed metabolic syndrome (pre-MetS). The fundamental factor leading to obesity is imbalance between energy uptake and expenditure. From this perspective the thyroid gland plays the pivotal role in metabolism regulation and obesity development. The thyroid regulates thermogenesis, appetite, and lipids turnover. Clinically, hypothyroid patients have decreased metabolic rate and subsequently experience increase of BMI and excess adiposity. The interaction between the thyroid gland and obesity is bidirectional. Several mechanisms of alteration of the hypothalamus-pituitary-thyroid axis in obesity are proposed. Excessive adiposity and dysfunction of adipose tissue may contribute to the development of thyroid functional and structural impairment, such as autoimmunity, thyroid nodules, and thyroid cancer. The prevalence of certain thyroid disorders in obese individuals is higher than in nonobese subjects and this trend is more pronounced in unhealthy obesity phenotypes. The aim of this mini-review is to present the current knowledge on the interaction between thyroid gland disorders and obesity, with special focus on obesity phenotypes.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"2015 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135318104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aneta Ścieżyńska, Anna Sobiepanek, Marta Soszyńska, Krzysztof Łuszczyński, Marcin Radziszewski, Iryna Levkovych, Natalia Krześniak, Beata Orzechowska, Anna Lutyńska, Jacek Malejczyk
Abstract Introduction Development of an effective, suitable, and reliable method for both the isolation and culturing of melanocytes is crucial for studies on pathomechanisms of skin diseases originating from melanocyte disorders. In this study, we have investigated the influence of geneticin (G418), a substance used for melanocyte selection, in the view of the frequency of presence of cells such as keratinocytes and fibroblasts, widely known as contaminators of melanocyte-originating cell cultures. Materials and Methods Study was conducted on primary, freshly isolated melanocytes, keratinocytes, fibroblasts, and melanoma cells as well as on commercially available melanoma cell lines MeWo, G-361, and A375. Cells were cultured in different culture media supplemented with various concentrations of geneticin ranging from 0.05 to 1 mg/mL. Cell viability, proliferation rate and detection of apoptotic/necrotic cells was assessed. Results Choice of culture media supplemented with various concentrations of geneticin (0.05 mg/mL, 0.1 mg/mL, 0.5 mg/mL and 1 mg/mL) strongly affect viability of melanocytes, fibroblasts, and keratinocytes. Selective culture media without FBS facilitate the process of melanocytes and melanoma pure cell culture, yet without geneticin supplementation are insufficient for complete eradication of fibroblast contamination from cell culture. Conclusions In this study we provide, for the first time, the dose-response action of keratinocytes and fibroblasts upon geneticin stimulation in different culture media and show that a low concentration (0.05 mg/mL) of geneticin added to the selective culture media may be safely implemented to facilitate the production of melanocyte and melanoma cell cultures that are free from frequent cell contaminants.
{"title":"Role of geneticin in isolation and culturing of skin melanocytes and melanoma cells","authors":"Aneta Ścieżyńska, Anna Sobiepanek, Marta Soszyńska, Krzysztof Łuszczyński, Marcin Radziszewski, Iryna Levkovych, Natalia Krześniak, Beata Orzechowska, Anna Lutyńska, Jacek Malejczyk","doi":"10.2478/ahem-2023-0014","DOIUrl":"https://doi.org/10.2478/ahem-2023-0014","url":null,"abstract":"Abstract Introduction Development of an effective, suitable, and reliable method for both the isolation and culturing of melanocytes is crucial for studies on pathomechanisms of skin diseases originating from melanocyte disorders. In this study, we have investigated the influence of geneticin (G418), a substance used for melanocyte selection, in the view of the frequency of presence of cells such as keratinocytes and fibroblasts, widely known as contaminators of melanocyte-originating cell cultures. Materials and Methods Study was conducted on primary, freshly isolated melanocytes, keratinocytes, fibroblasts, and melanoma cells as well as on commercially available melanoma cell lines MeWo, G-361, and A375. Cells were cultured in different culture media supplemented with various concentrations of geneticin ranging from 0.05 to 1 mg/mL. Cell viability, proliferation rate and detection of apoptotic/necrotic cells was assessed. Results Choice of culture media supplemented with various concentrations of geneticin (0.05 mg/mL, 0.1 mg/mL, 0.5 mg/mL and 1 mg/mL) strongly affect viability of melanocytes, fibroblasts, and keratinocytes. Selective culture media without FBS facilitate the process of melanocytes and melanoma pure cell culture, yet without geneticin supplementation are insufficient for complete eradication of fibroblast contamination from cell culture. Conclusions In this study we provide, for the first time, the dose-response action of keratinocytes and fibroblasts upon geneticin stimulation in different culture media and show that a low concentration (0.05 mg/mL) of geneticin added to the selective culture media may be safely implemented to facilitate the production of melanocyte and melanoma cell cultures that are free from frequent cell contaminants.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"269 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135442129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Hypertension is still the cause of many cardiovascular complications and the premature death of a large number of patients despite the use of increasingly effective pharmacological drugs. Most patients with therapy-resistant hypertension show excessive activity of the sympathetic system. In the last two decades, there have been successful attempts at nonpharmacological reduction of this activity. The earliest method for this purpose is the denervation of the renal arteries by using devices specially designed for this purpose. Other methods of lowering blood pressure are the electrical activation of baroreceptors, which increases the tone of the parasympathetic system, and special pacemaker software to lower blood pressure. The paper discusses the studies assessing the effectiveness and safety of these methods, and on the basis of the current research results, predictions are made concerning which patients with arterial hypertension would be the most appropriate for these treatment methods.
{"title":"Devices for the treatment of arterial hypertension","authors":"J. Głuszek, T. Kosicka","doi":"10.2478/ahem-2023-0004","DOIUrl":"https://doi.org/10.2478/ahem-2023-0004","url":null,"abstract":"Abstract Hypertension is still the cause of many cardiovascular complications and the premature death of a large number of patients despite the use of increasingly effective pharmacological drugs. Most patients with therapy-resistant hypertension show excessive activity of the sympathetic system. In the last two decades, there have been successful attempts at nonpharmacological reduction of this activity. The earliest method for this purpose is the denervation of the renal arteries by using devices specially designed for this purpose. Other methods of lowering blood pressure are the electrical activation of baroreceptors, which increases the tone of the parasympathetic system, and special pacemaker software to lower blood pressure. The paper discusses the studies assessing the effectiveness and safety of these methods, and on the basis of the current research results, predictions are made concerning which patients with arterial hypertension would be the most appropriate for these treatment methods.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"77 1","pages":"27 - 35"},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69111692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lei Tan, Qi-zhong Fan, Yuegao Chen, Hao Wu, Huanxi Xu, M. Zhong, Lei Wu
Abstract Introduction We sought out to evaluate the effects of oxycodone hydrochloride injection on laparoscopic cholecystectomy (LC), substance P, 5-hydroxytryptamine (5-HT), and patient-controlled intravenous analgesia (PCIA). Materials and Methods A total of 120 eligible patients were randomly divided into an observation group (n=60) and control group (n=60). Oxycodone (0.07 mg/kg) and fentanyl (0.7 μg/kg) were intravenously injected during LC. Substance P and 5-HT were detected by ELISA. Results Heart rate (HR), mean arterial pressure (MAP), and blood oxygen saturation (SpO2) were recorded. Visual analogue scale (VAS), Ramsay scores, and incidence rates of adverse reactions were recorded. The observation group was less prone to agitation, with less affected HR, MAP and SpO2 during recovery. Before and after extubation, and upon leaving the recovery room, substance P and 5-HT increased more slowly in the observation group. After extubation, the control group had higher VAS and Ramsay scores and a higher incidence rate of adverse reactions. Using oxycodone before the end of LC does not delay awakening or affect extubation, but reduces hemodynamic fluctuations, incidence of agitation and release of substance P and 5-HT. It has high safety and stable circulation during recovery. Conclusions Oxycodone used in PCIA after LC effectively mitigates postoperative pain, with milder adverse reactions, which is superior to fentanyl.
{"title":"Analgesic effects of oxycodone hydrochloride injection after laparoscopic cholecystectomy and influence on substance P, 5-hydroxytryptamine, and patient-controlled intravenous analgesia","authors":"Lei Tan, Qi-zhong Fan, Yuegao Chen, Hao Wu, Huanxi Xu, M. Zhong, Lei Wu","doi":"10.2478/ahem-2023-0006","DOIUrl":"https://doi.org/10.2478/ahem-2023-0006","url":null,"abstract":"Abstract Introduction We sought out to evaluate the effects of oxycodone hydrochloride injection on laparoscopic cholecystectomy (LC), substance P, 5-hydroxytryptamine (5-HT), and patient-controlled intravenous analgesia (PCIA). Materials and Methods A total of 120 eligible patients were randomly divided into an observation group (n=60) and control group (n=60). Oxycodone (0.07 mg/kg) and fentanyl (0.7 μg/kg) were intravenously injected during LC. Substance P and 5-HT were detected by ELISA. Results Heart rate (HR), mean arterial pressure (MAP), and blood oxygen saturation (SpO2) were recorded. Visual analogue scale (VAS), Ramsay scores, and incidence rates of adverse reactions were recorded. The observation group was less prone to agitation, with less affected HR, MAP and SpO2 during recovery. Before and after extubation, and upon leaving the recovery room, substance P and 5-HT increased more slowly in the observation group. After extubation, the control group had higher VAS and Ramsay scores and a higher incidence rate of adverse reactions. Using oxycodone before the end of LC does not delay awakening or affect extubation, but reduces hemodynamic fluctuations, incidence of agitation and release of substance P and 5-HT. It has high safety and stable circulation during recovery. Conclusions Oxycodone used in PCIA after LC effectively mitigates postoperative pain, with milder adverse reactions, which is superior to fentanyl.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"77 1","pages":"42 - 48"},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69111733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Krawczyk, Katarzyna Talaga-Ćwiertnia, Katarzyna Biegun, Kamil Drożdż, D. Salamon, T. Gosiewski, Agnieszka Sroka-Oleksiak
Abstract Introduction There is evidence of the existence of quantitative changes in the microbiome, including Bifidobacterium spp., due to some chronic diseases, such as liver cirrhosis, inflammatory bowel diseases, obesity, or celiac disease. Materials and Methods We aimed to examine the number of Bifidobacterium and total bacteria present in the colon of patients with type 1 diabetes (T1DM) and type 2 diabetes (T2DM), as well as in healthy subjects. DNA was extracted from patients’ fecal samples and then amplified by real-time PCR to determine the number of Bifidobacterium and total bacteria. Statistical association with selected clinical and biochemical features was examined. Results The mean numbers of bacteria belonging to the genus Bifidobacterium in T1DM and T2DM were lower compared to the control group (p = 0.006, p < 0.001 respectively). There were no statistical differences in the total number of bacteria between all groups (p = 0.397). In the T1DM group, a significant correlation was detected between the number of bifidobacteria and age (r = 0.441, p = 0.010), as well as bifidobacteria and alanine aminotransferease (p = 0.022, r = −0.11). In the group T2DM, a correlation was observed between triglycerydes and bifidobacteria (p < 0.001, r = −0.61). Moreover, we have found a negative correlation between HBA1, glucose level, and bifidobacteria (r = −0.35, p < 0.001 and r = −0.024, p = 0.019, respectively). Conclusions The quantitative composition of Bifidobacterium is lower in T1DM and T2DM patients compared to the healthy controls. Further studies are needed to clarify the relationship between the number of these bacteria and elements of the clinical picture of T1DM.
有证据表明，由于一些慢性疾病，如肝硬化、炎症性肠病、肥胖或乳糜泻，包括双歧杆菌在内的微生物组存在定量变化。材料和方法我们旨在检测1型糖尿病(T1DM)和2型糖尿病(T2DM)患者以及健康受试者结肠中双歧杆菌和总细菌的数量。从患者粪便样本中提取DNA，通过实时荧光定量PCR扩增，测定双歧杆菌数量和总菌数。统计关联与选定的临床和生化特征进行了检查。结果T1DM和T2DM患者双歧杆菌属细菌的平均数量低于对照组(p = 0.006, p < 0.001)。各组细菌总数比较差异无统计学意义(p = 0.397)。在T1DM组中，双歧杆菌数量与年龄(r = 0.441, p = 0.010)、双歧杆菌数量与丙氨酸氨基转移酶(p = 0.022, r = - 0.11)显著相关。在T2DM组中，甘油三酯和双歧杆菌之间存在相关性(p < 0.001, r = - 0.61)。此外，我们还发现HBA1、葡萄糖水平和双歧杆菌之间存在负相关(r = - 0.35, p < 0.001和r = - 0.024, p = 0.019)。结论T1DM和T2DM患者双歧杆菌的定量组成低于健康对照组。需要进一步的研究来阐明这些细菌的数量与T1DM临床表现要素之间的关系。
{"title":"Quantitative evaluation of the genus Bifidobacterium in stool samples of patients with type 1 and 2 diabetes","authors":"A. Krawczyk, Katarzyna Talaga-Ćwiertnia, Katarzyna Biegun, Kamil Drożdż, D. Salamon, T. Gosiewski, Agnieszka Sroka-Oleksiak","doi":"10.2478/ahem-2023-0007","DOIUrl":"https://doi.org/10.2478/ahem-2023-0007","url":null,"abstract":"Abstract Introduction There is evidence of the existence of quantitative changes in the microbiome, including Bifidobacterium spp., due to some chronic diseases, such as liver cirrhosis, inflammatory bowel diseases, obesity, or celiac disease. Materials and Methods We aimed to examine the number of Bifidobacterium and total bacteria present in the colon of patients with type 1 diabetes (T1DM) and type 2 diabetes (T2DM), as well as in healthy subjects. DNA was extracted from patients’ fecal samples and then amplified by real-time PCR to determine the number of Bifidobacterium and total bacteria. Statistical association with selected clinical and biochemical features was examined. Results The mean numbers of bacteria belonging to the genus Bifidobacterium in T1DM and T2DM were lower compared to the control group (p = 0.006, p < 0.001 respectively). There were no statistical differences in the total number of bacteria between all groups (p = 0.397). In the T1DM group, a significant correlation was detected between the number of bifidobacteria and age (r = 0.441, p = 0.010), as well as bifidobacteria and alanine aminotransferease (p = 0.022, r = −0.11). In the group T2DM, a correlation was observed between triglycerydes and bifidobacteria (p < 0.001, r = −0.61). Moreover, we have found a negative correlation between HBA1, glucose level, and bifidobacteria (r = −0.35, p < 0.001 and r = −0.024, p = 0.019, respectively). Conclusions The quantitative composition of Bifidobacterium is lower in T1DM and T2DM patients compared to the healthy controls. Further studies are needed to clarify the relationship between the number of these bacteria and elements of the clinical picture of T1DM.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"77 1","pages":"59 - 64"},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69111750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kacper Deska, Bartłomiej Bąk, Marcin Kosmalski, T. Pietras
Abstract Type two diabetes has become a civilization disease in the recent years, and the accompanying obesity, metabolic syndrome and non-alcoholic fatty liver are often the inseparable components of the clinical presentation in patients with diabetes of this type. The treatment of each of these elements is important for optimal metabolic control of the patients, as well as directly affecting their life expectancy. However, The ideal solution would be to take as few drugs as possible, preferably drugs that have a beneficial effect on several coexisting diseases at the same time. In the recent years, there have been more and more reports about the pleiotropic effect of drugs affecting the incretin axis - GLP-1 analogues. The presented paper provides an overview of the latest knowledge on the effect of GLP-1 receptor agonists on weight reduction and reduction of changes in the course of non-alcoholic fatty liver disease.
{"title":"GLP-1 analogues in the treatment of obesity and non-alcoholic fatty liver disease","authors":"Kacper Deska, Bartłomiej Bąk, Marcin Kosmalski, T. Pietras","doi":"10.2478/ahem-2023-0001","DOIUrl":"https://doi.org/10.2478/ahem-2023-0001","url":null,"abstract":"Abstract Type two diabetes has become a civilization disease in the recent years, and the accompanying obesity, metabolic syndrome and non-alcoholic fatty liver are often the inseparable components of the clinical presentation in patients with diabetes of this type. The treatment of each of these elements is important for optimal metabolic control of the patients, as well as directly affecting their life expectancy. However, The ideal solution would be to take as few drugs as possible, preferably drugs that have a beneficial effect on several coexisting diseases at the same time. In the recent years, there have been more and more reports about the pleiotropic effect of drugs affecting the incretin axis - GLP-1 analogues. The presented paper provides an overview of the latest knowledge on the effect of GLP-1 receptor agonists on weight reduction and reduction of changes in the course of non-alcoholic fatty liver disease.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"77 1","pages":"1 - 8"},"PeriodicalIF":0.3,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69111764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joanna Dominika Waś, Magdalena Niedolistek, Dorota Sokołowska, Joanna Wiśniewska, Tomasz Zieliński, Anna Mierzyńska, Daniel Rabczenko, Beata Orzechowska, Dominika Franz, Ewa Augustynowicz-Kopeć, Anna Lutyńska
Abstract Introduction Cardiovascular diseases (CVDs) are the leading cause of death in the world. The dose of 60 g of alcohol / day has been estimated to significantly increase the risk of developing high blood pressure, arrhythmias, hemorrhagic stroke, and cardiomyopathy. The aim of the study was to evaluate the diagnostic value of CDT when compared with gammaglutamyltransferase (GGT), alkaline phosphatase (ALP), N-terminal fragment brain natriuretic peptide (NT pro-BNP) and troponin T biomarkers in patients with heart failure. Materials and Methods The study was conducted on 59 patients with advanced heart failure with reduced ejection fraction (EF<40%). CDT serum levels were measured using CDT kit, Chromsystems Instruments & Chemicals GmbH, Germany and were expressed as a percentage of total transferrin using high-performance liquid chromatography (HPLC). Results Nineteen patients (n = 19) expressed a normal level of carbohydrate deficiency transferrin <1.2% of the total transferrin found in plasma. The results are obtained from 34 patients (n = 34) were within the range doubtful for the test (1.2–2.5%). In 6 Patients (n = 6) the results of more than 2.5% of CDT content were observed, which could indicate chronic alcohol consumption. Conclusions No statistically significant correlations between CDT and troponin T or NT pro-BNP were identified.
{"title":"The carbohydrate-deficient transferrin as a marker of chronic alcohol consumption and assessment of its usefulness in patients with cardiovascular diseases – a pilot study","authors":"Joanna Dominika Waś, Magdalena Niedolistek, Dorota Sokołowska, Joanna Wiśniewska, Tomasz Zieliński, Anna Mierzyńska, Daniel Rabczenko, Beata Orzechowska, Dominika Franz, Ewa Augustynowicz-Kopeć, Anna Lutyńska","doi":"10.2478/ahem-2023-0010","DOIUrl":"https://doi.org/10.2478/ahem-2023-0010","url":null,"abstract":"Abstract Introduction Cardiovascular diseases (CVDs) are the leading cause of death in the world. The dose of 60 g of alcohol / day has been estimated to significantly increase the risk of developing high blood pressure, arrhythmias, hemorrhagic stroke, and cardiomyopathy. The aim of the study was to evaluate the diagnostic value of CDT when compared with gammaglutamyltransferase (GGT), alkaline phosphatase (ALP), N-terminal fragment brain natriuretic peptide (NT pro-BNP) and troponin T biomarkers in patients with heart failure. Materials and Methods The study was conducted on 59 patients with advanced heart failure with reduced ejection fraction (EF<40%). CDT serum levels were measured using CDT kit, Chromsystems Instruments & Chemicals GmbH, Germany and were expressed as a percentage of total transferrin using high-performance liquid chromatography (HPLC). Results Nineteen patients (n = 19) expressed a normal level of carbohydrate deficiency transferrin <1.2% of the total transferrin found in plasma. The results are obtained from 34 patients (n = 34) were within the range doubtful for the test (1.2–2.5%). In 6 Patients (n = 6) the results of more than 2.5% of CDT content were observed, which could indicate chronic alcohol consumption. Conclusions No statistically significant correlations between CDT and troponin T or NT pro-BNP were identified.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135954968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Introduction The present study aimed to compare the MIC values of colistin and polymyxin B in fermentative and non-fermentative Gram-negative bacteria and to examine whether the susceptibility results determined with colistin can be used for polymyxin B. Materials and Methods A total of 268 strains of Escherichia coli ( E. coli ), Klebsiella pneumoniae ( K. pneumoniae ), Pseudomonas aeruginosa ( P. aeruginosa ), and Acinetobacter baumannii ( A. baumannii ) isolates were included in the study. The reference broth microdilution and the results were evaluated according to CLSI recommendations. Results Of the 268 strains, 40.7% were found to be colistin resistant and 34% polymyxin B resistant. Essential agreement (EA) and categorical agreement (CA) ratios for all isolates were found to be 81.3% and 85.1%, respectively. Identical MIC values were found for colistin and polymyxin B in 104 isolates (38.8%), and this rate was 31.1% and 48.7% in Enterobacterales and non-fermentative bacteria, respectively. Colistin resistance rates were 25.4% for E. coli , 67.4% for K. pneumoniae , 23.8% for P. aeruginosa , and 31.5% for A. baumannii . Polymyxin B resistance rates were 18.6% for E. coli , 64.1% for K. pneumoniae , 19% for P. aeruginosa , and 16.7% for A. baumannii . Conclusion According to the results of the study, it was concluded that the MIC values of colistin and polymyxin B antibiotics in all bacteria were not compatible with each other. Colistin susceptibility results cannot be used for treatment with polymyxin B, despite CLSI's recommendation.
{"title":"Can colistin susceptibility results determined in multidrug resistant Gram-negative bacteria be used for polymyxin B?","authors":"Rıza Adaleti, Nilgün Kansak, Şeyma Çalık, Neslihan Arıcı, Seniha Şenbayrak, Sebahat Aksaray","doi":"10.2478/ahem-2023-0017","DOIUrl":"https://doi.org/10.2478/ahem-2023-0017","url":null,"abstract":"Abstract Introduction The present study aimed to compare the MIC values of colistin and polymyxin B in fermentative and non-fermentative Gram-negative bacteria and to examine whether the susceptibility results determined with colistin can be used for polymyxin B. Materials and Methods A total of 268 strains of Escherichia coli ( E. coli ), Klebsiella pneumoniae ( K. pneumoniae ), Pseudomonas aeruginosa ( P. aeruginosa ), and Acinetobacter baumannii ( A. baumannii ) isolates were included in the study. The reference broth microdilution and the results were evaluated according to CLSI recommendations. Results Of the 268 strains, 40.7% were found to be colistin resistant and 34% polymyxin B resistant. Essential agreement (EA) and categorical agreement (CA) ratios for all isolates were found to be 81.3% and 85.1%, respectively. Identical MIC values were found for colistin and polymyxin B in 104 isolates (38.8%), and this rate was 31.1% and 48.7% in Enterobacterales and non-fermentative bacteria, respectively. Colistin resistance rates were 25.4% for E. coli , 67.4% for K. pneumoniae , 23.8% for P. aeruginosa , and 31.5% for A. baumannii . Polymyxin B resistance rates were 18.6% for E. coli , 64.1% for K. pneumoniae , 19% for P. aeruginosa , and 16.7% for A. baumannii . Conclusion According to the results of the study, it was concluded that the MIC values of colistin and polymyxin B antibiotics in all bacteria were not compatible with each other. Colistin susceptibility results cannot be used for treatment with polymyxin B, despite CLSI's recommendation.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135704839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Drug-induced hypersensitivity syndrome (DIHS) is a potentially life-threatening reaction. The pathology of DIHS has been connected with adverse reactions to drugs; however, it has been observed that viruses and other infectious factors may induce similar responses in the body. COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has recently become a major challenge for worldwide healthcare. The dermatological symptoms occur in approximately 5.95% of COVID-19 patients, however, they are not exclusively a manifestation of SARS-CoV-2 infection. Certain skin-related symptoms might result from therapeutic drug administration, personal protective equipment, or sanitizers used by medical personnel. The complex pathomechanism of those symptoms leads to multiple diagnostic difficulties. This article describes a case of a 40-year-old man who was admitted to the intensive care unit (ICU) due to heavy respiratory failure in the course of SARS-CoV-2 infection. The patient has been treated with olanzapine for anxiety disorder for approximately 4 years. On the seventh day of treatment, the patient presented with a maculopapular rash, fever, and swollen upper lip. Because of these presented symptoms, the patient has been diagnosed with drug-induced hypersensitivity syndrome (DIHS). The results of blood analysis showed elevated levels of acute phase markers, liver and kidney damage markers, which are present both during COVID-19 and DIHS. Furthermore, the patient presented with peripheral eosinophilia, which is a distinctive feature of DIHS. In the course of COVID-19, the laboratory-measured levels of eosinophilic granulocytes declined to 0. Therefore, in the active phase of SARS-CoV-2 infection, the eosinophilia connected with DIHS was difficult to assess. In this paper, we compare and contrast the clinical and laboratory symptoms of COVID-19 and DIHS syndrome, describe patient's case and explain the diagnostics difficulties resulting from the concomitance of those disease entities.
{"title":"A patient infected with SARS-Cov-2 presents with DIHS syndrome induced by olanzapine: diagnostic difficulties","authors":"Michalina Pinkosz, Mateusz Szymański, Monika Cendrowska-Pinkosz","doi":"10.2478/ahem-2023-0016","DOIUrl":"https://doi.org/10.2478/ahem-2023-0016","url":null,"abstract":"Abstract Drug-induced hypersensitivity syndrome (DIHS) is a potentially life-threatening reaction. The pathology of DIHS has been connected with adverse reactions to drugs; however, it has been observed that viruses and other infectious factors may induce similar responses in the body. COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has recently become a major challenge for worldwide healthcare. The dermatological symptoms occur in approximately 5.95% of COVID-19 patients, however, they are not exclusively a manifestation of SARS-CoV-2 infection. Certain skin-related symptoms might result from therapeutic drug administration, personal protective equipment, or sanitizers used by medical personnel. The complex pathomechanism of those symptoms leads to multiple diagnostic difficulties. This article describes a case of a 40-year-old man who was admitted to the intensive care unit (ICU) due to heavy respiratory failure in the course of SARS-CoV-2 infection. The patient has been treated with olanzapine for anxiety disorder for approximately 4 years. On the seventh day of treatment, the patient presented with a maculopapular rash, fever, and swollen upper lip. Because of these presented symptoms, the patient has been diagnosed with drug-induced hypersensitivity syndrome (DIHS). The results of blood analysis showed elevated levels of acute phase markers, liver and kidney damage markers, which are present both during COVID-19 and DIHS. Furthermore, the patient presented with peripheral eosinophilia, which is a distinctive feature of DIHS. In the course of COVID-19, the laboratory-measured levels of eosinophilic granulocytes declined to 0. Therefore, in the active phase of SARS-CoV-2 infection, the eosinophilia connected with DIHS was difficult to assess. In this paper, we compare and contrast the clinical and laboratory symptoms of COVID-19 and DIHS syndrome, describe patient's case and explain the diagnostics difficulties resulting from the concomitance of those disease entities.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136047410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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