Abstract Introduction Otitis media with effusion (OME) is one of the most common otorhinolaryngological (ENT) disorders in childhood. This study aimed to investigate the vestibular organ condition in children with OME and whether the presence of vestibular disturbances depends on the advancement of OME. Materials and Methods Subjects were 53 children between 4 and 14 years old with bilateral OME and treated with middle ear drainage. The study group was divided into two subgroups according to the advancement of the disease. The participants were submitted to an evaluation consisting of anamnesis, ENT evaluation, static posturography, and electronystagmography (ENG). Examination was performed before surgery and one month after drainage. Results The posturography revealed the presence of disturbances before and after ME drainage. After drainage, the assessed parameters improved; they still, however, remained elevated. Disturbances in posturography, both before and after drainage, were expressed more fully in the subgroup with stage II compared to the stage I subgroup, especially before drainage. The analysis of the ENG confirmed that the stage of clinical advancement affects the severity of vestibular disorders in children with OME. Greater vestibular disorders in the form of the presence of spontaneous nystagmus and position were more frequent in the subgroup with stage II. Conclusions ME effusion affects the vestibular organ in children with OME. The degree of vestibular disturbances depends on the clinical advancement of the disease. The assessment of the vestibular organ condition is beneficial and should be included in the OME diagnostic and for qualification for surgical treatment.
{"title":"Impact of advancement of otitis media with effusion on vestibular organ condition in children","authors":"Katarzyna Pazdro-Zastawny, T. Zatoński","doi":"10.2478/ahem-2022-0041","DOIUrl":"https://doi.org/10.2478/ahem-2022-0041","url":null,"abstract":"Abstract Introduction Otitis media with effusion (OME) is one of the most common otorhinolaryngological (ENT) disorders in childhood. This study aimed to investigate the vestibular organ condition in children with OME and whether the presence of vestibular disturbances depends on the advancement of OME. Materials and Methods Subjects were 53 children between 4 and 14 years old with bilateral OME and treated with middle ear drainage. The study group was divided into two subgroups according to the advancement of the disease. The participants were submitted to an evaluation consisting of anamnesis, ENT evaluation, static posturography, and electronystagmography (ENG). Examination was performed before surgery and one month after drainage. Results The posturography revealed the presence of disturbances before and after ME drainage. After drainage, the assessed parameters improved; they still, however, remained elevated. Disturbances in posturography, both before and after drainage, were expressed more fully in the subgroup with stage II compared to the stage I subgroup, especially before drainage. The analysis of the ENG confirmed that the stage of clinical advancement affects the severity of vestibular disorders in children with OME. Greater vestibular disorders in the form of the presence of spontaneous nystagmus and position were more frequent in the subgroup with stage II. Conclusions ME effusion affects the vestibular organ in children with OME. The degree of vestibular disturbances depends on the clinical advancement of the disease. The assessment of the vestibular organ condition is beneficial and should be included in the OME diagnostic and for qualification for surgical treatment.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"60 1","pages":"300 - 306"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69110937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agnieszka Chrustek, E. Sinkiewicz-Darol, M. Lampka, D. Olszewska-Słonina, B. Sperkowska, K. Linowiecka
Abstract Introduction Women who have problems with lactation can use human milk banks. Mainly this human milk is provided to premature babies and sick newborns. Human milk is the most suitable food for newborns and infants, recommended by WHO (World Health Organization). Human milk has anti-inflammatory, anti-infective, and anti-allergic properties, and also works for immunomodulation. Melatonin has a special, underestimated importance in the composition of breast milk. It is a hormone that has many body functions and, for several decades, its antioxidant potential has been increasingly talked about. The aim of the study was to examine the effect of Holder pasteurization on melatonin concentration in human milk. Materials and Methods 18 samples of human milk from donors from the human milk bank were used for the analysis. Melatonin concentration before and after pasteurization was determined by ELISA. In addition, the nutritional content composition of milk was analyzed using MIRIS Human Milk Analyzer and correlations examined. Results Melatonin concentration in human milk before pasteurization was 0.65–26.24 pg/mL (Me=9.58, IQR=12.72), while after pasteurization 0.80–29.58 pg/mL (Me=9.98, IQR=11.26). There was a positive correlation between melatonin concentration before and after pasteurization (r=0.797, p<0.001). Conclusions The Holder pasteurization process does not affect the concentration of melatonin in milk samples, which may be a recommendation for human milk banks.
{"title":"Effect of pasteurization on melatonin concentration in human breast milk","authors":"Agnieszka Chrustek, E. Sinkiewicz-Darol, M. Lampka, D. Olszewska-Słonina, B. Sperkowska, K. Linowiecka","doi":"10.2478/ahem-2022-0022","DOIUrl":"https://doi.org/10.2478/ahem-2022-0022","url":null,"abstract":"Abstract Introduction Women who have problems with lactation can use human milk banks. Mainly this human milk is provided to premature babies and sick newborns. Human milk is the most suitable food for newborns and infants, recommended by WHO (World Health Organization). Human milk has anti-inflammatory, anti-infective, and anti-allergic properties, and also works for immunomodulation. Melatonin has a special, underestimated importance in the composition of breast milk. It is a hormone that has many body functions and, for several decades, its antioxidant potential has been increasingly talked about. The aim of the study was to examine the effect of Holder pasteurization on melatonin concentration in human milk. Materials and Methods 18 samples of human milk from donors from the human milk bank were used for the analysis. Melatonin concentration before and after pasteurization was determined by ELISA. In addition, the nutritional content composition of milk was analyzed using MIRIS Human Milk Analyzer and correlations examined. Results Melatonin concentration in human milk before pasteurization was 0.65–26.24 pg/mL (Me=9.58, IQR=12.72), while after pasteurization 0.80–29.58 pg/mL (Me=9.98, IQR=11.26). There was a positive correlation between melatonin concentration before and after pasteurization (r=0.797, p<0.001). Conclusions The Holder pasteurization process does not affect the concentration of melatonin in milk samples, which may be a recommendation for human milk banks.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"76 1","pages":"220 - 227"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47656158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Hepatic encephalopathy is a dysfunction of the central nervous system caused by chronic and acute liver disease. The dysfunction presents a wide spectrum of symptoms—from the undetectable in a standard clinical examination to hepatic coma—and could be caused by both chronic and acute liver diseases. For many years research has been conducted to find a marker that would allow for the accurate, quick, and possibly inexpensive detection of hepatic encephalopathy. Due to the pathogenesis of hepatic encephalopathy, researchers’ attention is focused on markers of damage to the central nervous system. One of the markers of astrocyte damage, known from research in neurology and neurosurgery, is the protein S100B. Published research results so far are inconclusive, but they allow us to look with optimism at the role of S100B as a marker of minimal hepatic encephalopathy (MHE).
{"title":"S100 Beta Protein as a Marker of Hepatic Encephalopathy: A Breakthrough in Diagnostics or a False Trail? Review of the Literature.","authors":"Wojciech Pisarek","doi":"10.2478/ahem-2022-0018","DOIUrl":"https://doi.org/10.2478/ahem-2022-0018","url":null,"abstract":"Abstract Hepatic encephalopathy is a dysfunction of the central nervous system caused by chronic and acute liver disease. The dysfunction presents a wide spectrum of symptoms—from the undetectable in a standard clinical examination to hepatic coma—and could be caused by both chronic and acute liver diseases. For many years research has been conducted to find a marker that would allow for the accurate, quick, and possibly inexpensive detection of hepatic encephalopathy. Due to the pathogenesis of hepatic encephalopathy, researchers’ attention is focused on markers of damage to the central nervous system. One of the markers of astrocyte damage, known from research in neurology and neurosurgery, is the protein S100B. Published research results so far are inconclusive, but they allow us to look with optimism at the role of S100B as a marker of minimal hepatic encephalopathy (MHE).","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"76 1","pages":"128 - 131"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69109981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Introduction Septic arthritis after arthroscopy (SAAA) is a devasting complication which reported frequency varies about 0.04%–5.7%. The aim of the study is to analyze frequency of SAAA at one orthopedic center, risk factors and accuracy of actual prophylactic measures. Materials and Methods A retrospective study (level of evidence: V) includes 794 (665 primary and 129 revision) “clean” arthroscopies performed in the years 2017–2018 with confirmed joint infection during 30 days after operation without the use of non-resorbable implants and up to 1 year after procedures with non-resorbable implants. Demographic and medical data about potential risk factors of SAAA were analyzed: patients age, sex, operated joint, type of procedure, primary or revision procedure, the use of drains, usage of non-absorbable implant, time of surgery, BMI, time lapse from index operation to diagnosis of SAAA, length of hospital stay, causative microorganisms. All operations were performed by eight different but experienced surgeons, in operation theatre with vertical laminar flow with the capacity of 50 air exchanges per hour. The perioperative antibiotic prophylaxis consisted of one preoperative dose 1 g cefazolin in simple arthroscopies, which was prolonged to 24 hours with 3 doses in cases of implantation of non-absorbable implant (55.8% of patients). Results From 794 cases 4 have been infected: 2 after knee arthrolysis, one after ACL reconstruction and one after rotator cuff repair. There were 2 early, with manifestation within 30 days, and 2 late-onset SAAA. Infections occurred in 0.5% of all arthroscopies and in 0.47% of knee arthroscopies alone. Patients age and time of surgery have not been found significantly different in infected and non-infected cases, whereas age and time of surgery have been significantly different in revision and non-revision cases. Conclusions Primarily aseptic arthroscopic procedures performed with respect to actual perioperative preventive measures have a low risk of postoperative septic arthritis. The risk increases with patients age and time of operation, but not significantly.
{"title":"Infection complicating 794 primary and revision arthroscopies. Accuracy of actual prophylactic procedures against infection and results from a single orthopedic center in Poland","authors":"Karolina Stępień, Karol Kosterna, I. Babiak","doi":"10.2478/ahem-2022-0026","DOIUrl":"https://doi.org/10.2478/ahem-2022-0026","url":null,"abstract":"Abstract Introduction Septic arthritis after arthroscopy (SAAA) is a devasting complication which reported frequency varies about 0.04%–5.7%. The aim of the study is to analyze frequency of SAAA at one orthopedic center, risk factors and accuracy of actual prophylactic measures. Materials and Methods A retrospective study (level of evidence: V) includes 794 (665 primary and 129 revision) “clean” arthroscopies performed in the years 2017–2018 with confirmed joint infection during 30 days after operation without the use of non-resorbable implants and up to 1 year after procedures with non-resorbable implants. Demographic and medical data about potential risk factors of SAAA were analyzed: patients age, sex, operated joint, type of procedure, primary or revision procedure, the use of drains, usage of non-absorbable implant, time of surgery, BMI, time lapse from index operation to diagnosis of SAAA, length of hospital stay, causative microorganisms. All operations were performed by eight different but experienced surgeons, in operation theatre with vertical laminar flow with the capacity of 50 air exchanges per hour. The perioperative antibiotic prophylaxis consisted of one preoperative dose 1 g cefazolin in simple arthroscopies, which was prolonged to 24 hours with 3 doses in cases of implantation of non-absorbable implant (55.8% of patients). Results From 794 cases 4 have been infected: 2 after knee arthrolysis, one after ACL reconstruction and one after rotator cuff repair. There were 2 early, with manifestation within 30 days, and 2 late-onset SAAA. Infections occurred in 0.5% of all arthroscopies and in 0.47% of knee arthroscopies alone. Patients age and time of surgery have not been found significantly different in infected and non-infected cases, whereas age and time of surgery have been significantly different in revision and non-revision cases. Conclusions Primarily aseptic arthroscopic procedures performed with respect to actual perioperative preventive measures have a low risk of postoperative septic arthritis. The risk increases with patients age and time of operation, but not significantly.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"76 1","pages":"351 - 357"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69110165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Domżał-Magrowska, Marek K. Kowalski, E. Małecka-Wojciesko
Abstract Introduction Lactose intolerance, due to lactase deficiency, may overlap with the symptoms of irritable bowel syndrome (IBS) or lead to an incorrect diagnosis of IBS. Identification of lactose intolerance and lactase deficiency enables targeted treatment to be implemented. The aim of the study was to search for a frequency of a lactose intolerance symptoms in patients with IBS, depending on the results of hydrogen breath test (HBT). Materials and Methods The study involved 56 patients with IBS and 23 healthy people. Both, IBS patients and healthy controls, were asked to complete a survey about IBS symptoms and tolerance of lactose-containing products. A HBT was performed on all subjects. Results Lactase deficiency was diagnosed in 60.7% of patients with IBS and 43.5% of control group. The differences between the study groups were not statistically significant. No statistically significant differences were observed between the incidence of lactase deficiency depending on the type of IBS. In the group of patients with IBS and normal HBT results, as well as in patients with IBS and lactase deficiency, there were no statistically significant differences in the frequency of lactose intolerance symptoms. However, among people with normal HBT results, patients with IBS reported symptoms of intolerance after consuming lactose-containing foods significantly more often compared to the control group (p<0.05). Conclusions Frequency of lactase deficiency does not differ between IBS patients and healthy individuals. Patients with IBS are more likely to report symptoms of lactose intolerance, regardless of the HBT result, compared to healthy individuals.
{"title":"Subjective, but not documented, lactose intolerance accompanies irritable bowel syndrome","authors":"D. Domżał-Magrowska, Marek K. Kowalski, E. Małecka-Wojciesko","doi":"10.2478/ahem-2022-0027","DOIUrl":"https://doi.org/10.2478/ahem-2022-0027","url":null,"abstract":"Abstract Introduction Lactose intolerance, due to lactase deficiency, may overlap with the symptoms of irritable bowel syndrome (IBS) or lead to an incorrect diagnosis of IBS. Identification of lactose intolerance and lactase deficiency enables targeted treatment to be implemented. The aim of the study was to search for a frequency of a lactose intolerance symptoms in patients with IBS, depending on the results of hydrogen breath test (HBT). Materials and Methods The study involved 56 patients with IBS and 23 healthy people. Both, IBS patients and healthy controls, were asked to complete a survey about IBS symptoms and tolerance of lactose-containing products. A HBT was performed on all subjects. Results Lactase deficiency was diagnosed in 60.7% of patients with IBS and 43.5% of control group. The differences between the study groups were not statistically significant. No statistically significant differences were observed between the incidence of lactase deficiency depending on the type of IBS. In the group of patients with IBS and normal HBT results, as well as in patients with IBS and lactase deficiency, there were no statistically significant differences in the frequency of lactose intolerance symptoms. However, among people with normal HBT results, patients with IBS reported symptoms of intolerance after consuming lactose-containing foods significantly more often compared to the control group (p<0.05). Conclusions Frequency of lactase deficiency does not differ between IBS patients and healthy individuals. Patients with IBS are more likely to report symptoms of lactose intolerance, regardless of the HBT result, compared to healthy individuals.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"76 1","pages":"444 - 449"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69110208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The World Health Organization reports that the prevalent problem of excessive weight and obesity currently affects about 1.9 billion people worldwide and is the fifth most common death factor among patients. In view of the growing number of patients with obesity, attention is drawn to the insufficient effectiveness of behavioral treatment methods. In addition to genetic and environmental factors leading to the consumption of excess energy in the diet and the accumulation of adipose tissue, attention is paid to the role of intestinal microbiota in maintaining a normal body weight. Dysbiosis – a disorder in the composition of the gut microbiota – is mentioned as one of the contributing factors to the development of metabolic diseases, including obesity, type 2 diabetes, and cardiovascular disorders. The human gastrointestinal tract is colonized largely by a group of Gram-negative bacteria that are indicated to be a source of lipopolysaccharide (LPS), associated with inducing systemic inflammation and endotoxemia. Research suggests that disturbances in the gut microbiota, leading to damage to the intestinal barrier and an increase in circulating LPS, are implicated in obesity and other metabolic disorders. Plasma LPS and lipopolysaccharide-binding protein (LBP) levels have been shown to be elevated in individuals with excess body weight. Bariatric surgery has become a popular treatment option, leading to stable weight loss and an improvement in obesity-related conditions. The aim of this study was to characterize the factors that promote the induction of metabolic endotoxemia and its associated health consequences, along with the presentation of their changes after bariatric surgery.
{"title":"Intestinal barrier disorders and metabolic endotoxemia in obesity: Current knowledge","authors":"M. Potrykus, M. Szymański, Ł. Kaska, A. Janczy","doi":"10.2478/ahem-2022-0008","DOIUrl":"https://doi.org/10.2478/ahem-2022-0008","url":null,"abstract":"Abstract The World Health Organization reports that the prevalent problem of excessive weight and obesity currently affects about 1.9 billion people worldwide and is the fifth most common death factor among patients. In view of the growing number of patients with obesity, attention is drawn to the insufficient effectiveness of behavioral treatment methods. In addition to genetic and environmental factors leading to the consumption of excess energy in the diet and the accumulation of adipose tissue, attention is paid to the role of intestinal microbiota in maintaining a normal body weight. Dysbiosis – a disorder in the composition of the gut microbiota – is mentioned as one of the contributing factors to the development of metabolic diseases, including obesity, type 2 diabetes, and cardiovascular disorders. The human gastrointestinal tract is colonized largely by a group of Gram-negative bacteria that are indicated to be a source of lipopolysaccharide (LPS), associated with inducing systemic inflammation and endotoxemia. Research suggests that disturbances in the gut microbiota, leading to damage to the intestinal barrier and an increase in circulating LPS, are implicated in obesity and other metabolic disorders. Plasma LPS and lipopolysaccharide-binding protein (LBP) levels have been shown to be elevated in individuals with excess body weight. Bariatric surgery has become a popular treatment option, leading to stable weight loss and an improvement in obesity-related conditions. The aim of this study was to characterize the factors that promote the induction of metabolic endotoxemia and its associated health consequences, along with the presentation of their changes after bariatric surgery.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"76 1","pages":"71 - 80"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69110288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Şükrü Ateş, H. Ülger, S. Yılmaz, G. Karatoprak, Ö. Al, Sümeyye Uçar, M. Taştan, A. Tokpınar, Ş. Alpa, A. Farooqi
Abstract Introduction Mistletoe has been used alone or as a complementary therapy in the treatment of different diseases for years. In this study, the antitumoral effect of mistletoe fruit extract on Ehrlich ascites tumor (EAT) cells was evaluated. Materials and Methods EAT cells from preformed stock mice were transferred to culture dishes containing 5-fluorouracil (5-FU) and mistletoe extracts at different doses (100, 200, 400, and 800 μg/ml). These cells were incubated at 37 °C in an environment with 95% humidity and 5% CO2. At the end of the incubations, the apoptosis status of the cells, cell cycle, mitochondrial membrane potential, and proliferation status with the argyrophilic (Ag) nucleolar organizer region staining (NORs) method were evaluated. Results As a result, it was observed that the mistletoe fruit extract and 5-FU induce apoptosis of EAT cells. It was concluded that the 5-FU substance arrests the cell cycle at the G0/G1 stage, while the mistletoe arrests the cell cycle at the S and G2/M stages. The depolarization rate of the mistletoe treated cells was higher. As a result of the evaluation made with the AgNORs method, it was seen that mistletoe and 5-FU could be effective in reducing the proliferation of EAT cells. Conclusions It was seen that mistletoe fruit extract could be effective in stimulating the apoptosis and depolarization of cancer cells. The results of other studies in the literature and our study support each other. It was concluded that the mistletoe plant may be useful in cancer treatment.
{"title":"Evaluation of antitumoral effect of mistletoe fruit extract on Ehrlich ascites tumor cells with muse cell analyzer and argyrophilic nucleolar organizer region staining method","authors":"Şükrü Ateş, H. Ülger, S. Yılmaz, G. Karatoprak, Ö. Al, Sümeyye Uçar, M. Taştan, A. Tokpınar, Ş. Alpa, A. Farooqi","doi":"10.2478/ahem-2022-0014","DOIUrl":"https://doi.org/10.2478/ahem-2022-0014","url":null,"abstract":"Abstract Introduction Mistletoe has been used alone or as a complementary therapy in the treatment of different diseases for years. In this study, the antitumoral effect of mistletoe fruit extract on Ehrlich ascites tumor (EAT) cells was evaluated. Materials and Methods EAT cells from preformed stock mice were transferred to culture dishes containing 5-fluorouracil (5-FU) and mistletoe extracts at different doses (100, 200, 400, and 800 μg/ml). These cells were incubated at 37 °C in an environment with 95% humidity and 5% CO2. At the end of the incubations, the apoptosis status of the cells, cell cycle, mitochondrial membrane potential, and proliferation status with the argyrophilic (Ag) nucleolar organizer region staining (NORs) method were evaluated. Results As a result, it was observed that the mistletoe fruit extract and 5-FU induce apoptosis of EAT cells. It was concluded that the 5-FU substance arrests the cell cycle at the G0/G1 stage, while the mistletoe arrests the cell cycle at the S and G2/M stages. The depolarization rate of the mistletoe treated cells was higher. As a result of the evaluation made with the AgNORs method, it was seen that mistletoe and 5-FU could be effective in reducing the proliferation of EAT cells. Conclusions It was seen that mistletoe fruit extract could be effective in stimulating the apoptosis and depolarization of cancer cells. The results of other studies in the literature and our study support each other. It was concluded that the mistletoe plant may be useful in cancer treatment.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"76 1","pages":"209 - 219"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69110349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Introduction Within 5 years, the number of pregnant smokers in Poland decreased by 5%. Still, 6% of pregnant women are active and 18% are passive smokers, and 5% smoke and drink alcohol. The study examined the levels of cotinine concentration in the blood of pregnant women and in the umbilical cord blood of their children in relation to the number of cigarettes smoked; the places and persons conducive to exposure of pregnant women to tobacco smoke were determined. Materials and Methods The study included 123 women who have entered medical facilities for childbirth during physiological pregnancy, who filled out a questionnaire about their lifestyle. Moreover, venous and umbilical blood was collected from them and their newborns for cotinine evaluation. The nicotine marker was determined by the UPLC/MS/MS analytical method. Results When examining the frequency of smoking and exposure to smoke, it was found that 38% of the respondents concealed their smoking status by giving false answers, as shown by the biochemical analysis of the presence of cotinine in the blood. A significant correlation was found between the cotinine levels of exposed and smoking mothers and their babies. Pregnant smokers were in the presence of smokers more often (p = 0.019). Moreover, it was found that the presence of women in the company of smokers who smoked more than 20 cigarettes/day resulted in a significant (p = 0.011) increase of cotinine in the umbilical blood in 31.5% of newborns, higher than the concentration in mothers. Conclusions Protecting women from passive smoking requires extensive educational measures. The inclusion of cotinine level assessment in pregnant women in the program of the standard of perinatal examinations should be considered in order to protect children from diseases resulting from the effects of nicotine in the embryonic period.
{"title":"Cotinine as an indicator of fetal exposure to active and passive smoking in pregnant women","authors":"Marta Dulęba, B. Kozakiewicz","doi":"10.2478/ahem-2022-0037","DOIUrl":"https://doi.org/10.2478/ahem-2022-0037","url":null,"abstract":"Abstract Introduction Within 5 years, the number of pregnant smokers in Poland decreased by 5%. Still, 6% of pregnant women are active and 18% are passive smokers, and 5% smoke and drink alcohol. The study examined the levels of cotinine concentration in the blood of pregnant women and in the umbilical cord blood of their children in relation to the number of cigarettes smoked; the places and persons conducive to exposure of pregnant women to tobacco smoke were determined. Materials and Methods The study included 123 women who have entered medical facilities for childbirth during physiological pregnancy, who filled out a questionnaire about their lifestyle. Moreover, venous and umbilical blood was collected from them and their newborns for cotinine evaluation. The nicotine marker was determined by the UPLC/MS/MS analytical method. Results When examining the frequency of smoking and exposure to smoke, it was found that 38% of the respondents concealed their smoking status by giving false answers, as shown by the biochemical analysis of the presence of cotinine in the blood. A significant correlation was found between the cotinine levels of exposed and smoking mothers and their babies. Pregnant smokers were in the presence of smokers more often (p = 0.019). Moreover, it was found that the presence of women in the company of smokers who smoked more than 20 cigarettes/day resulted in a significant (p = 0.011) increase of cotinine in the umbilical blood in 31.5% of newborns, higher than the concentration in mothers. Conclusions Protecting women from passive smoking requires extensive educational measures. The inclusion of cotinine level assessment in pregnant women in the program of the standard of perinatal examinations should be considered in order to protect children from diseases resulting from the effects of nicotine in the embryonic period.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"76 1","pages":"358 - 368"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69110784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Malinowska, Alicja Nowak-Zduńczyk, Anna Merecz-Sadowska, A. Szczepańska, D. Kaczmarczyk, Katarzyna Bliźniewska-Kowalska, H. Zielińska-Bliźniewska
Abstract Background Cancers of the head and neck can damage the brain, spinal cord, and nerves, as well as the sense organs responsible for contact with the outside world. Oncogenic transformation occurs following mutations that change the function of specific genes, such tumor suppressor genes or oncogenes, and their encoded protein products. Therefore, the aim of this study was to evaluate the relationship between the occurrence of MTHFD1 c.1958G>A and TCN2 c.776G>C gene polymorphisms and the risk of oral cavity cancer. Materials and methods The study population consisted of 439 patients and 200 healthy subjects. Genomic DNA was extracted from peripheral blood and from paraffin-embedded tissue. Analysis of the gene polymorphisms was performed using polymerase chain reaction–restriction fragment length polymorphism. Results Statistically significant differences were found in the distribution of genotypes of the rs2236225 and rs1801198 polymorphisms between patients and controls. Regarding MTHFD1 c.1958G>A, the GA genotype (p<0.0001, OR = 2.91, 95% CI = 1.88–4.49-GA) was more common among patients than healthy subjects. Regarding TCN2 c.776G>C, the frequency of CC genotype (p< 0.0001, OR = 0.17, 95% CI = 0.10–0.33-CC) was significantly less common among patients than healthy subjects. Tobacco smoking, alcohol use, and male gender (p<0.0001) were found to be predictors of the disease. Conclusion The results suggest that the MTHFD1 c.1958G>A polymorphism may be associated with a greater risk of oral cavity cancer, whereas a protective effect has been found for the TCN2 c.776G>C polymorphism.
头颈部癌症可损害大脑、脊髓和神经，以及负责与外界接触的感觉器官。致癌转化发生在改变特定基因(如肿瘤抑制基因或致癌基因)及其编码蛋白产物功能的突变之后。因此，本研究的目的是评估MTHFD1 C . 1958g >A和TCN2 C . 776g >C基因多态性的发生与口腔癌风险的关系。材料与方法研究人群为439例患者和200例健康受试者。从外周血和石蜡包埋组织中提取基因组DNA。基因多态性分析采用聚合酶链反应-限制性片段长度多态性。结果rs2236225和rs1801198基因型多态性在患者与对照组间的分布差异有统计学意义。对于MTHFD1 c.1958G>A, GA基因型(pC, CC基因型频率(p< 0.0001, OR = 0.17, 95% CI = 0.10 - 0.33 CC)在患者中的发生率明显低于健康者。吸烟、饮酒和男性性别(pA多态性)可能与口腔癌风险增加有关，而TCN2 C . 776g >C多态性则具有保护作用。
{"title":"MTHFD1 c.1958G>A and TCN2 c.776G>C polymorphisms of folate metabolism genes and their implication for oral cavity cancer","authors":"K. Malinowska, Alicja Nowak-Zduńczyk, Anna Merecz-Sadowska, A. Szczepańska, D. Kaczmarczyk, Katarzyna Bliźniewska-Kowalska, H. Zielińska-Bliźniewska","doi":"10.2478/ahem-2021-0041","DOIUrl":"https://doi.org/10.2478/ahem-2021-0041","url":null,"abstract":"Abstract Background Cancers of the head and neck can damage the brain, spinal cord, and nerves, as well as the sense organs responsible for contact with the outside world. Oncogenic transformation occurs following mutations that change the function of specific genes, such tumor suppressor genes or oncogenes, and their encoded protein products. Therefore, the aim of this study was to evaluate the relationship between the occurrence of MTHFD1 c.1958G>A and TCN2 c.776G>C gene polymorphisms and the risk of oral cavity cancer. Materials and methods The study population consisted of 439 patients and 200 healthy subjects. Genomic DNA was extracted from peripheral blood and from paraffin-embedded tissue. Analysis of the gene polymorphisms was performed using polymerase chain reaction–restriction fragment length polymorphism. Results Statistically significant differences were found in the distribution of genotypes of the rs2236225 and rs1801198 polymorphisms between patients and controls. Regarding MTHFD1 c.1958G>A, the GA genotype (p<0.0001, OR = 2.91, 95% CI = 1.88–4.49-GA) was more common among patients than healthy subjects. Regarding TCN2 c.776G>C, the frequency of CC genotype (p< 0.0001, OR = 0.17, 95% CI = 0.10–0.33-CC) was significantly less common among patients than healthy subjects. Tobacco smoking, alcohol use, and male gender (p<0.0001) were found to be predictors of the disease. Conclusion The results suggest that the MTHFD1 c.1958G>A polymorphism may be associated with a greater risk of oral cavity cancer, whereas a protective effect has been found for the TCN2 c.776G>C polymorphism.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"76 1","pages":"30 - 38"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69109803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agnieszka Owczarczyk-Saczonek, Marta Kasprowicz-Furmańczyk, M. Krajewska-Włodarczyk, W. Placek
Abstract Psoriasis is one of the most common chronic inflammatory skin diseases, constituting a significant health and socioeconomic problem. Despite numerous therapeutic options, the results of treatment very often remain insufficient. It is extremely important to remember that many external factors impact the effectiveness of therapy. This article discusses the importance of emollients in therapy and the influence of infectious agents and injuries on the course of psoriasis. Understanding the above-mentioned factors in the treatment of psoriasis is critical to achieve satisfactory therapeutic effects.
{"title":"The impact of external factors on psoriasis","authors":"Agnieszka Owczarczyk-Saczonek, Marta Kasprowicz-Furmańczyk, M. Krajewska-Włodarczyk, W. Placek","doi":"10.2478/ahem-2022-0017","DOIUrl":"https://doi.org/10.2478/ahem-2022-0017","url":null,"abstract":"Abstract Psoriasis is one of the most common chronic inflammatory skin diseases, constituting a significant health and socioeconomic problem. Despite numerous therapeutic options, the results of treatment very often remain insufficient. It is extremely important to remember that many external factors impact the effectiveness of therapy. This article discusses the importance of emollients in therapy and the influence of infectious agents and injuries on the course of psoriasis. Understanding the above-mentioned factors in the treatment of psoriasis is critical to achieve satisfactory therapeutic effects.","PeriodicalId":20347,"journal":{"name":"Postȩpy higieny i medycyny doświadczalnej","volume":"76 1","pages":"177 - 187"},"PeriodicalIF":0.3,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69109923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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