The experiment aimed to induction of insensitive peanut somatic embryo (SE) to culture filtrate medium from various races of S. rolfsii and its plantlet regeneration. To achieve these objective, the experiments have been done in several steps: somatic embryo development, preparation of culture filtrate (CF) medium from various races of S. rolfsii, induction of insensitive peanut somatic embryo and peanut plantlet regeneration of insensitive somatic embryo. Result of experiment showed that selected SEs on selective medium containing culture filtrate of S. rolfsii one race or two races or three races or four races were specifically insensitive only on selective medium containing culture filtrate one race or two or three or four races of S. rolfsii. All SEs selected in culture filtrate medium of four races S. rolfsii (race 1, 6, 8, and 9) were more insensitive on culture filtrate medium of S. rolfsii races compared with SEs that only selected in culture filtrate medium of one race. Selected insensitive SEs had ability to form normal seedling (50.5-65.5%) and abnormal germinated SEs (23.5 - 30.5%). Normal germinated SEs developed to form plantlets. These plantlets were developed to produced peanut plant R1 generation and peanut seeds. Further evaluation using subsequent generation of peanut (R1 and R2) plants need to be conducted to testing peanut line resistance to stem rot disease (S. rolfsii) in the field.The experiment aimed to induction of insensitive peanut somatic embryo (SE) to culture filtrate medium from various races of S. rolfsii and its plantlet regeneration. To achieve these objective, the experiments have been done in several steps: somatic embryo development, preparation of culture filtrate (CF) medium from various races of S. rolfsii, induction of insensitive peanut somatic embryo and peanut plantlet regeneration of insensitive somatic embryo. Result of experiment showed that selected SEs on selective medium containing culture filtrate of S. rolfsii one race or two races or three races or four races were specifically insensitive only on selective medium containing culture filtrate one race or two or three or four races of S. rolfsii. All SEs selected in culture filtrate medium of four races S. rolfsii (race 1, 6, 8, and 9) were more insensitive on culture filtrate medium of S. rolfsii races compared with SEs that only selected in culture filtrate medium of one race. Selected insensitive SEs h...
{"title":"Induction of insensitive peanut somatic embryo to medium containing culture filtrate from various races of Sclerotium rolfsii and plantlet regeneration","authors":"A. Hemon, Sumarjan, A. Hanafi","doi":"10.1063/1.5141292","DOIUrl":"https://doi.org/10.1063/1.5141292","url":null,"abstract":"The experiment aimed to induction of insensitive peanut somatic embryo (SE) to culture filtrate medium from various races of S. rolfsii and its plantlet regeneration. To achieve these objective, the experiments have been done in several steps: somatic embryo development, preparation of culture filtrate (CF) medium from various races of S. rolfsii, induction of insensitive peanut somatic embryo and peanut plantlet regeneration of insensitive somatic embryo. Result of experiment showed that selected SEs on selective medium containing culture filtrate of S. rolfsii one race or two races or three races or four races were specifically insensitive only on selective medium containing culture filtrate one race or two or three or four races of S. rolfsii. All SEs selected in culture filtrate medium of four races S. rolfsii (race 1, 6, 8, and 9) were more insensitive on culture filtrate medium of S. rolfsii races compared with SEs that only selected in culture filtrate medium of one race. Selected insensitive SEs had ability to form normal seedling (50.5-65.5%) and abnormal germinated SEs (23.5 - 30.5%). Normal germinated SEs developed to form plantlets. These plantlets were developed to produced peanut plant R1 generation and peanut seeds. Further evaluation using subsequent generation of peanut (R1 and R2) plants need to be conducted to testing peanut line resistance to stem rot disease (S. rolfsii) in the field.The experiment aimed to induction of insensitive peanut somatic embryo (SE) to culture filtrate medium from various races of S. rolfsii and its plantlet regeneration. To achieve these objective, the experiments have been done in several steps: somatic embryo development, preparation of culture filtrate (CF) medium from various races of S. rolfsii, induction of insensitive peanut somatic embryo and peanut plantlet regeneration of insensitive somatic embryo. Result of experiment showed that selected SEs on selective medium containing culture filtrate of S. rolfsii one race or two races or three races or four races were specifically insensitive only on selective medium containing culture filtrate one race or two or three or four races of S. rolfsii. All SEs selected in culture filtrate medium of four races S. rolfsii (race 1, 6, 8, and 9) were more insensitive on culture filtrate medium of S. rolfsii races compared with SEs that only selected in culture filtrate medium of one race. Selected insensitive SEs h...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80533059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Syahrul, A. Cahyono, M. Mirmanto, H. S. Tira, Sukmawaty, G. Putra
Drying is one way of post-harvest handling to extend the shelf life, especially for agricultural crops. In general, post-harvest drying is only done naturally by using heat from solar energy or commonly called conventional drying. In this study, the grain was dried using a fluidized bed dryer. However, the fluidized bed dryer was modified by adding a pipe heat exchanger. The purpose of this study was to find the effect of grain mass and air intake temperatures on drying time. The Indonesian government has carried out SNI provisions for milling the maximum water content in the material which is 14%. In this study, the grain used was the newly harvested grain where the water content was about 20% with a tolerance of ± 1%. The mass variations in this study were 0.4 kg, 0.6 kg and 0.8 kg and the temperature variations applied were 55°C, 60°C, and 65° C. The results showed the highest heat loss occurs at the highest mass because the air velocity is not able to circulate properly.Drying is one way of post-harvest handling to extend the shelf life, especially for agricultural crops. In general, post-harvest drying is only done naturally by using heat from solar energy or commonly called conventional drying. In this study, the grain was dried using a fluidized bed dryer. However, the fluidized bed dryer was modified by adding a pipe heat exchanger. The purpose of this study was to find the effect of grain mass and air intake temperatures on drying time. The Indonesian government has carried out SNI provisions for milling the maximum water content in the material which is 14%. In this study, the grain used was the newly harvested grain where the water content was about 20% with a tolerance of ± 1%. The mass variations in this study were 0.4 kg, 0.6 kg and 0.8 kg and the temperature variations applied were 55°C, 60°C, and 65° C. The results showed the highest heat loss occurs at the highest mass because the air velocity is not able to circulate properly.
{"title":"The effect of grain mass variations on drying time by adding a pipe heat exchanger to a fluidized bed dryer","authors":"Syahrul, A. Cahyono, M. Mirmanto, H. S. Tira, Sukmawaty, G. Putra","doi":"10.1063/1.5141298","DOIUrl":"https://doi.org/10.1063/1.5141298","url":null,"abstract":"Drying is one way of post-harvest handling to extend the shelf life, especially for agricultural crops. In general, post-harvest drying is only done naturally by using heat from solar energy or commonly called conventional drying. In this study, the grain was dried using a fluidized bed dryer. However, the fluidized bed dryer was modified by adding a pipe heat exchanger. The purpose of this study was to find the effect of grain mass and air intake temperatures on drying time. The Indonesian government has carried out SNI provisions for milling the maximum water content in the material which is 14%. In this study, the grain used was the newly harvested grain where the water content was about 20% with a tolerance of ± 1%. The mass variations in this study were 0.4 kg, 0.6 kg and 0.8 kg and the temperature variations applied were 55°C, 60°C, and 65° C. The results showed the highest heat loss occurs at the highest mass because the air velocity is not able to circulate properly.Drying is one way of post-harvest handling to extend the shelf life, especially for agricultural crops. In general, post-harvest drying is only done naturally by using heat from solar energy or commonly called conventional drying. In this study, the grain was dried using a fluidized bed dryer. However, the fluidized bed dryer was modified by adding a pipe heat exchanger. The purpose of this study was to find the effect of grain mass and air intake temperatures on drying time. The Indonesian government has carried out SNI provisions for milling the maximum water content in the material which is 14%. In this study, the grain used was the newly harvested grain where the water content was about 20% with a tolerance of ± 1%. The mass variations in this study were 0.4 kg, 0.6 kg and 0.8 kg and the temperature variations applied were 55°C, 60°C, and 65° C. The results showed the highest heat loss occurs at the highest mass because the air velocity is not able to circulate properly.","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89457020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preface: XVII. International Scientific Conference: Dynamics of Rigid and Deformable Bodies 2019","authors":"SkocilasovaBlanka, LenhardRichard, SoukupJosef","doi":"10.1063/1.5140860","DOIUrl":"https://doi.org/10.1063/1.5140860","url":null,"abstract":"","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88542075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Prasedya, Brigitta A. F. D. Geraldine, Nurul Noviandi Nahdia Putri, A. S. Abidin, A. Jupri, H. Sunarpi
The excessive use of ingonaic fertililizer in agriculture system production reduces soil fertility and polluted out environment. Therefore, the use of organic ferlizer is increased recently. Many authors report that macroalgae is an important source of organic biostimulant and biofertilizer. This is due to the fact that liquid extract contains phytohormone, and solid extract contains essential elements, stimulating growth and production of plants. This article reports effect of solid and liquid extracts on growth and yield of rice plants. Liquid extract (10%) was sprayed during vegetative growth of the plants. On the other hand, liquid extract (5%) was applied directly to growth media. Then, the growth parameters (plant height, tiller number, shoot dry weight and root dry weight), and yield parameters (penicle number and grain weight), were measured during harvesting time. The results show that solid and liquid extracts of Sargassum crassifolium increased the growth and yield of rice plants. This indicates that solid extract of Sargassum crassifolium could be developed as a source of organic fertilizer. On the other hand, liquid extract of Sargassum crassiflium could also be used as a source of organic biostimulant. Both products should support suitable agriculture production.The excessive use of ingonaic fertililizer in agriculture system production reduces soil fertility and polluted out environment. Therefore, the use of organic ferlizer is increased recently. Many authors report that macroalgae is an important source of organic biostimulant and biofertilizer. This is due to the fact that liquid extract contains phytohormone, and solid extract contains essential elements, stimulating growth and production of plants. This article reports effect of solid and liquid extracts on growth and yield of rice plants. Liquid extract (10%) was sprayed during vegetative growth of the plants. On the other hand, liquid extract (5%) was applied directly to growth media. Then, the growth parameters (plant height, tiller number, shoot dry weight and root dry weight), and yield parameters (penicle number and grain weight), were measured during harvesting time. The results show that solid and liquid extracts of Sargassum crassifolium increased the growth and yield of rice plants. This indicate...
{"title":"Effect of solid and liquid extract of Sargassum crassifolium on growth and yield of rice plant","authors":"E. Prasedya, Brigitta A. F. D. Geraldine, Nurul Noviandi Nahdia Putri, A. S. Abidin, A. Jupri, H. Sunarpi","doi":"10.1063/1.5141321","DOIUrl":"https://doi.org/10.1063/1.5141321","url":null,"abstract":"The excessive use of ingonaic fertililizer in agriculture system production reduces soil fertility and polluted out environment. Therefore, the use of organic ferlizer is increased recently. Many authors report that macroalgae is an important source of organic biostimulant and biofertilizer. This is due to the fact that liquid extract contains phytohormone, and solid extract contains essential elements, stimulating growth and production of plants. This article reports effect of solid and liquid extracts on growth and yield of rice plants. Liquid extract (10%) was sprayed during vegetative growth of the plants. On the other hand, liquid extract (5%) was applied directly to growth media. Then, the growth parameters (plant height, tiller number, shoot dry weight and root dry weight), and yield parameters (penicle number and grain weight), were measured during harvesting time. The results show that solid and liquid extracts of Sargassum crassifolium increased the growth and yield of rice plants. This indicates that solid extract of Sargassum crassifolium could be developed as a source of organic fertilizer. On the other hand, liquid extract of Sargassum crassiflium could also be used as a source of organic biostimulant. Both products should support suitable agriculture production.The excessive use of ingonaic fertililizer in agriculture system production reduces soil fertility and polluted out environment. Therefore, the use of organic ferlizer is increased recently. Many authors report that macroalgae is an important source of organic biostimulant and biofertilizer. This is due to the fact that liquid extract contains phytohormone, and solid extract contains essential elements, stimulating growth and production of plants. This article reports effect of solid and liquid extracts on growth and yield of rice plants. Liquid extract (10%) was sprayed during vegetative growth of the plants. On the other hand, liquid extract (5%) was applied directly to growth media. Then, the growth parameters (plant height, tiller number, shoot dry weight and root dry weight), and yield parameters (penicle number and grain weight), were measured during harvesting time. The results show that solid and liquid extracts of Sargassum crassifolium increased the growth and yield of rice plants. This indicate...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77380491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Frediansyah, M. Kurniadi, Nurul Noviandi Nahdia Putri, E. Prasedya
Our previous finding showed that the single culture of Lactobacillus plantarum has a maximum time of 18 hours in order to produce carboxyl cellulose and amylase during cassava fermentation. These two enzymes are important in order to give a final characteristic of modified cassava flour including texture, aroma, flavor and taste. The cyanide content of cassava tuber also decreases during its fermentation process. We therefore aimed to modify the starter using co-culture starter of L. plantarum and L. acidophilusand to analyze the production of extracellular amylase and carboxymethyl cellulose during cassava fermentation. The maximum of carboxymethyl cellulose and amylase activity of 13.76 × 10−3 U/ml and 8.31 × 10−3 U/ml, respectively, were obtained from filtrate that has been incubated at 37°C for 23 and 26 h, respectively under static condition. The Km of CMCase and amylase by co-culture starterof L. plantarum and L. acidophilus (1:1) were 0.4791 × 10−3 U/ml and 0.6133 × 10−3 U/ml, respectively. Vmax for CMCase and amylase were 0.8902 × 10−3 and 0.8703 × 10−3 g/mL, respectivelyOur previous finding showed that the single culture of Lactobacillus plantarum has a maximum time of 18 hours in order to produce carboxyl cellulose and amylase during cassava fermentation. These two enzymes are important in order to give a final characteristic of modified cassava flour including texture, aroma, flavor and taste. The cyanide content of cassava tuber also decreases during its fermentation process. We therefore aimed to modify the starter using co-culture starter of L. plantarum and L. acidophilusand to analyze the production of extracellular amylase and carboxymethyl cellulose during cassava fermentation. The maximum of carboxymethyl cellulose and amylase activity of 13.76 × 10−3 U/ml and 8.31 × 10−3 U/ml, respectively, were obtained from filtrate that has been incubated at 37°C for 23 and 26 h, respectively under static condition. The Km of CMCase and amylase by co-culture starterof L. plantarum and L. acidophilus (1:1) were 0.4791 × 10−3 U/ml and 0.6133 × 10−3 U/ml, respectively. Vmax fo...
{"title":"The kinetics of enzymes that involved in cassava fermentation produce by co-culture starter of two lactic acid bacteria","authors":"A. Frediansyah, M. Kurniadi, Nurul Noviandi Nahdia Putri, E. Prasedya","doi":"10.1063/1.5141280","DOIUrl":"https://doi.org/10.1063/1.5141280","url":null,"abstract":"Our previous finding showed that the single culture of Lactobacillus plantarum has a maximum time of 18 hours in order to produce carboxyl cellulose and amylase during cassava fermentation. These two enzymes are important in order to give a final characteristic of modified cassava flour including texture, aroma, flavor and taste. The cyanide content of cassava tuber also decreases during its fermentation process. We therefore aimed to modify the starter using co-culture starter of L. plantarum and L. acidophilusand to analyze the production of extracellular amylase and carboxymethyl cellulose during cassava fermentation. The maximum of carboxymethyl cellulose and amylase activity of 13.76 × 10−3 U/ml and 8.31 × 10−3 U/ml, respectively, were obtained from filtrate that has been incubated at 37°C for 23 and 26 h, respectively under static condition. The Km of CMCase and amylase by co-culture starterof L. plantarum and L. acidophilus (1:1) were 0.4791 × 10−3 U/ml and 0.6133 × 10−3 U/ml, respectively. Vmax for CMCase and amylase were 0.8902 × 10−3 and 0.8703 × 10−3 g/mL, respectivelyOur previous finding showed that the single culture of Lactobacillus plantarum has a maximum time of 18 hours in order to produce carboxyl cellulose and amylase during cassava fermentation. These two enzymes are important in order to give a final characteristic of modified cassava flour including texture, aroma, flavor and taste. The cyanide content of cassava tuber also decreases during its fermentation process. We therefore aimed to modify the starter using co-culture starter of L. plantarum and L. acidophilusand to analyze the production of extracellular amylase and carboxymethyl cellulose during cassava fermentation. The maximum of carboxymethyl cellulose and amylase activity of 13.76 × 10−3 U/ml and 8.31 × 10−3 U/ml, respectively, were obtained from filtrate that has been incubated at 37°C for 23 and 26 h, respectively under static condition. The Km of CMCase and amylase by co-culture starterof L. plantarum and L. acidophilus (1:1) were 0.4791 × 10−3 U/ml and 0.6133 × 10−3 U/ml, respectively. Vmax fo...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"134 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77387999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Novita Tri Artiningrum, Dara Puspita Anggraini, Y. Zamroni
Four species of Cristatogobius were found in the mangrove ecosystem during diversity of Indonesian gobies survey, they are C. aurimaculatus, C. lophius, C. nonatoae, and C. rubripectoralis. In the previous study, only two species of this genus ware reported in Indonesia: C. lophius from Kakaban and Sangalaki islands near Kalimantan and C. rubripectoralis from southern Java (only photographs). We reported more species of Cristatogobiusin Indonesia and extended the distribution of these species especially in Java, Sulawesi and Lesser Sunda Islands.
{"title":"The record of Cristatogobius (Teleostei: Gobiidae) in Indonesia","authors":"Novita Tri Artiningrum, Dara Puspita Anggraini, Y. Zamroni","doi":"10.1063/1.5141303","DOIUrl":"https://doi.org/10.1063/1.5141303","url":null,"abstract":"Four species of Cristatogobius were found in the mangrove ecosystem during diversity of Indonesian gobies survey, they are C. aurimaculatus, C. lophius, C. nonatoae, and C. rubripectoralis. In the previous study, only two species of this genus ware reported in Indonesia: C. lophius from Kakaban and Sangalaki islands near Kalimantan and C. rubripectoralis from southern Java (only photographs). We reported more species of Cristatogobiusin Indonesia and extended the distribution of these species especially in Java, Sulawesi and Lesser Sunda Islands.","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90370620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to determine the effect of expired bread supplementation on sheep rations in digestibility and rumen fermentability in vitro. The study used a Compeletely Randomized Design (CRD) with 4 treatments and 3 replications. The first treatment P0 was a control, sheep fed with 100% basal concentrates. The second treatment P1, sheep fed with 95% basal concentrates plus 5% expired bread. The third treatment P2, sheep fed with 90% basal concentrates plus 10% expired bread. And the last treatment P3, sheep fed with 85% basal concentrates plus 15% expired bread. Data collected were rumen pH, concentration of NH3 (mM), digestibility of dry matter and organic matter (%). The result showed that feed treatments didn’t significantly affect (P>0.05) on rumen pH, concentration of NH3, digestibility of dry matter and organic matter. The average of rumen PH of treatment P0, P1, P2 and P3 respectively was 6.84; 6.86; 6.82 and 6.87. The average of concentration of NH3 of treatment P0, P1, P2 and P3 respectively was 16.17; 16.07; 13.52 and 16.62 mM. The average of dry matter digestibility of treatment P0, P1, P2 and P3 respectively was 51.76; 53.01; 52.16 and 54.71%. The average of organic matter digestibility of treatment P0, P1, P2 and P3 respectively was 53.16; 55.82; 52.24 and 57.59%. It was concluded that expired bread supplementation at the level of 15% can be used to replace basal concentrates in feed male local sheep.This study aims to determine the effect of expired bread supplementation on sheep rations in digestibility and rumen fermentability in vitro. The study used a Compeletely Randomized Design (CRD) with 4 treatments and 3 replications. The first treatment P0 was a control, sheep fed with 100% basal concentrates. The second treatment P1, sheep fed with 95% basal concentrates plus 5% expired bread. The third treatment P2, sheep fed with 90% basal concentrates plus 10% expired bread. And the last treatment P3, sheep fed with 85% basal concentrates plus 15% expired bread. Data collected were rumen pH, concentration of NH3 (mM), digestibility of dry matter and organic matter (%). The result showed that feed treatments didn’t significantly affect (P>0.05) on rumen pH, concentration of NH3, digestibility of dry matter and organic matter. The average of rumen PH of treatment P0, P1, P2 and P3 respectively was 6.84; 6.86; 6.82 and 6.87. The average of concentration of NH3 of treatment P0, P1, P2 and P3 respectively w...
{"title":"The effect of expired bread supplementation in local sheep rations on rumen fermentability and digestibility value in vitro","authors":"A. Hanifa, S. D. Widyawati","doi":"10.1063/1.5141315","DOIUrl":"https://doi.org/10.1063/1.5141315","url":null,"abstract":"This study aims to determine the effect of expired bread supplementation on sheep rations in digestibility and rumen fermentability in vitro. The study used a Compeletely Randomized Design (CRD) with 4 treatments and 3 replications. The first treatment P0 was a control, sheep fed with 100% basal concentrates. The second treatment P1, sheep fed with 95% basal concentrates plus 5% expired bread. The third treatment P2, sheep fed with 90% basal concentrates plus 10% expired bread. And the last treatment P3, sheep fed with 85% basal concentrates plus 15% expired bread. Data collected were rumen pH, concentration of NH3 (mM), digestibility of dry matter and organic matter (%). The result showed that feed treatments didn’t significantly affect (P>0.05) on rumen pH, concentration of NH3, digestibility of dry matter and organic matter. The average of rumen PH of treatment P0, P1, P2 and P3 respectively was 6.84; 6.86; 6.82 and 6.87. The average of concentration of NH3 of treatment P0, P1, P2 and P3 respectively was 16.17; 16.07; 13.52 and 16.62 mM. The average of dry matter digestibility of treatment P0, P1, P2 and P3 respectively was 51.76; 53.01; 52.16 and 54.71%. The average of organic matter digestibility of treatment P0, P1, P2 and P3 respectively was 53.16; 55.82; 52.24 and 57.59%. It was concluded that expired bread supplementation at the level of 15% can be used to replace basal concentrates in feed male local sheep.This study aims to determine the effect of expired bread supplementation on sheep rations in digestibility and rumen fermentability in vitro. The study used a Compeletely Randomized Design (CRD) with 4 treatments and 3 replications. The first treatment P0 was a control, sheep fed with 100% basal concentrates. The second treatment P1, sheep fed with 95% basal concentrates plus 5% expired bread. The third treatment P2, sheep fed with 90% basal concentrates plus 10% expired bread. And the last treatment P3, sheep fed with 85% basal concentrates plus 15% expired bread. Data collected were rumen pH, concentration of NH3 (mM), digestibility of dry matter and organic matter (%). The result showed that feed treatments didn’t significantly affect (P>0.05) on rumen pH, concentration of NH3, digestibility of dry matter and organic matter. The average of rumen PH of treatment P0, P1, P2 and P3 respectively was 6.84; 6.86; 6.82 and 6.87. The average of concentration of NH3 of treatment P0, P1, P2 and P3 respectively w...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86493452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Imam Bachtiar, M. Ghafari, I. Rahman, B. Hilda, Mahrus
Coral symbiont diversity is one of the most important factors in coral resistance and coral reef resilience to bleaching. Many corals host only one symbiont clade, thus many of them vulnerable to climate change induced bleaching. Corals in Alas Strait have survived from several bleaching events. The present study aimed to look at genetic factors that potentially contributed to the survival of Echinoporalamellosa coral. Previously, E. lamellosawas reported to host only one clade of symbiont, either in Pacific or Indian Oceans. Here we provide new evidence on the ability of E. lamellosain hosting multiple clades of symbionts. The symbiont rDNA was extracted from small E. lamellosafragments and amplified by targeting ‘the non-coding gene region’ of ITS1-5.8S-ITS2. The clustering technique was used to determine the clade type of symbiont, by constructing the phylogenetic tree consist of the sequenced samples and downloaded sequences from GenBank NCBI as comparison. The constructed phylogenetic tree was based on Maximum Likelihood (ML) with bootstrap value 1000× to get the best result. The result shows the symbiont in E. lamellosatissue belonged to clades B and C. The combination of the two symbiont clades has never been recorded in previous studies, and it is very likely to have contributed to coral survival from bleaching events.Coral symbiont diversity is one of the most important factors in coral resistance and coral reef resilience to bleaching. Many corals host only one symbiont clade, thus many of them vulnerable to climate change induced bleaching. Corals in Alas Strait have survived from several bleaching events. The present study aimed to look at genetic factors that potentially contributed to the survival of Echinoporalamellosa coral. Previously, E. lamellosawas reported to host only one clade of symbiont, either in Pacific or Indian Oceans. Here we provide new evidence on the ability of E. lamellosain hosting multiple clades of symbionts. The symbiont rDNA was extracted from small E. lamellosafragments and amplified by targeting ‘the non-coding gene region’ of ITS1-5.8S-ITS2. The clustering technique was used to determine the clade type of symbiont, by constructing the phylogenetic tree consist of the sequenced samples and downloaded sequences from GenBank NCBI as comparison. The constructed phylogenetic tree was based ...
{"title":"Coral Echinoporalamellosa hosts multiple clades of symbionts in Western Alas Strait, Indonesia","authors":"Imam Bachtiar, M. Ghafari, I. Rahman, B. Hilda, Mahrus","doi":"10.1063/1.5141327","DOIUrl":"https://doi.org/10.1063/1.5141327","url":null,"abstract":"Coral symbiont diversity is one of the most important factors in coral resistance and coral reef resilience to bleaching. Many corals host only one symbiont clade, thus many of them vulnerable to climate change induced bleaching. Corals in Alas Strait have survived from several bleaching events. The present study aimed to look at genetic factors that potentially contributed to the survival of Echinoporalamellosa coral. Previously, E. lamellosawas reported to host only one clade of symbiont, either in Pacific or Indian Oceans. Here we provide new evidence on the ability of E. lamellosain hosting multiple clades of symbionts. The symbiont rDNA was extracted from small E. lamellosafragments and amplified by targeting ‘the non-coding gene region’ of ITS1-5.8S-ITS2. The clustering technique was used to determine the clade type of symbiont, by constructing the phylogenetic tree consist of the sequenced samples and downloaded sequences from GenBank NCBI as comparison. The constructed phylogenetic tree was based on Maximum Likelihood (ML) with bootstrap value 1000× to get the best result. The result shows the symbiont in E. lamellosatissue belonged to clades B and C. The combination of the two symbiont clades has never been recorded in previous studies, and it is very likely to have contributed to coral survival from bleaching events.Coral symbiont diversity is one of the most important factors in coral resistance and coral reef resilience to bleaching. Many corals host only one symbiont clade, thus many of them vulnerable to climate change induced bleaching. Corals in Alas Strait have survived from several bleaching events. The present study aimed to look at genetic factors that potentially contributed to the survival of Echinoporalamellosa coral. Previously, E. lamellosawas reported to host only one clade of symbiont, either in Pacific or Indian Oceans. Here we provide new evidence on the ability of E. lamellosain hosting multiple clades of symbionts. The symbiont rDNA was extracted from small E. lamellosafragments and amplified by targeting ‘the non-coding gene region’ of ITS1-5.8S-ITS2. The clustering technique was used to determine the clade type of symbiont, by constructing the phylogenetic tree consist of the sequenced samples and downloaded sequences from GenBank NCBI as comparison. The constructed phylogenetic tree was based ...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"45 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88465759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marliadi Susanto, M. U. Romdhini, S. Kamali, Laya Zurfani
Abalon (Haliotisasinina) is a marine animal that is classified in shellfish and belongs to the class of gastrophoda, family haliotidae. It is one of the mainstays of NTB and national ethnofauna as marine commodities for export. The existence of these snails has provided an important role for the economy of coastal communities not only eaten or sold in the local market but also exported to several Asian countries, Europe and the United States. Thus, the growth of Abalone is very important to know quickly so that the process of abalone cultivation becomes more effective. Abalone growth is a logistical problems, Abalone growth can be observed through changes in weight, length and biomass. this study focused on the growth of abalone length as a continuation of previous research. The length of Abalone is also one of the parameters of successful cultivation and marketing. It should be noted that knowing the growth of Abalone is quiteslows so this also becomes an interesting phenomenon to study. Slow growth is certainly one of the economic problems because it is related to the time of marketing or harvesting. One way to know quickly the growth of abalone is to construct the mathematical modelso that based on the results of analyzing the model can be known the optimal length and time of harvesting. The mathematical model constructed in this case is the Von Berlatanffy model as a generalization of the Verhulst logistics model. Based on the results of analysis of the model obtained the growth of the length of the Abalone reaches a maximum of 12 cm at the age of 80 months. However, harvesting at that age is less effective because it will require more maintenance costs so that the optimal harvesting time is 26 months with an abalone length of 8 cm and the length is said to be the optimal length because it meets national or international market standards.Abalon (Haliotisasinina) is a marine animal that is classified in shellfish and belongs to the class of gastrophoda, family haliotidae. It is one of the mainstays of NTB and national ethnofauna as marine commodities for export. The existence of these snails has provided an important role for the economy of coastal communities not only eaten or sold in the local market but also exported to several Asian countries, Europe and the United States. Thus, the growth of Abalone is very important to know quickly so that the process of abalone cultivation becomes more effective. Abalone growth is a logistical problems, Abalone growth can be observed through changes in weight, length and biomass. this study focused on the growth of abalone length as a continuation of previous research. The length of Abalone is also one of the parameters of successful cultivation and marketing. It should be noted that knowing the growth of Abalone is quiteslows so this also becomes an interesting phenomenon to study. Slow growth is c...
{"title":"Logistic model of abalon’s length growth in Sekotong, West Lombok","authors":"Marliadi Susanto, M. U. Romdhini, S. Kamali, Laya Zurfani","doi":"10.1063/1.5141285","DOIUrl":"https://doi.org/10.1063/1.5141285","url":null,"abstract":"Abalon (Haliotisasinina) is a marine animal that is classified in shellfish and belongs to the class of gastrophoda, family haliotidae. It is one of the mainstays of NTB and national ethnofauna as marine commodities for export. The existence of these snails has provided an important role for the economy of coastal communities not only eaten or sold in the local market but also exported to several Asian countries, Europe and the United States. Thus, the growth of Abalone is very important to know quickly so that the process of abalone cultivation becomes more effective. Abalone growth is a logistical problems, Abalone growth can be observed through changes in weight, length and biomass. this study focused on the growth of abalone length as a continuation of previous research. The length of Abalone is also one of the parameters of successful cultivation and marketing. It should be noted that knowing the growth of Abalone is quiteslows so this also becomes an interesting phenomenon to study. Slow growth is certainly one of the economic problems because it is related to the time of marketing or harvesting. One way to know quickly the growth of abalone is to construct the mathematical modelso that based on the results of analyzing the model can be known the optimal length and time of harvesting. The mathematical model constructed in this case is the Von Berlatanffy model as a generalization of the Verhulst logistics model. Based on the results of analysis of the model obtained the growth of the length of the Abalone reaches a maximum of 12 cm at the age of 80 months. However, harvesting at that age is less effective because it will require more maintenance costs so that the optimal harvesting time is 26 months with an abalone length of 8 cm and the length is said to be the optimal length because it meets national or international market standards.Abalon (Haliotisasinina) is a marine animal that is classified in shellfish and belongs to the class of gastrophoda, family haliotidae. It is one of the mainstays of NTB and national ethnofauna as marine commodities for export. The existence of these snails has provided an important role for the economy of coastal communities not only eaten or sold in the local market but also exported to several Asian countries, Europe and the United States. Thus, the growth of Abalone is very important to know quickly so that the process of abalone cultivation becomes more effective. Abalone growth is a logistical problems, Abalone growth can be observed through changes in weight, length and biomass. this study focused on the growth of abalone length as a continuation of previous research. The length of Abalone is also one of the parameters of successful cultivation and marketing. It should be noted that knowing the growth of Abalone is quiteslows so this also becomes an interesting phenomenon to study. Slow growth is c...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86350369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the exploration of eaglewood species in Buru Island, Maluku Islands, Indonesia on February 1-5, 2017, three noteworthy eaglewood species were found. One species was Gyrinopsmoluccana(Miq.) Baill in Namrinat village and Tikbary village, Namrole district, South Buru. This species has been mentioned in 1959 by Hou and Keliopas in 2013, however they were found in Kayeli area, North Buru. Three other species of eaglewood were new records to Buru. Two newly recorded species, first Gyrinopsdecipiens Ding Hou that was found at Waeduna Mount, Bara village, Aer Buaya district, North Buru and second GyrinopssalicifoliaRidl. from Tikbary village, Namrole district, South Buru. One genus was a new record to Buru island, namely Aquilaria. Its species was Aquilarifilaria(Oken) Merr.that was found together with G. salicifolia in Tikbary village, Namrole district, South Buru.In the exploration of eaglewood species in Buru Island, Maluku Islands, Indonesia on February 1-5, 2017, three noteworthy eaglewood species were found. One species was Gyrinopsmoluccana(Miq.) Baill in Namrinat village and Tikbary village, Namrole district, South Buru. This species has been mentioned in 1959 by Hou and Keliopas in 2013, however they were found in Kayeli area, North Buru. Three other species of eaglewood were new records to Buru. Two newly recorded species, first Gyrinopsdecipiens Ding Hou that was found at Waeduna Mount, Bara village, Aer Buaya district, North Buru and second GyrinopssalicifoliaRidl. from Tikbary village, Namrole district, South Buru. One genus was a new record to Buru island, namely Aquilaria. Its species was Aquilarifilaria(Oken) Merr.that was found together with G. salicifolia in Tikbary village, Namrole district, South Buru.
{"title":"New and noteworthy records of eaglewood from Buru, Moluccas, Indonesia","authors":"T. Mulyaningsih, I. Yamada","doi":"10.1063/1.5141283","DOIUrl":"https://doi.org/10.1063/1.5141283","url":null,"abstract":"In the exploration of eaglewood species in Buru Island, Maluku Islands, Indonesia on February 1-5, 2017, three noteworthy eaglewood species were found. One species was Gyrinopsmoluccana(Miq.) Baill in Namrinat village and Tikbary village, Namrole district, South Buru. This species has been mentioned in 1959 by Hou and Keliopas in 2013, however they were found in Kayeli area, North Buru. Three other species of eaglewood were new records to Buru. Two newly recorded species, first Gyrinopsdecipiens Ding Hou that was found at Waeduna Mount, Bara village, Aer Buaya district, North Buru and second GyrinopssalicifoliaRidl. from Tikbary village, Namrole district, South Buru. One genus was a new record to Buru island, namely Aquilaria. Its species was Aquilarifilaria(Oken) Merr.that was found together with G. salicifolia in Tikbary village, Namrole district, South Buru.In the exploration of eaglewood species in Buru Island, Maluku Islands, Indonesia on February 1-5, 2017, three noteworthy eaglewood species were found. One species was Gyrinopsmoluccana(Miq.) Baill in Namrinat village and Tikbary village, Namrole district, South Buru. This species has been mentioned in 1959 by Hou and Keliopas in 2013, however they were found in Kayeli area, North Buru. Three other species of eaglewood were new records to Buru. Two newly recorded species, first Gyrinopsdecipiens Ding Hou that was found at Waeduna Mount, Bara village, Aer Buaya district, North Buru and second GyrinopssalicifoliaRidl. from Tikbary village, Namrole district, South Buru. One genus was a new record to Buru island, namely Aquilaria. Its species was Aquilarifilaria(Oken) Merr.that was found together with G. salicifolia in Tikbary village, Namrole district, South Buru.","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"138 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86508600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}